Fiberlite F13-14x50cy Rotor User Manual
Contents
1. POLYESTER GLASS THERMOSET Pyridine 5096 Rubidium Bromide Rubidium Chloride Sucrose naj nm a co POLYSULFONE Sucrose Alkaline co a CC Co PET POLYCLEAR CLEARCRIMP CCCLEARCRIMP Sulfosalicylic Acid Nitric Acid 1096 nA nm CC oi Gol Col ANODIC COATING for ALUMINUM mM A Gol Gol CO Gol Gol GLASS Gol a m oi TYGON Gol Col Gol Col Gol Gol Col Col co Nitric Acid 5096 Nitric Acid 9596 Hydrochloric Acid 1096 Hydrochloric Acid 5096 Sulfuric Acid 1096 ol ol ol oi oi ol NEOPRENE eco Gol Gol ee co co H o n ol oc ol oi ol ol oi ol oi VTN Sulfuric Acid 5090 lt c C c ccc C Go Sulfuric Acid Stearic Acid Lo Go Gol Go Tetrahydrofuran Toluene c aj z c c c c c aj a c z Z Z o vjl el sei oli ol oi oli ol aj Gol c oi c z Trichloroacetic Acid zi Z ol c DERI c Z Z Z Trichloroethane c c c c zm nm wm a ol Gol ccc c2. IMPORTANT Please reference this page for the most up to date information on the following Web site addresses e contact information copyright and trademark information All subsequent pages in this manual may have incorrect web site addresses and contact information 2013 Thermo Fisher Scientific Inc All rights reserved HERAEUS is a registered trademark of Heraeus Holding GmbH licensed to Thermo Fisher Scientific Delrin TEFLON and Viton are registered trademarks of DuPont Noryl is a registered trademark of SABIC POLYCLEAR is a registered trademark of Hongye CO Ltd Hypaque is a registered trademark of Amersham Health As RULON A and Tygon are registered trademarks of Saint Gobain Performance Plastics Alconox is a registered trademark of Alconox Ficoll is a registered trademark of GE Healthcare Haemo Sol is a registered trademark of Haemo Sol Triton X 100 is a registered trademark of Sigma Aldrich Co LLC All other trademarks are the property of Thermo Fisher Scientific Inc and its subsidiaries Manufacturer Thermo Fisher Scientific Robert Bosch StraBe 1 D 63505 Langenselbold Germany Thermo Fisher Scientific Inc provides this document to its customers with a product purchase to use in the product operation This document is copyright protected and any reproduction of the whole or any part of this document is strictly prohibited except with the written authorization of Thermo Fisher Scientific Inc The
3. ol Zn Si Gol wm Gol z a wn Z a a a wm ol c POLRTHYLNE Gol Gol Gol wm sl co Zn z Gol Gol c a Z oi oi oi a GW Col POLYPROPYLENE Gol co A YM Gol Gol Gol Gol rz c SI Gol Gol ol ol TITANIUM c c c c z c c co c oi ni nl nl ol Gol ol ol oi ol oi ol nin Lol C CT Mm DA WD WM Col Col Mm oi co Ethylene Glycol B 2 75003661 F13 14x50c Thermo Scientific CHEMICAL Ethylene Oxide Vapor MATERIAL ANODIC COATING for ALUMINUM CELLULOSE ACETATE BUTYRATE POLYURETHANE ROTOR PAINT DELRIN ETHYLENE PROPYLENE NORYL B Chemical Compatibility Chart POLYCLEAR CLEARCRIMP CCCLEARCRIMP POLYESTER GLASS THERMOSET POLYTHERMIDE Ficoll Hypaque co a COMPOSITE Carbon Fiber Epoxy Gol GLASS co TYGON Hydrofluoric Acid 1096 Hydrofluoric Acid 5096 N N a POLYRTHYLENE co POLYSULFONE Hydrochloric Acid Formaldehyde 40 oi gt a c POLYVINYL CHLORIDE Gol Gol a a RULONA TEFLON Glutaraldehyde oo Glycerol Guanidine Hydrochloride Haemo Sol
