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COOLSCOPE II User`s Manual>

1. Memory Macro image Status bar Exposure information Date and time level meter The status bar shows the exposure information level meter date and time NOTE Buttons that may be used are highlighted in yellow when the mouse pointer is positioned over them Buttons highlighted in red cannot be used Be ser III Description of the Multi Screen EPE Names and Functions of Buttons gj ax 2 nea ve 28 mae e lols Magnification 1 0n DA gt mm EE o Gwm C FO03 1 MAGEOO0 BMP In the descriptions given on the following pages all mouse click operations are left click operations unless specified as right click operations Tool buttons Observation buttons Digital Zoom Image capture buttons Control buttons 296 2 III Description of the Multi Screen Tool buttons 1 ENLARGE button Enlarges the macro image or cancels its enlargement DISPLAY button Switches between Multi screen and Full screen 3 CONTROLLER button Closes the controller May be used with FULL screen Right click on the screen to display the controller again HELP button Opens closes the help screen P 43 5 SCALE button Displays hides the scale Right clicking changes the color of the scale CROSS LINES button Displays hides focusing lines on the macro image Right clicking changes the color of the cross lines SETTING button Displays the Detai
2. Taking out the specimen slide Vv Shutting down the COOLSCOPE Contents When a specimen slide is placed on the tray the loaded image will be displayed Micro image MOVE button MAG button FOCUS button AF butt o mE ptr SAVE button POWER OFF button Macro image EJECT button 6 Click on a desired observation position on the macro image 7 With 5X selected as magnification click on the AF button 8 To move the viewing position follow the steps described below e Click on a desired position on the micro or macro image e Click on the MOVE button 9 To change magnification click on the MAG button 10 To adjust the image focus click on the FOCUS button 11 Insert the CF card into the COOLSCOPE and click on the SAVE button wv Please refer to Chapter V Basic Image Capture Tasks for more information 12 Click on the EJECT button and take out the specimen slide Close the tray by clicking again on the EJECT button Z caution You cannot manually push the tray into the COOLSCOPE 13 Shut down the COOLSCOPE by clicking on the on screen POWER OFF button NOTE The COOLSCOPE cannot be shut down unless the specimen slide is first removed 19 I Connecting Equipment Connecting Equipment 1 Connect the display or projector to the COOLSCOPE s MONITOR terminal 2 Wind one turn of th
3. he other side NOTE e This step is not required when you save your image to an FTP server Note however that in this case you need to set up the FTP server in advance Please refer to Chapter VIII Networking Functions for more information on the FTP setup procedure e When a USB memory is set the COOLSCOPE detects the device and the SAVE button and items in SAVE TO area change to the appropriate condition automatically 36 V Basic Image Capture Tasks Notes on Using an External Recording Medium Images can be saved to or reproduced from a medium CF card USB memory Pay attention to the following when using a medium e Use a Type I or Type II compliant CF card microdrive or a USB I F memory e Carefully read the users manual provided with your medium before using the card e To format a CF card on your computer use FAT12 FAT16 Sometimes these are expressed simply as FAT or FAT32 Depending on the OS used you may not be able to select a format e Insert the connecting side with the connecting slot of a CF card into the card slot with the back label side facing toward you Carefully note the direction and inclination of a CF card when inserting e Check to see that the CF card indicator LED is off before inserting or removing a CF card e Do not remove a CF card or turn off power to the COOLSCOPE while the card is being accessed the LED indicator is on Attempting to do so may result in lo
4. Prompt ij Script Activex controls marked safe for scripting Disable Enable Prompt E Downloads as Automatic prompting For file downloads Disable Enable fae rie J 1 4 lt Reset custom settings Custom Level Default Level Reset to Medium 3 Disable personal firewall blocking Some operating systems or antivirus software such as Virus Buster may activate a personal firewall To prevent this either disable the personal firewall or modify blocking to enable reception via UDP port 12102 which is used by this device For instructions on changing the settings refer to the documentation for your operating system or antivirus software If ROOM1 is not displayed make sure any personal firewall of the operating system or antivirus software is not preventing it 111 VIII Networking Functions 3 3 6 Java Applet Settings Java applets must be enabled to use ROOM2 This procedure varies depending on your browser and operating system For Windows and Internet Explorer 1 Change the browser settings e In Tools select Internet Options A Welcome Microsoft Internet Explorer File Edit view Favorites IRE Help Mail and News gt i A yy T gt ae 2 Q Back gt x Pop up Blocker p PS 4 S ae BS eae Manage Add ons Address http 128 31 30 3 herein Windows Update Windows Messenger Diagnose Connection Prob
5. See the picture below for connection Connecting the COOLSCOPE directly to the PC COOLSCOPE Third party 1OBASE T 100BASE TX cable crossed 1 Connect the display or projector to the COOLSCOPE s MONITOR terminal 2 Connect the mouse to the COOLSCOPE s USB H terminal 3 Connect the COOLSCOPE s LAN terminal and the PC s LAN terminal with a cross cable 4 Connect the power cord to the COOLSCOPE s AC inlet 278a VIII Networking Functions Connecting the COOLSCOPE to a network COOLSCOPE Network Third party 1OBASE T 100BASE TX A cable straight A Third party 10BASE T 100BASE TX cable straight 1 Connect the display or projector to the COOLSCOPE s MONITOR terminal 2 Connect the mouse to the COOLSCOPE s USB H terminal 3 Connect the COOLSCOPE s LAN terminal and the hub s terminal with a straight cable 4 Connect the power cord to the COOLSCOPE s AC inlet NOTE When the COOLSCOPE is used across a network the screen of the COOLSCOPE must the Multi screen initial screen or the Full screen If a help is shown for an image of a medium correct images cannot be acquired across the network JO VIII Networking Functions 3 Using the COOLSCOPE via a Network You can connect the COOLSCOPE and a PC in a network and operate the COOLSCOPE from the PC or exchange image files between the COOLSCOPE and the PC There are three methods for operating the
6. 15 Line list box Macro image 93 VIII Networking Functions 1 RELOAD button 2 WB button 3 MOVE button 4 MAG button 5 AF button 6 FOCUS button 7 DIGITAL ZOOM button 8 APERTURE button 9 AE button 10 Extended Mode check box 11 DOWNLOAD button 12 TYPE list box 13 SIZE list box 14 QUALITY list box 15 Line list box 16 INTERVAL TIMER list box 17 Micro Size list box Names and functions of buttons and list boxes Update the micro image Adjust the white balance Move the observation position Change the magnification Bring the image into focus Adjust the image focus Electronic zoom Adjust the stop Adjust the image brightness Allow enabling disabling of the Extended Mode function Retrieve the micro image from the COOLSCOPE Allow selection of an image file format at the time of micro image retrieval Allow selection of an image size at the time of micro image retrieval Allow selection of a JPEG compression ratio at the time of micro image retrieval Change the color of the cross line on the macro image Allow the selection of intervals for updating the micro image Select the size of the micro image 94 VIII Networking Functions The ROOM 1 screen can be used only with the Internet Explorer 6 02 SP2 on the Windows 2000 SP4 or Windows XP SP2 When multiple PCs access one COOLSCOPE simult
7. Gain levels below 100 will result in a reduced output saturation level Gains in excess of 100 may be set Density button CHR Enables selection of a color density in a range of 50 to 50 Left clicking on the button increases the adjustment value while right clicking on it reduces the value Select a positive value to make the displayed color darker and a negative value to make it lighter Hue button HUE This button is used to change hues during fine color adjustment Make adjustments while viewing the image Do not forget to restore the original hue 56 15 16 17 18 VII Details Menu Black level setting button SFT Select a negative value when removing flares and the like from dark areas of the screen or when making black areas sharper Tone selector buttons TON1 to TON4 LINR TON6 TON7 UTN1 to 7 Seven options are available The contrast changes with each option Select a tone that suits your observation while checking it on the monitor TON1 Wide dynamic range Should be selected for a specimen with high contrast requiring shades of gray from dark to bright areas TON2 Low contrast Should be selected when low contrast is required Recommended specimen Cells tissues and general subjects TON3 Standard contrast Should be selected for normal image capture Recommended specimen Cells tissues and general subjects TON4 High contrast Should be selected when high contrast is req
8. This dialog box is an example for the Internet Explorer Other dialog box appears for other browser Note that some browsers do not have the Always trust option In such cases this dialog box appears every time when displaying the ROOM 2 96 VIII Networking Functions 17 Micro Size list box 16 Interval Timer list box Interval Timer Micro Size 320 240 v Macro image Micro image Focus D zoom 7 Aperture F AE O Extended Mode Pick Up Image DownLoad Tye bmx Size Middle 640x480 v Controller 1 RELOAD button 2 WB button Nikon Wocl COOLSCOPE I WB 3 MOVE button Magnification Be 10 20 a 4 MAG button 5 AF button AF 6 FOCUS button 7 DIGITAL ZOOM button 8 APERTURE button 9 AE button 10 Extended Mode check box 11 DOWNLOAD button 12 TYPE list box 13 SIZE list box 14 QUALITY list box Line 15 Line list box Names and functions of buttons and list boxes 1 RELOAD button 2 WB button 3 MOVE button 4 MAG button 5 AF button 6 FOCUS button 7 DIGITAL ZOOM button 8 APERTURE button 9 AE button 10 Extended Mode check box 11 DOWNLOAD button 12 TYPE list box 13 SIZE list box Update the micro image Adjust the white balance Move the observation position Change t
9. for the setup procedure of the IP address of the COOLSCOPE e Whether an OS and an FTP server are installed in the PC for the FTP server The PC that has the FTP server function is required to save the image from the COOLSCOPE to the FIP server Check that the PC to be used as a server has the following applicable OS Applicable OS Windows 2000 SP4 Windows XP Professional SP2 Mac OS X ver 10 3 or later 83 VIII Networking Functions 3 2 2 Setting Essential Items Network Settings In this instruction manual explanation are given assuming that the IP address of the COOLSCOPE is 192 168 10 2 that of the server is 192 168 10 1 and that of the subnet mask is 255 255 0 0 while the gateway address is 192 168 128 1 Of course you should use the IP address assigned to you by your network administrator We must assume that network settings on the PC side LAN connection are complete Please consult your network administrator for details Network settings of the COOLSCOPE If you are going to connect the COOLSCOPE to a LAN you need to enable it for network operation and set its own address To make network settings on the COOLSCOPE select Network Settings from the SETUP menu to call up the Network Settings screen shown below PowerOn Sett ings NetWork Settings File Settings Additional Return to Window NetWork Settings al Enable NetWork Read Only FIP WEB disable Write Control Enables
10. 1 Enter the IP address of the COOLSCOPE from the MS DOS prompt command prompt of Windows to connect to the COOLSCOPE Example FTP 192 168 10 2 2 Execute the Is command to search for a directory named RAMIBOX 3 Execute the cd RAMIBOX command The following file names will be displayed e IMG bmp e IMG jpg e IMG tif e MACRO bmp e MACRO1 jpg e MACRO2 jpg The number of image size such as 2560 or 1280 is indicated in the space marked with Time stamps of these files are O hours O minutes Acquired files will be given the time of the date when you issue the Get command 4 Execute the Get command for one of the files and the latest image that is in the COOLSCOPE at that point will be downloaded to the PC a If the PC and the COOLSCOPE are connected using a USB cable and the access is frequent the capture of the image file may result in failure In that case temporary halt the access via USB cable i The macro image can be written in the MACRO bmp Also the right half of the macro image can be written in the MACRO1 jpg and the left half can be written in the MACRO2 jpg Caution IMG2560 bmp IMG2560 jpg and IMG2560 tif may be used only in Multi screen mode initial display mode or Full screen mode Images cannot be captured properly when Help is running or an image from the CF card is being displayed A detailed command list is available from Nikon If you wish to have one pleas
11. EJECT button a on screen e Press the gray tray OPEN CLOSE switch Press the switch slowly EJECT button Tray OPEN CLOSE switch The tray opens A N Caution Keep clear of the entrance exit opening and do not look into it 2 Open the specimen slide holder and place a specimen slide with the cover glass facing specimen EE lid up on the tray oe Place it properly making sure that it lies flat spedmen slide holder NOTE To indicate information of a specimen write it down in the area of the white paper label on the specimen slide The area is located nearside and has an inside height of 15 mm Or use a Specimen slide with white frost processed surface and write the information with a black pen 3 Hold down the specimen slide with the holder Check that the holder is closed and the specimen Slide is properly fastened 4 Press the gray tray OPEN CLOSE switch at the front of the COOLSCOPE or click on the EJECT button on screen At this time the tray is retracted into the COOLSCOPE The image loading starts automatically when a specimen slide is placed in the COOLSCOPE A N Caution You cannot manually push the tray into the COOLSCOPE rE II Starting up and the Shutting down the COOLSCOPE 5 To take out the specimen slide follow the same procedure as described in Step 1 and take the specimen sli
12. NOTE The RUN button becomes a Pause button when updating of the micro image starts Enabling disabling the ANNOTATION function ROOM 3 When the ANNOTATION function is enabled the position clicked on the micro image on the PC using the X mark can be indicated on the COOLSCOPE s micro image 1 Check the ANNOTATION check box to enable the ANNOTATION function 2 Uncheck the check box to disable the ANNOTATION function Caution Position information information on the position clicked on the macro or micro image on the PC is calculated with the upper left corner of the image on screen as the origin and is transmitted to the COOLSCOPE As a result the observation position is changed on your PC by clicking on the image with the upper left corner of the macro or micro image not shown on the screen the clicked position will differ from that of the updated mouse pointer For instance if you click on e on your PC when the macro image is displayed as shown below the mouse pointer will appear at the x position Remember that in this case the mouse pointer does not appear at the clicked e position Image displayed on the PC The shaded area of the COOLSCOPE macro image is hidden 108 VIII Networking Functions 20 Enabling the display function of the ANNOTATION Cursor ROOM 3 When enabling the display function of the cursor the location of the mouse pointer of the COOLSCOPE can be displayed wit
13. ml a O eee Checking for presence in a LAN lt Using a command from PC to search for the COOLSCOPE in a LAN gt Check network connections by using the ping command e For Windows select Start Programs Accessories Command Prompt in that order and enter the command from the prompt given below e Mac OS X select Macintosh HD Applications Utilities Terminal and enter the command from the TERMINAL screen Example If the IP address of the COOLSCOPE is 192 168 10 2 Enter the command ping 192 168 10 2 and hit the return key If a message such as Reply from 192 168 10 2 bytes 32 time is returned it means that the presence of the COOLSCOPE in a LAN is recognized The problem thus lies in the settings of the COOLSCOPE or PC If a Request timed out message is returned the presence of the COOLSCOPE is not recognized Check the cables hub connections and the network settings of the PC e g the IP address Also check that the PC is connected to a LAN by using a browser or the ping command Set exception items in the browser s proxy settings Checking the set values lt Settings on the COOLSCOPE gt e Make network settings in Network Settings of the COOLSCOPE SETUP menu e g Enable Network and IP Address Always be sure to make gateway settings if data communication between different network groups is to be performed such as sending or receiving data to and f
14. s USB H terminal Connect the power cord to the COOLSCOPE s AC inlet Ferrite core USB H terminal MONITOR terminal CF card slot Caution Be careful about the orientation of the CF card Face the back side upward he other side 17 Basic Operation of the COOLSCOPE Starting up the 1 COOLSCOPE 1 Set the main power 2 Press the power switch to the position switch After a moment the multi screen appears Placing a specimen slide in the COOLSCOPE 3 Click on the EJECT button The COOLSCOPE tray opens 4 Place a specimen slide on the tray NOTE1 Open the specimen slide holder and place a specimen slide on the tray with the cover glass facing up Position it properly making sure it is not tilted NOTE2 To indicate information of a specimen write it down in the area of the white paper label on the specimen slide The area is located nearside and has an inside height of 15 mm Or use a specimen slide with white frost processed surface and write the information with a black pen 5 Close the tray by clicking on the EJECT button again Caution You cannot manually push the tray into the COOLSCOPE 18 Selecting an observation position Ge Vv Bringing the image into focus Gece Observing the specimen e Vv Capturing the image Vv
15. we NOTE Changing the white balance automatically resets the camera gain to Y100 R100 and B100 After setting the white balance if necessary call up the camera setting suitable for the specimen as described in 5 1 The adjusted white balance setting is applicable only for the conditions used in the adjustment such as the magnification and the size of the diaphragm If the magnification or the size of the diaphragm is changed adjust the white balance again 5 3 Locking the automatic exposure You can lock the automatic exposure The COOLSCOPE adjusts its exposure at all times to achieve the optimal image brightness This adjustment can be stopped for fixed exposure 1 Click on the AEL button The AEL button turns green if auto exposure is locked 5 4 Displaying thumbnail images You can display images saved on the CF card as thumbnails 1 Click the Thumbnail button The screen changes to the VIEW menu in the Details menu showing thumbnails of images stored in the most recent folder inside the NIKON folder on the CF card 50 VII Details Menu Details Menu Clicking on the SETTING button displays the Details menu The Details menu enables the performance of a variety of tasks including adjusting the camera splitting the screen deleting images from the medium and setting up the network ox CMP AE GAIN 100 R100 CHRM HUE SFT e The Details menu contains three menus CAM VIE
16. 1 interface board or a USB 1 1 hub the COOLSCOPE units do not operate normally If connecting multiple units of COOLSCOPE is desired use a USB 2 0 interface board and a USB 2 0 hub or use Windows XP for the OS The COOLSCOPE does not come with a USB cable Be sure to purchase an appropriate cable that suits the USB standard of the PC or hub that will be used e USB 1 1 or USB 2 0 compliant cable when the PC or hub is USB 1 1 e USB 2 0 compliant cable when the PC or hub is USB 2 0 The COOLSCOPE may not operate reliably if you use cables other than those specified 130 3 7 2 VIII Networking Functions USB host drivers Use the USB host drivers made by Microsoft Corporation as the drivers included with your interface board may not operate properly e When using the USB 1 1 interface board The driver is included with the OS e When using the USB 2 0 interface board The driver is included with the OS providing it is Windows XP Service Pack 1 SP1 or newer If you are using Windows 2000 or Windows XP prior to SP1 download the USB 2 0 driver from the Microsoft Windows Update Web site Visit the Microsoft website for details on how to download drivers To install the USB 2 0 host driver that you have downloaded read the manual supplied with your interface board or consult the manufacturer Connecting the COOLSCOPE and a PC Follow the procedure described below to connect the COOLSCOPE and a PC 1 Turn on the po
17. 16A VCTF 3x0 75mm 3m long maximum rated at 125V AC minimum 165 XIII Specifications and Confirming Standards Temperature 0 C to 40 C Humidity 85 RH max Altitude 2 000 m max Operating environmental conditions Pollution degree Degree 2 Installation Category II Electric shock protection class Class I Indoor use only Storage environmental Temperature 20 C to 60 C conditions Humidity 90 RH max no condensation Outer dimensions and Outer dimensions 184 mm W x 358 mm H x 383 mm D not including weight protrusions Weight Approx 18 5 kg e UL Listed Product Compliance with which UL standard UL61010A 1 e FCC 15B Class A satisfied This equipment has been tested and found to comply with the limits for a Class A digital device pursuant Part 15 of the FCC Rules These limits are designed provide reasonable protection against harmful interference when the equipment is operated in a commercial environment This equipment generates and can radiated radio frequency energy and if not installed and used in accordance with the instruction manual may cause harmful interference to radio communications Operation of this equipment in residential area is likely to cause harmful interference in which case the user will be required to correct the interference own expense Canadian Standard This class A digital apparatus complies with Canadian ICES 003 Cet appareil num rique de la classe A est confo
18. Avoid locations in areas of dust e Make sure that the temperature is O to 40 and the humidity is 60 or less Installing the COOLSCOPE in a hot and humid location may cause mildew formation or condensation resulting in degraded performance or malfunction e Do not install the COOLSCOPE against the wall Leave space of at least 10 cm from the wall Be sure to also provide 10 cm of space on top of the COOLSCOPE e Do not install the COOLSCOPE in a locker or cabinet e Select a layout that allows easy detachment of the power cord from the COOLSCOPE s AC inlet in the event of emergency Safety Precautions 6 Observable specimens Specimens observable with the COOLSCOPE must consist of a single cover glass equipped specimen Slide of the following size e Thickness 1 7 mm or less glass slide thickness specimen thickness excluding cover glass thickness e Glass slide Be sure to use a JIS R3703 or ISO 8037 compliant glass Thickness 0 9 to 1 2 mm Width 25 to 26 mm Length 75 to 76 mm Cover glass Specimen gt ee gt s o P 0 a 1 7 mm or less Glass slide e Cover glass Be sure to use a JIS R3702 or ISO 8255 compliant glass JIS R3702 Thickness No 1 0 12 to 0 17mm No 1 S 0 15 to 0 18mm ISO 8255 Thickness No 1 0 13 to 0 17mm No 1 H 0 15 to 0 17mm Exercise caution with unoccluded temporary specimen slides as these can stain the inside of the COOLSCOPE Be careful to prevent the occluding ag
19. Caution In certain cases CF cards formatted in a format other than FAT32 or NTFS may not be successfully reformatted to FAT16 Label Label illumination To turn on the illumination for the label on the specimen slide check the box NOTE To indicate information of a specimen write it down in the area of the white paper label on the specimen slide The area is located nearside and has an inside height of 15 mm Or use a specimen slide with white a frost processed surface and write the information with a black pen 3 When you are finished click on the OK button The changes made to the settings are stored closing the Settings screen and the on screen software keyboard Click on the CANCEL button if you wish to cancel the changes 3 Capturing an Image 1 Click on the SAVE button The image is saved to the medium CF card or USB memory or FTP server NOTE e During the first image saving following placement of a specimen slide on the tray a macro image of the specimen is saved at the same time The macro image s file name contains a folder name followed by MAC in place of the serial number seen in the folder name of the micro image Note that the image file format is BMP e When a scale is displayed on the screen the scale is also added to the saved image However the scale cannot be displayed when the image size is 2560 x 1920 For the relationship between image size and the size of image files refer
20. Load Images Exceptions For the originating web site only Enable Java Enable Javascript Advanced Fonts amp Colors 116 2 VIII Networking Functions Install the Java plug in If a Java plug in to run Java applets is not installed you will be requested to install a Java plug in the first time you access ROOM2 Follow the displayed installation instructions e Click the image display area COOLSCOPE web controller v5 10 Mozilla Firefox SE File Edit View Go Bookmarks Tools Help ak E d amp O A O hpg2168102sjahtm G L Customize Links _ Free Hotmail Windows Marketplace Windows Media Windows Jk Additional plugins are required to display all the media on this page Install Missing Plugins x Interval Timer 200msec v Micro Size 320 240 Nikon reload COoOLSCOPE I WB Click here to download plugin e een ouaity Fe e Click Next Plugin Finder Service Available Plugin Downloads The Following plugins are available Java Runtime Environment Some plugins may require additional information From you during installation Press Next to install these plugins 117 VIII Networking Functions e The J2SE installation screen is displayed Select Typical setup and click Accept iin J25E Runtime Environmen
21. Service for Mac OS 10 2 Adding a user 1 Click User for MacOS 10 1 or Account for MacOS 10 2 in the system environment settings window 2 Click New user in the User window for MacOS 10 1 or the Account window for MacOS 10 2 and set the User ID and Password established in the External Server of the COOLSCOPE 3 Close the window 4 Select Apple Logo System Environment Settings Network Settings Built in Ethernet to check the server IP address On home directory settings and the separation of save directories for each of multiple users COOLSCOPEs When a user is added a home directory is automatically created under the specified user name and directory name at a location below Macintosh HD Users When image files are transferred the FTP Dir specified on the COOLSCOPE side is created at a position below this home directory and the image files are saved in that directory Example If the user name is CS and the FOLDER specified on the COOLSCOPE side is F then the FTP transfer directory is C Users CS F When accounts are added separate directories are created for each user so that even if there are multiple users COOLSCOPEs connected to the network image files are saved in each separate directory 125 VIII Networking Functions The access right for the created folder is set that only user can read and write and other users can only read To prevent
