- User Manual
Contents
1. Kai 211 bis avenue JF Kennedy BP 1140 03100 Montlucon FRANCE Wace ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com User Manual interfine interchim fr Removal of protective panels may only be performed by an authorized person When it is necessary to use a non original power cord plug make sure the replacement cord adheres to the color coding and polarity described in the manual and local building safety codes Replace blown fuses with fuses of the size and rating stipulated in the manual Replace faulty or frayed power cords immediately with the same type and rating Make sure that voltage sources and line voltage match the value for which the instrument is wired To ensure your own safety thus of your co workers and safe operation of the equipment observe the following instructions v Use a 3 wire mains socket with ground connection grounding is necessary to ensure operator safety and proper operation Take care of inflammable solvents Store the system after having passed a clean solvent in the hydraulic circuit when not in use Do not leave buffer in the system it may cause blockages and damage the seals on start up Before moving the instrument the external connections have to be disconnected the hydraulic circuit rinsed and the inlets outlets corked The instrument must be carefully carried in its original box 6 gt Maintenance 6 1 Introduction This chapter describes actions that should be perfo2. DET of the unit control Screw the other end round female connector in the back panel of the detector Link the unit control label COM2 DETECTOR and the detector module using the Sub D9 female female cable supplied Do not forget to set the connectors by fastening the two fixing screws Pump Back panel of the pump Serial port gt COM1 Switch 0 1 8 pin connector Axial fan PR Fuses drawer Power plug Take the power cable for main voltage supply and connect it at the same time in the plugs of the unit control label PUMP and in the sockets of the pump module Link the unit control label COM1 PUMP and the pump module using the Sub D9 male female cable supplied Do not forget to set the connectors by fastening the two fixing screws Collector Back panel of the fraction collector External controls Switch 0 1 Serial port gt COM3 Analogic Fuses drawer inputs for second detector acquisition Power plug Take the power cable for main voltage supply and connect it at the same time in the plugs of the unit control label COLL and in the sockets of the pump module Link the unit control label COM3 COLLECTOR and the collector module using the Sub D9 male female cable supplied Do not forget to set the connectors by fastening the two fixing screws Plug the AC power cable supplied in the general switch located to the left side of the system and then
3. E H RE SW E j A E Ai E 5 LE me Le a i a Es w EME Pl F w T a Once the user interface opened several graphic elements allow to see in real time the state of the instruments and the evolution of the working parameters The application has been developped with the aim of being convivial and intuitive Refer to the Instruction Manual supplied for more explanations on the software use Use the stylus on the tactile screen to create methods in the software and control the system D D a 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE TAC re 33 470 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com 33 4 70 03 73 01 4 2 Make a liquid injection The liquid injection is done directly through the chromatography column by using a syringe Injection Mode Equilibration Wait for Inj In the panel Settings of the method edited select Equilibration in the section Injection Mode According to the type of column chosen a time and a flow rate are written by default they depend on its parameters but you can modify them if needed After having launched the method RUN the equilibration phase starts That allows to condition the column with the solvents used At the end of the time or at a click on START the pump stops and the following dialog box appears on the screen P
4. Ensure that the arrow is directed downwards during the cleaning Else damage to the surface quality or the ball and the seat will occur 7 Replace the check valves Whether it is for the inlet 20mm or the outlet 10mm make sure the arrow is always directed upwards Inlet Suction Outlet Delivery Check valve housing Check valve S 10mm Check valve 20mm es RRRRREE 8 Hard tighten the valve housing in the head until hearing a little crunch which means that the new check valve is enough tight and the imperviousness properly done This noise corresponds to the collapsing of the top and bottom membranes 9 Connect the Suction and Delivery tubing and fittings D D a 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE TAC re 33 470 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com 33 4 70 03 73 01 6 5 Replacing the detector deuterium lamp 1 Purge the system with a suitable solvent with then alcohol and with air 2 Switch off the system and disconnect it from the electrical supply It will be reconnected only when the panel will be installed back in its place 3 Let the lamp cools down fifteen minutes after switching it off 4 Remove tubing and connections from the detector module and remove it from the whole system you must first remove the pump module 5 Position the detector module on its side in order to view the bottom and unscrew the three M3 cylindrical head sc
5. Installation procedure 6 Quad system Module for 4 additional columns installation Inlet tubing with stainless steel fitting set 1 8 ID 1 6mm 1 50m Outlet tubing 1 8 ID 1 6mm 1 50m PEEK 28 long nut for 1 8 tubing x 3 ETFE ferrule for 1 8 tubing x 3 CD for TRP converter drivers installation External control cable USB cable Power cable model according to the destination country Hardware Installation procedure 7 2 Acquisition channel Module USB 1408FS USB cable USB key for drivers installation Installation procedure 8 FLASH ELSD FLASH ELSD 1V Output cable Power cable Splitter box with micro needle option ETFE Tubing 1 16 ID 0 25mm Splitter Outlet ELSD Inlet length 30cm fitting 10 32 Male P N 780771 connected to Splitter box ETFE Tubing 1 8 ID 1 6mm Splitter Outlet UV Inlet length 72cm Adapter 4 28F 10 32 Male P N 737664 spring 20 cm P N PFS800 connected to Splitter box ETFE Tubing 1 8 ID 1 6mm Column Outlet Splitter Inlet length 72cm Adapter 1 4 28F to 10 32 Male P N 737664 connected to Splitter box Manometer P N FJ6720 option 2 red or white tubing to connect the gas 6mm OD 2m 1 m and fitting 1 PEEK fitting 10 32 Male fitting Inlet ELSD P N 780771 Adapter 1 4 28 Female 1 4 28 Female Nylon P N 187210 only for
