Little Dipper for FISH
Contents
1. 9 IV SYS ENT OPERATION cuisine agiata ori inni rolatena 10 A Installing Baths and Centrifuge Buckets 10 B Using the Touch SCHEEN wsascicssanckevavessesussnpegassacsateonosadeuleanisnenascgeimieneaaseeyadateaeiaiensssisisiatrgasrvieseseansadeietaren 11 G Usne ME Fause Biol na 11 D Using the Emergency Stop ata 11 E Running a Test Protocol Practicing Rack Loading 12 F Running a Pre Installed Protocol eessssseessssseresssseressreeresereceosssrecosssrecosssrerosssreressrecosssrsreressessreeessseeeeee 13 G Usinge the Temperature Controlee 15 V CUSTOM PROTOCOLS aaa E O a 16 A Adding a PrOlOCO cn ei Sia TAi ANSE AN 16 B Delene Irro PR RR e E 17 VI PERIODIC MAINTENANCE usiilii a a a aana 18 A Cleaning Before Every USe esssssseresssseressssreresssreresssecesssrerosssrerosssrereosssreressseeeesssecesssreressresssreressseeeess 18 B Cl aning Alter Every USE siririn iirasnes O rr O REANA EGES 18 C MOmniVCIGaning ano CdliPrationi naiania E E 18 Vil SYSTENFCALIBRA TION iira o T E E 19 A Temperature Controller Calibration 19 B Slide Rack Position CallDta tOM soricei T E A E NRR 20 C Centrifuge Loading Calibration 21 D TOUCH eeN Cra O ea E E E E O AA A EAE A A A S 21 VIIS PCIE IGA TIONS corrain a 22 IX ORDERING INFORMATION 3 rara neS aaa eagle 22 K TROUBLESHOOTING oieri aan ii ORI IRR TIR 23 Xi SERVICE iaia nai binari nera nc rari 24 Ai MC OUT2. Fig 27 Press Start Now Fig 28 Load sample rack Fig 29 Remove rack option 1 March 2013 14 LD for FISH manual 2 0 software 1 4 for FISH SciGene Little Dipper Processor for FISH USER MANUAL G Using the Temperature Controllers Temperature controllers are used to regulate heating of the five bath positions Each controller is calibrated at the factory to provide an accurate bath temperature when operated between 35 to 95 C The following instructions are general guidelines for setting temperature s 1 Select a bath position according to your protocol and turn ON the switch above the bath to provide power to the temperature controller The controller will display both the current red and set green temperature Baths should not be powered on for more than a few seconds without buffer in the bath inserts Do not heat dry baths Do not touch the bottom metal surface of the bath during operation Insert the bath liner and fill with buffer before turning on power to each bath A This symbol indicates the potential presence of a Hot Surface Turn off the temperature controller and leave sufficient time for cooling or use heat protection prior to handling a bath Insert liner and add liquid before heating 2 To change the set temperature press the up or down arrow buttons until the desired temperature is shown Allow time for the bath to reach the set temperature and stabilize Refer
3. March 2013 Identifying System Components Figs 4 and 5 Pd ee N N N N N N E RP RP BP RP RP RP RP PRP H Mm A U N e O HO 0 N D UV HBP WN KF O Main power switch Turns on power to the unit Bath power switch Turns on power to each bath for heating liquid Temperature controller Used to set and observe bath temperature Temperature sensor Transmits bath temperature to controller Standard bath Removable insert compatible with most liquids solvents Low volume bath Removable stainless steel insert Gripper arm Rotates and moves slide racks up and down in baths Gripper Opens closes and secures the slide rack assembly Fan Draws air through the instrument cabinet for cooling Emergency switch Cuts power for safety Slide rack s Holds slides during processing Centrifuge access panel Automatically opens for the gripper to insert a slide rack Centrifuge latch Keeps lid closed Centrifuge lock Internal lock for safety Touchscreen cable connection Receptacle for attaching control cable Touchscreen Used to create store and run protocols and for calibration Pause button Toggle switch that stops and restarts a running protocol Centrifuge swinging buckets Green sample and red balance rack buckets Temperature calibration jack Receptacle for cable to thermometer Power cord connection Receptacle for power cord Stir bar speed control Rotating
4. Use the handheld touchscreen to navigate between screens select and run protocols create new protocols and control instrument movement Fig 17 If the touchscreen fails to respond it may require calibration See Section VI C for details Fig 17 Use touchscreen controls Fig 18 Pause protocols as needed C Using the Pause Button The action of the instrument can be immediately stopped and restarted using the illuminated red PAUSE RESUME button at top right on the touchscreen When pressed the instrument will stop and the button light will flash To resume the protocol press the button again Fig 18 D Using the Emergency Stop To immediately stop movement of the gripper arm and stir bars press the red Emergency Stop EMO button on the right side of the instrument Fig 19 Bath heaters will continue to receive power To restore power to the gripper arm and stir bars release the EMO button by rotating it a quarter turn clockwise Fig 20 To move the arm press Control Panel on the Home Page and use the up down left right buttons To remove the slide rack hold the rack and press the Open Gripper button on the Control Panel screen The protocol will not resume upon releasing the EMO switch and must be restarted from the beginning If the touch screen does not illuminate when the unit is turned on rotate the red EMO button on the side of the instrument to release the emergency stop Fig 19 Press EMO t
