User`s Manual
Contents
1. View Caltr ction Cote Start Data Capture 21 48 Y 0s 16009 10 35 29 amp 44 76 8 9 00 al ej BP stort BD Onives Donova E Mariel ond trot W writiied Pont I C Soft Manager BY Meroscht Word 4020 842 MAMUWO msa Waveform Screen Users can graphically view real time data for each sensor by clicking the Display Waveform box Channel names and units will be displayed on the Y axis Time and date will be displayed on the X axis Clicking the Autoscale box will automatically scale graphs Users may also rescale graphs by unchecking the Autoscale box and manually entering desired values for either Y or X axis ranges Data can be saved during waveform mode by clicking Start Data Capture Data files will be saved as csv format File Settings COM Port Help C6 Multi Sensor Platform Connected l Data Log Chart Display Waveform IV Autoscale IV a n Settings Current Data Log Setup Download Data h y Wiman A d s AAAA Wy w Vv Wir wi i Wy VV Wa BA A Curent Data t Chan 1 Chlorophyll a 15 52 chan 2 Brighteners 14 28 Calibrate Chan 3 Fluorescein 35 76 Calibrake Chan 4 CDOM 12 8 Calibrate Calibrate Chan 5 Phycocyanin 65 64 chan 6 Of 23 6 Calbrate Depth meters 0 Calibrate Temp C 23 1 View Calbration Data Start Data Capture 998 2300 Rev K Page 13 C3 Submersible Fluorometer Calibration Screen Users can calibrate senso2. 6 Brown DR amp DsR Prae 7 Yellow RTS amp CTS PINT BO 8 Orange NA NA Power ground and V Batt are not common 998 2300 Rev K Page 2 C3 Submersible Fluorometer Computer Interface Cable P N 2200 150 This cable is included as a standard accessory with the C3 It is used to program and communicate with the C3 using C Soft and provide real time data view This cable is NOT recommended for serial data output The cable length is approximately 2 5 feet Integration Interface Adapter Cable P N 2300 115 This cable is included as a standard accessory included with the C3 This cable works with the Computer Interface Cable P N 2200 150 It allows users to upload firmware to the instrument using the USB or stream digital data continuously using the RS232 plug Either plug can be used to communicate and configure the instrument OBSOLETE Computer Integration Cable P N 2200 160 and 2200 165 This can be identified by the yellow band of heat shrink The integration cable is used to connect the C3 to a PC or data logger for continuous data capture This cable is recommended for serial data output as it prevents interruptions in the C3 data transmission to the PC Replaced by P N 2300 115 12 VDC Power Supply P N 159 0191 This is included as a standard accessory with the C3 The power supply connects to the power connector on the interface or integration cable which will supply pow
3. Appendix D Linear Range Quenching and Temperature Considerations The linear range is the concentration range in which the C3 sensor output is directly proportional to the concentration of the signal The linear range begins with the smallest detectable concentration and spans to an upper limit concentration that is dependent upon the properties of the material filters used and path length A non linear relationship is seen at very high concentrations where the signal does not increase at a constant rate in comparison to the change in concentration see figure below At even higher concentrations the signal will decrease even though the sample concentrations are continuing to increase This effect is Known as signal quenching Linearity can be checked by diluting a sample 1 1 or some other convenient ratio If the sample is still in the linear range the reading will decrease in direct proportion to the dilution If the reading does not decrease in direct proportion to the dilution or if the reading increases the sample is beyond the linear range Fluorometer Response Curve Graph showing Linear and Quenching Regions of the sample s response Sample __ Linear Region Temperature Considerations Fluorescence is temperature sensitive As the temperature of the sample increases the fluorescence decreases For greatest accuracy record the sample temperature and correct the sensor output for changes in temperature For
4. Flow Cap installation is complete OUT FLOW Siaa l PORT i NOTE For optimal T IN FLOW use Turner Designs PORT recommends positioning the C3 unit vertically when the Flow Cap is installed This will purge any air from the system that might cause skewed readings Rev J Page 39 C3 Submersible Fluorometer Calibration Cup Use With the C3 Submersible Fluorometer s Flow Cap Housing installed 1 Remove the End Cap 2 Use a port plug to plug the port located on Port Plug the Flow Cap Housing 3 Position the unit vertically with optical head facing upward see diagram to the right In this position the Flow Cap Housing can be used to hold standard solutions for calibrating the C3 sensors Specifications Max Pressure 100 psi Length 10 1 cm 3 98 in Diameter 10 cm 3 94 in Weight 455 g 1 003 Ibs Material Delrin Max Volume 400 ml 998 2300 Rev J Page 40 C3 Submersible Fluorometer Appendix J Using the Solid Secondary Standard Introduction Installation The following information describes how to use the Solid Secondary Standard Cap and Solid _ Align Solid Secondary Standard Cap Secondary Standard inserts for the C3 numbers with the C3 s numbers located Submersible Fluorometer on the optical head and snap on the Solid Secondary Standard Cap P N 2300 905 Solid Secondary Standard Cap P N 2300 901 Solid Secondary Standard insert for In Vivo Chlorophy
5. Step 4 Optional Temperature Compensation Temperature compensation is optional it enables the C3 to compensate for changes in fluorescence due to varying temperatures as detected by the C3 s temperature sensor Temperature compensation is available for in vivo Chlorophyll and Rhodamine sensors only Temperature is displayed in degree Celsius Click on the Temperature Compensation box Select either in vivo Chlorophyll or Rhodamine from the dropdown menu Rev K Page 15 3 3 2 998 2300 C3 Submersible Fluorometer Data will now be corrected for temperature fluctuations The temperature compensation coefficients for each fluorophore are listed in the table below Fluorophore Coefficient Rhodamine 0 026 C Exponential in vivo Chlorophyll 0 014 C Linear Step 5 Save Calibration After calibration is complete select Save Calibration Data are now displayed as concentration estimates Raw Fluorescence Calibration Blank Subtracted Following steps 1 5 below will ensure values are reported as Blank subtracted Relative Fluorescence Units RFUB See Appendix B for recommended practices Step 1 Blanking e Place the C3 Submersible Fluorometer in a blank solution Note A blank solution is a solution without the fluorophore of interest i e deionized water artificial water or filtered water Note Ensure the optical head is free of air bubbles Wait until Current RFU
6. Current Time 10 32 27 Set Unit To PC Date amp Time 6 16 2008 E Manually Set Unit Date amp Time Cancel Set Unit Clock 3 2 2 Current Data and Calibration Screen 998 2300 The Current Data Screen allows users to view real time data acquisition in both tabular and waveform formats as well as conduct calibrations for each channel and view calibration information Current Data Channel names are displayed but cannot be changed in the Current Data screen Real time values are displayed next to each channel and in the adjacent Data Table A calibration button is available for each channel and the depth sensor Real time data can be saved by selecting the Start Data Capture button Users will be prompted to enter a file name and file location Data files will be saved as csv format Tabular Screen Real time data including date time units and temperature are continuously scrolling on the tabular screen for enabled channels Channel names will appear in the associated column headings If units are not selected during calibration a relative fluorescence units RFU label will appear Rev K Page 12 C3 Submersible Fluorometer Fle Settings COMPot corvetes Data Table Display Waveform 7 to Tene Ahad Pintaxyoa Depth Temo R f meters Scllings Curent Data LogSetup Download Data l Curent Dat Chan i Rhodamine 04s Calibe abe aire Depth meters o Coltrate
