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LSX-213 G2+ and LSX-266 Laser Ablation System Operator`s Manual

1. HelEx Active 2 Volume Cell NOW QB e PC UP Gas Inlet Sample Outlet Figure 3 20 LSX 213 G2 Argon Only Gas Management State HelEx Cell No He Flow If Mass Flow Controller 3 is not present the HelEx cell will operate in Passive Mode Vent Carrier Gas In om pa Out ERB Pressure Relief Valve Sample J Argon Figure 3 21 LSX 266 Online gas management state 58 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 3 Using the DigiLaz G2 Software Vent Carrier Gas In H H DARE Sample Out TE Pressure Relief Valve Sample i Argon Figure 3 22 LSX 266 Bypass gas management state Vent Carrier Gas In pga Sample Out 713 S Pressure p Relief a c 1 Valve A Sample i Argon Figure 3 23 LSX 266 Purge gas management state 59 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 3 Using the DigiLaz G2 Software Video Operations The Video Operations tab contains controls for adjusting the appearance of the display and for capturing images Gas Managemert Video Operations Lighting Controls Camera Controls Triggering v Toggle Cross Hairs V Toggle Lines dls ve Cross Hair Color Method Color Snap Shot Record Stop Create Sample Map Cancel Sample Mapping Cross Hairs Lines Figure 3 24 DigiLaz G2 Software Video Operations Tab Cross Hairs and Lines The checkboxes control the visibility of the cross hairs and of any methods which have been created The
2. Fuses The only fuses in the laser ablation system are in the laser cooler power supply The laser cabinet has no replaceable fuses See page 108 for fuse replacement instructions and fuse ratings 123 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 7 Safety and Regulatory Information E Safety Notices If the equipment is used in a manner not specified by Teledyne CETAC Technologies the protection provided the equipment may be impaired Repair or service that this not covered in this manual should only be performed by qualified personnel Laser Safety This instrument complies with appropriate safety standards With specific regard to the laser the equipment complies with laser product performance standards set by government agencies as a Class I laser product It does not emit hazardous light the beam is totally enclosed during all modes of customer operation and maintenance U S Regulations Class I Laser Product This instrument is certified to comply with laser product performance standards set by the U S Department of Health and Human Services as a Class I laser product with interlocks and guarding This means that this is a class of laser product that does not emit hazardous laser radiation this is possible only because the laser beam is totally enclosed during all modes of customer operation The laser produces a beam that if looked into could cause eye damage Service procedures must be foll
3. p 73 Spot Size Microns 40 4 gt Laser Energy my o et eet Method foe ee Parameters Shutter Delay 6 10 g Scan Rate microns s 50 00 zd Ans Controls RTA tf c E sf X Y c Focus Stage T el T Controls Defocus sec Defocus Axis Location met x Y Z Polarizer Total Sequence Run Tme Energy N A A e Helium 1 Flow 0 mL min Fluence N A Other Axs Controls Helum 2 Flow 0 mL min Irradiance N A Home Center Load Additonal Flow 0 mL min X 240 14 Y 738 89 Laser Interlock Closed 1 0 Figure 1 5 Sample Viewing Within the DigiLaz G2 Software Laser Head and Optics The laser head is located in the upper chamber of the laser cabinet Itis a compact rigid hermetically sealed unit with a Compact Folded Resonator CFR geometry and is designed to military specifications All of the optical elements are kept in precise relative alignment on a single stable optical table that is mechanically isolated from the rest of the system to minimize any environmental influence on the laser or optics The laser system uses an integrated cooler power supply to prevent overheating which improves laser stability and reliability Equipment Supplied gt LSX 213 G2 or LSX 266 laser cabinet described in this manual gt DigiLaz G2 software described in this manual 12 Vv VV Y V WV CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 1 Introduction Sample cell a single volume standard c
4. General Operations Gas Managemert Video Operations Lighting Controls Triggering LETT Target Edit 333 PSREN Raster N i9 Draw Measure Manual Laser Control Launch Sequence Editor i i Start Sequence Stop Sequence UU Video Mode Method Selection Drawn Method Parameters i P Spot Size Microns 40 v 4 Space Between Spots Microns 10 4l Laser Energy 96 100 s Laser Shot Frequency Hz 20 Shutter Delay s 10 E Burst Count 100 to Axis Controls tT Focus 6 Right click anywhere on raster and choose Expand Raster Main Sequence Depth Profile Cid 4159 47 6581 76 108 1667 25 5840 00 5840 00 _ Rasterj san 10 a Cut From Grid Copy From Grid Paste To Grid xX Delete Row s IE Fill Down 10 Go to Selection oon nM A WN m 87 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 4 Software Methods a Te He General i _ Sequence Editor i Editing Sequence Help a M New Sequence Open Sequence Save Sequence Export To Excel Import From Excel Print Sequence Main Sequence Depth Profile 4159 47 4269 47 4379 47 4489 47 4599 47 4709 47 4819 47 4929 47 5039 47 5149 47 5259 47 5369 47 5479 47 5589 47 5699 47 5809 47 5919 47 6029 47 6139 47 6249 47 6359 47 6469 47 JES 2 3 4 5 6 7 8 10 11 12 13 S amp SeCaa amp x e U Print Setup J Edit the indi
5. Bowe DigiLaz G2 ys e lm E General Operations Gas Managemert Video Operations Lighting Controls Camera Controls Triggering S ETT Target Edit N ABs t Single Line Scan ET l Draw Measure Manual Laser Control Launch Sequence Editor Start Ablation Figure 3 7 DigiLaz G2 Software General Operations Tab Laser Controls Click Manual Laser Control to open this window 49 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 3 Using the DigiLaz G2 Software Spot Size Microns Laser Energy 5 Laser Shot Frequency Hz Continuous Burst Count 100 Delay s 10 Start Laser Figure 3 8 Manual Laser Control Menu Table 3 1 Laser Controls Control What it does Spot size Microns Spot size selection Laser Energy Energy Level Selection Laser Shot Frequency Hz Laser Pulse Repetition rate selection Continuous Burst Laser Pulse Mode Selection Continuous laser fires until stop is pressed and Burst allows a fixed number of shots to be fired controlled by the shutter Use Shutter Delay With this option checked the user can designate the laser warm up and stabilization time CETAC recommends a minimum of 10 seconds Open Close Shutter After the shutter has opened the Open Shutter button will change to Close Shutter allowing the user to toggle the shutter open and closed Start Laser Initiates laser operations according to the specified energy and shot frequen
6. Cooler Power Supply The cooler power supply provides power for the laser and circulates cooled water through the laser head Host Computer The system is controlled by a personal computer In most cases the supplied PC should be used although another computer can be used if required The host computer runs the DigiLaz G2 software which controls sample positioning laser firing camera operation sample cell illumination and gas flow 10 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 1 Introduction Top cover Laser and Optical Components are enclosed Beneath the Cover Sample Cell Figure 1 3 Major Parts of the Laser Cabinet Shown with HelEx Cell Sample Cell Translation Stage Figure 1 4 Major Parts of the Laser Cabinet Standard Cell Hardware interlocks and other safety features are included in the laser and power supply modules These interlocks monitor the status of the entire ablation system and will ensure that all safety contacts are closed and the hookups are correct before the laser can be operated The laser will immediately switch off should any interlock be opened Laser Ablation System The LSX 213 G2 employs a specially designed Nd YAG laser frequency quintupled to the ultraviolet wavelength of 213 nm The LSX 266 uses a Nd YAG laser frequency quadrupled to the ultraviolet wavelength of 266 nm This laser provides a uniform energy profile flat top profile across all
7. Single Line Scan Draw Measure Manual Laser Control Launch Sequence Editor Start Sequence Stop Sequence Video Mode Method Selection A Drawn Method Parameters S l Spot Size Microns f Laser Energy 100 Is Laser Shot Frequency Hz 20 v Shutter Delay s Scan Rate microns s Axis Controls It f m w c Focus jS SLs 7nnm 79 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 4 Software Methods C Segmented Line Scan Description Segmented line scan allows the laser to follow features such as the curve of an otolith or any other irregular feature Each segmented line consists of a number of points which can all be edited Parameters Energy shot frequency scan rate and spot size can all be set The scan rate expressed in m sec automatically calculates the time per scan and the distance travelled How to Draw Select the Segmented Line Scan method Segmented Line Scan Method Selection Click anywhere on the sample image to set the start point Click again to end a segment and begin the next one Cancel Sample Mark Last Segment gt Edit Point Delete Sample Delete Last Sample Malsta All Carnnlaer 80 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 4 Software Methods xX EL General Operations Gas Managemert Video Operations Lighting Controls Camera Controls Triggering A Target
8. exposure times result in a brighter image To manually adjust this control uncheck the Auto box If Auto is enabled the gain will be set automatically and you will not have control over the brightness of the image Auto Reference and Auto Max fine tune how the automatic gain and exposure settings are calculated Higher numbers result in a brighter image 63 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 3 Using the DigiLaz G2 Software Color Device Properties DFx ALAUO2 E 73088 mmm White Balance Auto OnePush White Balance Blue x e G Saturation K pas H4 E ES E White Balance Red Figure 3 32 Color Tab Once the image is clear adjust the colors to best see the features of interest Hue shifts all of the colors Saturation affects the vividness of the colors TIP To see variations in the composition of the sample it often helps to raise saturation to near its maximum White Balance adjusts for the color cast of the image Setting the white balance with the OnePush button against a white image can allow colors to be more defined The Auto setting assumes something in the camera view is white and will attempt to correct the color to show this This can result in washed out images that appear dull and gray Don t forget that adjusting the polarizer angle can affect the colors too pU SSS SSS SSS aa Navigating With the Sample Map You can create a sample map to show the entire sur
9. gg Laser always on during sequence 397 Seconds EE run Run Sequence Go to Selection Total Sequence Run Time Figure 3 39 DigiLaz G2 Sequence Editor Sequence menu Sequence Operations In this group a sequence can be initiated or terminated You can run the all of the methods in the sequence or only a portion of the sequence Sample Selection This group contains the Go to Selection button When this button is clicked the stage will move to the start point of the highlighted row in the sequence This feature makes it easy to locate a drawn graphic on the sample image Laser Activity Use these options to turn the laser off between samples to prevent wasted shots over time If the Shut down laser between samples option is chosen an automatic 10 second pause will be enforced between each sample If this feature is used however it is recommended that at least a 20 second or longer Pause Between Samples be used to allow adequate time for warm up and stabilization of the laser CETAC recommends allowing the laser to continue firing between samples to maintain temperature and stability Total Sequence Run Time Displays the total time required to complete the entire sequence 73 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 3 Using the DigiLaz G2 Software Sequence Editing Tips Simple translation of an entire sequence can be accomplished visually by using the Transition Sequence feature page 70 More comple
10. 9 mL min an Online Helium 2 0 mL min gt Auto Flow Off 10 sec Set Flow s Bypass Additional 0 mL min s ontrols Sas Management Ctata Di i je Time Figure 3 12 Gas Management Tab Helium Flow Control LSX 213 G2 only This section allows a value to be designated for the desired gas flow rates A value can be manually entered in the mL min box or the scroll bars can be used to assign one After the desired setting is chosen click Set Helium Flow to push the flow settings to the laser This value can be changed at any time during a sequence run with immediate signal feedback for enhanced method optimization Any recorded flow in the sequence editor will have to be edited Gas Management State Purge Time amp Cancel Purge This section allows control of the on board valves used to direct carrier gas flows In the Online state the carrier gas helium for the LSX 213 G2 flows through the sample cell and mixes with Argon flow at the valves before exiting through the Sample Out port to the ICP or ICP MS The Bypass state is activated anytime a safety interlock is opened This state is used to divert gas flows coming from the sample cell away from the host instrument out to the vent This allows the sample cell to be opened for sample changes without extinguishing the plasma See Figure 3 14 As soon as the interlock is closed the valves switch to Purge mode This allows the sample cell to be purged and rem
11. Connect only as directed by Teledyne CETAC Technologies Due to variations in the design of the electronics cables might not be interchangeable between ICP instruments or between different laser ablation systems Improper connection may cause equipment damage to the laser ablation system or to the ICP 26 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 2 Using the Laser Ablation System Gas Connections The LSX 213 G2 uses both argon and helium during operation The LSX 266 only uses argon carrier gas One interface kit is supplied with the system For other interface kits please contact your local CETAC representative Argon Argon is used as a carrier LSX 266 or make up LSX 213 G2 gas The argon can be supplied from the nebulizer gas from the host ICP using the fittings and tubing provided The argon supply is connected to the CARRIER GAS port on the back of the laser cabinet If an additional argon mass flow controller MFC is installed use the provided fittings to connect 1 8 inch tubing from a separate argon source to the push fitting labeled ARGON on the back of the laser cabinet Helium LSX 213 G2 For the helium connection use the supplied fittings to connect 1 8 tubing from the source to the push fitting labeled HELIUM on the rear of the laser cabinet The vent port may be left open to atmosphere unless very hazardous material is being used which would require venting the residual sa
12. Duane cete o pe Dinos adnate 97 Periodic Checks of the Cooling System ees 97 Filling th Gooler PoWer SuDDIy isutia etii iare iine 98 Draining the Cooler Power Supply eterna 99 De lIonizing Cartridge Maintenance Inline Filter 99 Opening the Top GOVOPa dtu Minis eu Mix uM M tL Ss 101 Cleaning the Sample Cell WIBSQOMWSmeenesiuetuig acteur ttes Reunion ans 102 Replacing the Sample Cell Window senes 102 Replacing Internal Tublng uicit mre cte eere rcu 103 6 Troubleshooting the Laser Ablation System 107 Power System Problems aet hie oett ht inh hire deest 108 USOS nostis adi aded ORE OG EE ERA A DU ECOL HC AUR RADAR UNUS 108 Interface Problemi usn ote ote tuc CL i 110 RS 232 Cable Problems LSX 266 Only ss 110 Control Cable Problems terere terne tinet noia 110 Software Configuration Problems een 110 Sgrety InterlockPEODIG RIS dics nob e aco e onte E boit direi nt 111 Laser cabet InterloC RS odio tud Tt i pe ht e a e dd 111 Laser Controller InterioCRs ssim tete det c reis dens 113 Laser ProDle mS m SSR A 1 114 INOS Cl OUIDUE iia tssi uta saat aya area EM DI E Sadie 114 LOW LOSOr EITICIOH CVs acres e ee are renati A ceti e eu 115 Carrier Gas SVSLOITL scidit dri IRE R 115 Carrier Gas JOT the DSX 213 GZ T sumo pH U
13. Edit z Segmented Line Scan 9 Draw Measure Manual Laser Control Launch Sequence Editor Start Sequence Stop Sequence Video Mode Method Setection BOE Drawn Method Parameters va Spot Size Microns 40 v ertet Laser Shot Frequency Hz 2 Shutter Delay s 10 E Increment 66 39 microns 81 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 4 Software Methods Raster Line Description In the raster line the laser follows a line of discreet spots By drawing a line on the sample image to indicate the area of interest the software automatically calculates the number of spots which will fit on the space provided given the Spot size and distance between spots Parameters Enter the number of shots per spot and the spot size just as in the spot analysis but this method allows the distance between spots to determine the method pattern Energy and shot frequency may also be set The number of spots and time of analysis are automatically calculated How to Draw 1 Select the Raster Line method i Raster Line Method Selectian 2 Click anywhere on the sample image to set the start point 3 Click again to set the end point Example ue e S wD re a E General Operatiors Gas Managemert Video Operatiors Lighting Controls Camera Controls Triggering Toggle Cross Hairs V Toggle Lines Cross Hair Color Method Color c Dd L we x Sn
14. Managemert Video Operations Lighting Controls Camera Controls Triggering Toggle Cross Hairs V Toggle Lines E Method Color S D Ll X Snap Shot Record Stop Create Sample Map Cancel Sample Mapping Cross Hairs Lines y ia N E P Drawn Method Parameters EN m t fp hs n a x l Spot Size Microns FE Y bh gt Genk a i gt te lt 1 r l Laser Energy 100 E i 3 Laser Shot Frequency Hz EM w Shutter Delay s Burst Count Axis Controls 78 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 4 Software Methods Line Scan Description Single line scan allows the laser to move at a set rate across a sample in a straight line Parameters Energy shot frequency scan rate and spot size can all be set The scan rate expressed in um sec automatically calculates the time per scan and the X and Y distances traveled How to Draw l Selectthe Single Line Scan method io an Single Line Scan Method Selection 2 Click anywhere on the sample image to set the start point 3 Click again to set the end point Multiple repetitions of the line scan delay between scans and gas blank can all be set in the sequence editor to allow optimal integration with ICP software aam WHA DigiLaz G cos e holt x E General Operations Gas Managemert Video Operations Lighting Controls Camera Control Triggering 7 Target Edit Ne z
15. Q switched Nd YAG laser 266 nm Spot size range 10 200 um with standard aperture custom apertures are available gt 9 mJ pulse laser energy computer controlled Laser output energy is adjustable from 0 100 13 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 1 Introduction gt gt gt gt Flat top laser beam energy profile Laser pulse width lt 6 nsec typical Laser repetition rate 1 20 Hz Step resolution 0 25 um X Y axes 1 25 um Z axis Viewing Optics and Video System gt V vV ON WV Independent software controlled transmitted and ring lighting The LSX 213 G2 is also equipped with coaxial reflected lighting Computer controlled focusing 2 5 32 5X optical zoom range Computer controlled rotating polarizer for mineral grain viewing Thin section holder for petrographic slides and other transparent samples standard cell only Real time image acquisition Sampling System Standard cell is approximately 50 mm diameter by 50 mm high Multiple cell types available to suit nearly any sample type Quick release sample stage for easy sample exchange Automated valve system switches between cell purge bypass and online modes Ablation cell with removable quartz window for easy cleaning or replacement Computer Hardware and Software Y Y ON V WV Y V VV WV The DigiLaz G2 software controls all laser functions and runs ona dedicated PC which is
16. TOW CO DOW eon ase ace pen e ba ea oid actos ple e sc E 83 EXUIMDIG o b DU sd cce A E I ME 63 icr T H 84 DeSCEIDLION assu teh nied canes D ARRA CHEER R EDDA FUMER ONE HR ZEE RERO I pee 64 PFOFOBIOPOFES atasamatuie atate neben dne dalu iesus et cat A tenets 64 HOWTO DAL ET 64 EOM DIEN is UE UD M M UN ESTE 65 High Resolution Rastel icu ettet reti a S E tU DE cH Scc EU 86 DOSCEIDLIDI sienne p E RUD PR dd AERE EATON 66 FUPFORIIOLOT S Ae bor tcc kon d Al cO Da IM E UD ALI cL DDR OU 66 HOW tO DEGW xts5sucuctsautadiiutea uua xt e Sii nca Er 66 M ltisEIne SCdEs einen d eerta torn n etc ats cA rs EO Std os Mtr 89 DCS CHIP CON rin EDDIE FER ainmieaieae 89 PUT OIIOPOLS astitit ere oiu etter neice een rrer nar er vere er ted s eter rm ay 89 HOWTO TITY isdem cata obs acta dodo E MM 89 EOM DIE uc dass ERT Tee OS AE Ea EE 89 Depth PEODIBS SS coetu not ene Onna Montane M EU 91 PUF NBOFEPESE ect tM IM IM MEDI EM MM MEME 91 Is e Ero di e 91 EXAMP LP 92 CETAC LSX 213 G2 LSX 266 Operator s Manual Contents M ltiple Method Drawing soit troie a ema Een mtr rere tre needs 93 Segu ence Editor aonet n 94 5 Maintaining the Laser Ablation System eese 95 Safety Systems Maintenance Schedule sss 95 Satety 5ystem InspectI E aieo ttr orta x het creen rette teda 96 Goolmg System Malntenafi Ce diee eiit sie cii
17. The coolant flow is monitored by a magnetic switch that is located in the coolant loop If a coolant flow fault occurs the coolant flow has been stopped by an obstruction in the cooling loop or coolant hoses or the circulating pump has stopped due to a pump motor failure or blown fuses To test check for turbulence in the coolant reservoir If the coolant in the reservoir is not moving there is a blockage in the cooling loop that must be cleared drain the cooling system as described in Filling the Cooler Power Supply on page 98 If there are air bubbles in the coolant lines there could be a coolant flow fault A laser fault will appear on the power supply and a coolant fault will appear in the software To correct the problem turn the power supply key on for a few minutes and then turn it off Repeat this a couple of times until no air bubbles exist The fault indicators should disappear Remote Interlock If the remote interlock connection is broken by a bad cable or a fault in the interlock loop the laser cannot be operated Coolant temperature The coolant temperature interlock ensures that the coolant temperature does not exceed an acceptable level The coolant temperature sensor is located on the heat exchanger inside the cooling unit and will open if water temperature exceeds 150 F 65 C 113 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 6 Troubleshooting the Laser Ablation System EE Laser Problem