22. Specify the installation directory and choose which program Features to install You can change your choice of Features after installation by using the Add Remove Programs utility in the Control Panel Recommended for advanced users 114 VIII Networking Functions e Click Finish i Java 2 Runtime Environment SE v1 4 2_11 Complete InstallShield Wizard Completed The InstallShield Wizard has successfully installed Java 2 Runtime Environment SE v1 4 2_11 Click Finish to exit the wizard Cancel e If a message requests that you restart your computer close any open programs and click Yes to restart 3 Disable personal firewall blocking For details refer to Section 3 3 5 ActiveX Settings 115 VIII Networking Functions For Windows and Mozilla Firefox 1 Change the browser settings e Select Tools gt Options Mozilla Firefox File Edit View Go Bookmarks MME Help lt a X gt v e A 1 Web Search Ctrl K gt C Read Mail 0 new _ Customize Links _ Free Hotmail New Message Ctrl M Downloads Ctrl J Extensions Themes JavaScript Console Page Info Clear Private Data Ctrl Shift Del Options e On the Content tab select Enable Java e Select Enable JavaScript Options i Aena 2 General Privacy Content Tabs Downloads Advanced Block Popup Windows Allowed Sites Warn me when web sites try to install extensions or themes Exceptions
23. a A A E 41 1 1 Enlarging the macro image quitting enlargement s sssssssssrssrsnrsnrrnrrnrrnrennns 41 1 2 Switching between Multi screen and Full screen ssssssssnnsnnnnnnnnnnnnnnnnnnnnnnnnnn 42 1 3 Closing tHe CONURONCE ssaa a a aa a 42 1 4 Opening closing the help screen ssssssssnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn 43 1 5 Displaying NIGING the Scale rrn EN E 43 LO Changing Lne SCale COOLS rsssdccrsnss gash inin ARE ai 43 1 7 Displaying hiding focusing lines ON the macro IMAGE cccseeeeeeeeeeeeeeeeeeeaaaaaas 43 1 8 Changing the Colors Of TOCUSING lines irois a EAA 44 1 9 Displaying the AF area on the micro iMage sssssssssssssssrssrsnnsnrnnrenrrnrrnrrnrennns 44 2 AdJusting Mages an a a A a a 44 2 1 ZOOMING oUt ZOOMING IN ansa a E nit E E E Ea ea ae 44 2 2 ClOSiIng OPENING the apertUressnsisncgi stana ted a a tal E E ERA 44 3 DISPIAVINGNIMAGES ruis iare aa ad a aa vend a a a aa 45 3 1 Displaying images stored in the MediUM ssssssssssssrssrssrsnrrnrrnrrnrrnrenrrnrennns 45 3 2 Displaying the freeze frame iMage sssssssnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn 45 A Savilkg ODServaciOh CONGIGIONS cise seesteet aea a E AE Oia ta ie 46 4 1 Enabling the saving of observation conditions to the MediuM cccceeeeeeeeees 46 4 2 Saving the current observation conditions ss ssssssssnnsnnnnnnnnnnnnnnnnnnnnnnnnnnnnn 46 4 3 Replicating a set of Saved observation CON
24. an image with a frame e CMP 50 to 150 Specify the magnification for the real 10 print mode PictBridge standard The PictBridge standard was established for the communication between digital cameras and printers With this standard an image in a digital camera can be printed out directly with a printer without any PC For details refer to the official site of the PictBridge http www cipa jp pictbridge SAVE button Saves the settings Default button Resets the settings to their defaults LCD Chart button Adjust settings on the monitor using the chart incorporated in the COOLSCOPE Follow the procedure described below to make this adjustment A Press the LCD Chart at the lower right corner of the screen A monochrome mesh chart with one dot pitch will be displayed B Adjust the clock phase and clock pitch of the monitor Refer to the instruction manual provided with your monitor for detailed information on performing adjustments Some monitors have an automatic adjustment function C Once you have finished adjusting the display click on the lt lt BACK button 2 Ja VIII Networking Functions Networking Functions a Outline You can use a PC browser e g Internet Explorer Netscape Navigator to remote control the COOLSCOPE from your PC or to load images into your PC a2 Connecting Equipment Turn OFF the COOLSCOPE and the PC and consult your network administrator before connecting them
25. be transferred to a specified FTP server NOTE This function cannot be used unless an FTP server facility is available on the PC side Because up to five FTP servers can be specified images can be easily and quickly filed in a personal directory created separately one for each login user Furthermore when this function is combined with image file base software a more efficient image filing system may be created In this case the COOLSCOPE s FTP client function is used zgi s VIII Networking Functions 3 2 Network Settings 3 2 1 Items Requiring Confirmation Confirmation of network environments A LAN in some COOLSCOPE operating environments may have been set up in a way exclusive to that environment so be sure to consult your network administrator before setting up a PC This is necessary to avoid causing problems with the LAN Ask your network administrator to confirm the following points e Whether FTP can be used in the LAN only if you wish to use the FTP function e Whether image files can be flowed in the LAN e How IP addresses are managed fixed or automatic acquisition by DHCP If automatic acquisition of IP addresses etc is desired check whether a DHCP server is available In automatic IP address acquisition by DHCP the assigned IP addresses dynamically change Accordingly when using a WEB browser to access the COOLSCOPE from a PC we recommend setting a fixed IP address for the COOLSCOPE e Fixed IP
26. burnt out Contact the distributor The end response was not sent back to a control command The microscope s board is unable to communicate Turn on the COOLSCOPE again If the problem persists after restart contact the distributor Turn on the COOLSCOPE again If the problem persists after restart contact the distributor XI Troubleshooting 4 2 CF card FTP server and printer errors Message No CF card No saved data Media Full Media Error Data not saved on FTP server Illegal recall information file Illegal recall information file Description No CF card was found The NIKON folder is not found The medium is full and cannot Save any more data The medium is bad No CF card was found Data cannot be saved to the FTP server Cannot print Recall information is invalid and cannot be recalled The recall file is invalid and cannot be recalled 151 Remedy Check whether the CF card is in the COOLSCOPE If the problem persists with the CF card in the COOLSCOPE turn on the COOLSCOPE again If the problem persists after restart contact the distributor Image files captured using the COOLSCOPE are managed by the NIKON folder Deleting the folder or moving files from this folder makes it impossible to retrieve files Provide more available space in the medium Turn on the COOLSCOPE again if the problem persists If the problem persists after restart co
27. connected to a PC the disk drive for the CF card etc and the internal image drive of the COOLSCOPE are each recognized as one removable disk by the PC When the COOLSCOPE is connected to a PC two removable disks are displayed in My Computer on your PC s desktop File Edit View Favorites Tools Help Back gt QSearch GyFolders C4 R GS X A Ea Address J my Computer ka Go ESEI Floppy 4 JLocal Disk Cs i JLocal Disk D My Computer Local Disk E JLocal Disk F Removable Disk I 2 Compact Disc G Removable Disk Removable Disk H Capacity 60 8 MB SP User2 on Psgserv N S Psadata on Psgserv T Used 22 6 MB Control Panel C Free 38 2 MB 1 object s selected g My Computer Z The drive names of the removable disks differ with each PC environment In the following cases there might be a conflict between another connected device and the drive letter e A medium is inserted correctly into position but the message Please insert media is displayed when you click the icon for the media drive e A similar message is displayed when you click the icon for the CCD image drive e Connecting the COOLSCOPE immediately disables other USB device connected to the PC In such a case change the drive letter assigned to the media drive or the CCD image drive The following shows how to make this change 1 Select Start Control Panel Administrativ
28. from the ensuing menu The updated date and time will be displayed DS LZIMG File Edit wiew Favorites Tools Help Back Gisearch Folders GA Las OF x wey Ez Address DS L2IMG Open DS L2IMG Open With call WinZip IMG1260 Send T p JFEG Image eas uk Copy Modified 6 15 2005 7 07 PM Size 263 KB Create Shortcut Attributes normal Delete Rename Properties 136 VIII Networking Functions Removing the COOLSCOPE from the PC Because the COOLSCOPE is connected via a USB interface it can be removed from the PC even if the PC remains powered on However to prevent data from being corrupted by removing the COOLSCOPE during write operation follow the procedure described below 1 Eject the medium from the media drive before removing the COOLSCOPE 2 Unplug the USB cable or turn off the power to the COOLSCOPE 3 When the below is displayed click OK You have unplugged or ejected a device without stopping tt Unplugging or ejecting devices without first stopping them can often cause your computer to crash and lose valuable data To safely unplug or eject any of the following devices first use the Hardware wizard in the Control Panel to stop the device USB Mase Storage Device 4 Generic volume IF You frequently need to unplug this device Windows can give you an icon on the taskbar to quickly unplug or eject pour device If you would
29. image Adjust the white balance Move the observation position Change the magnification Bring the image into focus Adjust the image focus Electronic zoom Adjust the stop Adjust the image brightness Allow enabling disabling of the Extended Mode function Retrieve the micro image from the COOLSCOPE 99 VIII 12 TYPE list box 13 SIZE list box 14 QUALITY list box 15 Line list box 16 INTERVAL TIMER list box 17 Micro Size list box 18 ANNOTATION check box 19 Cursor list box Networking Functions Allow selection of an image file format at the time of micro image retrieval Allow selection of an image size at the time of micro image retrieval Allow selection of a JPEG compression ratio at the time of micro image retrieval Change the color of the cross line on the macro image Allow the selection of intervals for updating the micro image Select the size of the micro image Allow enabling disabling of the Annotation function Display the Annotation cursor 100 3 3 4 1 2 3 VIII Networking Functions Working with the WEB screen Updating the micro image and statuses ROOM 1 ROOM 2 ROOM 3 1 Click on the RELOAD button reload reload Adjusting the white balance ROOM 1 ROOM 2 ROOM 3 The white balance is adjustable 1 Click on an area of the macro image that does not show the specimen If the screen image is too
30. it darker White balance setting button WB Allows setting of the white balance The white balance cannot be set in the case of overexposure or underexposure Confirm that the green bar appears at the exposure level before initiating white balance setting lt White balance setting procedure gt 1 Click on the button to return to the Multi screen 2 Click on a specimen free area of the macro image At this time if the screen is too dark or if white areas appear at intervals use the AE button to make the necessary adjustments 3 Display the Details menu by clicking on the SETTING button 4 Display the CAM menu by clicking on the CAM tag 5 Display the Advn submenu by clicking on the Advn tab 6 Click on the WB button white balance setting button to set the white balance AE lock button AE_L The COOLSCOPE constantly adjusts its exposure to achieve the optimal image brightness Clicking on the AE_L button stops this adjustment thus fixing the screen brightness This button functions identically to the controller s AE_L button Either button can be used to enable or disable the AE_L Gain button GAIN Y R B Allows adjustment of the image brightness and color balance Change the Y level to adjust the image brightness which is not finely adjustable using the exposure correction button CMP Change the R and B levels to adjust the image color balance with reference to G Normally set them at 1
31. large media over 2 GB on your computer before use You cannot access the USB memory from the PC in a state where the USB memory is connected to the USB host port of the COOLSCOPE When you wish to read write from to the USB memory connect the USB memory directly to the USB port of the PC 1792 VIII Networking Functions USB interface boards and hubs The COOLSCOPE supports USB 2 0 If the COOLSCOPE and the USB interface board are connected in one of the following ways data is transferred at a rate of 12 Mbps compliant with the USB 1 1 standard USB 1 1 interface board USB 1 1 interface board USB 2 0 interface board COOLSCOPE USB 1 1 hub COOLSCOPE USB 1 1 hub COOLSCOPE If the COOLSCOPE and the USB interface board are connected in one of the following ways data is transferred at a rate of 480 Mbps compliant with the USB 2 0 standard USB 2 0 interface board USB 2 0 interface board COOLSCOPE USB 2 0 hub COOLSCOPE Make sure that the USB 1 1 interface board and USB 1 1 hub used in the system are compliant with the USB 1 1 standard and that the USB 2 0 interface board and USB 2 0 hub used in the system are compliant with the USB 2 0 standard To avoid unexpected problems we recommend that the interface board and the COOLSCOPE be connected in only one hub stage and not in cascaded hub stages x USB cables When multiple units of COOLSCOPE are connected to a Windows 2000 machine equipped with a USB 1
32. of the four drive letter buttons C D E and F on the menu which corresponds to the connected medium is changed from gray to white In the case of a CF card C is assigned to it without exception When a USB memory is connected either of D E and F is assigned to it Select a drive letter in the following manner A Put the cursor on a target drive letter and the periphery of the drive letter will be highlighted in yellow B Click on it with the mouse C The periphery of the selected drive letter is highlighted in red When a drive letter is selected the folder file name in the medium is displayed on the menu Reproduce button gt Reproduces the images saved in the medium The uppermost file in the file list at the lower half of the menu is reproduced Directly clicking on the name of a desired file at the lower half of the menu also reproduces its image without using this button Delete button RK Deletes the images saved in the medium The uppermost file in the file list at the lower half of the menu is deleted Thumbnail DISPLAY button Displays a thumbnail of the file stored in the specified directory among those images saved in the medium A thumbnail of the file saved in the same directory as that of the file at top of the list in the lower half of the menu is displayed If there are nine or more image files in a directory you can s
33. the COOLSCOPE ccceecce eee eeeeeeeeeeeeees 120 3 4 1 Using FTP Commands to Get COOLSCOPE Image Files from a PC 120 3 5 Using Telnet Commands to Operate on the COOLSCOPE c cece eens 121 3 6 Saving Images to an FTP Server from the COOLSCOPE cccceeeeeeeeeeeeeeees 122 3 6 1 Setting the FTP Server Functions of the PC cccccceeeeeeeeeeeeeeeeeeees 122 3 6 1 1 When Using Windows 2000 Professional or XP Professional 05 122 570 1 2 When Using Mac OS Xanadu ia a r ATA 125 3 6 2 Setting External Servers of the COOLSCOPE Network Settings 127 3 7 Using the COOLSCOPE when Connected to a PC with a USB Cable 129 3 7 1 Recommended Operating Environment sssssssssnsssnsnnnnnnnnnnnnnnnnnnnn 129 3 742 Connecting the COOLSCOPE and a PC resinicsseedesewsservntisavinndetaeinetasawine 131 Daves 3JBOPNVINGOWS lt 2 O00 Gtacted incase a neta Sead en cea eeades ones ce eaten aes 132 32774 FOF WINGOWS XP iieiaei ninae Derntabtatinaneudasamebtadaeennadcaamareataanes ta 138 3 7 5 Removing the COOLSCOPE from the PC ccc cece e cere eee eens 143 5 8 Using the Direct Print FUNCION asenada a dees a tee ees 144 52658 JPRINCOUL asco op cenat a eautea scons S aoe oece enemas neueauaees 144 3 8 2 Setting for the direct print fUNCtION 0 cece eee aa i idad 144 A Sind OPtonalACCeSSONeS jn cwesiiaciccnereeieeyeatiees tenses a 145 L ERGONOMIC CONUFO
34. wrench holder at the rear of the COOLSCOPE 1 2 lt Section B s Enlarged Section C The cover 1 on the right side is detached 3 Do not discard the packing case as it will need to be reused for future transportation Safety Precautions How to use this manual For first time users of the COOLSCOPE First read Using the COOLSCOPE This section will give you basic instructions on operating the instrument Vv Chapter IV Basic Observation Tasks This chapter describes basic observation tasks in sequence Chapter V Basic Image Capture Tasks This chapter describes basic image capture tasks in sequence Chapter VI More Sophisticated Tasks This chapter explains convenient functions not covered in the discussion of basic tasks Chapter VII Details Menu This chapter explains the COOLSCOPE camera setup and display setup Chapter VIII Networking Functions This chapter describes the procedure for connecting the COOLSCOPE to a network Please refer to this chapter if it is necessary to save images to the external FTP server or remotely control the COOLSCOPE from an external PC using a WEB browser Chapter IX Using Optional Accessories This chapter describes the procedure for connecting accessories to the COOLSCOPE and using them Chapter X Connecting Peripherals This chapter presents the patterns of connection between the COOLSCOPE and various peripherals 10 Safety Precautions Nota
35. 00 Gain levels below 100 will result in a reduced output saturation level Gains in excess of 100 may be set Tone curve control Draw a tone curve by dragging the curve The total characteristic of the tone curve will be calculated on the basis of the tone curve displayed in the tone curve control box Therefore to draw a curve from scratch select LINR as the base curve For details to save the curve data refer to 3 Registering the tone setting in Section 1 3 REG1 submenu Black level setting button SFT To reduce flares and noises in dark areas of the image or to sharpen the dark areas of the image specify a negative value 6i e 15 16 VII Details Menu Tone selector buttons TON1 to TON4 LINR TON6 and TON7 Seven options are available The contrast changes with each option Select a tone that suits your observation while checking it on the monitor TON1 Wide dynamic range Should be selected for a specimen with high contrast requiring shades of gray from dark to bright areas TON2 Low contrast Should be selected when low contrast is required Recommended specimen Cells tissues and general subjects TON3 Standard contrast Should be selected for normal image capture Recommended specimen Cells tissues and general subjects Ton4 High contrast Should be selected when high contrast is required This option provides a sharper image Recommended specimen Cells tissues and gene
36. B 1 6 Objective switchover error An unsuccessful attempt was made to move the COOLSCOPE Optical path switchover error An unsuccessful attempt was made to move the COOLSCOPE Magnification switchover error An unsuccessful attempt was made to move the COOLSCOPE Aperture stop drive error An unsuccessful attempt was made to move the COOLSCOPE Stage XY axes drive error An unsuccessful attempt was made to move the COOLSCOPE Stage XY axes at the drive range limit An unsuccessful attempt was made to move the COOLSCOPE when it was at the drive range limit Stage Z axis drive error An unsuccessful attempt was made to move the COOLSCOPE 149 Turn on the COOLSCOPE again If the problem persists after restart contact the distributor Turn on the COOLSCOPE again If the problem persists after restart contact the distributor Turn on the COOLSCOPE again If the problem persists after restart contact the distributor Turn on the COOLSCOPE again If the problem persists after restart contact the distributor Turn on the COOLSCOPE again If the problem persists after restart contact the distributor The COOLSCOPE cannot be moved further Reverse the direction of motion Turn on the COOLSCOPE again If the problem persists after restart contact the distributor XI Troubleshooting line1 Focus limit line2 Error Code nZMA7 nNZMB7 Macro light control error Error
37. CF card is inserted the message Insert Media is displayed with the click of a media drive icon The same message is displayed with the click of a CCD image drive icon Other connected devices get unusable The COOLSCOPE is recognized as a device such as the camera instead of a storage when connected to the PC A printer compatible with PictBridge cannot be used e PRINTER cannot be selected in SAVE TO e The error message PRINTER ERROR is displayed during printing XI Troubleshooting DE E S The warning HI SPEED USB Device Plugged into non HI SPEED USB Hub is displayed in the task tray system tray when connecting to the USB port based on the USB 1 1 Therefore it is not an error The COOLSCOPE functions as a device for USB 1 1 The conflict over the drive letters may have occurred between the COOLSCOPE and the connected other USB devices Change the drive letters referring to Outline of the Disk Drive in 3 7 3 For Windows 2000 in Chapter VIII The USB device operation method may have been set on Pict Bridge Set the operation method on Must storage function referring to 3 1 Power On Setting submenu in Chapter VII Check that the operation method of the USB device is set on Pict Bridge in Power On Settings of the SETUP menu Check that a printer compatible with PictBridge is connected to the USB D connector of the COOLSCOPE Check that the printer compatible with the COOLSCOP
38. CItIONS cece eeeeeeeeeeee eee eeeeeeeees 47 4 4 Erasing stored observation conditions ss ssssssssssssrsrrsnrsnrnnrnnnrnnrnnrnnrnrrnnene 47 4 5 Replicating a set of observation conditions saved in the MediuM ceeeeees 48 3 SCN G Up the Camera stints cab ranula dcaree des vad teeacielan ce sree ae daleabesdatees N adi aai 49 5 1 Calling up the camera setting suitable for the SpeECIMEN cccceeceeeeeee eee e eens 49 5 2 Setting the white DalanGes4 ccc oleae oavadces eavactes A eavaasen covaceeteats 50 5 3 Locking The automatic xPOSUre eikcevedsenievuiounisndie nies a A eedinnievntss 50 5 4 Displaying thumbpnailkimageS eisrean E ANE E ON 50 VIT Detalls Memnu risasi n a a a A A aN 51 IT CAM Menu Camera adJ stiMmeNntS Josienne ear aaa 53 Lok AQV i SUDMENU morrir n NE NENON 54 Le SAV 2 SUUDME NU ee rE A AE 59 LS REGL SUDMENU resene a a A ete ae ea 64 2 VIEW menu splitting the screen deleting stored iMaGES cece cece ee eee eee eee eees 66 3 SETUP menu network date and time and printer SettingS cccceeeeeeeeeeee eens 68 3 1 Power On Settings SUDMEN Ua sacdnisecicutas otadeniseande O EE ERa 69 3 2 NetWork Settings SUBMENU sais iristean e a eee RA a aiai 70 3 5 File Setings SUDMENU srias aaa aa a a a 75 3 4 Additional Settings SUBMENU sersa A AA Aa 76 Bike Contents VIII Networking FUNCHONSscriiravekconttadhecundatamnedaad eed caecadd asain datiiniidasdsadaeeenads 78 DP SQU
39. COOLSCOPE from a PC e Use a WEB browser e Use an FTP command e Use a Telnet command You also can store images on an FTP server from the COOLSCOPE via a network Possible operations in each method are described below The symbols lt q and p in the diagrams below indicate the flow of images while the symbol gt indicates the flow of control Using a WEB browser to operate the COOLSCOPE from a PC e Get images from the COOLSCOPE e Control the COOLSCOPE in a simple way A WEB browser may be used from a LAN based PC to observe images In this case the COOLSCOPE s HTTP server function is used NOTE As a rule although it depends on how frequently images are updated the COOLSCOPE can only be accessed from up to two PCs at the same time 80 VIII Networking Functions Using FTP commands to operate the COOLSCOPE from a PC e Get images from the COOLSCOPE e Send and save images to the COOLSCOPE e Advanced control of the COOLSCOPE cele B FTP commands may be used from a PC to receive send or save image files Furthermore advanced control of the COOLSCOPE is possible using the COOLSCOPE s FTP server function Using Telnet commands to operate the COOLSCOPE from a PC Telnet commands may be used from a PC to achieve advanced control of the COOLSCOPE In this case the COOLSCOPE s Telnet server function is used Operating on a PC from the COOLSCOPE e Send images to an FTP server Images can
40. CS1D_IMG folder and these files can be downloaded to the PC Because the CCD image drive is read only no image files can be sent from the PC to the COOLSCOPE The image files whose size is specified in the SETUP menu displayed after right clicking the SAVE button is stored in the CS1D_IMG Example IMG1280 bmp IMG640 bmp For detailed information on file settings refer to Section 2 Specifying the Image Storage Format Storage Medium File Name and Label Illumination in Chapter V The image files stored in the CS1D_IMG folder are updated once every 5 seconds Be aware that if files are read out at intervals shorter than 5 seconds you will not always get the latest image To confirm the time of day at which image files have been updated follow the procedure described below 135 VIII Networking Functions Confirming the updated date time of image files 1 2 Open My Computer and display image file icons Then select View Refresh to update the image file icons displayed DS LZIMG File Edit view Favorites Tools Help d Back Toolbars t ders g4 LS OS x wy FE v Status Bar Address E Explorer Bar d saat Large Icons Small Icons a List 1512780 Details Thumbnails IrtGi7s50 JPEG Image Arrange Icons 5 Modified 8 Line Up Icons Size 263 Kl Customize This Folder Attributes Go To Refresh Right click any image file icon of interest and select Properties
41. CUINIG tines ens ganeen send peace i toners E sane Pena en nee aaa ee aad Oe ee aes 78 CONNECUAG EQUIPIMGNE 2 i ccecesiecacenciede ee eteieeasaedeenieie ois deere ede a sae 78 2 1 Connecting the COOLSCOPE directly to the PC ccc ccc eee eee e eee e eens 78 2 2 Connecting the COOLSCOPE to a netWworK s sssssssssnnsnnsnnnnnnnnnnnnnnnnnnnnnnnnnnnn 79 3 Using the COOLSCOPE Vid a NETWORK sscie ee esvenee ie aes esas eo eee ge aaa ena cabs E teases 80 Sal Typical MetNod OF USCxdevaisscceaceaniauatensieateunise canarias lanes aie nienwee ates ramets 80 S22 NEIWORK Setn eccisusccscieunnecnneunseetieunaeanieun een EE 82 321 Items Requiring Confirmation saisssscacistieeaniersiesie Sasie Pe Setesie Sosasi ees 82 3 2 2 Setting Essential Items Network Settings cccccccccceeeee cece eee e eens 84 3 3 Operating the COOLSCOPE from a WEB BroOWSel ccceeeeeeeeeeeeeeeeeeeeeeeeeeeeees 88 Jsi Setting the WEB BrOWSER desasxicee sede ydin vedeuasain vein sede a a 88 3 3 2 Displaying the WEB SCreen iva scaidcncs ceaiadawnnnd caus Soriataa cael nets anad otaaaiadaess ces 90 55 0 WEB SClEGM UNCON Sisnccsocecncsuncaretavecaetie E E a 93 3 3 4 Working with the WEB screen sssssssssnnsnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn 101 Soe ACLIVEX SOUUINGS vansccnetacstihs calienet detates nasi cars deat anntens ener RR 110 315 0 Java ADDIE SERING S missactechascnsesciinadeaues E ner a aneenaeen 112 3 4 Using FTP Commands to Operate
