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Agilent 1290 Infinity Thermostatted Column Compartment User

1. Initial firmware A 06 10 main and resident Compatible with All other modules must have firmware revision A 06 1x or B 06 1x or 1100 1200 series above main and resident Otherwise the communication will not work modules Conversion to N A emulation 1290 Infinity TCC User Manual 143 8 Maintenance Replacing the Module Firmware 144 1290 Infinity TCC User Manual 1290 Infinity TCC User Manual 9 Parts and Materials for Maintenance Plastic Parts 146 Leak Parts 147 Heater and Cooling Devices 148 Valve Options Overview 152 Accessory Kits 155 Standard Accessory Kit 155 Low Dispersion Capillary Kit for G1316C 156 Accessories 162 This chapter provides information on parts for maintenance Apg Agilent Technologies 145 9 Parts and Materials for Maintenance Plastic Parts Plastic Parts Item p n Description 1 G1316 68754 Front cover 2 5042 9964 Name plate for Agilent 1290 series 2 Figure 38 Plastic Parts 146 1290 Infinity TCC User Manual Parts and Materials for Maintenance 9 Leak Parts Leak Parts Item p n Description 1 G1316 67000 Leak tube kit includes following items Funnel holder G1316C tubing flex polyethylene leak funnel 2 61316 42303 Capillary guide Figure 39 Leak Parts 1290 Infinity TCC User Manual 147 9 Parts and Materials for Maintenance Heater and Cooling Devices The use of these heater devices is described in Agilent 1200 Series Multi Method and Method D
2. oe Agilent Technologies Notices Agilent Technologies Inc 2008 2009 2011 2012 2013 No part of this manual may be reproduced in any form or by any means including elec tronic storage and retrieval or translation into a foreign language without prior agree ment and written consent from Agilent Technologies Inc as governed by United States and international copyright laws Manual Part Number 61316 90032 Edition 02 2013 Printed in Germany Agilent Technologies Hewlett Packard Strasse 8 76337 Waldbronn This product may be used as a com ponent of an in vitro diagnostic sys tem if the system is registered with the appropriate authorities and com plies with the relevant regulations Otherwise it is intended only for gen eral laboratory use Warranty The material contained in this docu ment is provided as is and is sub ject to being changed without notice in future editions Further to the max imum extent permitted by applicable law Agilent disclaims all warranties either express or implied with regard to this manual and any information contained herein including but not limited to the implied warranties of merchantability and fitness for a par ticular purpose Agilent shall not be liable for errors or for incidental or consequential damages in connection with the furnishing use or perfor mance of this document or of any information contained herein Should Agilent and the use
3. Probable cause Suggested actions 1 Defective heater assembly Please contact your Agilent service representative 2 Defective TCC board Please contact your Agilent service representative Defective Heater Circuit Error ID 2860 The electronic circuit for control of the heater assemblies is defective The processor checks the function of the heater circuits continually If a defect is detected in the control circuit the processor switches OFF the heater peltier assemblies and the error message is generated Probable cause Suggested actions 1 Defective TCC board Please contact your Agilent service representative 1290 Infinity TCC User Manual 99 6 Error Information Valve failed to initialize Error ID 2875 During initialization the valve actuator turns until the encoder reads the reference index position An error is generated if the reference index cannot be found within a given time Probable cause Suggested actions 1 Defect in cable connection of valve drive Please contact your Agilent service control representative 2 Defect in cable connection of valve actuator Please contact your Agilent service encoder reader representative 3 Defect of valve drive or valve actuator Please contact your Agilent service encoder reader representative Unknown Unsupported Valve detected Error ID 2872 After powering on the RFID tag of the valve head is read out An error is generated if the RFID tag does not contain
4. 1290 Infinity TCC User Manual Site Requirements and Specifications 2 Table 17 G5632A Bio inert 2pos 10port Valve Head Type Specification Liquid contacts Port size Flow passage diameters Port to Port Volume Maximum pressure PEEK Ceramic Accepts 10 32 male threaded fittings 0 38 mm 0 015 in Stator 0 30 mm 0 012 in Rotor Seal 1 71 uL 600 bar Table 18 G5639A Bio inert 4 Column Selector Type Specification Liquid contacts Port size Flow passage diameters Port to Port Volume Maximum pressure PEEK Ceramic Accepts 10 32 male threaded fittings 0 25 mm 0 010 in 0 51 uL pre column volume 1 33 uL post column volume 600 bar 1290 Infinity TCC User Manual 33 2 Site Requirements and Specifications Extended Specifications on G1316C L shaped pre column heater Heater long up L shaped pre column heater Heater long down 34 The 1290 Infinity Thermostatted Column Compartment G1316C is usable from 10 C below ambient up to 80 C for flow ranges up to 5 ml min and up to 100 C for flow ranges up to 2 5 ml min Additional heating devices are available for the G1316C to reduce the risk of additional dispersion at low flow rates see Figure 8 on page 34 These devices can be installed in any position in the column compartment see Availability of heater devices on page 56 L shaped pre column heater Volume 1 6 pl
5. Figure 40 TCC Heater Fittings 150 1290 Infinity TCC User Manual Parts and Materials for Maintenance 9 Heater and Cooling Devices sac HK ie Y g EO T ES Holes to attach carrier L shaped pre column heater volume 1 6 pL mounted on carrier top for column 1 bottom for column 2 Figure 41 Heater Devices for G1316C 1290 Infinity TCC User Manual 151 9 Parts and Materials for Maintenance Valve Options Overview Valve Head Parts The figure below illustrates replacement parts for the valve heads with the 12Pos 13Port aiuim Selector valve as an example The valves can vary in their appearance and do not necessarily include all of the illustrated parts Neither every spare part is available for each flavor of the valve Use the tables Table 24 on page 153 Table 25 on page 154 for identification of the required part numbers oF Figure 4 Valve Head Parts example shows a 5067 4159 valve head Stator screws Stator head assembly Stator face assembly Stator ring available for service only Rotor seal Bearing ring P N 1534 4045 Spanner nut P N 5068 0106 available for service only YN oOo 7 FP OO N gt 152 1290 Infinity TCC User Manual Parts and Materials for Maintenance 9 Valve Options Overview This overview gives a summary of the main parts and assemblies More details are available with each valve option in this chapter
6. Install the new isolation seal if required Ensure the metal spring inside the ring faces towards the valve body 7 Install the new rotor seal 134 1290 Infinity TCC User Manual CAUTION Maintenance 8 8 Replace the stator ring Ensure the stator ring is flush with the valve body 9 Place the new if required stator face in place on the stator head Reinstall the stator head 10 Insert the stator screws in the stator head Tighten the screws alternately two turns at a time until the stator head is secure 11 Reconnect the pump capillaries to the valve ports Slide the waste tube into the waste holder in the leak tray Wrong use of Pressure Test may damage valve The current implementation of the Pressure Test automatically uses the maximum pressure generated by the pump used in the system gt Do not use the test for modules having a lower maximum pressure than the pump as this will damage the valve For example do not use 400 bar valve in a TCC or Flex Cube in combination with a 600 bar pump 12 Perform a Pressure Test to ensure the valve is pressure tight 1290 Infinity TCC User Manual 135 8 Maintenance Replacing Valve Heads Several optional valve heads are available which can be installed and exchanged easily Micro valves offer small internal volumes for minimum peak broadening ideal for low flow rates in the nl min and ul min range FM For bio inert modules use bio inert parts only Parts required
7. Table 24 Replacement Parts Standard Valve Heads Valve Head Rotor Seal Stator Head Stator Stator Ring Screws 10 Pack 5067 4107 5067 4111 5068 0001 1535 4857 5068 0120 8 Pos 9 Port 600 bar PEEK 5067 4121 5068 0002 5068 0001 1535 4857 5068 0120 8 Pos 9 Port 1200 bar Vespel 5067 4137 0101 1409 0101 1417 1535 4857 5068 0120 2 Pos 6 Port 600 bar PEEK 5067 4117 5068 0008 5068 0006 1535 4857 5068 0120 2 Pos 6 Port 1200 bar Vespel 5067 4144 0101 1415 0101 1421 5068 0054 n a 2 Pos 10Port 600 bar micro PEEK 5067 4118 5068 0012 5068 0011 5068 0019 n a 2 Pos 10 Port 1200 bar Vespel 5067 4145 0101 1415 5068 0165 5068 0019 n a 2 Pos 10Port 600 bar PEEK 5067 4146 5068 0076 5068 0077 5068 0089 n a 6 Column Selector 600 bar PEEK 5067 4142 5068 0067 5067 0077 5068 0089 n a 6 Column Selector 1200 bar Vespel 1290 Infinity TCC User Manual 153 9 Parts and Materials for Maintenance Table 25 Replacement Parts Bio Inert Valve Heads Valve Head Rotor Seal Stator Head Stator Face Stator Stator Ring Screws 10 Pack 5067 4148 0101 1409 5068 0060 0100 1851 5068 0020 5068 0020 2 Pos 6 Port 600 bar bio inert PEEK 5067 4132 5068 0041 5068 0040 5068 0095 5068 0059 n a 2 Pos 10Port 600 bar bio inert PEEK 5067 4134 5068 0045 5068 0044 5068 0093 5068 0059 n a 4 Column Selector 600 bar bio inert PEEK 5067 4159 0101 1288 5068 0097 0101 1288 5068 0059 n a 12 Pos 13Port Selector 200 bar PEEK bi
8. The serial number information on the instrument labels provide the following information CCXZZ00000 CC ZZ 00000 Format Country of manufacturing DE Germany e JP Japan e CN China Alphabetic character A Z used by manufacturing Alpha numeric code 0 9 A Z where each combination unambiguously denotes a module there can be more than one code for the same module Serial number 1290 Infinity TCC User Manual Hardware Information 11 Electrical Connections Rear view of the module APG Remote RS 232 C Configuration switch Security lever EE Agilent Technologies Cem safety standards configuration switch settings AefeatTochelogins E337 Walibesea CRETE ENT voltage range Made in Germany maser power consumption L frequency Figure 44 Rear view of the module 1290 Infinity TCC User Manual 183 11 Hardware Information Interfaces The Agilent 1200 Infinity Series modules provide the following interfaces Table 26 Agilent 1200 Infinity Series Interfaces Module CAN LAN BCD LAN RS 232 Analog APG Special optional on board Remote Pumps G1310B Iso Pump 2 Yes No Yes 1 Yes G1311B Quat Pump G1311C Quat Pump VL G1312B Bin Pump K1312B Bin Pump Clinical Ed G1312C Bin Pump VL 1376A Cap Pump G2226A Nano Pump G5611A Bio inert Quat Pump G4220A B Bin Pump 2 No Yes Yes No Yes CAN DC OUT for CAN slaves G4204A Quat Pump G1361A Prep
9. gt 2 C minute During the heating phase the temperature change should be gt 3 C minute Defective thermostat components may cause cooling or heating rates to fall outside these limits 1290 Infinity TCC User Manual 105 7 Test Functions and Calibrations Pressure Test For running a Pressure Test please refer to the corresponding pump manual The Pressure Test may be used for testing the tightness of a valve installed in the TCC or Flex Cube CAUTION Wrong use of Pressure Test may damage valve The current implementation of the Pressure Test automatically uses the maximum pressure generated by the pump used in the system gt Do not use the test for modules having a lower maximum pressure than the pump as this will damage the valve For example do not use 400 bar valve in a TCC or Flex Cube in combination with a 600 bar pump 106 1290 Infinity TCC User Manual Test Functions and Calibrations 7 Temperature Calibration Temperature Calibration Principle The actual temperatures of the column heat exchangers left and right depend on the column setpoint temperature For setpoint temperatures above 36 C the heat exchangers are heated to a temperature slightly above the setpoint temperature Conversely for setpoint temperatures below 36 C the heat exchangers are kept at a temperature slightly below the setpoint temperature This fine temperature correction compensates for the small amount of heat exchange through th
10. ott Agilent Technologies 23 2 Site Requirements and Specifications Site Requirements Power Considerations The module power supply has wide ranging capability It accepts any line voltage in the range described in Table 3 on page 27 Consequently there is no voltage selector in the rear of the module There are also no externally accessible fuses because automatic electronic fuses are implemented in the power supply Hazard of electrical shock or damage of your instrumentation can result if the devices are connected to a line voltage higher than specified gt Connect your instrument to the specified line voltage only The module is partially energized when switched off as long as the power cord is plugged in Repair work at the module can lead to personal injuries e g electrical shock when the cover is opened and the module is connected to power gt Always unplug the power cable before opening the cover gt Do not connect the power cable to the instrument while the covers are removed CAUTION Inaccessible power plug In case of emergency it must be possible to disconnect the instrument from the power line at any time gt Make sure the power connector of the instrument can be easily reached and unplugged gt Provide sufficient space behind the power socket of the instrument to unplug the cable 24 1290 Infinity TCC User Manual Site Requirements and Specifications 2 Power Cords Dif
11. 1 0 inches of space on either side and approximately 8 cm 8 1 inches in the rear for air circulation and electric connections If the bench shall carry a complete HPLC system make sure that the bench is designed to bear the weight of all modules The module should be operated in a horizontal position Condensation CAUTION Condensation within the module Condensation will damage the system electronics gt Do not store ship or use your module under conditions where temperature fluctuations could cause condensation within the module gt If your module was shipped in cold weather leave it in its box and allow it to warm slowly to room temperature to avoid condensation 26 1290 Infinity TCC User Manual Site Requirements and Specifications 2 Physical Specifications Table3 Physical Specifications Type Specification Comments Weight 11 2 kg 22 Ibs Dimensions 140 x 345 x 435 mm 5 5 x 13 5 x 17 height x width x depth inches Line voltage 100 240 VAC 10 Wide ranging capability Line frequency 50 or 60 Hz 5 Power consumption 320 VA 150W 512 BTU Maximum Ambient operating 0 55 C 32 131 F temperature Ambient non operating 40 70 C 40 158 F temperature Humidity lt 95 r h at 40 C 104 F Non condensing Operating altitude Up to 2000 m 6562 ft Non operating altitude Up to 4600 m 15091 ft For storing the module Safety standards Installation category II
12. Agilent module to module 1 m Description Cross over network cable shielded 3 m for point to point connection Twisted pair network cable shielded 7 m for point to point connection 173 10 Identifying Cables RS 232 Cable Kit p n Description G1530 60600 RS 232 cable 2 m RS232 61601 RS 232 cable 2 5 m Instrument to PC 9 to 9 pin female This cable has special pin out and is not compatible with connecting printers and plotters It s also called Null Modem Cable with full handshaking where the wiring is made between pins 1 1 2 3 3 2 4 6 5 5 6 4 7 8 8 7 9 9 5181 1561 RS 232 cable 8 m 174 1290 Infinity TCC User Manual Identifying Cables 10 Agilent Module to Printer p n Description 5181 1529 Cable Printer Serial amp Parallel is a SUB D 9 pin female vs Centronics connector on the other end NOT FOR FW UPDATE For use with G1323 Control Module 1290 Infinity TCC User Manual 175 10 Identifying Cables Agilent Module to Printer 176 1290 Infinity TCC User Manual This chapter describes the TCC in more detail on hardware and electronics 1290 Infinity TCC User Manual 11 Hardware Information Firmware Description 178 Electrical Connections 181 Serial Number Information 182 Rear view of the module 183 Interfaces 184 Overview Interfaces 186 Setting the 8 bit Configuration Switch without On board LAN 190 Communication Settings for RS 232C 191 Special Settings 193 Early Maintenance Fee
13. Assure that the tie wrap does not block the front cover 118 1290 Infinity TCC User Manual Maintenance 8 Adding Heater Devices Adding Heater Devices The additional heater devices can be arranged in the G1316C in various locations depending on the application needs Some examples are shown below SS ii a coer Ete h Figure 28 Arrangements of Heater and Cooling Devices 1290 Infinity TCC User Manual 119 8 Maintenance Adding Heater Devices Figure 29 Typical positions of the low dispersion heat exchangers NOTE If the additional heater devices are used as shown above the column identification system cannot be used If the column identification system is required fix the heater devices in the upper or lower locations or fix them right left of the current location The heater devices are mounted on a carrier that can be fitted to the left and or right heat exchangers 120 1290 Infinity TCC User Manual Maintenance 8 Installing the Low Dispersion Heat Exchangers The positions of the low dispersion heat exchangers Heater long up 0 12 mm i d 1 6 uL internal volume G1316 80002 or Heater long down 0 12 mm i d 1 6 uL internal volume G1316 80003 depend very much on the desired application and columns you want to use with your system For two long columns gt 100 mm the carrier Carrier for heat exchanger G1316 89200 and the heat exchangers must be installed in a m
14. Introduction to Maintenance and Repair The module is designed for easy maintenance The most frequent maintenances such as maintaining valve heads if optional valve drive is installed or replacing low dispersion heat exchangers can be done from the front with module in place in the system stack There are no serviceable parts inside Do not open the module 112 1290 Infinity TCC User Manual Maintenance 8 Cautions and Warnings Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and reagents can hold health and safety risks gt When working with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice gt The volume of substances should be reduced to the minimum required for the analysis gt Do not operate the instrument in an explosive atmosphere CAUTION Hot heat exchangers A The column compartment has two heat exchanger assemblies that might be hot gt Allow them to cool down before starting repairs Electrical shock Repair work at the module can lead to personal injuries e g shock hazard when the cover is opened gt Do not remove the cover of the module gt Only certified persons are authorized to carry out repairs inside the module 1290 Infinity TC
15. carrier Carrier for heat exchanger G1316 89200 using 3 screws Screw 2 5 mm hex 0515 1052 included to part number for carrier as shown in figure below Build in heat exchanger Carrier for low dispersion heat exchanger Low dispersion heat exchanger Fitting holder assembly Figure 30 Installing the low dispersion heat exchangers 122 1290 Infinity TCC User Manual Maintenance 8 Choose Compatible Fittings For the heater device inlet capillary choose fittings which are compatible to your column The Very High Pressure Fitting is pressure tight up to 1200bar Do not overtighten the fitting at installation Finger tighten the nut into the port until snug Then use the wrench and apply about 1 4 of a revolution Torque 1 0 1 2 Nm Very High Pressure Fitting removable 5067 4738 for swagelock compatible columns Swagelock compatible columns 5065 4454 pack of 10 with ferrules om D Fitting screw long Figure 31 Fitting type depends on column type 1290 Infinity TCC User Manual 123 8 Maintenance Installing the capillaries Installing the capillaries Parts required Preparations 124 The 2pos 10port valve can be used here in the same way as a 2pos 6port valve just follow the re routing diagram below Map the ports from the 2pos 6port valve to the corresponding ports of the 2pos 10port valve according to the red arrows For example mount the capillary connected to port 6 2p
16. could result in damage to the product or loss of important data Do not proceed beyond a CAUTION notice until the indicated condi tions are fully understood and met A WARNING notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly performed or adhered to could result in personal injury or death Do not proceed beyond a WARNING notice until the indi cated conditions are fully under stood and met 1290 Infinity TCC User Manual In This Guide In This Guide This manual covers the Agilent 1290 Infinity Thermostatted Column Compartment TCC G1316C 1 Introduction to the Column Compartment This chapter gives an introduction to the TCC and an instrument overview 2 Site Requirements and Specifications This chapter provides information on environmental requirements physical and performance specifications 3 Installing the Module This chapter provides information on unpacking checking on completeness stack considerations and installation of the module 4 How to optimize the Column Compartment This chapter provides information on how to optimize the thermostatted column compartment 5 Troubleshooting and Diagnostics Overview about the troubleshooting and diagnostic features 6 Error Information This chapter describes the meaning of error messages and provides information on probable causes and suggested actions how to recover from e