18. This computer may also control the analytical instrument Hz Hertz or cycles per second Unit of frequency ICP OES Inductively coupled plasma optical emission spectrometer OES is used as a synonym for AES atomic emission spectrometer ICP ICP MS Inductively Coupled Plasma Inductively Coupled Plasma Mass Spectrometer ICP Software The measurement automation software on the host computer which controls the analytical instrument ICP in this context can refer to both Instrument Control Program and Inductively Coupled Plasma spectroscopy Examples of ICP software include QTegra WinLab32 Quantum and MassHunter ID Inside Diameter LASER Light Amplification by Stimulated Emission of Radiation Laser head Component that generates the laser beam LED Light Emitting Diode 133 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 8 Glossary Method A single laser ablation pattern such as a point line or raster Nd YAG Refers to the Neodymium doped Yttrium Aluminum Garnet Nd Y3Als5012 crystal lasing medium used by the laser Pulse Repetition Rate The number of laser pulses per second expressed as Hz same as frequency Raster Moving the X Y Z stage to allow ablation of a series of spots in a line or area Sample cell Component that contains the sample for laser ablation Carrier gas flowing through the sample sell carries the ablated material to the analytical instrument Scan Moving the X
19. Video Operations tab select Create Sample Map Video Operations Lighting Control Camera Caontrals Triggering vV Toggle Lines ENS E Method Calor b Snap Shot Record Stop mm sample Cancel Sample Mapping Lines Figure 3 33 Opening the Sample Map Creator Window sample Map Creator File Operations Sample Map Operations Wid th 8 mms Cell Center 0 0 Height 12 Start Sample Map Abort Load Image Number of Images Origin Placement Mapping Controls Figure 3 34 Sample Map Creator Window In Sample Map Operations tab of the Sample Map Creator select the origin The origin will be the center of the sample map If the sample is centered in the cell choose Cell Center 0 0 otherwise choose Current Location Set the width and height of the sample map Tip Record these values You will need to enter this width and height when you re load the sample map Click Start Sample Map If you want to cancel the sample mapping process click Abort in the Sample Map Creator or click Cancel Sample Mapping in the main window which will also close the Sample Map Creator window 65 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 3 Using the DigiLaz G2 Software The completed sample map will appear in the Sample Map Creator Mapping Contr r Sample Map Creator eie Fite Operations Sample Map Operation wa wd 8 Current Location B Height 4 Start Sampie Map Abort load I
20. Y Z stage for continuous ablation over a line or area Sequence A set of methods which have been defined and stored together A sequence can be saved loaded exported or edited VAC Volts Alternating Current VDC Volts Direct Current Viewport The area in the DigiLaz G2 software which displays an image of the sample X axis The left and right axis of the X Y Z translation stage Y axis The fore and aft axis of the X Y Z translation stage Z axis The up and down axis of the X Y Z translation stage 134 auto gain 63 avertissements 129 avis Canadien 131 brightness 63 bulk analysis 39 burst 50 bypass state 53 camera controls 61 62 carrier gas 115 characteristics electrical 122 laser 13 cleaning 127 sample cell window 102 contamination 41 116 cooling system maintenance 97 defined presets 61 defocus 48 de ionizing cartridge 99 depth profiling 40 dimensions 15 disposal 131 earthing 25 electrical characteristics 122 emergency stop 36 energy calibration 76 exposure 63 FCC notice 130 focus 47 48 fuses 108 gain 63 gamma 63 gas blank 74 gas management 53 gradient profiling 40 grounding 25 Hardware Selection Tool 45 help online 76 high resolution raster 86 humidity 121 127 ICES 003 131 ICP software definition 133 inspection safety 96 interference 130 Index interlocks laser controller 113 location 111 status indicator 28 laser characteristics 13
21. and choose Edit Point from the menu Cancel Sample Mark Last Seqment pe Point Delete Sample Delete Last Sample Delete All Samples Transition Sequence Move the mouse to the new location and left click Only graphic positions x y can be edited in the main window Parameters for drawn graphics must be edited using the Sequence Editor Setting Method Parameters For each method a list of parameters specific to that method will appear in the Drawn Method Parameters section of the main window These parameters will be assigned to any new graphic drawn on the screen for that method However once a method graphic is drawn parameters for that method can only be changed in the Sequence Editor Notice that the Scanning and Multi Line Scan methods are similar to the Single Line Scan method but include an additional box for designating the space between the lines The Multi Line Scan method also includes a box to set the number of parallel lines to draw Moving a Sequence A sequence can be moved to a new position This is useful when the sample has been removed then replaced in a slightly different position or when a standard sequence needs to be aligned to different samples To move a sequence Load the sequence if necessary Note the location of the first point in the sequence labeled 1 on the display Right click anywhere on the display and select Transition Sequence Click on the new location for the first poin
22. and loose clothing away from the moving parts 2 ABRASION HAZARD Keep fingers hair and loose clothing away from the moving parts Power Cord Requirements and Safety Maintenance The power cord set supplied with your instrument meets the requirements of the country where you purchased the instrument If you need a different power cord contact Teledyne CETAC Technologies The operator should check the power signal supply cord condition The equipment should not be operated if the mains inlet is cracked or broken Any 126 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 7 Safety and Regulatory Information obvious damage to the case from a drop or fall should be checked by service personnel for loose or damaged parts See individual parts lists for approved replacement parts Mains Disconnect Power mains disconnect requires unplugging two power sources the desktop brick transformer and the cooler laser power supply Unplug the power cord at the power supply or at the wall outlet Ensure the power cords are easily accessible and removable in the event of an emergency which requires immediate disconnection SHOCK HAZARD Ensure that the power cords are disconnected before removing any covers Cleaning Instructions For additional cleaning information see cleaning in the index To clean the exterior surfaces of the instrument complete the following steps l Shut down and unplug the instrument 2 Wipe
23. be prevented from moving during shipping If the original shipping materials are not available contact Teledyne CETAC Technologies for advice Usethe original packing materials If the original shipping materials are not available place a generous amount of shock absorbing material around the instrument and place it in a box that does not allow movement during shipping Seal the box securely Contact Teledyne CETAC Technologies before shipping the product Pre pay all shipping expenses including adequate insurance Write the following information on a tag and attach it to the product Name and address of the owner Product model number and serial number Description of service required or failure indications Markthe shipping container as FRAGILE gt Inall correspondence refer to the instrument by model name or number and full serial number Donotreturn products which are contaminated by radioactive materials infectious agents or other materials constituting health hazards to Teledyne CETAC Technologies employees Product Warranty Statement NOTE Contact Teledyne CETAC Technologies or refer to the warranty card which came with your product for the exact terms of your warranty The following copy is provided for your convenience but warranty terms may be different for your purchase or may have changed after this manual was published TELEDYNE CETAC TECHNOLOGIES warrants that for 1 one year from th
24. both the power supply and the cooling system Allow the cooler power supply to run for about an hour to thermally stabilize Turn on the host computer 29 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 2 Using the Laser Ablation System NOTE Steps 2 to 4 must be performed in order If the steps are not performed in the correct sequence the software will not detect the interlocks as enabled and the laser will not initialize 5 Start the DigiLaz G2 software DigiLaz G2 6 Loadasample Loading a Sample Standard Sample Cell 1 Press the Load button in the software 2 Lower the shield Using both hands gently press down on the arms at the side of the shield It should move easily Figure 2 11 Lowering the Shield 3 Pull out the translation stage The translation stage is held in place by magnets While still holding the arms of the shield slide the translation stage forward to release it 30 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 2 Using the Laser Ablation System Figure 2 12 Pulling Out the Translation Stage 4 Remove the sample cell cover Twist the sample cell cover counter clockwise and lift Figure 2 13 Removing the Sample Cell 5 Load the sample into the sample cell Place the sample such as a 27 mm x 46 mm petrographic slide or a NIST 612 glass standard for system optimization on the sample holder 31 CETAC LSX 213 G2 LSX 266 Operato
25. ere ren ee Tee eee ee ere mee 72 SEGUENTE C 73 CETAC LSX 213 G2 LSX 266 Operator s Manual Contents seducen EnO MER o ct NEN E 74 Triggering and EHBITIg eei ieee abelian ie anaaenld kia 74 GOS BUG c I een 74 Pause Between Samples ccc aa M I E 74 POCTOU tut CM MI MELLE E 74 EXOIBBDISS une disc ir cL UCM Mu MEM EUM eee M ET 74 SeryiceCalibeation T0018 1 dt ect unti Ed daft sod eese rcp ne ouces 76 Helps IDE D RE Eu S LH ue 76 Software Methods eerie enero Peter uberior cinia Fee er o cr Pet 77 SDOUSCAIL assai na es tree eo eee eee ee ee 77 TOS CI OCI OV esa aia ca ecu E E RT RR VERD UE 77 FOE E a T A N T 77 HOWTO D OW E E A E 77 Example nnise a isl AL A I A El AD El S EE 78 EB SC dIhassteicuuncb ete sre du ee re IL uiia 79 Deser iPUON MED EU P czas tes tice pana tetas luteal sntestiaat atsccnosuavuatasiuiuaiaten tarennelsnasneniaies 79 TION CET Sarat acca UIN MEME 79 HOW TOD Waseca ceed stas ur eee ate Aaah usto a ue 79 EXAMP NH T AA TN 79 Sepmiented Line SCAN esis cinch autistic altace Mee aie ea vette a 80 BESC PUO Rt 80 FOODIE OT S etat uiii E EOT 80 HOWTO DTO W Mm neee nE OANE A R N OE R 80 qe C M 61 Raster LInetossodimei eR aceti ou cU de UD A UM EU LAU AM 82 TOS CVI DUO CHR 82 PFUFOIIOTOLS uitae bett bim uen 62 HOWTO JI OW airu a A A A UM 82 DOMPETE O AOO eT 82 SCANN 2 DO PTT 83 IB e qae E A T E HEY 83 POE IQ ELOT S RR 83
26. loaded laser operation and sample positioning parameters defined the system is ready to begin operation under computer control until the programmed sampling procedure is completed These instructions assume that you have installed the DigiLaz G2 software as described in the next chapter Do not start the laser ablation system unless the sample cell and shield are in the operating position the top cover of the laser cabinet is completely closed and all covers and safety interlocks are in place and operating DANGER INVISIBLE LASER RADIATION The laser ablation system is a Class laser system with interlocks and guarding It uses a Class IV Nd YAG laser The output beam is by definition a safety and fire hazard Precautions must be taken during use and maintenance to prevent accidental exposure to direct or reflected radiation from the laser beam To start the laser ablation system complete the following steps Check that the sample cell is in place the shield is raised and that the top cover of the laser ablation system is completely closed Turn the main power on The main power switch is located on the back panel of the laser cabinet Turn the cooler power supply on Make sure that the emergency stop button large round button on the power supply handheld controller is disengaged turn counter clockwise to release the button The power keyswitch is located on the front of the laser power supply The switch controls
27. of E I du Pont de Nemours and Company Swagelok is a registered trademark of Swagelok Company KIMWIPES is a registered trademark and KIMTECH SCIENCE is a trademark of Kimberly Clark Worldwide Inc All other marks are the property of their respective owners 1 Contents PACEOCUCHION RN TT ESEE AAEE 9 QI Igi pe estsece 9 Laser Abla Uon PEOCESS cdita ui Mix psec ecg en cece tU EMILIA Ri 10 Laser Ablation System Components essent nnns 10 LASET ADIAUON 3 SEO eirutuemiptemddituriatue inne stes tit Miet entr atrnt ens 11 L ser Lead and OPCS conim at mn inimi DM EE 12 Egu pment Suppe d orinn eai EEOAE NOE 12 System Characteristics amonia aAa A aaa 13 Loxa IIU T LUST antec ONE 13 BY 200 O ub E E E A EN 13 Viewing Optics and Video System ertet ntn nnns 14 Sampung Roy Ac meen nn utu at OD DRM MP 14 Computer Hardware and Software eerta anten 14 System CharFacteFISLICS uma tcr lan eto Veg EE aba bs aun a qat 15 Qr C X ere 15 EV CO UGS nce oe aac E RCM M ME 15 Who Should Use This Product Operator Qualifications 15 Where to Go for More Information sese 15 Regulatory and Safety Information reete 16 Additional Information from Teledyne CETAC Technologies 16 Feed EUNE aeterni nta Ed 16 P
28. operating conditions are met you can load samples and perform analysis with the laser ablation system This chapter explains how to create the proper operating conditions for using the laser ablation system It also explains laser safety precautions how to prepare and load the samples start and shut down the laser ablation system analysis procedures and initial operating parameters SSS aa Establishing Optimal Operating Conditions The laser ablation system operates reliably even under less than ideal conditions It is not however indestructible Malfunction or damage can occur if specific operating conditions are not met Meeting these conditions requires that you create the proper lab environment replace laser ablation system components that wear out under normal use and purchase the appropriate supplies for use with the laser ablation system The following sections explain how to meet these conditions NOTE Damage or malfunction that results from unsatisfactory operating conditions may constitute misuse and abuse and will be excluded from warranty coverage 19 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 2 Using the Laser Ablation System Creating the Lab Environment To create satisfactory operating conditions in your lab environment follow these guidelines gt Operate the laser ablation system in a conventional lab environment where the temperature is 50 85 F 10 30 C the humidity is 20 7
29. safety notices 124 troubleshooting 114 laser controls 49 LED See light lifting instructions 22 light status 28 lighting controls 60 line raster 82 line scan 79 mains disconnect 127 maintenance schedule 95 mapping 39 media 60 method parameters 70 methods 77 drawing 68 moving a sequence 70 multi line scan 89 online state 53 optimizing 38 pause between samples 74 pointer modes 51 polarizer 48 pollution degree 121 powder samples 42 power requirements 122 power cord 126 power off procedure 36 power on procedure 29 pre ablation 41 prism 102 purge state 53 purge time 53 recording 60 regulatory notices 121 return procedure 117 safety information 121 135 CETAC LSX 213 G2 LSX 266 Operator s Manual sample powder 42 size 41 sample cell cleaning 102 loading 31 33 size 41 window replacement 102 sample map 64 segmented line scan 80 sequence editor 71 94 service 117 settings camera 61 Shield raising 32 shipping 99 117 shutter delay 50 Slide petrographic 41 spot scan 77 stage controls 47 status light 28 step size 47 storage 37 surface preparation 41 sweep gas See carrier gas symbols warning 124 128 target mode 51 temperature operating 121 recommended 20 timing 74 transition sequence 70 trigger delay 74 triggering 74 trip hazard 22 tubing replacement 103 ventilation requirements 17 video operations 60 video settings 6
30. spot sizes and yields a flat bottomed crater on the sample The aperture system uses a motor driven ceramic wheel with several positions that are selected within the software The laser can be operated at a high repetition rate of up to 20 Hz for increased sampling rate and better ICP MS sensitivity 11 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 1 Introduction The sample cell is mounted on a computer controlled X Y Z translation stage The translation stage provides X Y positioning control for laser targeting on the sample The Z axis of the translation stage is used to focus the laser via the CCD camera viewing system The sample image is viewed directly in the DigiLaz G2 software see Figure 1 5 Since air is unavoidably admitted into the sample cell when changing samples removing the air from the carrier gas flow path prior to switching back to the ICP MS is required to prevent plasma collapse To purge the sample cell and prevent plasma collapse the laser ablation system is equipped with electrically actuated valves to direct the carrier gas flow to either the ICP MS or to a purge vent The CCD camera microscope system provides a means of visual identification of the sample areas of interest and also for laser focusing A digital crosshair provides a targeting mechanism for the sample positioning at the point of laser impact DARA Doc r x E tio Gas Main Toolbar Drawn Method Parameters
31. supplied with the instrument Compatible with the Microsoft Windows 7 operating system Communication via serial port LSX 266 or USB LSX 213 G2 On screen display of safety interlock and laser status External trigger to synchronize ablation with host instrument Built in laser ablation methods including multi spot analysis line scans and raster segmented line scanning area scan and raster and advanced depth profiling Method sequence saving export and loading with sample image capture and export Spot size and feature measurement functions Single shot or automated repetition burst or continuous mode Computer adjustable spot size using aperture masking of the laser beam Automated sample motion control CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 1 Introduction System Characteristics Laser cabinet dimensions 73 x 46 x 53 cm depth x width x height gt Cooler power supply dimensions 45 x 13 x 36 cm depth x width x height Cartdimensions 112 x 63 x 58 cm depth x width x height of work surface Weight approximately 68 kg 150 pounds for laser cabinet with power supply Power requirement 100 250 VAC 10 Class I enclosure with safety interlocks and guarding Independent programmable laser power supply module VV V WV Closed loop water cooling system with integrated DI cartridge Options gt Custom designed sample cells and apertures are available Please contact your local Teledyne CE
32. the LSX 266 The carrier gas for the LSX 266 is typically argon only A helium mass flow controller is not included in the LSX 266 The nebulizer gas port on the ICP ICP MS is connected to the CARRIER GAS connector in the rear of the laser system E 7 Contamination Problems Sample Surface Contamination Surface contamination may result from cutting grinding or polishing the sample surface In most cases surface preparation should be avoided Where surface contamination already exists consider using pre ablation to remove surface contamination For more information see Sample Preparation on page 41 Cross Contamination Cross contamination can occur if many different sample types are used A complete tubing change may be required if the system is being used continuously and with different samples The operator must be aware of the background materials to prevent any cross contamination from occurring 116 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 6 Troubleshooting the Laser Ablation System UE DUAE M S Returning the Product to CETAC for Service Refer to the following information if you need to return the product to Teledyne CETAC Technologies for service Shipping the Product Follow these guidelines when shipping the product Drainthe coolant as described on page 99 Serious damage may result if water remains in the system and freezes during shipment Securethe sample stage The stage must
33. the instrument exterior surfaces only using a towel dampened with a lab grade cleaning agent 3 Repeat step 2 using a towel dampened with clear water 4 Dry the instrument exterior using a dry towel SHOCK HAZARD Do not allow any liquid to enter the instrument cabinet other than as intended through the specified tubing or come into contact with any electrical components The instrument must be thoroughly dry before you reconnect power or turn the instrument on Operating Environment SHOCK HAZARD To reduce the risk of fire hazard and electrical shock do not expose to rain or humidity To reduce the risk of electrical shock do not open the cabinet All maintenance is to be performed by an Authorized CETAC Service Provider Protection provided by the equipment may be impaired if the equipment is used in a manner not specified by the manufacturer 127 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 7 Safety and Regulatory Information SHOCK HAZARD Equipment is not intended for wet locations Miscellaneous liquids in the equipment could cause hazardous conditions EXPLOSION HAZARD Do not operate in an explosive atmosphere Explanation of Caution and Warning Notices A Warning symbol marked on equipment This symbol means Attention Refer to the manual Refer to manual symbol marked on equipment This symbol means Read and understand the manual before operating or servicing this eq