4. POLYVINYL CHLORIDE c c c c c Go c c c C Z c c oi oi o WM oi CC BUNAN Cl aj a c c c c c c aj aj oi c a NYLON c c o c aj C c c aj aj aj oi a POLYCARBONATE c c C c c Z Z a Gol oi Gol oi SILICONE RUBBER Gol 0 Go Co wm Gol Co C gt Trichloroethylene Trisodium Phosphate 5 Tris Buffer neutral pH co Triton X 1009 Urea Co 1096 Hydrogen Peroxide 396 Xylene Cl Gol ol oi c Gol Gol z SI oi aj ol ol Z ol POLYALOMER al ol Gol nA Gol Cl ol ol oi c z oi ol oi a oi a POLYRTHYLENE A Gol Gol c oi c ol zm a oi oi a POLYPROPYLENE Gol Gol Gol Al Al Al Gol Gol Gol Gol Gol Gol Gol Gol Gol Gol Gol Gol Gol Gol Gol Gol roi Gol RULONA TEFLON a c oi c oi oi Gol oi aj TITANIUM Cl ol z c Gol nln Gol nln ol c z an nl ale i i ni ol ol Gol ei ol ol oi ol nl oi ec ol oi ol oi el ol ol
5. Hexane Z Si ALUMINUM nN WM Z Gol Gol Gol Gol Col co Z Gol Z STAINLESS STEEL cl mn Co Isobutyl Alcohol Isopropyl Alcohol lodoacetic Acid Potassium Bromide Potassium Carbonate co Potassium Chloride Potassium Hydroxide 596 Gol Col Gol Col Col co Gol Col Gol nM Col co MA NA Gol Gol AL Gol Gol Gol CO Gol Gol oi c oi Gol NYLON Zin nm ol Gol Gol Gol c coc SIICONERUBBER Potassium Hydroxide Potassium Permanganate oi Gol Calcium Chloride C co Z co z co NM A MA MND DA WD WN co Calcium Hypochlorite Kerosene A Gol Gol z oi wm oi oi c Cl ol NEOPRENE Loi Col TI NDNA A A A NA A NA NA YN co Sodium Chloride 10 Sodium Chloride sat d Mm ccs co 1 zZz nn Carbon Tetrachloride Mm Col Col WM Col co Mm Col zio o z z c c c z aj aj C Z c c Aqua Regia Go gt CT Gol Z Co 1 1 1 1 Solution 555 2096 Magnesium Chloride Mercaptoacetic Acid Therm
6. ol ol Gol ol ol Gol ol o sel ol ol Zinc Chloride Thermo Scientific 75003661 F13 14x50c B 5 B Chemical Compatibility Chart Zinc Sulfate USS 59 9 5 5 5 5 5 5 5 9 5 5 888855555 Citric Acid 1096 MS 5 5 SMS 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 9 Polyethylencterephthalate S Satisfactory M attack may be satisfactory for use in centrifuge depending on length of exposure speed involved etc suggest testing under actual conditions of use U Unsatisfactory not recommended Performance unknown suggest testing using sample to avoid loss of valuable material Chemical resistance data is included only as a guide to product use Because no organized chemical resistance data exists for materials under the stress of centrifugation when in doubt we recommend pretesting sample lots B 6 75003661 F13 14x50c Thermo Scientific Warranty and recommended Service Life Recommended Warranty Years Retirement After Years FIBERLite F13 14x50c 15 Recommended Retirement Rotor Type Warranty After Years O ringe tubes bottles 30 Days 1 Thermo Fisher Scientific warrants that the Products will operate substantially in conformity with Thermo Fisher Scientific s published specifications when subjected to normal proper and intended usage by properly trained personnel for a period of 5 YEARS after shipment to the Customer
7. the Warranty Period Thermo Fisher Scientific agrees during the Warranty Period provided it is promptly notified in writing upon the discovery of any material defect and further provided that all costs of returning the defective Goods to Thermo Fisher Scientific are pre paid by the Customer to repair or replace at Thermo Fisher Scientific s option defective Goods so as to cause the same to oper ate in substantial conformance with the said specifications Replacement parts may be new or refur bished at the election of Thermo Fisher Scientific All replaced parts shall become the property of Thermo Fisher Scientific Thermo Fisher Scientific s sole liability with respect to equipment materials parts or software furnished to Thermo Fisher Scientific by its third party suppliers shall be limited to the assignment by Thermo Fisher Scientific to the Customer of any such third party supplier s war ranty to the extent the same is assignable In no event shall Thermo Fisher Scientific have any obliga tion to make repairs replacements or corrections required in whole or in part as the result of i normal wear and tear ii accident disaster or event of force majeure iii misuse fault or negligence of or by or on behalf of the Customer iv use of the Goods in a manner for which they were not designed v causes external to the Goods such as but not limited to power failure or electrical power surges or vi use of the Goods in co