42. Code nEMP 1 7 Macro light error Error Code nMACBO Micro light control error Error Code nLMP 1 7 Micro light error Error Code nMICBO Communication error Error Code None Device control error Error Code Stage Z axis at the drive range limit An unsuccessful attempt was made to move the COOLSCOPE when was at the drive range limit Macro lighting control error An unsuccessful attempt was made to turn on the lighting Macro lighting error The lighting was found to be OFF as a result of a status readout immediately after the lighting had been turned on Micro lighting control error An unsuccessful attempt was made to turn on the lighting Micro lighting error The lighting was found to be OFF as a result of status readout immediately after the lighting had been turned on The end response was not sent back to a control command The microscope s board is unable to communicate Control command execution errors other than the above An unsuccessful attempt was made to execute a control command other than the above 150 The COOLSCOPE cannot be moved further Reverse the direction of motion Turn on the COOLSCOPE again If the problem persists after restart contact the distributor The lamp is burnt out Contact the distributor Turn on the COOLSCOPE again If the problem persists after restart contact the distributor The lamp is
43. D H C P is enabled or disabled or the IP address is changed select SAVE shut down the COOLSCOPE and then restart it 1 Allows you to move among submenus 2 Return to Window button Returns you to the CAM menu 3 Enable NetWork Submenu buttons NetWork Settings Additional Settings Enables selection of whether the network functions are to be used To use the network functions check this box so that an asterisk appears Enables the network functions Disables the network functions 4 Read Only FTP WEB disable Write Control Allows you to write enable or write disable the Web and FTP server TO Operate the COOLSCOPE from another PC through the Web screen as when magnifications are changed uncheck this box so that the asterisk disappears Note however that this option may be left write disabled if you simply wish to retrieve images Write COOLSCOPE operation disabled _ Write COOLSCOPE operation enabled 70 VII Details Menu 5 Internal Server Specify the COOLSCOPE s IP address subnet mask user ID and password Refer to P 76 Settings for improved security for details on how to set a password D H C P Allows specification of whether or not an IP address should be automatically obtained by the D H C P server If an IP address is to be obtained automatically the D H C P server must exist on the network To access the COOLSCOPE from another
44. E is turned on If the print icon cannot be clicked even when the printer is turned on close the menu and turn on the printer again and then display the menu The printer cannot run normally due to a cause such as lack of papers or ink Return the printer to normal condition referring the manual of the printer in use and restart the print operation in the menu 156 XII Service and Maintenance Service and Maintenance BPW cleaning the COOLSCOPE To avoid discoloration and fading of printed characters do not use any organic solvents e g alcohol ethanol thinner on painted plastic and printed areas Use of a silicon cloth is recommended To clean the display and other peripherals please refer to their respective instruction manuals Cleaning the Surface of the Glass on the Tray Bottom Dirty surface of the glass on the tray bottom will degrade the image quality 1 2 3 4 Press the green switch specimen slide tray IN OUT switch at the front of the COOLSCOPE or click on the EJECT button Bg on the screen to open the tray Turn off the main power by setting the main power switch at the rear of the COOLSCOPE to the O position then detach the power cord plug from the outlet Clean with a soft clean cotton cloth lens tissue or gauze dampened slightly with absolute alcohol ethyl or methyl alcohol e Wear rubber gloves and use tweezers to avoid biohazard contamination and injury caused by a
45. ICY seser an ceded toner detraneedesnenseses tas edad sasendstreneecether seas tages 145 x Connecting Peripheral Snoad a a a aa 147 1 CONNECTING Peripherals siciccerect eles ntedertdeweded asked ORON 147 A a Contents XI Troubleshooting 2 ese seseseees eect eeeeeeeete nese eeeteeeteeeeeesseeeeeesseesseseseesteeeees 149 1 Error MESSAGES chica tuaice il tandweadown aba Adin alanis suaaveee en eNNadeNauuiie tain ae wesunane madenduadeeds 149 bl DVE CREORS 02220586 2 iccsd S novetone evade w atone teas ee ssaoss sue mesaneetnamersaeee es 149 1 2 CF card FTP server and printer Crrors ccccccccssccceeceeeeeuceueenteuceutenteuueuneens 151 Handling RrODIGINS sarreran aa dat wesentehetand tet tezese Medias dalneceadedstest teleesesehide 152 3 NEWWOrK PEODIGIINS srun aa aa ewan sono aia tues benevas cle vaceuaaeeenee une eee 153 XI Service and Maintenance s seeeseesseeceseeeeeeeeeeeeeeeseeeeseeesseeeteeeeteeeeeees 157 1 Cleaning the COOUSCOPE icc nei eiwtsinchcawhininci sree bie eens eh ee ee ee 157 2 Cleaning the Surface of the Glass on the Tray Bottom cece cece ee eee ee eeeeeeeeees 157 3 Cleaning tnesOD CCU Cec agen ets sonar ceca tease dare ee eas ete ce ciate ees eens tees 158 4 Decontaminating the MicrOScOpe cccc cece eee e cee e ee aa aaa 160 5 Removing the specimen slide if it is accidentally left in the COOLSCOPE 160 Tansportng Camping arenen a rods a edi aeiouis pene nds m
46. M307E 07 5 NF711 2 M307EN07 Nikon COOLSCOPE II lt User s Manual gt There are two COOLSCOPE models available reflecting the difference in magnification specifications standard and wide ranged The COOLSCOPE User s Manual is based on the standard model If your model is the wide ranged model CS1DW please read the following information in addition to the rest of the manual y About Magnification Usable magnifications differ between the standard and wide ranged models as shown below Table Product Specification and Magnifications Product Specifications Magnification Standard 40X Wide ranged Since only magnifications for the standard model CS1D appear in the user s manual for the CS1DW 5X and 10X should be replaced with 2X and 4X respectively the other magnifications 20X and 40X apply to both models Example magnification is 5X should read magnification is 2X Note Rear view Nameplate ae ren e gt e The model name can be found on the nameplate on the rear of your COOLSCOPE Thi wit Rul eration is subijec Q he following two condi Model i 1 is device i 2 Name s device com Me h Part 15 of the es i aes ee Y Enlarged Mikon EA View e The current magnification is shown on the multi screen Magnification v Caution on using 2X and 4X magnifica
47. TP Site specify the home directory or default home directory to which image files are to be transferred Also select the Write check box to enable writing to the home directory When image files are transferred the FOLDER specified on the COOLSCOPE side is created at a location below this home directory and the image files are saved in that directory Example If the home directory is C CSFTP and the FOLDER specified on the COOLSCOPE side is F then the FTP transfer directory is Co CSFIP E 7 NOTE e The contents described above can also be set form Control Panel Administrative Tools Internet Information Services e Please refer to 2 Specifying the Image Storage Format Storage Medium File Name and Label Illumination in Chapter V for information on how to select a folder Other settings When enabling logon as an anonymous user In order to discriminate the user by which image files are written to the FTP server we in the above settings adopted a method to log in with a user ID and a password However it is possible to log in as an anonymous user 1 When setting External Server in Network Settings of the COOLSCOPE select anonymous for the User ID and none for the Password 2 Enable the FTP server PC for anonymous access As in home directory settings select the Allow Anonymous Logon check box on the Security Account tab in the Preset FTP Sites properties
48. W and SETUP You can move from one menu to another by clicking on the tags at the top of each menu e Clicking on the button at the upper right corner of each menu returns you to the Multi screen e Clicking on the iO button at the upper right corner of each menu resizes the menu ee Click on the SETTING button A 1 2 3 VII Details Menu CAM menu camera Adv2 adjustment REG1 meterin g area shading correctio n tone custom mode Clicking on VIEW menu the e g splitting the screen upper right deleting stored corner of each images menu returns you to the Full ae eee aT ae re Te ae RONEN Shee enn AS een ee eee nae Te eee eee ee eee ee Settings SETUP menu 2 e g setting up network time and printer CAM menu The CAM menu can be used to adjust the camera and contains two submenus Adv1 Advanced menu 1 and Adv2 Advanced menu 2 Clicking on the tab approximately 2 3 of the way up the menu allows you to move among submenus Changes made to the settings are reflected in the images until the COOLSCOPE is shut down Shutting down the COOLSCOPE returns all settings to their defaults Up to seven sets of settings can be saved and readily called up later For more information refer to P 53 VIEW menu The VIEW menu can be used to perform tasks such as splitting the screen into two halves live and freeze frame images and deleting images in the medium This men
49. ad COOLSCOPE I WE xtende Pick Up Image DownLoad Quality Fine Line 98 VIII Networking Functions The ROOM 3 screen features the following functions Observing images can be displayed at a predetermined time interval and captured to the COOLSCOPE And the COOLSCOPE body can be controlled Micro image Macro image Controller 1 RELOAD button 2 WB button e ia WE Interval Timer 5sec W K ta 17 RUN button KJ pe paa 3 MOVE button ty 16 Interval Timer list box Magnification r MAG i o button 5x tox 20x 40x 4 5 AF button Focus ABEE 6 FOCUS button as 7 DIGITAL ZOOM button PTEE 8 APERTURE button AE 9 AE button C Extended Mode 10 Extended Mode Pick Up Image DownLoad check box Middle 640x480 x Quality Fine Type Size 11 DOWNLOAD button 12 TYPE list box 13 SIZE list box 14 QUALITY list box Annotation Cursor None Line 15 Line list box 19 Cursor list box 18 ANNOTATION check box Names and functions of buttons and list boxes 1 RELOAD button 2 WB button 3 MOVE button 4 MAG button 5 AF button 6 FOCUS button 7 DIGITAL ZOOM button 8 APERTURE button 9 AE button 10 Extended Mode check box 11 DOWNLOAD button Update the micro
50. address only when use of it is allowed When a fixed IP address is acquired a subnet mask and a gateway address are also decided Note however that when you apply for the acquisition of an IP address you may be requested to supply the MAC address of your product The MAC address is shown at the lower right corner of the Network Settings screen of the SETUP menu lt 82 VIII Networking Functions Confirmation of the PC that is connected to a network e OS and browser versions Applicable OSs and Web browsers depend on the URL to be connected Therefore select a suitable URL for your PC OS and Web browser Besides there may be a slight difference in operation depending on the OS and browser versions Recommended OS and browser Room1 Windows 2000 SP4 Example http 192 168 10 2 Windows XP SP2 Internet Explorer 6 02 SP2 Room2 Windows 2000 SP4 Example http 192 168 10 2 csja htm Windows XP SP2 Internet Explorer 6 02 SP2 Firefox 1 5 0 4 Room3 Windows 2000 SP4 Windows XP SP2 Internet Explorer 6 02 SP2 Firefox 1 5 0 4 Lunascape 3 3 5 Opera 9 0 Netscape 8 1 Mac OS X Ver 10 3 or later Firefox 1 5 0 4 Opera 9 0 Safari 1 2 Example http 192 168 10 2 cs htm The URL addresses in the above table are examples of the case when the IP address of the COOLSCOPE is set as 192 168 10 2 Use your COOLSCOPE address in place Refer to 3 2 2 Setting Essential Items Network Settings
51. addresses and time information of the machine that has accessed the COOLSCOPE can be recorded For the access from the WEB browser access log can be remained only when the user ID is set in the Internal Server of the COOLSCOPE But the access log is cleared after turning off the COOLSCOPE When executing the Telnet w_NLGON command default setting is changed and the NETLOG can be displayed To load the log data access to the COOLSCOPE by FTP command and load NETLOG txt in the RAMIBOX directory The size of the NETLOG txt is 64kbyte or less When the buffer gets full the file is automatically cleared to O byte And then writing starts However when the setting that the file is forwarded to an external server by the w_NLFTON command is established the LOG date is forwarded to the external server when the file is cleared The forwarded file is named as NL month date time txt ex April 1st 12 00am is named NLO40112 txt Telnet command related to the log w_NLGON indicate the NETLOG w_NLGOF hide the NETLOG S NLGCL clear the contents in the NETLOG w_NLFTON forward the NERLOG when the buffer is full w_NLFTOF not forward the NERLOG when the buffer is full 872 VIII Networking Functions Operating the COOLSCOPE from a WEB Browser 3 3 1 Setting the WEB Browser 1 Start the PC Refer to your computer s user manual for details 2 Launch Internet Explorer Refer to your computer s user manual or the Internet Explorer he
52. ain color density hue black level and contrast adjustments noise suppression sharpness color effect save various settings as custom mode delete settings and confirm image capture and focus conditions e Adv2 submenu Of the settable Advi submenu items this submenu contains those most frequently used exposure level shutter speed metering area and mode exposure correction white balance AE locking camera gain black level contrast and focus condition Besides the image brightness histogram can be displayed for the fine adjustment of the exposure e REG1 submenu menu subordinate to the Advi and Adv2 submenus This submenu enables registration of metering area shading tone and custom mode cl to c7 settings 4 Clicking on the button at the upper right corner of each menu returns you to the Multi screen Eu Oe VII Details Menu aa Advi submenu 1 2 3 i x lt CMP GAIN PALI Menu tags CAM VIEW SETUP Allows you to move among menus Resize button D Changes the menu size Close button 1 Menu tags 2 Resize button 3 Close button 4 Exposure level 5 Submenu tabs 6 Shutter speed 7 Metering area selector button 8 Metering mode selector button 9 Exposure correction button 10 White balance setting button 11 AE lock button 12 Gain button 13 Density button 14 Hue button 15 Black level setting button 16 Tone selector
53. an access your COOLSCOPE via a network NOTE Note that if you save a password as the default word none without changing it the word none will become your password Restriction of IP access To restrict the IP address that can be accessed from the COOLSCOPE select the IP Authority checkbox And register the IP address allowed to access according to the following procedure Registration method of the IP address allowed to access 1 Set the CF card or USB memory on the PC NOTE A card adapter may be required 2 Creat the NET directory in the CF card or USB memory 3 Creat a text file on which the IP addresses allowed to access are indicated Example indication 192 168 10 1 192 168 10 10 192 168 10 36 192 168 10 21 A wildcard is available 1 to 254 4 Supply the extension jp to the text file and save it under the NET directory ex Autho jp 5 Insert the CF card or USB memory into the COOLSCOPE 6 Access the COOLSCOPE from an external PC via Telnet and execute the w_IPA command to register the IP addresses Refer to the instructions in this chapter 3 5 Operation by Telnet command Insert a CF card or a USB memory before executing the w_IPA command Then NET directory of the inserted CF card or USB memory is searched and the IP address of the searched text file with extension jp is registered 86 VIII Networking Functions Save of user s log files NETLOG function The IP
54. aneously and display the ROOM 1 screen a same image will appear on all PCs The refresh time intervals for all PCs are the same too The last settings are used for all browsers on all PCs NOTE If the Web screen displays the following image the COOLSCOPE network setting is set to Read Only Refer to 3 2 NetWork Settings submenu in Chapter VII Interval Timer MAX Micro Size 320 240 v Nikon ad COOLSCOPE I ME xtende Pick Up Image DownLoad Type bmp Size Middle 640x480 v Quality Fine Line 95 VIII Networking Functions The ROOM 2 screen features the following functions Observing images can be displayed at a predetermined time interval and captured to the COOLSCOPE Stream images can be displayed And the COOLSCOPE body can be controlled To use the ROOM 2 screen the Java applet must be set up Refer to 3 3 6 Java Applet Settings When the ROOM 2 is used for the first time the following security warning dialog box may appear Warning Security The application s digital signature has been verified Do you want to run the application Name L 4pplet Publisher Mikon Corporation httosi 128 31 30 92 Cancel More Information ij The digital signature has been validated by a trusted source This program is provided with a digital signature for certification Therefore check the Always trust content from this publisher check box
55. appear automatically 4 Select the name of the folder in which the desired set of observation conditions is Saved Reading of stored observation conditions starts Click the CLOSE button to close the Recall folder list and halt the reading operation 5 Click on the NUMBER button with a number corresponding to the set of observation conditions to be replicated The set of observation conditions for the clicked NUMBER button is replicated In the absence of a specimen slide in the COOLSCOPE the image saved in the medium is replicated 48 VI More Sophisticated Tasks 5 Setting up the Camera ea Calling up the camera setting suitable for the specimen Camera settings scene modes HE PAP AZ suitable for HE papanicolaou and azan stained specimens can be called up Camera settings custom modes cl c2 c3 adjusted using the CAM menu can also be called up For more information on the CAM menu please refer to VII Details menu 1 Right click on the WB button The button changes as follows each time it is right clicked WB gt HE gt PAP gt AZ gt C gt C2 gt c3 The camera settings in the table Description of Camera Settings shown below are assigned to the HE PAP AZ c1 c2 and c3 buttons respectively Display the desired camera setting button 2 Click on the camera setting button displayed in Step 1 The camera setting assigned to the selected button is called up Caution Cli
56. at the rear of the COOLSCOPE to the position to turn ON the main power 159 XII Service and Maintenance 4 Decontaminating the Microscope Normally use 70 medical alcohol for decontamination If the specimen is spilled check whether it is hazardous If so handle the spill according to the standard procedure used by the laboratory Use of organic solvent may discolor plastic components Removing the specimen slide if it is accidentally left in the COOLSCOPE If the specimen slide is accidentally left in the COOLSCOPE due for example to malfunction follow the steps described below to remove it 1 Set the main power switch at the rear of the COOLSCOPE to the O position to turn OFF the main power Cover Hexagonal head bolts 2 Detach the left and right side covers To do so loosen the two hexagonal head bolts on each side at the rear of the COOLSCOPE and slide the covers backward 3 Check the position of the specimen slide left inside the COOLSCOPE from the left side window If the objective is located toward the front push it in to the right 4 If the specimen slide holder is located more toward the front than the window slide the specimen slide toward the front and push it out As the door begins to open completely remove the specimen slide while holding the door by hand Wear rubber gloves to avoid biohazard contamination and injury 5 If the specimen slide holder is located in front of the window sl
57. b 15 pin cable USB H terminal Connect the USB mouse or other USB device to this terminal AN Caution Do not insert a USB memory USB D terminal The terminal is used to connect with a PC for data transmission or a PictBridge compatible printer for direct printing 10 LAN terminal This terminal is designed for network connection Connect this terminal to the LAN terminal of an external PC or hub with a network cable 10BASE T 100BASE TX USB memory connector Connect a USB memory here Do not insert or remove the USB memory when data transfer AN Caution This port is used for a USB memory only Do not connect other USB device such as a USB mouse 11 CF card slot Insert the CF card into this slot Press the button at the left edge of the slot to take out the card Never attempt to insert or take out the card during a data write operation while the LED indicator is lit 16 AUX IN terminal Connect an external input device such as the ergonomic controller D ERG C Contents Connecting the COOLSCOPE and Peripherals Lee eae 1 2 3 4 4 Required equipment COOLSCOPE Power cord CF card Monitor analog RGB input Use a PC monitor capable of TTL synchronization at SXGA 60 Hz USB mouse Use a Nikon recommended product Making connections Connect the display or projector to the COOLSCOPE s MONITOR terminal Wind one turn of the mouse cable on the ferrite core Connect the mouse to the COOLSCOPE
58. basic settings are described here Refer to your OS s help function and other materials for more detailed information on settings 3 6 1 1 When Using Windows 2000 Professional or XP Professional The following explains how to set FTP functions in Windows 2000 Professional or XP Professional The Control Panel is displayed in a different way on the two operating systems e In Windows 2000 you select Start Settings Control Panel e In Windows XP you select Start Control Panel Other than that almost all setting operations are common to both Setup procedure 1 Install Internet Information Service IIS Add FTP server functions 2 Add a user User ID and Password input Set the COOLSCOPE as the login user 3 Specify the home directory Specify the home directory when logged in 4 Enable writing to the server Enable writing of image files to the FTP server S172 VIII Networking Functions Adding components If Internet Information Service IIS does not have components added you need to install them 1 Open Control Panel and select Add Remove Programs Add Remove Windows Components 2 Select Internet Information Service IIS and click the Advanced button 3 Examine whether the FTP File Transfer Protocol Services check box is selected If not select it and click OK After that follow the installation instructions You will be requested for a Windows 2000 or XP CD ROM dur
59. brasion powder from the slide glass and broken pieces of the glass e Follow the manufacturer s directions for use when using absolute alcohol e Replacement is recommended if the glass is heavily stained or damaged For replacement contact the distributor Insert the power cord plug into the outlet Then set the main power switch at the rear of the COOLSCOPE to the position to turn on the main power e The tray is retracted initializing the COOLSCOPE 157 XII Service and Maintenance 30 Cleaning the objective 1 The power to the COOLSCOPE must be completely shut off before the objective is cleaned POWER e Right click on the POWER OFF button 22 on screen to shut down the COOLSCOPE e Always remove the specimen slide 2 Set the main power switch at the rear of the COOLSCOPE to the O position to turn OFF the main power and remove the power cord plug from the receptacle 3 Detach the left and right side covers To do so loosen the two hexagonal head bolts on each side at the rear of the COOLSCOPE and slide the covers backward i j Cover Hexagonal head bolts 4 Detach the right hand objective e Todo so first loosen the securing ring from the right side window in the direction indicated by the arrow If the ring is difficult to rotate slip the supplied hex wrench into the hole on the securing ring and turn the ring e Turn the objective in the direction indicated by the arr
60. bring the image into focus display a high contrast area of the image at the center of the field of view and then click on the AF button again Note that right clicking on the AF button enables confirmation of the AF area For more information please refer to 1 9 Displaying the AF area on the micro image in Chapter VI e During auto focusing a progress bar is displayed on the micro image to indicate the process status When the bar reaches the end auto focus is complete e The color of the progress bar will be the same as the color selected for the scale Zoom Progress bar ___ ae G 2 o ei C FO03 1MAGEOOO BMP 31 0CT 10 10 cc IV Basic Observation Tasks 4 Observing the Specimen on the Slide 4a Moving the observation position You can move the observation position in any of three ways as follows Clicking somewhere on the macro image 1 2 Click somewhere on the macro image A micro image of the clicked position is displayed You can continuously move the observation position by dragging while holding down the left mouse button NOTE Right clicking activates the auto focus function after the observation position shifts to the selected position Clicking somewhere on the micro image Click somewhere on the micro image The micro image moves to center on the clicked position You can continuously move the observation position by holding down the left mouse button Clickin
61. button 16 Histogram display 17 Focus indicator 1 Menu tags CAM VIEW SETUP Allow you to move among menus 2 Resize button iO Changes the menu size 3 Close button Closes the menu and returns you to the Multi screen 50 4 5 6 7 8 VII Details Menu Exposure level Lev Shows for guidance purposes whether the current exposure level is appropriate Light blue bar Underexposure Green bar Appropriate exposure Red bar Overexposure If the light blue or red bar appears in the absence of exposure correction the COOLSCOPE is unable to control its exposure Therefore change the image brightness using the exposure correction button CMP so that the green bar appears Submenu tabs Adv1 Adv2 Allow you to move among submenus Shutter speed The correct shutter speed setting is displayed Metering area selector button Narrow o Slightly narrow E Wide You can select which part of the image is to be metered for determination of the exposure level Normally select Wide Select Narrow to avoid effects from the surrounding areas of the screen You can set the size and the position of the area freely on a1 to a3 Displaying and setting photometry area The photometry range is displayed with a red frame for a limited time when the size of the photometry area is changed The red frame is kept displayed when the icon is right clicked with the mouse When
62. buttons 17 Noise suppression buttons 18 Sharpness buttons 19 Effect buttons 20 Clear settings button 21 Custom mode buttons 22 Scene mode buttons 23 Info box 24 Focus indicator Closes the menu and returns you to the Multi screen sba 4 5 6 7 8 VII Details Menu Exposure level Lev Shows for guidance purposes whether the current exposure level is appropriate Light blue bar Underexposure Green bar Appropriate exposure Red bar Overexposure If the light blue or red bar appears in the absence of exposure correction the COOLSCOPE is unable to control its exposure Therefore change the image brightness using the exposure correction button CMP so that the green bar appears Submenu tabs Advi and Adv2 Allow you to move among submenus Shutter speed The correct shutter speed setting is displayed Metering area selector button Narrow o Slightly narrow E Wide You can select which part of the image is to be metered for determination of the exposure level Normally select Wide Select Narrow to avoid effects from the surrounding areas of the screen You can set the size and the position of the area freely on a1 to a3 Displaying and setting photometry area The photometry range is displayed with a red frame for a limited time when the size of the photometry area is changed The red frame is kept displayed when the icon is right clicked with the mouse When