34. the red fitting to the ICE450 Remove the vent fitting from the front of the reservoir and gently do not use air pressure greater than 0 35 bar 5 psi blow air into the corresponding red fitting at the laser head end of the coolant lines To prevent possible organic contamination of the coolant loop do not blow by mouth into the tube Continue to blow air until no more water is visible through the drain tube Then disconnect the red fitting from the ICE450 connect the blue fitting and repeat this process Next disconnect the blue fitting Finally reconnect the vent tube to the vent fitting on the reservoir and tip the ICE450 forward to cause the water to run to the front Following this detailed procedure will drain all the water from the system and prevent freezing damage during shipment De Ionizing Cartridge Maintenance Inline Filter The ICE450 cooler power supply can provide years of reliable performance given proper handling and some minor routine maintenance Visually inspect your ICE450 monthly for coolant leaks abnormal noises or damage to connectors and coolant ports The de ionization cartridge and coolant must be changed every six months Follow this procedure to change the deionized water 99 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 5 Maintaining the Laser Ablation System Before proceeding switch the cooler power supply OFF 0 and unplug the AC power cord 1l Drain the coolant from the sys
35. with the laser beam Remove the sample cell top and clean with dry compressed air If necessary wipe the window and cell clean with a dry lint free wipe such as a KIMTECH SCIENCE KIMWIPES Delicate Task Wiper Do not use any cleaning solution on the sample cell window or other optical components Solvents may damage the optical coating Figure 5 7 Location of Prism Replacing the Sample Cell Window If the window cannot be cleaned using this procedure a replacement window can be ordered directly from Teledyne CETAC Technologies The standard cell window is held in place against a sealing O ring by a flange Moderate hand tight torque is required for a good gas seal Take care to avoid breaking the window by overtightening 102 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 5 Maintaining the Laser Ablation System Tighten the flange a little at a time moving in a star pattern Replacing Internal Tubing In the event the sample gas valve assembly tubing becomes contaminated it must be replaced A spare parts kit can be obtained from CETAC In lieu of purchasing the kit the tubing can be changed with stock FEP lined Tygon tubing All tubing is 1 4 O D and 1 8 I D Symptoms that the tubing needs to be replaced can include the following Gas blan
36. 0 non condensing and the unit is not exposed to excessive flammable or corrosive materials Avoid rough handling Do not expose the laser ablation system to vibration or shock Protect the system from long term exposure to condensation corrosive materials solvent vapor standing liquids or liquid spills into the electrical equipment Do not operate inside an acid hood or glove box Exposures of this type can degrade the optics corrode and damage mechanical drive mechanisms as well as the electronics Observe the same general electrostatic discharge precautions as with any other integrated circuit electronic devices Low humidity environments especially when combined with static generating materials require maximum care Discharge static buildup and ground yourself to the laser cabinet before performing any maintenance Do not touch or short circuit bare contacts of any communications ports gt Avoid exposing the system to high levels of electromagnetic or radio frequency interference EMI RFI or radioactivity EMI RFI can cause erratic operation high levels of radioactivity may cause electronic and optical component failure and will prohibit factory repair if so contaminated Contact Teledyne CETAC Technologies for assistance if the system will be required to operate in a hostile environment 20 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 2 Using the Laser Ablation System Replacing Laser Ab
37. 152 36 152 36 1541 94 1541 94 Raster Line Line 2 43 89 350 69 1770 63 1796 11 Raster Line Figure 3 37 DigiLaz G2 Sequence Editor You can use the Sequence Editor to make precise adjustments to methods after they have been drawn on the screen Changes made in the Sequence Editor will automatically be updated on the sample image 71 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 3 Using the DigiLaz G2 Software When you need even more editing power it is easy to export the sequence to a Microsoft Excel spreadsheet edit it then import the sequence back into DigiLaz G2 NOTE DigiLaz G2 does not support importing Excel files with formulae To ensure proper import select the whole sheet copy it and paste back into the same sheet using the Paste as Values function in Excel before saving the edited sequence General The General tab includes two groups of commands File Operations and Print Operations File Operations This group contains buttons which allow you to create a new sequence open save a sequence and export import a sequence to and from Microsoft Excel When the Open Save Sequence or Export Import buttons are clicked a window will automatically open allowing you to Open Save or Export Import Sequences By default Sequences are stored in the Sequence folder located in the following path C ProgramData CETAC TechnologiesMDigiLaz G2 Sequences TIP For easy access you can add the Sequences
38. 2 warning symbols 124 128 warranty 117 WEEE notice 131 weight 15 zoom calibration 76 Manual Part Number 480202 Rev 1 Printed in USA 136
39. 70 80 Time s 10 20 30 40 In the example below with a Trigger Delay value of 30 seconds and a Gas Blank of 10 seconds the trigger signal will be sent 20 seconds after the laser has begun to fire If the Gas Blank value was set to 20 seconds in this example the signal would be sent 10 seconds after the laser has begun to fire MEER Shutter Gas Blank Trigger anpe Total Number of Again represented visually a timing map shows the trigger being activated 20 seconds after the ablation has begun Sequence Start Trigger Shutter Delay Gas Blank Ablation 10 20 30 40 50 60 70 80 90 Time s 75 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 3 Using the DigiLaz G2 Software SSS SSS SS SSS Service Calibration Tools Manual Laser Control 3 File b Launch Sequence Editor Create Sample Map X Cancel sample Mapping heo gt il Ssss A Perform Zoom Calibration 4 Exit i Launch Energy Calibration Tool Figure 3 40 Launching the Service Calibration Tool Two extra functions are only accessible from the Tools menu These are Energy Calibration and Zoom Calibration tools These functions are primarily used by a service engineer to recalibrate zoom steps and energy parameters As such these functions are described in another document only available from the Teledyne CETAC Technologies service department These tools should only be accessed on request of the CETAC service enginee
40. ATION AVOID EYE OR SKIN EXPOSURE TO DIRECT OR SCATTERED RADIATION AVERTISSEMENT RAYONNEMENT LASER VITER TOUTE EXPOSITION DES YEUX ET DE LA PEAU AU RAYONNEMENT DIRECT OU DIFFRACTE The cooling system located in the laser power supply is filled with DISTILLED WATER DO NOT fill with any other type of fluid The cooling system should contain a sufficient amount of distilled water The fault light will come on if the coolant is in interlock mode AVERTISSEMENT LE SYST ME DE REFROIDISSEMENT EST REMPLI D EAU DISTILL E EVITER LE GEL OU L APPAREIL SERA D FINITIVEMENT D T RIOR COOLING FAN OBSTRUCTION The laser power supply cooling fan should remain unobstructed at all times Do not operate the instrument if the cooling fan is blocked or obstructed in any manner Electromagnetic Interference FEDERAL COMMUNICATIONS COMMISSION FCC NOTICE This equipment has been tested and found to comply with the limits for a Class A digital device pursuant to Part 15 of the FCC Rules These limits are designed to provide reasonable protection against harmful interference in a commercial installation This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instructions may cause harmful interference to radio communications Operation of this equipment in a residential environmentis likely to cause harmful interference in which case th
41. Additional periodic maintenance tasks are required including replacement of the following laser ablation system components gt Coolant gt Coolant de ionizer cartridge gt Tubing Replacement gt Sample Cell Window ee ee ad Safety Systems Maintenance Schedule The laser ablation system is designed as a Class I laser product to contain all hazardous laser radiation during normal operation as required by the U S Department of Health and Human Services Normal operation of the laser ablation system shall not be allowed unless all safety systems are operational Periodic safety systems inspection and performance testing are mandatory to insure continuous laser radiation containment Verify the operation of all safety systems at least semi annually or whenever the system has or may have been transported subjected to damage or any other adverse conditions that could have affected safety systems operation 95 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 5 Maintaining the Laser Ablation System a OO nar Safety System Inspection EYE DAMAGE HAZARD Wear appropriate personal protective equipment to protect yourself from the laser beam until you have verified that all safety shields and interlocks are working properly Before proceeding read all of the safety notices see Safety and Regulatory Information on page 121 Tampering with or deactivating safety systems and or interlocks may resu
42. Color buttons open a palette to control the color of the lines Figure 3 25 Cross Hair Color a Automatic BEEEEEE BREE EEE ERE EEE EROS ES I IE ES E E3 C1 E C1 f m More Colors Figure 3 25 Cross Hair Color Palette Snap Shot Records a JPEG image of the viewport A Save As window will open allowing you to designate a file name and save location Record Stop Records an AVI video of the viewport A Save As window will open allowing you to designate a file name and save location Create Sample Map Creates a set of images over the designated region of the sample See Navigating With the Sample Map on page 64 Lighting Controls The Lighting Controls tab contains controls for the LED lighting You can increase or decrease the percentage of lighting used by clicking the or signs or by simply clicking an area within the percentage box n NOE DigiLaz G2 E General Operations Gas Managemert Video Operations Lighting Controls Camera Controls Triggering u s ae Synchronize Zoom Upper Lights MI io ga ERE 20 Eg Synchronize Zoom Lower Lights Main Cell Lighting Coaxial Lighting Zoom Illumination Synchronization Figure 3 26 Lighting Controls Tab Synchronizing the zoom to the lights increases the lighting percentage as the zoom percentage increases This makes it easier to view the sample at high zoom levels 60 CETAC LSX 213 G2 LSX 266 Oper
43. E DIE 116 Carrier Gas gor the 3X 200 esiti bte bm etit avert 116 Contamination Problems eei ini b d oc re d bri a o is 116 Sample Surface Contamination setae tnnt 116 Cross CORCOIBIAGCODO2a did rre roi raa aient eu eH asesor 116 Returning the Product to CETAC for Service sss 117 SID DING CHET OOUCE sid A den 117 Product Warranty Statement aa uic tat uer cie 117 Returned Product PFOCedUreSu a n RR RR d IR des 118 Returned Product Warranty Determination sess 119 7 Safety and Regulatory Information eese 121 Gabi ski 121 En ronmental Characteristics santi oit eat a et id 121 FOWEF Tedguiremoehtssc deus bacdbd stri bnc bU Da Mies 122 Inputand Output Connectors iei aide na dep vetet abri dio 122 FUSES D 123 Somalie c 124 LASTS A O O cues UERSUM UE DIE 124 U S Regulations Class I Laser Product e 124 CDRH Laser Product Regulations serere 124 Laser Safety Labels 2e a ai OR a T EAE 124 COOLA jk S note E MEM 126 CETAC LSX 213 G2 LSX 266 Operator s Manual Contents VOHCHO TID secus eb ni d hon DU EE bnt 126 Mechanicat HaZakus sa eun ERE e Ti ee tomi eMule 126 Power Cord Requirements and Safety Maintenance 126 Mans DVIS COMIC CU a ov tesa reda LE EE 127 Cleaning HVS CIN LOU au ba pa Rau
44. ES or ICP MS systems and is largely dependent on the desired level of sensitivity of the particular application The following general scheme can be used to perform accurate and precise quantitative analysis Select a set of homogeneous reference materials to be used for calibration standards and quality control check samples The standards and samples should be matrix matched as closely as possible using an internal standard to correct for differences The internal standard improves the precision greatly and should always be used when possible In the ICP OES or ICP MS software create a suitable quantitative analysis method For example use integration times or dwell times that will collect data for 1 2 minutes of sample ablation In the DigiLaz G2 software select one of the methods which samples a large area either grid of spots scanning area or line scan Set up the sample area and ablation rate so that ablation will begin 30 60 seconds before analysis begins The extra time allows the particle transport to stabilize Optimize the laser pulse rate carrier gas flow spot size and scanning rate to achieve the desired sensitivity and stability Typical settings for maximum stability and sensitivity in a line or area scan are Pulse Rate 20 Hz Carrier gas He flow 500 900 mL min optimized in concert with ICP nebulizer flow Spot size 100 200 um depending on sample size Scanning rate 10 20 um sec NOTE Often several
45. L CIRCUITS DEVICES 9 AND COMPONENTS OPERATING AT Ne DANGEROUS VOLTAGES CONTACT A SE CIRCUITS DEVICES D COMPONENTS CAN CAUSE SERIOUS INJURY OR AINFUL ELECTRICAL SHOCK PERATORS AND OTHER UNAUTHORIZED PERSONNE EVER OPEN THE MAIN C THE MAIN COVER OF THIS STRUMENT MUST ONLY BE OPENED BY TRAINED QUALIFIED OR APPROVED SERVICE ENGINEERS J gt T E j c E am m O TOF p gt I L MUST OVER D AVERTISSEMENT TOUT CONTACT AVEC LES HAUTES TENSIONS PEUT ENTRAINER LA MORT OU DES BLESSURES S VERES CE PANNEAU NE DOIT TRE ENLEVE QUE PAR UN R PARATEUR QUALIFI AVERTISSEMENT TOUT CONTACT AVEC LES HAUTES TENSIONS PEUT ENTRAINER LA MORT OU DES BLESSURES S VERES CE PANNEAU NE DOIT TRE ENLEVE QUE PAR UN R PARATEUR QUALIFI AVERTISSEMENT NE PAS GLISSER LA MAIN SOUS OU DERIERE LES ECRANS THERMIQUES DU FOUR GARDER LA PORTE D ACCES AU DEVANT DU BOITIER BIEN FERMEE POUR ASSURER LA PROTECTION CONTRE LES BRULURES AWARNING CONTACT WITH DANGEROUS VOLTAGES CAN CAUSE DEATH OR INJURY COVER TO BE REMOVED ONLY BY TRAINED SERVICE PERSONNEL AVERTISSEMENT TOUT CONTACT AVEC LES HAUTES TENSIONS PEUT EN
46. L file click Save Current Settings If you save the settings in the Presets directory the settings file will be added to the Defined Presets list after you restart the DigiLaz G2 software To load a camera settings file click Load Saved Setting 61 A W N HM CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 3 Using the DigiLaz G2 Software Adjusting the Camera Settings The camera settings help you to see the sample clearly as you set up an ablation sequence There is no right or wrong way to set up the image al DN Ae B DigiLaz G2 i General Operatiors Gas Managemert Video Operations Lighting Controls Camera Controls Co rj SN n Adjust Settings Save Current Settings Load Saved Setting Defined Presets Figure 3 29 Light and Camera Controls On the Camera Controls tab click Adjust Settings The Device Properties window will appear As you make adjustments click OK to see the effect of the changes The camera is turned off while the camera Device Properties window is open The Update and Apply buttons have no effect Initial Image Adjustments Sometimes the image will be much too bright or too dark at first A good sequence to get close to the right settings is Begin with a defined preset appropriate for your sample Zoom all the way out On the Camera Controls tab click Adjust Settings On the Exposure tab make sure all of the Auto boxes are checked then click OK Adjust the u
47. LSX 213 G2 LSX 266 Operator s Manual Chapter 5 Maintaining the Laser Ablation System Vent Carrier Gas In Sii i L Sample Out A Pressure Relief Valve Sample i Argon Figure 5 9 LSX 266 tubing diagram showing gas flow in the online state Figure 5 10 Location of Tubing and Valves LSX 213 G2 shown 105 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 5 Maintaining the Laser Ablation System This page is intentionally blank 106 Troubleshooting the Laser Ablation System In the event that the product does not function properly isolate the problem to determine if it originates in the host computer the analytical instrument ICP or the laser ablation system If you determine the problem is in the laser ablation system check the AC power transformer the communications interface the safety interlocks or the laser head to find the cause of the problem and resolve it This chapter explains how to troubleshoot minor problems If you cannot solve a problem using the steps given in this chapter contact Teledyne CETAC Technologies Customer Service and Support 1 800 369 2822 in the U S or 1 402 733 2829 outside of the U S or an authorized service representative 107 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 6 Troubleshooting the Laser Ablation System SSS SSS SSS SS ee Power System Problems If the laser ablation system is not responding power may n
48. Manual Contents Emergency Stops duae natn tix da C usu a apu 36 In Gase Of Electrical HaZ Eu iua en e ta doni 36 Normal ShutaoWnz iiti tm ae engin aaa cs tu 36 Storing the Laser Ablation System sesenta 37 Storing for Less Than One Week moaautuem c n rito neater arenas 37 Storing for a Few VW OORS taucastin in i un rne Ret neni DA dtu ER 9 Stormo for Over TWO MONS aito eo eint 37 Methods OP Analyses cia a cn tama a eee eet 38 Optimizing the Laser Ablation Parameters eere 38 BUKAN SIS osse SE LOS t M LM E 39 Fiementaltor Spatial Mapping sconto tae M tn EDO 39 Depth PIO fling sake tace tU aO n bolt rain doe dubius 40 Satnple oe 55 OF er 81K G 6 Mere RI RCRA EE et steel ene e 41 SIMPE OZO caveats csi em ncn o ep ads io E Let einen am alee 41 Sar ace CONUS Gusto BUDE DEM 41 TOW QIN CS ostii mati mitad vest cde ose eda tbi eni alie diio 42 3 Using the DigiLaz G2 Software eese nnns 43 Installing the DigiLaz G2 Software sesenta 44 Running the DigiLaz G2 Software for the First Time 44 Using the Stage Controls to View the Sample sss 47 MOCION GOBLUKOLS taste ta itia ma A RO CE RE RR cro 47 FOCUS CODI EOS corno MSIE UMEN MUI EE 48 LOOT aha eal X X S 48 Iren M 9 48 POIGPIZOE iaceat a tsm enu m eI M E DNA AT DUE ee UE 48 Tabs i
49. Map A previously created sample map can be re used at any time 1 Open the Sample Map Creator window by clicking Create Sample Map in the Video Operations tab 2 Inthe File Operations tab click Open Sample Map and open the desired sample map 3 OntheSample Map Operations tab click Load Image Ensure that the Width and Height are set to the values originally used to acquire the loaded sample map to maintain accurate navigation The sample map will now appear below the viewport in the software and can be used for macro navigation To use it click anywhere on the sample map to center the camera on that point Importing a Sample Map For the larger sample chambers for example the HelEx cell mapping the working area of the cell can take a very long time In this case itis preferable to import either a scanned image or a photograph of the samples in the chamber NOTE The Upper Left corner of the video image is the Front Right corner of the sample cell as viewed from the front l Choose two landmarks in the sample chamber one of which is the upper left corner of the sample map to be imported and the other is the lower right corner Move the stages to these positions and note the X Y coordinates 2 Open the Sample Map Creator window by clicking Create Sample Map in the Video Operations tab 3 IntheFile Operations tab click Import Sample Map and open the desired image sample Map Creator File Operations Sample Map
50. Mulki Lin Scan Method Selection Set the method parameters including the number of lines Click anywhere on the sample image to set the start point of the first line Click again to set the end point of the first line Once you have drawn the first line of a Multi Line Scan the additional lines are automatically created Each line is a separate Single Line Scan so you can edit the end points energy and other parameters Example 89 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 4 Software Methods DigiLaz G M E General Operations Gas Managemert Video Operations Lighting Controls Camera Controls Triggering e 7 4 Ld Target Edit E Multi Line Scan Draw Measure Manual Laser Control Launch Sequence Editor Start Sequence Stop Sequence Video Mode Method Setection Drawn Method Parameters 4 Spot Size Microns 40 Space Between Lines Microns Laser Energy 9 Laser Shot Frequency Hz Shutter Delay s Scan Rate microns s Number of Lines I tj era eis 90 n B 100 e 2 10 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 4 Software Methods SSS SSS SS Depth Profiling As the laser ablates material from a single point the bottom of the crater moves out of focus with respect to the laser beam This can lead to signal loss and inefficient ablation with deeper craters To compensate the Depth Prof
51. Nw TELEDYNE CETAC TECHNOLOGIES Everywhereyoulook 4 LSX 213 G2 and LSX 266 Laser Ablation Systems 1 CARAC TECHNOLOGIES nam TEhines inc Operator s Manual Manual Part Number 480202 Rev 1 COPYRIGHT 2006 2013 Teledyne CETAC Technologies 480202 Rev 1 September 2013 Teledyne CETAC Technologies authorizes its customers to reproduce transmit or store this document in its entirety including this page for the express purpose of installing operating or maintaining the product described herein Teledyne CETAC Technologies Customer Service amp Support 14306 Industrial Road Omaha Nebraska 68144 USA Phone 800 369 2822 USA only Phone 1 402 733 2829 Fax 1 402 733 1932 E mail custserv cetac com REVISIONS Teledyne CETAC Technologies strives to provide the scientific community with an unparalleled combination of effective technology and continuing value Modular upgrades for existing instruments will continue to be a prime consideration as designs progress Teledyne CETAC Technologies reserves the right to revise this document and or improve products described herein at any time without notice or obligation TRADEMARK ACKNOWLEDGEMENTS Windows is a registered trademark of Microsoft Corporation in the United States and other countries PharMed and Tygon are registered trademarks of Saint Gobain Performance Plastics DuPont and Teflon are trademarks or registered trademarks
