THRESHOLD FEAR CONDITIONING
Contents
1. ASSOCIATES INC THRESHOLD Using the MED PC IV Load Wizard MED PC IV is designed to help the researcher run an experiment by guiding selection choices through its Experiment Loading Wizard This section will describe how to initiate the Fear Test 1 mpc application however the following steps that will also apply to all other mpc procedures Open MED PC IV and the MED PC Experiment Loading Wizard s Welcome screen shown in Figure 3 2 will appear Figure 3 2 The MED PC IV Loading Wizard Welcome Screen To avoid this load wizard deselect the checkbox labeled Run this experiment automatically when starting MED PC Close this screen by clicking the Close button Closing this screen immediately reveals the MED PC Run Time Screen shown in Figure 3 9 If the choice to continue with the Loading Wizard is made then click the Next button MED ASSOCIATES INC THRESHOLD The Box Selection screen will appear next as shown in Figure 3 3 From this screen the researcher chooses which boxes will be used in the experiment Select the boxes that will run the experiment by clicking in the radio button next to the box number The figure shows that the Hardware Configuration included only 1 box which was selected Click Next to continue Figure 3 3 The Box Selection Screen The Select a Procedure screen appears next as seen in Figure 3 4 This is where the application to be run is selected The screen displays a list of all the2. currently compiled procedures Select the desired procedure and then click Next Figure 3 4 The Select a Procedure Screen FEAR TEST 1 FEAR TEST 2 FEAR TRAINING 1 FEAR TRAINING 2 NES ECS ECS MED ASSOCIATES INC THRESHOLD The Enter Experiment Data Screen should display next as shown in Figure 3 5 The purpose of this screen is to allow annotations to be added to the data file that is produced by MED PC IV These annotations will help identify the Subject Experiment and Experiment Group upon which data was collected Comments can be added here as well and the data file can be given a customized file name to help identify it from other data files Enter the information desired and click Next Figure 3 5 Enter Experiment Data Screen Subject Subject_1 Experiment Theshed Group Group 1 Comments Threshold Experiment Optional Custom Filename Thresheld Subject Group1 The next screen to appear is the Review Choices screen as seen in Figure 3 6 This is a method of confirming that the information received from the Box Procedure Selected is correct If it is not correct select Previous and edit the data If it is correct select Next Figure 3 6 Review Choices Screen Review the data below Box 1 Procedure FEAR TEST 1 Subject Subject_1 Experiment Threshold_1 Group Group_1 Comment Threshold_Experiment Filename Threshold_Subjectl_Group1 Click Next to finish loading the box or Previ
3. particular type of interface card that is supplied in the interface cabinet may vary please refer to the User s Manual provided for instructions on how to configure the type of card that is in the cabinet A valid Hardware Configuration must exist in order for MED PC IV to interface correctly with the MED Associates Inc hardware This means that one should take the time to create a valid Hardware Configuration before attempting to run the procedures included in this package Should there be any problems the staff at MED Associates Inc is available to answer any questions that may arise Please e mail us at support med associates com with a detailed description of the problem or desired goals so that concise and detailed information may be provided The Threshold Fear Conditioning procedures are designed to be as easy to use as possible MED Associates Inc understands that researchers do not have the time to devote to programming and hardware design and for that reason we have undertaken that burden for you We sincerely hope that you are satisfied with the products and services we provide and look forward to meeting your future experimental needs as your research program evolves Overview of the Procedures This package contains two Fear Training procedures and two Fear Test procedures for use with the Threshold Fear Conditioning systems and Threshold Activity software The Fear Training 1 mpc and Fear Test 1 mpc procedures are writ
