Detection
Contents
1. Q U 3 U N D gt Bb de UN Quantitative analysis of DNA Application O Rapid and reliable analysis of ds ssDNA RNA oligonucleotides proteins and bacterial cell density turbidity measurement Product features O Preprogrammed methods dsDNA ssDNA RNA oligos proteins and OD600 Lowry Bradford and BCA plus micro methods O Ready to use at all times O Measurement cycle of approximately 2 seconds O Storage of the last 100 results and all calibration data O Compact design easy to transport O High quality components ensure high operating safety O Metal housing ensures high stability O Xenon flash lamp has O Automatic calculation of sample dilution O Simple molar conversion using the Conversion key O Up to 10 standards for colorimetric processes extremely long life span 10 years Technical specifications subject to change Description Sophisticated technology in a compact format our BioPhotometer minimizes your workload for everyday routines in the fields of molecular biology and biochemistry Its easy to use keypad greatly facilitates and accelerates operation of the device each method has its own key and parameters such as wavelengths and conver sion factors are set automatically when the method is selected The results of your analysis and all accompanying data are available at a glance a large easy to read display provides immediate information2. A perfect design Choose between two optical path lengths with a single rotation Samples with a normal concentration can be measured using the 10 mm optical path length For samples with a higher concentration simply rotate the UVette by 90 and use the shorter 2 mm optical path length Universal The UVette is ideal for use with the Eppendorf BioPhotometer In other photometers and or spectrophotometers the UVette is inserted with the aid of an adapter This adapter aligns the UVette to the corresponding height of the optical light path in the instrument adapter instrument reference list on page 256 and material resistance data can be found at i Further technical information on absorbance transmission www eppendorf com uvette Material UV transparent plastic free of fluorpolymers and other halogenated hydrocarbons Spectral range 220 1 600 nm Volume 50 2 000 ul Light center height 8 5 mm No of optical windows 4 10 and 2 mm lt 0 5 A at 260 nm 12 5 x 12 5 x 36 mm Optical path lengths Basic absorbance External dimensions W x D x H 4 Technical specifications subject to change Quantitative analysis of DNA UVette certified purity Clean room production and individual packaging ensures that our UVettes are RNase DNA and protein free as well as protected against contamination and dust These cuvettes are ideal for RNA applications and work that is required to be contamination free You can even
3. total yield Warburg formula for protein direct Asso Galerie MEMO For all protein standard calibrations sJuswn4dJsuf 401133130 Results memory For last 100 results absorption and ratio values sample number dilution date and time Interface RS 232 C serial for printer or PC Power requirement Approx 20 W in operation 10 W in standby mode Power supply 120 240 V 50 60 Hz Dimensions W x D x H 20 x 32 x 10 cm Weight Approx 3 kg Optional accessories Thermal printer DPU 414 40 characters line Secondary UV VIS Filter set for verifying photometric and wavelength accuracy Ordering information Description Order no BioPhotometer 6131 000 012 D BioPhotometer PC online software package for online data transfer to PC 6131 810 006 Secondary UV VIS Filter test set for verifying photometric and 6131 928 007 wavelength accuracy NIST traceable Thermal Printer DPU 414 serial printer incl power supply 230 V unit and printer cable 6131 011 006 Printer Paper 5 rolls 0013 021 566 Aspirating pump ECOM P 4153 for rapid emptying of aspiration cuvettes 4153 000 013 with integrated outgoing air filter Eppendorf AG 22331 Hamburg Germany Phone 49 40 53801 0 Fax 49 40 53801 556 3 E Mail eppendorf eppendorf com Internet www eppendorf com Quantitative analysis of DNA Application O Spectrophotometric analyses in the UV and visible range 2220 nm Product features O Suitable for measuring small vo
4. on sample concentration all absorbance readings purity ratios A 7 5s and A 7 539 and sample dilution The BioPhotometer automatically calculates all of these parameters therefore the results are always error free Quantification made easy For further information on photometric quantification see page 288 Ordering information for other voltage options can be found in the appendix on page 304 Quantitative analysis of DNA Interactive BioPhotometer CD The Virtual BioPhotometer is an interactive CD that offers you a guided tour of the Eppendorf BioPhotometer It also contains application notes the user manual and other useful tips and tricks Order your free copy at www biophotometer info Technical specifications Optical system Absorption single beam photometer with reference beam Light source Xenon flash lamp Wavelengths Xe 230 260 280 320 562 595 nm fs j Spectral bandwidth 5 nm 230 320 nm 7 nm 562 595 nm AAN Photometric measuring range 0 000 3 000 A NA N Systematic error 1 ati A I Random error lt 0 5 ati A Method dependent evaluation Absorbance concentration using conversion factor Concentration using calibration with 1 to 10 standards Endpoint calibration 1 standard Linear regression 2 10 standards Nonlinear regression polynom 3rd degree up to 10 standards single double or triple determ Ratio A 7 5 ratio A 2 3 molar concentration