8. 15 3 8 3 15 3 Pitch angle 34 34 34 Accel braking time s 145 90 145 90 120 105 sample heating at nmax C referred to ambient 19 19 21 temperature of 23 C running time 60 minutes aerosol tight no no no max temperature for autoclaving 121 121 121 Table 1 4 120V 60Hz refrigerated Centrifuge Heraeus Multifuge X3R Heraeus Multifuge X3FR Heraeus Multifuge X1R Catalog 75004516 75004537 75004251 Weight empty kg of rotor 7 16 7 16 7 16 Maximum permissible load g 14x75 14x75 14x75 maximum speed nmax rpm 9 250 9 250 8 500 maximum RCF value at nmax 14 636 14 636 12 359 max min radius 8 3 15 3 8 3 15 3 8 3 15 3 Pitch angle 34 34 34 Accel braking time s 135 90 135 90 115 105 aerosol tight no no no max temperature for autoclaving C 121 121 121 Thermo Scientific 75003661 13 14 50 1 3 1 Rotor Specifications Sorvall Sorvall Table 1 5 230V 50Hz 60Hz ventilated Centrifuge Sorvall Legend XT Sorvall Legend XF Sorvall Legend X1 Catalog 75004505 75004532 75004220 Weight empty kg of rotor 7 16 7 16 7 16 Maximum permissible load g 14x75 14x75 14x75 maximum speed rpm 10 000 10 000 8 500 maximum RCF value at nmax 17 105 17 105 12 359 max min radius 8 3 15 3 8 3 15 3 8 3 15 3 Pitch angle 34 34 34 Accel braking time s 135 90 135 90 95 90 sample heating at Nmax C referred to 21 21 19 ambi
9. 4100 8 3 15 3 1560 2875 4200 8 3 15 3 1637 3017 4300 8 3 15 3 1716 3163 4400 8 3 15 3 1796 3312 4500 8 3 15 3 1879 3464 4600 8 3 15 3 1964 3620 4700 8 3 15 3 2050 3779 4800 8 3 15 3 2138 3941 4900 8 3 15 3 2228 4107 5000 8 3 15 3 2320 4276 5100 8 3 15 3 2414 4449 5200 8 3 15 3 2509 4625 5300 8 3 15 3 2607 4805 5400 8 3 15 3 2706 4988 5500 8 3 15 3 2807 5174 5600 8 3 15 3 2910 5364 5700 8 3 15 3 3015 5558 5800 8 3 15 3 3122 5754 5900 8 3 15 3 3230 5954 6000 8 3 15 3 3341 6158 6100 8 3 15 3 3453 6365 6200 8 3 15 3 3567 6575 6300 8 3 15 3 3683 6789 6400 8 3 15 3 3801 7006 6500 8 3 15 3 3921 7221 6600 8 3 15 3 4042 7451 6700 8 3 15 3 4166 7679 6800 8 3 15 3 4291 7910 75003661 F13 14x50c Thermo Scientific RCF Values Speed rpm UD n RCF RCF 6900 8 3 15 3 4418 8144 7000 8 3 15 3 4547 8382 7100 8 3 15 3 4678 8623 7200 8 3 15 3 4810 8867 7300 8 3 15 3 4945 9115 7400 8 3 15 3 5081 9367 7500 8 3 15 3 5220 9622 7600 8 3 15 3 5360 9880 7700 8 3 15 3 5502 10142 7800 8 3 15 3 5646 10407 7900 8 3 15 3 5 91 10675 8000 8 3 15 3 5939 10947 8100 8 3 15 3 6088 11223 8200 8 3 15 3 6239 11502 8300 8 3 15 3 6393 11784 8400 8 3 15 3 6548 12070 8500 8 3 15 3 6704 12359 8600 8 3 15 3 6863 12651 8700 8 3 15 3 7024 12947 8800 8 3 15 3 7186 13246 8900 8 3 15 3 7350 13549 9000 8 3 15 3 7516 13855 9100 8 3 15 3 7684 14165 9200 8 3 15 3 7854