4. recorder is running Open Fear Test 1 mpc by clicking Files Open Session Change the variables using any of the methods described above When all settings have been made turn the recorder off again by using the main menu or tool bar Save the macro with a distinctive name The example in Figure 3 13 was named Fear Test 1 5min tone mac since the Tone on Time was changed to five minutes Figure 3 13 Fear_Test_1_5min_tone mac P Fear_Test_1_5min_tone MAC Notepad File Edit Format View Help LOAD Box 1 SUBI O EXPT O GROUP O PROGRAM FEAR TEST 1 SET Tone On Time min VALUE 5 MAINBOX 1 BOXES 1 Once this macro is built use the macro editor to make simple changes such as replacing reward or correct lever values Review the Help file on screen or the MED PC IV User s Manual for more information on macros and the features offered A START command or message box followed by a START command could be added to the macro it was left off here so changes could be verified before starting the procedure MED ASSOCIATES INC THRESHOLD Modifying the MedState Notation Code Permanent changes to the Threshold procedures can be made to the MedState Notation code To make the same change to the Tone on Time as shown above do the following Open Trans IV and select File Open to place Fear Test 1 mpc into the text editor Scroll down to approximately line 37 note the line counter in the lower right hand corner of the editor to reve
5. to Excel might ever be used Data file formats are explained in detail in the MED PC IV User s Manual Sample Data File Select Annotated on the file options page during hardware installation to produce a raw data file similar to the following Data files are located in C MED PC IV Data unless an alternate path was defined during hardware installation They may be opened with note pad word pad or any word processor however make sure they are always saved unformatted in the occasion a data extraction utility such as MPC2XL is used Start Date 01 07 09 End Date 01 07 09 Subject 0 Experiment 0 Group 0 Box 1 Start Time 16 01 45 End Time 16 04 02 MSN Fear Test 1 A 2800 000 Frequency of the Tone Hz B 85 000 Amplitude of the Tone dB C 10 000 Rise Fall Time of the Tone ms D 0 000 Not Used E 3 000 Baseline Time min F 10 000 Tone on Time min G 0 000 Not Used H 0 000 Not Used I 0 000 Not Used J 0 000 Not Used K 0 000 Not Used L 0 000 Not Used M 0 000 Not Used N 0 000 Not Used O 0 000 Not Used P 0 000 Not Used Q 0 000 Not Used R 0 000 Not Used S 0 000 Elapsed Time in Session T 0 000 Not Used U 0 000 Not Used V 0 000 Not Used W 0 000 Not Used X 0 000 Not Used Y 0 000 Not Used Z 0 000 Not Used
6. THRESHOLD FEAR CONDITIONING MED STATE NOTATION PROCEDURE SOF 700RA 14 Manual DOC 142 Rev 1 1 Copyright 2009 All Rights Reserved MED Associates Inc P O Box 319 St Albans Vermont 05478 www med associates com MED ASSOCIATES INC THRESHOLD TABLE OF CONTENTS Chapter 1 422g NG EEEEEEEEEEENNENEEENUESEREFEREEEEREREREEREREREREEREERFERFEIREERRR 1 Introduction une ae nee 1 Overview of the Procedures aaaaaaaaaananananan nana NENE NANANA ANAN ENGR nenne nn nanana nananana 2 Chapter 2 SPPERPRPEFFPEPPEPEEEEPEPEPEEEEPEEEPEEPEPEEEPEREEREREEFEEFFEFFEFEEEPEFFEFEEFFEFFEPFEEPEPERRREUER 3 Getting Started nace aaea DENG DARA een 3 Software Installation Gn ne NG ENG deter eae dann 3 Backing Up the Software 3 Chapter 3 mGA 4 Beginning Running an Experiment 4 Translating the MED PC IV mpc File 4 Using the MED PC IV Load Wizard een nn 5 Viewing Changing Variable Values sssssssserenerensennnnnnenenen ernennen nenn 10 M CLOS ua Re la PNG tes GD DAG NG hai 11 Modifying the MedState Notation Code 12 Chapter 4 PAP ET SERETERFEREER 13 Understanding the Data Files 13 Sample Data FE sen ra canine teed 13 MED ASSOCIATES INC THRESHOLD CHAPTER 1 Introduction The purpose of this manual is to give an explanation of the MED State Notation Procedures that comprise the SOF 700RA 14 Threshold Fear Conditioning Procedures The files in this package can be found o
7. al the code shown in Figure 3 14 Figure 3 14 Fear Test 1 mpc Line 37 ER TRANS IV C MED PC IV MPC Fear Test 1 mpc EIER a x Kag File Edit Search Options Translation Window Help gt 5 5 1 SL Set Variables with Default Values for the Tone and Timing 0 001 SET 2600 B 85 C 10 SET E 3 B gt s2 Change F 3 to F 5 and save the changes with the same or a new file name such as Fear Test 1 5min tone mpc Remember if creating a new mpc file name and are using a macro to load boxes the file name in the macro also must be changed Translate and compile the new or changed file as described previously and run MED PC IV Use the Change Variables screen to view confirm the new values CHAPTER 4 Understanding the Data Files Unless otherwise specified data will be saved to C MED PC IV DATA Data can be saved manually by selecting FILE SAVE DATA MANUALLY or FILE SAVE DATA FLUSH The file name that is used to save the data in depends on the option that was chosen in the Hardware Configuration Utility and may also be dependent on the Subject Experiment and Group name provided in the MED PC IV load wizard Within each data file the headings are created for each Subject Experiment Group Box etc see below Data files may be opened with note pad word pad or any word processor or spreadsheet however be sure they are always saved unformatted in case a data extraction utility such as MED PC