7. Edit View Call Transfer Help Send File Receive File mma Send Text File Pause Resume Capture to Printer T 998 2300 Rev J Page 48 C3 Submersible Fluorometer Appendix M Integrator Firmware Turner Designs offers Integrator Firmware This firmware is designed for use by customers that are integrating their C3 with an external datalogger CTD or similar system The Integrator Firmware is different from the standard C3 firmware and has some features that users need to consider before installing 1 Internal datalogging is NOT possible with this version of firmware The Integrator Firmware is intended for data output only 2 The data output rate defined by this Integrator Firmware is 1 second and cannot be changed to reflect longer or shorter data output rates 3 Once power is applied there is a 15 second delay before data output starts at a 1 second interval 4 Ifthe wiper is enabled it will rotate the set number of rotations upon powering the instrument and every 5 minutes as long as power is continuously supplied 5 Sleep mode is disabled therefore as long as power is continuously supplied the instrument will continue to stream data at a 1 second interval Download Firmware on the Turner Designs website http www turnerdesigns com customer care software a firmware 998 2300 Rev J Page 49 C3 Submersible Fluorometer Appendix N Campbell Scientific Datalogger Integration Turner Designs and Campbell S
8. Soft Manager Connected J File Settings COM Pork window Help 3 2 Software Operation 3 2 1 Settings Screen The Settings Screen allows users to set the time and date identify each optical sensor update serial numbers and enable disable sensors Users can manually set the date and time or choose to synchronize the C3 Submersible Fluorometer to the PC date and time Each channel 1 3 in the Settings Screen has a location associated with it on the C3 Submersible Fluorometer that is numerically labeled 1 3 respectively Channels 4 6 are designated for the C6 Multi Sensor Platform and are disabled for the C3 Click on the box adjacent to the channel to enable channel operation If there is no sensor installed users will be unable to select that channel Channels can be renamed using the dropdown selections or by manually entering a name The serial number boxes are designed for the C6 Multi Sensor Platform There is no serial number associated with the sensors in the C3 Settings will automatically be saved after exiting the Settings screen 998 2300 Rev K Page 11 C3 Submersible Fluorometer File Settings COMPort Window Help Settings Current Data Channel Settings Depth V Temp Enable Connected Log Setup Download Data C Soft Manager xj Name Serial Chani M Rhodamine Unit Current Time Chan 2 Chlorophyll a ae Retrieve Unit Time Chan3 M phycocyanin Chat
9. above the bottom of the container Ensure that the sensor is in the center of the container and has gt 2 inches clearance between the sensor and the inside surface of the container 5 If using a glass beaker check if the optical surface of the sensor is free of air bubbles Remove air bubbles if any are observed If using a dark plastic container run the wiper or your finger over the optical sensors to ensure the sensor heads are free of air bubbles Calibrated Sensor gt 2 inches all round Glass Beaker gt 998 2300 Rev K Page 26 C3 Submersible Fluorometer Appendix C Wiring and Cable Guide The C3 Submersible Fluorometer outputs digital data in three formats 1 Digital data that can only be read by C3 Submersible Fluorometer s software 2 Digital data in ASCII format that can be read by larger multi parameter systems such as CTD s and 3 Digital data that can be read and converted to analog data by the C series Analog Adapter An 8 pin Impulse cable provides two end connections 1 a 9 pin RS232 serial port for connection to a PC or laptop computer and 2 a 12V port for supplying power to the unit C3 Submersible Fluorometer bulkhead and serial port connectors OOO OOO Sub D Connector Wire Guide C3 Function Corresponding Sub D Connector Pin EREC V BATT Power Connector Cable Center Pin V BATT Power Connector Cable Housing 3 PIN 5 PIN 2 5 Blue TX PIN 3
10. can be used to establish a new correlation voltage without using a calibration solution each time 1 Using your C3 Submersible Fluorometer measure a dye solution with known concentration and record the response values for that measurement 2 Dry off the optical end of the C3 attach the Solid Secondary Standard Cap and insert to the fluorometer and adjust the Solid Secondary Standard to produce the same response as in step 1 3 The Solid Secondary Standard s signal is now equivalent to the concentration value of the dye solution used in step 1and can be used in place of a liquid primary standard for future calibration of that specific C3 Note Comprehensive information on dye trace measurements can be found at the following Turner Designs website http www turnerdesigns com flu orescent dye tracing Rev J Page 42 C3 Submersible Fluorometer Installation C3 with Wiper Specifications Solid Secondary Standard insert Remove the tightening nut and wiper arm from P N 2300 901 and 2300 902 the C3 Weight 45 g 0 099 Ibs Length 5 7 cm 2 25 inches Solid Secondary Standard Cap P N 2300 905 Now follow steps 1 4 for the installation of the Solid Standard and instructions on Solid Standard adjustment Care and Storage Solid Secondary Standards should be stored at room temperature 20 degree C in their case when not in use and kept free of dust and moisture Special care must be taken with the UV S
11. further information on how temperature light water quality and the physiological state of the algal cells can all affect the measurement of chlorophyll please refer to the application section of Turner Designs website 998 2300 Rev K Page 31 C3 Submersible Fluorometer Appendix E CFINS Installation Guide Requirements Turner Designs C3 Submersible Fluorometer with extender cable boosters required for cable lengths gt 10M ArcGIS Version 10 PC with two com RS232 serial ports or PCMCIA slot GPS with com port RS232 set to output NMEA and GPS serial cable ArcMap C3 Extension Steps Prepare C3 Fluorometer e Run C Soft and set up C3 for log sampling interval of 1 second See Log Setup Section 3 4 of C3 User s Manual e Initiate datalog session as per instructions in Section 3 4 of manual C Soft will automatically shut down e Power down and power up C3 it will take 90 seconds for data to start streaming Prepare ArcMap e If not already installed install C3 extension in ArcMap double click TD_C3 esriAddIn e Select both GPS Toolbar and Turner Designs C3 toolbar using Customize Toolbars 998 2300 Customize Windows Help Toolbars Extensions Geopracessing SD Analyst Advanced Editing Add In Manager Animation VBA Macros ArcSocan Customize Mode COG Data Driven Pages Style Manager Data Frame Tools rcMap Options Distribut
12. readings have stabilized then select the Set Blank button to set the blank value Wait for the blank value to be measured and set for all 3 gains Step 2 Selecting Units Select RFUB from the units dropdown menu Note Do not enter a value in the standard value box Step 3 Skip and go to Step 4 Step 4 Optional Temperature Compensation Follow same steps as the Direct Concentration Calibration procedure in Section 3 3 1 Step 5 Save Calibration After calibration is complete select Save Calibration Data are now displayed as blank corrected relative fluorescence units Rev K Page 16 C3 Submersible Fluorometer 3 3 3 Raw Fluorescence Calibration 3 3 4 998 2300 In the Current Data screen select the calibrate button next to a specific channel At the bottom of the Calibration Screen select Click to use uncalibrated Raw Fluorescence Mode Data for that channel will be uncalibrated and reported as Relative Fluorescence Units RFU Depth Calibration Optional Accessory Note Make sure your instrument is calibrated for depth and there is a value set even if you do not have a depth sensor Depth is displayed in meters only There are two calibration options for depth 1 The first uses only the atmospheric pressure as an offset This is suitable for any shallow application including most fresh water applications 2 The second also compensates for gravity variations