10. 6000 358 9 329 10200 Countries not listed 49 6184 90 6000 Part of Thermo Fisher Scientific Table of Contents Prelat mme E iii Scope of Supply aa cda uio iii PREC AMNION e iii Chapter1 Rotor Specifications 1 1 cr EE 1 2 230V SONG DUE z ventilated 1 2 230V 50Hz 60Hz refrigerated Las eer hos ries Beca Senden ER er Pr nici cape 1 2 eadera rii T oer 1 3 1 3 e E 1 4 230V EE 1 4 230V 50Hz 60Hz refrigerated eege 1 4 120V 60Hz ventilated EE 1 5 120V Ni ieoi r 1 5 100V 50Hz 60FHz ventilat d E 1 6 100V 50H2 60Hz ere 1 6 RT EC WE 2 1 STEET 3 1 Rotor Installati n H 3 2 Removing thi ogc stats Ehe 3 3 Chapter4 Rotor Loading 4 1 Before RUN Med PE T 4 2 Etage 4 2 Improper Loading eR ku reb POR MERE dH eR er di 4 3 Maimun EPI PT T 4 3 Chapterb Aerosol tight Applications ee 5 1 Basie E 5 2 EE ee 5 2 Checking the Aerosol Tightness i duns DR abren ce 5 2 Chapter6 Maintenance and Care 22222 6 1 Cleaning Titer valle M 6 2 EE Ee 6 2 Disinfection E 6 3 Decontamination ates soon eege E er te
11. approved cleansers If in doubt contact Thermo Fisher Scientific When cleaning centrifug Use warm water with a neutral solvent Never use caustic cleaning agents such as soap suds phosphoric acid bleaching solutions or scrubbing powder Rinse the cavities out thoroughly Use a soft brush without metal bristles to remove stubborn residue Afterwards rinse with distilled water Place the rotors on a plastic grate with their cavities pointing down If drying boxes are used the temperature must never exceed 50 C since higher temperatures could damage the material and shorten the lifetime of the parts Use only disinfectants with a pH of 6 8 Dry aluminum parts off with a soft cloth After cleaning treat the entire surface of aluminum parts with corrosion protection oil 7000 9824 Also treat the cavities with oil Store the aluminum parts at room temperature or in a cold storage room with the cavities pointing down CAUTION Before using any cleaning or decontamination methods except those recommended by the manufacturer users should check with the manufacturer that the proposed method will not damage the equipment 6 2 75003661 F13 14x50c Thermo Scientific 6 Maintenance and Care Disinfection Clean centrifuge and accessories as follows l A YH N Open the centrifuge Turn off the centrifuge Pull out the power supply plug Grasp the rotor with both hands and lift it vertically off the c
12. nete RR 6 4 PA DD Me 6 5 Thermo Scientific 75003661 F13 14x50c i Contents 75003661 F13 14x50c Service of Thermo Fisher 6 5 Lil DID quem M A 1 Chemical Compatibility Chart 222 2222 2 B 1 Warranty and recommended Service Life C 1 Contact Te gt 0 1 Thermo Scientific pP Preface Before starting to use the rotor read through these instruction manual carefully and follow the instructions The information contained in these instruction manuals is the property of Thermo Fisher Scientific it is forbidden to copy or pass on this information without explicit approval Failure to follow the instructions and safety information in this instruction manual will result in the expiration of the seller s warranty Scope of Supply Article Number Quantity Check 75003661 FIBER Lite F13 14x50c 1 76003500 Rubber seal grease 1 70009824 Anti corrosion oil 1 L 50120007 Instruction Manual 1 If any parts missing please contact the nearest Thermo Fisher Scientific representative Precautions In order to ensure safe operation of the FIBERLite 77 F13 14x50c the following general safety regulations must be followed Do not remove the magnet at the rotor bottom Do not use rotors which show any signs of corrosion and or cracks Useonly with rotors that ha