8. ick Install Threshold Fear Conditioning The procedures are copied into the C MED PC IV MPC folder Backing Up the Software Med Associates strongly encourages creating backup copies of the programs in case of disk failure Having copies of the original programs may be useful in the future should modifications be made to the existing programs CHAPTER 3 Beginning amp Running an Experiment Translating the MED PC IV mpc File Programs written in MedState Notation must be translated using Trans IV before they can be executed in this application Be sure that a copy of the file being translated is present in the directory C MED PC IV MPC Open Trans IV icon and select Translation Translate and Compile Select the program s to use for the experiment and click Make Click OK to start the translator and it will automatically parse the MedState Notation and then open to a DOS screen to compile the Pascal code Depending on the speed of the computer each of these steps may not be seen If any problems are encountered during this process refer to the on screen help menu or the MED PC Version IV User s Manual or contact MED Associates Inc for assistance Figure 3 1 Trans IV Control Panel for Translating and Compiling MedState Notation Code ih Specify Files to Translate Fear Test 1 mpc Fear Test 2 mpc Fear Training 1 mpc Fear Training 2 mpc Build No Trans Exclude OK X Cancel Help MED
9. iew Macros Help lole jale 8 Elm e Box Subject Experiment Group Load Program 1 0 0 0 14 49 Fear Test 1 l2 EN fa 15 le 7 le ls boli fiatishafististi7 aaa Frequency Hz 2800 00 Amplitude dB 85 00 Rise Fall ms 10 00 Baseline min 3 00 Tone On min 3 00 SHOWS 6 10 SHOWS 11 15 SHOWS 16 20 SHOWS 21 25 SHOWS 26 30 SHOWS 31 35 1 7 2009 2 49 24 PM Eile A Wizard for Loading Boxes Gr Open Session Ctrl O 1 MED ASSOCIATES INC THRESHOLD Viewing Changing Variable Values Before a start command has been issued any variable may be changed on the MED PC IV run time screen Simply highlight the value to change and then enter the new value Once a session is in progress change variables by selecting Configure Change Variables or click the 4th tool bar item AX In the lower left hand corner of the Change Variables window find the Display Data from Box display and choose the chamber s to modify By clicking additional boxes in the Additional Boxes to Update section changes made to a single box are automatically loaded to all of the selected boxes Figure 3 11 Changing Variables Screen Displaying Variables from Box 1 2800 000 0 000 3 000 2 F k J Vars 3 000 0 000 0 000 K N o 0 000 0 000 0 000 Heres P S T 0 000 0 000 0 000 Issue U x Y Ci 0 000 0 000 0 000 ose z 0 000 Hep Displa
10. n the disk provided by MED Associates Inc These procedures are intended to be run in MED Associates MED PC IV software The latest version of MED PC IV gives researchers the ability to use pre programmed procedures such as these to make hardware control and data collection easy These pre programmed procedures can also be modified to meet the evolving demands of a research protocol Again it is the intent of this manual to explain exactly what these procedures implement and provide guidance into how to interpret what the program code achieves in order to let the user determine how to modify them to match their research protocol demands The manual provides some examples of editing and modifying the procedure s programming code The manual also defines the elements in the raw data file produced by these procedures In addition to this manual refer to the MED PC IV User s Manual for the installation of the MED Associates interface drivers the MED PC IV Software and the Delphi Compiler Also refer to the User s Manual for instructions on developing a Hardware Configuration Data file structure file saving format and other related options are also determined by the Hardware Configuration Running the Hardware Configuration software utility that accompanies MED PC IV sets the Hardware Configuration Its purpose is to assign the inputs and outputs on the interface cards in the interface cabinet for each task controlled by MED PC IV The