17. mounted on carrier top for column 1 bottom for column 2 Figure 8 New additional heater devices The standard column compartment is equipped with a 3 ul and 6 ul heater or cooler Both can be set at the same or different temperature To reduce the delay volume a kit Standard Accessory Kit on page 155 has been set up for installing a small heater with 1 6 ul internal delay volume If the additional heater devices are used as shown in Figure 8 on page 34 the column identification system cannot be used If the column identification system is required fix the heater devices in the upper or lower locations or fix them right left of the current location 1290 Infinity TCC User Manual Site Requirements and Specifications 2 Solvent Information Observe the following recommendations on the use of solvents Follow recommendations for avoiding the growth of algae see pump manuals Small particles can permanently block capillaries and valves Therefore always filter solvents through 0 4 um filters Avoid or minimize the use of solvents that may corrode parts in the flow path Consider specifications for the pH range given for different materials like flow cells valve materials etc and recommendations in subsequent sections Solvent compatibility for stainless steel in standard LC systems Stainless steel is inert against many common solvents It is stable in the presence of acids and bases in the pH range specified for s
18. on the heat exchanger 7 Fasten all fittings on attached modules autosampler detector additional pumps Fit all unused valve ports with a plastic plug 8 Push the valves into the rear positions 1290 Infinity TCC User Manual 125 8 Maintenance Installing the capillaries 9 Place the capillaries that go to another module or waste into the capillary guides to prevent squeezing them when closing the front cover 10 Stow any excess lengths of the capillaries 11 Perform a final leak check 126 1290 Infinity TCC User Manual Maintenance 8 Configurations and Capillary Set up Configuration for dual column selection Figure 32 Installing the capillaries for a dual column selection set up column and heat exchanger of the second position are omitted 1 150 mm length column length up to 100 mm 280 mm length column length gt 100 mm From column Not pre swaged on column side 2 200 mm length to detector 3 150 mm length column length up to 100 mm 280 mm length column length gt 100 mm From column Not pre swaged on column side 4 Column with color code clip 5 Low dispersion heat exchanger or built in 3 pL 6 pL heat exchanger and 90 mm length capillary to column 6 90 mm length to heat exchanger 7 Built in 3 pL 6 uL heat exchanger and 90 mm length capillary to column 8 90 mm length to heat exchanger Pos 1 Connection between Ports 1 6 4 5 2 3 active Column 1 left Pos 2 Connection between Ports 1 2
19. 1 Introduction to the Column Compartment Typical Applications Dual column selection 2pos 6port valve G4231A B and 2pos 10port valve G4232A B C Advantages e Increase productivity e Higher instrument up time e Faster method scouting Quickly change between two different stationary phases to check your separation selectivity or use two identical stationary phases to have the second column immediately available after the first one loses efficiency for example with complex matrices Sample Enrichment and Sample Cleanup 2pos 6port valve G4231A B and 2pos 10port valve G4232A B C Advantages e Easy automation of sample preparation e Higher reproducibility Increased productivity and sensitivity Sample Enrichment Pump A autosampler Pump A autosampler Waste Position 1 18 Analytical i column Analytical detector couman Enrichment column Enrichment column Position 2 1290 Infinity TCC User Manual Introduction to the Column Compartment 1 Sample Cleanup Pump A autosampler Pump A autosampler Pre column Analytical Detector column Analytical Waste Detector column Position 1 Position 2 Sample cleanup is essential for samples with complex matrices such as biological fluids food extracts and waste water Before injection into a LC or LC MS system the sample matrix must be separated from the analytes of interest Otherwise contaminants can disrupt separation an
20. 89 Remote Timeout 90 Lost CAN Partner 90 Leak 91 Leak Sensor Open 91 Leak Sensor Short 92 TCC Error Messages 93 Compensation Sensor Open 93 Compensation Sensor Short 93 Left Fan Failed 94 Right Fan Failed 94 Open Cover 95 Cover Violation 95 Left Temperature Timeout 96 Right Temperature Timeout 96 Defective Temperature Sensor 97 Heater Profile 98 Column Temperature 98 Heatsink Temperature 99 Defective Heater Circuit 99 Valve failed to initialize 100 Unknown Unsupported Valve detected 100 Door sensor HW failure 101 Valve RFID access failure 101 This chapter describes the meaning of error messages and provides information on probable causes and suggested actions how to recover from error conditions egg Agilent Technologies 87 Error Information What Are Error Messages 88 Error messages are displayed in the user interface when an electronic mechanical or hydraulic flow path failure occurs which requires attention before the analysis can be continued for example repair or exchange of consumables is necessary In the event of such a failure the red status indicator at the front of the module is switched on and an entry is written into the module logbook If an error occurs outside a method run other modules will not be informed about this error If it occurs within a method run all connected modules will get a notification all LEDs get red and the run will be stopped Depending on the module type this stop is
21. Manual 5 Troubleshooting and Diagnostics Overview of the Module s Indicators and Test Functions 82 Status indicators 83 Power Supply Indicator 83 Module Status Indicator 84 Available Tests vs User Interfaces 85 Agilent Lab Advisor Software 86 Overview about the troubleshooting and diagnostic features Ee Agilent Technologies 81 5 Troubleshooting and Diagnostics Overview of the Module s Indicators and Test Functions Status Indicators The module is provided with two status indicators which indicate the operational state prerun run and error states of the module The status indicators provide a quick visual check of the operation of the module Error Messages In the event of an electronic mechanical or hydraulic failure the module generates an error message in the user interface For each message a short description of the failure a list of probable causes of the problem and a list of suggested actions to fix the problem are provided see chapter Error Information Thermostat Diagnostic Test The thermostat diagnostic test evaluates the heating and cooling efficiency of the two peltier elements Temperature Calibration and Verification The temperature calibration and verification procedure enables the instrument temperature to be measured against an external calibrated measuring device Normally temperature calibration is not required throughout the lifetime of the instrument However in order to co
22. PPPP is the product number for example 1315AB for the G1315A B DAD R the firmware revision for example A for G1315B or B for the G1315C DAD VVV is the revision number for example 102 is revision 1 02 XXX is the build number of the firmware For instructions on firmware updates refer to section Replacing Firmware in chapter Maintenance or use the documentation provided with the Firmware Update Tools Update of main system can be done in the resident system only Update of the resident system can be done in the main system only Main and resident firmware must be from the same set Main FW update Resident System Main System Resident FW Update Figure 43 Firmware Update Mechanism 1290 Infinity TCC User Manual 179 11 Hardware Information 180 Some modules are limited in downgrading due to their main board version or their initial firmware revision For example a G1315C DAD SL cannot be downgraded below firmware revision B 01 02 or to a A xx xx Some modules can be re branded e g G1314C to G1314B to allow operation in specific control software environments In this case the feature set of the target type are use and the feature set of the original are lost After re branding e g from G1314B to G1314C the original feature set is available again All these specific informations are described in the documentation provided with the firmware update tools The firmware update tools firmware and documentation a
23. Pump 2 Yes No Yes No Yes CAN DC OUT for CAN slaves Samplers G1329B ALS 2 Yes No Yes No Yes THERMOSTAT for G2260A Prep ALS G1330B K1330B G1364B FC PS 2 Yes No Yes No Yes THERMOSTAT for G1364C FC AS G1330B K1330B G1364D FC uS CAN DC OUT for CAN slaves G1367E HiP ALS K1367E HiP ALS Clinical Ed G1377A HiP micro ALS G2258A DL ALS G5664A Bio inert FC AS G5667A Bio inert Autosampler G4226A ALS 2 Yes No Yes No Yes Detectors G1314B VWD VL 2 Yes No Yes 1 Yes G1314C VWD VL G1314E F VWD 2 No Yes Yes 1 Yes K1314F Clinical Ed G4212A B DAD 2 No Yes Yes 1 Yes K4212B DAD Clinical Ed 184 1290 Infinity TCC User Manual Hardware Information 11 Table 26 Agilent 1200 Infinity Series Interfaces Module CAN LAN BCD LAN RS 232 Analog APG Special optional on board Remote G1315C DAD VL 2 No Yes Yes 2 Yes G1365C MWD G1315D DAD VL G1365D MWD VL G1321B FLD 2 Yes No Yes 2 Yes K1321B FLD Clinical Ed G1362A RID 2 Yes No Yes 1 Yes G4280A ELSD No No No Yes Yes Yes EXT Contact AUTOZERO Others G1170A Valve Drive 2 No No No No No 1 G1316A C TCC 2 No No Yes No Yes K1316C TCC Clinical Ed G1322A DEG No No No No No Yes AUX K1322A DEG Clinical Ed G1379B DEG No No No Yes No Yes G4225A DEG No No No Yes No Yes K4225A DEG Clinical Ed G4227A Flex Cube 2 No No No No No CAN DC OUT for CAN slaves 1 G4240A CHIP CUBE 2 Yes No Yes No Yes CAN DC OUT for CAN slaves THERMOSTAT for G1330A B NOT USED K1330B 1 R
24. a similar temperature level as the liquid running through the column This is done by thermal convection and radiation between the heat exchanger fins This design ensures that the column and the solvent flowing through it are almost at the same temperature Actual temperature control is accomplished at the heat exchanger The solvent cools down or heats up on its transfer from the heating block to the column inlet This depends on several factors flow rate setpoint temperature ambient temperature and column dimensions In a flow through temperature regulation system there are necessarily slightly different temperatures at different positions If for example the temperature set by the user is 40 C then the heat exchanger is regulated to a temperature of 40 8 C which is different by a certain offset here 0 8 C The solvent temperature at the column entry would be about 39 C The actual temperature displayed on the user interface is always the derived temperature taken at the heat exchanger corrected by the offset explained above Any type of heated column compartment brings one important consequence for column temperature equilibration Before an equilibrium is reached the whole mass of column column packing and solvent volume inside the column has to be brought to the selected temperature This depends on several factors flow rate setpoint temperature ambient temperature and column dimensions The higher the flow rate the
25. cause Suggested actions 1 Defective right heater assembly Please contact your Agilent service representative 2 Defective TCC board Please contact your Agilent service representative 96 1290 Infinity TCC User Manual Error Information 6 Defective Temperature Sensor Error ID 2821 One of the temperature sensors has failed The TCC board monitors the signal from the sensor continually If the signal is missing or out of range the error message is generated Defective Temperature Sensor 0 left column Defective Temperature Sensor 1 left heat sink Defective Temperature Sensor 2 right column Defective Temperature Sensor 3 right heat sink Defective Temperature Sensor 4 ambient correction sensor located on left flex board Probable cause Suggested actions 1 Flex board not connected only if all left or Please contact your Agilent service right sensor error messages appear representative simultaneously 2 Defective heater assembly Please contact your Agilent service representative 3 Defective TCC board Please contact your Agilent service representative 1290 Infinity TCC User Manual 97 98 Error Information Heater Profile Error ID Heater Profile 0 left heater Heater Profile 2 right heater The temperature warm up or cooling profile of the heater is incorrect When the temperature setpoint is changed the heater begins heating or cooling the column heat exchanger During this time the
26. column compartments as part of a method development solution make sure that the high pressure valve head is connected to the autosampler and the low pressure valve head is connected to the detector CAUTION Sample degradation and contamination of the instrument Metal parts in the flow path can interact with the bio molecules in the sample leading to sample degradation and contamination gt For bio inert applications always use dedicated bio inert parts which can be identified by the bio inert symbol or other markers described in this manual gt Do not mix bio inert and non inert modules or parts in a bio inert system 1290 Infinity TCC User Manual Installing the Module 3 The tag reader reads the valve head properties from the valve head RFID tag during initialization of the module Valve properties will not be updated if the valve head is replaced while the module is on Selection of valve port positions can fail if the instrument does not know the properties of the installed valve To have the valve correctly recognized by the Agilent Infinity valve drive you must have the valve drive powered off for at least 10 s 1290 Infinity TCC User Manual 53 3 Installing the Module The two different pins blue amp green on the valve shaft must fit into the corresponding grooves of the actuator axis The outside pin red must fit into the outside groove b It can be possible that you have to tur
27. dispersion It can be mounted in different locations Additionally available valve kits will have as many additional low dispersion heat exchangers as columns are supported Depending on the application these heater devices can be fixed at various places Information about the usage of these heater devices can be found in the Agilent 1290 Infinity LC System System Manual and Quick Reference G4220 90301 holes to attach carrier L shaped pre column heater Volume 1 6 ul mounted on carrier top for column 1 bottom for column 2 56 Figure 15 Installation points for heat exchanger If the additional heat exchangers are used as shown in this figure the column identification system cannot be used If the column identification system is required fix the heater devices in the upper or lower locations or right left of the current location 1290 Infinity TCC User Manual Installing the Module 3 nstallation of Heater Devices Installation of Heater Devices For the G1316C an additional heat exchanger can be installed on the Carrier for heat exchanger G1316 89200 using 3 screws Screw 2 5 mm hex 0515 1052 included to part number for carrier as shown in Figure 16 on page 57 Built in heat exchanger Figure 16 Installing the low dispersion heat exchangers 1290 Infinity TCC User Manual 57 3 Installing the Module Installation of Heater Devices Installation of the Bio Inert Solven
28. e Defective heater assembly Defective ambient temperature sensor Defective CCM board Installing the Temperature Sensor Installation of the temperature sensor is required for the temperature calibration and temperature verification procedures The figures below refer to a specific type of temperature sensor Heraeus Quat340 quartz surface temperature measurement sensor Other sensors may require a different fixing 1290 Infinity TCC User Manual 109 7 Test Functions and Calibrations Temperature Calibration 1 Remove the front cover 2 Install the temperature sensor at the measurement position on the left heat exchanger 3 Route the sensor wire through the slit in the leak tray 4 Re install the front cover 110 1290 Infinity TCC User Manual 1290 Infinity TCC User Manual 8 Maintenance Introduction to Maintenance and Repair 112 Cautions and Warnings 113 Overview of Maintenance 115 Cleaning the Column Compartment 116 Changing Column Identification Tags 117 Adding Heater Devices 119 Installing the capillaries 124 Correcting Leaks 131 Replacing Head Parts of Column Switching Valve 133 Replacing Valve Heads 136 Preparing the Column Compartment for Transportation 140 Replacing the Module Firmware 143 This chapter describes the maintenance tasks for the TCC If the instrument needs repair please contact your Agilent service representative ate Agilent Technologies 111 8 Maintenance
29. faster the column equilibrates due to thermostatted mobile phase 1290 Infinity TCC User Manual 11 1 Introduction to the Column Compartment Figure 1 on page 12 shows a setpoint temperature of 40 C Some time after entering the setpoint the heat exchanger has reached its temperature and the control activity starts The TEMPERATURE NOT READY signal will be cancelled 20 seconds after the sensed temperature was within a range of 0 5 C of the setpoint other values can be set via the user interface However this does not necessarily mean that the column has already reached the correct temperature The equilibration of the column may take longer Stability of the pressure signal is a good indication for equilibrium 45 Heat exchanger temperature 40 35 Column temperature Temperatur C 30 a Example for a flow rate of 5 ml min of water 0 1 2 3 4 5 6 7 8 9 0 N 2 8 4 6 6 T Time min Figure 1 Equilibration of Heat Exchanger and Column Temperature The temperature calibration and verification is described in the Service Manual 12 1290 Infinity TCC User Manual Introduction to the Column Compartment 1 Column Identification System The Agilent 1290 Infinity Thermostatted Column Compartment is equipped with a column identification system It allows to read and write column specific information to and from an optionally available column identification tag part of the HPLC starter kits available for 1220 1260 an
30. in order to store the values in the non volatile memory Table 30 Communication Settings for RS 232C Communication without on board LAN Mode Select 1 2 3 4 5 6 7 8 Rs 232c 0 1 Baudrate Data Bits Parity Use the following tables for selecting the setting which you want to use for RS 232C communication The number 0 means that the switch is down and 1 means that the switch is up 1290 Infinity TCC User Manual 191 11 Hardware Information 192 Table 31 Baudrate Settings without on board LAN Switches Baud Rate Switches Baud Rate 3 4 5 3 4 5 0 0 0 9600 1 0 0 9600 0 0 1 1200 1 0 1 14400 0 1 0 2400 1 1 0 19200 0 1 1 4800 1 1 1 38400 Table 32 Data Bit Settings without on board LAN Switch 6 Data Word Size 0 7 Bit Communication 1 8 Bit Communication Table 33 Parity Settings without on board LAN Switches Parity 7 e 0 0 No Parity 0 1 Odd Parity 1 1 Even Parity One start bit and one stop bit are always used not selectable Per default the module will turn into 19200 baud 8 data bit with no parity 1290 Infinity TCC User Manual Hardware Information 11 Special Settings The special settings are required for specific actions normally in a service case Boot Resident Firmware update procedures may require this mode in case of firmware loading errors main firmware part If you use the following switch settings and power the instr
31. latest information on products and services visit our worldwide web site on the Internet at http www agilent com 1290 Infinity TCC User Manual 205 Index Index 8 bit configuration switch without On Board LAN 190 A accessory kits 155 Agilent Lab Advisor software 86 Agilent Lab Advisor 86 Agilent on internet 205 ambient non operating temperature 27 ambient operating temperature 27 analog signal 187 analog cable 166 apg remote 188 B battery safety information 202 BCD cable 171 bench space 26 bio inert 52 114 materials 21 c cable analog 166 BCD 171 CAN 173 LAN 173 remote 168 206 RS 232 174 cables analog 164 BCD 164 CAN 165 LAN 165 overview 164 remote 164 RS 232 165 calibration temperature 107 82 CAN cable 173 cleaning 116 column clip 71 column identification 13 tag 70 column switching valve description 15 precolumn backflushing 17 two column selection 16 column temperature 98 column changing column and tags 117 Communication settings RS 232C 191 compensation sensor open 93 compensation sensor short 93 condensation 26 configuration one stack 42 two stack front 44 two stack rear 45 two stack 44 cool down time 28 cooling concept 11 cover violation 95 D dead volume 28 defect on arrival 40 defective heater circuit 99 defective temperature sensor 97 delay volume description 76 dimensions 27 door sensor hardware failure 101 E electrical connections descriptions