5. 78 006 Eppendorf AG 22331 Hamburg Germany Phone 49 40 53801 0 Fax 49 40 53801 556 E Mail eppendorf eppendorf com Internet www eppendorf com O T p m O y gt AS gt UN er 3 D z m UN Quantitative analysis of DNA The UVette is ideal for use with the Eppendorf BioPhotometer In other common photometers and or spectrophotometers the UVette is inserted with the aid of an adapter which performs two functions It aligns the UVette to the height of the photometer s light beam O lts aperture function optimally guides the light beam through the UVette Quantification made easy For further information on photometric quantification see page 288 Compatibility list which adapter for which device 2 Amersham GeneQuant I II GeneQuant I II Mosciences GeneQuant devices other than 15 mm GeneQuant I II c Ultrospec series 15 mm A Analytik Jena SPECORD series 8 5 mm or 15 mm When using the adjustable cuvette holder 5 for 8 5 mm or 15 mm light path height uy Juno Y SPEKOL series 8 5 mm When using the adjustable cuvette holder Q for 8 5 mm or 15 mm light path height m Biochrom Libra 521522 15 mm Libra S32 S32PC 15mm 2 ge Bio Rad SmartSpec 3000 8 5 mm Eppendorf BioPhotometer No adapter is required a Hitachi U series UBA 410 1500 Only available in Europe For further O information support eppendorf com GeneSpec I II UBA 410 1500 Only available in Eu
6. lumes 250 ul O UV and VIS transparent between 220 nm and 1 600 nm O Volume markings for 500 ul and 1 000 ul O Two optical path lengths 10 mm and 2 mm O Available in individually packaged RNase DNA and protein free quality O Deep lying optical window prevents scratches Technical specifications Tapered cuvette base for optimal filling O Frosted grip for labeling O Ideal for use in the BioPhotometer O Adapters available for use with other common spectrophotometers see page 256 1 US Patent 6249345 ee DNA free e Pr Pr xe Oto Ra eppendorf Q A Oo Oo gt UVette s O y eN e 220 1600 nM 10 mm Description Since its introduction as the first fully UV transparent disposable cuvette the Eppendorf UVette has become an indispensable tool for a wide variety of applications Our vast experience and expertise in the manufacturing of optical precision parts is evident in the UVette s every detail Clearly the safest The plastic of the UVette provides a transparency range from 220 nm to 1 600 nm Pipetting can be monitored clearly and easily through the bubble free crystal clear plastic even with samples as minute as 50 ul in size The funnel shaped base prevents any capillary effects and ensures that the liquid constantly remains in the center of the measuring area Deep lying optical windows provide vastly superior protection against scratches when the UVette is inserted into a photometer
7. recover your samples without the risk of contamination or degradation O Individually packaged for contamination free work and sample recovery O Certified RNase DNA and protein free ee DNA free e Pro e x toy 7 eppendorf 2 ES gt UVette s 0p aol HI e 220 4600 nm Ordering information Description UVettes individually packaged single cuvettes certified RNase DNA and protein free 80 pcs UVette routine pack Eppendorf Quality purity level reclosable box of 200 pcs UVette Starterset includes 80 individually packaged cuvettes certified RNase DNA and protein free UVette routine pack safety in numbers An economical choice for applications that do not necessarily require clean room purity our new UVette routine pack gives you convenient access to each UVette while still safeguarding them against dust and or scratches O Ideal cuvettes for routine applications O Safe storage in a reclosable box Quantification made easy For further information on photometric quantification see page 288 Order no 0030 106 300 0030 106 318 4099 100 007 and one universal adapter for 8 5 mm and 15 mm light path heights e g GeneQuant 1 11 Adapter for photometer spectrophotometer with light path height 8 5 mm 10 mm 15 mm 20 mm GeneQuant I II Cuvette stand Additional adapters on page 256 4099 001 009 4099 002 005 4099 003 001 4099 005 004 4099 004 008 4308 0
8. rope For further information support eppendorf com Jenway Genova Adapter from Jenway Please contact your local Jenway or Techne representative PerkinElmer Lambda series except EZ series 15 mm Lambda EZ 201 and EZ 210 8 5 mm When using the micro cell holder Secomam UVIKON series 15 mm When using the single cell holder For models 943 and XL the 15 mm adapter can also be used for multi cell holder 6 x 6 1 Shimadzu UV series 15 mm When using the standard cell holder UV Multispec 1500 15 mm BioSpec 15 mm Techne Genova Adapter from Jenway Please contact your local Techne or Jenway representative Specgene Adapter from Jenway Please contact your local Techne or Jenway representative Thermo Spectronic BioMate 5 15 mm When using the single cell holder Spectronic Helios series 15 mm When using the single cell holder Unicam UV 1 15 mm Recommended by the manufacturer Eppendorf AG assumes no responsibility for the accuracy and completeness of the manufacturer information Technical specifications subject to change September 2003 So far tested devices For an updated compatibility list please refer to our homepage or contact the Eppendorf Application Support team Technical specifications subject to change
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