63. cking on the button while WB appears on it starts white balance setting For more information please refer to 5 2 Setting the white balance Description of Camera Settings Button Setting beseipton Scene Setting suitable for HE stained specimens modes PAP Papanicolaou stain Setting suitable for papanicolaou stained specimens Setting suitable for azan stained specimens Custom cl Custom 1 Values obtained through camera adjustments using modes the CAM menu c2 Custom 1 Values obtained through camera adjustments using the CAM menu c3 Custom 1 Values obtained through camera adjustments using the CAM menu NOTE e The scene mode settings cannot be changed e Up to seven combinations c1 to c7 of values obtained through camera adjustments can be saved using the CAM menu Note that only custom modes c1 through c3 can be called up from the Multi screen To call up any of c4 through c7 click on the desired custom mode button in the CAM menu 49 VI More Sophisticated Tasks 5 2 Setting the white balance The white balance is factory set but can be changed as desired Follow the steps described below to change the white balance as necessary 1 Click on a specimen free part of the macro image lle At this time if the screen is too dark or if white areas appear at intervals use the AE button to make the necessary adjustments 2 Click on the WB button The white balance is adjusted
64. commended settings click Default Level Custom Level Default Level iJ Activex controls and plug ins ij Automatic prompting For Activex controls Disable Enable J Binary and script behaviors Administrator approved Disable Enable i Download signed ActiveX controls Disable Enable Prompt J Download unsigned Activex controls GX _r Li s Reset custom settings Reset to Medium e Check the Enable option for Run ActiveX controls and plug ins e Check the Enable option for Script ActiveX controls marked safe for scripting 110 VIII Networking Functions Welcome Microsoft Internet Explorer File Edit view Favorites Tools Help Q Back gt x a A P Search 5 2 Favorites 4 R 4 ee 3 Address http 128 31 30 36 Internet Options General Security Privacy Content Connections Programs Advanced K Select a Web content zone to specify its security settings amp 0O Internet Local intranet Trusted sites Restricted sites Internet 4 This zone contains all Web sites you haven t placed in other zones Security level for this zone Custom Custom settings To change the settings click Custom Level To use the recommended settings click Default Level Security Settings Settings J Run ActiveX controls and plug ins Administrator approved Disable Enable
65. copic image Macro image displaced at the center of the macro image area Unable to turn off the power shut down the COOLSCOPE All on screen buttons are disabled The COOLSCOPE starts up when the main power is turned on without switching on the power switch ee T e Is the power cord properly connected e Is an appropriate power cord used e Is the side cover closed e Is the main power switch at the rear turned on set to the position e Are all cables properly connected e Are all cables connected to appropriate terminals e Is the display turned on No mouse cursor appears e Is the mouse properly connected to the appropriate on screen terminal e Is the slide glass of the correct size for the specimen slide used e Is the specimen slide chipped on the corners or scratched e Is the specimen slide held down with the holder e Is the image properly in focus e Is the cover glass of the proper thickness for the specimen Slide used e Is the specimen slide s cover glass facing up e Is the specimen slide dirty e Is the COOLSCOPE exposed to much vibration during use e Place the specimen slide properly on the tray Bring the specimen slide in contact with the right side of the tray frame and fix it in position using the specimen slide holder e Have you clicked on the on screen POWER OFF button e Is a specimen slide placed on the specimen slide tray e Is there any dust or other foreign partic
66. d aperture level can be selected from among the following options 25 50 75 100 Standard level 75 1 Click on the APERTURE button NOTE e Right click to enable adjustment in 5 increments range 25 to 100 sA a VI More Sophisticated Tasks 30 Displaying images 34 Displaying images stored in the Medium You can display images stored in the medium CF card or USB memory 1 2 3 20 Displaying the freeze frame image Click on the PLAY button One of the images stored in the medium CF card or USB memory is displayed Images are displayed in descending order from most to least recent each time the PLAY button is clicked Right click to display images in order from oldest to most recent The live image will appear on screen again upon completion of the display of all images on the medium CF card or USB memory The live image reappears when you click on the IMAGE button You can display the freeze frame image 1 2 Click on the IMAGE button m The freeze frame image appears The live image reappears when you click on the IMAGE button again NOTE When you switch from the live image to the freeze frame image or vice versa the IMAGE button changes as follows Live image m Freeze frame image LIVE AG VI More Sophisticated Tasks 4 Saving observation conditions The COOLSCOPE is capable of saving up to 12 sets of observation conditions and replicat
67. d rear when transporting it Recesses e Do not install the COOLSCOPE against the wall Leave space of at least 10 cm from the wall Be sure to also provide 10 cm of space on top of the COOLSCOPE e Do not install the COOLSCOPE in a locker or cabinet Safety Precautions AN ca ution 5 Be careful of the sliding tray in the front of the device e A tray extends approximately 10 cm from the front of the COOLSCOPE e Position the COOLSCOPE properly on the table making sure that there are no obstacles in terms of both personnel and equipment within an area approximately 10 cm from the front of the COOLSCOPE e Do not place your hand or any object in the area within approximately 10 cm from the front of the COOLSCOPE e Do not look into the tray door e Do not hold the tray with your hand or push or pull it by force Tray 6 Caution against long continuous observation To relieve fatigue as a result of long observation limit your continuous observation time to one hour or less and take a 10 to 15 minute break between continuous observations Adjust the layout of other equipment such as the display and the mouse in accordance with the position of the COOLSCOPE and the height of your chair 7 Disposal of the COOLSCOPE To avoid biohazard risk dispose the COOLSCOPE as contaminated equipment according to the standard procedure of your laboratory Safety Precautions Handling the COOLSCOPE 1 Handle the COOLSCOPE car
68. dark or too white adjust with the AE button 2 Press the WB button to perform white balance adjustment W E NOTE Changing the white balance automatically resets the camera gain to Y100 R100 and B100 Changing the observation position The observation position can be changed in any of the following three ways Clicking on the macro image ROOM 1 ROOM 2 ROOM 3 1 Click on the macro image The micro image appears at the clicked position Clicking on the micro image Only for ROOM 3 This function does not work on the ROOM 1 and 2 1 Click on the micro image The micro image appears at the clicked position Clicking on MOVE button ROOM 1 ROOM 2 ROOM 3 1 Click on the MOVE button The observation position moves by half the micro image screen per click sejal NOTE The observation position cannot be continually moved from the PC by pressing and holding the mouse button In addition the observation position cannot be coarsely adjusted by right clicking on MOVE button Note that the micro image on the ROOM 3 is updated when the observation position is changed with the PC This function is disabled if the COOLSCOPE network setting is set to Read Only 101 VIII Networking Functions Caution Position information information on the position clicked on the macro or micro image on the PC is calculated with the upper left corner of the image on screen as the origin and is transmitt
69. de out of the tray EEI shutting down the CooLscoPE AN Caution Always take the specimen slide out of the COOLSCOPE before shutting it down 1 Click on the POWER OFF button on screen A confirmation message Ready to shut down appears MENEE S em 2 75 i u Amm C F003 1MAGEO0D BNP E POWER OFF button 2 Click on the OK button The COOLSCOPE is turned off NOTE If you do not intend to use the COOLSCOPE for extended periods set the main power switch to the O position to turn off the main power 24 III Description of the Multi Screen Description of the Multi Screen a Screen Layout Micro image Controller PAIA 0a EEN TUAN I toa nA if eN K RAE AE oe g Sr a Nikon Far eee pS ode TS P f i aar 55 STER n COOLSCOPE I r as J 42 A de Somes 165 f A a Ari Ip i te p SMS gt be OE A amp O gt eee y BoU e amp goug t re 7 e Le fe 7 SSA a Sh aN axe er Magnification 7 o REE b Ghee A 3 ee K i Digital on gt Eo Ez a 7i E x i Po a a i a d y ae 4 VA i pi Pe i 7 g p A j d f 2 v Y D j z 4 _ d 1 lt ey e gt el x id eae gt 3 E anin ne a Ae T UNS aad S AAT id x TT z ths gt s s 7 A z 7 z G 5 z gr en EuN 3 SIT H 2 A CCPC ce di a
70. disables network operation D H C P Auto get an IP Internal Server ip address 128 031 030 177_ Sets the IP address of SubNet Hask 255 255 000 000 ere User ID anonymous max 10 char Pass Word none max 10 char Enable FIP Client IP Authority External Server ip address 192 168 020 001 ping test Server No 1 User ID anonymous max 10 char Pass Word none max 10 char gateway 000 000 000 000 Mogin test Save Default MAC Address 00 90 B5 42 00 01 skip 0 1 2 3 4 5 6 ji g a TE e e Ban Ere Ea l m w E E E ea Eae Enable Network To use a network always select this check box Read Only FTP WEB disable Write Control This is to disable writing to the internal files of the COOLSCOPE when it is operated using a WEB browser or FIP command When this check box is selected you cannot write to the internal files If you only want to get images from the COOLSCOPE leave this check box selected 84 VIII Networking Functions Internal Server Set the IP address subnet mask user ID and password of the COOLSCOPE Refer to Settings for improved security described below for details on how to set a password e When automatically acquiring an IP address D H C P Auto get an IP IP Address Automatically set after restarting Subnet Mask Automatically set after restarting NOTE The COOLSCOPE
71. e PC to other media files in the same way as files are normally manipulated on a PC Removing media When the COOLSCOPE is connected to a PC follow the procedure described below to remove the media from the media drive 1 Check to see that the PC card indicator on the COOLSCOPE is turned off Close the menus displayed on the COOLSCOPE 2 If any image files have been loaded from the media into the PC close all open image files on the PC 3 Right click the media drive removable disk icon on the My Computer screen and select Eject 134 VIII Networking Functions sioj xi File Edit view Favorites Tools Help da Bark mp Gh Search U4 Folders Ea Ss GS x A Ei Address My Computer 344 Floppy tA Local Disk 01 Local Disk Ds JLocal Disk Es Local Disk Fs Removable Disk 1 2 Compact Disc G Removable Disk Removable Disk Hi Open Capacity 60 8 MB SA User c ote E EP Psgdat 3 p i 22 earch Used 22 6 MB a Control ree 35 aring C Free 38 2 MB Shari Cal Winzip Format Create Shortcut Rename Properties Ejects the removable disk From the drive A 4 Remove the media manually from the COOLSCOPE Using the CCD Image Drive Receiving image files Double click the CCD image drive removable disk icon on the My Computer screen to access the internal CS1D_IMG folder of the COOLSCOPE The latest image files shot by the COOLSCOPE are stored in the
72. e contact your nearest Nikon representative 120 VIII Networking Functions 35 Using Telnet Commands to Operate on the COOLSCOPE You can use Telnet commands to operate the COOLSCOPE from your PC NOTE Always be sure to consult your network administrator before executing Telnet commands Your institution s network may have been set up in a unique manner so invalid operations could cause a problem on your network Enter the IP address of the COOLSCOPE from the MS DOS prompt command prompt of Windows to connect to the COOLSCOPE Example Telnet 192 168 10 2 A detailed command list is available from Nikon If you wish to have one please contact your nearest Nikon representative 121 VIII Networking Functions 3 6 Saving Images to an FTP Server from the COOLSCOPE In this instruction manual explanations are given assuming that the IP address of the COOLSCOPE is 192 168 10 2 that of the server is 192 168 10 1 and that of the subnet mask is 255 255 0 0 while the gateway address is 192 168 128 1 In practice you should use the IP address assigned to you by your network administrator It is assumed that network settings on the PC side LAN connection have been completed before this function can be used Please consult your network administrator 3 6 1 Setting the FTP Server Functions of the PC For image files to be transferred from the COOLSCOPE you need a PC which has FTP server functions Only
73. e Tools Computer Management Disk Management 2 Right click the volume whose drive letter you want to change and select Change the drive letter and path 3 Click Edit and choose one of unassigned drive letters from the select box or enter a drive letter directly from the keyboard and click OK 4 Click OK in the ensuing confirmation dialog box 135 VIII Networking Functions How to identify the media drive and the CCD image drive Right click any removable disk icon on the My Computer screen to display its Properties dialog box This dialog box helps you identify the drive assigned to that removable disk In the case of the CCD image drive for example it can be identified based on the volume label NIKONIMG and its capacity 16 0 MB as shown below Removable Disk H Properties 7 x General Tools Hardware Sharing Label NIKONIMG Type Removable Disk File system FAT Hi Used space 366 640 bytes 360 KE o Free space 16 410 624 bytes 15 6 MB Capacity 16 7 79 264 bytes 16 0 MB cancel soot i Note that because this drive is write protected its volume label cannot be altered Using the Media Drive Sending receiving image files Insert media into the card slot of the COOLSCOPE and double click the media drive removable disk icon on the My Computer screen to access the media The image files on the media drive can be retrieved from the media or transferred from th
74. e eased eee tetas 25 2 gt Names and FUNCTIONS On BULLONS siradan biter diate euelae E 26 IV__ Basic Observation Tasks s serrrerrnerrrerrrrerererenerenerererrrrerererererererrrerrrer ST 1 Placing a Specimen Slide In the COOLS COPE vssesvesccseccsccsnessnesceeacesearuseeewesteiwaceews 32 2 Selecting an ODSernvation POSION Asesinas eee eee ee 33 3 Bringing the Image into Focus AUtO FOCUSING cccccee cece eect e eee ee ee eeeeeeeeeeeaneeees 33 4 Observing the Specimen on the Slide ccccce eee A NA ERAT NE aSa 34 4 1 Moving the observation Position cc cece cece c A A AAAG 34 4 2 Changing magnifications so224 4620 ooice dete oe nce aoe neh eee ek 34 4 3 Adjusting the image TOCUS lt 2c22 cos seca eked sctes cose a A hei enaes cosietes nese aees cont 35 4 4 Darkening brightening the image sssssassnsnsnnnnnnnnnnnnnnnnnnnnnnunnnnnnnnnnnnnnnnnnnnn 35 5 Taking the Specimen Slide out of the COOLSCOPE cccceecceeeeceeeeteeteeeeeeseunenes 35 V Basic Image Capture Tasks sssscerteantes it sunsnsecinasasee iraa aeei 36 i Setting a Medium to the COOES COPE raseona n TE AE 36 2 Specifying the Image Storage Format Storage Medium File Name ahd kabel MgmiNatiO Ny ea ran E EEN a coe cae an AN E E EEEN 38 2 Capturing an Mage ssenari O EA AAE ON NN 40 12 Contents VI More Sophisticated TaSkS ssssrrerrrerrrrrerrrnerrrerrrrrrrrerererrrrererererrrerrrrrrrr AL t Dishayind The SCOS oerien
75. e mouse cable on the ferrite core 3 Connect the mouse to the COOLSCOPE s USB H terminal 4 Connect the power cord to the COOLSCOPE s AC inlet USB H terminal MONITOR terminal AC inlet 4 Insert the CF card into the CF card slot at the front of the COOLSCOPE CF Card slot 20 II Starting up and the Shutting down the COOLSCOPE Starting up and the Shutting down the COOLSCOPE a Turning the Power on off Set the main power switch at the rear of the COOLSCOPE to the position to turn on the main power A s gt O k p jo Main power switch to the position Normally leave the main power on If you do not intend to use the COOLSCOPE for extended periods shut down the COOLSCOPE following the normal procedures Then turn off the main power by setting the main power switch to the O position sJj II Starting up and the Shutting down the COOLSCOPE EPE Startup 1 Press the power switch on the right side of the COOLSCOPE Press the power switch The title screen appears on the display Nikon COOLSCOPE I Logo appearing on screen The multi screen is displayed when the COOLSCOPE is initialized s99 II Starting up and the Shutting down the COOLSCOPE 3 Inserting and Removing a Specimen Slide 1 Open the tray to insert a specimen slide You can open the tray in one of two ways as follows e Click on the
76. e output for the COOLSCOPE is in analog RGB format and is transmitted via a D sub 15 pin connector e USB mouse e Network cable 10 100BASE cross cable Connection to USB printer e COOLSCOPE and power cord e Display SXGA analog RGB input 60 Hz TTL sync e Cable to connect the COOLSCOPE to the display Image output for the COOLSCOPE is in analog RGB format and is transmitted via a D sub 15 pin connector e USB mouse e USB printer compatible with PictBridge e USB hub Connection to USB keyboard e COOLSCOPE and power cord e Display SXGA analog RGB input 60 Hz TTL sync e Cable to connect the COOLSCOPE to the display Image output for the COOLSCOPE is in analog RGB format and is transmitted via a D sub 15 pin connector e USB mouse e USB keyboard e USB hub 148 XI Troubleshooting Troubleshooting a Error Messages An error message may be displayed in the event of malfunction Drive errors line1 Objective switchover error line2 Error Code nLZC 1 7 Optical path error Error Code nPDM 1 7 Mag switchover error Error Code nOBJ 1 7 Aperture stop error Error Code nASC 1 7 line1 Position shift error line2 Error Code nXYA 1 6 nxYB 1 6 nULD 1 6 nX1P 1 6 nX2P 1 6 nXCP 1 6 line1 End of movable range line2 Error Code nXYA7 NXYB7 nNULD7 nX1P7 NX2P7 nXCP7 line1 Focusing error line2 Error Code nZMA 1 6 nZM
77. e screen mode between the Multi screen and the Full screen Push here to lock the automatic exposure While locking the automatic exposure the LED of this switch lights up Rotate this knob to focus on the specimen Rotate this knob to move the view point right and left on the screen Rotate this knob to move the view point up and down on the screen LED A This LED lights up when the power is ON LED B This LED blinks during the white balance adjustment 146 X Connecting Peripherals Connecting Peripherals This chapter explains the minimum requirements for connection between the COOLSCOPE and peripherals Connecting Peripherals Connection to projector e COOLSCOPE and power cord e Projector SXGA analog RGB input 60 Hz TTL sync e Cable to connect the COOLSCOPE to the display Image output for the COOLSCOPE is in analog RGB format and is transmitted via a D sub 15 pin connector e USB mouse Direct connection to PC or server e COOLSCOPE and power cord e Display SXGA analog RGB input 60 Hz TTL sync e Cable to connect the COOLSCOPE to the display Image output for the COOLSCOPE is in analog RGB format and is transmitted via a D sub 15 pin connector e USB mouse e Network cable 10 100BASE cross cable i47 X Connecting Peripherals Connection to network e COOLSCOPE and power cord e Display SXGA analog RGB input 60 Hz TTL sync e Cable to connect the COOLSCOPE to the display Imag
78. e specimen positioned at the center of the field of view e If it is difficult to bring the image into focus display a high contrast area of the image at the center of the field of view and then click on the AF button again Executing auto focus from the PC automatically updates the micro image e This function is disabled if the COOLSCOPE network setting is set to Read Only Adjusting the image focus ROOM 1 ROOM 2 ROOM 3 Click h OCUS b i 1 Click on the FOCUS button Focus AFE are NOTE Adjusting the image focus from the PC automatically updates the micro image This function is disabled if the COOLSCOPE network setting is set to Read Only Using electronic zoom ROOM 1 ROOM 2 ROOM 3 Electronic zoom can be used Five options 100 140 200 280 400 and 800 are available to choose from as the zoom factor 1 Click on the DIGITAL ZOOM button Daan Efi NOTE The selected zoom factor is not reflected in the COOLSCOPE s micro image 103 8 9 VIII Networking Functions Adjusting the aperture ROOM 1 ROOM 2 ROOM 3 The aperture can be adjusted Four options 25 50 75 and 100 are available to choose from for the aperture standard 75 1 Click on the APERTURE button aperture rex NOTE Adjusting the aperture from the PC automatically updates the micro image This function is disabled if the COOLSCOPE network setting is set to R
79. ead Only Adjusting the image brightness ROOM 1 ROOM 2 ROOM 3 A desired image brightness level can be selected Thirteen 13 options 2 0 1 6 1 3 1 0 0 6 0 3 0 0 3 0 6 1 0 1 3 1 6 and 2 0 are available to choose from for the image brightness level 1 Click on the AE button a I Ea NOTE Adjusting the image brightness automatically updates the micro image This function is disabled if the COOLSCOPE network setting is set to Read Only 104 VIII Networking Functions 10 Enabling disabling the Extended Mode function ROOM 1 ROOM 2 ROOM 3 If Extended Mode function is enabled certain button functions on the WEB screen controller will change as described in the table below 1 Click the Extended Mode check box to enable the Extended Mode function Extended Mode 2 Uncheck the Extended Mode check box to disable the Extended Mode function en Function enabled Function disabled MOVE button The observation position moves The observation position moves by one micro image screen per by half the micro image screen Click per click MAG button AF executes after the Magnifications are changed magnification is changed This is valid only when magnification is changed from low to high FOCUS button Manual focus coarse Manual focus fine adjustment adjustment The amount of adjustment per click is about 10 times the amount of adjustment achieved by fine focus adj
80. ed for special effects e BlueB Blue back image The dark level of NEGA is colored in blue This effect is intended for special effects e Sepia Sepia tinted image This effect is intended for special effects 20 Clear settings button CLSet Clears all settings back to their defaults 21 Custom mode buttons c1 to c7 Save combinations of settings custom modes so that they can be easily called up later Up to seven custom modes can be saved The REG1 submenu under the Adv1 Submenu can be used to save custom modes 22 Scene mode buttons HE PAP AZ Camera settings suitable for three types of stain methods are assigned to these buttons HE HE stain PAP Papanicolaou stain AZ Azan stain 23 Info box Text information saved along with the custom mode during custom mode saving is displayed here 24 Focus indicator Shows the subject s focusing status both in bar and numeric form When adjusting the focus search for a position that provides the largest number of bars and the highest focusing number 58 VII Details Menu Adv2 submenu 1 Menu tags 2 Resize button 3 Close button 4 Exposure level 5 Submenu tabs 6 Shutter speed 7 Metering area selector 8 Metering mode selector 9 Exposure correction button 10 White balance setting button 11 AE lock button 12 Gain buttons 14 Black level setting button 13 Tone curve control 15 Tone selector
81. ed setting all press SAVE button to save what you ve set The server with the number you last saved is selected when the COOLSCOPE starts up Connection test Ping test To test the connection state of the external server press a ping test button on the Network Settings screen to execute a ping test This function is used to inspect whether the specified server exists in the LAN This connection test is performed on the server that is shown in the IP Address column of External Server However it is necessary aS a premise that the IP Address has been saved by pressing SAVE button When a response is received from the server a message OK Complete is displayed Ping may not always be responded to for reasons of security functions such as packet filter firewall or security gateway Ping test may be reexecuted after disabling the security function of the server In that case restart the COOLSCOPE first Also even when Ping was not responded to there is no problem with the server connection only if the server can be logged in in the login test described below Login test To test logging in to the server press a login test button on the Network Settings screen to execute a login test A login test is performed on the server that is shown in the IP Address column of External Server by using its set User ID and Password However it is necessary as a premise that the User ID and Password have been saved by pressing SAVE bu
82. ed to the COOLSCOPE As a result if the observation position is changed on your PC by clicking on the image with the upper left corner of the macro or micro image not shown on screen the clicked position will differ from the updated image center For instance if you click on e on your PC when the macro image is displayed on your PC as shown below a double boxed area will appear with x at its center Remember that in this case the area enclosed by a dotted line does not appear with the clicked e position at its center Image displayed on the PC The shaded area of the COOLSCOPE macro image is hidden 4 Changing magnifications ROOM 1 ROOM 2 ROOM 3 1 Click on the MAG button Magnification Changing magnifications automatically adjusts the lighting brightness and aperture 5x 10x 20x 40x NOTE e Changing magnifications from the PC automatically updates the micro image This function is disabled if the COOLSCOPE network setting is set to Read Only 102 5 6 7 VIII Networking Functions Bringing the image into focus ROOM 1 ROOM 2 ROOM 3 The image is brought into focus with 5x selected as the magnification 1 Click on the AF button The image is automatically brought into focus Focus AF NOTE When the AF button is used for the first time following specimen slide replacement always select 5 x as the magnification Note also that the AF button must be used with th
83. eee eee aes 161 A SLOFAGC wilt acca ceaniaa tesa E eucisn cia crate bapetectarctele anid hes toes eat E pales wats el ateleen ines E tele aides 163 XII Specifications and Confirming StandardsS ccccceecesee cesses eeeeeeeeeeeeeaneees 164 L SPCCHICATIONS skate indintine catdwelaeia is satin nati AoA hated ola isedd anreite embed bedhead ai ans 164 SP Gris Using the COOLSCOPE Name and Function of Each COOLSCOPE Part l 8 USB H terminal 12 USB memory connector 10 LAN terminal 13 AUX IN terminal 4 POWER indicator 6 Specimen slide tray entrance exit 5 Tray OPEN CLOSE switch 2 AC inlet 3 Power switch 1 Main power switch Main power E Set the switch to the position to turn on the power Normally leave the power on AC ACinet Connect the supplied power cord here Power switch Press this switch to turn on the power You cannot turn off the power with this switch Please refer to Chapter II section 4 Shutting down the COOLSCOPE for instructions on turning off the power a POWER indicator This indicator is lit when the power is on Ba Tray OPEN CLOSE switch The tray opens and closes alternately each time the switch is pressed Specimen slide tray entrance exit The specimen slide tray pops out and retracts through this opening opening The door opens and closes automatically AN Caution Do not look into this opening MONITOR terminal Connect a display to this terminal with a D su