13. 0x115 Included 25 PP Sealing Tc 366036 15 15 Nunc Conical 50 17x120 Included 50 PP Sealing 75100378 2 1 2 2 75003661 F13 14x50c Thermo Scientific AutoLock Contents Rotor Installation on page 3 2 Removing the Rotor on page 3 3 Thermo Scientific 75003661 F13 14x50c 3 1 3 AutoLock Rotor Installation Rotor Installation CAUTION Unapproved or incorrectly combined accessories can cause serious damage to the centrifuge This rotor is equipped with an AutoLock system This system is used to automatically lock the rotor to the centrifuge spindle The rotor does not have to be bolted onto the centrifuge spindle Proceed as follows 1 Open the lid of the centrifuge and if necessary remove any dust foreign objects or residue from the chamber AutoLock and o ring must be clean and undamaged AutoLock O ring Figure 1 AutoLock 2 Hold the rotor over the centrifuge spindle and let it slide slowly down the centrifuge spindle The rotor clicks automatically into place CAUTION Do not force the rotor onto the centrifuge spindle If the rotor is very light then it may be necessary to press it onto the centrifuge spindle with a small amount of pressure 3 Check if the rotor is properly installed by lifting slightly on the handle If the rotor can be pulled up then it must be reclamped to the centrifuge spindle WARNUNG If the rotor cannot be properly locked in place afte
14. 120V 60Hz refrigerated Centrifuge Sorvall Legend XTR Sorvall Legend XFR Sorvall Legend X1R Catalog 75004521 75004539 75004261 Weight empty kg of rotor 7 16 7 16 7 16 Maximum permissible load 9 14x75 14x75 14x75 maximum speed Nmax rpm 9 250 9 250 8 500 maximum value at nmax 14 636 14 636 12 359 max min radius 8 3 15 3 8 3 15 3 8 3 15 3 Pitch angle 34 34 34 Accel braking time s 135 90 135 90 115 105 aerosol tight no no no max temperature for autoclaving C 121 121 121 75003661 13 14 50 1 5 1 Rotor Specifications Sorvall Table 1 9 100V 50Hz 60Hz ventilated Centrifuge Sorvall Legend XT Sorvall Legend XF Sorvall Legend X1 Catalog 75004507 75004534 75004223 Weight empty kg of rotor 1 16 1 16 7 16 Maximum permissible load g 14x75 14x75 14x75 maximum speed nmax rpm 9 250 9 250 8 500 maximum value at nmax 14 636 14 636 12 358 max radius 8 3 15 3 8 3 15 3 8 3 15 3 Pitch angle 34 34 34 Accel braking time s 150 90 150 90 120 105 sample heating at Nmax C referred to 18 18 21 ambient temperature of 23 C running time 60 minutes aerosol tight no no no max temperature for autoclaving 121 121 121 Table 1 10 100V 50Hz 60Hz refrigerated Centrifuge Sorvall Legend XTR Sorvall Legend XFR Sorvall Legend X1R Catalog 75004522 75004540 75004263 Weight empty kg of rotor 7 16 7 16
15. 14478 9300 8 3 15 3 8026 14794 9400 8 3 15 3 8199 15114 9500 8 3 15 3 8375 15438 9600 8 3 15 3 8552 15764 9700 8 3 15 3 8 31 16094 9800 8 3 15 3 8912 16428 9900 8 3 15 3 9095 16765 10000 8 3 15 3 9279 17105 Thermo Scientific 75003661 F13 14x50c A 3 Chemical Compatibility Chart lt POLYCLEAR CLEARCRIMP CCCLEARCRIMP 22 REE y 2 SZ e Bn 8 Se al ee CHEMICAL BE E LEE 2 mercaptoethanol S S y SMS S USS S S S SS 500 55 9 8 85 Acetaldehyde S U U MUUUMM MS U S U Acetone MSUUSUM S UUS US UUUS 5 UUS MMS UU Acetonitrile SSU 5 5 5 5 US UMUU S MUUS 5 5 5 Alconox UUS SSS Allyl Alcohol U S 5 5 5 5 5 MS Aluminum Chloride 1158585 US 5 5 5 5 5 5 5 5 5 85358 5 5 Formic Acid 10096 S MU U U SMUUSS U S U S U Ammonium Acetate S S H S S S 55525 57555505 Ammonium Carbonate MSUSSSSSSSSSSSUU SSSSSSMSESSS Ammonium Hydroxide 10 UUSUSSMSSSSS 5 5 5 5 5 5 5 5 5 8 Ammonium Hydroxide 2896 HASS H MS 6 9 5 MS Ammonium Hydroxide UUUUSUMS 5 5050 0555 MS SSS U Ammonium Phosphate U 5 S SSSSSSS SSM SSSSSSMSSS Ammonium Sulfate UMS 58 US 3955 85 35 8 9 SSSSSSUSSU Alcohol S MU S S M S 5 5 5 5 U S M Aniline 55 UUS US MS UUUUUUU 5 MUUS 5 5 5 US Sodium Hydroxide lt 1 U SMS S SMMS 5 5 5