5. Provided in Shipment INSTRUMENT Carton ACCESSORIES Carton Varies by configuration March 2013 6 LD for FISH manual 2 0 software 1 4 for FISH SC G e Nn e Little Dipper Processor for FISH USER MANUAL System Components 7 Gripper arm SES 8 Gripper Ae 9 Fan in back not shown 11 Slide rack 12 Centrifuge access panel Bop i IR 13 Centrifuge latch rr iy lt a ibn MS CIO 11a 12 slide rack fixed handle L 11b 24 slide rack removable handle 5 Standard bath da SES Sa sich y N ouchscreen cable 21 Stir bar speed control V Tid a CNAGGHON 19 Temperature calibration jack im er di FA 2 Bath power switch a 23a Bath cover 3 Temperature controller 4 Temperature sensor 14 Centrifuge lock internal 1 Main power switch 20 Power cord connection e 23b Slotted bath cover 16 Touchscreen 17 Pause button Fig 4 Little Dipper Processor System Components Internal View of Centrifuge 11b 24 slide rack 24b Spacer for 24 slide rack 25b Absorbent pad large 18b Swinging bucket large 11a 12 slide rack 24a Spacer for 12 slide rack 25a Absorbent pad small 18a Swinging bucket small 13 Centrifuge latch Fig 5 Internal View of Centrifuge March 2013 7 LD for FISH manual 2 0 software 1 4 for FISH SC G e N e Little Dipper Processor for FISH USER MANUAL B
6. and TOUCHSCREEN Car b080 95 0 Onpas packaging materni F loam pieces aviirumoni Bag cale be TO GALORE EVERY USE 3 Manta sw mere nor NS LAUC TIONS i aphes t 1 Toro bagi boeg Pa re omme reed ie due page towe to remove tufo 7 Place the tsan rectarigie UB ron original pahaging atop the Bath 4 on oh vateh E Costi tower Ae gripper arm by peira Conn tom top rear atie Biting tron urdrrnazit the Anni utd i resis on the fam sncrangie E Place the U shaped parce of foam O eer the tae of the groper arm ta r un Fig 50 Repackaging instructions Aree PURA Tat aoe OOT ETEC TD mmn Fig 49 Maintenance instructions March 2013 23 LD for FISH manual 2 0 software 1 4 for FISH SC G e n e Little Dipper Processor for FISH USER MANUAL XI SERVICE A Lockout Procedure If a malfunction or need for service occurs comply with the following lockout procedure for restricting use of the instrument All users upon observing that the instrument is locked out for servicing or maintenance shall not attempt to start energize or use the equipment 1 Notify all affected users that servicing or maintenance is required and that the instrument must be shut down and locked out to perform the servicing or maintenance 2 Ifthe machine is operating shut it down by the normal stopping procedure turn off baths and main power or press the EMO switch 3 Disconnect the power cord from the wall sock
7. 1 Ensure baths racks and stir bars are free of buffer residue and dust See section VI A Cleaning Before Every Use 2 Insert baths and stir bars as specified for the protocol Fig 23 3 Rotate all temperature sensors down Fig 24 Fig 23 Place baths and stir bars Fig 25 Turn on main power Fig 26 Set temperature s Add recommended buffer s to the fill line Turn on main power to the instrument Fig 25 Turn on power to bath s to be heated and set temperature s Fig 26 Cover any baths set above 70 C Allow time for baths to reach set temperature s 7 Place a balance rack into the centrifuge with same number of slides as the sample rack Add sample slides to rack Remove any bath covers 10 Submerge the slide rack in Bath 1 between the two black lines 11 On the touchscreen home page press Run Protocol then select the desired protocol and press Start Now Fig 27 March 2013 13 LD for FISH manual 2 0 software 1 4 for FISH SC G e n e Little Dipper Processor for FISH USER MANUAL 12 Carefully place slide rack onto gripper Fig 28 The instrument will perform the steps as programmed 13 At completion varies by protocol remove the rack from the centrifuge Fig 29 or from above the last bath Fig 30 if running a protocol without centrifugation by pushing the thumbpad to open the gripper paddle 14 Clean the instrument per section VI B Cleaning After Every Use