6. alternating from one to the other 6 Carefully remove the pump head The piston and the seal are now visible D 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE MCE ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 www interchim com 33 4 70 03 73 01 6 3 Replacing a seal There are in all four seals one of every head and one of every cleaning disc You can exchange them by removing the pump heads and cleaning disc as explained previously 1 Remove the seal using a small screwdriver Be sure to avoid scoring any part of the pump head and the cleaning disc as a scratch will prevent proper sealing If the seat is scored it is necessary to replace the head or the cleaning disc and these are not under warranty Head back views Cleaning disc back views Seal seat 2 Thoroughly clean the seal seat with alcohol to remove possible seal particles and plunge the new seal in propanol 2 for lubrication 3 Place the new seal into the pump head or the cleaning disc 4 Note that the seal is well placed in the pump head or in the cleaning disc 5 Finally replace the cleaning disc and the head on the piston and put its two fixing screws with care alternating from one to the other 6 Connect the Suction and Delivery tubing and fittings Note The life time of the seals is dependent on the flow rate pressure type of liquids used and temperature but mostly on the cleanliness of the mob
7. FRANCE Wace ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com interfine interchim fr User Manual Set up the dry load and pre column To inject solid sample make dry loading injection or use the pre column you have the possibility to use a column of which the maximum dimensions are 27cm in height and 70mm for external diameter Install it as follows 1 Unscrew lightly the knurled knob of the holder in order to allow its vertical movement sB Move up the large black POM holder on the vertical guidance rail and place the bottom fitting of the dry load or pre column on the Adapter ETFE or Stainless steel Luer lock female to 14 28 male located on the stainless steel bulkhead union 90 A Move down the large black POM holder and lock again the blazzack ring of the fitting Adapter PEEK Luer lock male to 4 28 male to the top fitting of the dry load 2 Unlock the black ring of the fitting Adapter PEEK Luer lock male to 4 28 male of the pre column 3 4 Prepare the fractions collection According to the type of collection required small or big volumes short or long fractionation etc the equipments to install are different The standard rack set 29 5 x 200mm P N PF4440 is supplied by default CAUTION Before any action take a diagonal plier and carefully cut the cable tie which holds the arm during the transfer Dispense arm Cable Tie Place first the tubes in the
8. ME SL M Module ACTOR icra es eed ence a sede E cau E areas e E cua segsaeeee ade E a dase P N and description of defective spare ln e Any further action ee EI EE RI NAMO enoras aaraa ES Signature gt Service report lt O Installation O End of warranty visit O Warranty O Repair O Maintenance O Out of warranty CSC OS le ME NE E P Ville lei 1 ICS CO E gt Service report lt O Installation O End of warranty visit O Warranty O Repair O Maintenance O Out of warranty Systemi le e NEE Sh ME Ville AC E NR EE EE EEEE eA E E E TE E E P N and description of defective spare e Any further action required touiciicevcazsoxsencedesxcves lt estascsescubeshteycencexueetadssasbes vectutuxssateeentdanebesancesvseanternectsaxtevendnersieenedebaredy RE NOR en Signature gt Service report lt O Installation O End of warranty visit O Warranty O Repair O Maintenance O Out of warranty e gl e e ME SL M Module ACTOR icra es eed ence a sede E cau E areas e E cua segsaeeee ade E a dase P N and description of defective spare ln e Any further action ee EI EE RI NAMO enoras aaraa ES Signature gt Service report lt O Installation O End of warranty visit O Warranty O Repair O Maintenance O Out of warranty CSC OS le ME NE E P Ville lei 1 ICS CO E gt Service report lt O Installation O End of warranty visit O Warranty O Repair O Ma
9. in normal operating conditions and in accordance with the instructions The device must have been used with the consumables recommended by Interchim v Exhaustive list of original parts covered by the guarantee part 6 2 Il Guarantee exclusions The guarantee will not cover devices installed by the customer or by a service provider not approved by Interchim The guarantee will not cover equipment used in a manner which is non compliant with the provisions in the instructions The guarantee will not cover equipment subject to interventions repairs or modifications by personnel without Interchim approval The guarantee will not cover parts modified or changed by the customer or the service provider without Interchim s approval parts damaged by this modification parts not recommended by Interchim parts subject to wear and consumables The guarantee will not cover any parts not listed in part 6 2 v The guarantee will not cover electrical and or electronic and or IT incidents caused by external factors The guarantee will not cover damage caused to the software or hardware due to contamination by an IT virus The guarantee will not cover damage or failures caused by impact and or bad weather The guarantee will be cancelled in case of damage caused by abnormal mechanical forces applied to the device and exceeding the limits defined in the user guide v The guarantee will be cancelled in case of corrosion to the device due to solvent le
10. lock male to 28 Male of the pre column holder ee Move down the large black POM holder and lock again the black ring of the fitting adapter PEEK luer lock male to 1 4 28 male to the top fitting of the dry load After having launched the method RUN the equilibration phase starts That allows to condition the column with the solvents used At the end of the time or at a click on START the pump stops and the following dialog box appears on the screen PEs Move up the large black POM holder on the vertical guidance rail and place the bottom fitting of the dry load or pre column on the stainless steel luer lock female to 1 4 28 male on the stainless steel bulkhead union 90 A Answer Yes to the question of the software The method starts T l 33 4 70 03 88 55 Fax 33 4 70 03 82 60 www interchim com User Manual PUG LASH SOC 33 4 70 03 73 01 5 gt Safety regulations This equipment is built according to the E U security standards However risks and dangers could remain if the system is used in a different way from which it is intended or if it is used by untrained personnel The PUFUE LASH 5s OO should only be used by laboratory trained or experienced persons The user should inform the distributor in case of problems and safety related matters that occur during the use of the instrument Alerts you to potential hazardous situations that could W
11. page A 30 10 gt Specifications page A 36 gt Appendix Rack sets available page A 37 gt Appendix 2 Service report page A 38 Kai 211 bis avenue JF Kennedy BP 1140 03100 Montlucon FRANCE Wace ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com interfine interchim fr 1 gt General description The PUFF LASH ACC system is a modern instrument designed for Flash chromatography which allows to withdraw mobile phases from four containers of solvents form a gradient inject liquid or solid samples and provide the solution to a chromatography column A detector measures the absorbance and gives a signal called chromatogram which is visualized with the built in computer and the control software INTERCHIM Software installed Then fractions can be collected with the fraction collector The characteristics of a PURI LASH ACC are v Flow rate Up to 800mL min v Pressure Max 10 bar 145psi v Gradient Binary or Quaternary v Column size From F4 to F1600 format according to column trademark UV Detection Spectrophotometer dual wavelength DAD technology range 200 600nm Collection Several types of racks available The complete system consists of following modules The 2 head pump As mentioned above the pump can deliver mobile phase at flow rates up to eight hundred millilitres per minute with low residual pulsation It is a high performance double piston pump reciproca
