Vernier Gas Chromatograph Mini GC
Contents
1. Important The glass syringe is fragile and can be easily damaged Be careful not to bend the needle or bend the plunger If the plunger 1s accidentally pulled out of the glass barrel reinserting it is extremely difficult sometimes impossible 3 Prepare the Vernier Mini GC for data collection a b C Turn on the Mini GC using its on off switch on the left side see Figure 5 Connect the USB cable of the Mini GC to the USB port on your computer or LabQuest Start the data collection program and then choose New from the File menu to ensure software settings are set to default values Click Collect in Logger Pro or tap in LabQuest to bring up the Temperature Pressure profile This screen will look something like this P Data Collection _ Collection Mode Gas Chromatograph Start Temp Pp e Hold Time 200 min Ramp Rate 5 fmn Final Temp s ae BM2 e Eo 15 20 Total Length fo imin Time min Pressure 7 kkPa 100 Temperature C an Figure 1 Temperature Pressure Set the Temperature Pressure values to Start temperature 35C Final temperature Hold time 9 min Total length 15 0 min Pressure 5 0 kPa Select Done to initiate the Mini GC warm up Note A new message will appear Do not inject until GC is ready and the LED on the Mini GC is red The Mini GC will take a few minutes to warm up and stabilize When the Mini GC is read2. The Vernier Mini GC gas chromatograph uses ambient air as the carrier gas supplied from an internal pump The Mini GC may also use an inert carrier gas The connector for an inert gas is located on the rear panel of the Mini GC see Figure 6 To use optional inert carrier gas 1 Remove the plastic cap from the hose barb marked IN 2 and move the cap to the hose barb marked IN 1 2 Connect a supply of inert carrier gas by push fitting 1 8 inch ID flexible plastic tubing onto the IN 2 hose barb 3 Monitoring the live pressure reading on the Mini GC s display adjust the pressure regulator on the carrier gas until the Mini GC displays the desired value Caution Assure that the pressure value does not exceed 21 kPa 3 psig 4 In Logger Pro or LabQuest App software click Collect to open up the temperature pressure profile Set up the temperature profile as desired Set the pressure value to be 0 2 kPa less than the pressure that was set in Step 3 for the carrier gas To return to normal operation 1 Shut off the inert gas supply and remove the tubing from the hose barb on the Mini GC 2 Move the plastic cap from the IN 1 hose barb to the IN 2 hose barb Turning the instrument off The Vernier Mini GC should be turned off with the power switch when not in use We recommend unplugging and disconnecting the power supply and disconnecting the USB cable while the instrument is not in use The chromatograph has a built in timer to turn
3. off heating of the column and pressure from the pump after 2 hours with no communication from the LabQuest or computer 4 0 Maintenance To extend the life and ensure the proper functioning of the Vernier Mini GC there are some routine cleanings and replacements that should be followed 4 1 Column maintenance and or replacement The Restek MXT 1 column is a general purpose column that performs very well for our recommended families of reagents and laboratory exercises It is a stainless steel capillary column 11 meters in length and coated with Siltek to make the stainless steel unreactive MXT 1 is a nonpolar phase that has excellent properties for separating polar compounds If separation efficiency deteriorates conditioning the column with 0 5 uL of acetone or methanol or running the chromatograph for 1 2 hours at 120 C and 20 kPa with air or an inert carrier gas will help to restore proper function If you use our recommended procedures and substances with your Mini GC we think you will get many years of use from the high quality Restek column But if performance deteriorates after time such that the column must be replaced contact Vernier for an RMA number the column cannot be replaced by the user You will then send the unit to an RMA address and be charged a fee for the replacement approximately 475 as of 2009 including return postage Vernier Order Information Column Assembly Replacement order code GC COL 4 2
4. Cleaning the exterior of the case Before cleaning the case disconnect power For routine cleaning of the exterior of the case use mild detergent and a damp cloth Do not allow water to leak inside of the case as this may result in electrical shock or shorts Abrasive cleansers should not be used to clean the instrument 4 3 Replacing the septum The septum should be replaced after approximately 150 injections The septum may be replaced by unscrewing counterclockwise the septum retainer nut Note Two additional Septa were shipped with your new Mini GC in a small Ziploc bag This may be done using a coin or a large screwdriver With the retainer nut removed spear the defective septum with a syringe needle and lift it out Place a new septum flat in the cavity Screw the retainer nut to snug tightness Overtightening the retainer nut will not give a better seal of the septum but will damage the instrument After you have used your original septum and the two replacements you can order additional septa Vernier Order Information Septa Replacement pkg of 4 order code GC SEP 4 4 Replacing the MEMS sensor The Seacoast Science MEMS chemicapacitor sensor 1s a micromachined sensor chip 10 coated with a chemoselective polymer The polymer absorbs analytes exiting the column Analyte absorption by the polymer coating is measured by the detector Appendix A circuitry sa The MEMS sensor should last for more than 2000 hours of operatio