32. of 181 electronic waste 201 EMF early maintenance feedback 194 error messages column temperature 98 compensation sensor open 93 compensation sensor short 93 cover violation 95 defective heater circuit 99 defective temperature sensor 97 door sensor hardware failure 101 heater profile 98 heatsink temperature 99 leak sensor open 91 leak sensor short 92 leak 91 left fan failed 94 left temperature timeout 96 1290 Infinity TCC User Manual lost CAN partner 90 open cover 95 remote timeout 90 right fan failed 94 right temperature timeout 96 shutdown 89 timeout 89 unknown unsupported valve detected 100 valve failed to initialize 100 valve RFID access failure 101 external measuring device information 108 extra column volume description 77 F features GLP 28 28 safety and maintenace 28 firmware description 178 main system 1 8 resident system 178 update tool 179 updates 179 143 upgrade downgrade 143 frequency range 27 function test result 104 G GLP features 28 heater profile 98 heatexchanger pre column 34 heating concept 11 1290 Infinity TCC User Manual heatsink temperature 99 humidity 27 installation bench space 26 capillaries and waste tubings 61 65 column 65 65 60 61 temperature sensor 109 instrument layout 195 interfaces 184 internet 205 introduction concept of heating and cooling 11 system overview 11 L LAN cable 173 leak sensor open 91 leak sensor short 92 l
33. other markers described in this manual gt Do not mix bio inert and non inert modules or parts in a bio inert system 114 1290 Infinity TCC User Manual Overview of Maintenance Maintenance 8 The following pages describe maintenance procedures simple repairs that can be done without opening the main cover Table 20 Maintenance Procedures Procedure Typical Frequency Notes Cleaning the Column If required Compartment on page 116 Changing Column Identification Tags on page 117 Adding Heater Devices on page 119 Installing the capillaries on page 124 Correcting Leaks on page 131 Replacing Valve Heads on page 136 Preparing the Column Compartment for Transportation on page 140 Replacing the Module Firmware on page 143 When column performance or new application requires a change When new application requires a change When new application requires a change If a leak has occurred Check for leaks If the valve performance shows indication of leakage or wear If the TCC shall be transported If required 1290 Infinity TCC User Manual 115 8 Maintenance Cleaning the Column Compartment To keep the module case clean use a soft cloth slightly dampened with water or a solution of water and mild detergent WARNING Liquid dripping into the electronic compartment of your module can cause shock hazard and damage the module gt Do n
34. side Otherwise the integrator prints START not ready 1290 Infinity TCC User Manual Identifying Cables Agilent Module to 3396 Series III 3395B Integrators 10 p n 03396 61010 Pin 33XX Pin Agilent Signal Name Active module TTL 9 1 White Digital ground NC 2 Brown Prepare run Low 80 i 3 3 Gray Start Low z NC 4 Blue Shut down Low e an NC 5 Pink Not is connected NC 6 Yellow Power on High 14 7 Red Ready High 4 8 Green Stop Low NC 9 Black Start request Low 13 15 Not connected 1290 Infinity TCC User Manual 169 10 170 Identifying Cables Agilent Module to Agilent 35900 A D Converters p n 5061 3378 Pin 35900 Pin Agilent Signal Name Active A D module TTL 1 White 1 White Digital ground 2 Brown 2 Brown Prepare run Low 3 Gray 3 Gray Start Low 4 Blue 4 Blue Shut down Low 5 Pink 5 Pink Not connected 6 Yellow 6 Yellow Power on High 7 Red 7 Red Ready High 8 Green 8 Green Stop Low 9 Black 9 Black Start request Low Agilent Module to General Purpose p n 01046 60201 Wire Color Pin Agilent Signal Name Active module TTL E White 1 Digital ground n lol i Brown 2 Prepare run Low 00 pee DE KEY Gray 3 Start Low oo o0 S EE Blue 4 Shut down Low ug Pink 5 Not og connected 15 o Yellow 6 Power on High Red 7 Ready High Green 8 Stop Low Black 9 Start
35. the cycle time Typically the column needs flushing with three to five times the column volume to stabilize it ready for the next injection and this can be 50 or more of the separation time in some applications It is an essential process but can be taken out of the cycle time by using automated alternating column regeneration For this the following apparatuses are required available at the local Agilent sales representative e A special valve drive available as accessory kit G1353B For installation please contact your Agilent service representative e A second analytical column identical to the first A second pump As one column is being used in the separation run the other column is being flushed with the starting composition of the mobile phase gradient and to start the next injection the newly re equilibrated column is switched into the analytical flow path The two columns then alternate in this way for the whole sequence of injections The second pump is only required to flush an isocratic mixture through the column and so can be a simpler pump than the 1290 Infinity pumps For instance a 1200 Series isocratic pump would be sufficient to perform this task The setup is illustrated in Table on page 80 Eluent pump Eluent pump ett Autosampler Autosampler Regeneration Regeneration pump pump Position 2 Position 1 80 1290 Infinity TCC User Manual 1290 Infinity TCC User
36. therefore the material of choice for biochemical instrumentation It is stable in the specified pH range and inert to many common solvents There is still a number of known incompatibilities with chemicals such as chloroform methylene chloride THF DMSO strong acids nitric acid gt 10 sulphuric acid gt 10 sulfonic acids trichloroacetic acid halogenes or aequous halogene solutions phenol and derivatives cresols salicylic acid etc 1290 Infinity TCC User Manual Site Requirements and Specifications 2 When used above room temperature PEEK is sensitive to bases and various organic solvents which can cause it to swell As normal PEEK capillaries are very sensitive to high pressure especially under such conditions Agilent uses stainless steel cladded PEEK capillaries to keep the flow path free of steel and to ensure pressure stability to at least 600 bar If in doubt consult the available literature about the chemical compatibility of PEEK Titanium Titanium is highly resistant to oxidizing acids for example nitric perchloric and hypochlorous acid over a wide range of concentrations and temperatures This is due to a thin oxide layer on the surface which is stabilized by oxidizing compounds Reducing acids for example hydrochloric sulfuric and phosphoric acid can cause slight corrosion which increases with acid concentration and temperature For example the corrosion rate with 3 HCl about pH 0 1 at room temper
37. 1 4 5 16 inch for capillary connections Preparations Install the column compartment WARNING Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and reagents can hold health and safety risks gt When working with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice gt The volume of substances should be reduced to the minimum required for the analysis gt Do not operate the instrument in an explosive atmosphere 1290 Infinity TCC User Manual 63 3 Installing the Module Flow Connections of the Column Compartment 1 Press release buttons and remove front cover to gain access to heater area 2 The column compartment is equipped with a column identification system that can read column tags Tag readers Column tag Column clip t NOTE For more information on column identification see Column ldentification System on page 13 NOTE The internal volumes of the heat exchanger assemblies comprise a volume of 3 ul left and 6 pl right The internal capillary diameter is 0 17 mm 64 1290 Infinity TCC User Manual Installing the Module 3 Flow Connections of the Column Compartment 3 Place the column on the left heat exchanger assembly and c
38. 1B Agilent 1290 Series 2 Position 6 Port valve head 1200bar Type Specification Liquid contacts Vespel Stainless Steel Port size Accepts 10 32 male threaded fittings Flow passage diameters Stator 0 38 mm 0 015 in Rotor Seal 0 30 mm 0 012 in Port to Port Volume 0 51 uL Maximum pressure 1200 bar Table 9 G4232A Agilent 1290 Series 2 Position 10 Port micro valve head 600bar Type Specification Liquid contacts PEEK Stainless Steel Port size Accepts M4 male threaded fittings Flow passage diameters 0 20 mm 0 0108 in Port to Port Volume 89 nL Maximum pressure 600 bar Table 10 G4232B Agilent 1290 Series 2 Position 10 Port valve head 1200bar Type Specification Liquid contacts Vespel Stainless Steel Port size Accepts 10 32 male threaded fittings Flow passage diameters Stator 0 15 mm 0 006 in Rotor Seal 0 20 mm 0 008 in Port to Port Volume 0 22 uL Maximum pressure 1200 bar 1290 Infinity TCC User Manual Site Requirements and Specifications 2 Table 11 G4232C Agilent 1290 Series 2 Position 10 Port valve head 600bar Type Specification Liquid contacts PEEK Stainless Steel Port size Accepts 10 32 male threaded fittings Flow passage diameters Stator 0 15 mm 0 006 in Rotor Seal 0 20 mm 0 008 in Port to Port Volume 0 16 pL Maximum pressure 600 bar Table 12 G4234A 6 Column Selection Valve 600bar Type Specification Liquid contacts PEEK Stainle
39. 2013 please follow procedure as described in the Appendix of the Agilent 1260 Infinity Bio inert Quaternary LC System Manual 1 Slide the fitting on the capillary Let the capillary jut out 5 mm Capillary Fitting 1290 Infinity TCC User Manual 67 3 Installing the Module Installing Capillaries 2 Insert the fitting to the receiving port and push the capillary to the bottom of the port 3 Finger tighten the nut into the port until snug 68 1290 Infinity TCC User Manual Installing the Module 3 Installing Capillaries 4 Use Fitting mounting tool 5043 0915 or a 5 mm hex wrench for fixing the fitting maximum torque 0 8 Nm 1290 Infinity TCC User Manual 69 3 Installing the Module Placing Columns Column ldentification Tag When correctly placed on the heat exchanger the distance between the column identification tag and the tag reader is 1 2 mm This is the optimum distance for proper function The identification tag can be easily removed from the column For columns with small diameter a cable tie wrap should be used to fix the column identification tag to the column Assure that the tie wrap does not block the front cover The tag needs to be placed differently depending on whether the column is installed at the left or right heat exchanger see Figure 19 on page 70 and Figure 20 on page 71 The Agilent logo should always be at front Column identification tag Figure 1
40. 3 4 5 6 active Column 2 right Example shows setup with flow directed 1290 typical from bottom to top Flow direction from top to bottom needs switch of connected capillaries at ports 5 and 2 Also column inlet connections needed to be switched with outlet connections Port 4 to 3 and 6 to 1 1290 Infinity TCC User Manual 127 Maintenance iInst lling the capillar Configuration for sample enrichment 128 Figure 33 Installing the capillaries for a sample enrichment set up 10 11 Analytical column with color code clip 280 mm length column length gt 100 mm from analytical column to detector Not pre swaged on column side 340 mm length from autosampler and loading pump To waste 150 mm length column length up to 100 mm 280 mm length column length gt 100 mm From column Not pre swaged on column side Enrichment column with color code clip Low dispersion heat exchanger or build in 3 pL 6 pL heat exchanger and 90 mm length capillary to column 90 mm length to heat exchanger 700 mm length 0 17 mm ID from analytical pump 90 mm length to heat exchanger Low dispersion heat exchanger or build in 3 pL 6 pL heat exchanger and 90 mm length capillary to column Pos 1 Connection between ports 1 6 4 5 2 3 active column 1 left enrichment column Pos 2 Connection between ports 1 2 3 4 5 6 active column 2 right analytical colum 1290 Infinity TCC User Manual Maintenance 8 Installing the cap
41. 4 6 integrators Agilent 35900A A D converter Analog cable BNC to general purpose spade lugs Description Agilent module to 3396A Series integrators 3396 Series II 3395A integrator see details in section Remote Cables on page 168 Agilent module to 3396 Series III 3395B integrators Remote Cable Agilent module to general purpose Description Agilent module to 3396 integrators Agilent module to general purpose 1290 Infinity TCC User Manual CAN cables p n 5181 1516 5181 1519 LAN cables p n 5023 0203 5023 0202 RS 232 cables p n G1530 60600 RS232 61601 5181 1561 1290 Infinity TCC User Manual Identifying Cables 10 Description CAN cable Agilent module to module 0 5 m CAN cable Agilent module to module 1 m Description Cross over network cable shielded 3 m for point to point connection Twisted pair network cable shielded 7 m for point to point connection Description RS 232 cable 2 m RS 232 cable 2 5 m Instrument to PC 9 to 9 pin female This cable has special pin out and is not compatible with connecting printers and plotters It s also called Null Modem Cable with full handshaking where the wiring is made between pins 1 1 2 3 3 2 4 6 5 5 6 4 7 8 8 7 9 9 RS 232 cable 8 m 165 10 Identifying Cables Analog cables One end of these cables provides a BNC connector to be connected to Agilent modules The other end depends on the instrument to whic
42. 8 Replacing Valve Heads 7 Install all required capillary connections to the valve 8 Push the valve head until it snaps in and stays in the rear head position 9 Switch on the module 1290 Infinity TCC User Manual 139 8 Maintenance Preparing the Column Compartment for Transportation When If a Thermostatted Column Compartment including the Valve Drive Option shall be transported Tools required Description Screwdriver Pozidriv 1 PT3 Parts required p n Description 1 G1316 67001 Transportation Lock Kit The module has been shipped with transportation locks which must be used for transportation protection Transportation Lock Kit G1316 67001 can be re ordered CAUTION Damage to Internal Parts Mechanical shocks for example when being transported by car or shipped by post gt Install a lock Transportation Lock Kit 140 1290 Infinity TCC User Manual Maintenance 8 Preparing the Column Compartment for Transportation 1 Remove the valve head as described in Replacing Valve 2 Replace the valve head by the transportation valve head Heads on page 136 Bring the transportation valve head to the outer position 1290 Infinity TCC User Manual 141 Maintenance Preparing the Column Compartment for Transportation 3 Use Screw M3x8 pack of 4 0515 0897 for fixing the Lock plate G1316 03701 to the heat exchangers in the outer positions of the plate 4 U
43. 9 Column ldentification Tag for Left Heat Exchanger 70 1290 Infinity TCC User Manual Installing the Module 3 Column identification tag Figure 20 Column ldentification Tag for Right Heat Exchanger Column Clip For better positioning of the column on the heat exchanger a column clip is available see Accessory Kits on page 155 Figure 21 Column clip 1290 Infinity TCC User Manual 71 3 Installing the Module Placing Columns 72 1290 Infinity TCC User Manual 1290 Infinity TCC User Manual 4 How to optimize the Column Compartment Optimizing the Column Compartment 74 Using Additional Heater Devices 75 Delay Volume and Extra Column Volume 76 Delay Volume 76 Extra Columwn Volume 77 How to Configure the Optimum Delay Volume 78 How to Achieve Higher Injection Volumes 79 How to Achieve High Throughput 80 This chapter provides information on how to optimize the thermostatted column compartment Apg Agilent Technologies 73 4 How to optimize the Column Compartment Optimizing the Column Compartment 74 For best performance results of the column compartment Use short connection capillaries and place them close to the heat exchanger This will reduce heat dissipation and external band broadening Use the left heat exchanger for small volume columns for example 2 3 mm i d columns at flow rates of less than 200 uL min Use the low dispersion heat exchanger to minimize peak dispersion and dela
44. 9 Parts and Materials for Maintenance Capillary kit PN 5067 4730 The capillary kit PN 5067 4730 contains the following parts p n Description 5067 4723 Capillary ST 0 17 mm x 340 mm S SX Autosampler to Valve 5067 4648 Capillary ST 0 17 mm x 700 mm S SX Pump to valve ACR only 5067 4724 4x Capillary ST 0 17 mm x 90 mm S SX Valve to heat exchanger and heat exchanger to column 5067 4720 2x Capillary ST 0 17 mm x 150 mm SL SX short column to valve 5067 4722 2x Capillary ST 0 17 mm x 280 mm SL SX long column to valve 5067 4719 Capillary ST 0 17 mm x 120 mm SX SX valve to valve bypass 5067 4721 Capillary ST 0 17 mm x 200 mm S SX valve to detector 0890 1713 Tube PTFE 2 m valve to waste 5042 9918 Column clip set eight colors 160 1290 Infinity TCC User Manual Parts and Materials for Maintenance 9 Capillary kit PN 5067 4729 The capillary kit PN 5067 4729 contains the following parts p n 5067 4744 5067 4745 5067 4735 8x 5067 4737 5067 4746 8710 1534 5022 6503 64240 43200 61316 80002 2x 61316 80003 2x 61316 89200 2x 61316 68706 5042 9918 1290 Infinity TCC User Manual Description Capillary ST 0 12 mm x 340 mm SL M Autosampler to Valve Capillary ST 0 11 mm x 500 mm S M Sampler dual stack to Valve Capillary ST 0 11 mm x 130 mm LS M Valve to heat exchanger and column to valve Capillary ST 0 17 mm x 150 mm M M Valve to Valve bypass Capillary ST 0 12 mm x 250 mm
45. C User Manual 113 8 Maintenance The module is partially energized when switched off as long as the power cord is plugged in Repair work at the module can lead to personal injuries e g electrical shock when the cover is opened and the module is connected to power gt Always unplug the power cable before opening the cover gt Do not connect the power cable to the instrument while the covers are removed Personal injury or damage to the product Agilent is not responsible for any damages caused in whole or in part by improper use of the products unauthorized alterations adjustments or modifications to the products failure to comply with procedures in Agilent product user guides or use of the products in violation of applicable laws rules or regulations gt Use your Agilent products only in the manner described in the Agilent product user guides CAUTION Safety standards for external equipment gt If you connect external equipment to the instrument make sure that you only use accessory units tested and approved according to the safety standards appropriate for the type of external equipment CAUTION Sample degradation and contamination of the instrument Metal parts in the flow path can interact with the bio molecules in the sample leading to sample degradation and contamination gt For bio inert applications always use dedicated bio inert parts which can be identified by the bio inert symbol or
46. CC User Manual ee 7 r Test Functions and Calibrations Thermostat Function Test 104 Pressure Test 106 Temperature Calibration 107 Temperature Calibration Procedure 108 Calibration Problems 109 Installing the Temperature Sensor 109 This chapter describes the TCC s built in test functions Ee Agilent Technologies 103 7 Test Functions and Calibrations Thermostat Function Test Thermostat Function Test Description The Thermostat Function Test is used to evaluate the cooling and heating performance of the two peltier elements When the test is started both heat exchangers are cooled initially to 25 C This temperature is held for 12 seconds and then the setpoint is changed to 20 C The time required to reach 20 C is a measure of the cooling efficiency of the peltier elements At 3 5 minutes the setpoint is changed to 30 C and both elements begin heating The time required to reach 30 C is a measure of heating efficiency Thermostat Function Test Result A typical Thermostat Function Test profile is shown in Figure 23 on page 104 Temperature C 304 Left Peltier element Right Peltier element 104 T T T T i T 1 2 i g 4 5 Time minutes Figure 23 Typical Thermostat Function Test Profile 1290 Infinity TCC User Manual Test Functions and Calibrations 7 Evaluating the Thermostat Function Test During the cooling phase the Peltier elements should cool at a rate of
47. Description Any Agilent Quick Change Valve Head For details see Valve Options Overview on page 153 WARNING Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and reagents can hold health and safety risks gt Be sure that no solvent can drop out of the solvent connections when removing them from your valve head gt When working with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice CAUTION Valve Damage Using a low pressure valve on the high pressure side can damage the valve gt When using multiple column compartments as part of a method development solution make sure that the high pressure valve head is connected to the autosampler and the low pressure valve head is connected to the detector 136 1290 Infinity TCC User Manual Maintenance 8 For details please refer to the Agilent 1200 Infinity Series Method Development System System Manual G4230 90002 CAUTION Column Damage or Bias Measurement Results Switching the valve to a wrong position can damage the column or bias measurement results gt Fit the lobe to the groove to make sure the valve is switched to the correct position The valve actuator contains sensitive optical parts which need to be protected fro
48. F Solvent information for parts of the 1260 Infinity Bio inert LC system For the Agilent 1260 Infinity Bio inert LC system Agilent Technologies uses highest quality materials see Bio inert Materials on page 21 in the flow path also referred to as wetted parts which are widely accepted by life scientists as they are known for optimum inertness to biological samples and ensure best compatibility to common samples and solvents over a wide pH range Explicitly the complete flow path is free from stainless steel and free from other alloys containing metals such as iron nickel cobalt chromium molybdenum or copper which can interfere with biological samples The flow downstream of the sample introduction contains no metals whatsoever However there are no materials that combine suitability for versatile HPLC instrumentation valves capillaries springs pump heads flow cells etc with complete compatibility with all possible chemicals and application conditions This section recommends the preferred solvents Chemicals that are known to cause issues should be avoided or exposure should be minimized for example for short term cleaning procedures After potentially aggressive chemicals have been used the system should be flushed with compatible standard HPLC solvents PEEK PEEK Polyether Ether Ketones combines excellent properties with regard to biocompatibility chemical resistance mechanical and thermal stability and is