16. 14x75 14x75 14x75 maximum speed rpm 10 000 10 000 8 500 maximum RCF value at nmax 17 105 17 105 12 359 max min radius 8 3 15 3 8 3 15 3 8 3 15 3 Pitch angle 34 34 34 Accel braking time s 135 90 135 90 95 90 sample heating at Nmax C referred to 21 21 19 ambient temperature of 23 C running time 60 minutes aerosol tight no no no max temperature for autoclaving C 121 121 121 Table 1 2 230V 50Hz 60Hz refrigerated Centrifuge Heraeus Multifuge X3R Heraeus Multifuge X3FR Heraeus Multifuge X1R Catalog 75004515 75004536 75004250 Weight empty kg of rotor 7 16 7 16 7 16 Maximum permissible load g 14x75 14x75 14x75 maximum speed Nmax rpm 10 000 10 000 8 500 maximum value at nmax 17 105 17 105 12 358 max min radius cm 8 3 15 3 8 3 15 3 8 3 15 3 Pitch angle 34 34 34 Accel braking time s 115 90 115 90 95 90 aerosol tight no no no max temperature for autoclaving C 121 121 121 1 2 75003661 F13 14x50c Thermo Scientific Table 1 3 120V 60Hz ventilated 1 Rotor Specifications Heraeus Centrifuge Heraeus Multifuge X3 Heraeus Multifuge X3F Heraeus Multifuge X1 Catalog 75004501 75004531 75004211 Weight empty kg of rotor 7 16 7 16 7 16 Maximum permissible load g 14x75 14x75 14x75 maximum speed nmax rpm 9 250 9 250 8 500 maximum value at nmax 14 636 14 636 12 359 max min radius cm 8 3 15 3 8 3
17. 5 5 5 5 U Sodium Hydroxide 1096 U MU U 5 5 5 5 5 5 5 5 5 5 5 U Barium Salts MUS 9 9 5 5 5 Benzene SS8UuUuusuMUuUuSuusuuuMUuMuuusuusus Thermo Scientific 75003661 F13 14x50c B 1 B Chemical Compatibility Chart CHEMICAL ANODIC COATING for ALUMINUM COMPOSITE Carbon Fiber Epoxy POLYURETHANE ROTOR PAINT MATERIAL GLASS NORYL POLYSULFONE STAINLESS STEEL TYGON Benzyl Alcohol cc CELLULOSE ACETATE BUTYRATE Boric Acid lt PET POLYCLEAR CLEARCRIMP CCCLEARCRIMP lt POLYESTER GLASS THERMOSET zz ETHYLENE PROPYLENE Cl c POLYTHERMIDE Cesium Acetate Cesium Bromide Cesium Chloride Cesium Formate Cesium lodide Cesium Sulfate Chloroform 00 Col Col co ol ol a oi SILICONE RUBBER MAI Col MDA Gol Gol WD Col co Chromic Acid 1096 c aj mM Mm zz NEOPRENE Gol Col Gol Gol Gol Gol Col Col co MN lt co WD Col Col ND WD gt Col ND WD gt Chromic Acid 50 Cresol Mixture Cyclohexane Deoxycholate Distilled Water Dextran C1 MN WM WM WM WM WM WM WM WN WN c c c z zi Col Col nA WD Col Col co MAI Gol Gol Gol Col co Gol Gol Gol Gol co Gol nA Gol Go
18. 7 16 Maximum permissible load g 14x75 14x75 14x75 maximum speed Nmax rpm 9 000 9 000 8 500 maximum value at nmax 13 855 13 855 12 358 max min radius cm 8 3 15 3 8 3 15 3 8 3 15 3 Pitch angle 34 34 34 Accel braking time s 120 80 120 80 115 105 aerosol tight no no no max temperature for autoclaving C 121 121 121 1 6 75003661 F13 14x50c Thermo Scientific Contents e Rotor data on page 2 1 e Accessories on page 2 1 Thermo Scientific 75003661 F13 14x50c 2 1 2 Accessories Thermo Scientific General Purpose Rotors F13 14x50c Carbon Fiber Rotor F13 14x50c Fixed Angle Carbon Fiber Rotor Rotor Specifications Capacity ml 14 x 50 conical Tube Dimensions mm 30 x 121 Angle 34 Maximum Speed rpm 10000 K Factor 1547 Net Weight kg 76 Max Speed may vary depending on centrifuge model RCF Radius RCF x g Radius cm Maximum 17 105 15 3 Minimum 9 271 8 3 F13 14x50c Carbon Fiber Rotor Cat No Description 75003661 F13 14x50c Carbon Fiber Rotor Cat No Description 099 149027 Replacement Lid Assembly 021 149027 O ring Refresher Kit Tube Ordering Information Sealing Assembly Required Adapters Required Accessories Required Tube Vol Max Dims xL Cat No Vol Fill Description Qty Speed mm ea Qty insani TNT Qty of rpm at NO mea at No Places 334959 50 50 Nunc Conical 25 10 000 3