52. Operations M E Save Sample Map Open Sample Map Clear Sample Map Import sample Map 4 Selectthe upper left and lower right landmarks in the imported image 67 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 3 Using the DigiLaz G2 Software The Coordinates tab will now be visible and active Enter the coordinates noted in step 1 On the Sample Map Operations tab click Load Image The Sample Map will now appear below the viewport in the software and can be used for macro navigation To use it click anywhere on the sample map to center the camera on that point Drawing and Editing Method Graphics Drawing a Method Graphic Click the General Operations tab General osi Gas Man Click Draw in the Pointer Mode group Target Edit iq Draw Measure esed asc Mode Set the method parameters The list of parameters depends on the method selected Drawn Method Parameters Spot Size Microns Space Between Spots Micro Laser Energy 9 Laser Shot Frequency Hz Shutter Delay s 10 a Burst Count 100 aj 68 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 3 Using the DigiLaz G2 Software Click on the sample image to define the starting point of the method Continue clicking to set intermediate or end points if required See Software Methods beginning on page 77 for details To mark the final segment for the Segmented Line Scan method right click and choose Mar
53. TAC Technologies representative for further information Safety Features gt The laser ablation system has built in safety interlocks to disable the laser whenever any shields or covers are not in their operating positions Another interlock on the laser power supply deactivates the laser if its cover is opened during operation The laser power supply is controlled by a key switch and the key can be removed only when the switch is in the OFF position to prevent unintended operation Who Should Use This Product Operator Qualifications The laser ablation system along with this book is intended for use by analytical chemists and lab technicians To use this product safely and effectively at least a beginning level of knowledge and experience about laser safety electrical electronic equipment operation and maintenance personal computers and ICP MS or ICP OES are required Laser safety certification for operators is not required but CETAC recommends that all users familiarize themselves with laser safety before using the laser ablation system a EEE EE Where to Go for More Information In addition to this manual you can refer to the following resources for citation material or for further information 15 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 1 Introduction Regulatory and Safety Information Safe Use of Lasers Z136 1 American National Standards Institute ANSI www ANSLorg Safe
54. TRAINER LA MORT OU DES BLESSURES S VERES CE PANNEAU NE DOIT TRE ENLEVE QUE PAR UN R PARATEUR QUALIFI AWARN CONTACT WITH DANGE VOLTAGES CAN CAU DEATH OR INJ TO BE REMOVE BY TRAINED S D ERSONNEL AVERTISSEMENT TOUT CONTACT AVEC LES HAUTES TENSIONS PEUT ENTRAINER LA MORT OU DES BLESSURES S VERES CE PANNEAU NE DOIT TRE ENLEVE QUE PAR UN R PARATEUR QUALIFI WARNING HIGH LEAKAGE CURRENT ENSURE PROPER GROUNDING AVERTISSEMENT COURANT DE FUITE LEV FORNIR UNE MISE LA TERRE EFFICACE 129 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 7 Safety and Regulatory Information AVERTISSEMENT SURFACES CHAUDES LAISSER LE COUVERCLE HERM TIQUEMENT FERM POUR ACC DER METTRE LA TEMP RATURE DU FOUR Z RO OUVRIR LE COUVERCLE ET LAISSER REFROIDIR 5 MINUTES AVANT DE TOUCHER LA VERRERIE OU TOUTE SURFACE M TALLIQUE INT RIEURE On Supply Off Supply AVERTISSEMENT POUR LA PROTECTION PERMANENTE CONTRE UN CHOC LECTRIQUE UNE BR LURE DES YEUX RADIATION UV OU DE LA PEAU LAISSER LE COUVERCLE HERM TIQUEMENT FERM LORSQUE L APPAREIL EST SOUS TENSION LAISSER REFROIDIR 5 MINUTES APPAREIL TEINT AVANT D ENLEVER LE COUVERCLE L UTILISATEUR DOIT LIRE ET VEILLER BIEN COMPRENDRE LE MANUEL UTILISATION AVANT D UTILISER L APPAREIL 4 WARNING LASER RADI
55. a homogeneous reference material such as the NIST 612 glass that is supplied in the sample cell Using the Single Line Scan laser ablation method set up a line across the sample surface using laser parameters that are similar to the types of analysis that you plan to perform For example if the samples will be analyzed using a scan rate of 10 um sec and a 100 um spot size use these parameters for optimization Set the helium flow to 600 mL min typical values 500 900 mL min LSX 213 G2 only All other laser parameters should remain the same between samples and tuning This includes spot size energy and pulse rate Optimize the ICP MS system and helium flow using typical hardware settings such as nebulizer gas ion optic voltages and torch interface parameters An ICP OES can be optimized in the same way Optimizing the viewing position nebulizer gas power and helium flow NOTES The steps noted above vary greatly depending upon the type of ICP MS or ICP OES Consult the appropriate operating manual for details on tuning and optimizing the instrument For more precise optimization use a homogenous reference material that is matrix matched to your samples 38 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 2 Using the Laser Ablation System Bulk Analysis Bulk quantitative analysis is a common application that is well suited to laser ablation sample introduction Bulk analysis can be performed on ICP O
56. ake sure that the emergency off button is not engaged the large red button on the front of the cooler power supply it should protrude about 1 cm If not turn it in the direction indicated by the arrows and it will pop out 3 Check the wall outlet The power supply may trip a GFI ground fault interrupt outlet in its normal operation as it discharges internal capacitors Make certain that the cooler power supply unit is not plugged into a GFI outlet If it is move the AC line cord to a standard outlet or have a qualified electrician switch the AC power outlet from GFI to a standard grounded outlet ETT Do not attempt to service the electronics in the laser cabinet There are no user serviceable parts inside the laser cabinet The rear panel of the cabinet should not be opened except by an authorized service representative Fuses The only fuses in the laser ablation system are in the laser cooler power supply The laser cabinet has no replaceable fuses The cooler power supply has one fuse which can be checked if it does not operate despite taking the 108 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 6 Troubleshooting the Laser Ablation System actions outlined above The fuse is accessed from the rear of the power supply and is held in place with a straight screwdriver cover Check and replace if necessary Contact CETAC Customer Service if there are any questions or if the unit requires factory service La
57. ap Shot Record Stop Create Sample Map Cancel Sample Mapping Cross Hairs Lines Y D A E E I E E Drawn Method Parameters E Y E LS Se T N 4 Spot Size Microns 40 Space Between Spots Microns Laser Energy 96 100 Ie Laser Shot Frequency Hz 20 Shutter Delay s Burst Count 100 fe Axis Controls ta n orem E384 Rf A 82 ui A U N CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 4 Software Methods Scanning Description Defines an area using the mouse This area is then divided up automatically given the distance between lines and the spot size Parameters Energy shot frequency and spot size may be set The number of lines distances and time of analysis depend on the area defined with the mouse and scan speed selected This scan can be repeated if selected with delays and blanks to synchronize samples with the ICP sampling protocol How to Draw Select the Scanning method Scanning a Method Selection Set the method parameters Click anywhere on the sample image to set the start point Click again to set the end of the first scan line Click again to set the number of scan lines am mM DidiL E uii m E General Operations Gas Managemert Video Operations Lighting Controls Camera Controls Triggering ETT Siac eat lt Scanning x O Draw Measure Manual Laser Control Launch Sequence Editor Start Seq
58. ator s Manual Chapter 3 Using the DigiLaz G2 Software TIPS To maximize contrast and to see the full effect of the polarizer for thin sections turn off the upper ring light and increase the lower transmitted light To see surface textures or to see craters left by previous ablations reduce the lower transmitted light and increase the upper ring light Use reflected coaxial light to see small features at high zoom levels Camera Controls The Camera Controls tab allows you to adjust the camera so that you can see the sample clearly as you set up an ablation sequence See Loading and Saving Camera Settings on page 61 and Adjusting the Camera Settings on page 62 Gas Managemert Video Operations Lighting Controls Camera Controls 8 FA SN E Adjust Settings Save Current Settings Load Saved Setting Defined Presets Figure 3 27 Camera Controls Tab Loading and Saving Camera Settings When you first start the DigiLaz G2 software the camera settings will be the same as when the software was last shut down To reset the settings choose the Default preset Controls Camera Controls ReflectedLight ThinSection TransmittedLight Figure 3 28 Default Camera Preset To use settings which are appropriate for a particular kind of sample make a selection from Defined Presets To further adjust the settings see Adjusting the Camera Settings on page 62 To save the current camera Settings as an XM
59. clear of their slots and pull out the sample drawer 3 Load the sample into the sample cell Place the sample such as a 27 mm x 46 mm petrographic slide or a NIST 612 glass standard for system optimization on the sample holder 4 Gently push the sample drawer back until it stops EEA ET Figure 2 17 Installing the Sample Drawer The sample drawer will engage the magnetic interlocks when it is fully installed If you do not push the drawer all the way in the interlocks will prevent system operation 5 Tur the pins to seat them in their slots 33 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 2 Using the Laser Ablation System Figure 2 18 Locking the Sample Drawer 6 Turn the locking knobs clockwise to seal the sample drawer Figure 2 19 Sealing the Sample Drawer Once the sample drawer is in place the system will purge the cell to remove entrained air from the cell prior starting the plasma 7 Click the Return button in the software Ablating the Sample 1 Turn on the sample cell illumination lamp Video Operatiors Lighting Controls Ca Upper Light Controls 2 Zoomall the Way in focus then zoom out to the desired magnification 3 Set up the ablation method in the DigiLaz G2 software 34 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 2 Using the Laser Ablation System 4 Set up the analysis method in the ICP software 5 Click Start Ablation Once the analys
60. cy parameters Sequence Editor This section contains the Launch Sequence Editor button which is used to open the Sequence Editor page The Sequence Editor is described in detail on page 71 Pointer Mode This section contains four selection choices Target Draw Edit and Measure 50 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 3 Using the DigiLaz G2 Software Table 3 2 Pointer Modes Mode What it does Target This selection allows the user to navigate the sample with simple mouse clicks on the graphical interface screen When a mouse click is made the stage will move such that the crosshairs are centered on that point Draw amp Edit Allows method graphics to be drawn and edited on the graphical interface screen This process is described in detail in the Draw amp Edit Method Graphics section Measure This selection allows the user to make sample measurements on the graphical interface screen as depicted below in Figure 3 9 This is achieved by clicking the left mouse button to designate the start and end points The measurement tool will give real time measurements as the measurement line is dragged out i H DigiLaz G2 General Operatiors Gas Managemert Video Operatiors Lighting Contrals Cam m Target Edit NC ETE PLUME IRE Single Line Scar xm 2 Draw i Measure Manual Laser Contral Launch Sequence Editor Method Select Figure 3 9 Measurement Tool Showing the D
61. d for future use The software also gives you control of laser operation gas flow and the real time camera display e D A Diglazc2 General Operatiore Gas Management Video Operations Lighting Contro Camera Controls Triggering e Main Single Line Scan A Toolbar Method Selection Manual Laser Control Launch Sequence Editor Drawn Method Parameters Soot Sze Microns 40 Laser Energy 9 Method eee Parameters Shutter Delay s Scan Rate micrans s m Ans Controls RTA Qm ox c AE ah Stage Controls Total Sequence Run Tme Energy N A Helium 1 Flow 0 mL min Fluence N A Helum 2 Flow 0 mL mn Irradiance N A Additional Flow 0 mL min X 24014 Y 738 89 Laser Interlock Closed Figure 3 1 DigiLaz G2 Software Main Screen 43 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 3 Using the DigiLaz G2 Software HEUS Installing the DigiLaz G2 Software If you are installing a complete laser ablation system the software will be pre installed on the supplied computer If you are upgrading the software or if you are using a different computer follow these instructions 1 Ifyou have not already done so prepare the computer and the video adapter according to the document Installation Instructions for the DigiLaz G2 Software Package Insert the software CD into the host computer Select the DigiLaz G2 installation folder on the CD Double click the setup program At the We
62. ding the use of the binder prevents contamination and simplifies sample preparation Powder samples are compacted into a solid pellet using a pellet press The compacted pellet may then be analyzed just as other type of solid material To avoid problems when making powder sample pellets gt Loosely compacted pellets may break apart during ablation and provide poor or invalid analytical results Select a die for the press that is adequate for the application 13 mm or 31 mm stainless dies are most commonly used Select a 10 ton pellet press or one capable of producing at least 10 000 PSI 70 000 kPa compaction pressure for the selected die size gt For best results the powder should be ground as finely as possible Grain sizes of less than 1 5 um provide the best results for pressed pellets Pressing under vacuum also achieves better results Do not compress volatile or dangerous powders Please read the MSDS sheet prior to compressing unknown powders and other solid materials NOTE In the event the sample cell window becomes cloudy over time it needs to be cleaned or replaced See page 102 42 3 Using the DigiLaz G2 Software Use the DigiLaz G2 software to control the operation of the LSX 213 G2 or LSX 266 laser ablation system DigiLaz G2 allows you to define ablation patterns called methods by drawing them on the screen The method parameters and coordinates are stored as a sequence which can be edited and save
63. e date of shipment of any CETAC unit manufactured or supplied by CETAC and found in the reasonable judgment of CETAC to be defective in material or workmanship will be repaired by CETAC without charge for parts and labor The unit including any defective part must be returned to CETAC within the warranty period The expense of returning the unit to CETAC for warranty service will be paid for by the buyer CETAC s responsibility in respect to warranty claims is limited to making the required repairs or replacements and 117 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 6 Troubleshooting the Laser Ablation System no claim of breach of warranty shall be cause for cancellation or recession of the contract of sale of any unit Products may not be returned which are contaminated by radioactive materials infectious agents or other materials constituting health hazards to CETAC employees This warranty does not cover any unit that has been subject to misuse neglect negligence or accident The warranty does not apply to any damage to the unit that is the result of improper installation or maintenance or to any unit that has been operated or maintained in any way contrary to the operating or maintenance instructions as specified in the CETAC Instruction and Operations Manual The warranty does not cover any unit that has been altered or modified so as to change its intended use Any attempt to repair or alter any CETAC unit by anyon
64. e HE M tM 127 Operating ERVIFODIBO s sosbenbiieu netta rd a id du S ders d 127 Explanation of Caution and Warning Notices ss 128 Aver ussemernts en Francais aat aesiatek t isiot stats u eee eror tii nts in aestu ae 129 Electromagnetic Interferents 130 Explanation oft Resulatory MaPKS5 iip ri repre nean ap RR ON iei Is 131 SB QGIOSSAEV souaoweesnnmatinii e v0 dai po davor FIC DER RUN Y UR RAV RURAL 133 CETAC LSX 213 G2 LSX 266 Operator s Manual Contents This page is intentionally blank 1 Introduction Overview The Teledyne CETAC Technologies Photon Machines LSX 213 G2 and LSX 266 laser ablation systems provide a means of rapid direct analysis of solid samples without dissolution and with minimal sample preparation The laser ablation system features a high energy laser and computer controlled sampling methods using the DigiLaz G2 software The laser ablation system can be installed on any ICP OES or ICP MS Oy 1 CORC rec NOLOGIE gt Photon minimes ine Figure 1 1 The LSX 213 G2 Laser Ablation System The laser ablation system generates particulate aerosols from solid material by an extremely rapid interaction between high energy UV laser pulses and the sample surface This process is referred to as ablation Adjusting laser energy spot size and pulse frequency using the DigiLaz G2 software optimizes signal intensity and stability Ablated material is swept into the ICP MS o
65. e laser contained within the enclosure is a Class 4 laser under U S regulations If the interlocks are defeated or bypassed there is a laser radiation hazard when the top cover is open or the shield is lowered The laser contained within the enclosure is a Class 4 laser under U S regulations If the interlocks are defeated or bypassed there is a laser radiation hazard when the top cover is open or the shield is lowered 125 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 7 Safety and Regulatory Information Coolant The cooling system located in the laser power supply is filled with DISTILLED WATER Do not fill with any other type of fluid The cooling system should contain a sufficient amount of distilled water The fault light will come on if the coolant is in interlock mode Ventilation The laser power supply cooling fan should remain unobstructed at all times Do not operate the instrument if the cooling fan is blocked or obstructed in any manner Mechanical Hazards If you insert any part of your body between the translation stage and the base or sides of the laser ablation system while it is operating you could be injured The maximum speed of movement is less than 1 centimeter per second so the primary hazard is pinching Figure 7 2 shows the location of potential pinch hazards Figure 7 2 Overview of Mechanical Hazards LSX 213 G2 with standard cell is shown 1 PINCH HAZARD Keep fingers hair
66. e other than by CETAC authorized personnel or agents will void this warranty In addition the warranty does not extend to the repairs made necessary by the use of parts accessories or fluids which are either incompatible with the unit or adversely affect its operation performance or durability CETAC reserves the right to change or improve the design of any unit without assuming any obligation to modify any unit previously manufactured THE FOREGOING EXPRESS WARRANTY IS IN LIEU OF ALL OTHER WARRANTIES EXPRESSED OR IMPLIED INCLUDING WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE CETAC S OBLIGATION UNDER THIS WARRANTY IS STRICTLY AND EXCLUSIVELY LIMITED TO THE REPAIR OR REPLACEMENT OF DE FECTIVE PARTS AND CETAC DOES NOT ASSUME OR AUTHORIZE ANYONE TO ASSUME FOR THEM ANY OTHER OBLIGATION CETAC ASSUMES NO RESPONSIBILITY FOR INCIDENTAL CONSEQUENTIAL OR OTHER DAMAGES EVEN IF ADVISED OF SUCH POSSIBILITY INCLUDING BUT NOT LIMITED TO LOSS OR DAMAGE OF PROPERTY LOSS OF REVENUE LOSS OF USE OF THE UNIT LOSS OF TIME OR INCONVENIENCE This warranty and all matters arising pursuant of it shall be governed by the laws of the State of Nebraska United States Returned Product Procedures Claims for shipment damage evident or concealed must be filed with the carrier by the buyer CETAC must be notified within ninety 90 days of shipment of incorrect materials No product may be returned whether in warranty or out o
67. e user will be required to correct the interference at his expense MODIFICATIONS The FCC requires the user to be notified that any changes or modifications made to this device that are not expressly approved by Teledyne CETAC Technologies may void the user s authority to operate the equipment CABLES 130 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 7 Safety and Regulatory Information Connections to this device must be made with shielded cables with metallic RFI EMI connector hoods to maintain compliance with FCC Rules and Regulations CANADIAN NOTICE This digital apparatus does not exceed the Class A limits for radio noise emissions from digital apparatus as set out in the interference causing equipment standard entitled Digital Apparatus ICES 003 ofthe Department of Communications AVIS CANADIEN Cet appareil numerique respecte les limites de bruits radioelectriques applicables aux appareils numeriques de Classe A prescrites dans la norme sur le materiel brouilleur Appareils Numeriques NMB 003 edictee par le ministre des Communications Explanation of Regulatory Marks Do not dispose in domestic household waste The affixed label indicates that you must not discard this electrical electronic product in domestic household waste in compliance with the European Waste Electrical and Electronic Equipment Directive WEEE 2002 96 EC For instructions on how to return end of life equipment producer s