49. Pollution degree2 For indoor use only IEC CSA UL 1290 Infinity TCC User Manual 27 2 Site Requirements and Specifications Performance Specifications 28 Table 4 Performance Specifications Thermostatted Column Compartment Type Specification Comments Temperature range Temperature stability Temperature accuracy Column capacity Warm up cool down time Dead volume Communications Safety and maintenance GLP features Housing 10 degrees below ambient to 100 C up to 80 C flow rates up to 5 ml min up to 100 C flow rates up to 2 5 ml min 0 05 C 0 8 C 0 5 C Three 30 cm With calibration 5 min from ambient to 40 C 10 min from 40 20 C 1 6 pl low dispersion heat exchanger 3 pl left heat exchanger 6 ul right heat exchanger Controller area network CAN RS 232C APG Remote ready start stop and shut down signals LAN via other modules i d 0 12 mm capillary kit available i d 0 17 mm standard Extensive support for troubleshooting and maintenance is provided by the Instant Pilot Agilent Lab Advisor and the Chromatography Data System Safety related features are leak detection safe leak handling leak output signal for shutdown of pumping system and low voltages in major maintenance areas Column identification module for GLP documentation of column type All materials recyclable All specifications are valid for distilled water at amb
50. Restart the analysis where required An external instrument has generated a shutdown signal on the remote line The module continually monitors the remote input connectors for status signals A LOW signal input on pin 4 of the remote connector generates the error message Probable cause 1 Leak detected in another module with a CAN connection to the system 2 Leak detected in an external instrument with a remote connection to the system 3 Shut down in an external instrument with a remote connection to the system 4 The degasser failed to generate sufficient vacuum for solvent degassing 1290 Infinity TCC User Manual Suggested actions Fix the leak in the external instrument before restarting the module Fix the leak in the external instrument before restarting the module Check external instruments for a shut down condition Check the vacuum degasser for an error condition Refer to the Service Manual for the degasser or the 1260 pump that has the degasser built in 89 90 Error Information Remote Timeout Error ID 0070 A not ready condition is still present on the remote input When an analysis is started the system expects all not ready conditions for example a not ready condition during detector balance to switch to run conditions within one minute of starting the analysis If a not ready condition is still present on the remote line after one minute the error message is generated Probable ca
51. SV M Valve to Detector Wrench 4 mm both ends open end Flexible PEEK tubing 450 mm 0 4 mm i d Valve to Waste PEEK fitting special for Chip LC Heat Exchanger Long Up 1 6 pL Heat Exchanger Long Down 1 6 pL Carrier for heat exchanger TCC SL Plus Fitting holder assembly Column clip set eight colors 161 162 9 Parts and Materials for Maintenance Accessories The Transportation Lock Kit G1316 67001 has been delivered with the instrument You will need this kit to prepare the TCC for transportation Transportation Lock Kit G1316 67001 p n Description G1316 03701 Lock plate 2680 0128 Screw M4 0515 0897 Screw M3x8 pack of 4 Spring Washer G1316 40002 Transportation valve head 1290 Infinity TCC User Manual This chapter summarizes information on all cables 1290 Infinity TCC User Manual 10 Identifying Cables Cable Overview 164 Analog cables 166 Remote Cables 168 BCD Cables 171 CAN Cable 173 RS 232 Cable Kit 174 Agilent Module to Printer 175 ee Agilent Technologies 163 10 Identifying Cables Cable Overview 164 Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Analog cables p n 35900 60750 35900 60750 01046 60105 Remote cables p n 03394 60600 03396 61010 5061 3378 01046 60201 BCD cables p n 03396 60560 G1351 81600 Description Agilent module to 339
52. ad to the valve drive of a TCC For the installation of a valve head to a G1170A Infinity Valve Drive you can ignore the steps that describe the TCC features of the transportation lock and spring loaded valve drive 3 1 When unscrewing the transportation lock TCC only push it back until the last screw is removed the valve rail is spring loaded 2 To remove the valve dummy loosen the nut manually K OT 1290 Infinity TCC User Manual 51 3 Installing the Module Installing the Valve Head and Connecting Capillaries CAUTION For bio inert modules use bio inert parts only Linert The valve actuator contains sensitive optical parts which need to be protected from dust and other pollutions Pollution of these parts can impair the accurate selection of valve ports and therefore bias measurement results gt Always install a valve head for operation and storage For protecting the actuator a dummy valve head part of Transportation Lock Kit G1316 67001 can be used instead of a functional valve Do not touch parts inside the actuator CAUTION Column Damage or Bias Measurement Results Switching the valve to a wrong position can damage the column or bias measurement results gt Fit the lobe to the groove to make sure the valve is switched to the correct position CAUTION Valve Damage Using a low pressure valve on the high pressure side can damage the valve gt When using multiple
53. anual 41 3 Installing the Module Optimizing the Stack Configuration One Stack Configuration Ensure optimum performance by installing the modules of the Agilent 1290 Infinity Binary LC System in the following configuration See Figure 9 on page 42 and Figure 10 on page 43 This configuration optimizes the flow path for minimum delay volume and minimizes the bench space required The Agilent 1290 Infinity Binary Pump should always be installed at the bottom of the stack Instant Pilot Solvent cabinet Detector Column compartment Autosampler Pump E Sr Figure 9 Recommended stack configuration for 1290 Infinity with binary pump front view 42 1290 Infinity TCC User Manual Installing the Module Optimizing the Stack Configuration LAN to LC ChemStation Wu Mi CAN Bus cable to Instant Pilot Analog detector signal optional AC Power CAN Bus cable Figure 10 Recommended stack configuration 1290 Infinity with binary pump rear view 1290 Infinity TCC User Manual 3 43 3 Installing the Module Optimizing the Stack Configuration Two Stack Configuration In case the autosampler thermostat is added to the system a two stack configuration is recommended which places both heavy modules 1290 Infinity pump and thermostat at the bottom of each stack and avoids high stacks Some users prefer the lower height of this arran
54. ature is about 13 um year At room temperature titanium is resistant to concentrations of about 5 sulfuric acid about pH 0 3 The addition of nitric acid to hydrochloric or sulfuric acids significantly reduces corrosion rates Titanium is subject to corrosion in anhydrous methanol which can be avoided by adding a small amount of water about 3 Slight corrosion is possible with ammonia gt 10 Fused silica Fused silica is inert against all common solvents and acids except hydrofluoric acid It is corroded by strong bases and should not be used above pH 12 at room temperature The corrosion of flow cell windows can negatively affect measurement results For a pH greater than 12 the use of flow cells with sapphire windows is recommended Gold Gold is inert to all common HPLC solvents acids and bases within the specified pH range It can be corroded by complexing cyanides and concentrated acids like aqua regia a mixture of concentrated hydrochloric and nitric acid Zirconium Oxide Zirconium Oxide ZrO is inert to almost all common acids bases and solvents There are no documented incompatibilities for HPLC applications 1290 Infinity TCC User Manual 37 2 38 Site Requirements and Specifications Platinum Iridium Platinum Iridium is inert to almost all common acids bases and solvents There are no documented incompatibilities for HPLC applications PTFE PTFE polytetrafluorethen is inert to almost all comm
55. column to valve Capillary ST 0 12 mm x 280 mm SL SX Long Column to Valve Capillary ST 0 12 mm x 120 mm SX SX Valve to Valve bypass Capillary ST 0 12 mm x 200 mm S SX Valve to Detector Tube PTFE 2 m Valve to Waste Heat Exchanger Long Up 1 6 pL Heat Exchanger Long Down 1 6 pL Carrier for heat exchanger TCC SL Plus Fitting holder assembly Column clip set eight colors Plastic fitting 1290 Infinity TCC User Manual Parts and Materials for Maintenance 9 Capillary kit PN 5067 4682 The capillary kit PN 5067 4682 contains the following parts p n 5067 4684 5067 4648 5067 4685 2x 5067 4686 2x 5067 4687 5067 4688 5067 4689 0890 1713 61316 80002 61316 80003 61316 89200 2x 61316 68706 2x 0100 1259 4x 5042 9918 1290 Infinity TCC User Manual Description Capillary ST 0 12 mm x 340 mm S SX Autosampler to Valve Capillary ST 0 17 mm x 700 mm S SX Pump to valve ACR only Capillary ST 0 12 mm x 90 mm S SX Valve to heat exchanger Capillary ST 0 12 mm x 150 mm SX SX short column to valve Capillary ST 0 12 mm x 280 mm SX SX long column to valve Capillary ST 0 12 mm x 120 mm SX SX valve to valve bypass Capillary ST 0 12 mm x 200 mm S SX valve to detector Tube PTFE 2 m valve to waste Heat Exchanger Long Up 1 6 pL Heat Exchanger Long Down 1 6 pL Carrier for heat exchanger TCC SL Plus Fitting holder assembly Plastic fitting Column clip set eight colors 159
56. d 1290 Infinity pumps or separately as PN 5062 8588 Tag readers w olumn clip Figure 2 Column ldentification System Table 1 on page 14 shows the information that can be stored The information fields can be edited via the user interface 1290 Infinity TCC User Manual 13 1 Introduction to the Column Compartment Table 1 Column ldentification Module Information Item Example Comment Product number Serial number Batch number 799160D 552 950522 Date of manufacturing 1675 Geometry 100 mm x 2 1 mm Stationary phase ODS Hypersil Particle size 10 um Number of injections 1267 See below Maximum pressure allowed 400 bar Maximum temperature recommended 70 C Maximum pH recommended 12 14 Column void volume The number of injections will be updated each run to create a column lifecycle history The user interface allows to edit all information If a 2 Position 6 Port valve and 2 Position 10 Port valve see Column Switching Valve Optional on page 15 is installed in the module the update of the number of injections depends on the position of the column switching valve If the valve connects the left column to the flow path the left but not the right column tag is updated and vice versa If no column switching valve is installed both sides are updated at the same time If an 8 Position 9 Port valve is installed no tag is updated for avoiding ambiguity as more than 2 columns might be instal
57. d detection or even damage the analytical column Enrichment methods Enrichment methods are the technique of choice to obtain highest sensitivity and to remove the sample matrix in such applications as proteomics drug metabolism and environmental trace analysis The analytes are retained and concentrated onto the pre column while the sample matrix is passed to waste After the valve switch a second pump backflushes the analytes out of the pre column onto the separation column This allows injection of large volumes onto the pre column significantly expanding sensitivity in the range of ten to several thousands Stripping methods Stripping methods handle analytes and matrices in the opposite way to enrichment methods Matrix components are retained on the pre column while the analytes pass through to the separation column After the valve switches an additional pump backflushes the matrix components out of the pre column to waste while the analytes are separated on the main column Backflushing prepares the pre column for the next injection 1290 Infinity TCC User Manual 19 1 20 Eluent pump Autosampler Regeneration pump Position 1 Introduction to the Column Compartment Alternating Column Regeneration Only 2pos 10port valve G4232A B C Advantages High sample throughput Increased productivity e High efficiency Alternating column regeneration is a convenient way to increase the sample throughput T
58. dback 194 Instrument Layout 195 ee Agilent Technologies 177 11 Hardware Information Firmware Description The firmware of the instrument consists of two independent sections e anon instrument specific section called resident system e an instrument specific section called main system Resident System This resident section of the firmware is identical for all Agilent 1100 1200 1220 1260 1290 series modules Its properties are the complete communication capabilities CAN LAN and RS 232C memory management e ability to update the firmware of the main system Main System Its properties are the complete communication capabilities CAN LAN and RS 232C memory management e ability to update the firmware of the resident system In addition the main system comprises the instrument functions that are divided into common functions like e run synchronization through APG remote error handling diagnostic functions e or module specific functions like internal events such as lamp control filter movements raw data collection and conversion to absorbance 178 1290 Infinity TCC User Manual Hardware Information 11 Firmware Updates Firmware updates can be done using your user interface PC and Firmware Update Tool with local files on the hard disk e Instant Pilot G4208A with files from a USB Flash Disk Agilent Lab Advisor software B 01 03 and above The file naming conventions are PPPP_RVVV_XXX dlb where
59. detected an internal problem which affects correct operation of the module Usually an error condition requires attention e g leak defective internal components An error condition always interrupts the analysis If the error occurs during analysis it is propagated within the LC system i e ared LED may indicate a problem of a different module Use the status display of your user interface for finding the root cause module of the error A blinking indicator indicates that the module is in resident mode e g during update of main firmware A fast blinking indicator indicates that the module is in a low level error mode In such a case try to re boot the module or try a cold start see Special Settings on page 193 Then try a firmware update see Replacing the Module Firmware on page 143 If this does not help a main board replacement is required 1290 Infinity TCC User Manual Troubleshooting and Diagnostics 5 Available Tests vs User Interfaces Depending on the user interface the available tests and the screens reports may vary see chapter Test Functions and Calibrations e Preferred tool should be the Agilent Lab Advisor software see Agilent Lab Advisor Software on page 86 The Agilent ChemStation B 04 02 and above may not include any maintenance test functions Screenshots used within these procedures are based on the Agilent Lab Advisor software 1290 Infinity TCC User Manual 85 5 Tr
60. e Module 4 Ensure the power switch at the front of the column compartment is OFF Status indicator green yellow red Line power switch with green light Figure 13 Front View of the Thermostatted Column Compartment 5 At the rear of the module move the security lever to its maximum right position Modules with sheet metal kits manufactured after 2011 are delivered without security lever 6 Connect the power cable to the power connector at the rear of the module The security lever will prevent that the cover is opened while the power cord is connected to the module 48 1290 Infinity TCC User Manual Installing the Module 3 Installing the Column Compartment 7 Connect the required interface cables to the rear of the module RS 232 C APG Remote Configuration switch Security lever i gt gt See amp Figure 14 Rear view of the module NOTE Modules with sheet metal kits manufactured after 2011 are delivered without security lever 8 If Agilent ChemStation is the controller connect the LAN connection to the LAN interface in the detector 9 Connect the APG remote cable optional for non Agilent instruments 10 Turn on the power by pushing the button at the lower left hand side of the module The power button stays pressed in and the status LED should be green NOTE When the line power button stands out and the green light is off the module is tu
61. e TCC board detects when the top foam is in place If the foam is removed the fans and peltier elements are switched OFF and the error message is generated Probable cause Suggested actions 1 The top foam was removed during Please contact your Agilent service operation representative 2 Foam not activating the sensor Please contact your Agilent service representative Cover Violation Error ID 2833 The column compartment was switched on with the top cover and foam open The sensor on the CCM board detects if the top foam is in place If the column compartment is switched on with the foam removed the processor switches OFF the peltier elements after a short delay and the error message is generated Probable cause Suggested actions 1 The column compartment was switched on Please contact your Agilent service with the top cover and foam removed representative 1290 Infinity TCC User Manual 95 6 Error Information Left Temperature Timeout Error ID 2811 The temperature of the left heat exchanger did not reach the temperature setpoint within the timeout threshold Probable cause Suggested actions 1 Defective left heater assembly Please contact your Agilent service representative 2 Defective TCC board Please contact your Agilent service representative Right Temperature Timeout Error ID 2812 The temperature of the right heat exchanger did not reach the temperature setpoint within the timeout threshold Probable
62. e holders must be avoided Some adjustments described in the manual are made with power supplied to the instrument and protective covers removed Energy available at many points may if contacted result in personal injury Any adjustment maintenance and repair of the opened instrument under voltage should be avoided whenever possible When inevitable this has to be carried out by a skilled person who is aware of the hazard involved Do not attempt internal service or adjustment unless another person capable of rendering first aid and resuscitation is present Do not replace components with power cable connected Do not operate the instrument in the presence of flammable gases or fumes Operation of any electrical instrument in such an environment constitutes a definite safety hazard Do not install substitute parts or make any unauthorized modification to the instrument Capacitors inside the instrument may still be charged even though the instrument has been disconnected from its source of supply Dangerous voltages capable of causing serious personal injury are present in this instrument Use extreme caution when handling testing and adjusting When working with solvents observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet by the solvent vendor especially when toxic or hazardous solvents are used 1290 Infinity TCC Use
63. e instrument housing and ensures the column is always kept at the setpoint temperature At 36 C the column setpoint and heat exchanger temperatures are equal temperature cross over point This is the temperature at which a calibrated measuring device can be used to calibrate the column thermostat Temperature temperature at difference C H measuring point Column setpoint temperature Calibration at cross over point 36 C Temperature C 80 Figure 24 1 Point Calibration at the Temperature Cross Over Point The column thermostat is calibrated correctly when the measured temperature using the external measuring device Temperature Calibration Procedure on page 108 and the cross over temperature 86 C of both heat exchangers left and right are within a range of 0 5 C 1290 Infinity TCC User Manual 107 7 Test Functions and Calibrations Temperature Calibration Procedure Tools required Parts required 108 Description Temperature measuring device see note below Description 1 Calibrated temperature measuring device For the measuring and calibration process Agilent Technologies recommends a thermometer with 0 1 C precision Contact the local Agilent Technologies support representative for ordering information The figures in this procedure refer to a specific type of temperature sensor Heraeus Quat340 quartz surface temperature measurement sensor Other sens
