Leica FluoCombi III™
Contents
1. S M d i XX 1 me s 3 1 1 i x M 2 x pet N 92 p T a N 2 Mw Y Y y gt 27 1 d 08 Dimensions in mm 20 Leica FluoCombi Appendix LEL se S 1 S9W Fd Ud GYW YW Dimensions in mm 21 Leica FluoCombi Appendix 4 3 Optical data Leica MZ16 F and MZ16 FA Objective Planapo 5x 0 5 10447243 Factor Ax Iris diaphragm open Working distance 19 8mm Eyepiece 10x 21B Zoom Total Field of view Numerical Resolution Visible position magnification diameter aperture Ip mm structure width mm 0 71 28 4 1 6 0 084 252 3 97 1 40x 1 6 0 11 336 2 98 1 6 64x 1 6 0 17 504 1 98 2 80x 1 6 0 2 612 1 63 2 5 100x 1 6 0 24 732 1 37 3 2 128x 1 6 0 3 888 1 13 4 160x 1 31 0 34 1032 0 97 5 200x 1 05 0 4 1200 0 83 6 3 252x 0 83 0 45 1344 0 74 8 320x 0 66 0 49 1488 0 67 10 400x 0 53 0 5 1500 0 67 11 5 460x 0 46 0 5 1500 0 67 22 Leica FluoCombi Appendix Eyepiece 16x 14B Zoom Total Field of view Numerical Resolution Visible position magnification diameter aperture Ip mm structure width mm 0 71 45 4 1 6 0 08 252 3 97 1 64x 1 6 0 11 336 2 98 1 6 102x 1 6 0 17 504 1 98 2 122. 14 2 12 Mllumination LY Y Y YY uu 14 3 Operation 3 1 Parfocallty res 15 3 2 Magnification 17 4 Appendix 41 eu iiL tata 18 42 Dimensions Y Y Y ui 19 43 Opticaldata 22 44 Technicaldata 24 Leica FluoCombi User manual 1 Gene al notes 1 1 The user manual Leica FluoCombi is an accessory for the Leica MZ16 F MZ16 FA fluorescence stereomi croscopes Together with your Leica FluoCombi you receive an interactive CD ROM with the user manual for all products from Leica Microsystems Switzerland in the current EU languages User manuals and updates are also available for you to download and print from our web site at www stereomicroscopy com The following user manuals are important for your Leica FluoCombi III M2 166 2 Leica FluoCombi It describes the specific functions of the FluoCombi 17 2 160 4 for MZ16 FA and M2 160 5 for MZ16 describe the functions of the motorized and the manual version M2 216 1 describes the lamp housing and its functions M2 105 0 Leica M Stereomicroscopes It contains all the detailed information about the use of the stereomicroscope the optical data with stereo objectives and the safety and care instructions 1 267 1 Leica motor focus system describes the motorized focusing A Read the user manuals listed
3. direction of until the stop 5 diopter setting Do not look into the eyepieces during the pro cedure gt Select the lowest zoom setting Look into the eyepieces and close one eye each Observe the test object with one eye and slowly rotate the eye lense atthe respective eyepiece clockwise in the direction of until the view of the test object is sharp Repeat the same for the other eye Checking the parfocality gt Select the highest zoom setting gt Observe the test object with both eyes and adjust the focus if necessary Check the image sharpness from the lowest to the highest zoom position The image sharpness must remain constant parfocal If this is not the case repeat the procedure 16 Leica FluoCombi Operation 3 12 Micro mode gt Swing the HR objective 16 1 to the observer gt Select the lowest zoom setting gt Sharply focus the object with fine focusing 16 2 Working distance with 5x HR objective 19mm and with 20x HR objective 13mm gt Refocus little by little and continuously up to the highest zoom position Lock the fine focusing with the clamping screw if needed to avoid unintentional adjustment changes The focused position now remains sharp parfo cal and centered parcentric in stereo and micro mode for every zoom position Fig 16 HR planapo objective 5x rotated into the beam path 1 HR planapo objective 5x 2 Fine focusin
4. 5 1 for Mitutoyo Optem objectives and screw in adapter 5 2 as well as the corresponding objective Fluorescence stereomicroscope 7 5 with third TripleBeam beam path FLUOIII filter sys tem rapid filter changer and Leica lamp hous ing for mercury high pressure lamps as well as binocular tube eyepieces stand and focusing drive 1x Plan or planapo objective 7 9 Dichroic mirror of your choice Fig 6 Assembly and setup of Leica MZ16 F MZ16 FA fluorescence stereomicroscope and the fluores cence illumination are described in the respective operating instructions see p 6 Binocular tube eyepieces stands mercury vapor lamp etc are described in the operating instruc tions M2 105 0 M2 160 5 M2 160 4 M2 216 1 o y Fig 3 Leica FluoCombi 1117 Fig 4 HR planapo objective 5x 0 5 LWD o Fig 5 Adapter 1 for Mitutoyo Optem objectives and screw in adapter 2 E RET 3 Fig 6 Dichroic mirror 8 Leica FluoCombi Assembly Fig 7 Leica MZ16 with Leica FluoCombi II TM 2 3 Assembly sequence 1 Base of stand 9 or planapo objective 1x 2 Focusing drive with column manual or 10 HR planapo objective 5x or adapter and motorized and column adapter 2a Mitutoyo Optem objective 3 Focusing stop 11 UV protection screen 4 Leica FluoCombi III 12 Filter sets upon demand in the filter 5 Optics carrier changer and