84. efully The COOLSCOPE is a precision instrument Avoid shocks and handle it carefully When transporting it by car clamp its sections For more information please refer to Chapter XII Service and Maintenance 2 Weak electromagnetic waves The COOLSCOPE emits weak electromagnetic waves Do not bring precision electronic devices close to it as this may degrade their performance If you find that your TV or radio reception is affected move the TV or radio slightly away from the COOLSCOPE 3 Dirt on the tray s bottom glass and the objective Dirt on the glass at the bottom of the tray or the objective will result in poor image quality Clean the glass according to the instructions in Chapter XII Service and Maintenance 4 Removing the specimen slide if it is accidentally left in the COOLSCOPE If the specimen slide is accidentally left in the COOLSCOPE due for example to malfunction remove the specimen slide in accordance with the instructions given in Chapter XII Service and Maintenance 5 Installation location Since the COOLSCOPE is a precision instrument its use or storage in an inappropriate environment may result in malfunction or degraded performance Take the following into consideration when selecting an installation location e Select a vibration free location and install the instrument on a level surface e Avoid locations exposed to direct sunlight or immediately under room light and bright locations e
85. ent from rising excessively above the cover glass top surface as this may stain or damage the objective BAD GOOD SSS oo CO o o 2 AN WE EE PE V U 7 Unpacking and Unclamping 1 Check the contents to ensure that the package contains the following e COOLSCOPE e Hex wrench e Ferrite core attached to the mouse cable e User s manual e CF card 2 Unclamp the movable sections of the power cord before connecting the cord The power cord is clamped at three locations A B and C Section A Remove the cap from the right hand side and turn the two internal bolts counterclockwise with the supplied hex wrench until they are completely loosened These bolts will not come off even when fully loosened Reattach the cap to its original position once the bolts are loosened Safety Precautions Section A Sections B and C Detach the cover 1 on the right side To do this loosen the two thumbscrews 2 at the rear of the COOLSCOPE and slide the cover backward Turn the shiny metallic bolts in sections B and section C one bolt in each counterclockwise until they become loose the bolts will not come off even when fully loosened Keep the removed bolts in a safe location along with the hex wrench as these will be reused for future transportation of the COOLSCOPE Reattach the cover If the cover is not properly reattached a safety mechanism will prevent the COOLSCOPE from functioning Place the hex wrench in the hex
86. ep in mind that transferred images and information are not encrypted NOTE If none is left during password entry none will be your password J1 VII Details Menu 6 Enable FTP Client Enable specification of whether or not the FTP client function is to be used data is sent to the FTP server Check this box so that an asterisk appears when the FTP client function is to be used FTP client function enabled FTP client function disabled 7 External Server Specify the server PC s IP address and gateway Server No Up to five servers can be saved Assign a number to each server IP Address Enter the server PC s IP address here User ID Password To improve security a user ID and password can be specified for limited access to the server PC up to 10 characters If a user ID is specified authentication is requested at the time of connection to the browser It is not possible to log in unless the user ID and password are entered If the user ID remains anonymous anyone can access the COOLSCOPE Please keep in mind that transferred images and information are not encrypted NOTE If none is left during password entry none will be your password GateWay Sets the gateway address Example of External Server settings Server No Up to five servers can be saved Assign a number to each server IP Address lt Example User ID Enter a user ID for login to the serve
87. es can be downloaded to the PC A Because the CCD image drive is read only no image files can be sent from the PC to the COOLSCOPE The image files whose size is specified in the SETUP menu displayed after right clicking the SAVE button is stored in the CS1D_IMG Alternatively if the settings are updated on the REC menu the updated size will be valid Example IMG1280 bmp IMG640 bmp For detailed information on file settings refer to Section 2 Specifying the Image Storage Format Storage Medium File Name and Label Illumination in Chapter V The image files stored in the CS1D_IMG folder are updated once every 5 seconds Be aware that if files are read out at intervals shorter than 5 seconds you will not always get the latest image To confirm the time of day at which image files have been updated follow the procedure described below Confirming the updated date time of image files 1 Open My Computer and display image file icons Then select View Refresh to update the image file icons displayed DS L2IMG File Edit AVES Favorites Tools Help Toolbars Back Status Bar Search Key Folders Fil Explorer Bar Address Filmstrip Thumbnails a s Picti Tiles F IMalz80 fl View amp cons List 5 Grdi h Details ky Print ff a Arrange Icons by Choose Details 0 To Refresh File and Make a new Folder Ee Publish this Folder ta the Web E3 Share this Folder Ot
88. ex wrench Reattach the caps in their original positions 161 XII Service and Maintenance Section A Clamping sections B and C 1 Detach the right cover To do this loosen the two hexagon head bolts at the COOLSCOPE rear and slide the cover backward 2 With the supplied hex wrench screw one clamping bolt each fully into each of sections B and C clockwise 3 Reattach the cover to its original position and use the supplied hex wrench to secure it with the bolts at the rear 1 2 unu I il i Enlarged Section B Section C The cover 1 on the right side is detached 162 XII Service and Maintenance Storage If you do not intend to use the COOLSCOPE for an extended period turn off the main power by setting the main power switch to the O position then detach the power cord from the outlet Store the device in a dry location where mildew will not readily develop 163 XIII Specifications and Confirming Standards Specifications and Confirming Standards Specifications NIKON digital microscope COOLSCOPE Observable specimen Cover glass equipped specimen slide Thickness 0 9 to 1 7 mm slide glass specimen cover glass thickness not included e Slide glass Be sure to use a JIS R3703 1998 or ISO 8037 1 1986 compliant glass Thickness 0 9 to 1 2 mm Width 25 to 26 mm Length 75 to 76 mm Cover glass Be sure to use a JIS R3702 1996 or ISO 82551 1986 c
89. g on the MOVE button 4 2 Changing magnifications 1 Click on the MOVE button The observation position moves by a distance of half the micro image screen per click You can continuously move the observation position by pressing and holding the left mouse button NOTE By right clicking the observation position moves a distance of one micro image screen width per click Click on the MAG button When the magnification is changed lighting brightness and the aperture are automatically adjusted NOTE Right click first to change the optical zoom magnification then to perform auto focus This function is enabled only when the optical zoom magnification is adjusted from low to high magnification If the magnification is changed from high to low right clicking will not activate auto focus F a IV Basic Observation Tasks 43 Adjusting the image focus 1 Click on the FOCUS button The amount of adjustment per click varies depending on the selected magnification gt Pressing and holding the left mouse button changes the amount of adjustment continuously NOTE e A single right click results in coarse adjustment i e in increments approximately ten times larger when the left mouse button is subsequently clicked e As a guide during focusing the figure in the text box to the right of the FOCUS button changes in the 1000 range This figure is for guidance purposes only Even if the figure i
90. h the cursor arrow mark on the micro image of the PC The display color can be selected from None Red Yellow Green Blue Black or White The cursor display is updated at the same time of the update of the micro image Hone 1 Click the ANNOTATION checkbox to enable the ANNOTATION Gursor a function PADI eee 2 Click the Cursor list box to select one from the color table 3 Select None to disable the display function of the cursor Caution Position information information on the position clicked on the macro or micro image on the PC is calculated with the upper left corner of the image on screen as the origin and is transmitted to the COOLSCOPE As a result the observation position is changed on your PC by clicking on the image with the upper left corner of the macro or micro image not shown on the screen the clicked position will differ from that of the updated mouse pointer For instance if you click on e on your PC when the macro image is displayed as shown below the mouse pointer will appear at the x position Remember that in this case the mouse pointer does not appear at the clicked e position Image displayed on the PC The shaded area of the COOLSCOPE macro image is hidden 109 3 3 5 VIII Networking Functions ActiveX Settings The ActiveX controls must be enabled to use ROOM1 The settings can be performed only when the Internet Explore
91. he color of the cross line can be changed The display color can be selected from None Red Yellow Green Blue Black or White 1 Click the Line list box to select one from the color table 2 Select None to clear the cross line Selecting intervals for updating the micro image ROOM 1 ROOM 2 ROOM 3 The refresh time interval can be set here Select an option from the list The micro image is updated at the selected time intervals Note that the times of this list are guidelines and may slightly differ from the values 1 Click on the INTERVAL TIMER list box and select the desired intervals from the list Interval Timer Interval Timer O00 msec a dih aie a ROOM 3 ROOM 1 ROOM 2 107 17 18 19 VIII Networking Functions Selecting the resolution for the micro image ROOM 1 ROOM 2 Select the resolution of the image The greater the resolution is the slower the image is displayed 1280 960 is the slowest Select one for your convenience 320 240 Low resolution 1 16 pixel skipping 3204240 v Ww EEES iT 40 4a0 1260 960 Micra Size 640 480 Medium resolution 1 4 pixel skipping 1280 960 High resolution no pixel skipping Starting pausing updating of the micro image ROOM 3 1 ane RUN button to start micro image eee ee Run 2 Click on the RUN button again to pause updating
92. he magnification Bring the image into focus Adjust the image focus Electronic zoom Adjust the stop Adjust the image brightness Allow enabling disabling of the Extended Mode function Retrieve the micro image from the COOLSCOPE Allow selection of an image file format at the time of micro image retrieval Allow selection of an image size at the time of micro image retrieval a7 VIII Networking Functions 14 QUALITY list box Allow selection of a JPEG compression ratio at the time of micro image retrieval 15 Line list box Change the color of the cross line on the macro image 16 INTERVAL TIMER list box Allow the selection of intervals for updating the micro image 17 Micro Size list box Select the size of the micro image The ROOM 1 can refresh images faster than the ROOM 2 when the Windows 2000 SP4 or Windows XP SP2 OS is used Therefore if you can use either screen use the ROOM 1 screen Use the ROOM 2 only when you cannot use the ROOM 1 When multiple PCs access one COOLSCOPE simultaneously and display the ROOM 1 or 2 screen a same image will appear on all PCs The refresh time intervals for all PCs are the Same too The last settings are used for all browsers on all PCs NOTE If the Web screen displays the following image the COOLSCOPE network setting is set to Read Only Refer to 3 2 NetWork Settings submenu in Chapter VII Interval Timer Max w Micro Size Nikon relo
93. her Places x ee NIEONIMG 1s Ami n moa 141 VIII Networking Functions Right click any image file icon of interest and select Properties from the ensuing menu The updated date and time will be displayed f DS L2IMG File Edit View Favorites Tools Help pack j a PO search gt Folders Hab Address 1 405 L2IMG Picture Tasks r View as a slide show Preview Edit Print Order prints online jb Print this picture En Set as desktop background a Copy to cO Open With PC cillin Send To luk Copy File and Folder Tasks 9 Create Shortcut mij Rename this File Delete iy Move this File Rename Copy this File g Publish this file to the Web E mail this File Delete this file Properties 142 VIII Networking Functions 3 7 5 Removing the COOLSCOPE from the PC Because the COOLSCOPE is connected via a USB interface it can be removed from the PC even while the PC remains powered on However to prevent data from being corrupted while removing the COOLSCOPE during a write operation follow the procedure described below 1 Eject the media from the media drive before removing the COOLSCOPE 2 Click the Safely Remove Hardware icon on the task bar Safely remove USB Mass Storage Device Drivel Safely remove USB Mass Storage Device DrivelH al in begs cent ra I E ed Ea ut for 3 Drives should be selected one at a time First select the media dri
94. his message VECy CIE DIN A F a lows AP Professional valuation copy Build 2505 fi NIKONIMG 1 p gt Ba eer 1 41 PM 138 VIII Networking Functions Outline of the Disk Drive When the COOLSCOPE is connected to a PC the disk drive for the CF card etc and the internal image drive of the COOLSCOPE are each recognized as one removable disk by the PC A screen may be displayed prompting for confirmation of how you want the removable disk to be handled by the OS For the media drive this screen is displayed at the same time media is inserted into the media drive For the CCD image drive because it is labeled NIKONIMG this screen is displayed as shown below The drive name differs with each PC environment In this confirmation screen for either the CCD image drive or the media drive select the Open Folder to View Files Using Explorer check box and the Always Do the Selected Action check box Then click OK button Windows can perform the same action each time you insert S a disk of connect a device with this kind of file Pictures What do vou want Windows ta do using Fhoto Printing wizard be View a slideshow of the images using Windows Picture and Fas Viewer e Copy pictures to a folder on my computer using Scanner and Camera Wizard Open folder to view files using Windows Explorer 9 Take no action m When you click OK button the Explorer screen opens sh
95. iate berms of use and privacy policy You can remove these applications easily at any time 118 VIII Networking Functions e After a message is displayed indicating installation is complete click Finish i J2SE Runtime Environment 5 0 Update 9 Complete Installation Completed The Install Wizard has successfully installed J25E Runtime Environment 5 0 Update 9 Click Finish to exit the wizard Finish e Click Restart Firefox Now and restart the browser Software Update Firefox 1 5 0 6 Ready to Install Firefox has just completed downloading an important update and must now be restarted so that the update can be installed Update Firefox 1 5 0 8 Details Click Restart Firefox Mow to close all Firefox windows and install the update Click Later bo continue without restarting The update will be installed the next time you start Firefox Restart Firefox Mow 3 Disable personal firewall blocking For details refer to Section 3 3 5 ActiveX Settings 119 VIII Networking Functions 34 Using FTP Commands to Operate the COOLSCOPE You can run FTP commands on your PC to get images from the COOLSCOPE NOTE Always be sure to consult your network administrator before executing FTP commands Your institution s network may have been set up in a unique manner so invalid operations might cause a problem on your network 3 4 1 Using FTP Commands to Get COOLSCOPE Image Files from a PC
96. ide the specimen slide forward until it reaches a point at which it can be easily picked up and remove it using tweezers Wear rubber gloves to avoid biohazard contamination and injury 6 Reattach the covers at their initial positions 160 6 Transporting Clamping XII Service and Maintenance When transporting the COOLSCOPE by automobile follow the steps described below to clamp the mobile sections in place Three sections A B and C need to be clamped You must completely shut off the power to the COOLSCOPE before clamping 1 2 3 Click on the POWER OFF button on screen to terminate your tasks e Always remove the specimen slide Press the power switch on the right side of the COOLSCOPE Wait for roughly 10 seconds and set the main power switch at the COOLSCOPE rear to the O position to turn off the main power then detach the power cord plug from the outlet Caution Next time the main power is turned on the COOLSCOPE will start up without pressing the power switch on the right side Clamping section A 2 3 Detach the cap from the left side of the COOLSCOPE and fully turn the two internal bolts counterclockwise with the supplied hex wrench Left side Caution To prevent malfunction be sure to operate the work in 1 and then operate the work in 2 Detach the two caps from the right side of the COOLSCOPE and fully turn the two internal bolts clockwise with the supplied h
97. ing them In addition observation conditions can be saved to a medium CF card or USB memory when such saving is enabled Note that all observation conditions saved in the COOLSCOPE memory are erased when the specimen slide is removed Saving the current observation conditions onto the medium CF card or USB memory allows them to be reused when the same specimen slide is observed later Enabling the saving of observation conditions to the Medium When saving to the medium CF card or USB memory is enabled the current observation conditions can be saved to the medium CF card or USB memory and used when the same Specimen slide is observed later Note that the medium CF card or USB memory can replicate the desired observation conditions using the saved image even without the Specimen slide as the medium also saves the macro image and the micro images under individual observation conditions 1 Right click on the MEMORY button Ray The Settings screen is displayed Enable the saving of observation conditions to the media E no ves OK CANCEL Settings Screen 2 Check the YES checkbox to enable saving to the medium Check the NO checkbox to disable saving to the medium 3 Click on OK NOTE If you use the POWER OFF button to switch off the COOLSCOPE the observation save enable disable conditions are saved These settings are reloaded the next time power is switched on a2 Saving the current observation condit
98. ing this course If you are using a preinstalled version it should normally be copied to a location below the System directory on drive C Thus you only need to specify the directory that contains the required files in order to have them installed If the files cannot be found other folders will be searched Settings All of the following items can be set from Control Panel Administrative Tools Computer Management Adding a user Create a new user account 1 Select Computer Management Local System Tools Local Users and Groups 2 Right click on User to select New User 3 Enter a user ID and a password To ensure that the same information has been set for External Server in Network Settings of the COOLSCOPE enter the user ID and the password within ten alphanumeric characters in lower or uppercase Deselect User must change password at next login check box NOTE You also can create accounts and set passwords from Control Panel Users and Passwords or User Accounts for XP Specifying the home directory and enabling write to it Enable image files to be written from the COOLSCOPE to the PC by FTP 1 Select Computer Management Local Services and Applications Internet Information FTP Sites 2 Right click Preset FTP Sites and select the Home Directory tab in the ensuing Properties dialog box 123 VIII Networking Functions 3 In Local Path for Directory at F
99. ings a65 e VII Details Menu EPE VIEW menu splitting the screen deleting stored images The VIEW menu allows the screen to be split into two halves and enables images saved on the medium to be reproduced or deleted 1 Click on the SETTING button The Details menu is displayed 2 Display the VIEW menu by clicking on VIEW at the top of the menu 3 Click on the button at the upper right corner of the menu to return to the Multi screen 1 Menu tags 2 Resize button 3 Close button 4 Split screen button 5 Single screen button 6 Drive letter 7 Reproduce button 8 Delete button 9 Thumbnail DISPLAY button 10 File name list 1 Menu tags CAM VIEW SETUP Allows you to move among menus 2 Resize button D Changes the menu size 3 Close button Closes the menu and returns you to the Multi screen 66 4 5 6 7 8 9 VII Details Menu Split screen button DWIN Splits the screen into left and right halves Left half Freeze frame image at the moment of clicking Clicking on the Reproduce button displays the reproduction screen Right half Live image The freeze frame image on the left is updated each time this button is clicked Single screen button SWIN Restores the split screen to a single screen Drive letter selection button When a medium is connected to the COOLSCOPE one of the drive letters is assigned to the medium One
100. ions Follow the step described below to save the current observation conditions to the COOLSCOPE memory 1 Click on the MEMORY button v A number appears on the macro image At the same time the RECALL button with the same number is displayed in the Memory area Numbers are assigned automatically incremented by 1 NOTE When saving to the medium CF card or USB memory is enabled the observation conditions and the micro image are saved together on the medium 46 VI More Sophisticated Tasks 43 Replicating a set of saved observation conditions Follow the step described below to replicate a set of observation conditions saved in the COOLSCOPE memory 1 Click on the NUMBER button with a number lif2 3 afs e corresponding to the set of observation conditions to be replicated The set of observation conditions for the clicked 7 8 ofiofi 12 NUMBER button is replicated NOTE Please refer to 4 5 Replicating a set of observation conditions saved in the Medium for the procedure for replicating a set of observation conditions saved on the CF card 44 Erasing stored observation conditions Follow the steps described below to erase the observation conditions saved in the COOLSCOPE memory Erasing all sets of observation conditions 1 Click on ALL CLEAR button The confirmation message All clear appears 2 Click on OK button All numbers on the macro image and the all NUMBER butto
101. is displayed on which you can operate the COOLSCOPE Three WEB screens Room 1 Room 2 and Room 3 are available These screens each have their features as described below For operations refer to 3 3 3 WEB screen functions If multiple PCs simultaneously access one COOLSCOPE through a WEB browser operation may slow down e ROOM 1 Images can be displayed at a predetermined time interval during capturing The COOLSCOPE can be controlled on the screen A shorter time interval than that of the ROOM 3 can be selected Therefore a real time image change can be seen on the screen The ROOM 1 screen can be used on the following OSs and browsers Windows 2000 SP4 Windows XP SP2 Internet Explorer 6 02 SP2 e ROOM 2 Images can be displayed at a predetermined time interval during capturing The COOLSCOPE can be controlled on the screen A shorter time interval than that of the ROOM 3 can be selected Therefore a real time image change can be seen on the screen The ROOM 2 screen can be used on the following OSs and browsers Windows 2000 SP4 Windows XP SP2 Internet Explorer 6 02 SP2 Firefox 1 5 0 4 e ROOM 3 Images can be displayed at a predetermined time interval during capturing The COOLSCOPE can be controlled on the screen This screen is not appropriate for real time image observations but has other functions such as Annotation To use the ROOM 1 screen the ActiveX controls must be set up Refer to 3 3 5 Ac
102. isplays the Save menu Note Illustrations to the left of the SAVE button represent the respective output destinations Saves the image data to the CF CARD Saves the image data to the FTP server Prints the image data Note Images can be printed out with a PictBridge compatible printer For information about printing images refer to P73 Saves the image data to the USB memory D Saves the image data to the USB memory E aera Saves the image data to the USB memory F NOTE When a USB memory is set the COOLSCOPE detects the device and the SAVE button and items in SAVE TO area change to the appropriate condition automatically Control buttons Opens closes the tray Right clicking captures the macro image again POWER OFF button Shuts down the COOLSCOPE OF Ff Right clicking shuts down the COOLSCOPE during objective cleaning 29 III Description of the Multi Screen MEMORY buttons Noj Name Function Page cal RECALL button Calls up the observation conditions stored in the P 48 medium CF card or USB memory MEMORY button Stores observation conditions Right clicking enables disables storage of observation conditions of the medium CF card or USB memory 3 ALL CLEAR button Erases all stored observation conditions a 3 Number button Replicates the selected stored observation conditions Right clicking erases the selected stored observation conditio
103. it darker White balance setting button WB Allows setting of the white balance The white balance cannot be set in the case of overexposure or underexposure Confirm that the green bar appears at the exposure level before initiating white balance setting lt White balance setting procedure gt 1 Click on the button to return to the Multi screen 2 Click on a specimen free area of the macro image At this time if the screen is too dark or if white areas appear at intervals use the AE button to make the necessary adjustments 3 Display the Details menu by clicking on the SETTING button 4 Display the CAM menu by clicking on the CAM tag 5 Display the Advn submenu by clicking on the Advn tab 6 Click on the WB button white balance setting button to set the white balance AE lock button AE_L The COOLSCOPE constantly adjusts its exposure to achieve the optimal image brightness Clicking on the AE_L button stops this adjustment thus fixing the screen brightness This button functions identically to the controller s AE_L button Either button can be used to enable or disable the AE_L Gain button GAIN Y R B Allows adjustment of the image brightness and color balance Change the Y level to adjust the image brightness which is not finely adjustable using the exposure correction button CMP Change the R and B levels to adjust the image color balance with reference to G Normally set them at 100