8. FO CCC errate 24 Bi Fuse ReplacemMent ear 24 XII DECLARATION OFEONFORMITY il aa 25 March 2013 2 LD for FISH manual 2 0 software 1 4 for FISH SC G e N e Little Dipper Processor for FISH USER MANUAL I SAFETY NOTICES A Instrument Safety Before operating the instrument read the information in this section concerning hazards and potential hazards Ensure that everyone involved with the instrument s operation is instructed in both general safety practices for laboratories and specific safety practices for the instrument B Conventions and Labels The following is a glossary of the safety conventions and symbols that are used on the Little Dipper Processor Whenever such symbols appear on instruments please observe appropriate safety measures 1 Warnings Failure to comply with the following warnings that are affixed to the product can lead to possible personal injury or death For the continued HAZARDOUS protection against risk VOLTAGE of fire replace only with fuse of the specified type and current ratings Disconnect power before servicing Do not use in vicinity of n flammable or combustible Avoid spills on materials cabinet Fire or explosion may result This symbol indicates the potential for a Pinch Point Do not place fingers in the slot in the Z axis tower This symbol indicates the potential presence of a Hot Surface Turn off the temperature controller and leave sufficient tim
9. Little Dipper Processor for FISH USER MANUAL CAT 1080 70 1 115V 1080 70 2 230V FOR RESEARCH USE ONLY Not for Use in Diagnostic Procedures SciGene e 470 Lakeside Dr Ste F Sunnyvale CA 94085 USA e 408 733 7337 e techserv scigene com SC G e N e Little Dipper Processor for FISH USER MANUAL Serial Number The following serial number identifies the specific instrument you have purchased and must be referenced when requesting service A copy is affixed to the instrument Warranty The Little Dipper Processor described in this manual is warranted to be free of defects in materials and workmanship for a period of 12 months from date of delivery This warranty does not cover removable parts or accessories including removable baths In the event of a defect during the warranty period liability is limited to factory repair service at no charge All original packaging should be retained by the customer in the event that the instrument is returned to the factory for service or repair The foregoing warranty is void in the event the unit was abused or modified or used in a manner inconsistent with its intended purpose No other warranty is expressed or implied including warranties of merchantability and fitness for a particular purpose In no event shall SciGene or its representatives be liable for any direct indirect special incidental or consequential damages or for any damages resulting from loss arising out of or in connecti
10. ack on the open gripper use the left right and up down arrows on the touchscreen to position the rack in the center of the bath above the rotating stir bar such that it does not touch the rotating stir bar When finished remove the rack from the gripper and click Save Exit to save settings CALIBRATE BATH 1 LOADING CENTER SLIDERACK IN BATH 2 Ce Cn CANCEL Fig 43 Calibrate Bath 1 Loading screen Fig 44 Center slide rack in Bath 2 screen CANCEL 2 Bath 2 Center Calibration March 2013 Insert standard baths into Baths 1 and 2 with stir bars Activate stir bars Hold a 12 position rack in Bath 1 aligned with the black lines on the instrument and slightly below the fill line Using the touchscreen press Control Panel Advanced Settings Bath 2 Center Fig 44 The gripper arm will move over Bath 1 open and lower Load the slide rack handle onto the paddle bumps on the gripper as it closes Let go of the rack The gripper arm will move the rack to Bath 2 and stop Verify that the rack is centered in Bath 2 Use the left right arrows to adjust the position if necessary Press Save Exit when finished 20 LD for FISH manual 2 0 software 1 4 for FISH SC G e n e Little Dipper Processor for FISH USER MANUAL C Centrifuge Loading Calibration This test ensures the rack is automatically inserted into the sample bucket of the centrifuge 1 Hold a 12 position rack in Bath 1 In the Advanced Settin
11. ch ON power ON Fuse has blown Replace fuse with same type Instrument is receiving power but robot will not respond Pause button has been Press the red pause button on the top of the touch Protocol does not run activated screen Bath temperature varies by several degrees from NIST certified thermometer Emergency stop button has Twist the red EMO button on the side of the been activated instrument to release the emergency stop Temperature controller needs Follow the instructions in section VII A Temperature to be re calibrated Controller Calibration Touchscreen does not Follow the instructions in section VII A Touchscreen Touchscreen needs calibration respond Calibration Question Answer How do I clean the Refer to section VI C Periodic Maintenance and or request a Little Dipper Periodic instrument Cleaning and Maintenance document from your SciGene representative Fig 49 How do I safely move the Request a Packing the Little Dipper Processor for Shipment document Fig X 2 from instrument your SciGene representative Fig 50 SciGene sciGene Little Dipper Processor Packing the Little Dipper Processor for Shipment Periodic Cleaning and Maintenance aa Lathe Dipper insirsment cleaned Cried and Cerontarmmated per Cere procedure Rutta bocketi whee cocks Aptal thermometer or other accermoriei ar applicable v pedine dna Can Original bosek INSTRUMENT LO ACCISSORMS