12. plug in additional devices like second detector or options The maximum length of all external cables connected to the system is 2m LAN plug Local Area Network Serial communication de Others external port ze connectors Optional Level sensors plugs Kai 211 bis avenue JF Kennedy BP 1140 03100 Montlucon FRANCE Wace ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com User Manual interfine interchim fr External controls Analogic signal inputs 2 gt Packing list Standard system PYRI LASH 2 5 Item PURIFL SH ACC system composed of Pump module Column holder module Pre column holder Detector module Fraction collector module Computer amp power supply module v Tubing with fittings ETFE Suction OD 3 16 1 50m ID 3 2mm x4 or x2 depends to the gradient valve Gradient valve outlet to Tee Pump inlet OD 3 16 28cm ID 3 2mm present on the system Pump outlet to Pre column holder inlet OD 1 8 60cm ID 2 4mm Pre column holder outlet to column holder inlet OD 1 8 90cm ID 2 4mm Column holder outlet to detector inlet OD 1 8 70cm ID 2 4mm Detector outlet to fraction collector inlet OD 1 8 30cm ID 2 4mm Waste outlet OD 1 8 1 50m ID 2 4mm Pressure test OQ OD 1 16 50cm 1 16 ID 0 5mm v Rack set DT8250 for 29 5mm diameter tubes 4 racks v So
13. the PURI LASH SOO Installation procedure 9 Funnel rack set 1 unit or funnel rack set 4 units 10 Safety solvent caps kit tubing 3 16 11 Safety waste cap Container 20L Funnel Filter 12 Drainage System of the tray collector 13 Injection pump electric switching valve 200mL mm 30 bar 14 CarouXel Power cable RS 232 cable Installation procedure 15 Autosampler Power cable RS 232 cable User s manuel Installation procedure 16 Mass spectrometer interface 17 2 fraction collector coupled Fraction collector General power cable Serial communication cable Sub 09 male female Tubing with 2 fitings and 2 ferrules I 3m 1 8 ID 1 6mm 18 Ballasting kit for 3 16 and 1 8 tubing 19 Racks set 20 Stand Alone column holder for flash Kai 211 bis avenue JF Kennedy BP 1140 03100 Montlucon FRANCE CCE ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com User Manual interfine interchim fr 4 gt Operation a 4 1 Control the system When all the installations and connections are made switch on the PURI LASH 00 After few seconds and the initialisations of different instruments the control software Interchim Software automatically starts Note the good starting no message Communication Error is necessary for the correct working of the system el KT p m r PERCE CRA Pe a i ee A EEE ee SE SERA
14. 1 8 tubing for GV1690 GV1710 Adapter Luer Lock Male to 1 4 28 Male PEEK JO2630 Guidance part for large black POM holder FO4400 Stainless steel union for flash configuration FV6560 Large black POM holder grub screw 04960 Stainless Steel bulkhead union 90 PFS370 5 Adapter ETFE Luer Lock Female to 1 4 28 Male or PFS780 2 Adapter Stainless Steel Luer Lock Female to 1 4 28 Male GV1680 2 PEEK Ring Outlet column holder 102690 3 Screw pre column holder 109140 6 Kai 211 bis avenue JF Kennedy BP 1140 03100 Montlucon FRANCE Wace ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com interfine interchim fr User Manual Item Part Number N General Central Processing Unit Touch screen PFS520 USB hub PFS530 USB extension cord I 0 5cm PFS540 Stylus FK2400 AC power supply 230 24V 150W PFS550 UC axial fan V2 LV9380 Multi stage performance EMI filter PFS570 General ON OFF switch PFS590 General fuses x 2 3 15 A 220V PFTO10 General fuses x 2 6 3 A 110V PFT100 Serial communication cable male female PFS600 Serial communication cable female female PFS610 Distribution power cable male female for main voltage PFS620 Distribution power cable male female for 24V PFS630 Power cord FR PFS640 Power cord UK PFS650 Power cord US PFS660 10 gt Specifications v Model name PUR LASH ds OO system Serial number XXXX X XX v Flow rate range up to 800mL min v Flow rate performa
15. 73 01 Upkeep schedule By considering the use of clean solvents and all precautions already mentioned in the chapter 6 Maintenance here is a table which regroup the recommended actions in order to assuring a good working of the system Operation Pump Check the suitable tightening of fittings strongly hand tight and the proper state of tubing not bended Replace the tubing and associated fittings Clean and rinse the gradient valve with suitable solution Replace seals and backup rings Clean the check valves Replace the check valves Clean the piston rods Detector Check the efficiency of the deuterium lamp Replace the deuterium lamp Clean the flow cell General Verify the cleanliness of all liquid containers Check the suitable tightening of fittings strongly hand tight and the proper state of tubing not bended Clean the injection valves and all parts in contact with solvents or samples with suitable solutions Rinse abundantly using a mixing of water and alcohol Check the tangential fan working to maintain a good air recycling inside the different modules Frequency Weekly or when inaccuracy flow rates solvents leaks or air bubbles are observed Yearly or when damaged parts are observed As often as possible specially after having pumped aqueous solvents or strong buffers Yearly Quarterly Yearly or when abnormal pressure flow rate fluctuation or noisy basel
16. A E A INTERFINE CHEMICALS ANALYTICAL SCIENCES BIOCHROMATOGRAPHY BIOSCIENCE INNOVATION perps 200 User Manual ae Process Chromatography Large Scale Purification Flash columns up to F1600 format amp more PURE LASH SOO 800 ml min up to 10 bar INTERFINE CHEMICALS 211 bis avenue JF Kenned ea j nte re h m BP 1140 03100 Montlu on Hotline 33 4 70 03 73 01 interfine interchim fr Fax 33 4 70 03 82 60 PUGUSLASH 33 4 70 03 73 01 Summary 1 gt General description page A 3 2 gt Packing list page A 7 3 gt Installation page A 8 3 1 Assembling the system Install the tubing and prepare the containers Set up the column the pre column and the dry load Prepare the fractions collection Position the stylus Make the connections electrical and data transmission Options and accessories NOOB WP 4 gt Operation page A 17 A 1 Control the system 4 2 Make a liquid injection 4 3 Make a solid injection 5 gt Safety regulations page A 20 6 gt Maintenance page A 21 1 Introduction 2 Removing a pump head 3 Replacing a seal 4 Cleaning replacing a check valve 5 6 Replacing the detector deuterium lamp Cleaning the detector flow cell 7 gt Preventive measures Upkeep schedule page A 26 8 gt Warranty page A 27 8 1 Generalities 8 2 Limited warranty of consumable parts 9 gt List of spare parts Blow up views