5. slide for dichroic mirror of your choice Mount for UV protection screen Cc qm CO 3 Leica FluoCombi Overviews Fig 2 Leica FluoCombi 1 1x planapo objective rotated into the beam path 6 Clamping screw for fastening the UV protection screen 7 Clamping screw for locking the UV protection screen in the desired tilt position 8 Clamping screw for fastening the optics carrier in the microscope carrier of the FluoCombi 9 Clamping screw for fastening the focusing stop Leica FluoCombi Overviews 4 Contents Page Overviews Operating and function elements 3 4 1 Generalnotes 6 11 6 1 11 Diagrams caido rr ERE Rhin 6 12 Description 6 2 Assembly 2 1 Expansion 1 22 Standard 8 2 2 1 Additional components 8 23 9 24 Column with focusing drive andbaseofstand 10 25 Focusing stop 10 26 Transport anchorage 11 27 FluoCombi III and focusing drive 11 28 Opticscarrier 12 29 Objectives 13 2 10 UV protection 5 14 2 11 Dichroic mirrors
6. stage Stands High performance transmitted light base HL RCTM Transmitted light bases bright field dark field Incident light bases Anti vibration platform 500mm focusing drive coarse fine with 1p resolution Specialty stages Gliding stage Thermocontrol System Leica MATS Accessories FireWire digital camera systems for fluorescence Analog and video cameras Image processing and analysis software IM1000 FW4000 OWin Measurement graticules Leica FluoCombi Appendix 25 Leica Microsystems the brand for outstanding products Leica Microsystems mission is to be the world s first choice provider of innovative solutions to our customers needs for vision measurement lithography and analysis of microstructures Leica the leading brand for microscopes and scientific instruments developed from five brand names all with a long tradition Wild Leitz Reichert Jung and Cambridge Instruments Yet Leica symbolizes innovation as well as tradition Leica Microsystems an international company with a strong network of customer services Australia Gladesville NSW Tel 1 800 625 286 Fax 61 2 9817 8358 Austria Vienna Tel 43 1 486 80500 Fax 43 1 486 80 50 30 Canada Richmond Hill Ontario Tel 1 905 762 2000 Fax 1 905 762 89 37 China Hong Kong Tel 8522 564 6699 Fax 48522 564 4163 Denmark Herlev Tel 45 44 5401 01 Fax 445 44 5401 11 France R
7. 8x 1 6 0 20 612 1 63 2 5 160x 1 4 0 24 732 1 37 3 2 205x 1 1 0 3 888 1 13 4 256x 0 87 0 34 1032 0 97 5 320x 0 7 0 4 1200 0 83 6 3 403x 0 56 0 45 1344 0 74 8 512x 0 44 0 49 1488 0 67 10 640x 0 35 0 5 1500 0 67 11 5 736x 0 3 0 5 1500 0 67 Leica FluoCombi Appendix 23 4 4 Technical data Leica FluoCombi III Type Accessories with quick changer for Leica fluorescence stereomicroscopes MZ16 FA MZ16 F for a stereomicroscope objective 3D view and an HR objective microscopic resolution Optics Multiple coated high performance optics lead free maximum resolution with micro objective beam splitter for binocular observation Mechanics 360 rotation and lateral movement TripleBeam fine focusing safety focus stop Parfocal parcentric Objective change between 1x plan or planapo and 5x HR objectives at constant image sharpness adjustable fine focusing for micro objective Fine focusing integrated Dichroic mirrors GFP GFP GFP Plant UV Blue Green CFP YFP Texas Red DsRed CY5 CY7 magnetically fixed Micro objectives HR planapo 5x with MZ16 F MZ16 FA Numerical aperture 0 5 Resolution 1500 Lp mm Finest visible structure width 0 3um Working distance 19mm Magnification factor 4x Magnification 460x with 10x eyepieces T36x with 16x eyepieces Adapter For Mitutoyo Optem objectives Fluorescence stereomic