12. d racks with running tap water then rinse in DI water and ethanol and dry with a lint free towel Store in clean plastic bags to protect from dust Fig 41 2 Clean temperature sensors with DI water and lint free towel 3 Wipe exterior of instrument cabinet with a damp paper towel Fig 40 Clean temperature sensors Fig 41 Place clean baths in bags Monthly Cleaning and Calibration 1 Calibrate bath temperatures using a NIST certified thermometer See section VI A Temperature Controller Calibration 2 Remove and wash the centrifuge buckets and spacers using warm water and lint free towels 3 Replace the absorbent pad in each centrifuge bucket Clean all exterior surfaces of the cabinet gripper paddles and gripper arm housing with damp lint free towels 5 Remove the centrifuge buckets Clean the inside surfaces of the centrifuge and rotor with damp lint free towels 6 Inspect the centrifuge buckets for signs of wear or deformation where they hang from the rotor Replace if worn or bent 18 LD for FISH manual 2 0 software 1 4 for FISH SC G e N e Little Dipper Processor for FISH USER MANUAL VII SYSTEM CALIBRATION The instrument is calibrated and tested at the factory and should not require adjustment under most circumstances z E Actual Temperature Set Temperature A Temperature Controller Calibration left display red right display green The EZ Zone controllers are calibrated at the facto
13. e for cooling or use heat protection prior to handling a bath Biological hazards can be associated with the materials used on this instrument Potentially infectious waste and other hazardous waste must be treated in accordance with good laboratory practice guidelines March 2013 3 LD for FISH manual 2 0 software 1 4 for FISH SC G e N e Little Dipper Processor for FISH USER MANUAL 2 Cautions Failure to comply with the following cautionary statement affixed to the product may lead to possible personal injury Do not touch the bottom metal surface of the bath during operation Insert the bath liner and fill with buffer before turning on power to each bath Insert liner and add liquid before heating The Little Dipper System weighs approximately 59 Ibs 26 5 kg Use caution when lifting the unit to avoid personal injury Do not under any circumstances lift the unit by the cylindrical tower or its extended gripper arm This will damage the instrument Do not under any circumstances run a protocol while a bath cover is in place To achieve temperatures above 70 C a bath cover must be used to obtain and maintain temperatures When the robot is in use baths running at high temperatures may need to be topped off due to evaporation C Spills and Reagent Disposal Observe all national regional and local regulations for waste disposal and management Biological and other hazards can be associated with the mater
14. ep Input screen Select the remaining parameters for Step 1 of the protocol and then enter the desired values within the ranges indicated Table 2 Parameter Settings for Custom Protocols Parameter Range 1 Time the instrument pauses at the end of the step with rack remaining submerged 2 After agitation time that the rack is suspended above bath before moving 16 LD for FISH manual 2 0 software 1 4 for FISH SC G e Nn e Little Dipper Processor for FISH USER MANUAL 6 To adda pause within the protocol do one of the following e Enter time for rack to remain submerged at end of step e Enter 9999 for User Control Rack will stay submerged until the operator presses the touchscreen to resume the protocol This is beneficial for protocols that require more than 5 baths When all values for Step 1 are entered press Save and Exit to return to the Step Input screen Continue to enter values for additional steps max 9 Fig 36 Remember to program C for the centrifuge if ending in a drying step When finished creating the protocol press Save amp Exit to return to the Select Protocol screen Up to 3 steps are displayed at one time on the touch screen Each screen of the protocol must be saved separately before saving the entire protocol B Deleting a Protocol March 2013 1 To delete a protocol press Control Panel from the home screen then Advanced Settings Press the Delete Protocol button t
15. esentative Place of Issue Director of Product Manufacturing June 17 2010 Title of Authorized Representative __ 7 Dateofissue 1 Pt Signature of Authorized Representative 25 LD for FISH manual 2 0 software 1 4 for FISH