68. ell or active 2 volume HelEx cell for the LSX 213 G2 Laser cooler power supply with remote control pendant and Keys with its own manual Desktop brick power transformer PC with monitor keyboard and mouse with its own manual Motion control adapter card installed in the PC LSX 266 only Cart Cables tubing hoses and fittings Test sample NOTE Please contact Teledyne CETAC Technologies 800 369 2822 1 402 733 2829 or your local sales and service representative if you need additional accessories not listed need added features to integrate the laser ablation system into your analytical system or have unique requirements Research and development of new features and accessories for the laser ablation system are inspired by customer requests and responding to such requests is a continuing activity of Teledyne CETAC Technologies SSS SSS qu cp t ti System Characteristics LSX 213 G2 Laser VV VV ON V WV Frequency quintupled Q switched Nd YAG laser 213 nm Spot size range 4 200 um with standard aperture custom apertures are available gt 3 mJ pulse laser energy computer controlled Laser output energy is adjustable from 0 100 Flat top laser beam energy profile Laser pulse width lt 5 nsec typical Laser repetition rate 1 20 Hz Optical variable attenuation Step resolution 0 16 um X Y axes 0 78 um Z axis LSX 266 Laser gt gt Frequency quadrupled
69. en Spots Microns gt Laser Energy Laser Shot Frequency Hz 2 Shutter Delay s 00 E Burst Count uo E miis 12 tf Qm xy gt Focus edu vb r DigiLaz G2 allows a number of different methods to be combined into a sequence As shown above all of the different methods can be drawn on the sample image simultaneously and ablated in order Parameters can be changed for the drawn methods at any time using the sequence editor 93 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 4 Software Methods Sequence Editor x PAS Sequence Editor General Editing Sequence Help Bgx y New Sequence Open Sequence Save Sequence Expart To Excel Import From Excel Print sequence Print Setup Main Sequence DepthProfile 1515 53 50 69 Rastering Calibration Line 62 8 67 6 423 61 l Single Line Scan Line 1 az 35 552 36 1541 94 i Raster Line Line 2 1 43 89 50 69 1770 69 D Raster Line All drawn method graphic coordinates and associated parameters are automatically stored in the sequence editor All method parameters can be changed at any time The Run Sequence button can be used to run all methods or individual rows can be highlighted to run select methods from the sequence 94 5 Maintaining the Laser Ablation System Routine maintenance of the laser ablation system consists of verifying safety systems performance and maintaining the cooling system
70. equence ECTS Video Mode Method Selection Drawn Method Parameters Spot Size Microns 40 v Space Between Spots Microns Laser Energy 96 100 s Laser Shot Frequency Hz 20 v Shutter Delay s 10 5 Burst Count 100 21 Axis Controls A tt Soe D s Ld 85 U A U N CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 4 Software Methods a High Resolution Raster Description High resolution raster expands an area raster into individual spots The Raster method treats the entire area as a single sample High Resolution Raster treats each spot as a separate sample This is useful for profiling the composition of a surface Each spot in the high resolution raster can be adjusted moved or deleted This is useful for scanning an area without sampling a particular spot and also to allow a discrete pause between each sampling point to separate the analytical results for each point Parameters Before the raster is expanded the parameters are the same as for area raster After the raster is expanded the parameters are the same as for spot scan How to Draw Select the Raster method Raster Method Selection Set the method parameters Click anywhere on the sample image to set the start point Click again to set the end point Launch the Sequence Editor 86 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 4 Software Methods DigiL Camera Controls
71. eservoir the pump will shut off and the backlights in the reservoir will blink Continue to add coolant until the coolant lines are full and the coolant level visible in the reservoir is above the depression Disconnect the fill bottle and vent tube and empty all remaining coolant from the fill bottle When the coolant is above the minimum level the reservoir backlights will remain on continuously Draining the Cooler Power Supply l To remove the coolant from the reservoir connect the vent tube to the vent fitting and then connect the empty bottle to the fill drain fitting Loosen the cap on the bottle and lower the bottle below the ICE450 as far as it can go Lowering the bottle will cause the coolant to drain from the system This is adequate to remove most of the coolant from the system But because water is used as the coolant this is not sufficient to prepare the ICE450 for shipment when freezing conditions are present See the next step for instructions on how to ship the unit in freezing conditions 2 Ifthe system is being prepared for shipment it is necessary to remove ALL water from the system because there are places within the ICE450 where water becomes trapped and irreversible damage occurs to the internal components when the water freezes Disconnect the coolant lines from the ICE450 and drain the water from the reservoir follow the steps described in Step 4 Then disconnect the coolant lines from the laser head and reconnect
72. f the laser ablation system cabinet pOWER O pC POWER IN P ams Lal Figure 2 20 Power Switch in ON Position 36 Aa W N HB CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 2 Using the Laser Ablation System E Storing the Laser Ablation System Keeping the laser cooler power supply on for long periods of time does not hurt the system In fact by continuously pumping water through the laser cavity the cooling water is much less likely to become contaminated In all cases once restarted allow the cooler power supply to run for about an hour to thermally stabilize Storing for Less Than One Week If the system is used more than 2 times per week it is advisable to keep the system and cooler power supply running Storing for a Few Weeks If the system will be stored for longer than one week turn off the cooler power supply by turning the key to the off position The cooler power supply and the laser system should not be allowed to sit for extended periods of time without running To keep the coolant clean and algae free allow the cooler power supply to run for at least a 24 hour period once per week The laser cabinet does not need to be switched on NOTE Run the cooler power supply for at least 24 hours every week Storing for Over Two Months If the unit is going to be stored for an extended period over 2 months Shut down the laser ablation system page 36 Turn the key on the coole
73. f warranty without first obtaining approval from CETAC No replacements will be provided nor repairs made for products returned without such approval Any returned product must be accompanied by a return authorization number The expense of returning the unit to CETAC for service will be paid by the buyer The status of any product returned later than thirty 30 days after issuance of a return authorization number will be subject to review Shipment of repaired products will generally be made forty eight 48 hours after the receipt Do not return products which are contaminated by radioactive materials infectious agents or other materials constituting health hazards to CETAC employees 118 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 6 Troubleshooting the Laser Ablation System Returned Product Warranty Determination After CETAC s examination warranty or out of warranty status will be determined If a warranted defect exists the product will be repaired at no charge and shipped prepaid back to the buyer If the buyer desires an air freight return the product will be shipped collect Warranty repairs do not extend the original warranty period If an out of warranty defect exists the buyer shall be notified of the repair cost At such time the buyer must issue a valid purchase order to cover the cost of repair and freight or authorize the products to be shipped back as is at the buyer s expense Failure to obtain a
74. face or a designated rectangular region of the sample This makes it easy to navigate to locations on the sample which are outside the immediate field of view of the camera You can also use the resulting set of images as a record of the entire sample before ablation Creating a Sample Map Find a zoom level focus and camera settings which show the sample clearly Don t change these settings until you are done creating the sample map Higher zoom levels will result in more images and thus a larger higher quality image but will take longer to construct 64 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 3 Using the DigiLaz G2 Software Calculate the width and height of the sample map in terms of the number of viewport sized tiles You can measure the width of the video image using the Measure tool on the General Operations tab You can measure the width of the sample by moving the cursor to the left and right edges of the sample and recording the X coordinate from the bottom of the screen Example calculation Width of the sample image 5000 microns Left edge of sample 7000 Right edge of sample 8000 Width of sample map 8000 7000 5000 3 images wide If the sample is not centered in the cell move the crosshairs to the center of the sample Set the camera controls to optimize the appearance of the entire sample map A good place to start is to click the One Push button on the Color tab On the
75. folder to the Documents library in Windows Open Windows Explorer and click on the Documents Library The heading will include a link that shows how many locations are present usually 2 locations Click on this link and follow the on screen instructions to add the Sequences folder to the library Print Operations This group allows you to print a sequence Click Print Setup to configure fonts and page layout then click Print Sequence to print to the default printer When you find a set of print settings that you like you can save the settings General Headers Footers Marging Preferences Save settings A Load settings ll Save ae Editing This tab allows features for easily editing various aspects of the sequence 72 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 3 Using the DigiLaz G2 Software a B P B eo Sequence Editor ES General Editing N Sequence Help AY 3 js Es X Cut From Grid Copy From Grid Paste To Grid Fill Down Delete Row s Figure 3 38 DigiLaz G2 Sequence Editor Editing Tab These features can either be accessed in the Editing tab by clicking each button individually or by right clicking the mouse Sequence The Sequence tab includes four groups Sequence Operation Sample Selection Laser Activity and Total Sequence Run Time e 5H Sequence Editor oe General Editing Seq aoa Help 7 Run Highlighted Method s 2 Shut down laser between samples i Run Al Methods gt
76. gged into an outlet which has a protective ground connection Power Cord to Grounded AC Power Outlet To DC POWER IN on Cabinet Figure 2 6 Desktop Brick Power Supply Ensure that you position the laser cabinet so that the location where the power supply cord plugs into it is easily accessible is not blocked and it can be quickly disconnected if needed In case of hazard the system should be disconnected from the power source The power supply socket is on the back of the laser cabinet below the power switch Connect the power supply to the laser cabinet first then connect a line cord to the power supply Do not apply power to the power supply until ready to operate the system If the power cords are not of the correct type for your country contact Teledyne CETAC Technologies See Power Cord Requirements and Safety Maintenance on page 126 Connect the power supply to the DC POWER IN connector as indicated 25 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 2 Using the Laser Ablation System DC POWER IN Figure 2 7 Connecting the Cabinet to the Power Supply ICP Instrument Connection Consult with CETAC for instructions on how to connect the REMOTE connector on laser ablation system to your analytical instrument This connection provides triggering signals Typically a custom cable will be supplied Connect the cable to the REMOTE connector REMOTE Connector Figure 2 8 Connection to Instrument
77. h sync on the power supply to Q switch on the laser If the system is being self installed and you are not familiar with the connections you may contact your CETAC representative for additional installation advice These connections are identical for the LSX 213 G2 and LSX 266 Attenuator Laser Power Coolant Q Switch and Laser Safe Figure 2 4 Connections to Cooler Power Supply Laser Power Electronics Coolant Out Power and Serial Connection Coolant In Figure 2 5 Cooler Power Supply 24 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 2 Using the Laser Ablation System Power Connection The laser cabinet but not the laser is powered by the supplied external desktop brick power supply Place the laser cabinet within 1 2 meters of a power outlet which is capable of supplying 20 amps at 120 volts or 10 amps at 220 240 volts SHOCK AND FIRE HAZARD Use only the provided power supply The power supply must be plugged into an outlet which has a protective ground connection The cooler power supply the desktop power supply the PC and the monitor must be plugged into grounded outlets A surge protected power strip with an appropriate current rating may be used The laser cabinet is intended to operate from DC power supplied through the provided brick power supply This power supply accepts a 100 240VAC mains supply A grounded power cord must be used and the power cord must be plu
78. he system has been installed by a qualified Teledyne CETAC Technologies representative the electronic connections from the host computer to the laser cabinet should already be in place For the LSX 213 G2 there are two USB connections between the laser cabinet at the host computer one to carry control signals and one to carry video from the camera to the host computer For the LSX 266 there is one USB connection to carry video a 68 pin control cable and an RS 232 serial cable Video Out USB to Computer Control USB to Computer Pe Iz VVVVT VY YVTS r AADO Ay Ay DOO Na Hte ae eee ADDN AAA EA T PAA X fat OOO AD YS nS 14 T 2229200000000 008 I i EE f f H TM i b i RS232 Serial Data 68 Pin Control Cable m Y USB Video USB Mouse and Keyboard USB Control RS232 Serial Data uw E 68 Pin Control Cable A W heiss aisse Figure 2 3 Host Computer 23 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 2 Using the Laser Ablation System Connections to the Cooler Power Supply Likewise all connections between the laser and the power supply should be in place including the water lines the primary power cable connecting the power supply and laser the RS232 cable from the power supply to the laser the BNC coaxial connecting the remote link connector on the power supply to the Laser Safe connector on the laser and another BNC cable connecting the Q switc
79. iling method allows the user to set a rate at which the sample stage moves to keep the laser beam focused at the bottom of the crater If one uses depth profiling to determine changes in elemental composition with depth analyzing a coating for instance various steps with different parameters can be set to compensate for changes in the ablation characteristics of the layer When a standard of known coating thickness is used one can determine how many laser shots correspond to a given depth and from there use elemental signals to determine the depths of coatings Up to 10 steps can be used with variable Z rate and time for each step For most applications 1 step is sufficient since the Z rate should be constant however multiple steps are provided to give maximum flexibility for this method A pause between each step can also be set to allow separate layers to be separated in the ICP ICP MS data Parameters The drawn method parameters are similar to the Single Point method spot size laser energy shot frequency and Q switch delay The burst count is calculated based on the laser shot frequency and time for each step Additional parameters in the sequence editor are Z travel time and post step delay The Z rate is automatically calculated by dividing the Z travel by the time These are accessed in the Depth Profile tab of the Sequence Editor How to Draw 1 Select the Depth Profile method Depth Profile Method Selection 2 Set
80. is type and area are defined ablation can begin The laser ablation system will operate under computer control until it reaches the end of the sampling procedure or it is interrupted by pressing the Stop icon See Software Methods beginning on page 77 for more information about setting the laser s operating parameters and drawing method graphics 35 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 2 Using the Laser Ablation System T Shutting Down the Laser Ablation System Emergency Stop To stop the laser quickly gt Press the stop button on the power supply pendant control pod or gt Turn off the power switch on the back of the laser ablation system In Case of Electrical Hazard If there is an electrical hazard for example if a liquid has spilled into the power supply unplug the laser power supply and the laser ablation system from the wall outlet Normal Shutdown To shut down the laser ablation system complete the following steps Ifa laser method is running or if the laser is firing click on the appropriate stop command When a method is completed the laser is automatically turned off If using the manual controls turn the laser off with the STOP button Exit from the DigiLaz G2 software and shut down the computer if desired Choose then Exit to shut down the DigiLaz G2 software Turn off the cooler power supply Turn off the laser cabinet The switch is located on the back o
81. istance between Two Points shown as signs 51 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 3 Using the DigiLaz G2 Software Method Selection Use the button in this section to choose which method to draw on the screen Figure 3 10 A Single Line Scan NO Single Line Scan de Rast LEE i E Er Raster Line Scanning Single Point atal MA liuc T4 Depth Profile lil Multi Line Scan Figure 3 10 Method Selection Menu Sequence Operations Use these commands to start and stop a method or sequence Click Start Ablation to open the Sequence Run Information window This window Figure 3 11 displays the status of all relevant information during a method or sequence and also allows real time control of energy and laser shot frequency DigiLaz G2 Sequence Running Processing Sample 1 of 1 Progress on Current Sample Progress on Batch QJ Laser On Laser Energy Shutter Open Laser Shot Frequency Hz Trigger Inactive Gas Blank Delay 0 sec Inter Sample Delay Figure 3 11 Sequence Run Information Window 52 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 3 Using the DigiLaz G2 Software Gas Management The Gas Management tab consists of four sections Helium Flow Control Gas Management State Purge Time and Cancel Purge a E p E DigiLaz G2 General Operations Gas Managemert Video Operations Lighting Controls Camera Controls Triggering Helium i
82. iterations of method development are required to produce precise and accurate results The laser ablation system should be optimized in concert with the host ICP system to make certain that the best laser method has been developed Elemental or Spatial Mapping Laser ablation sampling can be used effectively to the spatial distribution of elements on the surface of a sample The DigiLaz G2 software has been developed to make this task very simple Most frequently the multi line scan ablation method is used for introducing sample aerosols as this provides data that is most easily processed into images Data is collected as element signal intensity vs time so spatial or elemental maps can be created to show trace and major elemental variations as a function of matrix The raster method may also be used to introduce sample aerosol that has been generated by a specific number of laser shots at specific points however data processing may not be as straightforward 39 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 2 Using the Laser Ablation System Select the appropriate laser ablation method Optimize the ICP or ICP MS system to produce desired mapping elements in the format of time resolved mode Adjust laser parameters scan rate pulse rate spot size and energy to suit the application and desired data quality Start the ablation Depth Profiling Using the laser ablation system for measuring matrix analyte concent
83. k Last Segment Use the Sequence Editor to adjust the parameters Canceling a Method Graphic In Draw mode a graphic in process of being drawn can be canceled before the end point is set by right clicking and choosing Cancel Sample from the menu To delete a method graphic immediately after it was drawn Right click anywhere on the sample image Select Delete Last Sample or Delete All Samples y m Cancel Sample Mark Last Segment Edit Point Delete Sample s D elete Last Sample Delete All samples Deleting a Method Graphic Visually To delete a previously drawn method graphic or to delete one point or line segment from a complex method Click the General Operations tab Click Edit in the Pointer Mode group Right click on the point or on the beginning of the line segment to delete Deleting a Method Graphic in the Sequence Editor To delete a point or line segment in the Sequence Editor Click on the row number to select the entire row Right click on the row and select Delete Row Editing a Method Graphic To move a point or the beginning end point of a line segment Click the General Operations tab 69 A Uu N HM CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 3 Using the DigiLaz G2 Software Click Edit in the Pointer Mode group Points which can be edited will be displayed within squares Target iq Edit Draw 2 Measure Video Mode Right click inside the square