17. ARN N G result in serious injury or damage of the system This system is designed for chromatographic purposes It must be operated using appropriate solvents and within specified ranges for pressure flows and temperatures as described in this manual If it is used in a manner not specified by the manufacturer the protection provided by the equipment may be impaired When handling potentially hazardous solvents and samples it is important to follow standard laboratory safety procedures Common chemicals used in the laboratory can be toxic corrosive flammable or a combination of these properties A list of all safety aspects is outside the scope of this manual but the following points should be considered v Ensure adequate ventilation v Wear eye protection v Wear gloves when handling chemicals v Ensure all liquid containers are secure v No smocking or naked flames v Clean up all spillages W ARN I N G Eye damage could occur from particles or chemicals Use proper eye protection EYE HAZARD If a line ruptures or a valve opens accidentally under pressure a potentially hazardous spray of liquid might be generated by the pump Never open a solvent line or valve under pressure Stop the pump first and let the pressure drops to zero Wear protection glasses Hazardous voltages are present inside the WARN i N G instrument Disconnect from main power before removing screw attached panels SHOCK HAZARD
18. D D a 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE NCAA re 33 470 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com 33 4 70 03 73 01 8 2 Limited warranty of consumable parts Item Part Number Warranty period Fittings unions amp tubing TEFZEL ferrule with Stainless steel lock ring for 1 8 tubing PEEK 1 4 28 short nut for 1 8 tubing for GV1690 PEEK 1 4 28 long nut for 1 8 tubing for GV1690 PEEK 5 16 nut ETFE ferrule for 3 16 tubing Adapter Luer Lock Male to 1 4 28 Male PEEK Adapter ETFE Luer Lock Female to 1 4 28 Male Adapter Stainless Steel Luer Lock Female to 1 4 28 Male PEEK tee union for 3 16 tubing Stainless steel bulkhead union 90 Suction tube FEP 1 50m 3 16 ID 3 2mm 1 PEEK nut 1 ETFE ferrule x 2 Suction tube FEP 1 50m 3 16 ID 3 2mm 1 PEEK nut 1 ETFE ferrule x 4 Tube Gradient valve to Tee pump inlet FEP 28cm 3 16 ID 3 2mm 2 PEEK nuts 2 ETFE Ferrules Tube pump outlet to Pre column holder inlet ETFE 60cm 1 8 ID 2 4mm 2 PEEK nuts 2 TEFZEL Ferrules Tube Pre column holder outlet to Column holder inlet ETFE 90cm 1 8 ID 2 4mm 2 PEEK nuts 2 TEFZEL Ferrules Tube Column holder outlet to detector inlet ETFE 70cm 1 8 ID 2 4mm 2 PEEK nuts 2 TEFZEL Ferrules Tube Detector outlet to collector inlet ETFE 30cm 1 8 ID 2 4mm 2 PEEK nuts 2 TEFZEL F
19. ETFE Ferrules Tube pump outlet to Pre column holder inlet ETFE 60cm 1 8 ID 2 4mm 2 PEEK nuts LV9860 2 TEFZEL Ferrules Tube Pre column holder outlet to Column holder inlet ETFE 90cm 1 8 ID 2 4mm 2 PEEK nuts LV9870 2 TEFZEL Ferrules Tube Column holder outlet to detector inlet ETFE 70cm 1 8 ID 2 4mm 2 PEEK nuts LV9880 2 TEFZEL Ferrules Tube Detector outlet to collector inlet ETFE 30cm 1 8 ID 2 4mm LV9890 2 PEEK nuts 2 TEFZEL Ferrules Waste outlet tube ETFE 1 50m 1 8 ID 2 4mm LV9910 PEEK nut ETFE ferrule Tube Collection valve to collection needle ETFE 1m 1 8 ID 2 4mm 1 PEEK nut 1 TEFZEL Ferrule LV9900 spring pipe Spring for tubes 5cm PFS800 Spring for suction tubes 20cm PFTO30 Electronic boards Electronic board CPU2010 Electronic board Collector 102950 PFS500 System Flex for collector connections Kai 211 bis avenue JF Kennedy BP 1140 03100 Montlucon FRANCE Wace ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com PFS490 interfine interchim fr Item Pump Check valve 10mm Check valve housing 10mm Check valve 20mm Check valve housing 20mm Seal 14mm Head Piston 14 x 90 kit Cleaning disc Camshaft 2 Cams Compression spring Pump opto sensor cable Opto sensor disc Part Number PFS930 PFS010 LO2620 LV9580 LO2640 LV9590 LV9790 LV9800 LV9820 LV9810 PFS180 PF
20. Kennedy BP 1140 03100 Montlucon FRANCE CCI ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com User Manual interfine interchim fr 3 5 Position the stylus The stylus is supplied to control the system via computer touch screen A fixed holder allows to easily put it away when it is not used and its extensible cord linked to the holder gives the suitable length Stick the stylus holder just as you like in order to profit at best of its features i e to the left side of the system as showed on this picture 3 6 Make the connections electrical and data transmission The complete system is supplied with appropriate fuses and is ready to operate at the line voltage of the shipping destination The Computer module serves as the primary point for power distribution and data collection The ports on the modules are labelled to ensure proper connections Using the cables provided connect each module to the unit control Unit control Back panel of the unit control communication ports Power sockets Detector Back panel of the detector 24 V round connector gt E Axial fans Serial port gt COM2 D D 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE CTI ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 www interchim com PUG LASH xO 33 4 70 03 73 01 Take first the special cable for 24V supply and screw the round male connector in the suitable socket label
21. S190 User Manual N e OD NOORA D 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE MCE ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 www interchim com PURIFLASH P 33 4 70 03 73 01 Item Part Number N Pump suite Belt LV9970 Motor Black 101940 Motor driver Motor black 101950 Axial fan kit PFS320 AC Power supply 230 24V 60W PFS290 Pump Collector fuses 2 5 A 110V x 2 PFS990 Pump Collector fuses 1 6 A 220V x 2 PFS980 Kai 211 bis avenue JF Kennedy BP 1140 03100 Montlucon FRANCE Wace ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com interfine interchim fr User Manual Item Part Number N Pump suite Mixing chamber LV9610 Seal for mixing chamber LV9620 Frit 20um for mixing chamber x 2 PFS260 Pressure transducer seal LV9630 7 Pressure transducer cable LV9640 6 Leakage sensor PFS300 Detector Flow cell 0 3mm HO7270 PFS970 PFTO50 GV3320 LV7070 Deuterium lamp Axial Fan Power Board Detector fuse 500mA 250V D D a 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE TAC re 33 470 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com PUGS LASH SI Item Fraction collector Positioning guide for racks Fraction collector motor X axis cable Fraction collector motor Y axis cable X axis belt Y axis belt Pulleys 1 motor 1 receptor Collector opto sensor X axis Co
22. akage or samples The guarantee will be cancelled in case of corrosion to electronic components caused by highly corrosive gas The guarantee will not cover damage or failures caused by assembly dismantling modification or transport after initial installation The guarantee will be cancelled in case of failure or damage due to non compliance with the closing and shutdown procedure for the device The guarantee will be cancelled in case of failure or damage due to wrong installation The guarantee will be cancelled in case of failure or damage due to wrong AC power supply The guarantee will be cancelled in case of failure or damage due to mechanical force to the unit v The guarantee will be cancelled in case of failure or damage due to acts of nature The guarantee will be cancelled in case of failure or damage due to non compliance with safety procedures v The customer is liable for transport risks In case of damage during transport the beneficiary must issue all reserves to the transport firm before accepting the delivery of the device The guarantee will not cover damage caused in accidents external events contingencies or force majeure due to negligence or a lack of surveillance by the customer or due to non compliance with safety rules v The guarantee will not cover damage if the maintenance procedures recommended by the manufacturer are not complied with v Interchim will not guarantee the results of the use of the Sg LASH SOO SS