8. Leica FluoCombi User manual Jfeica MICROSYSTEMS Dear User Thank you for your confidence in our products We trust you will enjoy them and we wish you great success with the high quality and high performance products from Leica Microsystems In developing our instruments we place great value on simple self explanatory operation Nevertheless please take the time to read the oper ating instructions so that you know the advantages and possibilities offered by your stereomicroscope and can use them most efficiently Should you ever have any questions please consult your local Leica representative We are gladly at your service Customer service is a big thing with us Not only before the sale but afterwards as well You will find the address of your nearest local representative as well as valuable information about Leica Microsystems products and services from Leica Microsystems on our web site at www leica microsystems com Leica Microsystems Switzerland Ltd Stereo amp Macroscope Systems www stereomicroscopy com Leica FluoCombi User manual Overviews Fig 1 Leica FluoCombi III 5x planapo HR objective rotated into the beam path Operating and function elements Fine focusing with mount thread for a 5x or 20x HR objective requires an adapter Clamping screw for locking the fine focusing Mechanism for parcentric swinging to the stereo or micro mode Slot for filter
9. above before the startup procedure In particular please follow the safety instructions To maintain the unit in its original condition and to ensure safe operation the user must follow the instructions and warnings contained in these user manuals 1 1 1 Diagrams Pages 3 and 4 contain figures showing the Leica FluoCombi with the function elements Numbers in parentheses within the descriptions refer to the number of the figure and the items in the figure Example 1 4 Figure 1 is located on page 3 and the item 4 is the insert for dichroic mirrors 1 2 Description Leica FluoCombi is an accessory for the Leica MZ16 F MZ16 FA fluorescence stereomicroscopes and allows for quickly switching between a stereo objective and an HR High Resolution micro objective In stereo mode the 1x plan and planapo objectives offer generous viewing fields large working distances and an excellent depth of field for manipulating and dissecting Micro mode allows for exactly identifying the finest fluorescent features at a microscopic reso lution of 1500 pairs of lines mm The zoom is also effective in micro mode so that the magnification measures 460x using the 5x HR objective Using 16x eyepieces results in a maximum magnification of 736x The comfortable binocular observation is ensured in stereo and micro mode MZ16 F and MZ16 FA are high performance stereo microscopes for fluorescence applications The patented separate T
10. analogous with a dichroic filter 6 Binocular or video photo tube and eyepieces in the FluoCombi 17 7 Lamp housing with high pressure lamp and 13 Photo equipment if available stray light protection 14 Gliding stage or thermal stage if available 8 Supply unit Leica FluoCombi III Assembly 9 2 4 Column with focusing drive gt base of stand First attach the column adapter between stand column and base It is used to ensure that the center of the objective lies exactly over the center of the stage gt Fasten the column adapter 8 1 with the 3 hexagon head screws on the column gt Fasten the column with the column adapter on the stand base according to the operating instructions M2 105 0 2 5 Focusing stop AN The focusing stop is used for safety purposes and must always be correctly set with respect to the micro objective gt Fasten the focusing stop 8 2 to the column with the clamping screw 8 3 I Setthe focusing stop at a height so that the micro objective can never hit the stand or table surface or the object during focusing and swinging Working distance 5x HR objective 19mm 20x HR objective 13mm N With a correctly set focusing stop the two stops prevent focusing too low either manually or motorized If the focusing stop is set too low the objective could be damaged the object could be crushed or damaged if the lowest focus position is approached too guickly using t