13. 00 901 2300 220 Fluorescein Dye 0 01 ppb 0 500 ppb 400 150 nm_ 545 28 nm 2300 901 2300 253 Oil Crude 0 2 ppb 0 2700 ppb 365nm 325 120nm 410 600 nm 2300 902 2300 255 Oil Fine 2 ppb 0 10 000 ppb 285nm lt 290nm 350 55nm 2300 902 2 ppm gt 100 ppm agoz Se Elie Wenner er 0 6 ppb 0 15 000 ppb 365mm 365mm 325120 325120 445 15nm 2300 902 Wastewater Treatment 2300 231 Phycocyanin Freshwater 2 Spb 0 40 000 ppb 590nm lt 595nm gt 630nm 2300 901 oe 1 5 Naphthalene Disulfonic Disodium Salt Quinine Sulfate PTSA 1 3 6 8 Pyrenetetrasulfonic Acid Tetrasodium Salt BTEX Benzene Toluene Ethylbenzene Xylenes Pe Phycocyanin pigment from Prozyme diluted in Deionized water http prozyme com PE Phycoerythrin pigment from Prozyme diluted in Deionized water http prozyme com 998 2300 Rev K Page 51
14. 4 1 2 Care for the bulkhead connector Install the 8 pin female end plug to the bulkhead between uses A light coat of Silicone spray should be used on the rubber of the male pins of the bulkhead to aid in sealing The manufacturer recommends 3M Silicone Lubricant Spray or Loctite 8021 spray Note You should avoid using silicone grease Do NOT use WD 40 it will destroy the connectors 4 2 Warranty Terms Turner Designs warrants the C3 and accessories to be free from defects in materials and workmanship under normal use and service for a period of 12 months from the date of shipment from Turner Designs with the following restrictions e Turner Designs is not responsible for replacing parts damaged by accident or neglect Your instrument must be installed according to instructions in the User s Manual Damage from corrosion is not covered Damage caused by customer modification of the instrument is not covered e This warranty covers only Turner Designs products and is not extended to equipment used with our products We are not responsible for accidental or consequential damages except in those states where this limitation is not allowed This warranty gives you specific legal rights and you may have other rights which vary from state to state e Damage incurred in shipping is not covered 998 2300 Rev K Page 22 C3 Submersible Fluorometer 4 3 Warranty Service To obtain service during the warranty period the owner shall take th
15. 8 2300 Rev K Page 14 C3 Submersible Fluorometer 3 3 Calibration Procedure 3 3 1 998 2300 Direct Concentration Calibration Following steps 1 5 below will ensure values are reported as blank subtracted Concentrations in units of choice See Appendix B for recommended practices Step 1 Blanking Place the C3 Submersible Fluorometer in a blank solution Note A blank solution is a solution without the fluorophore of interest i e deionized water artificial water or filtered water Note Ensure the optical head is free of air bubbles Wait until Current RFU readings have stabilized then select the Set Blank button to set the blank value Wait for the blank value to be measured and set for all 3 gains Step 2 Standard Value and Units Place the C3 Submersible Fluorometer in a standard solution or use a solid secondary standard See Appendix J for more information on solid secondary standards Note Turbidity sensors do not have solid secondary standards Enter the known concentration value of the standard solution or solid standard in the Standard Value box If the concentration is not known enter an arbitrary value Enter manually or select desired units from the dropdown menu Note Do not choose or enter RFUB if Direct Concentration Mode is preferred Step 3 Set Standard e When readings in Current RFU box have stabilized select the Set Standard button
16. N 2200 900 Note Required for cable lengths greater than 10 meters 24 Pigtail Interface Cable with Locking Sleeve P N 2200 170 Solid Secondary Standard Cap P N 2300 905 Solid Secondary Standard SSS for in vivo Chlorophyll Phycocyanin Phycoerythrin Rhodamine and Fluorescein P N 2300 901 see Appendix J for more information Solid Secondary Standard SSS for UV Sensors CDOM Optical Brighteners Refined Fuels and Crude Oil P N 2300 902 see Appendix J for more information Note Solid Secondary Standard not available for turbidity Analog Output Adapter P N 2300 480 see Appendix K for more information 998 2300 Rev K Page 6 C3 Submersible Fluorometer Flow Cap Shade Cap Air Purge Slots C ray Towed Deployment Body Battery Bracket Submersible Battery Mechanical Wiper Booster Kit Booster 1 Locking Solid Secondary Standard sleeve Booster 7 Solid Secondary Standard Cap 998 2300 Rev K Page 7 C3 Submersible Fluorometer 2 3 Identification Sensors are marked with numbers 1 2 or 3 located adjacent to the optics on the face of the C3 Submersible Fluorometer These numbers correspond to channels 1 2 and 3 in C Soft C3 software Note Channel names can be modified using C Soft Light emitting diode color table Blue Infra Red No Color Phycoerythrin Green Fluorescein f Pue See important note on page 5 Green CDOM Ultra Violet No Color R
17. TURNER D DESIGNS Reliable Instruments for an Unreliable World Submersible Fluorometer User s Manual April 30 2012 P N 998 2300 Revision K TURNER DESIGNS 845 W Maude Ave Sunnyvale CA 94085 Phone 408 749 0994 FAX 408 749 0998 C3 Submersible Fluorometer Table of Contents 1 Introduction 1 1 Introduction 1 2 Important Note Regarding the Use of the Fluorescein Sensor 2 Inspection and Setup 2 1 Instrument Checklist 2 2 Optional Accessories 2 3 Identification 3 C Soft Windows User Interface 3 1 Software Installation and PC Requirements 3 1 1 Minimum PC Requirements 3 1 2 Software Installation 3 1 3 Integration Interface Adapter Cable Installation 3 1 4 PC Software Interface 3 2 Software Operation 3 2 1 Settings Screen 3 2 2 Current Data and Calibration Screens 3 3 Calibration Procedures 3 3 1 Direct Concentration Calibration 3 3 2 Raw Fluorescence Calibration Blank Subtracted 3 3 3 Raw Fluorescence Calibration 3 3 4 Depth Calibration Optional Accessory 3 4 Log Setup Screen 3 5 Enabling Data Output Ready State 3 6 Downloading and File Management 4 Maintenance and Warranty 4 1 Maintenance 4 1 1 Rinsing 4 1 2 Care for the Bulkhead Connector 4 2 Warranty Terms 4 3 Warranty Service 4 4 Out of Warranty Service Appendices Specifications Recommended Practices Wiring and Cable Guide Linear Range Quenching and Temperature Considerations C FINS Installation Guide Installing the Ba
18. ake up to 10 minutes depending on file size When download has completed choose the files to be saved by clicking the Save box Users will be prompted to choose a folder location where data will be saved Files will be saved in csv format Select Clear Datalog to erase existing data from memory Users are able to graphically view downloaded csv data files using C Soft software if desired Corre 2 B C Salt Mauger xj Scie Cuneo la eji Diria Dila Select files to save Tota Fis Lid Pana Licey Pd an piunrge a i gure peenagen i i ny a baw HH HH w D MN Eui Thar Caw Data j 998 2300 Rev K Page 20 C3 Submersible Fluorometer Note If you have problems downloading data in C Soft you may have limited space in the cache memory on your computer If you have not done a Disk Cleanup for awhile you may want to try and see if that clears up the problem and allows you to download files Disk Cleanup can be found in the following location on most Windows versions Start Menu gt All Programs gt Accessories gt System Tools gt Disk Cleanup Once the initial scan of the computer is complete customers should select all the check boxes and select OK to perform the cleanup 998 2300 Rev K Page 21 C3 Submersible Fluorometer 4 Maintenance and Warranty 4 1 Maintenance 4 1 1 Rinsing The C3 Submersible Fluorometer should be rinsed or soaked in freshwater following each deployment