23. errules Waste outlet tube ETFE 1 50m 1 8 ID 2 4mm PEEK nut ETFE ferrule Tube Collection valve to collection needle ETFE 1m 1 8 ID 2 4mm 1 PEEK nut 1 TEFZEL Ferrule spring pipe gt Terms of use Normal tightening of the fittings Use of clean solvents and clean containers Follow up of the preventive maintenance actions gt Damages not covered GV1690 GV1700 GV1710 JO8520 JO2630 PFS780 GV1680 LV9960 PFS370 LV9830 LV9840 LV9850 LV9860 LV9870 LV9880 LV9890 LV9910 LV9900 Damaged thread or crushed internal part due to unsuitable tightening Blocked passage due to uncleanliness of installation or unsuitable mobile phase Kai 211 bis avenue JF Kennedy BP 1140 03100 Montlucon FRANCE CCI ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com interfine interchim fr Item Part Number Warranty period Valves Binary Gradient valve CPU2010 LV9550 Quaternary Gradient valve CPU2010 LV9570 Collection 3 ways valve HO7260 gt Terms of use Normal tightening of the fittings Use of clean solvents and clean containers Follow up of the preventive maintenance actions gt Damages not covered Damaged thread or crushed internal part due to unsuitable tightening Blocked passage due to uncleanliness of installation or unsuitable mobile phase Check valve 10mm PFS930 Check valve 20mm LO2620 Seal 14mm LO2640 Piston 14
24. g the 1 5m x 2 4mm tube attach the end with the fitting into the fraction collector waste port and hand tighten On the other end insert into an appropriate waste bottle 3 3 Set up the column the pre column and the dry load A large range of columns can be used on the column holder columns packed with normal or reverse phase with an external diameter up to 120mm The Extension kit for Large columns P N DU9671 is supplied with this device this overall dimension can go up to 120mm On the pre column holder dry load or pre column 4g up to 300g can be used Set up the column Move up the large black POM holder on the vertical guidance rail Put the PTFE outlet adapter P N DZ5200 at the bottom of the large column 800g and 1600g Place the bottom fitting of the column on the Adapter ETFE or Stainless steel Luer lock female to 28 male located on the stainless steel bulkhead union 90 ee Unscrew lightly the knurled knob in order to allow its vertical movement 4 Move down the large black POM holder Put the stainless steel inlet adapter P N DZ5200 at the top of the large column 800g and 1600g Lock again the black ring of the fitting Adapter PEEK Luer lock male to 4 28 male to the top fitting of the adapter a a Unlock the black ring of the fitting Adapter PEEK Luer lock male to 74 28 male located on the column Kai 211 bis avenue JF Kennedy BP 1140 03100 Montlucon
25. holes and then put the racks on the tray Thanks to notches machined on racks and positioning guides already mounted on the system their emplacement is easy and necessarily proper D 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE MCE ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 www interchim com Bus ZS LASH 8 33 4 70 03 73 01 Positioning notch Well placed rack Back guides Front guides You can also equip your collector with an optional Enhancement tube device for 13 x 100m P N DW3360 It is designed for elevating the racks with small height tubes Its installation is simple too because you have just to emplace it on the collector tray using positioning guides Several models of rack sets are available find a detailed list on APPENDIX 1 It is important to maintain appropriate spacing of the dispensing needle above the tubes A space of 5 25mm between the end of the needle to the top of the tube is recommended To adjust the needle height turn off the system remove the racks and gently move the arm into an easy to access position Do not use the needle to relocate the arm as bending and mis alignment may occur Loosen the set screw and adjust the height of the needle and then tighten the set screw When the system is turned on the arm will return to its home position Collection tubing Dispense arm Dispense head Needle Tightening screw Kai 211 bis avenue JF
26. ile phase and sample The presence of micro particles will cause accelerated wear and tear of the seals Similarly any dried buffer particles on the piston will damage the seal Kai 211 bis avenue JF Kennedy BP 1140 03100 Montlucon FRANCE Wace ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com User Manual interfine interchim fr 6 4 Cleaning replacing a check valve Abnormal pressure fluctuation or the observation of pump noise on the data system Check valve is usually due to check valve problems The noise is regular in nature and is due to pressure and flow variation Pump accuracy is also compromised Details on check valve repairs are given below There are four check valves two per head one for each inlet model 20mm P N LO2620 and one four each outlet model 10mm P N PFS930 If they become dirty they will not open and close correctly for a long time 1 Purge the pump head with a suitable solvent or alcohol 1 propanol or 2 propanol 2 Switch off the module and disconnect it from the electrical supply 3 Remove the plastic cover to gain access to the pump heads 4 Disconnect the Suction and Delivery tubing and fittings 5 Unscrew the check valve housing using a spanner 6 Clean the check valves or provide yourself with news The cleaning allows to remove the particles present in the valves by placing them in an ultrasonic bath with methanol or acetone during approximately five minutes
27. ine are observed Quarterly or when pump head is disassembled Quarterly or when higher noise levels or decreased sensitivity are observed When its efficiency is unreliable Quarterly Daily Weekly or when inaccuracy flow rates solvents leaks or air bubbles are observed As often as possible specially after having pumped aqueous solvents or strong buffers Monthly Kai 211 bis avenue JF Kennedy BP 1140 03100 Montlucon FRANCE Wace ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com Manual interfine interchim fr 8 gt Warranty 8 1 Generalities Interchim guarantees the 292 LASH 00 for one year for parts and labour at the discretion of Interchim in normal conditions of use and installation from the date of signing of the installation report by the service provider approved by Interchim to install the machine and by the customer The PUF LASH 00 device is guaranteed against material and manufacturing defaults in normal conditions of use by approved professionals and within the technical characteristics compatible with the functions defined in the user manual Terms and conditions of the guarantee v The device is only guaranteed if installed by a service provider approved by Interchim The guarantee covers the supply of parts found defective by Interchim free of charge as a minimum within the limits of the parts listed in part 6 2 The device must have been used