5. Vernier Gas Chromatograph Mini GC Order Code GC MINI Introduction The Vernier Gas Chromatograph or Mini GC is an instrument for separating analyzing and identifying substances contained in a volatile liquid or gaseous sample The Mini GC can detect and distinguish between families of compounds including alcohols aldehydes ketones aromatic hydrocarbons carboxylic acids chlorinated hydrocarbons esters ethers and nitriles see Appendix A for more details It has all of the key components of a traditional gas chromatograph including an injection port temperature and pressure controls a capillary column through which different substances pass at different rates depending on various chemical and physical properties and a sensor for detecting the arrival of compounds It has a patented MEMS chip sensor that allows for room air to be used as a Carrier gas The Mini GC connects to the USB port on a computer or the LabQuest handheld Both Vernier Logger Pro and LabQuest App software allow students to easily control data collection parameters then collect data in real time After the peaks are detected the software allows you to determine retention times and or integrate peaks to help quantify the relative amount of each compound present in the sample What is included with the Mini GC e One Mini GC unit with 11 m Restek GC column installed e AC Power Adapter e One Hamilton Syringe 1 0 uL volume e Two extra Septa a th
6. al negative consequences on human health or on the environment The recycling of materials will help to conserve natural resources For more detail information about recycling this product contact your local city office your disposal service or the place where you purchased it The symbol shown here indicates that this product must not be pzd disposed of in a standard waste container E Vernier Measure Analyze Learn Vernier Software amp Technology 13979 S W Millikan Way e Beaverton OR 97005 2886 Toll Free 888 837 6437 e 503 277 2299 e FAX 503 277 2440 info vernier com e www vernier com Rev 5 18 10 Logger Pro Vernier LabQuest and other marks shown are our registered trademarks in the United States All other marks not owned by us that appear herein are the property of their respective owners who may or may not be affiliated with connected to or sponsored by us sy ue Printed on recycled paper 16
7. cts in materials and workmanship for a period of two years from the date of shipment to the customer Consumable items such as the MEMS sensor the Restek GC column and syringes are excluded from the warranty Damage to any part of the Vernier Mini GC product because of misuse misapplication neglect alteration accident and or operation contrary to design or damage resulting from natural causes is not covered by this warranty Note This equipment has been tested and found to comply with the limits for a Class A digital device pursuant to Part 15 of the FCC Rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instruction manual may cause harmful interference to radio communications Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be required to correct the interference at his own expense Disposal Instruction When disposing of this electronic product do not treat it as household waste Its disposal is subject to regulations that vary by country and region This item should be given to an applicable collection point for the recycling of electrical and electronic equipment By ensuring that this product is disposed of correctly you help prevent potenti
8. e Requirements Logger Pro 3 version 3 8 or newer software is required if you are using a computer LabQuest App version 1 3 ooa or newer is required if you are usinga LabQuest You can check the LabQuest version by tapping the 500 Home icon tapping Control Panel and then tapping System Information If you own an earlier version of Tine ein Logger Pro 3 software or LabQuest App you can upgrade to the current version at no cost by visiting our web site for updates www vernier com downloads Note If you use National Instruments LabVIEW software you can obtain a LabVIEW VI for collecting with the Mini GC Go to www vernier com labview Using the Mini GC with a Computer or with LabQuest 1 If you will be collecting data on a computer be sure you are using Logger Pro software version 3 8 or newer If you will be collecting data on LabQuest make sure the LabQuest unit has been updated to version 1 3 or newer 2 For your initial trial obtain a glass syringe and a set of vials of one or more of the following five ketones acetone 2 butanone 2 pentanone 2 hexanone or 4 methyl 2 pentanone or a mixture of any of these 5 ketones You will test acetone and also use it to clean the syringe needle Note More detail on this experiment can be found in Experiment 1 Using a Gas Chromatograph Identifying Unknown Compounds in the accompanying lab manual Gas Chromatography Investigations with the Mini GC