11. g 3 Planapo objective 1x 3 2 Magnification field of view The magnification changer is effective in stereo and micro mode Magnification factors of the micro objectives with Leica HRobjective 5x 4x HRobjective 20x 8x The table with the optical data is located on page 22 Leica FluoCombi Operation 4 Appendix 4 1 Weights FluoCombi HR planapo objective 5x HR objective 10x HR objective 20x Planapo objective 1x Plan objective 1x Leica MZ16 F optics carrier Leica MZ16 FA optics carrier Optics carrier ErgoTube 10 50 Trinocular video photo tube 10096 Wide field eyepieces for persons wearing glasses 10x 21B GFP filter set UV filter set Lamp housing 106Z for 50 W Lamp housing 106Z for 100 W Hg lamp 50 W Hg lamp 100 W Stray light protection 3 220kg 0 5kg 0 2kg 0 2kg 0 74kg 0 78kg 3 420kg 3 14kg 3 82kg 1 7kg 1 595kg 0 158kg 0 04kg 0 06kg 2 26kg 2 Akg 0 1kg 0 08kg 0 58kg Leica FluoCombi Operation 4 2 Dimensions HR planapo objective 5x 055 7 5 AX M42 Ph 3P1 PLANAPO 5 0 0 50 LWD PLANAPO 5 0 Leica FluoCombi 1117 Appendix Leica FluoCombi 1 698914 26 E Ae Pn X 5 7 T Y NI x i M Tl eedem W 4 x Dd Y L if TW deu 7 i x 1 NL MU L 1 X NN
12. gt Insert the hexagon head screw 10 3 through the bore and tighten it on the focusing drive Fig 10 Leica FluoCombi from the front 1 Mount for stereo objective 2 Leica FluoCombi III 3 Hexagon head screw for fastening the FluoCombi III on the focusing drive Leica FluoCombi Assembly 11 Fig 11 Leica FluoCombi with Leica MZ16 1 Clamping screw for fastening 4 Planor planapo objective 1x the optics carrier 5 Thread for HR objective 2 MZ16F MZ16 FA optics carrier 6 Fine focusing 3 Thread for plan or planapo objective 1x 7 Clamping screw for fixing the fine focusing 12 Leica FluoCombi III Assembly 2 8 FluoCombi gt optics carrier I The optics carrier may not be laterally rotated in the microscope carrier of the FluoCombi 1117 since the 3rd beam path of the stereomicroscope and the FluoCombi III are adjusted to each other gt Loosen the clamping screw 11 1 and remove the dust cover in the microscope carrier of the FluoCombi III gt Carefully insert the optics carrier 11 2 in the microscope carrier and align it so that the optics carrier is flush with the microscope carrier gt Tighten the clamping screw 11 1 The assembly of the remaining components is similar to the binocular tube eyepieces etc see M2 105 0 and the fluorescence illumination see M2 160 0 2 9 Objectives FluoCombi is adjusted so that the focused position rema
13. he motor focus the fingers of the operator could be pinched between objective and object Fig 8 1 Column adapter 2 Focusing stop 3 Clamping screw for fastening the focusing stop Fig 9 Leica FluoCombi 1117 from below 1 Transport safety screws 10 Leica FluoCombi Assembly 2 6 Transport anchorage gt Remove transport locking screws 9 1 on both sides of the FluoCombi III and screw them into the respective threads for storing for transport at a later time The swinging mechanism is now released Always secure the FluoCombi III during transport with these two transport safety screws Otherwise the mechanism could be damaged and maladjusted 2 7 FluoCombi gt focusing drive The Leica FluoCombi 1177 must never be unscrewed All mechanical and optical compo nents are exactly adjusted at the factory To attach the FluoCombi on the focusing drive the mount thread for the stereo objective must be turned to the observer Only in this posi tion is the thread accessible for mounting the FluoCombi gt Turn the thread for the stereo objective 10 1 to the observer by using the swinging mechanism Only in this position is the thread accessible for mounting the FluoCombi III on the focusing drive gt Fit in and hold FluoCombi 117 10 2 in the two plugs at the back of the yoke not visible in the figure on the focusing drive
14. ins sharp parfocal and centered parcentric with every zoom position if used with a 1x plan or planapo objective and a 5x or 20x HR objective in the stereo and micro mode Hold the objectives during assembly and disassem bly so that they do not fall onto the stage plate Remove specimens from the stage plate gt Turn the thread for the stereo objective 11 3 to the observer by using the swinging mechanism gt Tighten the 1x plan or planapo objective 11 4 counterclockwise in the thread mount gt Turn the thread for the micro objective 11 5 to the observer gt Hold the fine focusing 11 6 or lock it with the clamping screw 11 7 gt Tighten the 5x HR objective counterclockwise in the thread mount OR gt Tighten the adapter 13 1 for Mitutoyo Optem objectives and in the thread 6 5 using the screw in adapter 13 2 gt Tighten the corresponding objective at the adapter 13 1 Fig 12 HR planapo objective 5x 1 SS Fig 13 1 Adapter for Mitutoyo Optem objectives 2 Screw in adapter Leica FluoCombi Assembly 13 2 10 UV protection screen Never work without UV protection screen gt Fasten the holder with UV protection screen 14 1 at the mount 14 2 using the clamping screw 14 3 gt Loosen the clamping screw 14 4 gt Tilt the UV protection screen 14 1 so that the operator cannot look directly onto the light spot gt Tighten the c