28. intenance O Out of warranty Systemi le e NEE Sh ME Ville AC E NR EE EE EEEE eA E E E TE E E P N and description of defective spare e Any further action required touiciicevcazsoxsencedesxcves lt estascsescubeshteycencexueetadssasbes vectutuxssateeentdanebesancesvseanternectsaxtevendnersieenedebaredy RE NOR en Signature gt Service report lt O Installation O End of warranty visit O Warranty O Repair O Maintenance O Out of warranty e ele e ME SL M Module ACTOR icra es eed ence a sede E cau E areas e E cua segsaeeee ade E a dase P N and description of defective spare ln e Any further action ee EI EE RI NAMO enoras aaraa ES Signature gt Service report lt O Installation O End of warranty visit O Warranty O Repair O Maintenance O Out of warranty CSC OS le ME NE E P Ville lei 1 ICS CO E painterchim 211 bis avenue J F Kennedy T l 33 4 70 03 88 55 BP 1140 03100 Montlu on Fax 33 4 70 03 82 60 Hotline 33 4 70 03 73 01 E mail interfine interchim fr Not contractual pictures
29. l movement Thanks to a system pulleys belt a stepper motor drives two special cams on which lean the two pistons Their motion generates a cycle and then creates the suction A static mixing chamber at the outlet ensures a suitable mixing of solvents whatever the working flow rate Moreover an optic sensor can detect leakages in the pump drain pan The pumping is then interrupted and the user informed via the control software The 2 or 4 ways suction valve allows to perform binary or quaternary elution gradients D 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE CTI ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 www interchim com 33 4 70 03 73 01 The injection unit and columns holder system This device gives the possibility to inject sample manually liquid or solid via dry load directly to the chromatography column installed The adjustable height holder of the black POM holder allows to adapt all types of columns A knurled knob can block this holder on the vertical guidance rail The bottom union is fixed Guidance rail Knurled Knob i Pre column o or dry load Column The UV diode array detector This instrument measures the amount of light absorbed by a liquid passing through the cell at a given wavelength It is equipped with a deuterium lamp The two wavelengths and the SCAN function available are selected within a large range from 200 to 600nm or from190 to 840nm in
30. lace the syringe filled with sample on the column and inject manually the desired quantity Unlock the black ring of the fitting Adapter PEEK Luer lock male to 28 Male of the column holder 3 A Screw the fitting Adapter PEEK Luer lock male to a 28 Male adapter Answer yes to the question of the software The method starts Remove the syringe Kai 211 bis avenue JF Kennedy BP 1140 03100 Montlucon FRANCE Wace ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com interfine interchim fr 4 3 Make a solid injection The solid injection is done via a dry load in which the solid sample has been previously deposited The dry load is emplaced directly on the pre column holer An optional separated holder is available to use greater pre columns and have a support comparable to the one of the main column P N PF4530 Injection Mode Equilibration Wait forni Make your injection and then press res In the panel Settings of the method edited select Equilibration in the section Injection Mode According to the type of column chosen a time and a flow rate are written by default they depend on its parameters but you can modified them if need be It is the moment to put the dry load D D 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE Bainterchim _ 1 Unlock the black ring of the fitting Adapter PEEK Luer
31. llector opto sensor Y axis ON OFF switch with EMI filter AC Power supply 230 24V 50W Pump Collector fuses 2 5A 110V x 2 Pump Collector fuses 1 6A 220V x 2 Black POM for column holder fixed x 2 screws Kai 211 bis avenue JF Kennedy BP 1140 03100 Montlucon FRANCE CTI ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com 33 4 70 03 73 01 Part Number PFS400 PFS410 PFS420 PFS430 PFS440 PFS450 PFS460 PFS470 PFS330 PFS480 PFS990 PFS980 102960 N interfine interchim fr User Manual Item Part Number N Column holder TEFZEL ferrule with Stainless steel lock ring for 1 8 tubing GV1690 PEEK 28 long nut for 1 8 tubing for GV1690 GV1710 Adapter Luer Lock Male to 28 Male PEEK JO2630 Guidance part for large black POM holder FO4400 Stainless steel union for flash configuration FV6560 Large black POM injection holder grub screw 104960 Stainless Steel bulkhead union 90 PES370 9 Adapter ETFE Luer Lock Female to 4 28 Male or PFS780 2 Adapter Stainless Steel Luer Lock Female to 1 4 28 Male GV1680 2 PEEK Ring Outlet column holder 102690 3 Binding column 102700 6 D 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE MCE ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 www interchim com mm 33 4 70 03 73 01 Item Part Number Pre column holder TEFZEL ferrule with Stainless steel lock ring for 1 8 tubing GV1690 PEEK 1 4 28 long nut for
32. lvent Tray with a drainage system Serial communication cables 2 x Sub D9 male female 1 x Sub D9 female female v Distribution power cables 2 x main voltage supply 1 x 24V v Binding column General power cable model according to the destination country v USB key including the file of installation exe of the freeware Interchim Software Demo Stylus Keyboard v Documentation User Manual for PUR LASH 00 system Instruction Manual for Interchim Software Adjusting Protocol of Assembled Unit for Flash 06 DAD UV detector PDF document Operational Qualification and test report My documents Quick start for Interchim Software D D a 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE TAC re 33 470 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com PUG LASH 8 33 4 70 03 7301 3 gt Installation 3 1 Assembling the system Select a suitable location by ensuring adequate air space 15cm around the system to provide ventilation of the controller the pump the detector cooling fans Its total weight is approximately 60kg Air extractions v Assemble the system in the following order Fraction collector 25kg Unit control 15kg Detector 7kg Pump 8kg Column Holder 3kg Solvent tray 1kg Put then the stainless steel solvent tray supplied on top to dispose solvents up to five They cover the totality of the system with a drainage sy
33. mp electric switching valve 200mL mm 30 bar P N FY9960 14 CarouXel P N JO3750 15 Autosampler P N LO8850 16 Mass Spectrometer interface P N JO1090 17 2 fraction collector coupled P N FK6140 18 Ballasting kit for 3 16 and 1 8 tubing P N FV1290 19 Racks set 20 Stand Alone column holder for flash P N PF4530 D 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE MCCAIN ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 www interchim com PURE LASH SOC 33 4 70 03 73 01 Designation Items 1 Solvent level sensors 5 1 global Communication interface 5 1 cable Sensors cables x 5 Straps 80cm x 5 Removable cable ties x 5 Power adapter Hardware Installation procedure 1 Solvent level sensors 5 5 Separated Communication interface 5 5 cable Sensors cables x 5 Straps 80cm x 5 Removable cable ties x 5 Power adapter Hardware Installation procedure 2 Bar code reader Reader with USB connection Bracke Hardware Installation procedure 3 Enhancement tube device for 13 x 100mm tubes 4 Video camera TLC plates holder Webcam with USB connection Bracket CD for Webcam drivers installation TLC plates holder Hardware Installation procedure 5 Fume enclosure Side panel Transparent door 5 screws Collar Magnetic flap Hardware