16. et and from the instrument so that the machine is isolated from energy sources Fig 51 Twist and remove the fuse Fig 52 Place in a locked safe Contact your SciGene representative to arrange for service Fig 51 Disconnect power cord Fig 52 Remove fuse B Fuse Replacement March 2013 The fuse may be replaced by turning off and unplugging the unit then gently twisting and removing the old fuse Replace with a new fuse slow blow with the same electrical rating 250V 20A for 115V or 250V 10A for 230V 24 LD for FISH manual 2 0 software 1 4 for FISH SC G e N e Little Dipper Processor for FISH USER MANUAL XII March 2013 DECLARATION OF CONFORMITY Little Dipper Processor Model 650c SciGene 470 Lakeside Dr Ste F Sunnyvale CA 94085 4720 USA Declares that the above referenced product s meets the essential requirements of the following European Union Directives by using the relevant standards shown below to indicate compliance EMC Directive 2004 108 EC EN 61326 1 2006 Electrical equipment for measurement control and laboratory use to include EN 61000 4 2 1995 A1 1998 A2 2001 EN 61000 4 3 2006 EN 61000 4 4 2004 EN 61000 4 6 2007 LVD Directive 2006 95 EC EN 60204 1 2006 Electrical equipment of machines EN 61010 1 2004 Safety requirements for measurement control and laboratory use 2 Edition Part 1 General requirements Terry Gill Sunnyvale California USA Name of Authorized Repr
17. gs screen press C fuge Loading Fig 45 Load the rack onto the gripper The gripper will close and then move the rack to the centrifuge Fig 46 The centrifuge access panel opens automatically and the gripper arm lowers the rack inside stopping at a set position in the green bucket 2 Look down through the access panel to verify that the rack is centered from side to side in the bucket Use the arrow buttons to adjust the position of the arm and centrifuge rotor if needed 3 Press Save Exit The arm will move up and down in the centrifuge and then release the rack Make sure the rack gently falls into place into the green bucket CALIBRATE CENTRIFUGE LOADING CENTRIFUGE CANCEL y Fig 45 Calibrate Centrifuge Loading screen Fig 46 Rack loading into centrifuge D Touch Screen Calibration If the touchscreen fails to respond to touch it may need calibration 1 To access the Touchscreen Calibration screen press the touchscreen while the instrument is powering up Touch point 1 Fig 47 On the Point 2 screen touch point 2 On the Point 3 screen touch point 3 Fig 48 After the Home screen displays check that the screen now responds to touch If it does not respond contact your SciGene representative de E e POINT 1 Entering Calibration Mode Please remove finder from touchscreen and touch the following Points POINT 3 Fig 47 Touchscreen calibration point 1 Fig 48 Touchscreen calibration
18. ials used on this instrument Potentially infectious and other hazardous waste materials must be treated in accordance with good laboratory practices This symbol indicates the potential presence of a Hot Surface Use care when cleaning a spill in this area to avoid being burned 1 Cleaning Spills a Turn off the instrument b Wipe the spill using an absorbent material Always pull the liquid to the front of the instrument away from the control panel and around the bath positions c Clean the deck with a cloth or paper towel and an appropriate disinfectant solution e g 70 ethanol for the type of spill present d Wipe the deck dry Dispose of the material in a manner that is in accordance with good laboratory practices 2 Disposing of Reagents Some chemicals used on this instrument can be hazardous Please adhere to good laboratory practices when disposing of hazardous chemicals March 2013 4 LD for FISH manual 2 0 software 1 4 for FISH SC G e N e Little Dipper Processor for FISH USER MANUAL Il SITE PREPARATION The Little Dipper Processor for FISH requires a level surface area of at least 30 x 22 inches 76x56cm with a vertical clearance of 30 inches 76 cm Fig 1 Locate the unit within 3 feet 1meter of a single power outlet that delivers at least 1700 Watts 20 Amps of constant power Clear the benchtop before unpacking any cartons Allow unimpeded access to the Emergency Off EMO and main power swi
19. main power 9 LD for FISH manual 2 0 software 1 4 for FISH SC G e n e Little Dipper Processor for FISH USER MANUAL IV SYSTEM OPERATION A Installing Baths and Centrifuge Buckets 1 Clean baths racks and stir bars by rinsing in running tap water followed by 100 ml of DI water and then 100 ml of ethanol Fig 10 Dry with a lint free towel Clean sensors with DI water and a lint free towel Fig 11 Install standard or heatable low volume baths by rotating temperature sensors up placing baths and then lowering sensors Fig 12 For low volume baths without temperature control leave sensors down while placing baths 4 Place a stir bar in all baths Fig 13 Low volume baths use low profile stir bars SciGene cat 1080 11 1 Fig 11 Clean sensors Fig 13 Add stir bars Fig 15 Insert buckets Fig 16 Insert pads and spacers Unlock and open the centrifuge Fig 14 Hang a red bucket on the rotator arms tabs marked BALANCE RACK Repeat using a green bucket on the rotator arms marked SAMPLE RACK Fig 15 7 Place an absorbent pad in each bucket with a black frame spacer on top Fig 16 Balance the centrifuge by inserting a rack with the same number of slides as the sample rack into the red bucket 9 Close and lock the centrifuge March 2013 10 LD for FISH manual 2 0 software 1 4 for FISH SC G e n e Little Dipper Processor for FISH USER MANUAL B Using the Touch Screen