15. internationally in four business segments where we rank with the market leaders Microscopy Systems Our expertise in microscopy is the basis for all our solutions for visualization measurement and analysis of microstructures in life sciences and industry With confocal laser technology and image analysis systems we provide three dimensional viewing facilities and offer new solutions for cytogenetics pathology and mate rials sciences o Specimen Preparation We provide comprehensive systems and ser vices for clinical histo and cytopathology applications biomedical research and indus trial quality assurance Our product range includes instruments systems and consum ables for tissue infiltration and embedding microtomes and cryostats as well as auto mated stainers and coverslippers Medical Equipment Innovative technologies in our surgical micro scopes offer new therapeutic approaches in microsurgery Semiconductor Equipment Our automated leading edge measurement and inspection systems and our E beam lithography systems make us the first choice supplier for semiconductor manufacturers all over the world eca MICROSYSTEMS Illustrations descriptions and technical data are not binding and may be changed without notice M2 166 2en Leica Microsystems Switzerland Ltd e CH 9435 Heerbrugg 2004 Printed in Switzerland en X1 2004 RDV
16. lamping screw 14 4 Fig 14 UV protection screen 1 UV protection screen with holder 2 Mountfor UV protection screen 3 Clamping screw for fastening the UV protection screen 4 Clamping screw for locking the UV protection screen in the desired tilt 5 Slot with dichroic mirror 2 11 Dichroic mirrors Use dichroic mirrors 2 color coated in the Fluo Combi analogous to the filter sets used in the filter changer of the stereomicroscope The mount of the dichroic mirror is magnetically fixed in the mount to ensure a correct alignment gt Insert the mount with the dichroic mirror Fig 6 in the slot for the filter slide 14 5 so that the guide pin points down and the marking can be read on the outside Dichroic mirror marking GFP GFP1 GFP Plus GFP2 GFP Plant GFP3 UV UV Blue B Green G Texas Red TXR CFP CFP YFP YFP DsRED DSR 2 12 Illumination 1 Please adjust the Hg lamp precisely according to the M2 160 0 operating instructions so that you can achieve a homogenous light spot and good fluorescence even in micro mode 14 Leica FluoCombi Assembly 3 Operation 3 1 Setting the parfocality If a 1x plan or planapo objective and a 5x or 20x HR objective is used the focused position in stereo and micro mode remains sharp parfocal and centered parcentric with every zoom posi tion For this reason itis important to adjust the parfocality precisely as described We recommend
17. performing the diopter setting in transmitted or incident light If the fluores cence illumination is activated block the light using the light stop and rotate the dummy filter carrier into the beam path Use a very fine flat object that can still be focused with precision at the highest magnifi cation Zooming focusing and adjusting interpupillary distance eyecups diopter settings working distance illumination see M2 105 0 or M2 160 4 MZ16 FA and M2 160 5 MZ16 F operating instructions The following settings must be performed by every user only once Leica FluoCombi Operation 3 1 1 Stereo mode Ensure that the focusing stop 15 4 is set so that the micro objective can never hit the stand or table surface or the object during focusing and swinging see Chapter 2 5 gt Swing the 1x plan or planapo objective 15 1 to the observer gt Set the eye lenses at both eyepieces to diopter setting 0 gt Select the lowest zoom setting gt Set the working distance 15 2 with the coarse focusing drive 60mm for 1x plan 55mm for 1x planapo 15 Planapo objective 1x rotated into the beam path 1 Planapo objective 1x 2 Working distance 3 HR planapo objective 5x 4 Focusing stop gt Observe a flat test object and focus with fine focusing drive gt Select the highest zoom setting gt Optimize the image sharpness gt Rotate the eye lenses counterclockwise in the