9. g your sample gently wipe the needle from barrel to tip with a Kimwipe 7 Prepare for injection and the start of data collection It is important for you and your lab partner to divide the tasks in this step One person will operate the syringe and the other person will operate the computer controls a When the Mini GC has reached the correct start temperature and pressure the message reads Inject and select Collect simultaneously and the LED on the Mini GC is green b To insert the needle of the syringe into the injection port of the Mini GC hold the syringe with one hand and steady the needle with your other hand Figure 3 Important Supporting the needle guard and the lower part of the needle with two fingers as shown in Figure 3 can prevent the possible bending of the needle Insert the needle into the injection port until the needle stop is fully seated If the needle sticks rotate it slightly while inserting Do not move the plunger yet c Simultaneously depress the syringe plunger and select Collect to begin data collection Pull the needle out of the injection port immediately 8 While the data collection proceeds repeat Step 4 to thoroughly clean the syringe and needle It may take more than three flushes to feel the syringe plunger move smoothly again which is your indicator that the syringe and needle are both suitably clean 9 Data collection will end after fifteen minutes 10 Analyze yo
10. he Syringe 1 Never force the plunger 2 Clean the syringe following the procedure described in Rinsing the Syringe using an appropriate solvent Choose a solvent that will dissolve the contaminating substances Common choices include acetone methanol methylene chloride and acetonitrile but other solvents may used Non alkaline non phosphate non detergent solvents are preferred Do not soak the syringe in any solvent 3 If the plunger is catching during injection 1t may need to be flushed with multiple cleaning solvents Troubleshooting Syringe plunger won t fully depress 1 Follow the procedure for cleaning the syringe 2 Inspect the plunger If it is bent and cannot be straightened dispose of the syringe according to your sharps disposal procedure Appendix D Relationship of Pressure and Column Flow Rate If you are accustomed to setting flow rate rather than column pressure values in a temperature pressure profile here is an equation and a table of typical values representing the nearly linear relationship between these two variables Flow Rate mL min 0 54 x Pressure kPa Pressure kPa Flowrate mL min 2 5 1 4 5 0 2 7 7 5 4 1 10 0 5 4 12 5 6 8 15 0 8 1 17 5 9 5 20 0 10 8 This relationship was determined by measuring the flow rate of the exiting gas air when the column was heated at a constant 45 C temperature at various pressures 15 Warranty Vernier warrants this product to be free from defe
11. inciples of Operation The Vernier Mini GC is designed to separate mixtures of gases or volatile liquids and identify components of the mixtures by their specific retention times The chromatograph uses ambient air supplied from a pump to carry a small sample of vapor through a stainless steel column The chromatograph can used with traditional carrier gases such as helium and nitrogen The column is a general purpose column designed to study solvent impurities distillation gases natural gas odorants sulfur compounds essential oils hydrocarbons semivolatiles pesticides and oxygenates The column is heated using an electric current Temperature of the column is monitored by a built in resistance temperature detector RTD for accurate temperature measurement The column assembly also has an independent thermistor to protect against overheating At the end of the column is a Seacoast Science chemicapacitor sensor The sensor is a micromachined sensor chip coated with a chemoselective polymer The polymer absorbs analytes exiting the column Analyte absorption by the polymer coating is measured by the detector circuitry While the sensor is designed to detect a wide range of analytes it does not detect low polarity compounds such as alkanes 2 0 Specifications The Vernier Mini GC is designed for use in an educational or research laboratory Ambient temperature range for safe operation is 5 C to 40 C and safe range of ambient relative humid
12. ird Septum is already installed in your Mini GC e Two extra Mini GC intake valve plastic caps a third cap is already on the unit e One USB cable e One Lab Manual Gas Chromatography Investigations with the Mini GC e User s Guide this document e One carrying case Important We strongly recommend that you read the entire manual thoroughly before using the Mini GC for the first time There are precautions and usage suggestions throughout this manual that are important to know about prior to doing your first data collection Please wear the appropriate personal safety equipment and use the instrument in accordance with the instructions in this manual to prevent any injury A Caution The Vernier Mini GC gas chromatograph is designed to analyze volatile compounds These compounds may be toxic or highly flammable therefore follow the manufacturer s instructions when operating this instrument Serious personal injury may result from improper use of this instrument Due to the small amounts of analytes there is no hazard that exhaust from the chromatograph could cause a fire when used according to the instructions To minimize exposure to vapors use this instrument in well ventilated areas Refer to the Material Safety Data Sheet for each compound to be injected into the chromatograph For personal protection we recommend that open reagent containers be handled in a hood or well ventilated area Logger Pro 3 or LabQuest App Softwar