34. nce 1 accuracy with H O degassed at 20 C Repeatability better than 0 5 v Gradient former Linear response from 2 to 98 Injection Liquid manual loop or Solid v System protection Software Pressures Min Max adjustable Leakage detection v Maximum pressure 10 bar 145 psi v Control via PC software INTERCHIM SOFTWARE VX X v Storage up to 32 methods in software memory plus 160Go of hard disk memory v AC mains supply voltage 220 240VAC 50 60Hz or 110 120VAC 50 60Hz Electrical protection Delayed action fuse 3 15A or 6 3A v Power distribution TT or TN power system only transient over voltages according to installation category II Class 1 equipment requires mains socket plug with protective earth terminal v IP Code IP20 v Dimensions 680 x 600 x 560mm Width x Height x Depth without connection v Weight 60kg v Temperature 5 to 40 C v Others Indoor use Altitude up to 2000m Pollution degree 2 Noise level lt 35dB D 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE MCCAIN ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 www interchim com d 33 4 70 03 73 0 As mentioned in the chapter 3 5 Prepare the fractions collection several racks are usable with the fraction collector Here is a recapitulative list indicating their designation the features of tubes used and the total capacity of the rack set SHORT RACKS a rack set includes 4 racks Designation Tubes si
35. on O End of warranty visit O Warranty O Repair O Maintenance O Out of warranty Systemi le e NEE Sh ME Ville AC E NR EE EE EEEE eA E E E TE E E P N and description of defective spare e Any further action required touiciicevcazsoxsencedesxcves lt estascsescubeshteycencexueetadssasbes vectutuxssateeentdanebesancesvseanternectsaxtevendnersieenedebaredy RE NOR en Signature gt Service report lt O Installation O End of warranty visit O Warranty O Repair O Maintenance O Out of warranty e gl e e ME SL M Module ACTOR icra es eed ence a sede E cau E areas e E cua segsaeeee ade E a dase P N and description of defective spare ln e Any further action ee EI EE RI NAMO enoras aaraa ES Signature gt Service report lt O Installation O End of warranty visit O Warranty O Repair O Maintenance O Out of warranty CSC OS le ME NE E P Ville lei 1 ICS CO E gt Service report lt O Installation O End of warranty visit O Warranty O Repair O Maintenance O Out of warranty Systemi le e NEE Sh ME Ville AC E NR EE EE EEEE eA E E E TE E E P N and description of defective spare e Any further action required touiciicevcazsoxsencedesxcves lt estascsescubeshteycencexueetadssasbes vectutuxssateeentdanebesancesvseanternectsaxtevendnersieenedebaredy RE NOR en Signature gt Service report lt O Installation O End of warranty visit O Warranty O Repair O Maintenance O Out of warranty e ele e
36. option P N JO5140 Deuterium lamp de Lp TE j 7 7 Li Flow cell E S F A Kai 211 bis avenue JF Kennedy BP 1140 03100 Montlucon FRANCE Wace ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com User Manual interfine interchim fr The fraction collector The fraction collector is placed downstream of the detector After the detection of the signal chromatogram the results obtained at the outlet of the column peaks can be directed to tubes disposed on racks A built in 3 way valve allows to choose between the collection or the rejection of the liquid Needle Electronic board collector The computer and its touch screen The system is controlled by the central processing unit via a touch screen which replace the keyboard and the mouse The operator uses the control software and can create methods to run the instrument Touch screen USB Ports Power light D 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE MCCAIN ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 www interchim com PUR LASH COC 33 4 70 03 73 01 This module contains the main ON OFF switch and centralizes the connection for data and power supply to the other modules Four USB connectors are also available 1 in front of the system under the screen and 3 to the left hand side cable maximum length 2m The external connections Some others external connectors allow to
37. rews indicated below using an Allen wrench 2 5mm Take care not to lose M4 flat washers 6 Put the detector in the upright position and take off the front plastic cover 7 Then unscrew the two M4 domed head screws located on the back of the module using an Allen wrench 3mm and take off the top sheet cover 8 Loosen the screw on the socket of light tube behind the cell compartment using T 20 Torx adapter Turn the lamp socket to the horizontal position and tighten the same screw to fix it again 9 Take the lamp power supply cables and disconnect the 3 pin white connector Loosen and remove the two screws which hold the lamp using T 10 Torx adapter Kai 211 bis avenue JF Kennedy BP 1140 03100 Montlucon FRANCE Wace ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com interfine interchim fr User Manual Never touch quartz bulb of the lamp with bare fingers to avoid damages causing faulty working of the detector 10 Remove the lamp from the lamp socket and provide yourself with a new one 11 Carefully insert the new lamp assuring that it is correctly positioned Should you touch the glass of the lamp accidentally clean it thoroughly with a lint free cloth and alcohol 12 Screw it in securely and connect the plug in the 3 pin white socket 13 Reassemble the unit by making the same steps but in the reverse order After the lamp replacement recalibration and zeroing of the counter of opera
38. rmed on a routine basis to ensure long term safe and trouble free operation of the system The frequency of the maintenance activities is dependent on the nature of the application the solvents used the quantity of the mobile phase delivered by the pump the level of cleanliness of the facility etc The pump has been designed for reliability and needs very little routine maintenance when Operated correctly The following figure represents the liquid connections of the pump ess 2UCtION tubing _ Check valve housing Delivery tubing Tee union I Bulkhead union Reduced accuracy of the pump may be due to seal wear or to a fault in the check valves This section provides information and procedures about how to replace consumable parts and how to maintain the instrument The removal of protective panels may only be performed by an authorized person 6 2 Removing a pump head When disassembling or reassembling the pump make sure that each component is clean and take care that the system is assembled in a clean environment Remove it if you need to replace its seal details hereafter 1 Purge the pump head with a suitable solvent or alcohol 1 propanol or 2 propanol 2 Switch off the module and disconnect it from the electrical supply 3 Remove the plastic cover to gain access to the pump heads 4 Disconnect the Suction and Delivery tubing and fittings 5 Remove the two screws Allen wrench 3 mm Carefully unscrew