19. ancsl Cancel Data Point Note that flag only commits point once the OK button is pressed on user input Long delays in writing notes may influence location of flagged point 998 2300 Rev K Page 34 C3 Submersible Fluorometer e Logged data has the following fields C3 Temperature in degrees Celsius Chan1_ Unit PPB Channel 1 Units from C3 Setup Form Not C Soft Chan2_Type Turbidity Channel 2 Name from C3 Setup Form Not C Soft COG _True Course over ground in degrees true Flag Flag value 0 no flag 1 Flagged Channel names and units are logged as per the C3 setup form and not C soft These can be changed during a logging session but it is not recommended 998 2300 Rev K Page 35 C3 Submersible Fluorometer Appendix F Installing the Battery Bracket and Battery Introduction The Battery Bracket P N 2300 603 for the 4 Secure the connection by feeding the C3 Submersible Fluorometer was designed to Green Line through the holes on the provide support allowing a direct connection Battery Brackets base between the battery and the C3 during self contained deployment thus eliminating the Insert green line need for a cable connection The Battery provided to Bracket also provides balance and stability for secure the the C3 Submersible Fluorometer and battery connection Installation 1 Use the extra plastic green support line provided with the Battery Bracket to remove the installed plastic
20. and gravity offset Enter the latitude Step 3 Select Save Calibration button Depth values will now be scaled to the offset selected 3 4 Log Setup Screen The Log Setup screen allows the user to configure a logging session set the mechanical wiper to its home position and enable logging The C3 Submersible Fluorometer can log just over 64 000 lines of data Enter a log start date MM DD YYYY and time HH MM SS Enter the sampling interval HH MM SS The minimum sampling interval is 1 second If the mechanical wiper is installed click the Wiper box to enable Select the number of revolutions from the dropdown box 1 10 revolutions All sensors will be wiped at specified revolutions before each measurement Note If users select revolutions that exceed sampling interval a warning message will be displayed 998 2300 Rev K Page 18 C3 Submersible Fluorometer Note Do not click the Wiper Box to enable wiper if the wiper is not installed x The sampling interval is too short for the number of wiper revolutions You must choose a longer sampling time less revolutions or turn the wiper off Sampling Maximum Interval of Revs 1 10 0 11 20 21 30 31 40 41 50 51 60 gt 60 a UMNEe 0 Cancel After log set up is complete select Enable Datalog see section 3 3 Enabling Data Output Ready State A promp
21. ble Fluorometer Factory installed temperature sensor Interface cable for communicating with your PC and supplying power to the instrument Integration Interface Adapter Cable see Appendix M and N for more information about integration CD including C Soft software user s manual cable guide and quick start guide emperature Sensor 12V Power Supply Integration Interface Adapter Cable 998 2300 Rev K Page 5 C3 Submersible Fluorometer 2 2 Optional Accessories Pressure sensor P N 2300 360 Factory installed Note Can only be installed during the manufacturing of the unit C3 Mechanical Wiper P N 2300 450 Factory installed Note Can only be installed during the manufacturing of the unit see Appendix H for more information Replacement Wiper Brushes P N 2300 460 Replacement Wiper Spacers P N 2300 462 Rechargeable Submersible Battery Pack including charger P N 2200 601 see Appendix G for more information Battery Bracket P N 2300 603 Note Required for use with Battery Pack and Analog Output Adaptor see Appendix F for more information Flow Cap P N 2300 700 see Appendix I for more information Shade Cap P N 2300 500 or C ray Shade Cap P N 2300 502 Shade Cap Weight P N 2300 510 C ray Towed Deployment Body P N 2300 750 Extender Cables 10 meters P N 105 2595 25 meters P N 105 2596 50 meters P N 105 2597 Custom lengths available upon request Booster Kit P
22. cientific worked together to help our customers easily integrate the C3 Submersible Fluorometer with any of the CR800 or CR1000 series dataloggers Simply follow the steps below to quickly set up your datalogger and begin data logging ea F Download the txt file that pertains to your datalogger series see links below Upload that file to your Campbell Datalogger Run the program using your Campbell Datalogger Enable the Data Output Ready Mode on your C3 this will allow streaming of ASCII data at the set sample interval Plug your C3 into the datalogger and watch data stream or capture data using your datalogger Download Firmware on the Turner Designs website http www turnerdesigns com customer care software a firmware NOTE Be sure to use the Integration Cable or Integration Interface Adapter Cable to plug the C3 Submersible Fluorometer into your datalogger using the Standard Interface Cable may result in the loss of data or inability to communicate with your datalogger Be sure to refer to the power requirements in Appendix A under or over power to the instrument may result in damage and loss of data 998 2300 Rev J Page 50 C3 Submersible Fluorometer Appendix O Optical Specification Guide Application l Dynamic Range CWL Excitation Emission eR Std 0 15 15 ppb 0 1250 1250 ppb 2300 251 CDOM 0 5 ppb 0 5000 ppb 365 nm 325 120 nm 470 60 nm 2300 902 2300 200 0 025 ug L 0 500 ug L 465 170 nm_ 696 44nm 23
23. ct will be dealt with per Turner Designs end of life recycling program in an environmentally friendly way C3 Submersible Fluorometer 1 Introduction 1 1 The C3 Submersible Fluorometer is manufactured according to users optical specifications with one two or three optical sensors ranging in wavelengths from deep ultraviolet to infrared Each optical sensor is designed with fixed excitation and emission filters The C3 Submersible Fluorometer comes with a factory installed temperature probe and can be configured with a pressure sensor for depth measurements up to 600 meters and or a wiper to minimize biofouling The C3 Submersible Fluorometer Windows based user interface allows for easy calibration of each sensor digital data reporting data logging and digital or analog export of data 1 2 Important Note Regarding Use of Fluorescein Optics For C3 s manufactured prior to 2 15 2011 the Fluorescein sensor should not be enabled for simultaneous data collection with Chlorophyll and or Rhodamine sensors or it will produce unreliable results For C3 s manufactured after 2 15 2011 this interference issue was corrected If you would like to send your C3 back to Turner Designs to resolve this interference issue please contact sales turnerdesigns com 998 2300 Rev K Page 4 C3 Submersible Fluorometer 2 Inspection and Setup 2 1 Instrument Checklist The C3 Submersible Fluorometer package P N 2300 000 includes C3 Submersi
24. e following steps 1 Write email or call the Turner Designs Technical Support department and describe as precisely as possible the nature of the problem Phone 1 877 316 8049 Email support turnerdesigns com 2 Carry out any adjustments or tests as suggested by the Technical Support Department 3 If proper performance is not obtained you will be issued a Return Materials Authorization number RMA to reference Package the unit write the RMA number on the outside of the shipping carton and ship the instrument prepaid to Turner Designs If the failure is covered under the warranty terms the instrument will be repaired and returned free of charge for all customers in the contiguous continental United States For customers outside of the contiguous continental United States who purchased equipment from one of our authorized distributors contact the distributor If you purchased directly contact us We will repair the instrument at no charge Customer pays for shipping duties and documentation to Turner Designs Turner Designs pays for return shipment custom duties taxes and fees are the responsibility of the customer 4 4 Out of Warranty Service Follow steps for Warranty Service as listed above If our Technical Support department can assist you by phone or correspondence we will be glad to at no charge Repair service will be billed on a fixed price basis plus any applicable duties and or taxes Shipment to Turner Desig