104. justed depending on the subject If an area of interest on the screen is highlighted adjust the exposure so that the distribution will be shifted toward the left If an area of interest is low lighted adjust the exposure so that the distribution will be shifted toward the right Knowing the exact level of exposure that is needed while you adjust the exposure will help to prevent insufficient gradation during image processing Focus indicator Shows the subject s focusing status both in bar and numeric form When adjusting the focus search for a position that provides the largest number of bars and the highest focusing number s63 a VII Details Menu EES REG1 submenu The Reg1 submenu enables registration of metering area shading tone and custom mode settings AE Area all a2 a3 shad ing SO CAL 1 s2 33 s4 35 36 7 TONE 1 t2 t3 t4 t5 t6 7 Camera Sett ings cl c2 c3 c4 c5 cele Info Info box Clicking the Info box displays Save the software keyboard 1 Registering the metering area setting Use the metering area button on Adv1 to create the metering area After the metering area is created the al to a3 buttons become available Select a button to be registered and press Save to complete the registration 2 Calculating and registering the shading correction Perform the shading correction for the illumination and the optical system This function must be executed without a shie
105. k settings on the server side to see if the FTP server function is active Check settings on the server side to see if the server is enabled to write If images are sent across a gateway specify the Gateway address Use the login test function to confirm whether logged in Check settings on the server side to see if the server is enabled to write Check that the USB 1 1 interface board and USB 1 1 hub are based on USB 1 1 and the USB2 0 interface board and USB 2 0 hub are based on USB 2 0 Check that the interface board and the COOLSCOPE are connected with a hub within a level in the tree structure Using a hub within a level is recommended Check that the approved USB cable is used Use the cable based on the USB 1 1 or USB 2 0 to use the COOLSCOPE with USB 1 1 and use the cable based on the USB 2 0 to use it with USB 2 0 Check that the OS version is appropriate for USB interface USB interface is not applicable to the Macintosh Applicable OS Windows 2000 Service Pack 3 SP3 or later Windows XP e Check that the USB host driver made by Microsoft is installed Normal operation may not be performed due to the driver with a USB board e Images are updated every 5 seconds Notice that the latest image may not always be captured if files are read out at intervals shorter than 5 seconds 1554 Warning appears on the PC One of the following troubles occurs after connecting the COOLSCOPE to the PC Although the
106. lding object Press SD CAL to start the shading correction calculation After the calculation s1 to s7 buttons become available Select a button to be registered and press Save to complete the registration If shading is excessive correct calculation data may not be obtained 64 3 4 VII Details Menu Registering the tone setting Specify a user tone on the Adv2 menu After setting the tone t1 to t7 buttons become available Select a button to be registered and press Save to complete the registration Saving a custom mode A set of settings such as various image capture conditions can be saved as a custom mode The saved custom mode can easily be called up using one of the c1 to c7 buttons A custom mode can be saved in accordance with the procedure described below 1 Specify image capture conditions such as the camera operation mode and exposure from the Advi menu NOTE Press Default button to clear all settings back to their factory settings 2 Select the REG tab to move to the REG1 menu 3 Select a custom mode number from c1 through c7 buttons 4 Click on the Info input box and enter characters Up to 16 characters can be entered 5 When you are finished press Save buttons to save the settings Note that if you move to the REG1 menu while the AE_L is enabled AE_L button is displayed red To save the settings after canceling the AE_L press AE_L button to unlock it and then save the sett
107. lems Sun Java Console Internet Options e Select Security gt Local intranet gt Custom Level Complete the Microsoft VM setting e Specify Java permsissions select High safety Low safety or Medium safety Complete the Scripting of Java applets setting e Enable scripting of Java applets Welcome Microsoft Internet Explorer File Edit View Favorites Tools Help Back a x a A P Search 5 2 Favorites 4 2R z ee 3 Address http 128 31 30 36 Internet Options General Security Privacy Content Connections Programs Advanced Select a Web content zone to specify its security settings 7 Settings i O Scripting Intemet Localintranet Trusted sites Restricted Active scripting sites Disable Enable Local intranet Prompt This zone contains all Web sites that Allow paste operations via script are on your organization s intranet Disable Enable Prompt Scripting of Java applets Custom Disable Custom settings Enable To change the settings click Custom Level Prompt To use the recommended settings click Default Level P P liess A bh em bionbian Lill gt Security Settings Security level for this zone Reset custom settings Custom Level Default Level Reset to Medium low v 112 2 VIII Networking Functions Install the Java plug in If a Java plug in to run Ja
108. les on the specimen Slide holder e Set the main power switch at the rear of the COOLSCOPE to the O position to turn off the power If there is a specimen Slide in the ejected slide tray take the slide out of the tray Then set the main power switch to the position to turn on the power Wait for roughly 10 seconds and then press the power switch e Was the COOLSCOPE shut down properly Always shut down the COOLSCOPE by clicking on the POWER OFF button on the screen The same symptom occurs when the remedial procedure is performed in the event that no on screen buttons accept input 152 2 XI Troubleshooting 3 Network Problems Refer to Chapter VIII Networking Functions for details about network settings o rn OOOO eee Cannot connect the system to a network Follow the sequence shown below to check network settings Check network cable connections cables and hub 4 Check the set values COOLSCOPE IP address and PC network settings Check the presence in a LAN ping from the PC Check browser settings Check FTP settings Checking network cable connections lt Checking types of cable gt e Check that the twisted pair cable for L1OBASE T or 100BASE TX category 5 or 5e is used e If the COOLSCOPE is connected to a LAN via a hub check that straight cable is used e If the COOLSCOPE is connected directly to a PC without going through a hub check that crossed cables are used l
109. like to use this option check the followin I Show Unplug Eject icon on the taskbar 2 01 PM Plug in the USB cable and turn on the COOLSCOPE The device will be recognized by the PC again 137 VIII Networking Functions 3 7 4 For Windows XP Installing the Device Driver When the COOLSCOPE is connected to a PC for the first time the PC automatically recognizes the COOLSCOPE and installs the device driver that comes standard with the OS During the installation process messages will be displayed sequentially in the task tray system tray at the lower right corner of the monitor screen indicating that new hardware has been detected You do not need to perform any specific operations Ui Found New Hardware Recycle Bin USB20 Device lows AP Professional cee Bago die 1 40PM i Found New Hardware Recycle Bin lows XP Professional USE Mass Storage Device Peachey valuation copy Build 2505 When the Safely Remove Hardware icon is displayed in the task bar it means the PC has finished installing the driver When the COOLSCOPE is connected to a USB 1 1 compliant USB port the message shown below will be displayed in the task tray system tray This is not an error message The COOLSCOPE will operate as a USB 1 1 device id HI SPEED USB Device Plugged into non HI SPEED USB Hub HI SPEED USB device is plugged into a non HI SPEED USE hub Ey For assistance in solving this problem click t
110. ller closes 2 Right click on Full screen to open the controller Full screen AD VI More Sophisticated Tasks E S Opening closing the help screen You can display the help screen 1 Click on the HELP button 2 The help screen appears 2 The help screen disappears when you click on the HELP button again 15 Displaying hiding the scale You can display the scale on the micro image 1 Click on the SCALE button fp The scale appears in the upper right corner of the micro image Each click on the SCALE button changes the scale indication in the following cycle Scale Scale Scale horizontal gt vertical gt crossshape P Hide fo NOTE When the scale is displayed it can be moved to a desired position Clicking on the micro image displays the scale so that the clicked position is positioned at the scale origin upper left If the scale lies off the micro image display area its display position is adjusted so that it is displayed within the display area Note that the observation position can only be changed using the MOVE button while the scale is displayed Caution The scale is for guidance purposes only Do not use it for measurement 1 6 Changing the scale colors The scale colors can be changed 1 Right click on the SCALE button The scale color changes each time the SCALE button is clicked Displaying hiding focusing lines on the macro image Foc
111. lp function for details 3 Select Tools Internet Options Left click here to open the pull down menu A about blank Microsoft Interneti Explorer Ele Edit View Favorites Tools Help El G Wae e aje a z X ey 3 Back Fonnard Ste Synchronize earch Favorites History Mail Size Print Eat Wind Updat Address ja about blank Meine epee Show Related Links Internet Options Left click here 4 Select Connections 5 Select LAN Settings Left click here General Security Content Connections Programs Advanced Use the Internet Connection Wizard to connect your computer to the Internet m Dial up settings G Weyer diel a connection Biel Whenever a networ Connection 6 hot present Always diel my deraut connection Gurrent default None Bet Wetault M Benam system security check before dialing m Local Area Network LAN settings f LAN Settings Left click here 88 6 7 8 VIII Networking Functions Select Advanced If Advanced is disabled and you cannot select it left click on Use a Proxy Server first A check mark will be displayed allowing you to select Advanced Local Area Network LAND Settings Automatic configuration Automatic configuration may override manual settings To ensure the use of manual settings disable automatic configuration T Au
112. ls menu camera setting network setting Right clicking displays the maintenance menu for service WB button e WB display Sets the white balance e Other than WB display Calls up the camera setting Right clicking switches between button displays WB HE PAP AZ c1 c2 c3 AEL button Locks the automatic exposure 10 aE Thumbnail button Displays image files stored in a medium CF card or P 50 USB memory as thumbnail images 397 III Description of the Multi Screen Observation buttons Moves the observation position fine movement Right clicking moves the observation position coarse movement 3 MAG button Changes the magnification Right click first to change the optical zoom magnification then to perform auto focus Note This function is enabled only when the optical zoom magnification is adjusted from low to high magnification AF button Auto focus Note Always select a magnification of 5x when using the AF button for the first time after replacing the specimen slide Make sure the specimen is positioned at the center of the field of view Right clicking displays the AF area in the micro image 3 FOCUS button Manual focus fine adjustment DIGITAL ZOOM button APERTURE button 3 AE button Adjusts the image brightness 28 III Description of the Multi Screen Image capture buttons Ol 3 SAVE button Saves the image Right clicking d
113. mat the medium CF card or USB memory click this button For details refer to 1 2 Notes on Using an External Recording Medium in Chapter V TiS a EE EE 1 2 3 4 5 VII Details Menu 1 Submenu buttons 2 Return to Window button 3 Date and Time 4 Pointer speed Low Mild High 5 Printer 6 SAVE button Save vefaat 7 Default button Submenu buttons NetWork Settings Additional Settings Allows you to move among submenus Return to Window button Returns you to the CAM menu Date and Time Enter the date and time here NOTE If the main power is turned off and the COOLSCOPE is idle for a long time the clock in the COOLSCOPE may lose time or stop Approximately four days is the limit If the main power is turned off for several days reset the clock Pointer speed Low Mild High Allows selection of a mouse pointer speed Printer e Mag fix Without check Normal print mode Images will be automatically resized to fit the paper and then printed out With check Real 10 print mode Images will be resized in accordance with CMP value setting and then printed out gt 76 6 7 8 VII Details Menu Caution A PictBridge compatible printer has its original magnification for the print out To use the real 10 print function check and see the CMP value setting for your printer e Test Print button e frame Check here to print out
114. ne max 10 char p mSS Fra le s disables FTP client Enable FIP Client IP Authority function External Server 1p Address 192 168 020 001 ping test a Server No 1 User ID anonymous max 10 char Sets the IP address of i l the server Pass Word none max 10 char Sate way 000 000 000 000 login test Save Default HAC Address 00 90 B5 42 00 01 skip 0 1 Z 3l 4 3 6 i 8 9g al be Mes sa Bex Fey Sais a i a a en fi ee Peel aa ea es et eT ae Re Enable FTP Client For image files to be sent to the FTP server always select this check box to enable the FTP client function External Server Set the IP address and the gateway address of the server Server No Five servers can be stored in memory Assign each server a number IP Address Input example User ID Enter a user ID that is needed to log in to the server Pass Word Enter a password that is needed to log in to the server as necessary Gate Way lt Input example If any entry in the IP address consists of two digits or one digit add 0 or 00 at the beginning of it to make each entry consist of three digits as you enter 127 VIII Networking Functions Click the input box and enter an address value If you missed entering any address value hold down skip button on the screen keyboard while you skip to the last entry of the address then enter the correct value again When you finish
115. networked PC through the Web screen we recommend that a fixed IP address be obtained by choosing not to automatically obtain an IP address IP address automatically obtained _ IP address not automatically obtained IP Address Enter the COOLSCOPE s fixed IP address in this box if you choose not to automatically obtain an IP address using the D H C P server Subnet mask Enter the COOLSCOPE s subnet mask in this box if you choose not to automatically obtain an IP address using the D H C P server Example of Internal Server settings e When automatically obtaining an IP address D H C P Auto get an IP lt Make appear IP Address Automatically set after restart Subnet Mask Automatically set after restart NOTE There is a pause before the address is displayed after restart e When specifying a fixed IP address D H C P __ Auto get an IP IP Address lt Example Subnet Mask Example If the IP address contains a one or two digit number add a leading zero 0 or zeros 00 to convert it to a three digit number User ID Password To improve security a user ID and password can be specified for limited access to the COOLSCOPE up to 10 characters If a user ID is specified authentication is requested at the time of connection to the browser It is not possible to log in without entering the user ID and password If the user ID remains anonymous anyone can access the COOLSCOPE Please ke
116. nfinity system Observation information Storable information sets 12 per specimen slide storage function Information stored Observation position focus magnification aperture stop brightness Aperture w 25 to 100 waa a steps 65 to 100 5 steps Focus Manual and auto focus and auto focus Image output Analog RGB SXGA 1 280 X 1 024 60Hz non interlaced Connector Mini D sub 15 pin Image recording Recording medium CF card one 32 MB card supplied Recorded image file format BMP 24 bit JPEG compliant compression mode FINE NORMAL BASIC Image size 2 560 x 1 920 1 280 x 960 Interface CF card Type I Type II USB2 0 host USB mouse USB keyboard USB printer connection USB2 0 device compatible with the Mass storage class connectable to a printer compatible with PictBridge Network IEEE802 3 compliant 10BASE T 100BASE TX DHCP supported HTTP server Telnet server FTP server FTP client Input ratings 100 240 V AC 10 50 60 Hz 1 2 A max Power cord e 100 120 V areas excluding Japan UL Listed detachable cord set 3 conductor grounding Type SVT NO 18 AWG maximum length of 3 m rated at 125 V AC minimum 220 240 V areas EU EN approved 3 conductor power cord set 3 conductor grounding Type HOS5VV F maximum length of 3 m rated at 250 V AC minimum Japan Power cord set that conforms to the Electrical Appliance and Material Control Law with PSE mark 3 conductor grounding Type KP300 KS
117. nge Click Refresh on the View menu of the browser to refresh the image A confirmation dialog box may appear when closing the browser Click OK to close the dialog box Microsoft Internet Explorer x rN It was finished When the Pop up block function is enabled on the browser windows of the application software may be blocked Refer to the browser help and disable the Pop up block function wid VIII Networking Functions Displaying the WEB screen 1 Start the WEB browser e To use the ROOM 1 Enter the IP address of the COOLSCOPE into the address box of the browser example http 192 168 10 2 The WEB screen of the COOLSCOPE appears e To use the ROOM 2 Enter the IP address of the COOLSCOPE and csja htm into the address box of the browser example http 192 168 10 2 csja htm The WEB screen of the COOLSCOPE appears e To use the ROOM 3 Enter the IP address of the COOLSCOPE and cs htm into the address box of the browser example http 192 168 10 2 cs htm The WEB screen of the COOLSCOPE appears For details about the ROOM 1 2 and 3 refer to the following sections O VIII Networking Functions 3 3 3 WEB screen functions The ROOM 1 screen features the following functions Observing images can be displayed at a predetermined time interval and captured to the COOLSCOPE Stream images can be displayed And the COOLSCOPE body can be controlled To use the ROOM 1 screen the ActiveX co
118. ns 5 30 IV Basic Observation Tasks V Basic Observation Tasks To observe a specimen you must follow the steps described below 1 Place a specimen slide in the COOLSCOPE 2 Select an observation position 3 Bring the image into focus 4 Observe the specimen 5 Take out the specimen slide upon completion of observation and image capture This chapter describes the basic observation tasks in the sequence listed above Please refer to Basic Image Capture Tasks in the next chapter for a discussion of these operations ese IV Basic Observation Tasks a Placing a Specimen Slide in the COOLSCOPE 1 Click on the EJECT button on the multi screen You may instead press the gray tray OPEN CLOSE switch at the front of aj the COOLSCOPE The COOLSCOPE tray opens 2 Open the specimen slide holder and place a specimen slide with the cover glass facing specimen slide up on the tray Place it properly making sure that it lies flat a slide holder E NOTE To indicate information of a specimen write it down in the area of the white paper label on the specimen slide The area is located nearside and has an inside height of 15 mm Or use a specimen slide with white frost processed surface and write the information with a black pen 3 Hold down the specimen slide with the IAN holder Check that the holder is closed and the specimen slide is properly fastened 4 Click on the EJECT butt
119. ns in the Memory area are erased Erasing individual sets of observation conditions 1 Right click on the desired NUMBER button The confirmation message Clear appears 2 3 5 The selected number on the macro image and the NUMBER button with the same number in the Memory area are erased Az VI More Sophisticated Tasks Replicating a set of observation conditions saved in the Medium Follow the steps described below to replicate a set of observation conditions stored in the medium CF card or USB memory 1 Put a specimen slide in place and set the medium CF card or USB memory to the COOLSCOPE When observing a live image of the specimen slide exercise caution not to select an incorrect combination of specimen slide and medium When replicating a set of observation conditions using a saved image it is not necessary to place a specimen slide in the COOLSCOPE 2 If any NUMBER buttons are displayed in the Memory area click on the ALL CLEAR button to erase all sets of observation conditions saved in the COOLSCOPE memory Click on OK when the confirmation message All clear is displayed 3 Click on the RECALL button ren A list of Recall folders is displayed Select an observation condition to Recall List of Recall folders NOTE AIl clear the Memory before reading out the media appears if any of the observation conditions remain undeleted from the COOLSCOPE memory The message will dis
120. ntact the distributor Replace the medium Format the medium Turn on the COOLSCOPE again if the problem persists If the problem persists after restart contact the distributor Check whether the CF card is in the COOLSCOPE If the problem persists with the CF card in the COOLSCOPE turn on the COOLSCOPE again If the problem persists after restart contact the distributor Check the cables for proper connection Check the external server s IP address Turn on the COOLSCOPE again if the problem persists If the problem persists after restart contact the distributor Check the cables for proper connection Check the printer power supply Turn on the COOLSCOPE again if the problem persists If the problem persists after restart contact the distributor There is no remedy Recall information is managed by the folder selected at the time of recall registration Do not change the folder configuration There is no remedy Recall information is managed by the folder selected at the time of recall registration Do not change the folder configuration XI Troubleshooting EP Handling Problems If a malfunction is suspected check the following If the problem persists or if the present problem is not shown in the list contact your distributor Unable to turn on the power No image output Micro image or macro image does not appear even when a slide is in the COOLSCOPE Blurred micros
121. ntrols must be set up Refer to 3 3 5 Activex Settings When the ROOM 1 is used for the first time the following security warning dialog box may appear Security Warning Do you want to install and run http 4 128 31 30 70 L2CTL cab signed on 8 30 2006 3 45 PM and distributed by Nikon Corporation Publisher authenticity verified by VeriSign Class 3 Code Signing 2004 CA Caution Nikon Corporation asserts that this content is safe fou should only install view this content if you trust Nikon Corporation to make that assertion CO Always trust content from Nikon Corporation Yes This program is provided with a digital signature for certification Therefore click Yes 17 Micro Size list box 16 Interval Timer Controller list box Micro image 1 RELOAD button 2 WB button Interval a Micro Size Nikon reload COOLSCOPE I WB 3 MOVE button t m Magnification 5 lt 10x 20x 4x7 Focus AFI a D zoom g Aperture AE O Extended Mode Pick Up Image Download Tyee bmp Size Middle 640x480 v 4 MAG button 5 AF button 6 FOCUS button 7 DIGITAL ZOOM button 8 APERTURE button 9 AE button 10 Extended Mode check box 11 DOWNLOAD button 12 TYPE list box 13 SIZE list box 14 QUALITY list Quality Fine Line Red
122. o not discard this manual and keep it handy for easy reference Safety instructions in this manual are marked with the following symbols to highlight their importance For your Safety always follow the instructions marked with these symbols Disregarding instructions marked with this symbol may lead to serious injury or death Disregarding instructions marked with this symbol may lead to injury or property damage Meaning of the symbol indicated on the product The symbol appearing on the product indicates the need for caution at all times during use Always refer to the instruction manual and read the relevant instructions before manipulating any part to which the symbol has been affixed Biohazard AN This symbol labeled on the specimen slide tray calls your attention to the following e Spilling a sample from a specimen slide onto the microscope expose you biohazard e To avoid biohazard do not touch the contaminated part with your bare hands e Decontaminate the contaminated part according to the rules of your facility Caution AN This symbol provided at the following three positions of the COOLSCOPE is intended to call your attention to each of the possible hazards as follows e Tray door The specimen slide tray will come out approximately 10 cm from the front of the COOLSCOPE Do not allow any object or your hand within approximately 10 cm of the front of the COOLSCOPE In addition do not look into the door through which
123. ompliant glass JIS R3702 Thickness No 1 0 12 to 0 17mm No 1 S 0 15 to 0 18mm ISO 8255 Thickness No 1 0 13 to 0 17mm No 1 H 0 15 to 0 17mm Observation method Bright field transmitted observation Possible observation range Entire specimen slide surface 26 X 76 mm Light source White LED 2 3 inch square pixel 524 mega pixels interline CCD 5 9 fps 5M interlace scan 10 0 fps 1 3M interlace scan Image capture device Camera section Exposure control Programmed AE AE lockable Metering method Average metering peak hold metering selecting the location or size of the metering area White balance manual setting type color balance adjustable Color correction gamma tone black level saturation hue special effects monochrome reverse Image correction shading correction noise control edge enhancement 164 XIII Specifications and Confirming Standards Displayed image Macro image Entire specimen slide image Micro image Partially enlarged image Image switching Multi screen with the macro image Full screen without the macro image CF card image reproduction CF card thumbnail display Micro image Optical magnification Optical magnification 5X 10X 20X 40X 2X 4X 20X 40X Digital variable magnification 1X 1 4X 2X 2 8X 4X 16X Scene modes HE stain papanicolaou stain azan stain and 7 custom modes Others Scale display freeze frame image Micro optical system Chromatic aberration free i
124. on and the adjustment of the focus to be performed easier Connection procedure Connect the ergonomic controller D ERG C cable to the AUX IN connector on the rear panel of the COOLSCOPE AUX IN connector 145 Image capturing switch WB switch Z axis speed switch Magnification switch AF switch IX Using Optional Accessories Description of each part and operation procedure XY axis speed switch Y stage knob Magnification switch AE lock switch Screen control D ERG C ERGO CONTROLLER FOR COOLSCOPE Controls Z axis speed switch XY axis speed switch Magnification switch Magnification switch WB switch Image capturing switch AF switch Screen control AE lock switch Focus knob X stage knob Y stage knob LED status Push here to change the Z axis speed between fast and slow The LED lights up when the fast speed is selected Push here to change the X axis and Y axis speed between fast and slow The LED lights up when the fast speed is selected Push here to step down the magnification of the optical system Push here to step up the magnification of the optical system Push here to adjust the white balence of the image Push here to capture an image While capturing the image the LED at the upper left of this switch lights up Push here to focus on the specimen automatically While focusing the LED of this switch lights up Push here to change th