13. ity of 0 to 95 The Mini GC should not be immersed or sprayed with liquids Power for the chromatograph is supplied by an external power supply The Mini GC has a back lit liquid crystal display that advises the user as to its current status 2 1 Chromatograph Hardware 11 meter general purpose chromatography column coated with a silicone polymer Column inner diameter is 0 53 mm 30 C to 120 C with a maximum heating ramp of 10 C min Sensor Chemicapacitive sensor Dimensions Weight 108 mm height 191 mm length 133 mm width 1 3 kg 24 VDC 2 5 A from an external power supply Column operating temperature Power requirements Utility External power supply rated for 100 240 VAC 1 5 A 50 60 Hz Carrier gas Ambient air or optional inert gas Environmental conditions Temperature 5 C to 40 C Humidity 95 or less non condensing environment Liquid Injection volume 0 01 to 1 0uL Operating pressure above ambient 1 to 21 kPa pressure 3 0 Installation The Vernier Mini GC should be used in a room that is well ventilated to allow for normal heating and cooling Do not use the Mini GC in the presence of flammable vapors 3 1 Unpacking and Setup Open the lid of the Mini GC carrying case Remove the power supply USB cable and the Mini GC unit from the carton Remove the ties holding the electrical cords You may wish to store separately the Gas Chromatography Investigations lab book the 2 extra septa and 2 extra
14. n If performance Classes of Compounds That Can Be Used in Vernier Mini GC deteriorates after time such that the MEMS sensor must be replaced contact Vernier Typical Range of Acceptable Boiling for an RMA number the column cannot be replaced by the user You will then send Compound Type Compounds Points C the unit to an RMA address and be charged a fee for the replacement approximately 300 as of 2009 including return postage Alcohols C Cs 65 195 Vernier Order Information Sensor Replacement order code GC SENS Aldehydes CGC 20 170 4 5 Storing the instrument Aromatic hydrocarbons C Cyj9 80 165 Proper storage of the Vernier Mini GC will protect it from damage and maintain the life of the instrument The manufacturer recommends flushing the column with Carboxylic Acids C C4 100 165 0 4 uL acetone or methanol followed by 15 min purge at 120 C and 20 kPa Store Esters CU 30 120 the instrument in the protective case provided with instrument Store in a cool dry environment Ethers C4 Cs 35 142 5 0 GC Troubleshooting Ketones C3 Cs 55 175 Nitriles C C 80 140 Error Possiblecauses Appendix B Inconsistent flow Check and replace septum To comply with certification requirements for this instrument the manufacturer Inconsistent peak areas Check injection volume septum provides the following risk assessment Inconsistent retention times Check septum pump Leakage There is no risk from reagent leaks when using this ins
15. ontrolled through the LabQuest or computer software The LabQuest App or Logger Pro software allows the user to 1 Set a temperature profile either using the default settings or as described in student lab instructions 2 Set a desired pressure to run the system maximum of 20 kPa 3 Start and stop a run 4 Name a run and store the data 5 Integrate the peaks and determine retention times Analytes are injected into the instrument through the injection port Optimal results are obtained using the supplied 1 0 uL syringes for liquid analytes Instructions for using syringes are contained in Appendix C A Caution 1 DO NOT inject more than 1 0 uL liquid analyte injecting excess liquid may damage the sensor Note Much larger head space gas volumes can safely be injected up to 1 mL 2 DO NOT lose the rubber cap on the gas inlet on the rear panel of the instrument the instrument cannot develop pressure with this cap removed two extra caps were shipped with your unit 3 DO NOT inject the following liquids substances into the Mini GC Water or compounds with significant amounts of water impurity Amines or any other alkaline substances Liquids containing compounds with large molecular weights larger than the molecular weight ranges shown in Appendix A These types of compounds can become deposited in your Mini GC s column and degrade its performance 3 4 Configuring the instrument for optional carrier gas
16. plastic valve caps for use at a later time To avoid damage to the Vernier Mini GC place the instrument on a flat surface away from 1 Excessive dust 2 Liquids or mists 3 Strong vibration 4 Strong magnetic fields electric fields and or high frequencies Figure 6 shows the rear panel of the instrument with a view of hose connections e OUT the OUT connection is the exhaust port and should be directed away from the user e IN 1 is the inlet for room air used during normal operation of the Mini GC Vernier sells an optional cartridge to dry the carrier air Inlet Air Drying Assembly order code GC DRY e IN 2 1s an inlet for inert carrier gas optional see section 3 4 of this booklet Figure 6 Rear view of Vernier Mini GC 3 2 Powering up instrument Explanation of symbols USB connector to LabQuest or computer Power connector 24 Volt DC 3 A Power PO Poweroff o To operate the gas chromatograph plug the low voltage jack of the power supply into the power socket on the side of the gas chromatograph insert the 120 VAC plug into an outlet Connect the mini jack of the USB cable to the instrument and the standard USB jack into the Vernier LabQuest handheld or computer with Logger Pro Turn on the power switch for both the Mini GC located on left side panel and the LabQuest or computer The order in which you turn them on does not matter 3 3 Using the Vernier Mini GC Operation of the GC 1s c