19. 9 100 Russia 7 812 703 42 15 Thermo Scientific 75003661 F13 14x50c D 1 0 Contact Information Spain Portugal Switzerland UK Ireland China 34 932 23 09 18 34 932 23 09 18 41 44 454 12 12 44 870 609 9203 86 21 6865 4588 India Japan Other Asian Countries Latin America Other Countries 86 10 8419 3588 91 22 6716 2200 81 45 453 9220 852 2885 4613 1 866 984 3766 49 6184 90 6940 D 2 75003661 F13 14x50c 33 2 2803 2180 Thermo Scientific Index A 0 JACUBSSOHBS Ud dui M UR I C RR 2 1 5 2 Aerosol tight Applications 5 1 AMT 6 5 GE A 1 B Removing the Rotor 3 3 Basie E 5 2 Rotor Installation Ate 3 2 Before Bul adeunt nta e 4 2 Rotor E 4 Rotor Specifications 1 1 6 1 5 Checking the Aerosol Tightness 5 2 Geelen iii Nes TI ac 6 2 Service TECNICA 6 5 Cleaning agents me 6 3 Sorvall 1 4 D 1 0 Wafranty em C 1 Decontamination E 6 4 Disinfection eege 6 3 F IINE Meet 5 2 H e 1 2 E 4 3 M NANOS M 6 1 P PR iii Preface ee iii Proper Loading geseet 4 2 Thermo Scientific 75003661 F13 14x50c 1 1 Part of Thermo Fisher Scientific SCIENTIFIC
20. bonate 296 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 8 9 5 9 9 5 5 9 5 Sodium Dodecyl Sulfate 5 5 5 555 5 5 5 5 5 5 9 5 5 5 5 5 5 5 5 5 9 5 Sodium Hypochlorite 596 5 5 5 5 5 SMS 5 5 5 MS 5 5 MUS MS Sodium lodide MS 5 5 5 5 5 5 5 5 5 5 9 5 5 5 5 5 5 MS 5 5 Sodium Nitrate S 5 5 5 9 5 5 9 5 5 9 bb 9 9 9 9 5 5 5 5 5 Sodium Sulfate USS 58 5 5 9 9 5 9 9 5 5 9 853585 9 5 9 SMS 5 5 Sodium Sulfide 5 55 5 55 5 UU 5 S SM S Sodium Sulfite 5 5 5 SSSSMSSSSSSM 5 5 5 5 5 5 5 5 5 9 Nickel Salts US 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 MS 5 5 Oils Petroleum SSS SUSSSSUUMSMUUSSSUSSSS Oils Other S S SMS 5 5 5 5 5 5 5 US 5 5 5 5 5 5 Oleic Acid S USSSUUSUSSMSSSSSSSSSMUSMM Oxalic Acid 5 5 5 5 5 5 5 5 5 4 5 5 5 5 5 5 5 5 5 5 Perchloric Acid 10 U U SUU SMM MUMS MM MS U S S Perchloric Acid 70 U U U U U SUMUUMUUUMMUMSUUSUS Phenol 596 USU SMM SUMUUSUMSMSUUSUMMMS S Phenol 50 USU SUM SUMUUUUUSUMUUSUUUMS Phosphoric Acid 10 UUMS 5 5 5 5 5 5 4 SSSSSSSSSUMUSS Phosphoric Acid UUMM U S MSUUMMSSSMSMSUMU S B 4 75003661 F13 14x50c Thermo Scientific B Chemical Compatibility Chart CHEMICAL POLYURETHANE ROTOR PAINT COMPOSITE Carbon Fiber Epoxy ETHYLENE PROPYLENE MATERIAL Z Z Si ccc coc zm zm Z Z AUMINUM CELLULOSE ACETATE BUTYRATE Physiologic Media Serum Urine Picric Acid co Co POLYTHERMIDE
21. contents of this document are subject to change without notice All technical information in this document is for reference purposes only System configurations and specifications in this document supersede all previous information received by the purchaser Thermo Fisher Scientific Inc makes no representations that this document is complete accurate or error free and assumes no responsibility and will not be liable for any errors omissions damage or loss that might result from any use of this document even if the information in the document is followed properly This document is not part of any sales contract between Thermo Fisher Scientific Inc and a purchaser This document shall in no way govern or modify any Terms and Conditions of Sale which Terms and Conditions of Sale shall govern all conflicting information between the two documents thermoscientific com Australia 61 39757 4300 India toll free 1800 22 8374 Russia 7 812 703 42 15 Austria 43 1 801 400 India 91 22 6716 2200 Spain Portugal 34 03 223 09 18 Belgium 32 53 73 42 41 Italy 32 02 95059 552 Switzerland 41 44 454 12 22 China 800 810 5118 or Japan 81 3 5826 1616 UK Ireland 44 870 609 9203 er m 400 650 5118 Netherlands 31 76 579 55 55 USA Canada 1 866 984 3766 France 33 2 2803 2180 New Zealand 64 9 980 6700 SCIENTIFIC Germany national toll free 0800 1 536 376 Nordic Baltic CIS countries Other Asian countries 852 2885 4613 Germany international 49 6184 90