84. k contamination gt Poor stability and poor long term precision gt Visible coating of ablated material in the transfer line When changing the tubing care must be taken when disconnecting it from the nuts on the internal valves There are two ferrules that provide the sealing pressure Loss of the ferrules may cause leakage in the tubing assembly After changing the tubing connect the laser system up to the host ICP ICP MS and light the plasma Start an ablation on NIST 612 and check the oxide levels of ThO and UO If these values are significantly higher than those achieved before changing the tubing then there may be a leak in the system Additionally you can cycle the Gas Management State through Bypass Purge and Online modes to check for stability Air leaks caused by improperly fitted tubing may cause plasma instability such as flickering or in extreme cases complete extinction of the plasma 103 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 5 Maintaining the Laser Ablation System Nebulizer Gas Helium Inlet Inlet Vent Outlet wae Rear Panel Side Panel Sample Out to ICP Bypass Pinch I oO Qu lt p Pressure Relief N Valve Block ks H pA Helium 9 Argon Sample A Mass Flow Controller Carrier Gas Select Valve 8 Sample y H Outlet Single Volume Ablation Cell Figure 5 8 LSX 213 G2 tubing diagram showing gas flow in the online state 104 CETAC
85. l remain on 100 uU A Uu N HP CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 5 Maintaining the Laser Ablation System Opening the Top Cover PINCH HAZARD Fingers could be pinched between the top cover and the main cabinet Use caution when opening and closing the top cover The top cover is held in place by two latches A 5 32 inch hex driver or hex key is required to operate the latches Figure 5 5 Right Cover Latch Figure 5 6 Right Cover Latch Viewed from Below Turn off power to the laser ablation system Shut off gas supplies Verify that no objects are resting on top of the laser cabinet Locate the two latches as shown in Figure 5 5 Use a 5 32 inch hex driver or hex key to turn the latch bolts counterclockwise 1 2 turn 101 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 5 Maintaining the Laser Ablation System Lift the cover When the maintenance is complete gently lower the cover and secure the latches Note that the laser will not operate until the cover is completely closed When you turn the power back on watch the status LED on the front of the laser ablation system If it is not illuminated when the power is on the cover is not fully closed Cleaning the Sample Cell Window Dirt in the optical path can cause unclear video images Dirt is most likely to cause problems in two places the sample cell window and the prism It is rare for dirt to cause problems
86. lation System Components The following components wear out or become contaminated under normal use and must be replaced periodically gt System tubing gt Cooling water gt Laser flashlamp Water de ionizer cartridge If you fail to replace these components when they deteriorate the laser ablation system will not function properly Purchasing Supplies Because the usage rate of consumable materials and the life span of expendable components will vary you should maintain an adequate supply of spares When you need to purchase additional supplies it is important that you choose the appropriate components and materials A one year consumables kit is available from Teledyne CETAC Technologies When you purchase replacement parts or consumable supplies make sure they meet the following requirements gt Use only distilled water as the coolant Bottled distilled 1 3 MQ water works best Do not use 18MQ laboratory water Tap water or any other coolant will leave deposits in the cooling system may damage the cooling unit or laser head or cause other malfunctions gt Donotattempt to use a substitute laser flashlamp otherwise leakage and laser head damage will result Service only with an exact replacement Use of unsuitable coolants consumable supplies or inferior replacement parts may result in laser ablation system malfunctions ICP malfunctions invalid analysis results or hazardous conditions Be sure all replace
87. lcome screen click Next Click Next to install DigiLaz G2 in the default location 2 3 4 5 6 Read the license agreement click to accept and then click Next 7 8 Review the installation settings and then click Install 9 Wait for installation to finish It may take several minutes 10 Click Finish to end the installation program The installation program will offer you the option to launch DigiLaz G2 TIP By default DigiLaz Sequences are saved in the folder C ProgramData CETAC Technologies DigiLaz G2 Sequences and Viewport Snap Shots and Videos are saved under C ProgramData CETAC Technologies DigiLaz G2 Media For easy access you can add the Sequences folder to the Documents library in Windows and the Media folder to the Pictures Videos Library Open Windows Explorer and click on the desired Library or create a new one The heading for this library will include a link that shows how many locations are present usually 2 locations Click on this link and follow the on screen instructions to add the necessary folder s to the library Running the DigiLaz G2 Software for the First Time The first time you run the software you will need to configure some options 1l Launch the Com Port Utility 44 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 3 Using the DigiLaz G2 Software 2 Click Discover Com Ports and wait until all of the ports are found Laser Com Port Laser Ap
88. le is visually in focus at 100 zoom where there is the least depth of field to introduce error the laser beam will have the optimal surface interaction After focusing the zoom can then be set for the preferred field of view Zoom Click anywhere in the control to zoom to the closest 10 increment Click or to make sequential 10 zoom adjustments Defocus When enabled defocus moves the z axis such that the focal plane of the laser is above or below the surface of the sample by a specified distance Defocus affects the diameter and profile of the ablated crater it also affects laser energy density at the surface of the sample As the sample is defocussed craters get larger but the energy density drops This can have an effect on the magnitude and stability of the resulting signal at the ICP MS instrument Polarizer Click the arrows to rotate the polarizing filter which is between the camera and the sample Manually slide the polarizing filter below the sample stage to polarize the lower light 48 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 3 Using the DigiLaz G2 Software Figure 3 6 Polarizer In Place Over Lower Light single volume sample cell configuration is shown Tabs in the DigiLaz G2 Operating Software General Operations The General Operations tab consists of several sections Manual Laser Control Sequence Editor Pointer Mode Method Selection and sequence operations
89. lected is different correct the configuration and save the changes Safety Interlock Problems Several safety interlocks in the LSX 213 G2 or LSX 266 laser ablation system protect personnel from accidental laser beam and high voltage exposure and protect the laser from overheating Cover Position Interlock Sample Cell Release Interlock Shield Position Interlock Standard Cell Only Figure 6 1 Location of Laser Cabinet Interlocks LSX 213 G2 with standard cell is shown Do not deactivate any safety interlocks Tampering with the interlocks may result in serious injury Call Teledyne CETAC Technologies Customer Service and Support if safety interlock related problems occur Laser cabinet interlocks The laser cabinet interlocks protect personnel from accidental UV laser beam exposure Two independent interlock circuits ensure that even if a switch fails unsafe laser operation will still be prevented Top cover Two laser cabinet top cover interlocks are located inside the upper compartment of the laser cabinet The microswitches open whenever the top cover is lifted ensuring that the laser cannot be operated with the laser cabinet top cover opened 111 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 6 Troubleshooting the Laser Ablation System Figure 6 2 Top Cover Right Side Interlock Sample cell release Two microswitches behind the sample cell assembly open when the sample cell is moved f
90. low laser efficiency To troubleshoot this problem complete the following steps Inspect the coolant for contamination The coolant should be clear and free from contaminants there should not be any visible particulates or organic contaminants in the coolant Black particulates are a sign of pump wear and green or black slime is an indication of organic substances algae growing in the cooling system The filter should be replaced if any contamination is evident in the system NOTE If contaminated coolant is suspected the cooling system must be completely purged and properly cleaned prior to operating the laser Contact Teledyne CETAC Technologies U S 1 800 369 2822 Outside U S 1 402 733 2829 for instructions on how to clean your laser cooling system if you find organically contaminated coolant Check the laser flashlamp age The number of times that the flashlamp has been triggered can be displayed on the power supply pendant If this number is in excess of 30 million it is likely that the flashlamp intensity and corresponding laser output energy will be low Replace the Flashlamp For optimal performance the flashlamp should be replaced approximately every 30 million shots Replace the laser flashlamp if over 30 million shots Contact Teledyne CETAC Technologies for service on replacing the flashlamp Contact Teledyne CETAC Technologies U S 1 800 369 2822 Outside U S 1 402 733 2829 for any repair ac
91. lt in personal injury l Verify that removing the remote interlock connector disables the laser power supply The remote interlock connector is located within the laser power supply and is connected to the laser cabinet via the Laser Safe port Figure 5 1 Remove the connector the status indicator light on the front of the laser cabinet should remain illuminated and the Interlock light on the Remote Pendant should be flashing Replace any malfunctioning components 2 Verify that the laser will operate only with the laser power keyswitch in the ON position Verify that the key can be removed only when the keyswitch is in the OFF position Return the laser control unit for factory service if it malfunctions Q Switch and Laser Safe from Power Supply Figure 5 1 Rear View of the Laser Ablation System Cabinet 3 Inspect the shield for any damage 4 Test the shield interlocks 96 5 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 5 Maintaining the Laser Ablation System Move the sample shield up and down standard cell or move the sample drawer in and out Frame Cell and HelEx cell The status indicator light on the front of the laser cabinet should not be illuminated whenever the sample is not fully shielded Test the linear slider interlocks For the LSX 266 and LSX 213 G2 units fitted with single volume cells an additional interlock is present on the linear slider used when loading samples Move the
92. ltage Current and Frequency Transformer 100 240 V AC 10 3 2A 47 63 Hz Installation Category CAT II Line voltage in appliance and to wall outlet Output 24 V DC 5 41 A max Laser Ablation Input System Cabinet Connector is labeled DC POWER IN DC Voltage and Current 24V 5 41A Installation Category CAT I Mains isolated Use only with the provided desktop brick transformer Laser is powered independently Laser Cooler Power Input Supply AC Voltage Current and Frequency 100 240 V AC 10 5 A 50 60 Hz Installation Category CAT II Line voltage in appliance and to wall outlet Output Connect only to the laser cabinet Subject to change without notice See the documentation which accompanies the power supply for power requirements Table 7 2 Power Requirements The entire system must be connected to a circuit which is capable of supplying 20 amps at 120 V AC Input and Output Connectors RS 232 Data Connections 68 PIN SCSI Connect only to the supplied PC and cooler power supply USB unit as described in this manual USB VIDEO OUT Q SWITCH LASER SAFE 122 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 7 Safety and Regulatory Information REMOTE 15 pin Data Connection Consult with CETAC for specific connections for your application Do not connect except as directed by CETAC Trigger input is typically contact closure between two signal lines as specified by CETAC
93. mage Humber of Image Jeigin Placement t Figure 3 35 Completed Sample Map Click Load Image The sample map will now appear below the viewport in the software and can be used for macro navigation To use it click anywhere on the sample map to center the camera on that point Gas Managemert Video Operations Lighting Controls Camera Controls Triggering Adjust Settings Save Current Settings Load Saved Setting Defined Presets e T z deoa D e Vat Drawn Method Parameters f f ex hi yt Spot Size Microns i j P Laser Energy P dl Laser Shot Frequency Hz f L I 6 2 Shutter Delay s 4 Pu J r s kd Scan Rate microns s Axis Controls RTA ae Zoom jenna aago 3 Defocus 1 25 X 36096 03 Y 33280 95 2 4235 16 Total Sequence Run Time 0 Seconds Energy N A da af 2 amp e Helium 1 Flow 0 mL min Fluence N A KN s i Other Axis Controls Helium 2 Flow 0 mL min Irradiance N A zem Toi Qe Home Center Additional Flow 4 mL min Et A IM X 37594 36 Y 32497 34 Laser Interlock Closed Purging Complete Figure 3 36 Main Window with Sample Map 66 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 3 Using the DigiLaz G2 Software 10 On the File Operations tab of the Sample Map Creator save the sample map images to the location of your choice The Sample Map Creator window can now be closed Loading and Using a Sample
94. ments meet the specified requirements To order additional supplies contact your local Teledyne CETAC Technologies representative 21 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 2 Using the Laser Ablation System ESS SS SS eae Connecting the Laser Ablation System Two people are required to lift the system cabinet Lifting should be done with a person situated on either side of the instrument Arrange the cables and tubing so that they do not obstruct walkways or create a trip hazard Overview of Data Connections The following figure shows the data cables which need to be connected Each of the connections is shown in greater detail on the following pages Laser Ablation System Video Out USB USB LSX 213 G2 only Computer Serial 9 Pin LSX 266 only COM1 Serial Step Interface SCSI 68 Pin LSX 266 only shel aaa ICP Remote Trigger Optional Trigger Interface Laser Cooler Power Supply 38 Pin D Sub Back Panel Laser Power Laser Signal Attenuator Laser Power Supply Serial 9 Pin 1010 Q Switch BNC Coaxial Q Switch Out Laser Safe BNC Coaxial Interlock In Not Labeled Pendant Figure 2 1 Overview of Data Connections 22 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 2 Using the Laser Ablation System Connections to the Host Computer Assuming that t
95. mple material into a suitable laboratory exhaust system Sample Out The SAMPLE OUT tubing can be configured on either side of the cabinet Set up the system to minimize tubing length between the laser ablation system and the ICP 27 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 2 Using the Laser Ablation System ga Carrier Gas Helium In LSX 213 G2 only Argon In Optional LSX 213 G2 only Figure 2 9 Gas Connections Using the Laser Ablation System Front Panel Indicators Figure 2 10 Status Indicator Status Indicator The LED located on upper front left of the laser cabinet informs the operator of the current instrument conditions as follows Off Either power is turned off or interlocks are NOT enabled and it is NOT possible to fire the laser at this time If the interlocks are not enabled the interlock light on the pendant will flash and the pendant will display a message Interlock BNC INTLK is in on ICE front panel See User Manual 28 1 2 3 4 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 2 Using the Laser Ablation System gt Green Interlocks are all enabled the translation stage is static or being moved into position the laser system is ready to begin and it is safe to fire the laser at this time gt Red System interlocks are enabled and the laser is firing SSS SSS SSS V Starting the Laser Ablation System After the sample is
96. n the DigiLaz G2 Operating Software seen 49 Gener OD CT OUIONS ochenta a me itn uad bnt eet 49 Gas Manmdgelnelbsusaussnenn das dapdsMoradho perdu pali cud PL dE aal 53 Video OBORGLONS i din UU Da cee aan Due DAI T AMT 60 Liang CORLEOTS oio e REIR pL aetna aaa te 60 Camer ECONO S sacs satu UNE MU MUI EM N E 61 Loading and Saving Camera Settings eee 61 Adjusting the Gamera Setting Sianta toaa iaka Ee ciis Codes 62 Initial Image Adjustments coda o ubi c HER EE 62 DHOUC dcs EA Reet Per Ptr t HM Et Mt i terc 63 EXPOS UTO usa ned DURER ESD MUR VERDURE HD FOUDRE M TROC E Hd 63 6 6 t 64 Navigating With the Sample Map itte cnin rette reete te eruca 64 Creating d Sample Mup 3a dn eno ee n mA RE 64 Loading and Using a Sample Map setenta tnnt 67 Importmg a Sample Maps nsa ep O D EE 67 Drawing and Editing Method Graphics sess 68 Drawing ad Method Graphic eie b eO dps 68 Canceling a Method Graphi iisisti 69 Deleting a Method Graphic Visually ss 69 Deleting a Method Graphic in the Sequence Editor 69 Editing a Method GFODRIG idu deua Sheard ER Cibo E based UE 69 Setting Method Parameters auus eet ee nb eel reat 70 MOVIBDO O SCQUPITUO uei nica acd rd ARP da M c Glas MM E 70 Usmo the seguenta Editor asusin ires eei setenta aeri o end 71 fco PN eo een Tea ee eee
97. on Controls Click the arrows to move the sample stage in the indicated direction You can also select Target mode on the General Operations tab then click with the mouse The coordinates of the center of the image which is the laser aiming point are shown below the image For fine control you can adjust the motion step size in microns by entering a value in the box below the controls Click Center to move the stage to 0 0 0 Click Home to move the stage to the home position Click Load to prepare the sample chamber for loading changing samples This button will turn into a Return option to return the cell to the last known position 47 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 3 Using the DigiLaz G2 Software NOTE The sample stage does not automatically move when your mouse reaches the edge of the viewport You must use the stage controls to see other parts of the sample This allows precise placement of methods at the edges of the viewport Focus Controls Click the big arrows to get close to the desired focus point then use the small arrows for fine adjustments For extremely fine control you can adjust the Z axis step size in microns by entering a value in the box below the controls Focus should be set with the zoom set to 100 For optimal coupling the laser must be in focus with the sample surface The camera focus and laser focus are set at the factory to be coincident By ensuring that the samp
98. or the Dell display can be found in the supplied booklet Dell Monitors Product Information Guide and in the Dell Flat Panel Monitor User s Guide on the supplied Dell Drivers and Documentation CD CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 1 Introduction EE Preparing for Installation Refer to the LSX 213 G2 and LSX 266 Laser Ablation System Pre Installation Guide for information on Choosing a Location Space Requirements Electrical Power Requirements Computer Requirements Gas Requirements Y VV ON V Y Unpacking the Laser Ablation System You can and should arrange for a factory authorized Service Engineer to install the laser ablation system Please keep the original packing materials in case the system ever needs to be transported or returned Ventilation Allow at least 5 cm clearance on all sides of the instrument and power supply for ventilation CAUTION Do not operate the instrument if the cooling fans are blocked or obstructed in any manner 17 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 1 Introduction This page is intentionally blank 18 2 Using the Laser Ablation System The LSX 213 G2 and LSX 266 laser ablation systems are both reliable and easy to use Before using the laser ablation system however ensure that your lab environment provides operating conditions that will yield consistent data and prolong the life of the equipment Once the proper
99. orward from its operating position This prevents operation of the laser when the sample cell is extended These interlocks are not visible without removing the side cover Figure 6 3 Sample Cell Release Interlocks Shield position Single Volume cells only Two magnetic switches alongside the vertical guide rails open when the shield is moved from its operating position This prevents operation of the laser when the shield is lowered HelEx sample drawer position HelEx cell only Systems with the HelEx cell have a fixed shield and thus do not have the sample cell release or Shield position interlocks Instead two magnetic switches open when the sample drawer is moved from its operating position This prevents operation of the laser when the sample drawer is open or removed 112 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 6 Troubleshooting the Laser Ablation System Figure 6 4 Shield Position Right Side Interlock Standard Sample Cell Laser Controller Interlocks The laser controller interlocks protect personnel from high voltage exposure and protect the laser from overheating If the laser controller cover is opened or the remote interlock has a problem the cooling system overheats or the coolant flow is interrupted the system will shut down and prevent further laser operation until the faults are corrected Coolant flow The coolant flow interlock ensures that coolant is flowing through the laser head
100. ot be getting to the cooler power supply or to the laser cabinet If this is the case the status indicator green LED on the front of the laser cabinet is off and all of the LEDs on the front of the power supply are off To troubleshoot this problem complete the following steps DANGER HIGH VOLTAGE The laser cooler power supply contains lethal current and voltage levels Do not attempt any service beyond described troubleshooting procedures l Check the indicator light on the desktop transformer Power is supplied to the laser cabinet electronics via a desktop brick transformer The light on the transformer should be green if the transformer is functioning properly If the light on the transformer is not glowing green the transformer may not be connected to a working outlet or the transformer may be faulty If the light is on follow the cord from the transformer to the rear of the laser cabinet and check for good connections If the light is green and the connections are firm cycle the on off switch on the back of the laser the laser system clicks when power is supplied 2 Checkthatthe AC line cord on the laser cooler power supply is connected to the AC outlet and the AC power switch is turned on Connect the cord if it is not already plugged in to the AC outlet and turn the power switch on using the key switch Normally the pump of the cooler will start and the lights on the front will light when the key is turned M