25. e screw Turning clockwise increases the signal and counterclockwise decreases the signal Once the desired reading has been obtained the locking nut should be screwed down so that the adjustment screw is held firmly in place Note The response of every Solid Standard is unique A new correlation must be determined for every sensor Use of the Solid Secondary Standard for in vivo Chlorophyll Applications 1 Using your C3 Submersible Fluorometer measure a sample containing algae and record the response values for that measurement 2 Dry off the optical end of the C3 attach the Solid Secondary Standard Cap and insert P N 2300 901 to the fluorometer and adjust the Solid Secondary Standard to produce the same response as in step 1 3 Perform a chlorophyll extraction to determine the actual chlorophyll concentration of the sample 998 2300 NOTE EPA Method 445 0 in vitro determination of chlorophyll in algae can be found on Turner Designs website 4 The Solid Secondary Standard s signal is now equivalent to the concentration value determined from step 3 and can be used in place of a liquid primary standard for future calibration of that specific C3 Submersible Fluorometer Use of the Solid Secondary Standard for Dye Tracing Applications The Solid Secondary Standard can also be used to check fluorometer stability when making dye concentration measurements If necessary the Solid Secondary Standard
26. ed Geodatabase Draw Edit vertices Editor Effects Feature Cache Feature Construction GPS Toolbar gt ae Geocoding Tools Topology Tracking Analyst C3 Toolbar Turner Designs C3 Utility Network Analyst Versioning Customize Rev K Page 32 C3 Submersible Fluorometer e Connect to GPS on GPS toolbar See ArcMap help for information on this alo x SE Fia a tn Hi Gack Poma pira Flanounce Center Coeseres Favesgas Seach I Adding the GPS toolbar arohls 10 Before working with GS devices within archap you must first add the GPS toolbar a R rs Ah one PRT GPS toolbar buttons and thelr functions Open Connection Opens a GPS connection tor updates tram GPS kzi ih Cleee Connmechon Gages the 25 connecien be shop updates from OS amp Start Streaming Ta Log Stats reami OFS data mto the selected lag A Stop Seeding To Log Stops Sradenng GPS dyta mto the selected Img a tamp Curent Postion To Log Stamps the courront position bo the bog amp Fan To GPS Position Pans the active wew to display the cument GPS position A Zoom To GPS Posten Zoom te the current GPS poston P Ad Degimai Sets the chched powt on the streen as the degirabon GPE neolier piii ad Aaieticcur Steps 1 Clek Customize on the Man menu point to TeHbars and click GPS The toolbar ce oeiched Tip To de the GPS bonbar ight click any bombar and click GPS Related Topic
27. efined Oils and Fuels Deep Ultra Violet No Color Crude Oil Ultra Violet No Color Optical Brighteners Ultra Violet No Color PTSA Ultra Violet No Color Do not look directly at the optics Ultraviolet light can be damaging to the eyes 998 2300 Rev K Page 8 C3 Submersible Fluorometer 3 C Soft Windows User Interface The C Soft Windows based user interface allows for intuitive calibration data logging set up and download functions 3 1 Software Requirements and PC Installation 3 1 1 Minimum PC Requirements 133MHz microprocessor 600 X 800 VGA resolution Microsoft Windows 98 32 MB RAM 3 1 2 Software Installation 1 2 Exit all Windows programs Insert the C Soft CD also located on the Turner Designs Software Firmware webpage under Customer Care Open the C Soft software folder and double click on the setup icon The software will automatically be installed in the PC s C Program Files C Soft folder unless user selects alternate location After restarting the PC the C Soft icon is located on the desktop Gee C 5oft C Soft Note You may receive a warning message asking if you want to unblock messages select Unblock 3 1 3 Integration Interface Adapter Cable Installation 1 998 2300 Insert the C3 software CD and install the Adapter Cable driver Integration Interface_ Adapter _Drivers exe onto your PC Make sure nothing is plugged into the Adap
28. er Note Use the Battery Life Calculator available on the Turner Designs website C3 product page to calculate a conservative estimation of how many days you can expect to log data Factors such as battery charge and water temperature will affect logging days A few seconds after the Yes button has been clicked to enable the Data Output Ready State the C Soft program will automatically shut down and users must disconnect power and serial connections The instrument is now in the Data Output Ready State and will remain in that state so long as C Soft is not used to communicate with the instrument Note As soon as C Soft is used to communicate with the instrument users will need to re set the parameter settings in the Log Setup section 3 4 and again enable datalog to put the instrument into the Data Output Ready State When power is supplied to the instrument while it is in the Data Output Ready State it will begin streaming data after a 100 second delay If power is cycled i e disconnected and reconnected please allow 100 seconds for the data stream to begin Note Check the optical face when power is supplied to make sure the sensors light The sensor will turn on then off and then turn back on in 100 seconds If this sequence does not happen cycle power 3 6 Downloading and File Management Users can download new and existing files saved in the C3 Submersible Fluorometer memory Select Download Data Note This step may t
29. er to the C3 12 VDC Power Supply Car Adaptor P N 2900 151 This is an optional accessory for the C3 This cable connects to the power connector on the interface or integration cable and a car cigarette lighter receptacle or other similar receptacle 998 2300 Rev K Page 28 C3 Submersible Fluorometer 24 Pigtail Interface Cable with Locking Sleeve P N 2200 170 This cable is an optional accessory for the C3 It can be used to connect the C3 to a datalogger and or external power source that the user must wire themselves replacing the standard interface and integration cables Extra care should be taken with this cable to make sure all wiring specifications are strictly followed i a eo em Power ground and V BATT are not common Battery Interface Cable with Locking Sleeves P N 105 2590 This cable is an optional accessory for the C3 Itis intended for deployments in stand alone mode Note When using this cable to connect to the battery the battery bracket is not required Use of the battery bracket is recommended when possible as it provides stability for the connection Extender Cables 10 Meter P N 105 2595 shown 25 Meter P N 105 2596 requires P N 2200 900 50 Meter P N 105 2597 requires P N 2200 900 Custom lengths are available upon request These cables are optional accessories for the C3 They allow the C3 to be deployed at a variety of depths 998 2300 Rev K C3 Subm
30. ersible Fluorometer Boosters P N 2200 900 The Booster Kit is an optional accessory for the C3 Connect to extender cable P N 105 2596 or 105 2597 and is required for cable lengths greater than 10 Connects to power source cable meters The Boosters supply a stable power source ee aa and maintain connection with the C3 for deployment depths greater than 10 meters The simple and rugged design allows the Boosters to be used in any aquatic environment sa Connects to instrument Pigtail Cable with Locking Sleeve 2 foot Pigtail Cable with Locking Sleeve P N 2100 750 shown 5 Meter Pigtail Cable with Locking Sleeve P N 2100 755 10 Meter Pigtail Cable with Locking Sleeve P N 2100 751 25 Meter Pigtail Cable with Locking Sleeve P N 2100 752 50 Meter Pigtail Cable with Locking Sleeve P N 2100 753 These cables are required for use with the Analog Adapter P N 2300 480 They connect to the Analog Adapter s 6 pin male end and have six bare wire leads to be used for integrating with CTDs or third party dataloggers Function V BATT V BATT repo TH Green Araog O75 WO Analog Adapter P N 2300 480 This is an optional accessory and allows users to convert the standard digital output from the C3 Submersible Fluorometer to a 0 5 VDC analog signal The Adapter can convert the signal from 1 2 or 3 channels installed on a C3 998 2300 Rev K Page 30 C3 Submersible Fluorometer