125. on again You may instead press the gray tray OPEN CLOSE switch at the front of the COOLSCOPE The tray closes drawing the specimen slide into the COOLSCOPE Specimen image loading starts automatically At this time the COOLSCOPE is set up as follows e The magnification is set at 5X e In accordance with the 5X magnification lighting brightness and aperture are automatically adjusted 5199 IV Basic Observation Tasks EPE Selecting an Observation Position 1 Click on a desired observation position to be centered on the macro image A micro image of the clicked position is displayed S ae a nea oF ee Micro image F Magnification An enlarged image X f s va oci EASY gt E 5x 10 20 sox n ea eres Sys ps te ne p MEE at the selecte BASSA E DA e Disia position within the f i i HET we E macro image is SAM E A ae wae Gorse o ae displayed Sane WE Hf Ee r aea EH P m C F003 TMAGEOOO BMP EES 31 0CT 10 10 Macro image 3 Bringing the Image into Focus Auto Focusing With 5X selected as magnification bring the image into focus 1 Click on the AF button The image is automatically brought into focus NOTE e When the AF button is used for the first time following specimen slide replacement always select 5 x as the magnification Note also that the AF button must be used with the specimen positioned at the center of the field of view e If it is difficult to
126. ow in the figure Securing ring Enlarged Hole Objective Right side 150 5 6 7 8 9 10 11 XII Service and Maintenance Wipe any stains off the edge lens of the objective e Wear rubber gloves to avoid biohazard contamination e Use a soft clean cotton cloth a lens tissue or gauze slightly dampened with pure alcohol ethyl or methyl alcohol e Handle the pure alcohol carefully in accordance with the manufacturer s directions Reattach the objective and the securing ring at their initial positions To do so screw them in by turning them in the direction indicated by the arrow in the figure Securing ring Hole Objective Detach the left hand objective e To do so detach the left hand cap from the COOLSCOPE using the supplied hex wrench and turn the internal bolt counterclockwise as far as it will go Left side e Push in the objective to the left from the right side window e Detach the securing ring and the objective from the left side window as in the case of the right hand objective Wipe any stains off the edge lens of the objective and attach the objective and securing ring e Wear rubber gloves to avoid biohazard contamination Reattach the covers and secure them in position with rear surface bolts using the Supplied hex wrench Reattach the left hand cap to the COOLSCOPE Insert the power cord plug into the receptacle Then set the main power switch
127. owing the content of each drive n In the following cases there might be a conflict between another connected device and the drive letter e A medium is inserted correctly into position but the message Please insert media is displayed when you click the icon for the media drive e A similar message is displayed when you click the icon for the CCD image drive e When you connect the COOLSCOPE other USB devices already connected to the PC immediately become unusable In such a case change the drive letter assigned to the media drive or the CCD image drive The following shows how to make this change 1 Select Start Control Panel Administrative Tools Computer Management Disk Management 2 Right click the volume whose drive letter you want to change and select Change the drive letter and path 3 Click Edit and choose one of unassigned drive letters from the select box or enter a drive letter directly from the keyboard and click OK 4 Click OK in the ensuing confirmation dialog box 7139 VIII Networking Functions Using the Media Drive Receiving image files Insert a medium into the card slot of the COOLSCOPE and display the explorer screen as shown in the previous section to read data from the medium Or you can read the medium by double clicking the media drive removable disk icon on the My Computer screen The image files on the media drive can be retrieved from the media in the same way for u
128. r 6 02 SP 2 is used on the Windows 2000 SP 4 or the Windows XP SP 2 operating system The setting procedure is as follows 1 In Tools select Internet Options Welcome Microsoft Internet Explorer File Edit view Favorites RRR Help Back P x s 2 http 128 31 30 36 Mail and News gt Pop up Blocker pP 2 2B w Manage Add ons Synchronize Windows Update Windows Messenger Diagnose Connection Problems Sun Java Console Internet Options 2 Select Security gt Local intranet gt Custom Level Perform the settings of ActiveX controls and plug ins e Check the Enable option for Automatic prompting for ActiveX controls e Check the Enable option for Download signed ActiveX controls A Welcome Microsoft Internet Explorer File Edit View Favorites Tools Help Oo Back J x a A d Search 5 2 Favorites 4 B amp 38 Address http 128 31 30 36 i lis cs Internet Options General Security Privacy Content Connections Programs Advanced Select a Web content zone to specify its security settings Security Settings Settings amp 0 Internet Local intranet Trusted sites Restricted sites Internet 4 This zone contains all Web sites you haven t placed in other zones Security level for this zone Custom Custom settings To change the settings click Custom Level To use the re
129. r as necessary Pass Word Enter a password for login to the server as necessary Gate Way Example If the IP address contains a one or two digit number add a leading zero 0 or zeros 00 to convert it to a three digit number ay PE VII Details Menu Ping test Press a ping test button to execute a ping test This function is designed to check whether the specified server exists on the LAN The connection test is targeted for the server shown in the IP Address box under External Server Note that the IP address must be saved in advance by pressing SAVE button If the server responds OK Complete appears The server may not respond to Ping due to security functions such as packet filter firewall and security gateway In such a case restart the COOLSCOPE before disabling the security functions and carrying out the Ping test again Note also that there is no problem with the absence of a server response to Ping if successful login can be confirmed through the Login test which is described below Login test 8 9 10 Press a login test button to execute login test The login test is targeted for the server shown in the IP Address box under External Server and is carried out using the specified user ID and password Note that the IP address must be saved in advance by pressing SAVE button In the event of unsuccessful login check the settings on the PC and the COOLSCOPE settings SAVE button Save
130. ral subjects LINR Linear Use this tone to get a proportional image to the camera This is used to examine the difference in light intensity of photographed data TON6 Metal tissue Use this tone to suppress unevenness in bright parts for high contrast image capturing TON7 Enhanced contrast Use this tone to enhance the contrast Histogram display The COOLSCOPE calculates brightness distribution of the image and displays it as a histogram lis Liss OBE This histogram indicates the distribution of brightness of each R G and B color and can be used for fine adjustment of the exposure level e The horizontal axis indicates the color brightness The leftmost position is the darkest and the rightmost position is the brightest e The vertical axis indicates the frequency of occurrence which is normalized by the maximum value e The average histogram values of each color are shown in numeric form at the right edge and shown by levels at the bottom s62 17 VII Details Menu How to read and set exposure levels If the brightness distribution is concentrated on the right edge of the histogram it means that the highlighted parts of the screen are saturated Conversely if the brightness distribution concentrates on the left edge of the histogram it means that the low lighted parts of the screen are blacked out Although it is ideal if no parts on the screen are saturated or blacked out exposure may have to be ad
131. rme a la norme NMB 003 du Canada Australia EMI directive AS NZS CISPR11 Group1 ClassB In vitro diagnostic medical device directive satisfied GM approved product In vitro diagnostic IVD medical equipment Compliance with which EN standards EN61010 1 EN61010 2 101 EN591 EN1658 and EN60825 1 Low voltage directive satisfied EN61010 1 EN60825 1 EMC Compliance with which EN EMC standards EN61326 EN55011 Group 1 Class B EN61000 3 2 3 3 4 2 4 3 4 4 4 5 4 6 4 8 4 11 Product safety Marking 166
132. rom a distant place of business lt Settings on FTP server PC gt e Check settings on the FTP server Check FTP server functions Check access rights Testing a connection login to the set server After making network settings on the COOLSCOPE perform a ping test and login test e Check the network cable and hub connections e Select the Enable FTP Client check box in Network Settings of the SETUP menu 154 Even though the SAVE button is pressed the timer icon keeps running for about 1 minute and no images are sent Even though the SAVE button is pressed saving is immediately deactivated Images cannot be saved to the FTP server even though the COOLSCOPE is connected to it and no errors are displayed Images cannot be forwarded to the PC via a USB The product cannot be connected with the PC via a USB correctly Same images appear when images on the PC are downloaded continuously via a USB XI Troubleshooting S T This is because the IP address of the External Server that has been set in Network Settings of the SETUP menu cannot be found e Check the IP address of the server one more time e If images are sent across a gateway specify the Gateway address e Use the ping test function to confirm This is because the COOLSCOPE has not logged in to the FTP server e Check that the User ID and Password on the COOLSCOPE side and the user account on the server side match Chec
133. s If leaks occur from the specimen slide decontaminate the contaminated area according to the standard procedure followed by your laboratory Safety Precautions AN ca ution 1 Shut off the power to the COOLSCOPE during maintenance work Always switch off the main power switch and remove the power cord plug from the receptacle before clamping and unclamping movable sections detaching and attaching cables cleaning of the surface of the glass in the tray bottom cleaning of the objective or taking out a specimen slide accidentally left in the COOLSCOPE 2 Avoid contact with water Spilling water onto the COOLSCOPE may cause a short resulting in malfunction or anomalous heating If water is splashed onto the COOLSCOPE immediately switch off the main power switch at the rear set it to the O position and remove the power cord from the receptacle Then wipe off moisture with a dry cloth or something similar If water enters the COOLSCOPE do not use it in this case contact your nearest Nikon representative 3 Do not place any object on top of the COOLSCOPE Do not place any object on top of the COOLSCOPE or cover it with a cloth or the like The system temperature will rise resulting in malfunction 4 Precautions during assembly and installation e Exercise caution not to catch your fingers or hands in the equipment during assembly and installation e The COOLSCOPE weighs around 18 5 kg Hold it firmly by the recesses at its front an
134. s O it does not represent the focusing position 4a Darkening brightening the image You can adjust the image brightness level The desired level can be selected from among a number of available options 2 0 1 6 1 3 1 0 0 6 0 3 0 0 3 0 6 1 0 1 3 1 6 2 0 1 Click on the AE button E The level changes each time the AE button is clicked 5 Taking the Specimen Slide out of the COOLSCOPE Take out the specimen slide upon completion of observation and image capture 1 Click on the EJECT button The COOLSCOPE tray opens 2 Take the specimen slide out of the tray 3 Click on the EJECT button again lt 35 a V Basic Image Capture Tasks Basic Image Capture Tasks To capture the image of a specimen follow the steps described below 1 Set a medium 2 Specify the image file format and other data 3 Capture the specimen image This chapter describes the basic image capture tasks in the sequence listed above Setting a Medium to the COOLSCOPE TO save a captured image a medium such as a CF card or a USB memory must be set to the COOLSCOPE 1 Before capturing an image set a medium such as a CF card or a USB memory CF card slot z Port for a USB memory 4 RAAN A Caution This port is used for a USB memory only Do not connect other USB device such as a USB mouse Caution Be careful about the orientation of the CF card Face the back side upward
135. s the settings Default button Resets the settings to their defaults IP Authority Used to specify IP addresses permitted to connect to the COOLSCOPE To designate IP addresses allowed to connect to the COOLSCOPE display an asterisk and register authorized IP addresses by the procedures given below Up to eight IP addresses Registering IP addresses authorized to connect 1 Insert a CF card into the PC 2 Create a folder named NET on the CF card 3 Create a file and list of IP addresses authorized to connect Example of data 192 168 10 2 192 168 10 10 192 168 10 36 192 168 21 You can use the wild card character In this example the wild card character represents all values from 1 through 254 273a VII Details Menu 4 Assign a name with the ip extension to the text file and save it to the NET folder Example of filename Autho ip 5 Insert the CF card into the COOLSCOPE 6 Connect to the COOLSCOPE from the external PC using Telnet and execute a w_IPA command to register the IP addresses Refer to 3 5 Using Telnet Commands to Operate on the COOLSCOPE in Chapter VIII 74 VII Details Menu 1 Submenu buttons 2 Return to Window button 3 Format button 1 Submenu buttons To show another submenu click here 2 Return to Window button To return to the CAM menu click this button 3 Format button To for
136. ss of recorded data or damage to the CF card e Be sure to use a USB memory whose operation is verified Not all USB memories are compatible with the COOLSCOPE e If a USB memory is not recognized disconnect and reconnect it e Do not connect nor disconnect the USB memory when writing data Caution When a medium is connected to the COOLSCOPE while the COOLSCOPE is being operated the medium is heated by heat generated from the COOLSCOPE A CF card or microdrive is heated more excessively since it is inserted in and connected to the COOLSCOPE When the COOLSCOPE is operated at 40 C maximum operating temperature the operating temperature of a CF card or microdrive becomes approximately 60 C Some media do not guarantee operation under the above operating temperature Do not use such media Medium formatting function The COOLSCOPE can format a medium into a FAT type A FAT type is automatically set in accordance with the size of the medium approx under 2MB FAT 12 approx 2MB to 512MB FAT 16 approx above 512MB FAT 32 The COOLSCOPE will format a medium when you select a medium to be formatted on the File Settings submenu and press the button Terie V Basic Image Capture Tasks Specifying the Image Storage Format Storage Medium File Name and Label Illumination On delivery the COOLSCOPE has the following settings set for image storage format storage medium and filename no onem O os Storage forma
137. sual files on a PC Removing media 1 Check to see that the PC card indicator on the COOLSCOPE is turned off Close the menus displayed on the COOLSCOPE 2 If any image files have been loaded from the media to the PC close all of the open image files on the PC 3 Right click the media drive removable disk icon on the My Computer screen and select Eject P My Computer ek a File Edit wiew Favorites Tools Help Back i pP Search p Folders Fi Address E oo m Shared Documents mi Administrator s Documents System Tasks View system information Hard Disk Driv ae TA Add or remove programs E change a setting en Local Disk C S Local Disk D Other Places 5 Ea Local Disk E Ea Local Disk F My Network Places ap ocal Disk E 7 ocal Disk F E My Documents Shared Documents Devices with Removable Storage g Control Panel T 314 Floppy 4 y XRIPFRE_EN G Details My Computer en a Sa ite n MATSUZAKI H 2 NIKONIMG 1 3 Network Drives yp Psqdata on Psgsery T Al 4 Remove the media manually from the COOLSCOPE 140 VIII Networking Functions Using the CCD Image Drive Double click the CCD image drive removable disk icon on the My Computer screen to access the internal CS1D_IMG folder of the COOLSCOPE The latest image files that have been shot by the COOLSCOPE are stored in the CS1D_IMG folder and these fil
138. t 5 0 Update 9 License License Agreement Please read the Following license agreement carefully Sun Microsystems Inc Binary Code License Agreement for the JAA 2 PLATFORM STANDARD EDITION RUNTIME ENVIRONMENT 5 0 SUN MICROSYSTEMS INC CSUN IS WILLING TO LICENSE THE SOF TAARE IDENTIFIED BELOW TO YOU ONLY UPON THE CONDITION THAT YOU ACCEPT ALL OF THE TERMS CONTAINED IN THIS BINARY CODE LICENSE AGREEMENT AND SUPPLEMENTAL LICENSE TERMS COLLECTIVELY AGREEMENT PLEASE READ THE AGREEMENT CAREFULLY B DOWNLOADING OR INSTALLING THIS SOFTWARE YOU ACCEPT THE TERMS OF THE AGREEMENT INDICATE ACCEPTANCE BY SELECTING THE ACCEPT BUTTON AT THE BOTTOM OF THE AGREEMENT IF YOU ARE NOT WILLING TO BE BOUND B ALL THE TERMS Typical setup All recommended Features will be installed O Custom setup Specify the features to install For advanced users e Clear the check box for the program that will only install the Java plug in then click Next ig J2SE Runtime Environment 5 0 Update 9 Google Google Toolbar 4nd Google Desktop Install free programs with the Java Runtime Environment Search the Web with Google From any site Block annoying pop up ads Autofil web Forms with a single click Privacy Policy Google Desktop aaa oe Instantly Find your email Files media and web history a Search directly From your desktop with the deskbar Privacy Policy By installing these applications vou agree to the appropr
139. t Checking hub connections gt See the instruction manual supplied with the hub for details and follow the description written in it e Do not connect the COOLSCOPE to the Uplink Be aware that Uplink on some hubs is indicated as MDI Uplink 1 x 1 1 x or 8 8 x Also if a hub has cross and straight ports caution must be exercised There is a limited number of useful ports on a hub Be careful when connecting to an empty port because doing so could exceed the limit Checking the set values lt Settings on the COOLSCOPE gt e Make network settings in Network Settings of the COOLSCOPE SETUP menu e g Enable Network or IP Address e Always be sure to make gateway settings if data communication between different network groups is to be performed e g sending or receiving data to and from a distant place of business lt Settings on a PC gt e When connecting from a PC that is not connected to a LAN check the network settings on the PC first Set the IP address and other information specific to the PC 159 Cannot connect the system to a network Continued from the preceding page Cannot access the COOLSCOPE from a browser Images cannot be saved to an FTP server See 3 6 Saving Images to an FTP Server from the COOLSCOPE in Chapter VIII No response after pressing the SAVE button when an FTP server is selected as the image destination XI Troubleshooting
140. t Image file format BMP Folder name Change the image storage format storage medium and or file name as necessary The settings remain in effect until the power switch of the COOLSCOPE is turned off If you use the POWER OFF button to switch off the COOLSCOPE the current settings for image storage format filename and label illumination are saved These settings are reloaded the next time power is switched on For the storage medium the initial value changes depending on the set device The USB memory setting is set when a USB memory is connected and the CF card setting is set when a CF card is inserted 1 Right click on the SAVE button The Settings screen and the software keyboard are displayed fe TYPE EE emp res E irr size E E 1280 960 E E save To cr tc server FOLDER 0o35 FILE NAME ERSE 000 JPG CEUD LABEL M Licht Ok CANCEL Settings Screen 2 Change settings for the following items as necessary TYPE Image file format BMP JPEG TIFF Check a desired file format in which to save the image SIZE Image size 2 560 1 920 1 280 960 Check a desired image size for saving the image 2560 1920 is enabled when Full screen is selected QUALITY Image quality FINE NORMAL BASIC Check a desired image quality when saving the image in JPEG format Be e V Basic Image Capture Tasks SAVE TO Storage medium Check the desired storage medium CF CARD or SERVER FTP server If you wish
141. takes some time after it restarts before the addresses are displayed e When setting a fixed IP address D H C P __ Auto get an IP IP Address 192 168 lt Input example Subnet Mask 255 255 lt Input example If any entry in the IP address consists of two digits or one digit add 0 or 00 at the beginning so that entry will consist of three digits N A Click the input box and enter an address value If you missed entering any address value hold down Skip button on the screen keyboard while you skip to the last entry of the address then reenter the correct value Once you have finished with all the settings press SAVE button and then restart the COOLSCOPE To revert to the initially set value press Default button and then SAVE button and restart the COOLSCOPE 85 VIII Networking Functions Settings for improved security To improve security make the following settings on the Network Settings screen Be aware that the transferred images and information are not encrypted Limiting logins by User ID and Password settings In the Internal Server set a user ID and a password User ID Input up to 10 characters Pass Word Input up to 10 characters Once you set a user ID you will be asked to provide authentication when connecting to the COOLSCOPE from a browser You cannot log in unless you supply your user ID and password If the user ID is defaulted to anonymous anybody c
142. tension bmp to the filename and check the image using the image editing application software 12 Select an image file format ROOM 1 ROOM 2 ROOM 3 A desired image file format can be selected for retrieving the micro image from the COOLSCOPE Two options bmp bitmap jpg JPEG and tiff TIFF are available to choose from for the image file format 1 Click on the TYPE list box and select a desired image file format NOTE The selected image file format does not affect the COOLSCOPE s image file format setting 106 13 14 15 16 VIII Networking Functions Selecting an image size ROOM 1 ROOM 2 ROOM 3 A desired image size can be selected for retrieving the micro image from the COOLSCOPE Two options 1280 960 and 640 480 are available to choose from for the image size 1 Click on the SIZE list box and select a desired image size NOTE The selected image size does not affect the COOLSCOPE s image size setting Selecting a JPEG image quality ROOM 1 ROOM 2 ROOM 3 A desired image quality can be selected when JPEG is selected as the image file format for retrieving the micro image from the COOLSCOPE Three options FINE NORMAL BASIC are available to choose from for the image quality 1 Click on the QUALITY list box and select a desired Qualit image quality pales Changing the color of the cross line on the macro image ROOM 1 ROOM 2 ROOM 3 T
143. the tray comes out e Port for a USB memory This port is used for a USB memory only Do not connect other USB device such as a USB mouse e Left and right side covers Opening the left or right side cover will leave the internal mechanical parts exposed Touching any such parts may result in injury Before opening the left or right side cover set the main power switch at the rear of the COOLSCOPE to the O position to turn off the main power and remove the power cord plug from the receptacle e Rear panel When connecting any peripheral properly connect it to the correct terminal Improper connection may result in malfunction Trademarks Microsoft Windows and Internet Explorer are registered trademarks of Microsoft Corporation in the United States or other countries Macintosh and Mac OS are registered trademarks of Apple Computer inc in the United States or other countries Products and brand names are trademarks or registered trademarks of their respective companies The TM and marks are not used to identify registered trademarks and trademarks in these instructions Safety Precautions Safety Precautions Please follow the safety precautions listed below warning 1 Purpose of use Use the COOLSCOPE for observation and image capture of specimen slides and not for any other purpose The COOLSCOPE is primarily designed to conduct pathological and anatomical examination of cells and tissues as well as to capt
144. the icon is right clicked again the photometry area button is displayed with a frame and you will have a chance to set the metering area Operate the mouse to set the area By dragging the frame of the photometry area you can change the photometry area size By dragging the center of the photometry area you can change the photometry area position When the icon is further right clicked the red frame disappears For registration of the set photometry area refer to Section 1 3 REG1 submenu Metering mode selector button p Average metering VH Peak hold metering A metering mode can be selected to determine the exposure level Normally select Average metering Selectively use these modes as follows as the circumstances demand e When there is little variation in brightness over the entire image under a bright field of view Select Average metering e When making the background white even whiter Select Peak hold metering and select a positive value for exposure correction e When the subject is black in places and the background is almost entirely black Select Peak hold metering 60 9 10 11 12 13 14 VII Details Menu Exposure correction button CMP Allows the screen brightness to be changed Left clicking on the button increases the adjustment value while right clicking on it reduces the value Select a positive value to make the screen brighter and a negative value to make
145. the icon is right clicked again the photometry area button is displayed with a frame and you will have a chance to set the metering area Operate the mouse to set the area By dragging the frame of the photometry area you can change the photometry area size By dragging the center of the photometry area you can change the photometry area position When the icon is further right clicked the red frame disappears For registration of the set photometry area refer to Section 1 3 REG1 submenu Metering mode selector button b Average metering VH Peak hold metering A metering mode can be selected to determine the exposure level Normally select Average metering Selectively use these modes as follows as the circumstances demand e When there is little variation in brightness over the entire image under a bright field of view gt Select Average metering e When making the background white even whiter gt Select Peak hold metering and select a positive value for exposure correction e When the subject is black in places and the background is almost entirely black gt Select Peak hold metering 55 9 10 11 12 13 14 VII Details Menu Exposure correction button CMP Allows the screen brightness to be changed Left clicking on the button increases the adjustment value while right clicking on it reduces the value Select a positive value to make the screen brighter and a negative value to make