101. ove any ambient air before being directed back to the host instrument The Purge Time box can be used to designate a specific purge time and the Cancel Purge button can be pressed at any time during the purge to return the valves to the Online state Single volume sample cells can also be operated in an argon only state where the nebulizer makeup gas is directed through the sample cell 53 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 3 Using the DigiLaz G2 Software Nebulizer Gas Helium Inlet Inlet Vent Outlet Rear Panel H H ont rh 4 O m rn Pressure Relief Valve Block Cell Gas Inlet n Side Panel lt Helium gt Argon Sample Sample Out to ICP Bypass Pinch 7 Valve WV Mass Flow Controller CN Carrier Gas D Select Valve C Sample y H Outlet Single Volume Ablation Cell Figure 3 13 LSX 213 G2 Online Gas Management State 54 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 3 Using the DigiLaz G2 Software Nebulizer Gas Helium Inlet Inlet Vent Outlet Rear Panel m t manj hrs Side Panel lt lt p Helium 3 Argon Sample oe Sample Out to ICP Bypass Pinch Mass Flow Controller Carrier Gas Select Valve 8 Sample 7 Hi Outlet Single Volume Ablation Cell Figure 3 14 LSX 213 G2 Bypass Gas Management State Nebulizer Gas Helium Inlet T Outle
102. owed exactly as written without change EYE DAMAGE HAZARD Because the internal laser beam may cause eye damage do not open the cabinet while the system is connected to a power source Wearing glasses and contact lenses etc increases the hazard All maintenance is to be performed by an Authorized CETAC Service Provider LASER INJURY HAZARD Use of controls adjustment or performance of procedures other than those specified in this manual may result in hazardous radiation exposure CDRH Laser Product Regulations The Center for Devices and Radiological Health CDRH of the U S Food and Drug Administration implemented regulations for laser products on August 2 1976 Compliance is mandatory for products marketed in the United States Laser Safety Labels The following warnings are affixed to the laser ablation system s cabinet Do not remove or alter these labels 124 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 7 Safety and Regulatory Information Figure 7 1 Locations of Laser Safety Labels Location Label Rear panel Cabinet base and under top cover Rear panel DANGER Laser radiation when open and interlocks defeated AVOID DIRECT EXPOSURE TO BEAM This product complies with 21 CFR Part 1040 10 and 1040 11 and EN 60825 1 Class 1 Laser product Explanation This is a Class Laser Product under U S regulations There is no laser hazard during all modes of operation Th
103. pears on Port COM26 IncrediBoard Motor Controller IncrediBoard Appears on Port COM13 Energy Meter Energy Meter Appears on Port COM27 Discover Com Ports Save Settings Nothing Detected on COM26 at 38400 Figure 3 2 Com Port Utility After Ports Have Been Found 3 Click Save Settings and then Exit 4 Launch DigiLaz G2 You will initially see a dialog to select your hardware starting on the Cell MFC tab Hardware Selection Tool PL Communications Laser System Motor Control Video Card Cell MFC Helium 1 MFC 2x2 Stage F Helium 2 MFC Standard 4x4 Cel amp 4x4 Stage Additional MFC Select Cell and Stage Type Number of Mass Flow Controllers m Save Settings 41 Cancel Settings Figure 3 3 Hardware Selection Tool 5 Select your sample cell stage size and correct number of mass flow controllers installed 6 Select the Communications tab and verify that the correct COM ports were configured by the Com Port Utility 7 Click the Laser System tab 8 Use the menu on the left to select your laser hardware 9 Usethe menu on the right to select the aperture wheel you have By default LSX G2 systems come with a 14 position aperture wheel 45 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 3 Using the DigiLaz G2 Software 10 Select the Motor Control tab and verify that CETAC Incrediboard Motor Control is selected along with the correct COM port pulled from the COM Port Utili
104. pper and lower light controls on the Lighting Controls tab until you see the sample If the image is still too dark or too bright adjust either Exposure on the Exposure tab or Gamma on the Image tab Small changes to either of these settings will have a large effect on image brightness Remember to click OK to see the effect of the changes Zoom all the way in and adjust focus then zoom out to the desired level 62 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 3 Using the DigiLaz G2 Software Image Device Properties DFx 41AU02 nn mn S Gamma Figure 3 30 Image Tab Adjusting the gamma applies a nonlinear correction to the intensity of each pixel in the image This adjustment is a fast way to adjust the clarity of the video image Exposure Device Properties DFx 41AU02 REESE m Sl Brightness it 50 Gain aoo 7 Auto Exposure iese E Auto Auto Reference 28 S Auto Max Value 1 8 Sec l Auto Figure 3 31 Exposure Tab Brightness increases the brightness of every pixel in the image by a fixed amount Gain determines the amplification of the CCD s output signal and affects the contrast of the image If Auto is enabled the gain will be set automatically and you will not have control over the brightness of the image Note that high gain tends to increase the noise graininess of the image Exposure determines how long the camera s CCD collects each image Longer
105. purchase order number approval within fifteen 15 days of notification will result in the products being returned as is atthe buyer s expense 119 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 6 Troubleshooting the Laser Ablation System This page is intentionally blank 120 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 7 Safety and Regulatory Information 7 Safety and Regulatory Information Review this product and related documentation to familiarize with safety markings and instructions before you operate the instrument Characteristics See page 13 for laser characteristics Environmental Characteristics Operating Temperature Non Operating Temperature Operating Altitude Relative Humidity Pollution Degree 10 C to 30 C 50 F to 85 F 0 C to 55 C 32 to 131 F Up to 2 000 m 6 562 ft 0 to 95 non condensing Pollution Degree 2 Normally no pollution or only dry non conductive pollution occurs The pollution has no influence Occasionally however a temporary conductivity caused by condensation may be expected Table 7 1 Environmental Characteristics For indoor use only Avoid sudden extreme temperature changes which could cause condensation on circuit boards in the product 121 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 7 Safety and Regulatory Information Power requirements Power Supply Input Desktop Brick AC Vo
106. r Help Extensive online help is available by clicking the help button Figure 3 41 Help Button 76 4 Software Methods Each method can be drawn using the graphical interface as indicated in these pictures or by entering the sample coordinates into the sequence editor On screen graphical method drawing is useful because one can see exactly where the ablation spot line or area is going to take place If the start and stop positions of the laser pattern cannot be seen in the same viewport area use the Allow Scrolling option to allow the stage to move with the pointer EE Spot Scan Description Single point spot scan allows the selection of individual spots Parameters The size of each spot number of laser shots per spot Burst Count energy shot frequency and defocus value can be set in the dialog on the mid right section of the main window before each spot is drawn Also a pause between spots gas blank and trigger delay can be set using the sequence editor How to Draw Select the Single Point method then click on the sample image to set the location of the spot Each spot appears in a user selectable color yellow in this case and can be erased using the right click menu and re set until the method is complete Single Point Method Selection 77 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 4 Software Methods Example Di giL E General Operatiors Gas
107. r s Manual Chapter 2 Using the Laser Ablation System hot D es n Figure 2 14 Loading the Sample 6 Replace the sample cell top and ensure that is secure and air tight by turning it clockwise until snug about 1 6 to 1 4 turn J Gently push the translation stage back until it stops The translation stage will engage the magnets when returned to the operating position Failure to fully return the translation stage will cause interlock faults and prevent system operation 8 Raise the shield Using both hands gently press up on the arms behind the shield It should move easily Do not push up on the red portion of the shield doing so may cause it to bind Figure 2 15 Raising the Shield 9 Check that the carrier gas tubing is routed so that it will not be pinched 32 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 2 Using the Laser Ablation System Figure 2 16 Carrier Gas Tubing Arrangement Once in place the system will purge the cell to remove entrained air from the cell prior starting the plasma Typically the purge time is set to 30 seconds however the use of non standard cells may require longer purge times 10 Press the Return button in the software cc mp aaa a ee qct acc Loading a Sample HelEx Sample Cell LSX 213 G2 only 1 Press the Load button in the software 2 Turnthe locking knobs counter clockwise to loosen them rotate the pins so they are
108. r ICP OES by a carrier gas also called sweep gas Many types of samples can be analyzed using laser ablation CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 1 Introduction sample introduction including glasses coatings refractory materials powders ceramics geological samples process materials and polymers Ablation may be performed on samples of electrically conductive or non conductive materials Laser Ablation Process Typically a solid sample is placed inside an enclosed chamber the sample cell and a laser beam is focused on the surface of the sample When the laser is fired an aerosol of solid particles is produced These particles are removed from the sample cell by the carrier gas and are swept into the ICP plasma for atomization ionization and subsequent analysis Coaxial Illuminator Energy Meter Polarizer W iuminator Figure 1 2 Schematic Diagram of the LSX 266 and LSX 213 G2 Laser Ablation System pue UU RU UU tl Laser Ablation System Components The laser ablation system is composed of the following components all of which fit on the supplied cart for easy portability Laser Cabinet The laser cabinet contains the laser itself all optics apertures lighting etc The sample cell on the translation stage is easily pulled forward for access to the sample cell The electronics in the cabinet are powered by a small desktop brick transformer
109. r power supply to the off position Disconnect all power cords Drain all of the water from the cooler power supply reservoir from the tubing and from the laser cabinet See Filling the Cooler Power Supply on page 98 for instructions 37 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 2 Using the Laser Ablation System mac c gcc OD Ri Methods of Analyses The laser ablation system performs bulk analysis feature analysis surface mapping and depth profile analysis Many other applications can be developed to encompass user specific goals Often one or more methods are used together to generate data that can only be obtained by using laser sampling For example the system can be used for analysis of features and inclusions in geological material Typical detection limits of 1 10 ng g can be expected for most elements using the laser ablation system coupled to an ICP MS Detection limits for ICP OES systems are generally higher in the range 1 10 ug g Detection limits are based on sample matrix and sensitivity of the host ICP or ICP MS Several laser ablation methods are provided in the DigiLaz G2 software see Software Methods on page 77 for method descriptions Optimizing the Laser Ablation Parameters The host ICP or ICP MS is optimized in concert with each laser ablation program The laser ablation parameters can be optimized using the following general procedures Place
110. ration as a function of depth into the sample is termed depth profiling Laser sampling coupled with ICP MS has found many applications areas in semiconductor pharmaceutical and materials research The laser ablation system can be set up to ablate large spot sizes at low repetition rates in order to facilitate analyte measurement with fine spatial resolution Depth resolution will vary widely depending on material but with experimentation one can arrive at a um depth laser shot The depth profiling method is designed to provide the operator with maximum sampling flexibility using a step wise method that can vary the spot size and z stage movement in an orderly fashion We have coined this procedure gradient depth profiling Gradient profiling allows the operator to selectively ablate contaminants from the sample surface followed by concentrated power density drilling into a feature or inclusion The following general steps can be used to set up a depth profiling application Using the depth profile method select a point or several points to ablate by clicking directly on the sample image Program the desired number of profile steps energy time for each step and Z Travel Z Rate will be calculated automatically from the Z Travel and Time values the lower limit is 0 78 um s for the LSX 213 G2 and 1 25 um s for the LSX 266 Typically profiling applications are performed on ICP MS systems however some simultaneous ICP OES systems ha
111. reparing for Install atiOn eene emer ee DEED EEUU IM 17 VenO 17 Using the Laser Ablation System eene 19 Establishing Optimal Operating Conditions sss 19 Creating the Lab Environm en i uaa ip aa epum 20 Replacing Laser Ablation System Components es 21 Parcha ng S UDDITESL ica esee eterno iR OR baden i EE 21 Connecting the Laser Ablation System eene 22 Overview of Data ConnectionS sss sssssssssssssrinsnrrnsnrrnsnrnnsnnnnennrnennnnennnnennnnennnennnnenns 22 Connections to the Host Computer serrata tnnt 23 Connections to the Cooler Power Supply s 24 Power GORHOEDIOR irirna nn M E 25 IGP Instr ment CONNECTION ac ocdsotanest iones E e OEE RRi 26 GaS COC CLOG orria inan AA OO 27 Using the Laser Ablation SS UII sesiis aa aaa 28 Front Panel Indicators erstattet rettet anota noob 28 Starting the Laser Ablation System aussesssisadicni ck rsrtitunckzvEEpHrEDkeate trm rS aE rubr ertet ERE iU 29 Loading a Sample Standard Sample Cell sss 30 Loading a Sample HelEx Sample Cell LSX 213 G2 only 33 AD Ces ODRDIG e otcetene spar tom oae o terrier ener dt naire aire rarer errr 34 Shutting Down the Laser Ablation System ses 36 CETAC LSX 213 G2 LSX 266 Operator s
112. s A malfunction of the laser can indicate a problem with the laser electronics unit laser pumping energy the cooling system or with the laser flashlamp The following sections explain how to troubleshoot these problems DANGER INVISIBLE LASER RADIATION The laser ablation system uses a Class IV Nd YAG laser The output beam is by definition a safety and fire hazard Precautions must be taken during use and maintenance to prevent accidental exposure to direct or reflected radiation from the laser beam No Laser Output 1 Check all cable connections No AC power low laser pumping energy or improperly connected control cables can result in no laser output 2 Check the coolant level The laser will not operate if there is no coolant flow or the temperature is too high 3 With the AC power off and the power cord unplugged check all the electrical connections between the laser cabinet and cooler power supply Make sure all connections are secure that the cables are installed correctly and not causing the system to malfunction DANGER HIGH VOLTAGE Both the laser head and laser electronics unit contain electrical circuits operating at lethal voltage and current levels Always unplug and wait at least one 1 minute to allow capacitors to bleed down before servicing any part of the laser system 4 Operate with the manual laser controls The manual laser controls are accessible from the main software screen by pressing
113. sample chamber in and out of the laser cabinet The Status indicator light on the front of the laser cabinet should not be illuminated whenever the linear slider is not in the operating position 6 Test the cover interlocks 1 Unlatch and slowly raise the cover The status indicator light on the front of the laser cabinet should immediately go out as you begin to raise the cover IE Cooling System Maintenance Changing the laser cooling system coolant is the primary maintenance task that will be performed Other items should also be checked periodically to maintain the cooling system properly In addition the cooling system has to be drained before transportation The cooling unit reservoir must only be filled with distilled water Do not use high purity lab grade de ionized water since it will corrode the laser cooler power supply and the laser head internals The cooler power supply should not sit for extended periods of time NOTE Keeping the laser cooler power supply on for long periods of time does not hurt the system In fact by continuously pumping water through the laser cavity the cooling water is much less likely to become contaminated See Storing the Laser Ablation System on page 37 Periodic Checks of the Cooling System Inspect the coolant level in the reservoir every week Check to see that the coolant level visible in the reservoir is above the depression When coolant is above the minimum level the re
114. ser cooler power supply fuse Rating 5A Type 3AG Slowblo Replace fuses with specified type s and rating s only If the AC power is within specifications and the laser ablation system will not operate call CETAC Customer Service and Support or an authorized service representative 109 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 6 Troubleshooting the Laser Ablation System SSS SSS E Interface Problems The host computer directs operation of the laser ablation system A malfunction of the laser ablation system can indicate a problem with the cables or with the software configuration of the computer The following sections explain how to troubleshoot these problems RS 232 Cable Problems LSX 266 Only The first step in troubleshooting suspected interface problems is to check the RS 232 cable To do so complete the following steps Check the STATUS indicator green LED on the front of the laser cabinet to ensure the power is on Check the RS 232 cable to ensure it is plugged in to the COMPUTER port of the laser cabinet If the cable is plugged in ensure that the connector is properly oriented fully seated and the thumbscrews are fully and evenly tightened Check the host computer to ensure that the RS 232 cable is connected to the COM1 port or USB port if using the USB configuration If the RS 232 USB cable is plugged in ensure that it is tightened properly Control Cable Problems The first s
115. servoir backlights are on continuously indicating that the enough distilled water is present for normal operation If the back light is flashing on and off follow the steps below to fill the reservoir to proper levels 97 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 5 Maintaining the Laser Ablation System Ven je Par ol dor yen Ful Draain Connector Figure 5 2 Fill Drain Port Locations Filling the Cooler Power Supply Before shipping the laser a Teledyne CETAC Technologies technician drains the coolant from the coolant reservoir In order to use the cooler power supply the customer must fill the coolant reservoir with about 1 5 liters of distilled water Please follow the steps below l Check that all of the coolant hoses are connected in the appropriate manner 2 Fillthebottle with coolant Attach the coolant fill drain connectors to the fill bottle The hose with the single fitting connected to it attaches to the upper vent fitting Loosen the cap on the fill bottle and add coolant until it drains from the vent fitting Figure 5 3 Fill Drain Bottle and Vent Tube 3 Turn the key switch to the ON I position 98 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 5 Maintaining the Laser Ablation System 4 The pump will turn on automatically after the system is on and coolant will begin filling the coolant lines When the coolant level falls below the depression in the front of the r
116. t 70 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 3 Using the DigiLaz G2 Software For mathematically inclined users more complex transformations such as rotation and scaling can be accomplished by exporting the sequence to Excel and applying matrix transformations Remember to adjust spot size and Spacing if needed Keep in mind that raster scan method lines are always horizontal with respect to the laser display rotating the start and end points of a raster or scan will not rotate the orientation of the lines within the pattern NOTE DigiLaz G2 does not support importing Excel files with formulae To ensure proper import select the whole sheet copy it and paste back into the same sheet using the Paste as Values function in Excel before saving the edited sequence Using the Sequence Editor The Sequence Editor opened by clicking Launch Sequence Editor in the General Operations tab of the main window General Operations Gas Management The Sequence Editor automatically stores all drawn method pattern coordinates and associated parameters such as spot size pulse repetition rate scan rate etc Sequence Editor General Editing Sequence Help a Ae y BO Mew Sequence Open Sequence Save Sequence Export To Excel Import From Excel Print Sequence Print Setup Main Sequence Depth Profile 1516 53 550 69 Rastering Calibration Line 626 94 67 6 423 61 415 14 L Single Line Scan Line 1