39. screw it in a stainless steel piece which serve of weight to maintain the tubes at the bottom of the containers PEEK nut Ballast Position the solvents containers on the solvent trays for instance and put the ballasted tubes into them GRADIENT VALVE The gradient valve to Tee Pump inlet tube is already connected into the pump I 28cm 3 16 ID 3 2mm D 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE CTI ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 www interchim com PUR LASH 33 4 70 03 73 01 Pump to pre column holder Using the 60cm x 2 4mm ID tube insert one end into the mixing chamber and the other end into the pre column holder inlet Position the tubing seat the ferrules and hand tight each fitting Pre column holder to column holder Using the 90cm x 2 4mm ID tube insert one end into the pre column holder outlet and the other end into the column holder inlet Position the tubing seat the ferrules and hand tight each fitting Column holder to detector Using the 70cm x 2 4mm ID tube insert one end into the column holder outlet of the stainless steel bulkhead union 90 and the other end into the detector inlet Position the tubing seat the ferrules and hand tight each fitting Detector to fraction collector Using the 30cm x 2 4mm ID tube insert one end into the detector outlet and the other end into the fraction collector inlet Seat the ferrules and hand tighten Waste Usin
40. stem and their design avoids any leakage risk Kai 211 bis avenue JF Kennedy BP 1140 03100 Montlucon FRANCE Wace ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com User Manual interfine interchim fr 3 2 Install the tubing and prepare the containers The tubes situated outside the housing are delivered apart in order to avoid all deterioration during transfer they are already equipped with the suitable fittings nut ferrule to connect them easily to the system Regarding the main circuit two sizes of tube are used 3 16 ID 3 2mm for suction 1 8 ID 2 4mm for others Follow the instructions below to correctly install the tubing Suction Provide yourself with the two or four Suction tubes I 1 5m 3 16 ID 3 2mm supplied they are more brittle than others so take care not to bend them For the tube A take the extremity where there is a fitting with a long spring and put the ferrule in the PEEK body of the suction valve Screw the PEEK nut tighten it hand tight and position properly the long spring Renew the same operation for B C and D as indicated on the photo Gradient valve body PEEK nut Outlet To ensure the pumping continuity of the solvents and avoid the air entry in the hydraulic circuit the others extremities can be equipped with ballasts in option P N FV1290 It consists in fact to put a fitting nut set ferrule and
41. ting hours must be carried out cf Service Info 39 6 6 Cleaning the detector flow cell A contaminated cell in lowered light transmission may increase noise level and make Flowcell difficulties at zeroing The simplest cleaning method is flushing dismantled cell with suitable solvents The cell must be taken out from the instrument before washing 1 Follow the instructions 1 to 7 described in the previous chapter 6 5 Replacing detector deuterium lamp in order to properly disassemble the system 2 Disconnect the outlet tubing from the cell PEEK nuts 3 Remove easily the cell from the system by tilting the locking lever placing one hand under the cell to prevent it from falling to the bottom and tilting the locking lever with the other hand 4 Select the solvent type according to character of contamination by a series of mutually miscible solvents It is possible to use both organic and inorganic solvents and diluted solutions of acids 5 Flush the cell with pure solvent and rinse extensively 6 Reassemble the module by carrying out the Locking same steps but in the reverse order Flow cell O lever After the operation check cell cleanness purging r the system and verify there is no baseline drift D 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE CTI T l 33 4 70 03 88 55 Fax 33 4 70 03 82 60 www interchim com ES 7 gt Preventive measures 33 4 70 03
42. to the mains knowing that the connector depends on the destination country comply with country regulations in effect Kai 211 bis avenue JF Kennedy BP 1140 03100 Montlucon FRANCE Wace ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com User Manual For American system 110 120VAC 50 60Hz 6 2A delay action fuses 2 x 6 3A For European system 220 240VAC 50 60 Hz 3 1A delay action fuses 2 x 3 15A interfine interchim fr ON OFF Switch Fuses Drawer Power Cable Check that all the ON OFF switches of secondary modules pump detector collector are on 1 and switch on the unit with the main ON OFF switch 3 Options and accessories 1 Solvent level sensors 5 1 global P N FSP720 or Solvents level sensors 5 5 Separated P N FSP730 2 Bar code reader P N GO2380 3 Enhancement tube device for 13 x 100mm tubes P N DW3360 4 Video camera TLC plates holder P N GO2390 5 Fume enclosure P N PF4350 6 Quad system P N PF4360 7 2 acquisition channel P N PF4320 8 FLASH ELSD P N F10320 9 Funnel rack set 1 unit P N FJ4331 or Funnel rack set 4 units P N FJ4330 10 Safety solvent caps kit tubing 3 16 1 unit P N JO1620 11 Safety waste cap with container 20L filter P N JO4500 12 Drainage system of the tray collector P N DZ7790 13 Injection pu
43. x 90 kit LV9790 Detector Flow cell 0 3mm HO7270 1 year 6 months Deuterium lamp PFS970 or 1000 hours gt Terms of use Use of clean solvents and clean containers Respect precisely the maintenance procedures gt Damages not covered Damaged piece due to wrong use or replacement or inappropriate solvent pumping Blocked passage due to uncleanliness of installation or unsuitable mobile phase D 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE MCCAIN ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 www interchim com User Manual 33 4 70 03 73 01 9 gt List of spare parts Blow up views Item Part Number N Fittings unions amp tubing TEFZEL ferrule with Stainless steel lock ring for 1 8 tubing GV1690 PEEK 1 4 28 short nut for 1 8 tubing for GV1690 GV1700 PEEK 1 4 28 long nut for 1 8 tubing for GV1690 GV1710 PEEK 5 16 nut ETFE ferrule for 3 16 tubing JO8520 Adapter Luer Lock Male to 1 4 28 Male PEEK JO2630 Adapter ETFE Luer Lock Female to 1 4 28 Male PFS780 Adapter Stainless Steel Luer Lock Female to 1 4 28 Male GV1680 PEEK tee union for 3 16 tubing LV9960 Suction tube FEP 1 50m 3 16 ID 3 2mm 1 PEEK nut LV9830 1 ETFE ferrule x 2 Suction tube FEP 1 50m 3 16 ID 3 2mm 1 PEEK nut LV9840 1 ETFE ferrule x 4 Tube Gradient valve to Tee pump inlet FEP 28cm LV9850 3 16 ID 3 2mm 2 PEEK nuts 2
44. ze Tubes volume Tubes by rack Tubes by rack set Total volume PF4390 18 x 150mm 18 x 180mm PF4370 13 x 100mm PF4380 16 x 150mm PF4410 21 x 150mm PF4420 25 x 150mm PF4430 28 x 150mm PF4440 PURIFLASH OU 29 5 x 200mm 800 ml min up to 10 bar Kai 211 bis avenue JF Kennedy BP 1140 03100 Montlucon FRANCE Wace ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com gt Service report lt O Installation O End of warranty visit O Warranty O Repair O Maintenance O Out of warranty Systemi le e NEE Sh ME Ville AC E NR EE EE EEEE eA E E E TE E E P N and description of defective spare e Any further action required touiciicevcazsoxsencedesxcves lt estascsescubeshteycencexueetadssasbes vectutuxssateeentdanebesancesvseanternectsaxtevendnersieenedebaredy RE NOR en Signature gt Service report lt O Installation O End of warranty visit O Warranty O Repair O Maintenance O Out of warranty e ele e ME SL M Module ACTOR icra es eed ence a sede E cau E areas e E cua segsaeeee ade E a dase P N and description of defective spare ln e Any further action ee EI EE RI NAMO enoras aaraa ES Signature gt Service report lt O Installation O End of warranty visit O Warranty O Repair O Maintenance O Out of warranty CSC OS le ME NE E P Ville lei 1 ICS CO E gt Service report lt O Installati
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