20. o open the screen Choose a protocol to delete using the up and down arrows and then press Select A confirmation screen appears saying Delete Protocol Choose YES to confirm Press the BACK button on each of the previous screens to return to the home screen ADVANCED SETTINGS MENU ADVANCED SETTINGS MENU CALIBRATIONS BATH 1 BATH 2 OADING CENTER C FUGE OADING CALIBRATIONS BATH 1 BATH 2 ORDING CENTER C7 FUGE QADING Fig 37 Delete Protocol button DELETE PROTOCOL Use Arrow Keys to Select Protocol gt gt Quik tst DELETE PROTOCOL BlueGi SELECT Fig 38 Delete Protocol screen Fig 39 Delete Protocol confirmation 17 LD for FISH manual 2 0 software 1 4 for FISH SC G e n e Little Dipper Processor for FISH USER MANUAL VI A March 2013 PERIODIC MAINTENANCE Cleaning Before Every Use Baths racks and stir bars must be free of buffer residue and dust before processing slides If they were not cleaned and stored properly after the last use wash and dry them as follows Fill baths containing the rack s and stir bars with tap water Wipe racks stir bars and baths with a lint free towel Rinse in running tap water Rinse with approximately 100 200 ml of DI water Rinse with 100 ml of ethanol and dry with a lint free towel ee ee PS Clean temperature sensors with DI water and a lint free towel Fig 40 Cleaning After Every Use 1 Wash baths stir bars an
21. o stop Fig 20 Rotate to restore power March 2013 11 LD for FISH manual 2 0 software 1 4 for FISH SC G e N e Little Dipper Processor for FISH USER MANUAL E Runninga Test Protocol Practicing Rack Loading This test verifies the mechanical operation of the instrument and provides the operator an opportunity to practice loading the slide rack onto the gripper An empty slide rack is automatically moved from bath to bath with agitation followed by a brief centrifugation No liquid is required and temperature controllers should remain OFF TE Fig 21 Quik tst protocol Fig 22 Load slide rack 1 Ensure that baths and buckets are properly installed see section IV A Installing Baths and Centrifuge Buckets and correspond to the size of the slide racks to be used Verify that the all temperature sensors are rotated down Turn on main power located on the right side of the instrument Using the touchscreen press Run Protocol from the home screen then select the Quik tst protocol Fig 21 4 Hold an empty slide rack centered in Bath 1 aligned with the black lines on the instrument and slightly below the bath fill line 5 Startthe protocol by pressing Start Now on the touchscreen The gripper arm will lower into Bath 1 with the gripper open Align the holes in the handle with the two bumps in the gripper paddle as the gripper closes Fig 22 If the gripper closes and the rack is not held securely press the red EMO
22. on with the sale use or performance of the product Copyright Copyright 2005 2013 SciGene Corporation All rights reserved SciGene and Little Dipper are trademarks of SciGene Corporation Sunnyvale California USA All other trademarks used in this manual are the property of their respective owners Intended Use The intended use of this instrument is for washing and drying of fluorescent in situ hybridization FISH slides or microarrays This instrument should only be used according to instructions provided in this User Manual and other SciGene technical documents If the equipment is used in a manner not specified by the manufacturer the protection provided by the equipment may be impaired March 2013 1 LD for FISH manual 2 0 software 1 4 for FISH SC G e N e Little Dipper Processor for FISH USER MANUAL Table of Contents Is SAFETY NOTICES wien cin aaa iaia 3 A INSEFURI PACS sr ara 3 Bi Connessi peer 3 C Spills and Reagent DISPOSAL caasatace sccacsanednmatioanssnncansatecudasssncastionatuncieneivenY sanctamadieatenntanedeceairanacuseivenTenadusstes 4 il SME PREPARATION ea aiar 5 II UNPACKING BND SETUP ricicusecsevencccrctcnccsiensiccenesececageonnccinceretoasowsauevancsensstueeetssucesdesanessnmestesenansseseuteavess 6 A Unpacking and Verifying ItemS 6 B Identifying System COMPOMENIES sincicaoceosiscnncaveesnticasesmesdnoueonpsasecuvanrseecudsammdaaeu ant 8 C Computer and Power ConnectionS
23. osition slide rack Each 1080 20 1 Slide Rack 12 Position for standard 3 inch slides Includes handle Each 1080 20 0 Slide Rack 24 Position for standard 3 inch slides without handle Each 1080 20 5 Slide Rack 24 Position for standard 3 inch slides Includes handle Each 1080 24 0 Handle for Hybex or 24 position racks Each 1080 25 0 24 Slide Rack Kit Includes two 24 slide racks buckets red green spacers and pads Each 1080 21 0 Absorbent Pads for Slide Rack Buckets Hybex and 24 position 25 pk 25 PK 1080 21 1 Absorbent Pads for Slide Rack Buckets 12 Position 25 pk 25 PK 1080 23 1 Slide Rack Bucket for Samples green 12 position racks Each 1080 23 2 Slide Rack Bucket for Balance red 12 position racks Each 1080 23 3 Spacer for Rack Bucket 2 pk 12 position racks PK 1080 22 1 Slide Rack Bucket for Samples green Hybex and 24 position racks Each 1080 22 2 Slide Rack Bucket for Balance red Hybex and 24 position racks Each 1080 22 3 Spacer for Rack Bucket 2 pk Hybex and 24 position racks 2 PK 1080 41 0 Little Dipper Upgrade Kit for Agilent Arrays Each March 2013 22 LD for FISH manual 2 0 software 1 4 for FISH SC G e n e Little Dipper Processor for FISH USER MANUAL X TROUBLESHOOTING Indication Probable Cause Corrective Action Power disconnected Check power connections Instrument does not z Main power switch is OFF Turn main power swit