146. tional conventions in the manual Abbreviations Personal computer Mouse operation Clicking Moving the mouse pointer to the target item and pressing the left mouse button once Right clicking Moving the mouse pointer to the target item and pressing the right mouse button once Dragging Moving the mouse pointer while pressing and holding the left mouse button siia Contents Contents Salely PECCAULIONS aere ANAE TISA EEA 4 Using the COOELSCOPE ririmerii e E A O A OAE 16 Name and Function of Each COOLSCOPE Part s sasssasssnnansnnsnnsnannannanannsnnnnannnnnnnan 16 Connecting the COOLSCOPE and Peripherals sasssssussnsnsanasnsnnnnannnnnnnsnnanennannsnennan 17 Basic Operationor tne COOLSCOPE oiana E E O E 18 I CONMECUNG EGUIDMGM msisiipie aan n a ei a a a 20 II Starting up and the Shutting down the COOLSCOPE ssssssssrsssssssrsrrrerne 21 1 T rming the Power ON OFF csccsescrecesaevtousanaceacaseeeteneatierscarwbiegastseeeateestaccaesonacares 21 2 SUACUUD 22 tiwocs satiasiwad ttorsaatnusewnedneand es ey neces ee teak enews ed eeint sae ated oe denise sateniwardied 22 3 Inserting and Removing a Specimen Slide cc cc cecce eee eee eee eee nee eeeeneeseeneeeees 23 4 Snutting down the COOUS COPE msiri oirnne wedaenal feds anes reds eds aoe A bins ad 24 Ii Deseription or the MUlIti Sireen acisiiciiiaeeapaau nase eau urna inact opunn Sanaa 25 T Sereen AV O UG seh tates E hohe dele nea i
147. tions When using the COOLSCOPE with the magnification 2X or 4X note the following V Aperture stop The operable aperture stop range is from 65 to 100 Thank you for purchasing a Nikon product This instruction manual is written for users of Nikon COOLSCOPE CS1D To ensure correct usage read this manual carefully before operating the product e No part of this manual may be reproduced or transmitted in any form without prior written permission from Nikon e The contents of this manual are subject to change without notice e Although every effort has been made to ensure the accuracy of this manual errors or inconsistencies may remain If you note any points that are unclear or incorrect please contact your nearest Nikon representative e Some of the equipment described in this manual may not be included in the set you have purchased e If you intend to use any other equipment with this product read the manual for that equipment too e If the equipment is used in a manner not specified by the manufacturer the protection provided by the equipment may be impaired Warning and caution symbols used in this manual Although Nikon product is designed to be completely safe during use incorrect usage or failure to follow the safety instructions provided may cause personal injury or property damage and will lead to the forfeiture of warranty claims To ensure correct usage read the instruction manual carefully before using the product D
148. tivex Settings If the ActiveX controls have already set up but no image can be seen use the ROOM 2 screen To use the ROOM 2 screen the Java applet must be set up Refer to 3 3 6 Java Applet Settings You cannot access the COOLSCOPE with multiple browsers on a PC Usually up to three PCs can access one COOLSCOPE simultaneously at an enough speed When multiple PCs access one COOLSCOPE simultaneously settings for displayed image quality time interval and electronic zoom may differ from the preview settings for the ROOM 1 2 or 3 Images on all browser will be displayed in the condition that the last time setting for the ROOM 1 2 or 3 operated on a PC 90 VIII Networking Functions When the ROOM 1 2 and 3 are simultaneously previewed the preview image of the ROOM 3 may have a white line of a few millimeters width on its bottom But images can be saved without such a line NOTE The screen shots shown in this section may differ depending on which browser software or version is used Regarding the use of a browser Depending on the PC environment and the browser version the WEB screens may behave differently or suffer some operational restrictions Do not use the Back button of the browser If you press the Back button the displayed image will not be refreshed In that case click Refresh on the View menu of the browser to refresh the image In some cases the screen image may be disturbed in a screen cha
149. to select SERVER you must first set up the FTP server in advance Please refer to Chapter VIII Networking Functions for more information on the FTP setup procedure When a USB memory is set the COOLSCOPE detects the device and the SAVE button and items in SAVE TO area change to the appropriate condition automatically FOLDER Folder name The software keyboard appears when the FOLDER text box is clicked Enter a folder name up to 5 characters Software Keyboard A number is assigned to a folder name This number is increased by one each time a specimen slide is ejected and retracted The number starts at 000 and returns to 000 after it reaches 999 RESET No Resetting of folder numbers The start value for the folder numbers for folder names is reset to OOO Caution During image capture after the folder number is reset the newly created image files will overwrite any existing folder on the CF card having the same name Before image capture make sure no folder has the same name as the names assigned to new images FILE NAME File name The software keyboard appears when the FILE NAME text box is clicked Enter a file name up to 5 characters Software Keyboard The number starts at 000 and returns to OOO after it reaches 999 CF USB Formatting the medium Clicking on the FORMAT button deletes data from the medium CF card or USB memory and initializes the medium BO ie V Basic Image Capture Tasks
150. to the table shown below For JPEG images the size varies greatly depending on the image thus please use the table for reference only File Type BMP JPEG JPEG JPEG TIFF Fine Normal Basic 2560 1920 14MB 4MB 1 9MB 1 2MB 14MB 1280 960 3 6MB 1 0MB 480KB 320KB 3 6MB 40 VI More Sophisticated Tasks More Sophisticated Tasks This chapter explains convenient functions not covered in the discussion of basic tasks Displaying the Screen aa Enlarging the macro image quitting enlargement You can display the macro image in the micro image area and enlarge it there 1 Click on the ENLARGE button H The image displayed in the micro image area is switched as follows each time the ENLARGE button is clicked Macro image Macro image right halif left half P Micro image fo sja VI More Sophisticated Tasks Switching between Multi screen and Full screen You can switch between Multi screen and Full screen modes When you select Full screen the micro image will fill the entire screen 1 Click on the DISPLAY button The display switches from Multi screen to Full screen 2 The display switches back to Multi screen when you click on the DISPLAY button again Nikon 6 COOLSCOPE I Multi screen Full screen Closing the controller You can open or close the controller when Full screen is selected 1 Click on the CONTROLLER button The contro
151. tomatically detect settings Use automatic configuration script AGOress Proxy server M Use a proxy server Address Port Advanced l Bypass proxy server for local addresses Left click here onee In the Exceptions column write the IP address of the COOLSCOPE Refer to 3 2 2 Setting Essential Items Network Settings in Chapter VIII for details on the IP address of the COOLSCOPE Enter a period after every three digits of the IP address If the first digits of the IP address are OO or O do not enter the zeros Proxy Settings Servers eee O j Socks CF O O i M Use the same proxy server for all protocols Exceptions Do not use proxy server for addresses beginning with Enter the IP address here Example When entering the IP address 192 168 1 2 Use semicolons to separate entries conest_ Click OK Move the pointer Tt to the position of OK and left click on it Repeat this until the screen returns to the one from step 1 89 3 3 2 VIII Networking Functions Displaying the WEB screen Once a PC and the COOLSCOPE are connected through a network it is possible to check or capture the image that is being shot by the camera head of the COOLSCOPE remotely using a WEB browser The COOLSCOPE can also be controlled When you access the COOLSCOPE from a WEB browser a WEB screen
152. tton If the server cannot be logged in check settings on the PC side and those of the COOLSCOPE 128 VIII Networking Functions 37 Using the COOLSCOPE when Connected to a PC with a USB Cable 3 7 1 Recommended Operating Environment When the COOLSCOPE is connected to your PC with a USB cable you can read or write to the recording media inserted in the COOLSCOPE or capture the live image being shot by the CCD camera onto the PC Supported PCs The COOLSCOPE can only be used with a Windows PC with a USB interface Supported OSs e Windows 2000 Service Pack 3 SP3 or higher e Windows XP If you are using Windows 2000 be sure to update its version to SP3 or higher Otherwise data may not be read or written to correctly About the recording media used by the COOLSCOPE e Usable media Compact flash card Type I and II microdrive e Media formatting When formatting media in your PC make sure it is formatted in FAT12 16 Sometimes these are expressed simply as FAT or in FAT32 Note that depending on the OS used you may not be able to select a format The COOLSCOPE has a function for formatting media The media preformatted in NTFS and so forth that is formats not supported by the COOLSCOPE are formatted in FAT16 T Media formatted in other than NTFS may not be correctly formatted in FAT16 depending on the formats used If so reformat media in FAT12 FAT16 or FAT32 on your computer before use Format
153. u This submenu allows you to set the date and time change the mouse pointer response speed and set up the printer File Settings This submenu allows you to format a medium CF card or USB memory Click on the Return to Window button at the upper right corner of the menu to return to the CAM menu 68 VII Details Menu 3a Power On Settings submenu 1 Submenu buttons PowerOn Settings NetWork Settings File Settings Additional Return to Window 2 Return to Window button PowerOn Settings Display GA etal 3 USB Device Mass W Vendor Fi PictB 4 Display VGA Color Adjust buttons Color Adjust 1 2 3 4 5 6 5 SAVE button DS L2 Ver 100 1000 7036 060321 6 Software version detailed information Submenu buttons To show another submenu click here Return to Window button To return to the CAM menu click this button USB Device Specify the USB device connected to the USB D connector Mass S The USB D connector is connected to the PC to use mass storage function In the mass storage function you can read write from to an external medium set to the COOLSCOPE or take captured images in the PC Vendor This is not available at present PictB The USB D connector is connected to a printer supporting PictBridge to enable direct print of captured images Display VGA Color Adjust buttons To adjust the color balance of the monitor output use these b
154. u has no submenus For more information refer to P 66 SETUP menu The SETUP menu can be used to make settings including network connection date and time and printer connection settings The SETUP menu contains four submenus Power On Settings NetWork Settings File Settings and Additional Settings Clicking on the button at the top of each menu allows you to move among submenus Clicking on the Return to Window button returns you to the CAM or VIEW menu Changes made to the settings remain saved even after the COOLSCOPE is shut down For more information refer to P 68 AD ts button at the oo wal VII Details Menu a CAM menu camera adjustments No camera adjustments are required under normal observation conditions Depending on the Specimen however camera adjustments may provide better observation conditions Adjustments can be made from the CAM menu within the Details menu 1 Click on the SETTING button The Details menu is displayed 2 Display the CAM menu by clicking on CAM at the top of the Details menu 3 The tab located approximately 2 3 of the way up the menu shows which of the two submenus Advi or Adv2 is currently displayed Clicking on this tab allows you to move between these submenus e Advi submenu This submenu allows you to confirm the exposure level and the shutter speed select a metering area and mode correct the exposure set the white balance lock the AE adjust the image quality camera g
155. uired This option provides a sharper image Recommended specimen Cells tissues and general subjects LINR Linear Use this tone to get a proportional image to the camera This is used to examine the difference in light intensity of photographed data TON6 Metal tissue Use this tone to suppress unevenness in bright parts for high contrast image capturing TON7 Enhanced contrast Use this tone to enhance the contrast UTN1 to uTN7 User tones Use these tone settings to register user defined tones Draw a tone curve in Tone Curve Control on the Adv2 menu by dragging the curve The total characteristic of the tone curve will be calculated on the basis of the tone curve displayed in the tone curve control box Therefore to draw a curve from scratch select LINR as the base curve For details on saving a created tone curve refer to Section 1 3 REG1 Submenu Noise suppression buttons NR ON NR OFF Selecting NR ON reduces screen noise thus increasing the clarity of the image Under normal conditions leave NR set to ON Sharpness buttons SHPO to SHP5 Provide a sharper image through outline enhancement of the image Six levels O off to 5 high are available a ee VII Details Menu 19 Effect buttons COLOR B amp W NEGA BlueB and Sepia To apply a color effect use this function e COLOR Standard color image e B amp W Monochrome image e NEGA Photographic negative image This effect is intend
156. unforwarding of the image or clearing of the image by other user do not change the access right setting for the created folder Other settings Network settings and confirmation Network settings and confirmation can also be accomplished by selecting Macintosh HD Applications Utilities Network Utility e The Info tab Shows the IP address and other information e The Ping tab Sends a ping command To use a function that is equivalent to the command prompt in Windows select Macintosh HD Applications Utilities Terminal to display the Terminal screen 126 VIII Networking Functions 3 6 2 Setting External Servers of the COOLSCOPE Network Settings Consult your network administrator for details on the FTP server If you are going to connect the COOLSCOPE to a LAN you need to enable it for network operation and set its own address Setting the FTP client function To set the FTP client function of the COOLSCOPE select Network Settings from the SETUP menu to call up the Network Settings screen shown below PowerOn Settings NetWork Settings File Settings Additional Return to Window NetWork Settings x Enable NetWork Read Only FIP WEB disable Write Control D H C P C Auto get an IP Internal Server ip address 128 031 030 1177 SubNet Haskk 255 255 000 O00 User ID anonymous max 10 char Pass Word no
157. ure images 2 Do not disassemble Disassembly may result in electric shock or malfunction Never attempt to disassemble any sections of the COOLSCOPE other than as specifically described in this manual Contact your distributor if you encounter any problem with your COOLSCOPE 3 Power cord Use one of the power cords specified below Use of an improper power cord can result in fire or other hazard Also note that the COOLSCOPE is classified as subject to class I protection against electrical shock Therefore be sure to connect it to a protective ground terminal e Using units in areas where the supply voltage is 100 to 120 V excluding Japan UL Listed detachable power cord set 3 conductor grounding Type SVT NO 18 AWG 3 m long maximum rated at 125 V AC minimum e Using units in areas where the supply voltage is 220 to 240 V Approved according to EU EN standards 3 conductor grounding Type HOS5VV F 3 m long maximum rated at 250 V AC minimum To prevent electric shock always turn off the main power switch swith to O to the COOLSCOPE before attaching or detaching the power cord 4 Hazardous specimens The COOLSCOPE is intended primarily to conduct microscopic observation and capture images of cells and tissues in the specimen slide Check in advance whether the specimen you will be handling is hazardous Wear rubber gloves if the specimen is identified as hazardous e g infectious in nature Be careful to avoid specimen leak
158. using lines representing the current observation position appear on the macro image You can display or hide these focusing lines 1 Click on the CROSS LINES button Focusing lines disappear from the macro image 2 Focusing lines reappear when you click on the CROSS LINES button again 43 VI More Sophisticated Tasks 18 Changing the colors of focusing lines The colors of focusing lines can be changed 1 Right click on the CROSS LINES button E The color of the focusing lines changes each time the CROSS LINES button is clicked o9 Displaying the AF area on the micro image The auto focus function displays the area in which the image contrast is being detected AF area in rectangular form on the micro image The AF area disappears automatically in approximately one second 1 Right click on the AF button The color of the focusing lines changes each time the CROSS LINES button is AF clicked 2 Adjusting images 24 Zooming out zooming in You can use electronic zoom when Full screen is selected A desired zoom factor can be selected from among the following options 100 140 200 280 400 800 1600 The zoom factor is restored to 100 when you switch to Multi screen 1 Click on the DIGITAL ZOOM button Qala NOTE Executing auto focus clicking on the AF button restores the zoom factor to 100 22 Closing opening the aperture You can close or open the aperture A desire
159. ustments APERTURE Allows aperture adjustment in Allows aperture selection of 25 button 5 increments 50 75 or 100 Macro image AF executes after the observation A micro image of the clicked position is moved After AF is position is displayed completed a micro image is displayed 105 VIII Networking Functions 11 Retrieving the micro image from the COOLSCOPE ROOM 1 ROOM 2 ROOM 3 1 Click on the DOWNLOAD button The Download File dialog box is displayed Pick Up Image Download 2 Click on the SAVE button File Download You are downloading the file md 48 amp 7999970280808080828080 amp 0 From 192 168 10 2 Would you like to open the file or save it to your computer Save Cancel More Info Always ask before opening this type of file NOTE Clicking on OPEN in the Download File dialog box starts up the application software associated with the image file extension If the image is saved using the application software the program will also compress the file resulting in an image quality that differs from the JPEG quality selected using the WEB controller For Mac OS X the following operations are performed when the user clicks the DOWNLOAD button Downloading of JPG file The image editing application software starts automatically and displays an image Downloading of BMP file Although the file without extension may be downloaded the image data is normal Add the ex
160. uttons R_GAIN for R contrast G_GAIN for G contrast and B_GAIN for B contrast SAVE button To save the settings press this button Software version detailed information The characters at the lower right indicate the software version The first three characters numerals 100 in Ver 100 1000 7036 860321 on the example are the version number 69 VII Details Menu 3 2 NetWork Settings submenu PowerOn Settings NetWork Settings File Settings Additional 1 Submenu buttons Raturnoie Mindon 2 Return to Window button NetWork Settings x Enable NetWork 3 Enable NetWork Read Only FTP WEB disable Write Control 4 Read Only 5 Internal Server 6 Enable FTP Client 10 IP Authority 7 External Server 9 Default button D H C P Auto get an IP Internal Server ip Address 128 031 030 177 SubNet Mask 255 255 000 000 User ID anonymous max 10 char Pass Word none max 10 char E Enable FIP Client IP Authority External Server 1P Address 192 168 020 001 ping test Server No 1 User ID anonymous max 10 char Pass Word none max 10 char gateway 000 000 000 000 login test a S SAVE button Save Default 00 a0 R5 42 0N skip 0o 1 2 3 4 5 6 7 8 9 an Pal Bex Bal Se Sel Kaa a a ee a n o pl q r E E w xX vez Caution If the
161. va applets is not installed you will be requested to install a Java plug in the first time you access ROOM2 Follow the instructions displayed to install it e Click Install Internet Explorer Security Warning Do you want to install this software Name jinstall 1_4 windows i566 cab Publisher Sun Microsystems Inc More options Install Don t Install While Files From the Internet can be useful this File type can potentially harm vour computer Only install software From publishers you trust what s the risk Java TM Installer JavalTM Technology Sun Microsystems Inc JavalT M technology powers the best Interactive content on the Web 113 VIII Networking Functions e Select I accept the terms in the license agreement and click Next i Java 2 Runtime Environment SE v1 4 2_11 License License Agreement Please read the following license agreement carefully Sun Microsystems Inc Binary Code License Agreement for the JAVA 2 RUNTIME ENVIRONMENT J2RE STANDARD EDITION VERSION 1 4 2 X PUN MICROSYSTEMS INC CSUN Is WILLING TO LICENSE THE SOFTWARE TENTIFIED BELOW TO YOT OWT Y TRON I do not accept the terms in the license agreement e Select Typical and click Next ig Java 2 Runtime Environment SE v1 4 2_11 Setup Type Setup Type Choose the setup type that best suits your needs Please select a setup type All recommended Features will be installed
162. ve If the media drive can be removed the message shown below is displayed i Safe To Remove Hardware The USB Mass Storage Device device can now be safely removed From the system 4 To remove the CCD image drive follow step 3 5 Unplug the USB cable or turn off the COOLSCOPE Plug in the USB cable and turn on the COOLSCOPE It will be recognized by the PC again 143 VIII Networking Functions 3 8 Using the Direct Print Function 3 8 1 Printout When the left symbol of the SAVE button fell shows E click the SAVE button to send the image directly to the printer 3 8 2 Setting for the direct print function Set the following for the direct print function 1 Check the checkbox of Pict B in the USB Device area in the PowerOn Settings Submenu and then click the SAVE button 2 Right click on the SAVE button l Check the checkbox of Printer in the SAVE TO media and then click the OK button a A macro image cannot be printed out with the direct print function Removing the COOLSCOPE from the PC Because the COOLSCOPE is connected via a USB interface it can be removed from the PC even while the PC remains powered on For details refer to 3 7 5 Removing the COOLSCOPE from the PC 144 IX Using Optional Accessories IX Using Optional Accessories a Ergonomic controller The ergonomic controller enables the movement using the knob of the observation positi
163. wer to the PC main unit and start Windows 2000 or Windows XP 2 Plug the A connector of the USB cable into the USB port of the PC main unit or the hub 3 Plug the B connector of the USB cable into the USB device connector of the COOLSCOPE If this is the first time you are using the COOLSCOPE in Windows the PC automatically recognizes the COOLSCOPE and installs the device driver Refer to the subsequent pages for details i This function is not available unless Mass S is selected in the USB Device section on the Power On Settings screen k When the COOLSCOPE is operated through a network the PC may not recognize the COOLSCOPE In this case the PC cannot read or write data correctly Terminate the network connection And then connect the PC and the COOLSCOPE with a USB cable 131 4 VIII Networking Functions Installing the Device Driver When the COOLSCOPE is connected to a PC for the first time the PC automatically recognizes the COOLSCOPE and installs the device driver that comes standard with the OS During the installation process the window shown below will be displayed sequentially You do not need to perform any specific operations Found New Hardware Found New Hardware Found New Hardware Found New Hardware Found New Hardware Found New Hardware Found New Hardware Found New Hardware 1322 VIII Networking Functions Outline of the Disk Drive When the COOLSCOPE is
164. window Be aware that if you select the Allow Only Anonymous Logon check box no one but an anonymous user can log on When specifying a directory in which to save image files separately for each of multiple COOLSCOPEs connected 1 Select Computer Management Local System Tools Local Users and Groups Users 2 Double click the user you want to set and specify a local path for the home folder on the Profile tab The specified path should be below the home directory however Example If the home directory is C CSFTP the user home folder local path of the COOLSCOPE is C CSFTP CS1 and the FOLDER on the COOLSCOPE side is F then the FTP transfer directory is EC CSFETP CSA E Ete 3 Set local paths for the second and the third users COOLSCOPEs as C CSFTP CS2 and C CSFTP CS3 That way you can specify a separate directory for each user 124 VIII Networking Functions 3 6 1 2 When Using Mac OS X Setup procedure 1 FTP server settings Add the FTP server functions 2 Adda user User ID and Password input Set the COOLSCOPE as a login user Use the user ID of the system manager to log on and perform those settings Settings FTP server settings 1 Select Apple Logo System Environment Settings 2 Click Sharing in the internet and network of the system environment setting window 3 Check the checkbox of Start FTP Service for Mac OS 10 1 or FTP
165. witch between pages by clicking on the button s67 a 10 VII Details Menu File name list This list displays the directories and file names of the images saved in the medium Use the A and V buttons to scroll through the file names Click on a desired file name to reproduce that file SETUP menu network date and time and printer settings The SETUP menu allows you to set up the COOLSCOPE for network connection set the date and time and set up the printer Click on the SETTING button The Details menu is displayed Display the SETUP menu by clicking on SETUP at the top of the menu 1 2 3 4 The SETUP menu contains four submenus Power On Settings NetWork Settings File Settings Additional Settings Clicking on the buttons at the top of the menu allows you to move among submenus Click on the Return to Window button to return to the CAM menu Settings selected in the SETUP menu remain saved even when the COOLSCOPE is shut down Power On Settings This submenu is designed to select the device on the USB D connector to adjust the color balance of the monitor output and to display the version information of the firmware NetWork Settings submenu This submenu is designed to make necessary settings for connecting the COOLSCOPE to the network operating the COOLSCOPE via the network saving image data to the FTP server and viewing COOLSCOPE images from an external PC Additional Settings submen

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