31. green support line from the base cap of the C3 Submersible Fluorometer Insert green line provided to help remove installed l green line 5 Slide the submersible battery pack through the bracket towards the male connector on the C3 s 6 Mate the 8 pin male connector on the C3 with the female connector on the battery pack 2 Pull the base cap off of the C3 Submersible Fluorometer 7 Secure the battery connection using the Allen Hex Key provided to tighten the Hex 3 Position the Battery Bracket with the C3 Screw located on the bracket s locking so the alignment strips line up with one ring around the battery another and the bracket s alignment pin fits into the C3 s alignment hole Battery Bracket locking ys file Alignment Fully mated Allen Hex Screw Battery Strips battery pack a eee base 998 2300 Rev K Page 36 C3 Submersible Fluorometer Appendix G Charging the Submersible Battery Pack Introduction The Submersible Battery Pack P N 2200 601 contains a rechargeable lithium ion battery and battery pack charger The battery pack is used together with the Internal Datalogger to enable self contained use of the C3 Submersible Fluorometer The battery pack has a 600m depth rating and facilitates vertical profiling and moored applications Charging the Battery 1 Carefully align the battery s connector to the pins of the charger and push the battery straight onto the charger 2 Pl
32. ll Rhodamine WT Fluorescein Phycocyanin and Phycoerythrin P N 2100 902 Solid Secondary Standard insert for CDOM Crude Oil Refined Fuels and Optical Brighteners Solid Secondary Standard Cap numbers Features Eaa 2 Slightly rotate cap until it is set into e Can be used in place of a primary liquid position Note You will feel hear a click standard once a correlation between a when cap is positioned primary standard and the solid standard is established 3 Insert up to 3 Solid Standards into the desired optical ports on the Solid e Can be used to check fluorometer stability Standard Cap and or check for loss in sensitivity e Provides a broad range of very stable fluorescent responses e Has an adjustment screw allowing users to set to a desired signal Numbered Positioning Positions Screws 4 Rotate the Solid Standard s until set into position Note You will feel hear a click Adjustable Locking when the Solid Standard is positioned Screw 998 2300 Rev J Page 41 C3 Submersible Fluorometer Solid Standard Adjustment To adjust each solid standard use a screwdriver to unscrew the locking nut as far as it will go Adjustable Locking Screw To change the signal level insert the green screwdriver through the hole in the locking nut Rotate it until it engages with the adjustment screw that is beneath the locking nut The signal can be adjusted to the desired reading by turning th
33. log Adapter converts digital data to voltage to help users integrate our in situ fluorometers with almost any CTD third party system or data logger that accepts a 0 5 volt signal Below are two ways to interpret voltage output based on the C3 s setup 1 Reading the Voltage Output for RFUB Calibrated C3 s The voltage output from the C3 Submersible Fluorometer via the Analog Adapter needs to be blank corrected Users can standardize the voltage signal using a standard of known concentration to convert RFUB to direct concentrations 2 Reading the Voltage Output for Specific Range Calibrated C3 s The voltage output from the C3 Submersible Fluorometer via the Analog Adapter needs to be blank corrected The voltage output reflects direct concentrations re scaled to 0 5 volts Actual Concentration blank subtracted voltage 5 volts x range maximum at 5 volts Example if your measured voltage for a given sample is 2 567 volts and the blank voltage is 0 067 volts your blank subtracted voltage is 2 5 volts Actual Concentration 2 567 volts 0 067 volts 5 volts x range maximum at 5 volts If your range maximum equals 100 ug L then 0 5 100 ug L 50 ug L Actual Concentration 2 5 volts 5 volts x 100ug L at 5 volts Actual Concentration 0 5 volts x 100 ug L at 5 volts 50 ug L Therefore a sample that measures 2 567 volts 50 ug L 998 2300 Rev J Page 45 C3 Submersible Fluoromete
34. ngs menu for that USB Serial Port Choose any COM port 1 6 from the COM port pull down menu and click OK Note Ideally you want to start with any port that is not in use if all are in use then choose any port 1 6 Click OK to the warning box if it appears and Click OK to close any other open menus Your installation is complete Make sure to set C Soft to that specified COM designation when trying to connect via USB Adapter Cable connection 3 1 4 PC Software Interface Note It is important that the steps be followed INORDER Any variance from the procedure may result in the connection NOT being established 1 Plug either the USB or serial connector of the adapter cable into your computer Note Either connection can be used for communicating and configuring the instrument 2 Plug the interface cable onto the 8 pin bulkhead connector of the instrument 3 Plug the interface cable s serial plug into the adapter cable s male serial plug 4 Begin C Soft by double clicking on the C Soft icon and choose the appropriate COM port from the pull down COM menu Plug the 12V power supply into a power source 6 Wait for initialization process to complete i x Initializing Unit 998 2300 Rev K Page 10 C3 Submersible Fluorometer The connection icon located on the upper right of the screen will turn green if connected Note If the connection icon does not turn green disconnect and reconnect power C
35. ns should be prepaid Your bill will include return shipment freight charges Address for Shipment Turner Designs Inc 845 W Maude Ave Sunnyvale CA 94085 998 2300 Rev K Page 23 C3 Submersible Fluorometer 998 2300 Rev K Page 24 C3 Submersible Fluorometer Appendix A Specifications C3 Submersible Fluorometer Weight in Air 1 64 kg 3 6 Ibs 3 Temperature Accuracy 0 5 0 1 Signal Output Digital ASCII or Analog Note analog requires accessory Interface RS232 USB Adapter Nonvolatile Memory 64 000 Data Lines Submersible Battery Pack Voltage Output 14 8V nominal Protects against overcharge over discharge overheating Protection Features cd short circuiting 998 2300 Rev K Page 25 C3 Submersible Fluorometer Appendix B Recommended Practices Identification Sensors are labeled 1 2 or 3 adjacent to the optics on the face of the instrument Each channel is factory set in the C3 Windows Software Note The channel labels can be modified in the C Soft software Recommended Measurement Practices The following steps will improve the accuracy and repeatability of your measurements especially at low concentration levels 1 Use a dark plastic or glass container for your standards and or samples Note Some plastics may fluoresce and might interfere with measurements If using a glass beaker place it on a non reflective surface preferably black Ensure that the sensor is gt 3 inches
36. olid Secondary Standard P N 2300 902 to ensure that it is not exposed to UV light for prolonged periods of time This can result in degradation of the standard Ordering Information Part Number 2300 905 2300 901 2300 902 OBSOLETE 2300 900 Solid Secondary Standards are not available for Turbidity sensors 998 2300 Rev J Page 43 C3 Submersible Fluorometer Appendix K Analog Output Adapter Introduction The Analog Output Adapter P N 2300 480 allows users to convert the standard digital output from the C3 Submersible Fluorometer to a 0 5 VDC analog signal The Adapter can convert the signal from 1 2 or 3 channels installed on a C3 The voltage output is proportional to the fluorescence intensity measured by the sensors Modeled after the C3 Submersible Fluorometer s battery pack the Analog Adapter is designed to connect directly to the C3 The C3 Battery Bracket P N 2300 603 is required to hold the Analog Adapter in place and secure it preventing any break in communication or power With the Analog Adapter users can integrate their C3 Submersible Fluorometers into any CTD or multi parameter platform that will accept a 0 5 volt output signal Required Accessories The following accessories are required for installation and operation of the Analog Adapter Battery Bracket PN 2300 603 Designed to hold the Analog Adapter in place and support it to avoid any break in communication or power ensuring continuou