64. e outlet Figure 36 Possible Leak Areas 1290 Infinity TCC User Manual Maintenance 8 Replacing Head Parts of Column Switching Valve Replacing Head Parts of Column Switching Valve Figure 37 Valve Head Parts example shows a 5067 4159 valve head 1 Stator screws 2 Stator head assembly 3 Stator face assembly 4 Stator ring available for service only 5 Rotor seal 6 Bearing ring P N 1534 4045 7 Spanner nut P N 5068 0106 available for service only When If valve leaks Tools required Description Wrench 1 4 inch Hexagonal key 9 64 inch 1290 Infinity TCC User Manual 133 8 Maintenance Replacing Head Parts of Column Switching Valve NOTE Figure 37 on page 133 illustrates replacement parts for the valve heads with the 12Pos 13Port Selector valve as an example The valves can vary in their appearance and do not necessarily include all of the illustrated parts Neither every spare part is available for each flavor of the valve Use the tables Table 24 on page 153 Table 25 on page 154 for identification of the required part numbers 1 Remove capillaries from ports _ ps Or SA jae eT 2 Loosen each fixing stator screw two turns at a time Remove bolts from head L A Oe a Teor HH 0 Remove the stator head and stator face if applicable Remove the stator ring Remove the rotor seal and isolation seal if damaged or contaminated ao a A Ww
65. eak 91 leaks correcting 131 left fan failed 94 left temperature timeout 96 line frequency 27 line voltage 27 lithium batteries 202 lost CAN partner 90 M maintenance feedback 194 replacing firmware 143 materials bio inert 21 message remote timeout 90 Index non operating altitude 27 non operating temperature 27 0 open cover 95 operating Altitude 27 operating temperature 27 optimization 73 optimizing the performance 74 P packaging damaged 40 parts identification leak panels 147 performance Optimization 73 optimizing 74 specifications and features 28 physical specifications 27 27 power consideration 24 power consumption 27 power cords 25 power supply indicator 83 pre column heatexchanger 34 R radio interference 203 remote cable 168 repairs changing the column 117 column switching valve parts 133 correcting leaks 131 definition of 112 introduction 112 207 Index overview 115 replacing firmware 143 warnings and cautions 112 right fan failed 94 right temperature timeout 96 RS 232C cable 174 communication settings 191 S safety class 199 safety information lithium batteries 202 safety general information 199 standards 27 symbols 198 security lever 48 serial number information 182 shutdown 89 site requirements power cords 25 solvent information 36 sound emission 204 special interfaces 189 special settings boot resident 193 forced cold start 193 specificatio
66. ed fejlagtig handtering Udskiftning ma kun ske med batteri af samme fabrikat og type gt Lever det brugte batteri tilbage til leverand ren Lithiumbatteri Eksplosionsfare Ved udskiftning benyttes kun batteri som anbefalt av apparatfabrikanten gt Brukt batteri returneres appararleverandoren 202 Bij dit apparaat zijn batterijen geleverd Wanneer deze leeg zijn moet u ze niet weggooien maar inleveren als KCA 1290 Infinity TCC User Manual Appendix 12 Radio Interference Cables supplied by Agilent Technologies are screened to provide optimized protection against radio interference All cables are in compliance with safety or EMC regulations Test and Measurement If test and measurement equipment is operated with unscreened cables or used for measurements on open set ups the user has to assure that under operating conditions the radio interference limits are still met within the premises 1290 Infinity TCC User Manual 203 12 Appendix Sound Emission Manufacturer s Declaration This statement is provided to comply with the requirements of the German Sound Emission Directive of 18 January 1991 This product has a sound pressure emission at the operator position lt 70 dB e Sound Pressure Lp lt 70 dB A At Operator Position e Normal Operation e According to ISO 7779 1988 EN 27779 1991 Type Test 204 1290 Infinity TCC User Manual Appendix 12 Agilent Technologies on Internet For the
67. ent Layout The industrial design of the module incorporates several innovative features It uses Agilent s E PAC concept for the packaging of electronics and mechanical assemblies This concept is based upon the use of expanded polypropylene EPP layers of foam plastic spacers in which the mechanical and electronic boards components of the module are placed This pack is then housed in a metal inner cabinet which is enclosed by a plastic external cabinet The advantages of this packaging technology are e virtual elimination of fixing screws bolts or ties reducing the number of components and increasing the speed of assembly disassembly e the plastic layers have air channels molded into them so that cooling air can be guided exactly to the required locations the plastic layers help cushion the electronic and mechanical parts from physical shock and e the metal inner cabinet shields the internal electronics from electromagnetic interference and also helps to reduce or eliminate radio frequency emissions from the instrument itself 1290 Infinity TCC User Manual 195 11 Hardware Information Instrument Layout 196 1290 Infinity TCC User Manual 1290 Infinity TCC User Manual 12 Appendix General Safety Information 198 The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC 201 Lithium Batteries Information 202 Radio Interference 203 Sound Emission 204 Agilent Technologies on Internet 205 This chap
68. equires a HOST module with on board LAN e g G4212A or G4220A with minimum firmware B 06 40 or C 06 40 or with ad ditional G1369C LAN Card The detector DAD MWD FLD VWD RID is the preferred access point for control via LAN The inter module communication is done via CAN e CAN connectors as interface to other modules e LAN connector as interface to the control software e RS 232C as interface to a computer e REMOTE connector as interface to other Agilent products e Analog output connector s for signal output 1290 Infinity TCC User Manual 185 11 Hardware Information 186 Overview Interfaces CAN The CAN is inter module communication interface It is a 2 wire serial bus system supporting high speed data communication and real time requirement LAN The modules have either an interface slot for an LAN card e g Agilent G1369B C LAN Interface or they have an on board LAN interface e g detectors G1315C D DAD and G1365C D MWD This interface allows the control of the module system via a PC with the appropriate control software Some modules have neither on board LAN nor an interface slot for a LAN card e g G1170A Valve Drive or G4227A Flex Cube These are hosted modules and require a Host module with firmware B 06 40 or later or with additional G1369C LAN Card If an Agilent detector DAD MWD FLD VWD RID is in the system the LAN should be connected to the DAD MWD FLD VWD RID due to higher data load If no Agilent de
69. es 171 CAN Cable 173 RS 232 Cable Kit 174 Agilent Module to Printer 175 1 Hardware Information 177 Firmware Description 178 Electrical Connections 181 Interfaces 184 Setting the 8 bit Configuration Switch without On board LAN 190 Early Maintenance Feedback 194 Instrument Layout 195 12 Appendix 197 General Safety Information 198 The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC 201 Lithium Batteries Information 202 Radio Interference 203 Sound Emission 204 Agilent Technologies on Internet 205 1290 Infinity TCC User Manual Contents 8 1290 Infinity TCC User Manual 1290 Infinity TCC User Manual 1 Introduction to the Column Compartment Overview of the Module 10 System Overview 11 Column ldentification System 13 Column Switching Valve Optional 15 Typical Applications 18 Bio inert Materials 21 This chapter gives an introduction to the TCC and an instrument overview Apg Agilent Technologies 1 Introduction to the Column Compartment Overview of the Module The Agilent 1290 Infinity Thermostatted Column Compartment is a stackable temperature controlled column compartment for LC It is available as standalone module or as a component of an Agilent 1290 Infinity system It is used for heating and cooling to meet extreme requirements of retention time reproducibility The main features of the 1290 Infinity Thermostatted Column Compartment are Peltier heating a
70. evelopment System System Manual G4230 90001 and in the Agilent G4231B and G4232B Valve Kit Instructions G4232 90003 Table 22 Heater Devices Item Description Q gt QS 2 7 za a NS gn e gt iP QS Q LA Heater long up 0 12 mm i d 1 6 uL internal volume G1316 80002 Part of Capillary kit 5067 4646 For fittings see Table 23 on page 150 Heat Exchanger Long Up 0 075 mm nom Volume 1 0 uL G1316 80012 Heater long down 0 12 mm i d 1 6 uL internal volume G1316 80003 Part of Capillary kit 5067 4646 For fittings see Table 23 on page 150 Heat Exchanger Long Down 0 075 mm nom 1 0 uL G1316 80013 Column clip set 8 colors 5042 9918 148 1290 Infinity TCC User Manual Parts and Materials for Maintenance 9 Table 22 Heater Devices Item Description Carrier for heat exchanger TCC SL Plus G1316 89200 Fitting holder assembly G1316 68706 amp fi no LG ry Rate i CESS a y N lt e Th s Bio Inert Solvent Heating Devices 9 pL internal a i volume 600 bar max pressure G5616 60050 1290 Infinity TCC User Manual 149 9 Parts and Materials for Maintenance Table 23 TCC Heater Fittings Item Description 9 Plastic fittings 0100 1259 7 Very high pressure removable fitting 5067 4738 8 Fitting screw long 5 Ferrule back 6 Ferrule front 1 included in kit Fitting screw long 5065 4454 D Gy eS oh
71. ferent power cords are offered as options with the module The female end of all power cords is identical It plugs into the power input socket at the rear The male end of each power cord is different and designed to match the wall socket of a particular country or region Absence of ground connection or use of unspecified power cord The absence of ground connection or the use of unspecified power cord can lead to electric shock or short circuit gt Never operate your instrumentation from a power outlet that has no ground connection gt Never use a power cord other than the Agilent Technologies power cord designed for your region Use of unsupplied cables Using cables not supplied by Agilent Technologies can lead to damage of the electronic components or personal injury gt Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Unintended use of supplied power cords Using power cords for unintended purposes can lead to personal injury or damage of electronic equipment gt Never use the power cords that Agilent Technologies supplies with this instrument for any other equipment 1290 Infinity TCC User Manual 25 2 Site Requirements and Specifications Bench Space The module dimensions and weight see Table 3 on page 27 allow you to place the module on almost any desk or laboratory bench It needs an additional 2 5 cm
72. gement even without the autosampler thermostat A slightly longer capillary is required between the pump and autosampler See Figure 11 on page 44 and Figure 12 on page 45 Instant Pilot Detector Column compartment ANIMA UUNIT Solvent cabinet b Pump Autosampler Thermostat for the ALS optional Figure 11 Recommended two stack configuration for 1290 Infinity with binary pump front view 44 1290 Infinity TCC User Manual Installing the Module 3 Optimizing the Stack Configuration LAN to LC ChemStation CAN Bus cable to Instant Pilot Analog detector signal tional optional EN Ul PA CAN Bus cable Thermo cable optional AC Power Figure 12 Recommended two stack configuration for 1290 Infinity with binary pump rear view 1290 Infinity TCC User Manual 45 3 Installing the Module Installing the Column Compartment Parts required Preparations CAUTION Description 1 Column compartment 1 Power cord for other cables see text below Locate bench space Provide power connections Unpack the column compartment Defective on arrival problems If there are signs of damage please do not attempt to install the module Inspection by Agilent is required to evaluate if the instrument is in good condition or damaged gt Notify your Agilent sales and service office about the damage gt An Agilent service representative will inspec
73. h connection is being made Agilent Module to 3394 6 Integrators p n 35900 60750 Pin 3394 6 Pin Agilent Signal Name module 1 Not connected 2 Shield Analog 3 Center Analog Agilent Module to BNC Connector p n 8120 1840 Pin BNC Pin Agilent Signal Name module Shield Shield Analog an Center Center Analog 166 1290 Infinity TCC User Manual Agilent Module to General Purpose Identifying Cables 10 p n 01046 60105 Pin Agilent Signal Name module Not connected Black Analog ES Red Analog a 1290 Infinity TCC User Manual 167 10 Identifying Cables Remote Cables 168 One end of these cables provides a Agilent Technologies APG Analytical Products Group remote connector to be connected to Agilent modules The other end depends on the instrument to be connected to Agilent Module to 3396A Integrators p n 03394 60600 Pin 3396A Pin Agilent Signal Name Active module TTL 9 1 White Digital ground NC 2 Brown Prepare run Low 80 A 3 3 Gray Start Low z NC 4 Blue Shut down Low e oa NC 5 Pink Not Ls connected NC 6 Yellow Power on High 5 14 7 Red Ready High 1 8 Green Stop Low NC 9 Black Start request Low 13 15 Not connected Agilent Module to 3396 Series II 3395A Integrators Use the cable Agilent module to 3396A Series I integrators 03394 60600 and cut pin 5 on the integrator
74. he Agilent 1200 Infinity Series 2 position 10 port valves can be used to increase the efficiency in laboratories running large amounts of samples Gradient elution is a common technique for separation of complex samples in liquid chromatography which requires column regeneration before the subsequent run is started Using alternating column regeneration valuable time for the analysis is saved Core of the alternating column regeneration is the Agilent 1200 Infinity Series 2 position 10 port valves which allows simultaneous analysis on one column while a second identical column is flushed and equilibrated Column 2 Column 2 Eluent pump Autosampler Regeneration pump Position 2 With sequential Analysis 1 Regene Analysis 2 Regene Analysis 3 regeneration ze OO eee OO ee o O ay re With alternating Analysis 1 Regene Analysis 3 regeneration as Analysis 2 Regene Figure 7 Alternate Column Regeneration Time Scheme 1290 Infinity TCC User Manual Introduction to the Column Compartment 1 Bio inert Materials For the Agilent 1260 Infinity Bio inert LC system Agilent Technologies uses highest quality materials in the flow path also referred to as wetted parts which are widely accepted by life scientists as they are known for optimum inertness to biological samples and ensure best compatibility with common samples and solvents over a wide pH range Explicitly the complete flow path is free of s
75. he module If the delivery packaging shows signs of external damage please call your Agilent Technologies sales and service office immediately Inform your service representative that the instrument may have been damaged during shipment CAUTION Defective on arrival problems If there are signs of damage please do not attempt to install the module Inspection by Agilent is required to evaluate if the instrument is in good condition or damaged gt Notify your Agilent sales and service office about the damage gt An Agilent service representative will inspect the instrument at your site and initiate appropriate actions Delivery Checklist Table 19 Column Compartment Delivery Checklist Description Quantity Thermostatted column compartment 1 Power cable 1 CAN cable 1 Column switching valve optional User Manual on User Documentation DVD 1 Accessory kit see Accessory Kits on 1 page 155 40 1290 Infinity TCC User Manual Installing the Module 3 Optimizing the Stack Configuration If your column compartment is part of an Agilent 1290 Infinity system you can ensure optimum performance by installing the following configuration This configuration optimizes the system flow path ensuring minimum delay volume For installations of the G1316C as part of the Method Development Solution please refer to the Agilent 1200 Infinity Series Method Development System System Manual G4230 90002 1290 Infinity TCC User M
76. her analytical instruments from Agilent Technologies if you want to use features as common shut down prepare and so on Remote control allows easy connection between single instruments or systems to ensure coordinated analysis with simple coupling requirements The subminiature D connector is used The module provides one remote connector which is inputs outputs wired or technique To provide maximum safety within a distributed analysis system one line is dedicated to SHUT DOWN the system s critical parts in case any module detects a serious problem To detect whether all participating modules are switched on or properly powered one line is defined to summarize the POWER ON state of all connected modules Control of analysis is maintained by signal readiness READY for next analysis followed by START of run and optional STOP of run triggered on the respective lines In addition PREPARE and START REQUEST may be issued The signal levels are defined as e standard TTL levels 0 V is logic true 5 0 V is false fan out is 10 e input load is 2 2 kOhm against 5 0 V and e output are open collector type inputs outputs wired or technique All common TTL circuits operate with a 5 V power supply A TTL signal is defined as low or Lwhen between 0 V and 0 8 V and high or H when between 2 0 V and 5 0 V with respect to the ground terminal 1290 Infinity TCC User Manual Hardware Information 11 Table 28 Remote Signal Distributi
77. hough this will have an effect on the chromatography This effect becomes more significant at low flow rates and small column volumes and can have a large impact on the transferability of gradient methods It is important therefore for fast gradient separations to have small delay volumes especially with narrow bore columns e g 2 1 mm i d as often used with mass spectrometric detection The delay volume in a system includes the volume in the pump from the point of mixing connections between pump and autosampler volume of the flow path through the autosampler and connections between autosampler and column 76 1290 Infinity TCC User Manual How to optimize the Column Compartment 4 Extra Columwn Volume Extra column volume is a source of peak dispersion that will reduce the resolution of the separation and so should be minimized Smaller diameter columns require proportionally smaller extra column volumes to keep peak dispersion at a minimum In a liquid chromatograph the extra column volume will depend on the connection tubing between the autosampler column and detector and on the volume of the flow cell in the detector The extra column volume is minimized with the Agilent 1290 Infinity Agilent 1260 Infinity LC system due to the narrow bore 0 12 mm i d tubing the low volume heat exchangers in the column compartment and the Max Light cartridge cell in the detector 1290 Infinity TCC User Manual 71 4 How to optimize the Colum
78. iddle position In case you are going to use columns of maximum 100 mm length it is advised to place two carriers with one heat exchanger each at the left and the right side of the column compartment see Figure 29 on page 120 To use the column tag readers the carriers must be installed either in the top or bottom positions The carriers for the low dispersion heat exchangers must be attached to the standard built in heat exchangers of the TCC see Figure 30 on page 122 Remove the protective foil from the gray thermal conductive foil of the carrier and fasten the three screws Mount the fitting holder assembly Fitting holder assy includes following items Fitting fork fitting clip screws pack of 4 G1316 68706 on the carrier The fitting clips hold the capillary unions from the low dispersion heat exchangers and make plumbing of capillaries much easier Finally attach the low dispersion heat exchanger It is important to fix them tightly so that a good thermal conductivity is achieved The columns are held by color coded clips Column clip set 8 colors 5042 9918 for more convenient installation mark the nuts of the capillaries attached to the columns as well with color code tags small rings to easily follow the flow paths in your system 1290 Infinity TCC User Manual 121 8 Maintenance Adding Heater Devices Fixing the Low Dispersion Heat Exchangers For the G1316C additional heat exchanger devices can be installed on the
79. ient temperature 25 C set point at 40 C and a flow range from 0 2 5 ml min 1290 Infinity TCC User Manual Valve Specifications Site Requirements and Specifications 2 Table5 G4230A Method Dev Valve Kit 8 Pos 9 port 600 bar Type Specification Liquid contacts Port size Flow passage diameters Port to Port Volume Maximum pressure Comments PEEK Stainless Steel Accepts 10 32 male threaded fittings Stator 0 28 mm 0 011 in Rotor Seal 0 31 mm 0 012 in 2 21 uL 600 bar Kit contains 2x 8 Pos 9 port 600 bar valve heads Table6 G4230B Method Dev Valve Kit 8 Pos 9 port 1200 bar Type Specification Liquid contacts Port size Flow passage diameters Port to Port Volume Maximum pressure Comments Vespel Stainless Steel Accepts 10 32 male threaded fittings Stator 0 28 mm 0 011 in Rotor Seal 0 31 mm 0 012 in 0 55 pL 1200 bar Kit contains 1x 8 Pos 9 port 1200 barr and 1 x 8 Pos 9 port 600 bar valve head Table 7 4231A Agilent 1290 Series 2 Position 6 Port valve head 600bar Type Specification Liquid contacts Port size Flow passage diameters Port to Port Volume Maximum pressure PEEK Stainless Steel Accepts 10 32 male threaded fittings Stator 0 38 mm 0 015 in Rotor Seal 0 30 mm 0 012 in 0 51 pL 600 bar 1290 Infinity TCC User Manual 29 2 Site Requirements and Specifications Table 8 G423