117. t uM a n ante w Rear Panel H y Side Panel Helium gt Argon Sample EN sm BERG Sample Out to ICP Bypass Pinch Valve AJ y Mass Flow Controller x Y in Pressure Relief C N Valve Block E G f d NM V Carrier Gas Select Valve gt Sample x H Outlet Single Volume Ablation Cell Figure 3 15 LSX 213 G2 Purge Gas Management State 55 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 3 Using the DigiLaz G2 Software Nebulizer Gas Helium Inlet Inlet Vent Outlet imm Rear Panel Side Panel Helium gt Argon Sample S mese i Sample Out to ICP Bypass Pinch ur bis ln Mass Flow Controller Y A Pressure Relief f NS Valve Block Carrier Gas lect Val Select Valve Sample ys Outlet y Single Volume Ablation Cell Figure 3 16 LSX 213 G2 Argon Only Gas Management State no He flow Nebulizer Gas Helium Inlet Inlet Vent Outlet Additional Gas Inlet N Rear Panel Side Panel Helium gt Argon Sample Mass Flow Controller 3 Optional Vent Check Valve Sample Out to ICP Bypass Pinch Valve Purge Solenoid Valve M Vacuum Pump Carrier Gas Select Valve HelEx Active 2 Volume Cell Outlet Figure 3 17 LSX 213 G2 Online Gas Management State HelEx Cell 56 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 3 Using the DigiLa
118. tem It is recommended to change the coolant when changing the filter 2 Orient the ICE450 so that the rear of the unit is visible 3 Usean adjustable wrench to remove the gray nuts from the filter Remove nuts from filter Figure 5 4 Inline Coolant Filter 4 Install the new filter The gray nuts should be snug but not so tight as to damage the fittings or the filter 5 Re fill the power supply with distilled water a Fillthe bottle with fresh coolant Attach coolant fill drain connectors the hose with two leak free connectors is used between the bottom connector of the coolant bottle and the lower connector on the ICE450 the hose with one leak free fitting and one open fitting is used between the side connector of the coolant bottle and the upper connector on the ICE450 Raise the bottle and wait until the coolant fills the reservoir b Turn the key switch ON T The pump will turn on automatically after power up and begin filling the coolant lines When the coolant level falls below the depression in the middle front of the reservoir the pump will shut off and the reservoir lights will begin blinking Continue to add coolant until the coolant lines are full and the coolant level visible in the reservoir is above the depression Disconnect the fill bottle and vent tube and if using water as a coolant empty all remaining coolant from the fill bottle When the coolant is above the minimum level the reservoir lights wil
119. tep in troubleshooting suspected interface problems is to check the large control cable To do so complete the following steps Check the STATUS indicator green LED on the front of the laser cabinet to ensure the power is on Check the cable to ensure it is plugged in to its port on the laser cabinet If the cable is plugged in ensure that the connector is properly oriented and fully seated Check the host computer to ensure that the stepper cable is connected to the stepper control board Since this is a large 68 pin cable take special care when seating the cable since it is possible to bend the pins on the cable or connectors if the cable is forced When replacing the cable inspect the plug for any bent pins Software Configuration Problems If the cables are connected properly and the laser ablation system is still not communicating with the control computer ensure that the software is configured correctly To do so complete the following steps Start the DigiLaz G2 software to ensure that the computer is functioning properly 110 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 6 Troubleshooting the Laser Ablation System 2 Check the software configuration for the correct COM port selection and communications parameters 9600 N 8 1 If the laser ablation system is connected to a port other than the one defined or the baud rate 9600 parity N number of data bits 8 or number of stop bits 1 se
120. the Manual Laser Control button With a familiar sample in the cell set the spot size to 200um the energy to 100 and the shot frequency to 20 Hz Set the firing mode to burst and set the burst count to 200 Press Start Laser These settings should produce a 200um crater in 10 seconds after the shutter delay This will test the Q switch shutter and the laser itself independent of any methods 5 Observe the results of the manual laser test If operating the manual controls test above gave anything other than the round crater expected make a note of whether the power supply made the typical clicking sound whether there was any mark on the test sample at all or ifthe crater was non circular Given this information consult your CETAC service representative who will provide further guidance 114 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 6 Troubleshooting the Laser Ablation System Modification of the furnished laser power supply is done solely at the user s risk Call Teledyne CETAC Technologies Customer Service and Support if power system related problems occur Low Laser Efficiency Laser output energy below nominal may suggest only gradual lamp and or cooling system degradation These characteristics are normal over time and with large laser shot accumulations gt 30 million Contaminated coolant a laser flashlamp that needs to be replaced or a resonator in need of adjustment can all result in
121. the drawn method parameters in the main window 91 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 4 Software Methods 3 Open the Sequence Editor and select the Depth Profiling tab to set the per step parameters Main Sequence Depth Profile 4 Click anywhere on the sample image to set the location So Gas Managemert Video Operations Lighting Controls Camera Control Triggering I Target Edit RS z Depth Profile 9 Draw Measure Manual Laser Control Launch Sequence Editor Start Sequence Stop Sequence Video Mode Method Selection E E Drawn Method Parameters Spot Size Microns FE hd e TA brad 2 My 4 Ud 7 Laser Energy 99 amp Laser Shot Frequency Hz 20 Shutter Delay 5 10 5 Rf A tf Qm xy Focus Vv NOTE The Depth Profile settings apply to all Depth Profile patterns present in the Sequence Editor 92 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 4 Software Methods Multiple Method Drawing wn y E DigiLaz C E General Operations Gas Managemert Video Operatiors Lighting Controls Camera Controls Triggering Toggle Cross Hairs Toggle Lines CrossHairColor M thodColor t dD Ll e X Snap Shot Record Stop Create Sample Map Cancel Sample Mapping Cross Hairs Lines Drawn Method Parameters Spot Size Microns 25 Space Betwe
122. the laser will wait before starting the next method Trigger Delay Delays the trigger by the specified number of seconds after the pattern is started Use Trigger Delay to trigger the host instrument to begin data acquisition after the laser has begun to fire on the sample Examples In the example below a Pause Between Samples of 60 seconds is used This means after the method in row 1 is completed the DigiLaz G2 software will count down 40 seconds and then begin the Gas Blank countdown for an additional 20 seconds to equal the total 60 second pause before ablation of the next method begins The trigger signal will be sent after the initial 40 seconds has elapsed when the 20 second Gas Blank begins 74 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 3 Using the DigiLaz G2 Software 60 10 zl j 3G 35 l Represented visually a timing map for the above example shows that the Pause Between Samples is in effect the time between one ablation ending and the next ablation starting NOTE By default the trigger remains closed for 2 seconds to allow the connected ICP ICP MS to register the trigger On the Triggering tab you have the option of turning the trigger on for the entire ablation for ICP ICP MS instruments that require a constant trigger during acquisition Sequence Start Pattern 1 Pattern 2 Trigger Shutter Delay Gas Blank Ablation Pause Between Samples 90 100 110 120 130 140 150 50 60
123. tions necessary beyond those described in this manual Attempts to adjust repair or replace optics may cause additional problems and void warranties III UE E UU Carrier Gas System The carrier gas is the medium that delivers the ablated sample to the ICP MS The tubing outlets can be configured on either side of the laser ablation 115 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 6 Troubleshooting the Laser Ablation System system A rule is to use the shortest lengths of tubing to connect to the ICP MS system Carrier Gas for the LSX 213 G2 The carrier gas for the LSX 213 G2 is typically helium and is controlled by the helium mass flow controller s via the software Argon from the host ICP is used as a make up gas which is mixed downstream of the sample cell before exiting the SAMPLE OUT port on the side of the laser cabinet and is connected to the CARRIER GAS port of the laser system If for whatever reason helium is not available or desired the helium flow can be set to 0 in the control software which triggers argon from the ICP to take over as carrier gas The helium controller is a maintenance free system Helium ultra pure grade recommended otherwise the purest grade available is connected to the red push connector in the rear of the laser system with the supplied tubing 1 8 OD To remove the tubing from the push connector push the red collar inward then pull the tubing outward Carrier Gas for
124. ty 11 Select the Video Card tab and verify Image Source HD Camera is selected 12 Click Save settings DigiLaz G2 will now launch From now on when the application loads it will automatically establish communication between the computer and the laser ablation system This includes homing the translation stage homing laser hardware and verifying system interlocks When the program has initialized the main screen will appear Figure 3 1 depicts the main screen of the DigiLaz G2 Software If you need to access the configuration menu again open the Select Hardware dialog EN Jh z Manual Laser Control i File b Launch Sequence Editor p 4 T S LL al b Create Sample Map 2 Cancel Sample Mapping be Perform Zoom Calibration a Launch Energy Calibration Tool 4 Exit Figure 3 4 Opening the Select Hardware Dialog For instructions on starting and shutting down the laser ablation system for normal use see page 29 46 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 3 Using the DigiLaz G2 Software Using the Stage Controls to View the Sample Axis Controls Motion m X Y c Focus Focus Controls Controls mus Step Size Zoom 0 zz m 750m Controls Defocus Defocus Defocus Polarizer A ep 777 Polarizer Rotation Other Axis Controls Home Center Axes Home Center Load S Load Sample Chamber Figure 3 5 Stage Controls Moti
125. ty of Laser Products Publication IEC 60825 1 International Electrotechnical Commission webstore iec ch A Guide for Control of Laser Hazards Publication 0165 American Conference of Governmental and Industrial Hygienists ACGIH www ACGIH org Occupational Safety and Health Administration OSHA U S Department of Labor 200 Constitution Avenue NW Washington DC 20210 Phone 202 523 8148 www OSHA gov Performance Standards for Laser Products United States Code of Federal Regulations 21 CFR 1040 10 d and 1040 11 Additional Information from Teledyne CETAC Technologies Y YV V WV New versions of this manual may be available under Service and Support on CETAC s Web site www cetac com LSX 213 G2 and LSX 266 Laser Ablation System Pre Installation Guide Installation Instructions for the DigiLaz G2 Software Package Service notes Teledyne CETAC Technologies Customer Service and Support Phone 1 800 369 2822 USA only 1 402 733 2829 Fax 1 402 733 1932 E mail custserv cetac com Related Equipment gt 16 Regulatory and safety information for the Quantel ICE450 laser cooler power supply is located in the ICE 5 Power Supply User s Manual which can found on the USB flash drive located on the laser key ring Regulatory and safety information for the Dell PC can be found in the supplied booklet Safety Environmental and Regulatory Information Regulatory and safety information f
126. uence Stop Sequence Video Mode Method Selection Drawn Method Parameters Spot Size Microns 40 Space Between Lines Microns Laser Energy 26 100 jA Laser Shot Frequency Hz 2 v Shutter Delay s 10 iA Axis Controls KTA tf e whom LLa sit 83 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 4 Software Methods a eee eee Raster Description Raster is analogous to raster line except it covers a defined area in a similar way that scanning does Raster will ablate discreet spots from left to right over a defined area Parameters Enter the spot size and distance between spots Energy shot frequency and number of shots per spot may also be set The number of spots total area and time of analysis are automatically calculated How to Draw Select the Raster method Raster Method Selection Set the method parameters Click anywhere on the sample image to set the start point Click again to set the end of the first scan line Click again to set the number of scan lines 84 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 4 Software Methods r2 E igi E General Operatiors Gas Managemert Video Operations Lighting Controls Camera Controls Triggering a TEEN Target Edit 3333 HH eee En R Raster i9 Draw Measure Manual Laser Control Launch Sequence Editor i Start Sequence Stop S
127. uipment The WARNING notice denotes a hazard It calls attention to a procedure practice or the like that if not correctly performed or adhered to could result in personal injury Do not proceed beyond a WARNING notice until the indicated conditions are fully understood The CAUTION notice calls attention to a procedure or practice that if not correctly performed or adhered to could result in equipment damage loss of data or inaccurate data Do not proceed beyond a CAUTION notice until the indicated conditions are fully understood and met 128 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 7 Safety and Regulatory Information Avertissements en Francais This section provides French translations of notices which may appear on the instrument or on other instruments used as part of the measurement system WARNING FOR CONTINUED PROTECTION AGAINST RISK OF FIRE REPLACE ONLY WITH FUSES OF THE SPECIFIED TYPE AND CURRENT RATING FOR CONTINUED PROTECTION AGAINST RISK OF FIRE REPLACE ONLY WITH FUSES OF TH SPECIFIED TYPE AN CURRENT RATING UT AVERTISSEMENT POUR UNE PROTECTION CONTINU CONTRE LES RISQUES D INCENDIE REMPLACER UNIQUEMENT PAR DES FUSIBLES DE MEME TYPE ET AMPERAGE A WARNING HIS INSTRUMENT CONTAINS LECTRICA
128. upplied EN electrical accessories or auxiliary items for proper disposal please contact the supplier or importer In the event a supplier cannot be reached contact Teledyne CETAC Technologies customer service department at 1 800 369 2822 C The CE mark is a registered trademark of the European Community This CE mark shows that the product complies with all the relevant European Legal Directives 131 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 7 Safety and Regulatory Information This page is intentionally blank 132 8 Glossary 213nm or 266 nm Refers to the wavelength of the laser light used for ablation in the low UV part of the spectrum Analytical Instrument The instrument typically an ICP or ICP MS to which the laser ablation system is connected Aperture Laser spot size control producing crater sizes ranging from 10 200 mm The aperture is controlled by the DigiLaz G2 software CCD Charge coupled device video sensor Defocus The distance in um that the Z stage moves down which makes a larger spot size at lower energy density Depth Profile Software controlled method for spatial profiling that can move the Z stage up at a specified rate Flat Top Energy Profile Beam profile of the UV Nd YAG laser which produces superior pit morphology He Helium Used as the sample carrier gas for the LSX 213 G2 Host Computer The computer that controls operation of the laser ablation system
129. urther preparation The standard sample cell incorporates a holder for a standard 27 mm x 46 mm petrographic slide Size reduction can be used to decrease sample size so that they will fit into the standard sample cell NOTE Teledyne CETAC Technologies and Photon Machines offer a full line of sample cells including an oversize cell a spring cell also called the paper cell for document analysis a laminar flow cell and an active 2 volume cell LSX 213 G2 only Surface Conditions For most samples surface preparation is not necessary or recommended Most sample surface preparations such as grinding cutting and polishing may introduce contamination Only those samples with extremely uneven surfaces and intended for bulk analysis should be cut and ground Pre ablation can be used effectively for samples with grossly contaminated or corroded surfaces Pre ablation is performed by using the scanning or raster mode programs just as if the sample were being analyzed but at reduced laser power typically at 10 20 This operation provides a clean surface for the analytical scan without ablating excessive amounts of material 41 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 2 Using the Laser Ablation System Powder Samples Powder samples must be prepared to prevent large quantities of dust from blowing around the sample cell Many powders will press adequately without the use of a binder material Avoi
130. ve the ability to collect data using fast integration times The ICP MS should be set to collect a number of elements using short integration times in time resolved mode Data can then be manipulated to provide informational graphs of signal versus depth and so on Spatial maps can also provide useful representations as well 40 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 2 Using the Laser Ablation System NOTE The key laser parameters for effective depth profiling are laser energy spot size and frequency One optimization goal is to thoroughly investigate the effect of changing these parameters based on matrix type In this manner thin films and coatings tend to behave better at low frequencies 4 5 Hz while thicker coatings can be profiled at higher repetition rates E Sample Preparation With the LSX 213 G2 LSX 266 system sample preparation is minimal and no wet chemistry is involved Only large samples samples with extremely poor surface conditions and powder samples need preparation Incorrectly preparing the sample for laser ablation particularly powder CAUTION i i l samples can result in poor sample ablation and analytical results Sample Size Sample sizes are limited only by the sample cell design which has been calculated to be large enough for most applications Sample sizes ranging from a fraction of an inch to 2 inches 52 mm in diameter can be placed directly into the sample cell without f
131. vidual points in the raster as needed Right click a sample to edit or delete it ee Jigi Sae General Operations Gas Managemert Video Operations Lighting Controls Camera Controls Triggering m Target Edit HH eee P ESNA Raster _ Measure Manual Laser Control Launch Sequence Editor Start Sequence Stop Sequence UP tone Video Mode Method Selection Drawn Method Parameters Spot Size Microns 40 Space Between Spots Microns Laser Ener 8 ae CAL Laser Shot Frequency Hz 20 v Shutter Delay s Burst count Axis Controls RDD D r a ots Ma ds Me Ma E Walaa ja dds 88 bad CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 4 Software Methods a ne Multi Line Scan Description Draws two or more line scans Use this instead of the Single Line Scan method when you want to quickly draw multiple parallel lines Use this instead of the Scanning method when you want the scan to always begin from one end of the line rather than in a back and forth motion or when you want to specify a specific number of scan lines rather than visually mark a rectangular area or when you want to individually adjust the length of the lines to fit a non rectangular region Parameters The parameters are the same as for the Scanning method with an additional parameter to specify the number of lines How to Draw Select the Multi Line Scan method lil
132. x transformations such as rotation and scaling can be accomplished by exporting the sequence to Excel and applying matrix transformations Remember to adjust spot size and spacing if needed Keep in mind that with the raster and scanning methods the spots or lines respectively are always horizontal with respect to the camera display rotating the start and end points of a raster or scan will not rotate the orientation of the lines within the pattern Spot sizes are determined by the size of the apertures in the laser aperture wheel SSS M8 Triggering and Timing The DigiLaz G2 software can be used to designate when an external trigger signal will be sent to initiate data acquisition by the host instrument The external trigger uses contact closure to synchronize start and delays between the laser ablation system and the host ICP All triggering and timing features are set in the Sequence Editor and include the following Pause Between Samples Q Switch Delay Shutter Delay Gas Blank and Trigger Delay By default the trigger signal will be sent when the pattern begins that is the default trigger delay is 0 seconds Gas Blank Time that occurs before the physical beam shutter opens and the laser begins to fire onto the sample Gas Blank includes the Q Switch Delay time so it must be greater than or equal to the Q Switch Delay value Pause Between Samples Includes the Gas Blank time which sets the amount of time
133. z G2 Software Nebulizer Gas Helium Inlet Inlet Vent Outlet f i Additional Gas Inlet Rear Panel Side Panel Helium gt Argon Sample i Mass Flow Controller 3 Optional Sample Out to ICP Bypass Pinch rJ Valve Purge Solenoid Carrier Gas Select Valve HelEx Active 2 Volume Cell Outlet Figure 3 18 LSX 213 G2 Bypass Gas Management State HelEx Cell Nebulizer Gas Helium Inlet Inlet Vent Outlet Additional Gas Inlet N B B ER s Rear Panel ur Uu Hi H Y A Side Panel Helium gt Argon Sample i i n Mass Flow Controller 3 IN Optional Sample Out to ICP i Purge Solenoid S I s Vacuum Pump Su o Pressure Relief Valve Block Carrier Gas Select Valve HelEx Active 2 Volume Cell Sample Outlet Figure 3 19 LSX 213 G2 Purge Gas Management State HelEx Cell 57 CETAC LSX 213 G2 LSX 266 Operator s Manual Chapter 3 Using the DigiLaz G2 Software Nebulizer Gas Helium Inlet Inlet Vent Outlet Additional Gas Inlet N Rear Panel Side Panel Helium 9 Argon Sample Mass Flow Controller 3 4 Optional i Vent Check Valve Sample Out to ICP Bypass Pinch Valve IA I4 Mass Flow Controller 2 Sf n Controller 1 p N Purge Solenoid Valve Vacuum Pump Carrier Gas Select Valve

LSX-213 G2+ and LSX-266 Laser Ablation System Operator`s Manual

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