18. ripleBeam light path for fluorescence illumination and the patented FLUOIII filter system provide highest quality fluorescence images Every existing instrument can be fitted with the Leica FluoCombi 1117 in a few simple steps 6 Leica FluoCombi III General notes 2 Assembly 2 1 Expansion diagram y 10447160 El 10 445 301 1062 10445302 dli 11 504 066 10 445 303 11 504 069 GO 10 446 271 yo 10 446 229 10 445 822 J MZI6F 10 447 064 Filter 10447284 10447 290 FlucCombi ll SC 10447285 10447291 Em 107324 10447286 10447 292 10447287 10447 293 10 447 288 10447 294 Motor focus 10447289 10447 295 10 447 041 500mm 10 445 819 10 447 157 Plan Planapo 2 10 447 085 20x HR Optem Mitutoyo 10 447 243 5x HR e 10 447 185 500mm ou C T 7 10 445 631 10 445 363 10 445 387 10 445 367 HL 10 446 352 HL RC Leica FluoCombi Assembly 2 2 Leica FluoCombi lll standard delivery Leica FluoCombi 1117 with microscope carrier Fig 3 for the MZ16 F and MZ16 FA optics car rier mechanism for swinging into the stereo or micro mode fine focusing with mount thread for an HR objective and for a 1x plan or planapo objective UV protection screen 7 11 Focusing stop with stops 7 3 Column adapter 7 2a 2 21 Additional components HR planapo objective 5x 0 5 LWD Fig 4 FAA 19mm or Adapter
19. roscopes Designation Leica MZ16 F with 16 1 zoom Leica MZ16 FA motorized with 16 1 zoom Microscope type Stereomicroscopes with patented TripleBeam third beam path and patented FLUOIII fluorescence filter system lead free glass Rapid filter changer horizontal FLUOIII quick changer for 4 filter sets Fluorescence filters Excitation and suppression filter sets GFP GFP GFP Plant UV Blue Green CFP YFP TXR DSR Cy3 Cy5 Slot for filter slide for neutral density filters Illumination TripleBeam 3rd beam path for fluorescence light 100 W or 50 W high pressure lamp chromatically corrected collector focusable lamp mount can be centered Extensive UV protection screen UV barrier filter stray light protection for lamp housing UV protection and dummy filter carrier for empty filter positions Stereo objective 1x planapo or plan 0 125 NA 2 7um resolution 2x planapo 0 25 NA 1 3um resolution 750 Lp mm Working distance stereo 60mm 1x plan 55mm 1x planapo 15mm 2x planapo Eyepieces Ergonomic wide field eyepieces for eyeglass wearers 10x 21 16x 14 with high field number distortion free lead free glass 24 Leica FluoCombi Appendix Accessories Ergonomics Apochromatic ErgoTube with variable viewing angle 10 50 ErgoWedge 5 25 Trinocular tube Motor focusing system with 0 7p resolution Gliding
20. ueil Malmaison C dex Tel 33 1 4732 8585 Fax 33 1 4732 8586 Germany Bensheim Tel 49 6251 1360 Fax 49 6251 136 155 Italy Milan Tel 39 02 57 486 1 Fax 439 02 5740 3273 Japan Tokyo Tel 81354359609 Fax 813 543 596 15 Korea Seoul Tel 82 2 514 6543 Fax 82 2 514 6548 Netherlands Rijswijk Tel 31 70 41 32 130 Fax 431 70 41 32 109 Portugal Lisbon Tel 35 1213 814 766 Fax 35 1 213 854 668 Singapore Tel 65 6 77 97 823 Fax 65 6 77 30 628 Spain Barcelona Tel 34 93 494 9530 Fax 34 93 494 9532 Sweden Sollentuna Tel 46 8 625 45 45 Fax 46 8 625 45 10 Switzerland Glattbrugg Tel 41 44 809 3434 Fax 41 44 809 34 44 United Kingdom Milton Keynes Tel 44 1908 246 246 Fax 44 1908 609 992 USA Bannockburn Illinois Tel 1 800 2480123 Fax 1 847 405 0164 and representatives of Leica Microsystems in more than 100 countries In accordance with the ISO 9001 certificate Leica Microsystems Switzerland Ltd Business Unit Stereo amp Macroscope Systems has at its disposal a management system that meets the requirements of the international standard for quality management In addition production meets the requirements of the international standard ISO 14001 for environmental management Leica Microsystems Switzerland Ltd Telephone 41 71 726 33 33 Fax 41 71 726 33 99 www leica microsystems com www stereomicroscopy com Stereo amp Macroscope Systems CH 9435 Heerbrugg The companies of the Leica Microsystems Group operate
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