80. illaries Configuration for sample clean up Figure 34 Installing the capillaries for a sample clean up set up 1 Analytical column with color code clip 2 280 mm length column length gt 100 mm from analytical column to detector Not pre swaged on column side 3 340 mm length from autosampler and loading pump 150 mm length column length up to 100 mm 280 mm length column length gt 100 mm From column Not pre swaged on column side 5 Pre column with color code clip Low dispersion heat exchanger or build in 3 pL 6 uL heat exchanger and 90 mm length capillary to column 7 90 mm length to heat exchanger 8 700 mm length 0 17 mm ID from analytical pump 9 To waste 10 90 mm length to heat exchanger 11 Low dispersion heat exchanger or build in 3 pL 6 pL heat exchanger and 90 mm length capillary to column Pos 1 Connection between ports 1 6 4 5 2 3 active column 1 left Pre column Pos 2 Connection between Ports 1 2 3 4 5 6 active column 2 right analytical column 1290 Infinity TCC User Manual 129 Maintenance Configuration for alternating column regeneration 130 Figure 35 Installing the capillaries for alternating column regeneration column and heat exchanger of the second position are omitted 10 11 Valve Valve connector 120 mm length 150 mm length column length up to 100 mm 280 mm length column length gt 100 mm From column Not pre swaged on column side 200 mm length t
81. implemented differently For example for a pump the flow will be stopped for safety reasons For a detector the lamp will stay on in order to avoid equilibration time Depending on the error type the next run can only be started if the error has been resolved for example liquid from a leak has been dried Errors for presumably single time events can be recovered by switching on the system in the user interface Special handling is done in case of a leak As a leak is a potential safety issue and may have occurred at a different module from where it has been observed a leak always causes a shutdown of all modules even outside a method run In all cases error propagation is done via the CAN bus or via an APG remote cable see documentation for the APG interface 1290 Infinity TCC User Manual General Error Messages Error Information 6 General error messages are generic to all G HPLC modules Error ID 0062 The timeout threshold was exceeded Probable cause 1 The analysis was completed successfully and the timeout function switched off the module as requested 2 Anot ready condition was present during a sequence or multiple injection run for a period longer than the timeout threshold Shutdown Error ID 0063 Suggested actions Check the logbook for the occurrence and source of a not ready condition Restart the analysis where required Check the logbook for the occurrence and source of a not ready condition
82. led 1290 Infinity TCC User Manual Introduction to the Column Compartment Column Switching Valve Optional Column Switching Valve Optional 1 Figure 3 Location of Column Switching Valve 1290 Infinity TCC User Manual 15 1 16 Introduction to the Column Compartment Two Column Selection The valve can select either column 1 or column 2 The offline column is sealed by connecting head to tail Switching should be done when the flow is off and the pressure is zero Before switching the valve switch off the pump or set the flow to zero Keeping the flow on while the valve is switched can cause exceeding the maximum pressure This will stop method or sequence execution From autosampler Heater assembly 1 Heater assembly 2 To detector Figure 4 Column 1 Active From autosampler Heater assembly 1 Heater assembly 2 Figure 5 Column 2 Active 1290 Infinity TCC User Manual Introduction to the Column Compartment 1 Precolumn Back flushing The sample is injected into series connected precolumn and analytical column After the valve has switched the analytical column flow continues in normal direction Only the precolumn is back flushed eluting highly retained peaks directly to the detector From autosampler Heater assembly 1 Heater assembly 2 Analytical Column To detector Figure 6 Precolumn Back flushing 1290 Infinity TCC User Manual 17
83. m CAUTION paisa i dust and other pollutions Pollution of these parts can impair the accurate selection of valve ports and therefore bias measurement results gt Always install a valve head for operation and storage For protecting the actuator a dummy valve head part of Transportation Lock Kit G1316 67001 can be used instead of a functional valve Do not touch parts inside the actuator The tag reader reads the valve head properties from the valve head RFID tag during initialization of the module Valve properties will not be updated if the valve head is replaced while the module is on Selection of valve port positions can fail if the instrument does not know the properties of the installed valve To have the valve correctly recognized by the Agilent Infinity Valve Drive you must have the valve drive powered off for at least 10 seconds 1290 Infinity TCC User Manual 137 8 Maintenance Replacing Valve Heads 1 Switch off the module 4 Unscrew the valve head 2 Push the valve head for bringing it to its outer position TCC only 3 Remove all capillary connections from the valve head 5 Put the new valve head onto the valve drive such that the 6 Screw the valve head onto the valve drive using the union lobe fits to the groove see also Installing the Valve nut see also Installing the Valve Heads on page 50 Heads on page 50 138 1290 Infinity TCC User Manual Maintenance
84. mn Compartment 73 Optimizing the Column Compartment 74 Using Additional Heater Devices 75 Delay Volume and Extra Column Volume 76 How to Configure the Optimum Delay Volume 78 How to Achieve Higher Injection Volumes 79 How to Achieve High Throughput 80 1290 Infinity TCC User Manual Contents 5 Troubleshooting and Diagnostics 81 Overview of the Module s Indicators and Test Functions 82 Status indicators 83 Available Tests vs User Interfaces 85 Agilent Lab Advisor Software 86 6 Error Information 87 What Are Error Messages 88 General Error Messages 89 TCC Error Messages 93 7 Test Functions and Calibrations 103 Thermostat Function Test 104 Pressure Test 106 Temperature Calibration 107 8 Maintenance 111 Introduction to Maintenance and Repair 112 Cautions and Warnings 113 Overview of Maintenance 115 Cleaning the Column Compartment 116 Changing Column Identification Tags 117 Adding Heater Devices 119 Installing the capillaries 124 Correcting Leaks 131 Replacing Head Parts of Column Switching Valve 133 Replacing Valve Heads 136 Preparing the Column Compartment for Transportation 140 Replacing the Module Firmware 143 9 Parts and Materials for Maintenance 145 Plastic Parts 146 Leak Parts 147 Heater and Cooling Devices 148 Valve Options Overview 152 Accessory Kits 155 1290 Infinity TCC User Manual Contents 10 Identifying Cables 163 Cable Overview 164 Analog cables 166 Remote Cables 168 BCD Cabl
85. mply with local regulatory requirements calibration and verification may be required The following sections describe these functions in detail 82 1290 Infinity TCC User Manual Troubleshooting and Diagnostics 5 Status indicators Two status indicators are located on the front of the module The lower left indicates the power supply status the upper right indicates the module status Status indicator green yellow red Line power switch with green light Figure 22 Location of Status indicators Power Supply Indicator The power supply indicator is integrated into the main power switch When the indicator is illuminated green the power is ON 1290 Infinity TCC User Manual 83 5 Troubleshooting and Diagnostics Module Status Indicator The module status indicator indicates one of six possible module conditions 84 When the status indicator is OFF and power switch light is on the module is ina prerun condition and is ready to begin an analysis A green status indicator indicates the module is performing an analysis run mode A yellow indicator indicates a not ready condition The module is ina not ready state when it is waiting for a specific condition to be reached or completed for example immediately after changing a set point or while a self test procedure is running An error condition is indicated when the status indicator is red An error condition indicates the module has
86. n physical 27 specifications 28 status indicator 84 system overview 11 T tag installation 70 temperature calibration 208 description 107 problems 109 procedure 108 temperature sensor 91 temperature verification external measuring device 108 principle 109 temperature range 28 test thermostat function test result 104 thermostat function test 82 timeout 89 troubleshooting error messages 82 88 status indicators 82 83 U unknown unsupported valve detected 100 user interfaces 85 V valve failed to initialize 100 valve RFID access failure 101 verification temperature 82 voltage range 27 wW warm up time 28 waste electrical and electronic equipment 201 WEEE directive 201 weight 27 1290 Infinity TCC User Manual www agilent com In This Book The manual contains technical reference information about the Agilent 1290 Infinity Thermostatted Column Compartment This manual describes the following e introduction and specifications e installation using and optimizing e troubleshooting and diagnose e maintenance parts identification e safety and related information Agilent Technologies 2008 2009 2011 2012 2013 Printed in Germany 02 2013 G1316 90032 fe Agilent Technologies
87. n Compartment How to Configure the Optimum Delay Volume To minimize peak dispersion and delay volume in the thermostatted column compartment the low dispersion heat exchanger must be installed The low dispersion heat exchanger is part of the capillary kits recommended for low dispersion applications The common capillary kit also includes narrow 0 12 mm i d capillaries The built in 3 ul and 6 ul heat exchangers are for backwards compatibility and should only be used in case a conventional method needs to be run on the system even though in this case also the low dispersion heat exchanger could be used 78 1290 Infinity TCC User Manual How to optimize the Column Compartment 4 How to Achieve Higher Injection Volumes One way to achieve larger injections is to use a trapping column selected by a switching valve to capture and concentrate the injection before switching it i e injecting it onto an analytical column see Sample Enrichment on page 79 The valve can be conveniently located in the thermostatted column compartment or in the flexible cube Sample Enrichment Pump A autosampler Waste Enrichment column Position 1 1290 Infinity TCC User Manual Analytical column detector Pump A autosampler Analytical column detector Enrichment column Position 2 79 4 How to optimize the Column Compartment How to Achieve High Throughput The column equilibration step can be a significant part of
88. n defined limits If the current increases above the upper limit the error message is generated Probable cause Suggested actions 1 Defective leak sensor Please contact your Agilent service representative 2 Leak sensor incorrectly routed being e Please contact your Agilent service pinched by a metal component representative e Please contact your Agilent service representative 1290 Infinity TCC User Manual Error Information 6 TCC Error Messages These errors are module specific Compensation Sensor Open Error ID 0081 The ambient compensation sensor NTC on the main board in the module has failed open circuit The resistance across the temperature compensation sensor NTC on the main board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor increases above the upper limit the error message is generated Probable cause Suggested actions 1 Defective main board Please contact your Agilent service representative Compensation Sensor Short Error ID 0080 The ambient compensation sensor NTC on the main board in the module has failed short circuit The resistance across the temperature compensation sensor NTC on the main board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across
89. n the valve head until the outside pin completely locks in While turning the valve you should feel some resistance from the drive Otherwise the pins on the valve shaft are not properly fitted into the corresponding adapters on the valve drive It can be possible that you have to turn the valve head until the outside pin completely locks in While turning the valve you should feel some resistance from the drive Otherwise the pins on the valve shaft are not properly fitted into the corresponding adapters on the valve drive 54 1290 Infinity TCC User Manual Installing the Module Installing Valve Heads 3 3 When the outer pin is locked into the groove manually screw the nut onto the valve head Fasten the nut manually Do not use any tools 4 Install all required capillary connections to the valve 5 Push the valve head until it snaps in and stays in the rear position TCC only 6 Power on or power cycle your module so the valve head gets recognized during module initialization NOTE Power Off the Infinity valve drive for at least 10 s 1290 Infinity TCC User Manual 55 3 Installing the Module Installation of Heater Devices Availability of heater devices Additional to the built in heat exchangers external heater devices with a small internal volume for low dispersion applications are available The delivered low dispersion heat exchanger should always be used to minimize
90. nd cooling from 10 degrees below ambient up to 100 C with high heating and cooling speeds for maximum application flexibility and stability holds up to three 30 cm columns optimized design gives minimum dead volumes and maximum efficiency two independently programmable heat exchangers contribute volumes of only 3 and 6 ul additional heating devices for low flow rates which reduce the risk of additional dispersion it can be supplemented by a kit to install a small heat exchanger with 1 6 ul delay volume to reduce the delay volume electronic column identification as standard for GLP documentation of column type and major column parameters optional high quality Quick Change Valve Heads For specifications see Performance Specifications on page 28 10 1290 Infinity TCC User Manual Introduction to the Column Compartment 1 System Overview The Concept of Heating and Cooling The design of this thermostatted column compartment uses column heating and cooling devices with Peltier elements The solvent entering the column compartment is heated up or cooled down to a settable temperature with two low volume heat exchangers 3 ul on left side 6 ul on right side made of a short piece of capillary 0 17 mm i d leading through a heat exchanger The heat exchanger is designed such that it can function simultaneously as an air heater The shape of the heat exchanger surface allows the area around the column to be kept at
91. nitors the usage of specific components in the instrument and provides feedback when the user selectable limits have been exceeded The visual feedback in the user interface provides an indication that maintenance procedures should be scheduled EMF Counters EMF counters increment with use and can be assigned a maximum limit which provides visual feedback in the user interface when the limit is exceeded Some counters can be reset to zero after the required maintenance procedure Using the EMF Counters The user settable EMF limits for the EMF Counters enable the early maintenance feedback to be adapted to specific user requirements The useful maintenance cycle is dependent on the requirements for use Therefore the definition of the maximum limits need to be determined based on the specific operating conditions of the instrument Setting the EMF Limits The setting of the EMF limits must be optimized over one or two maintenance cycles Initially the default EMF limits should be set When instrument performance indicates maintenance is necessary take note of the values displayed by the EMF counters Enter these values or values slightly less than the displayed values as EMF limits and then reset the EMF counters to zero The next time the EMF counters exceed the new EMF limits the EMF flag will be displayed providing a reminder that maintenance needs to be scheduled 1290 Infinity TCC User Manual Hardware Information 11 Instrum
92. nless steel cladded PEEK capillaries gt Follow the instructions given in the technical note Installation of Stainless Steel Cladded PEEK Capillaries G5611 90120 1290 Infinity TCC User Manual 59 3 Installing the Module Flow Connections of the Column Compartment Flow Connections Using Low Dispersion Heat Exchangers Parts required Description Other modules Parts from Accessory Kit Two wrenches 1 4 5 16 inch for capillary connections Preparations Install the column compartment Install the additional heat exchangers WARNING Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and reagents can hold health and safety risks gt When working with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice gt The volume of substances should be reduced to the minimum required for the analysis gt Do not operate the instrument in an explosive atmosphere 1 Press release buttons and remove front cover to gain 2 Place the column on the left heat exchanger assembly access to heater area and connect the capillaries to the column From autosampler To detector 60 1290 Infinity TCC User Manual Installing the Module 3 Flow Connections
93. o detector 150 mm length column length up to 100 mm 280 mm length column length gt 100 mm From column Not pre swaged on column side Column with color code clip Low dispersion heat exchanger or build in 3uL 6uL heat exchanger and 90 mm length capillary to column 90 mm length to heat exchanger To waste From autosampler 700 mm length 0 17 mm ID from regeneration pump 90 mm length to heat exchanger Pos 1 Connection between ports 1 10 2 3 4 5 6 7 8 9 active column 1 left regemerating column right Pos 2 Connection between ports 1 2 3 4 5 6 7 8 9 10 active column 2 right regenerating column left 1290 Infinity TCC User Manual Maintenance 8 Correcting Leaks When Tools required If a leakage has occurred at the heat exchanger or at the capillary connections or at the column switching valve Description Tissue Pipette Wrench 1 4 5 16 inch for capillary connections Depending on the column position or the use of additional heat exchanger assemblies the view of Figure 36 on page 132 may vary 1 Remove the front cover 2 Use a pipette and tissue to dry the leak sensor area 3 Observe the capillary connections and the column switching valve for leaks and correct if required 1290 Infinity TCC User Manual 131 132 8 Maintenance Correcting Leaks 4 Re install the front cover Column switching valve Column Leak sensor assembly Wast
94. o inert 154 1290 Infinity TCC User Manual Parts and Materials for Maintenance 9 Accessory Kits The accessory kits contain accessories and tools needed for installation and maintenance Standard Accessory Kit The standard accessory kit is always delivered with the instrument You might want to order one of the following items if you choose to re install the instrument at a later time Accessory kit G1316 68765 p n Description 5063 6527 Tubing assembly i d 6 mm o d 9 mm 1 2 m to waste 5181 1516 CAN cable Agilent module to module 0 5 m G1316 01202 2x Column clamp G1316 87300 Capillary 0 17 x 90 mm 1 16 in male male G1316 87321 Capillary column heat exchanger 105 mm lg 0 17 mm i d G1316 87323 Capillary column heat exchanger 170 mm lg 0 17 mm i d 1290 Infinity TCC User Manual 155 9 Parts and Materials for Maintenance Low Dispersion Capillary Kit for G1316C Capillary Kit 5067 4633 p n Description 0100 0043 ST front ferrule 0100 0044 ST back ferrule 0100 2086 Nut seal tight pack of 2 5021 1822 Flexible tubing 280 mm G1156 22401 Fitting Screw long G1316 68706 Fitting holder assy includes following items Fitting fork fitting clip screws pack of 4 G1316 89200 Carrier for heat exchanger G1316 80003 Heater long down 0 12 mm i d 1 6 uL internal volume 156 1290 Infinity TCC User Manual Parts and Materials for Maintenance List of Capillary and Column Kits for Method Development Sy
95. of the Column Compartment 3 Or place the column on the right heat exchanger assembly and connect the capillaries to the column See Column Switching Valve Optional on page 15 on how to connect the column selection valve 4 Fix the column with the column clip from the accessory kit 5 If the column compartment is not part of an Agilent LC system or if an Agilent autosampler is located on top connect the corrugated tubing to the waste outlet 6 Route tubings from modules above through the openings in the funnel holder top and the plastic bottom part Remove small plastic plugs first 1290 Infinity TCC User Manual 61 3 Installing the Module Flow Connections of the Column Compartment 7 Put the front cover back in place The installation of the column compartment has now been completed NOTE Always operate the TCC with the front cover in place for proper thermostatting conditions and to protect the column area against strong drafts from the ouside 62 1290 Infinity TCC User Manual Installing the Module 3 Flow Connections Using the Built in Heat Exchangers For applications that are not critical regarding very low dispersion volumes e g when using 4 6 mm i d columns the built in heat exchangers may be used Description Parts required 1 Other modules 1 1 Parts from Accessory Kit Two wrenches