11. ous to correct errors MED ASSOCIATES INC THRESHOLD The Alter Session Parameters Screen shown in Figure 3 7 is the next screen to appear and is an important screen for the researcher The Alter Session Parameters screen allows the researcher to alter the parameters by which a procedure executes The Send Start Command Screen appears next The options available on the screen vary depending upon how many boxes are described in the Hardware Configuration Figure 3 7 Alter Session Parameters Screen Frequency of the Tone 2 Amplitude of the Tone dB Rise Fall Time of the Tone ms Baseline Time rin Tone On Time min In this example only 1 box is described in the Hardware Configuration so Figure 3 8 will appear next If more than 1 box is in the Hardware Configuration then Figure 3 9 will appear Figure 3 8 Send Start Command Screen for Single Box Configuration MED ASSOCIATES INC THRESHOLD Figure 3 9 Send Start Command Screen for Multiple Box Configuration In both cases Figure 3 8 and Figure 3 9 the screens are where the researcher decides to either load more boxes send a start signal to boxes that are already loaded or enter the MED PC IV run time environment without sending a start signal by selecting am finished with the wizard This option results in the screen shown in Figure 3 10 Figure 3 10 The MED PC IV Run Time Screen MED PC IV lolx Fie Configure V
12. ten for systems equipped with the Programmable Audio Generator ANL 926 and all of the procedures are written for systems equipped with the Computer Controlled Constant Current Aversive Stimulator ENV 413 Fear Training 1 mpc is a standard training protocol that waits 120 seconds after the start is issued to turn on a tone for 30 seconds During the last two seconds of the tone the foot aversive stimulation is turned on The tone and the foot aversive stimulation are turned off simultaneously The animal is left in the chamber for an additional 30 seconds Fear Training 2 mpc is an alternative training protocol that waits 30 seconds after the start is issued to turn on a 2 second foot aversive stimulation After waiting 60 seconds a second 2 second foot aversive stimulation is issued The animal is left in the chamber for an additional 30 seconds Fear Test 1 mpc is a conditioned stimulus test protocol that has a 3 minute baseline followed by a 3 minute tone Fear Test 2 mpc is a no conditioned stimulus test protocol that runs for 5 minutes CHAPTER 2 Getting Started Software Installation Please refer to the MED PC IV User s Manual for a complete guide to installing the MED PC IV software building a valid Hardware configuration with the Hardware Configuration utility and opening and compiling a MSN procedure in the Trans IV utility To install the Threshold Fear Conditioning Procedures insert the CD into the CD ROM drive and cl
13. y Data from Box Additional Boxes to Update AfromBox1 2800 000 3c Select All Deselect All To change a value simply highlight and replace the value in the lower right hand box or use the up down arrows to increment by 1 Click the Issue button for the change to take effect Click Named Variables to produce the display in Figure 3 12 Change variables here as needed Figure 3 12 Displaying Named Variables from Box 1 Displaying Named Variables from Box 1 Frequency of the Tone Hz 2800 000 Amplitude ofthe Tone dB Rise Fall Time of the Tone ms Baseline Time min Named Vars Vars Refresh Tone On Time min i Issue Close wuld Help Display Data from Box Additional Boxes to Update oy mi Select All Deselect All LOs Macros The simplest way to initially create a macro is to record keyboard functions while performing the steps manually Once the commands are in the macro it is easy to create a number of macros with the macro editor The following example illustrates the process of loading Box 1 and changing the Tone on Time to five minutes To begin open MED PC IV and going directly to the run time screen Close the load wizard if present Before loading or opening the procedure click Macro on the main menu and select Turn On Macro Recorder or click the 8th tool bar item with the cassette tape icon on top A note on the bottom of the display indicates that the
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