17. plunger too rapidly can cause the plunger to bend In the case of our 1 0 uL syringe the plunger is a fine wire that extends into the needle A bend in the plunger of this syringe may not be visible but the plunger will begin to stick which can render the syringe useless 3 If the plunger starts to catch in the syringe during sample collection or injection follow the procedure for cleaning the syringe NEVER force the plunger 4 If the syringe becomes clogged do not pump the plunger or attempt to force liquid or compressed air through it The excessive pressure could cause the barrel to crack 5 Avoid unnecessary movement of the plunger in a dry syringe 6 The plunger should only be grasped by the button since any abrasions scratches or oil from one s fingers can interfere with proper plunger operation 7 Always proceed carefully when inserting the syringe needle into the gas chromatograph Occasionally the needle will catch on the column inserting the needle with too much force will cause it to bend which will destroy the syringe Rotating the syringe will alleviate this problem 8 When wiping the needle of the syringe pinch a lint free wipe around the needle next to the barrel of the syringe and wipe in a single motion toward the point of the needle Never wipe the needle toward the barrel 9 Syringes should be regularly inspected for damage including hairline cracks Be sure to check the needle tip for barbs which tear
18. solution and replace the cap on the sample container 4 Holding the syringe vertically at eye level carefully depress the plunger until the syringe holds only the desired injection volume N Carefully wipe the syringe needle with a fresh lint free tissue making sure the tissue does not absorb any sample from inside the needle 6 When you are satisfied with the volume of your sample proceed to injection If not carefully eject the syringe contents into the appropriate waste receptacle wipe the needle with a fresh lint free tissue and begin again at step 2 Injection into the Gas Chromatograph 1 Wait for the Mini GC s status indicator light to turn green and the message Ready for Injection to appear before collecting your sample into the syringe 2 As soon as your sample is loaded into the syringe grip the syringe by the flange and pinch the needle about halfway along its length 3 Aim the needle into the center of the brass needle guide at the top of the gas 14 chromatograph and slowly push the needle into the injection port being careful not to move the plunger If the needle should catch immediately stop pushing and turn the syringe a quarter turn then resume pressure 4 As soon as the syringe is fully inserted against the needle guard quickly depress the plunger and simultaneously click Collect in the data collection software 5 Immediately pull the syringe out of the gas chromatograph Cleaning t
19. the septum and can produce particles that clog the needle or the chromatograph s column Cracked syringes should be discarded according to your sharps disposal procedure Needle burrs can be smoothed with a fine emery board or carborundum 10 Before storing a syringe rinse it with acetone Wipe dry all external surfaces Store the syringe in original shipping container or shock absorbing padding 11 NEVER soak the syringe for an extended period in a solvent Doing so could cause any adhesives used in the construction of the syringe to dissolve Tips 1 Greatest analytical accuracy is achieved when the injection volume is at least 20 of the working volume of the syringe If the optimal syringe size is unavailable be aware that your results will show greater variability 2 Prior to injection moving the syringe plunger as slowly as possible during all steps whether liquid is involved or not will help maintain the accuracy of the injection volume 3 When collecting your sample grip the syringe by the flange and plunger button only as your body heat can affect the volume collected 4 During insertion of the syringe needle into the gas chromatograph support the needle with one hand and hold the flange of the syringe in the other This reduces the chance of bending the needle 13 5 If the needle seems to catch when inserting it into the gas chromatograph turn the syringe a quarter turn and then try again 6 When injecting in