96. on Pin Signal Description 1 DGND Digital ground 2 PREPARE L Request to prepare for analysis for example calibration detector lamp on Receiver is any module performing pre analysis activities 3 START L Request to start run timetable Receiver is any module performing run time controlled activities 4 SHUT DOWN L System has serious problem for example leak stops pump Receiver is any module capable to reduce safety risk 5 Not used 6 POWER ON H All modules connected to system are switched on Receiver is any module relying on operation of others 7 READY H System is ready for next analysis Receiver is any sequence controller 8 STOP L Request to reach system ready state as soon as possible for example stop run abort or finish and stop injection Receiver is any module performing run time controlled activities 9 START REQUEST L Request to start injection cycle for example by start key on any module Receiver is the autosampler Special Interfaces There is no special interface for this module 1290 Infinity TCC User Manual 189 11 Hardware Information Setting the 8 bit Configuration Switch without On board LAN 190 The 8 bit configuration switch is located at the rear of the module This module does not have its own on board LAN interface It can be controlled through the LAN interface of another module and a CAN connection to that module re SS Y Figure 46 Config
97. on acids bases and solvents There are no documented incompatibilities for HPLC applications Sapphire Ruby and Al 03 based ceramics Sapphire ruby and ceramics based on AlgOg are inert to almost all common acids bases and solvents There are no documented incompatibilities for HPLC applications Data above were collected from external resources and are meant as a reference Agilent cannot guarantee the completeness and correctness of such information Information can also not be generalized due to catalytic effects of impurities like metal ions complexing agents oxygen etc Most data available refers to room temperature typically 20 25 C 68 77 F If corrosion is possible it usually increases at higher temperatures If in doubt consult additional resources 1290 Infinity TCC User Manual 1290 Infinity TCC User Manual 3 Installing the Module Unpacking the module 40 Damage tothe module 40 Delivery Checklist 40 Optimizing the Stack Configuration 41 One Stack Configuration 42 Two Stack Configuration 44 Installing the Column Compartment 46 Installing Valve Heads 50 Installation of Heater Devices 56 Flow Connections of the Column Compartment 60 Installing Capillaries 67 Placing Columns 70 This chapter provides information on unpacking checking on completeness stack considerations and installation of the module Apg Agilent Technologies 39 3 Installing the Module Unpacking the module Damage to t
98. onnect the capillaries to the column From autosampler 4 Orplace the column on the right heat exchanger assembly and connect the capillaries to the column Optionally place the column tag in front of the tag reader To detector NOTE See Column Switching Valve Optional on page 15 on how to connect the column selection valve 5 Fix the column with the column clip from the accessory kit Column clip 6 Ifthe column compartment is not part of an Agilent LC system or if an Agilent autosampler is located on top connect the corrugated tubing to the waste outlet 1290 Infinity TCC User Manual 65 3 Installing the Module Flow Connections of the Column Compartment 7 Route tubings from modules above through the openings in the funnel holder top and the plastic bottom part Remove small plastic plugs first 8 Put the front cover back in place The installation of the column compartment has now been completed NOTE Always operate the TCC with the front cover in place for proper thermostatting conditions and to protect the column area against strong drafts from the outside 66 1290 Infinity TCC User Manual Installing the Module 3 Installing Capillaries Installing Capillaries NOTE For installation of stainless steel cladded PEEK capillaries used in modules delivered before January
99. ors may require a different fixing 1 Install the temperature sensor Installing the Temperature Sensor on page 109 2 Select the Column Compartment Temperature Calibration mode in the user interface 3 Wait for the temperature to stabilize at the calibration temperature 36 C Measure the temperature of the heat exchanger 5 Ifthe measured temperature deviates by more than 0 5 C from the actual temperature enter the measured value in the measured temperature field for the left heat exchanger 6 Install the sensor at the measurement point on the right heat exchanger Repeat the calibration procedure for the right heat exchanger Limits After calibration the measured temperature and the calibration temperature should be within 0 5 C The maximum deviation which can be adjusted is 1 6 C If the measured value and the calibration value differ by more than 1 6 C this is an indication that a problem exists Calibration Problems on page 109 1290 Infinity TCC User Manual Test Functions and Calibrations 7 Calibration Problems If the temperature cannot be calibrated check the following e Has the thermostat front cover been closed correctly Is the measuring device functioning correctly and is calibrated according to the manufacturers instructions Hardware Failures Probable hardware failures that can lead to a failed calibration procedure are Defective or wrongly calibrated measuring device
100. os 6port at port 2 instead Connect port 1 and port 8 with a 120 mm length capillary 0 12 mm i d or 0 17 mm i d depending on the capillary kit 6067 4652 Plug Plastic fittings 0100 1259 into ports 9 and 10 Plastic fitting length p n Description 1 5067 4646 Capillary kit 1 G4231B 2pos 6port valve 1 G4232B 2pos 10port valve Identify the required capillaries in your capillary kits Capillary kit PN 5067 4646 on page 158 Use outmost care to avoid any void volumes caused by poor connections 1290 Infinity TCC User Manual Maintenance 8 1 Install the capillaries depending on your application Dual column selection see Configuration for dual column selection on page 127 Sample Enrichment see Configuration for sample enrichment on page 128 Sample Clean up see Configuration for sample clean up on page 129 Alternating Column Regeneration only 2pos 10port valve see Configuration for alternating column regeneration on page 130 Use outmost care to avoid any void volumes caused by poor connections 2 Connect the capillaries connected directly to a column and fasten them immediately with a spanner Finger tighten all remaining capillaries 4 Clip the unions into the corresponding clips of the low dispersion heat exchangers 5 Fasten all fittings with a spanner Starting from position one through six ten respectively fasten the fittings
101. ot use an excessively damp cloth during cleaning gt Drain all solvent lines before opening any connections in the flow path 116 1290 Infinity TCC User Manual Maintenance 8 Changing Column Identification Tags The column compartment is equipped with a column identification system that stores column specific information Two identification tag readers are incorporated in the heat exchanger assemblies Tag readers Column clip c Figure 25 Column ldentification System When If column is used on the opposite heat exchanger or a tag is added to a new column Parts required p n Description 1 5062 8588 Column identification tag 1x re order 3 pk 1 The identification tag can be easily removed from the column 2 The tag needs to be placed differently depending on whether the column is installed at the left or right heat exchanger see Figure 26 on page 118 and Figure 27 on page 118 The Agilent logo should always be at front 1290 Infinity TCC User Manual 117 8 Maintenance When correctly placed on the heat exchanger the distance between tag and tag reader is 1 2 mm This is the optimum distance for proper function Column identification tag Figure 26 Column Identification Tag for Left Heat Exchanger Column identification tag Figure 27 Column Identification Tag for Right Heat Exchanger 3 For columns with small diameter a cable tie wrap should be used to fix the column identification tag to the column
102. oubleshooting and Diagnostics Agilent Lab Advisor Software 86 The Agilent Lab Advisor software is a standalone product that can be used with or without data system Agilent Lab Advisor software helps to manage the lab for high quality chromatographic results and can monitor in real time a single Agilent LC or all the Agilent GCs and LCs configured on the lab intranet Agilent Lab Advisor software provides diagnostic capabilities for all Agilent 1200 Infinity Series modules This includes diagnostic capabilities calibration procedures and maintenance routines for all the maintenance routines The Agilent Lab Advisor software also allows users to monitor the status of their LC instruments The Early Maintenance Feedback EMF feature helps to carry out preventive maintenance In addition users can generate a status report for each individual LC instrument The tests and diagnostic features as provided by the Agilent Lab Advisor software may differ from the descriptions in this manual For details refer to the Agilent Lab Advisor software help files The Instrument Utilities is a basic version of the Lab Advisor with limited functionality required for installation use and maintenance No advanced repair troubleshooting and monitoring functionality is included 1290 Infinity TCC User Manual 1290 Infinity TCC User Manual 70 Error Information e e e e What Are Error Messages 88 General Error Messages 89 Timeout 89 Shutdown
103. processor monitors the temperature change and checks if the temperature profile is changing in the correct direction If the temperature is not changing as expected the error message is generated Probable cause Suggested actions 1 Defective heater assembly Please contact your Agilent service representative 2 Defective TCC board Please contact your Agilent service representative Column Temperature Error ID 2836 The temperature of the column heat exchanger has exceeded the maximum limit Column Temperature 0 left heater Column Temperature 2 right heater For safety reasons the maximum column heat exchanger temperature is 105 C If an electronic failure occurs which causes the heater to heat continually the current is switched off when the temperature exceeds 105 C and the error message is generated Probable cause Suggested actions 1 Defective heater assembly Please contact your Agilent service representative 2 Defective TCC board Please contact your Agilent service representative 1290 Infinity TCC User Manual Error Information 6 Heatsink Temperature Error ID 2837 The temperature of the Peltier heatsink has exceeded the maximum limit Heatsink Temperature 0 left heater Heatsink Temperature 2 right heater The maximum temperature of the Peltier heatsink is 70 C If an electronic failure occurs which causes the heatsink to reach 70 C the current is switched OFF and the error message is generated
104. r Manual Appendix 12 The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC Abstract The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC adopted by EU Commission on 13 February 2003 is introducing producer responsibility on all electric and electronic appliances starting with 13 August 2005 This product complies with the WEEE Directive 2002 96 EC marking requirements The affixed label indicates that you must not discard this electrical electronic product in domestic household waste Product Category With reference to the equipment types in the WEEE Directive Annex I this product is classed as a Monitoring and Control Instrumentation product Do not dispose off in domestic household waste To return unwanted products contact your local Agilent office or see www agilent com for more information 1290 Infinity TCC User Manual 201 12 Appendix Lithium Batteries Information Lithium batteries may not be disposed off into the domestic waste Transportation of discharged Lithium batteries through carriers regulated by IATA ICAO ADR RID IMDG is not allowed Danger of explosion if battery is incorrectly replaced gt Discharged Lithium batteries shall be disposed off locally according to national waste disposal regulations for batteries gt Replace only with the same or equivalent type recommended by the equipment manufacturer Lithiumbatteri Eksplosionsfare v
105. r have a separate written agreement with warranty terms covering the material in this document that conflict with these terms the warranty terms in the sep arate agreement shall control Technology Licenses The hardware and or software described in this document are furnished under a license and may be used or copied only in accor dance with the terms of such license Restricted Rights Legend If software is for use in the performance of a U S Government prime contract or subcon tract Software is delivered and licensed as Commercial computer software as defined in DFAR 252 227 7014 June 1995 or as a commercial item as defined in FAR 2 101 a or as Restricted computer soft ware as defined in FAR 52 227 19 June 1987 or any equivalent agency regulation or contract clause Use duplication or dis closure of Software is subject to Agilent Technologies standard commercial license terms and non DOD Departments and Agencies of the U S Government will receive no greater than Restricted Rights as defined in FAR 52 227 19 c 1 2 June 1987 U S Government users will receive no greater than Limited Rights as defined in FAR 52 227 14 June 1987 or DFAR 252 227 7015 b 2 November 1995 as applicable in any technical data Safety Notices CAUTION A CAUTION notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly per formed or adhered to
106. ramic sapphire PTFE PEEK fused silica PEEK fused silica PTFE 1290 Infinity TCC User Manual 21 1 Introduction to the Column Compartment Table 2 Bio inert materials used in Agilent 1260 Infinity Systems Module Materials Bio inert heat exchanger G5616 60050 PEEK steel cladded for Agilent 1290 Infinity Thermostatted Column Compartment G1316C Bio inert Valve heads G4235A G5631A G5639A PEEK ceramic Alz03 based Bio inert Connection capillaries Upstream of sample introduction Titanium Downstream of sample introduction e Agilent uses stainless steel cladded PEEK capillaries which keep the flow path free of steel and provide pressure stability to more than 600 bar To ensure optimum bio compatibility of your Agilent 1260 Infinity Bio inert LC system do not include non inert standard modules or parts to the flow path Do not use any parts that are not labeled as Agilent Bio inert For solvent compatibility of these materials see Solvent information for parts of the 1260 Infinity Bio inert LC system on page 36 22 1290 Infinity TCC User Manual 1290 Infinity TCC User Manual 2 Site Requirements and Specifications Site Requirements 24 Physical Specifications 27 Performance Specifications 28 Valve Specifications 29 Extended Specifications onG1316C 34 Solvent Information 35 This chapter provides information on environmental requirements physical and performance specifications
107. re available from the Agilent web http www chem agilent com _layouts agilent downloadFirmware aspx whid 6976 1 1290 Infinity TCC User Manual Hardware Information 11 Electrical Connections The CAN bus is a serial bus with high speed data transfer The two connectors for the CAN bus are used for internal module data transfer and synchronization e One analog output provides signals for integrators or data handling systems The REMOTE connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features such as start stop common shut down prepare and so on With the appropriate software the RS 232C connector may be used to control the module from a computer through a RS 232C connection This connector is activated and can be configured with the configuration switch The power input socket accepts a line voltage of 100 240 VAC 10 with a line frequency of 50 or 60 Hz Maximum power consumption varies by module There is no voltage selector on your module because the power supply has wide ranging capability There are no externally accessible fuses because automatic electronic fuses are implemented in the power supply Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations 1290 Infinity TCC User Manual 181 11 Hardware Information 182 Serial Number Information
108. request Low 1290 Infinity TCC User Manual BCD Cables Identifying Cables 10 One end of these cables provides a 15 pin BCD connector to be connected to the Agilent modules The other end depends on the instrument to be connected to Agilent Module to General Purpose p n G1351 81600 Wire Color Pin Agilent Signal Name BCD Digit module Green 1 BCD 5 20 pea Violet 2 BCD 7 80 AA Blue 3 BCD 6 40 a ee Yellow 4 BCD 4 10 Black 5 BCD 0 1 Ras Orange 6 BCD 3 8 Red 7 BCD 2 4 Brown 8 BCD 1 2 Gray 9 Digital ground Gray Gray pink 10 BCD 11 800 Red blue 11 BCD 10 400 White green 12 BCD 9 200 Brown green 13 BCD 8 100 notconnected 14 notconnected 15 5V Low 1290 Infinity TCC User Manual 171 10 Identifying Cables Agilent Module to 3396 Integrators p n 03396 60560 Pin 3396 Pin Agilent Signal Name BCD Digit module an 1 1 BCD 5 20 2 2 BCD7 80 Se 15 oO Z 3 3 BCD 6 40 oO So 4 4 BCD 4 10 s je 2 5 5 BCDO 1 fie e9 6 6 BCD 3 8 7 7 BCD 2 4 8 8 BCD 1 2 9 9 Digital ground NC 15 5V Low 172 1290 Infinity TCC User Manual CAN Cable Identifying Cables 10 Both ends of this cable provide a modular plug to be connected to Agilent modules CAN or LAN connectors CAN Cables p n 5181 1516 5181 1519 LAN Cables p n 5023 0203 5023 0202 1290 Infinity TCC User Manual Description CAN cable Agilent module to module 0 5 m CAN cable
109. rned off NOTE The module was shipped with default configuration settings For changing these settings refer to section Setting the 8 bit configuration switch 1290 Infinity TCC User Manual 49 3 Installing the Module Installing Valve Heads 50 Installing the Valve Heads The valve drives are factory installed in the 1290 Infinity Thermostatted Column Compartment in the 1290 Infinity Flexible Cube and in the 1290 Infinity Universal Valve Drive The valve heads are interchangeable and can be easily mounted At the first installation the transportation lock TCC only and the dummy valve have to be removed see Removing the Transportation Lock and the Valve Dummy on page 51 The valve heads can be installed by mounting the valve heads onto the valve drives and fastening the nut manually do not use any tools Be sure that the guide pin snaps into the groove of the valve drive thread The valves are mounted on pull out rails to allow easy installation of capillaries Push the valve gently into its housing until it snaps into the inner position push it again and it slides out If all capillaries are installed push the valve back into its housing see Installing the Valve Head and Connecting Capillaries on page 52 1290 Infinity TCC User Manual Installing the Module Removing the Transportation Lock and the Valve Dummy The following procedure demonstrates the necessary steps for installing the valve he
110. rror conditions 7 Test Functions and Calibrations This chapter describes the TCC s built in test functions 1290 Infinity TCC User Manual In This Guide 8 Maintenance This chapter describes the maintenance tasks for the TCC If the instrument needs repair please contact your Agilent service representative 9 Parts and Materials for Maintenance This chapter provides information on parts for maintenance 10 Identifying Cables This chapter summarizes information on all cables 11 Hardware Information This chapter describes the TCC in more detail on hardware and electronics 12 Appendix This chapter provides addition information on safety legal and web 4 1290 Infinity TCC User Manual Contents Contents 1 Introduction to the Column Compartment 9 Overview of the Module 10 System Overview 11 Column ldentification System 13 Column Switching Valve Optional 15 Bio inert Materials 21 2 Site Requirements and Specifications 23 Site Requirements 24 Physical Specifications 27 Performance Specifications 28 Valve Specifications 29 Extended Specifications on G1316C 34 Solvent Information 35 3 Installing the Module 39 Unpacking the module 40 Optimizing the Stack Configuration 41 Installing the Column Compartment 46 Installing Valve Heads 50 Installation of Heater Devices 56 Flow Connections of the Column Compartment 60 Installing Capillaries 67 Placing Columns 70 4 How to optimize the Colu
111. se a Screw M4 2680 0128 for fixing the lock plate to the Transportation valve head G1316 40002 in the central position of the plate Put the spring washer not shown between the screw and the lock plate 142 1290 Infinity TCC User Manual Maintenance 8 Replacing the Module Firmware When Tools required OR OR Parts required Preparations The installation of newer firmware might be necessary if a newer version solves problems of older versions or to keep all systems on the same validated revision The installation of older firmware might be necessary to keep all systems on the same validated revision or if anew module with newer firmware is added to a system or if third party control software requires a special version Description LAN RS 232 Firmware Update Tool Agilent Lab Advisor software Instant Pilot G4208A only if supported by module Description 1 Firmware tools and documentation from Agilent web site Read update documentation provided with the Firmware Update Tool To upgrade downgrade the module s firmware carry out the following steps 1 Download the required module firmware the latest LAN RS 232 FW Update Tool and the documentation from the Agilent web http www chem agilent com _layouts agilent downloadFirmware aspx whid 69761 2 For loading the firmware into the module follow the instructions in the documentation Table 21 Module Specific Information G1316C