24. point 3 March 2013 21 LD for FISH manual 2 0 software 1 4 for FISH SC G e n e Little Dipper Processor for FISH USER MANUAL VIII SPECIFICATIONS Electrical 115V AC 50 60 Hz 1700W 20 Amps cat 1080 70 1 230V AC 50 60 Hz 1700W 10 Amps cat 1080 70 2 Contro Slide capacity Centrifuge speed Bath volumes i W k p gt Power supply Processing Standard 670 ml Low volume Low volume 210 ml heatable 270ml Operation 15 to 32 C 58 to 90 F Storage 20 to 60 C 4 to 140 F Relative humidity Operation 20 to 80 RH non condensing Storage 10 to 90 RH non condensing Overvoltage category Il Physical Dimensions 51 x 71 x 56 cm 20 x 28 x 22 inches H x W x D Weight net 26 5 kg 59 Ibs IX ORDERING INFORMATION Environmental Ambient temperature SciGene Cat Product Description UOM 1080 10 1 Standard Bath 670 ml Each 1080 10 2 Low Volume Bath 210 ml Includes a glass insert and stir bar Each 1080 10 5 Low Volume Heatable Bath 275 ml Includes a glass insert and stir bar Each 1080 11 0 Stir bars for Little Dipper standard baths 10 pk 10 PK 1080 11 1 Stir bars for Little Dipper low volume baths 10 pk 10 PK 1080 12 0 Bath cover Each 1080 12 1 Bath cover 5 pk 5 PK 1080 12 2 Bath cover slotted for Little Dipper 12 position slide rack Each 1080 12 4 Bath cover slotted for Little Dipper 24 p
25. r and thermometer when the unit was last calibrated 9 Use the up or down arrow to adjust the offset value to the temperature difference calculated in Step 4 For example if the controller shows a temperature 1 5 C lower than the thermometer adjust the offset by adding 1 5 to the value shown 10 Press the Infinity Key twice to exit calibration and return to the operation display Verify that the temperature on the thermometer matches the display The controller is now calibrated to provide accurate temperatures from 35 to 95 C March 2013 19 LD for FISH manual 2 0 software 1 4 for FISH SC G e N e Little Dipper Processor for FISH USER MANUAL B Slide Rack Position Calibration The instrument is calibrated and tested at the factory for proper rack positioning and should not require adjustment unless a new touchscreen is installed The following tests check proper alignment Baths should remain OFF with no liquid needed 1 Bath 1 Loading a b C Insert standard baths into Baths 1 and 2 Insert and activate stir bars Hold a 12 position rack in Bath 1 aligned with the black lines on the instrument and slightly below the fill line Using the touchscreen press Control Panel Advanced Settings Bath 1 Loading Fig 43 The gripper arm will move over Bath 1 open and lower Align the slide rack handle onto the paddle bumps on the open gripper The gripper will not close While continuing to hold the r
26. ry to provide accurate bath temperatures from ambient 5 to 95 C Fig 42 Re calibration is recommended only if the display varies by more than one degree 1 C to the Advance Button Up Down Arrows temperature shown on an externally connected NIST Infinity Return Key certified digital thermometer Cat 1051 52 0 Fia 42 EZ Zone Controller To adjust a controller to achieve accurate temperatures 1 Addwaterto bath fill line in a standard bath and ensure temperature sensor is down in contact with liquid in the bath Turn ON the instrument Turn on the temperature controller and set the temperature on the controller to 65 C Allow 30 minutes to stabilize 4 Using the cable provided with the digital thermometer plug one end into the blue receptacle found above the controller 5 Turnon the thermometer Allow 1 minute for thermometer to stabilize The actual temperature of the bath will be displayed 6 Calculate the difference between the temperature shown on the controller and on the digital thermometer For example if the controller displays 65 0 C and the thermometer displays 66 5 C the difference offset is 1 5 C 7 Onthe controller press the up and down arrows simultaneously for 3 seconds The left display shows A1 and the right display shows open 8 Press the Advance Button green circle 3 times until the right display shows i CA The left display shows the offset value between the controlle