20. to the GC you want to depress the plunger quickly enough to ensure that the entire sample is delivered at once but carefully enough that you do not bend the plunger if the plunger sticks The larger injection sizes require greater care during the injection Rinsing the Syringe 1 Submerge the tip of the syringe needle in the rinse liquid commonly acetone or ethanol and draw the plunger back to 50 of its total volume 2 Remove the syringe from the rinse liquid and place the tip of the needle over an appropriate waste receptacle such as a beaker or lint free tissue Slowly depress the plunger ejecting all of the solution from the syringe Wipe the needle of the syringe with a fresh lint free tissue Repeat the above steps 3 times N Nn BB U Remove the syringe tip from the solution replace the cap of the vial of rinse liquid and wipe the needle of the syringe with a fresh lint free tissue Collecting a Sample 1 Remove the cap from the sample container and rinse the syringe with the solution to be injected following the procedure outlined in the section Rinsing the Syringe 2 Submerge the tip of the needle in the solution to be injected and slowly draw the plunger back by an amount larger than the intended injection volume This excess volume will typically be somewhere around 0 1 uL Example if you are attempting a 0 2 uL injection you should withdraw between the 0 25 and 0 3 uL 3 Remove the syringe tip from the
21. trument as are using correct software versions Fire flammability To minimize risk of fire the exhaust from the chromatograph is Check to make sure power plugs are vented at the rear panel of the instrument In the unlikely event that a large injection olugged in properly 1 0 uL of a very flammable material such as hexane was to ignite by an ignition source placed near the sensor outlet barb the reaction would produce 3 5 calories of energy This energy is enough to raise the temperature of a gram of water 3 5 C or to raise the temperature of the chromatograph 1 3 kg mass by approximately 0 0035 C This is very small when compared against roughly 252 calories released from ignition of one blue tip match Electrical shock To minimize the hazard of electrical shock the chromatograph is powered at 24 V Use only the power supply provided by the manufacturer to power this instrument Appendix C Syringe Usage Instructions 1 0 uL GC Syringe Syringe Handling 1 Never pull the plunger back more than 80 of its total volume Warning Once the plunger has been pulled out it is almost impossible reinsert You may want to err on the side of caution and never have students pull the plunger back more than 50 0 5 uL This avoids having students accidentally pull the 11 12 plunger from the syringe body a costly mistake 2 Exercise caution when depressing the plunger Stickier chemicals can jam the syringe and depressing the
22. ur chromatogram a Choose Peak Integration from the Analyze menu b Select and integrate the left most peak To do this drag from a little before the peak to a point far enough to the right that includes all of the peak Then choose Add Note Prior to integrating it is also possible to drag across a peak and then use the Zoom button to zoom in on the peak Peak Integration 15 Bag Retention Time min Area Area 7 160 920 42 72 07 Time min Cs Remove Lo _ cancel _ Figure 4 Peak integration in Vernier software c To analyze another peak on the same graph repeat Step b d When you are finished with all peaks select OK to return to the graph 11 Using Logger Pro or LabQuest App you can do any of the following a Youcan choose to Store a run In Logger Pro choose Store Latest Run from the Experiment menu In LabQuest App tap the File Cabinet icon b You can choose to save this chromatogram and peak analysis for later use with a unique file name by choosing Save from the File menu c Print your chromatogram and peak analysis table d You can rename run names or peak names in the software 1 0 Product Description and Principles of Operation Figure 5 Side view of Vernier Mini GC Figure 1 shows a side view of the Vernier Mini GC The power input jack power switch USB connection to the computer or LabQuest and column ventilation grill are shown in this view Pr
23. y for injection in Step 7 the message will read Inject 1 Pressure values entered here represent the pressure above ambient air pressure and select Collect simultaneously and the LED will turn to green Continue with Step 4 during warm up 4 Follow the steps below to clean and flush the syringe with acetone Important The glass syringe is fragile Be careful not to bend the needle or bend the plunger Never pull the plunger back more than 50 of its total volume Be careful not to bend the plunger as you press it down a Depress the plunger fully b Submerge the tip of the syringe needle into the vial of acetone c Pull back the plunger to fill the barrel about 1 3 full of acetone d Expel the liquid onto a Kimwipe or a paper towel e Repeat Steps a d at least two times until you are comfortable pulling up a liquid into the syringe and measuring the volume in the syringe barrel Use a Kimwipe or a paper towel to carefully pat around the tip of the syringe needle 5 Follow the process in Step 4 to clean and flush the syringe with 2 butanone or another available ketone the first ketone sample to be injected into the Mini GC 6 Collect a volume of 2 butanone for injection a Submerge the needle into the vial of 2 butanone one last time b Draw up approximately 0 2 uL of liquid It is not critical that the volume be exactly 0 2 uL a tiny bit more or less volume is all right Figure 2 c After collectin
Download Pdf Manuals
Related Search