37. ove the spacer and the brush Slide in a new brush Slide in the spacer Position the brush arm onto the wiper assembly and tighten the arm locking screw Page 38 C3 Submersible Fluorometer Appendix Installing the Flow Cap Installation Introduction The Flow Cap P N 2300 700 for the C3 Submersible Fluorometer enables flow through mode The Flow Cap will allow the C3 Submersible Fluorometer to be configured with other instruments used in flow through systems The C3 Flow Cap e Eliminates other light sources that may interfere with sensor measurements e Protects sensors from damage during deployment or transport e Helps to limit bio fouling The Flow Cap can be installed on C3 Submersible Fluorometers with or without the mechanical wiper The Flow Cap can also be used as a calibration cup for C3 sensors using standard calibration solutions See following page for calibration cup use Flow Cap Housing N Threaded ends for easy installation and removal End Cap we LF 4 SY NOTE The End Cap comes with an installed O Ring that should not be removed or sealing will be compromised 998 2300 1 Turning clockwise fully hand tighten the Flow Cap housing onto the C3 Submersible Fluorometer The port located on the Flow Cap Housing should be closest to the optical head 2 Turning clockwise hand tighten the End Cap onto the Flow Cap Housing until it is fully tightened 3
38. r Wiring Guide The wiring guide below indicates the function of each pin for the Analog Adapter s 6 pin male end To avoid damaging the Analog Adapter please ensure the pins are only used for their intended purpose Analog Adapter s 6 pin male end Analog Out 1 0 5 VDC Analog Out 2 0 5 6 CONTACT VDC Analog Out 3 0 5 VDC Analog GND 998 2300 Rev J Page 46 C3 Submersible Fluorometer Appendix L How to set up Hyperterminal for Digital ASCII Data Capture 1 Ensure C Soft program is not running in the background On an MS Windows computer open the HyperTerminal program Start All Programs Accessories Communications HyperTerminal 2 The Connection Description screen will appear Name the connection description This file will save the communication parameters for the C3 Submersible Fluorometer and can be used in the future to establish communication with the instrument quickly 3 The Connect To screen will appear Choose the appropriate communications port in the Connect Using window Click OK 4 The Port Settings screen will appear Choose the following port settings Bits per second 9600 2 x Data bits 8 Port Settings Parity None Stop bits 1 Bits per second EANN Flow control Hardware E ata bits Click Apply and OK Parity None Stop bits fi Flow control Hardware Restore Defaults Cancel Apply 5 Next the HyperTerminal window will appea
39. r You are now ready to connect the C3 Submersible Fluorometer to a power source Data will be displayed at set intervals in the HyperTerminal window after 100 seconds or at the specified log start time C Soft HyperTerminal Ss loj x File Edit View Call Transfer Help Dle a3 RhodaminePhycocyani Depth Temp C 98 65 129 70 0 00 23 71 Date Time 6 16 08 15 07 24 6 16 08 15 07 29 01 09 130 71 0 00 23 72 6 16 08 15 07 26 01 61 130 83 0 00 23 72 6 16 08 15 07 27 01 23 130 95 0 00 23 72 6 16708 15 07 28 91 09 130 95 0 00 23 72 6 16 08 15 07 29 01 61 130 92 0 00 23 72 6 16 08 15 07 30 31 23 131 10 0 00 23 72 998 2300 Rev J Page 47 C3 Submersible Fluorometer Most applications will integrate streaming data Below are instructions on how to save streaming data on a PC To save data 1 On the HyperTerminal window toolbar choose Transfer and then Capture Text File Edit View Call Transfer Help alz af Send File Receive File Capture Text Send Text File ae Capture to Printer 2 When you click on Capture Text a window will appear asking you to name and save the txt file to the location of interest Folder C Documents and File SEA TURNERDESIGNSSCAFPTURE TST Browse ea 3 Once you are finished you will need to return to the HyperTerminal window toolbar choose Transfer and then Capture Text and select Stop This will stop data capture to the txt file File
40. rs in either the direct concentration mode raw fluorescence mode or blank subtracted raw fluorescence mode nix Unit Labed crem 1 Rhorkene Orrin LRU 93 20 Step 1 Press Set Blank to sat the blank Blink RAU value to the qurent RFU xt eat ear Step 2 Enter the valve of your standard Standard Value nel eect Eh Lats ot reine Sten 3 Pres Set Siaina to ant the standard RFL standard value to the curent Sak Standard RAJ ep4 Optional Oke the temperature Tame Compercation compensation bow to activate E Step I After Calibration e complete press Seve Calibration eve Colla al Cancel Chick tous uncalibrated Rae Phucrescernce Mode Direct Concentration Mode Values reported in the Direct Concentration Mode are scaled to a predetermined standard value and blank subtracted The equation used to calculate concentrations after calibrating to Direct Concentration Mode IS Standard Value units x Sample RFU Concentration units Standard RFU Raw Fluorescence Blank Subtracted Mode Values in the Raw Fluorescence Blank Subtracted Mode are noted as RFUB It is important to note that these values are blank subtracted but are not scaled to a standard they are relative values Raw Fluorescence Mode Values in the Raw Fluorescence Mode are referred to as Relative Fluorescence Units RFU It is important to note that these values are not blank subtracted or scaled to a standard they are relative values 99
41. s x E e Set up C3 Connection using C3 Toolbar Setup C3 z E 7 TurnerDesignsC3 0 FNI Ss Setup C3 52 Show Live C3 Data gt oj P e Choose Com Port and Channel Settings then hit connect button Data should start streaming in as shown below C3ComPot COM4 gt C3Staus FOtOpen aus C3 Data Valid Enable Mame Units Value Chan 1 Rhodamine PPE 8 20 Chan 2 M Turbidity NTU 11 92 Chan 3 Iw Chlorophyl a a7 7 16 Date Time Depth m Temp C fiv220 10 06 55 4 66 19 12 _5etLog Fie i STE set Eonnect Disconnect 3 Log Interval Seconds e Choose Shapefile to log to using Set Log File Button form can now be closed 998 2300 Rev K Page 33 C3 Submersible Fluorometer e Open Show Live C3 Data form which shows larger version of C3 data along with GPS data and Port Logging Status Depth rn Channel 1 Porn Open C3 Status C3 Data Yalid 9 66 0 92 Log Status Not Logging Temp Channel 2 Log File 19 16 11 16 Latitude DD ddd Time Channel 3 41 25876 10 11 03 6 64 EE va pala e Start stop logging to file using toolbar buttons Log Data To File Starts Logging to file based on Log interval Stop Pause Logging to File Stops Pauses logging Fiaa Data Point Adds Flag to current data point and gets user notes on flag e g Bad reading C3 out of water or Start Transect 3 Flag Notes Add Flag Notes x _C
42. s data output An Analog Pigtail Cable We offer various lengths that are necessary for standard integrations P N 2100 750 2 foot pigtail cable P N 2100 755 5 meter pigtail cable P N 2100 751 10 meter pigtail cable P N 2100 752 25 meter pigtail cable P N 2100 753 50 meter pigtail cable C3 Submersible Fluorometer Setup There are only a few steps required to set up the C3 Submersible Fluorometer for analog output There are two methods that may be used for calibrating C3 channels sensors The first is a Raw Fluorescence Units Blanked Calibration refer to section 3 3 2 in the C3 s user manual and the second is a Direct Concentration Calibration refer to section 3 3 1 in the C3 s user manual Use the Raw Fluorescence Units Blanked RFUB calibration if you do not have a standard with known concentration or if you are unsure what numeric value to assign your standard NOTE Repeat either one of the above calibrations for each channel that will be used for Analog Output 998 2300 Rev J Page 44 C3 Submersible Fluorometer Once calibration is complete click the Log Setup tab to set the logging start time amp logging interval Click enable datalog wait for C Soft to automatically shut down and disconnect power As soon as power is re supplied to the C3 via the Analog Adapter it will automatically begin data output after 90 seconds or pre configured start time at the set interval Analog Output The Ana