27. switch immediately Fig 19 Release the rack if needed by pressing Open Gripper from the Control Panel screen then select and start the protocol again Immediately press the EMO switch to stop the instrument if the slide rack fails to mount on the gripper properly or touches the sides of the bath The instrument will now agitate the rack in each bath Observe placement of the slide rack in the baths The rack will automatically move from the last bath into the centrifuge and spin for a minute If the rack touches the bath see VII B Slide Rack Position Calibration When the protocol is complete unlock and open the centrifuge lid to remove the rack from the sample bucket March 2013 12 LD for FISH manual 2 0 software 1 4 for FISH SC G e n e Little Dipper Processor for FISH USER MANUAL F Running a Pre Installed Protocol The instrument comes configured with validated protocols installed Refer to the Little Dipper Processor Method corresponding to your protocol for details on how to set up the instrument Method sheets can be found online at www scigene com A Do not under any circumstances run a protocol while a bath cover is in place To achieve and maintain temperatures above 70 C a bath cover must be used while the machine is NOT in motion Baths at high temperatures may need buffer s added to offset evaporation The following are general guidelines on how to set up and run a pre installed protocol
28. switch to adjust stir bar speed Stir bar Creates a gentle vortex in the bath Bath covers Lids help achieve and maintain temperatures above 70 C Slide rack spacer Raises rack above absorbent pad Absorbent pad Soaks up excess water in centrifuge 8 LD for FISH manual 2 0 software 1 4 for FISH SC G e n e Little Dipper Processor for FISH USER MANUAL C Computer and Power Connections March 2013 1 Connect the braided cable to the touchscreen and to the receptacle labeled TOUCHSCREEN on the right side of the instrument using the circular connectors Fig 6 Connect the power cord to the receptacle labeled POWER on the right side of the instrument Fig 7 and to a power source that delivers 15 Amps or more for 115V units or 8 Amps or more for 230V units Avoid connecting to a circuit with a centrifuge freezer or other equipment that draws significant power Turn on the main power switch on the right side of the instrument The touchscreen should illuminate and the motors should activate Fig 8 Briefly turn on power to each of the five baths using the power switch located above each bath position Fig 9 Verify that each temperature controller illuminates and then turn off power to each bath position Baths should not be powered on for more than a few seconds without buffer in the bath inserts Do not heat dry baths Fig 6 Connect touchscreen Fig 8 Turn on
29. tches on the right side of the instrument CES EG LUI 94 SAYDUI DE 30 inches 76 cm Figure 1 Allow proper clearance for the instrument March 2013 5 LD for FISH manual 2 0 software 1 4 for FISH SC G e Nn e Little Dipper Processor for FISH USER MANUAL III UNPACKING AND SETUP A Unpacking and Verifying Items The Little Dipper Processor instrument weighs about 59 Ibs 26 5 kg and is generally shipped in a large carton with an additional small carton for accessories A A Do not lift the unit by the robot arm Always grip the instrument from underneath the base when lifting Never attempt to lift or move the instrument alone Use caution when lifting the unit to protect yourself and others from personal injury Open the large carton and remove the interior box and foam 2 With the aid of another person carefully lift the instrument by its base only and place it directly on the benchtop Fig 2 Ensure clearance around the instrument making sure not to block the cooling fan in the back 3 Use scissors to cut the white tie wrap that prevents the gripper arm from rotating during shipping Fig 3 4 Unpack the interior box that contains the touchscreen power cord and user documentation Open the smaller carton to remove the accessories and additional components Verify all packing list items are received in good condition Figure 2 Lift by the base Figure 3 Cut tie wrap Table 1 Items
30. to section VII A Temperature Controller Calibration for information on calibrating the controllers Red WATLOW EZ ZONE Fig 31 Temperature controllers Fig 32 Set temperature March 2013 15 LD for FISH manual 2 0 software 1 4 for FISH SC G e n e Little Dipper Processor for FISH USER MANUAL CUSTOM PROTOCOLS V A March 2013 Adding a Protocol Custom protocols can be created using the touchscreen The touchscreen can store up to 14 protocols with 1 to 9 steps each L 4 From the touchscreen Home page press New Edit Protocol On the Create Edit Protocol screen Fig 33 use the up down buttons to move the cursor to NEW Press Select to go to the Name Protocol screen Fig 34 pn Within the Name Protocol screen use the shift key to move the up arrow within the numeric 10 key pad until the desired letter is indicated Press to select the indicated letter displayed above the key pad Continue for up to eight characters When finished press Enter to go to the Step Input screen Fig 35 and then press Step 1 When the Parameters screen displays press the Bath button enter 1 and then press Enter A All protocols start from Bath 1 5 t FRU Ui Key to navigate le gt characters ma TOGO 4 PETE a Fig 33 Create Edit Protocol screen STEP INPUT SIEP BATH AGITATE l sPeed secs stroke IHRE f bal NAME amp E Fig 35 St
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