112. ss Steel Port size Accepts M4 male threaded fittings Flow passage diameters 0 25 mm 0 010 in Port to Port Volume 0 51 uL pre column volume 1 28 uL post column volume Maximum pressure 600 bar Table 13 G4234B 6 Column Selection Valve 1200bar Type Specification Liquid contacts PEEK Stainless Steel Port size Accepts M4 male threaded fittings Flow passage diameters 0 25 mm 0 010 in Port to Port Volume 0 51 uL pre column volume 1 28 uL post column volume Maximum pressure 1200 bar 1290 Infinity TCC User Manual 31 32 2 Site Requirements and Specifications Table 14 G4235A Solvent Selection valve head bio inert 200 bar Type Specification Liquid contacts PEEK Port size Accepts 10 32 male threaded fittings Flow passage diameters 1 00 mm 0 040 in Port to Port Volume 16 4 uL Maximum pressure 200 bar Table 15 G4236A 2D LC Valve Kit 1200 bar Type Specification Liquid contacts Vespel Port size Accepts 10 32 male threaded fittings Flow passage diameters 0 20 mm 0 008 in Port to Port Volume 0 27 uL via inner groove 0 24 uL via outer groove Maximum pressure 1200 bar Table 16 G5631A Bio inert 2pos 6port Valve Head Type Specification Liquid contacts PEEK Ceramic Port size Accepts 10 32 male threaded fittings Flow passage diameters 0 38 mm 0 015 in Stator 0 30 mm 0 012 in Rotor Seal Port to Port Volume 1 71 uL Maximum pressure 600 bar
113. stems The following kits are available for method development For more detailed information refer to Agilent 1200 Series Multi Method and Method Development System System Manual G4230 90001 p n 5067 4601 5067 1595 5067 1596 5067 1597 5190 1431 5190 1432 5190 1433 5190 1434 5190 1435 5190 1436 1290 Infinity TCC User Manual Description Solvent selection tubing kit 4 solvents Method development capillary kit low dispersion short column Method development capillary kit low dispersion long column Method development capillary kit general purpose RRHT Selectivity Method Development Kit 2 1 mm i d RRHT pH Method Development Kit 2 1 mm i d RRHT Selectivity Method Development Kit 4 6 mm i d RRHT pH Method Development Kit 4 6 mm i d Rapid Resolution Selectivity Method Development Kit Rapid Resolution pH Method Development Kit 157 9 Parts and Materials for Maintenance Capillary kit PN 5067 4646 The capillary kit PN 5067 4646 contains the following parts p n 5067 4647 5067 4648 5067 4649 2x 5067 4650 2x 5067 4651 2x 5067 4652 5067 4653 0890 1713 61316 80002 61316 80003 61316 89200 2x 61316 68706 2x 5042 9918 0100 1259 4x 158 Description Capillary ST 0 12 mm x 340 mm S SX Autosampler to Valve Capillary ST 0 17 mm x 700 mm S SX Pump to valve ACR only Capillary ST 0 12 mm x 90 mm S SX Valve to heat exchanger Capillary ST 0 12 mm x 150 mm SL SX short
114. t Heating Devices in the 1290 Infinity Thermostatted Column Compartment G1316C M For bio inert modules use bio inert parts only inert In this note the installation of the Bio Inert Solvent Heating Devices 9 uL internal volume 600 bar max pressure G5616 60050 into the Agilent 1290 Infinity TCC G1316C is described The additional solvent heating devices can be arranged in the G1316C in various locations depending on the application needs Some examples are shown in Figure 17 on page 58 Figure 17 Arrangements of the bio inert solvent heating devices in the 1290 Series TCC G1316C NOTE If the solvent heating devices are installed in the central position on the TCC s heat exchangers as shown in Figure 17 on page 58 the column identification system cannot be used The solvent heating devices are mounted directly onto the TCC s heat exchangers NOTE Don t forget to remove the protection foil from the heating transfer pad at the rear of the solvent heating device before installation 58 1290 Infinity TCC User Manual Installing the Module 3 Installation of Heater Devices Heat exchanger Solvent heating device SST cladded PEEK capillary bio inert Connection fittings bio inert Figure 18 Installing the solvent heating device CAUTION Strong force torque will damage SST cladded PEEK capillaries Be careful when installing stai
115. t the instrument at your site and initiate appropriate actions CAUTION The valve actuator contains sensitive optical parts which need to be protected from dust and other pollutions Pollution of these parts can impair the accurate selection of valve ports and therefore bias measurement results gt Always install a valve head for operation and storage For protecting the actuator a dummy valve head part of Transportation Lock Kit G1316 67001 can be used instead of a functional valve Do not touch parts inside the actuator 46 The tag reader reads the valve head properties from the valve head RFID tag during initialization of the module Valve properties will not be updated if the valve head is replaced while the module is on Selection of valve port positions can fail if the instrument does not know the properties of the installed valve Always switch off the instrument when replacing the valve head 1290 Infinity TCC User Manual Installing the Module 3 Installing the Column Compartment If the Thermostatted Column Compartment includes the valve drive option it is shipped with a transportation lock which needs to be removed during installation 1 Remove the 5 screws which hold the lock in position 2 Remove the dummy valve head by unscrewing the cap nut and removing it from the valve drive 3 Place the module on the bench in a horizontal position 1290 Infinity TCC User Manual 47 3 Installing th
116. tainless steel and free of other alloys containing metals such as iron nickel cobalt chromium molybdenum or copper which can interfere with biological samples The flow downstream of the sample introduction contains no metals whatsoever Table 2 Bio inert materials used in Agilent 1260 Infinity Systems Module Materials Agilent 1260 Infinity Bio inert Quaternary Pump G5611A Agilent 1260 Infinity Bio inert High Performance Autosampler G5667A Agilent 1260 Infinity Bio inert Manual Injector G5628A Agilent 1260 Infinity Bio inert Analytical Fraction Collector G5664A Bio inert Flow Cells Standard flow cell bio inert 10 mm 13 pL 120 bar 12 MPa for MWD DAD includes Capillary Kit Flow Cells BIO p n G5615 68755 G5615 60022 for Agilent 1260 Infinity Diode Array Detectors DAD G1315C D Max Light Cartridge Cell Bio inert 10 mm V s 1 0 pL G5615 60018 and Max Light Cartridge Cell Bio inert 60 mm V s 4 0 uL G5615 60017 for Agilent 1200 Infinity Series Diode Array Detectors DAD G4212A B Bio inert flow cell 8 uL 20 bar pH 1 12 includes Capillary Kit Flow Cells BIO p n G5615 68755 G5615 60005 for Agilent 1260 Infinity Fluorescence Detector FLD G1321B Titanium gold platinum iridium ceramic ruby PTFE PEEK Upstream of sample introduction Titanium gold PTFE PEEK ceramic Downstream of sample introduction PEEK ceramic PEEK ceramic PEEK ceramic PTFE PEEK ce
117. tandard HPLC pH 1 12 5 It can be corroded by acids below pH 2 3 In general following solvents may cause corrosion and should be avoided with stainless steel Solutions of alkali halides their respective acids for example lithium iodide potassium chloride and so on and aequous solutions of halogenes High concentrations of inorganic acids like nitric acid sulfuric acid and organic solvents especially at higher temperatures replace if your chromatography method allows by phosphoric acid or phosphate buffer which are less corrosive against stainless steel Halogenated solvents or mixtures which form radicals and or acids for example 2 CHCl Os gt 2 COCl 2 HCl This reaction in which stainless steel probably acts as a catalyst occurs quickly with dried chloroform if the drying process removes the stabilizing alcohol Chromatographic grade ethers which can contain peroxides for example THF dioxane di isopropylether such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides 1290 Infinity TCC User Manual 35 2 36 Site Requirements and Specifications e Solutions of organic acids acetic acid formic acid and so on in organic solvents For example a 1 solution of acetic acid in methanol will attack steel e Solutions containing strong complexing agents for example EDTA ethylene diamine tetra acetic acid Mixtures of carbon tetrachloride with 2 propanol or TH
118. tector is part of the system the LAN interface should be installed in the pump or autosampler RS 232C Serial The RS 232C connector is used to control the module from a computer through RS 232C connection using the appropriate software This connector can be configured with the configuration switch module at the rear of the module Refer to Communication Settings for RS 232C There is no configuration possible on main boards with on board LAN These are pre configured for 19200 baud e 8 data bit with no parity and one start bit and one stop bit are always used not selectable The RS 232C is designed as DCE data communication equipment with a 9 pin male SUB D type connector The pins are defined as 1290 Infinity TCC User Manual Hardware Information 11 Table 27 RS 232C Connection Table Pin Direction Function 1 In DCD 2 In RxD 3 Out TxD 4 Out DTR 5 Ground 6 In DSR 7 Out RTS 8 In CTS 9 In RI Instrument PC DCD 1 1 DCD RX 2 n 2 RX TX 3 X 3 TX DTR 4 4 DTR GND 5 5 GND DSR 6 6 DSR RTS 7 V 7 RTS CTS 8 M 8 CTS RI 9 9 RI DB9 DB9 DB9 DB9 Male Female Female Male Figure 45 RS 232 Cable Analog Signal Output The analog signal output can be distributed to a recording device For details refer to the description of the module s main board 1290 Infinity TCC User Manual 187 11 Hardware Information 188 APG Remote The APG Remote connector may be used in combination with ot
119. ter provides addition information on safety legal and web Ee Agilent Technologies 197 12 Appendix General Safety Information Safety Symbols Table 36 Safety Symbols Symbol Description The apparatus is marked with this symbol when the user should refer to the instruction manual in order to protect risk of harm to the operator and to protect the apparatus against damage Indicates dangerous voltages Indicates a protected ground terminal Indicates eye damage may result from directly viewing the light produced by the deuterium lamp used in this product The apparatus is marked with this symbol when hot surfaces are available A and the user should not touch it when heated up WARNING ahaa alerts you to situations that could cause physical injury or death gt Do not proceed beyond a warning until you have fully understood and met the indicated conditions CAUTION A CAUTION alerts you to situations that could cause loss of data or damage of equipment gt Do not proceed beyond a caution until you have fully understood and met the indicated conditions 198 1290 Infinity TCC User Manual Appendix 12 General Safety Information The following general safety precautions must be observed during all phases of operation service and repair of this instrument Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design manufac
120. the leak sensor is cooled by the solvent This changes the resistance of the leak sensor which is sensed by the leak sensor circuit on the main board Probable cause 1 Condensation Loose column fittings Broken capillary Aa Ww N Leaking column switching valve seal Leak Sensor Open Error ID 0083 Suggested actions Use a higher temperature setpoint Ensure all fittings are tight Exchange defective capillaries Exchange the valve seal The leak sensor in the module has failed open circuit The current through the leak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current falls outside the lower limit the error message is generated Probable cause 1 Leak sensor not connected to the main board 2 Defective leak sensor 3 Leak sensor incorrectly routed being pinched by a metal component 1290 Infinity TCC User Manual Suggested actions Please contact your Agilent service representative Please contact your Agilent service representative Please contact your Agilent service representative 91 92 Error Information Leak Sensor Short Error ID 0082 The leak sensor in the module has failed short circuit The current through the leak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change withi
121. the sensor falls below the lower limit the error message is generated Probable cause Suggested actions 1 Defective main board Please contact your Agilent service representative 1290 Infinity TCC User Manual 93 94 Error Information Left Fan Failed Error ID 2829 The left cooling fan in the column compartment has failed The hall sensor on the fan shaft is used by the TCC board to monitor the fan speed If the fan speed falls below 2 revolutions second for longer than 5 s the error message is generated Probable cause Suggested actions 1 Fan cable disconnected Please contact your Agilent service representative 2 Defective fan Please contact your Agilent service representative 3 Defective TCC board Please contact your Agilent service representative Right Fan Failed Error ID 2830 The right cooling fan in the column compartment has failed The hall sensor on the fan shaft is used by the TCC board to monitor the fan speed If the fan speed falls below 2 revolutions second for longer than 5 s the error message is generated Probable cause Suggested actions 1 Fan cable disconnected Please contact your Agilent service representative 2 Defective fan Please contact your Agilent service representative 3 Defective TCC board Please contact your Agilent service representative 1290 Infinity TCC User Manual Error Information 6 Open Cover Error ID The top foam has been removed The sensor on th
122. ture and intended use of the instrument Agilent Technologies assumes no liability for the customer s failure to comply with these requirements WARNING Ensure the proper usage of the equipment The protection provided by the equipment may be impaired gt The operator of this instrument is advised to use the equipment in a manner as specified in this manual Safety Standards This is a Safety Class I instrument provided with terminal for protective earthing and has been manufactured and tested according to international safety standards 1290 Infinity TCC User Manual 199 12 Appendix Operation 200 Before applying power comply with the installation section Additionally the following must be observed Do not remove instrument covers when operating Before the instrument is switched on all protective earth terminals extension cords auto transformers and devices connected to it must be connected to a protective earth via a ground socket Any interruption of the protective earth grounding will cause a potential shock hazard that could result in serious personal injury Whenever it is likely that the protection has been impaired the instrument must be made inoperative and be secured against any intended operation Make sure that only fuses with the required rated current and of the specified type normal blow time delay and so on are used for replacement The use of repaired fuses and the short circuiting of fus
123. ument up again the instrument firmware stays in the resident mode It is not operable as a module It only uses basic functions of the operating system for example for communication In this mode the main firmware can be loaded using update utilities Table 34 Boot Resident Settings without on board LAN Mode Select sw1 sw2 SW3 sw4 SW5 SW6 SW7 sws TEST BOOT 1 1 0 0 1 0 0 0 Forced Cold Start A forced cold start can be used to bring the module into a defined mode with default parameter settings CAUTION Loss of data Forced cold start erases all methods and data stored in the non volatile memory Exceptions are calibration settings diagnosis and repair log books which will not be erased gt Save your methods and data before executing a forced cold start If you use the following switch settings and power the instrument up again a forced cold start has been completed Table 35 Forced Cold Start Settings without on board LAN Mode Select sw1 sw2 SW3 sw4 SW5 SW6 SW7 SW8 TEST BOOT 1 1 0 0 1 0 0 1 1290 Infinity TCC User Manual 193 11 Hardware Information Early Maintenance Feedback 194 Maintenance requires the exchange of components which are subject to wear or stress Ideally the frequency at which components are exchanged should be based on the intensity of usage of the module and the analytical conditions and not on a predefined time interval The early maintenance feedback EMF feature mo
124. uration switch settings depend on configured mode All modules without on board LAN default should be ALL DIPS DOWN best settings Bootp mode for LAN and 19200 baud 8 data bit 1 stop bit with no parity for RS 232 DIP 1 DOWN and DIP 2 UP allows special RS 232 settings e for boot test modes DIPS 1 2 must be UP plus required mode For normal operation use the default best settings Switch settings provide configuration parameters for serial communication protocol and instrument specific initialization procedures With the introduction of the Agilent 1260 Infinity all GPIB interfaces have been removed The preferred communication is LAN 1290 Infinity TCC User Manual Hardware Information 11 The following tables represent the configuration switch settings for the modules without on board LAN only Table 29 8 bit Configuration Switch without on board LAN Mode Select 1 2 3 4 5 6 7 8 RS 232C 0 1 Baudrate Data Parity Bits Reserved 1 0 Reserved TEST BOOT 1 1 RSVD SYS RSVD RSVD FC The LAN settings are done on the LAN Interface Card G1369B C Refer to the documentation provided with the card Communication Settings for RS 232C The communication protocol used in the column compartment supports only hardware handshake CTS RTR Switches 1 in down and 2 in up position define that the RS 232C parameters will be changed Once the change has been completed the column instrument must be powered up again
125. use Suggested actions 1 Not ready condition in one of the Ensure the instrument showing the not ready instruments connected to the remote line condition is installed correctly and is set up correctly for analysis 2 Defective remote cable Exchange the remote cable 3 Defective components in the instrument Check the instrument for defects refer to the showing the not ready condition instrument s documentation Lost CAN Partner Error ID 0071 During an analysis the internal synchronization or communication between one or more of the modules in the system has failed The system processors continually monitor the system configuration If one or more of the modules is no longer recognized as being connected to the system the error message is generated Probable cause Suggested actions 1 CAN cable disconnected Ensure all the CAN cables are connected correctly e Ensure all CAN cables are installed correctly 2 Defective CAN cable Exchange the CAN cable 3 Defective main board in another module Switch off the system Restart the system and determine which module or modules are not recognized by the system 1290 Infinity TCC User Manual Leak Error ID 0064 A leak was detected in the module Error Information 6 The signals from the two temperature sensors leak sensor and board mounted temperature compensation sensor are used by the leak algorithm to determine whether a leak is present When a leak occurs
126. valid information The RFID can be damaged if the instrument is power cycled during a write access of the tag Probable cause Suggested actions 1 RFID tag contains invalid information Replace valve head see Valve Options Overview on page 153 100 1290 Infinity TCC User Manual Door sensor HW failure Error ID 2873 Error Information 6 This error is generated if a front door is detected while the front door sensor has been disabled during the self test Probable cause 1 Defect in cable connection of door sensor to main board 2 Door sensor defective Valve RFID access failure Error ID 2874 Suggested actions Please contact your Agilent service representative Please contact your Agilent service representative The valve tag reader fails reading or writing the RFID tag of the valve head Probable cause 1 Defect in cable connection of valve tag reader to main board 2 Valve head not installed correctly 3 RFID tag defective 4 Valve tag reader is damaged 1290 Infinity TCC User Manual Suggested actions Please contact your Agilent service representative Review installation of valve head see Installing the Valve Head and Connecting Capillaries on page 52 Replace valve head see Replacing Valve Heads on page 136 Please contact your Agilent service representative 101 6 Error Information TCC Error Messages 102 1290 Infinity TCC User Manual 1290 Infinity T
127. y volume For even lower band broadening the heat exchanger can be by passed and the column is placed well between the heat exchanger fins Keep the left and right heat exchanger temperature the same unless you do specific applications Assure that the front cover is always closed 1290 Infinity TCC User Manual How to optimize the Column Compartment 4 Using Additional Heater Devices The optimization installation interconnection and specific settings when using additional heating devices are described in the Agilent 1290 Infinity LC System System Manual and Quick Reference G4220 90301 1290 Infinity TCC User Manual 75 4 How to optimize the Column Compartment Delay Volume and Extra Column Volume The delay volume is defined as the system volume between the point of mixing in the pump and the top of the column The extra column volume is defined as the volume between the injection point and the detection point excluding the volume in the column Delay Volume In gradient separations this volume causes a delay between the mixture changing in the pump and that change reaching the column The delay depends on the flow rate and the delay volume of the system In effect this means that in every HPLC system there is an additional isocratic segment in the gradient profile at the start of every run Usually the gradient profile is reported in terms of the mixture settings at the pump and the delay volume is not quoted even t

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