43. sible Fluorometer Appendix H Introduction 7 The Mechanical Wiper P N 2300 450 for the C3 Submersible Fluorometer is designed to reduce bio fouling during extended deployments Benefits e Helps maintain consistent readings by reducing bio fouling e Replacement brushes available P N 2300 460 Installation 1 Connect the C3 to a computer that has C Soft software and supply power 2 Start the C Soft software 3 Click on the Log Data tab 4 Click the Test Wiper button to bring the motor shaft to its home position 5 Install O ring into motor shaft groove Note Do not lubricate O ring 6 Slide the wiper assembly onto the motor shaft with brushes facing towards the optical head and position the brushes between two sensors St O ring Motor shaft 1 Tightening nut 2 3 4 5 Sensors 998 2300 Rev J Installing the Mechanical Wiper Hand tighten nut to secure wiper assembly Note do not use any tools to tighten the tightening nut Tightening nut Click Test Wiper If the wiper does not come to rest in the desired position unscrew the tightening nut remove the wiper assembly and repeat steps 6 8 Replacement Brush Installation a k Wiper hub Ss N Spacer f Brush Arm locking screw pr Brush arm Using the Allen Wrench provided loosen the arm locking screw Pull out the brush arm with brush Slide the brush towards the open end of the brush arm to rem
44. t will appear asking if user is ready to enable logging select Yes Note If data logging is set to start for a time in the past there will be a minimum delay of 100 seconds before startup after power has been supplied Otherwise wait until the data logging start time has been reached for data logging to begin WARNING As soon as data logging is enabled users will be unable to access C Soft settings This should be the last step before the C3 Submersible Fluorometer is deployed 3 5 Enabling Data Output Ready State The Data Output Ready State must be enabled for the C3 Submersible Fluorometer in order to log data capture real time data output and or integrate the C3 with other instrumentation see Appendix M and N for more information about integration There are five necessary steps to ensure the instrument is properly set up for data output 1 Set the instrument s clock to PC or local time in the Settings Tab 2 Select the sensors you expect to use during sampling 3 Calibrate selected sensors for either Direct Concentration Mode or Raw Fluorescence Units Blanked RFUB Mode see section 3 3 4 Set the start time sampling and wiping intervals in the Log Setup Tab Note Wiping interval is optional and depends on the sampling interval see section 3 4 5 Click the Enable Datalog button followed by the Yes button to enable the Data Output Ready State 998 2300 Rev K Page 19 C3 Submersible Fluoromet
45. ter Cable s connectors Plug the USB connector into a USB port on your computer and wait for hardware to automatically install Once USB is installed and ready to use right click the My Computer Icon Note For Windows Vista or 7 users open the start menu and right click on Computer Left click properties to open the properties window If running Windows XP or older versions of Windows click on the Hardware tab and then click Device Manager to open the list of devices installed on your computer Note For Windows Vista or 7 users the Device Manager link Is located in the upper left part of the properties window you will not see a Hardware tab Rev K Page 9 C3 Submersible Fluorometer Scroll down and open the Ports heading from the list The newly installed Adapter will be titled USB Serial Port followed by a COM port designation in parentheses for example COM 5 9 If the USB Serial Ports COM designation is labeled as any number from 1 6 your installation is complete Close all windows and make sure to set C Soft to that specified COM designation when trying to connect via USB Adapter Cable connection 10 If the USB Serial Port s COM designation is greater than 6 right click on the USB Serial Port and click properties which will open up the properties menu From that menu click on the Port Setting tab and then click Advanced which will open the advanced setti
46. ttery Bracket and Battery Charging the Submersible Battery Pack Installing the Mechanical Wiper Installing the Flow Cap Using the Solid Secondary Standard C Series Analog Adapter How to set up Hyperterminal for Digital ASCII Data Capture Integrator Firmware Campbell Scientific Datalogger Integration Optical Specification Guide OZSEZr Alc TATMIMVOAWS C3 Submersible Fluorometer WASTE ELECTRICAL AND ELECTRONIC EQUIPMENT WEEE DIRECTIVE Turner Designs is in the business of designing and selling products that benefit the well being of our environment Accordingly we are concerned with preserving the surroundings wherever our instruments are used and happy to work with customers by complying with the WEEE Directive to reduce the environmental impact resulting from the use of our products WEEE Return Process To arrange the return of an end of life product proceed as follows If you purchased your instrument through a Turner Designs Distributor please contact your local representative They will instruct you where to return the end of life product If you purchased your instrument directly from Turner Designs please contact Turner Designs Customer Service By Phone 1 408 212 4041 or Toll Free 877 316 8049 By Email Customer Service at support turnerdesigns com Turner Designs will provide a WEEE RMA Number a Shipping Account Number and a Ship to Address Package and ship the product back to Turner Designs The produ
47. ug the charger into a standard wall outlet and allow the battery to charge for 6 hours for a complete battery charge 3 When finished unplug the charger and remove the battery by pulling the battery straight out from the charger s connector Battery s Connector Charger pin General Precautions Under no circumstances should you attempt to open the battery housing Please contact Turner Designs if you have any problems or questions related to the battery pack 998 2300 Battery Life The battery is designed when fully charged to exceed the Internal Data Logging capacity at the maximum sampling rate For longer deployments at shorter sampling intervals we recommend recharging the battery after the Internal Datalogger is full or every 3 4 months whichever comes first Note Use the Battery Life Calculator available on the Turner Designs website C3 product page to calculate a conservative estimation of how many days you can expect to log data Factors such as battery charge and water temperature will affect logging days Specifications 9 3 X 2 75 Dimensions 13 46cm x 7 0cm Weight in Air 1 65lbs 0 75kg Weight in Water 0 50Ibs 0 22kg Rated Depth 600m 1969 feet Voltage Output 14 8V nominal Battery Type Number of Cells Protects against Protection Overcharge Over Features Discharge Overheating Short Circuiting Charge with Turner Designs charger only Page 37 C3 Submer
48. with latitude as designated from the UNESCO Technical Papers in Marine Science 44 http unesdoc unesco org images 0005 000598 059832EB pdf This method assumes 0 C and 35PSU salinity So lolx Channel Calibration Unit Label Depth Current Pressure 0 00 PSI Step 1 Press Set Zero to set the Zero Reading atmospheric zero Set Zero Step 2 Select a pressure compenstion iS Atmospheric offset only method Atmospheric and gravity offset Step 3 When you are done press Save Calibration or press Cancel at Save Calibration Cancel any time to abort this calibration Atmospheric Offset Method 1 Step 1 Select the Calibrate button for depth in the Current Data screen Pressure in PSI will be displayed in the Current Pressure window After Current Pressure readings are stable at desired location select Set Zero Step 2 Select Atmospheric offset only Step 3 Select Save Calibration button Depth values will now be scaled to the offset selected Rev K Page 17 C3 Submersible Fluorometer Atmospheric and Gravity Offset Method 2 Step 1 Select the Calibrate button for depth in the Current Data screen Pressure in PSI will be displayed in the Current Pressure window After Current Pressure readings are stable at desired location select Set Zero Step 2 Select Atmospheric
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