22. e AutoLock 4 Check the rotor s suitability using the chemical compatibility chart on page B 1 5 Make sure the tubes or bottles do not touch the lid Proper Loading To ensure safe operation of the centrifuge the rotor must be evenly loaded at all times 4 2 75003661 F13 14x50c Thermo Scientific 4 Rotor Loading Improper Loading Improper Loading Maximum Loading Thermo Scientific The rotor can run at high speeds The rotor design has sufficient reserve stability even when spinning at top speed The safety system of the centrifuge requires that you symetrically balance the tubes in the rotor This refers to each cavity There are two options available for centrifuging samples whose weight including adaptor exceeds the maximum permissible load Reduce the fill level Reduce the speed Use this formular Maximum permissible load idum e Effective load admissible speed n maximum speed 75003661 F13 14x50c 4 3 Aerosol tight Applications Thermo Scientific 75003661 F13 14x50c 5 1 5 Aerosol tight Applications Basic Principles Basic Principles CAUTION When centrifuging hazardous samples do not open aerosol tight rotors or buckets unless placed in a safety cabinet Always bear in mind the maximum permitted fill levels Be sure to check all seals before starting any aeorsol tight applications Check that the sample containers are well suited for the desired centr
23. e rotor first with ethanol and then with de ionized water Adhere strictly to the given application times Be sure the decontamination solution can drain off the rotor Rinse the rotor and accessories thoroughly with water Dispose of the decontamination solution according to the applicable guidelines Dry all of the rotors and accessorie after cleaning with a cloth or in a warm air cabinet at a maximum temperature of 50 After cleaning treat the entire surface of aluminum parts with corrosion protection oil 7000 9824 Also treat the cavities with oil Tread the boldof the swing out rotor with bold grease 75003786 Before autoclaving clean rotor and accessories and described above Place the rotor on a flat surface Rotors and adapter can beautoclaved at 121 The maximum permissible autoclave cycle is 20 minutes at 121 Clean the rotor before autoclaving and rinse it with distilled water Remove all accessories tubes adapters from the rotor Place the rotor on a flat surface Note No chemical additives are permitted in the steam CAUTION Never exceed the permitted temperature and duration when autoclaving If the rotor shows signs of corrosion or wear it must be replaced Service of Thermo Fisher Scientific Thermo Fisher Scientific recommends having the centrifuge and accessories serviced once a year by an authorized service technician The service technicians check the following the
24. electrical equipment the suitability of the set up site the lid lock and the safety system the rotor the fixation of the rotor and the drive shaft Thermo Fisher Scientific offers inspection and service contracts for this work Thermo Scientific 75003661 F13 14x50c 6 5 LLL EB RCF Values Speed rpm RCF RCF 300 8 3 15 3 8 15 400 8 3 15 3 15 27 500 8 3 15 3 23 43 600 8 3 15 3 33 62 700 8 3 15 3 45 84 800 8 3 15 3 59 109 900 8 3 15 3 75 139 1000 8 3 15 3 93 171 1100 8 3 15 3 112 207 1200 8 3 15 3 134 246 1300 8 3 15 3 157 289 1400 8 3 15 3 182 335 1500 8 3 15 3 209 385 1600 8 3 15 3 238 438 1700 8 3 15 3 268 494 1800 8 3 15 3 301 554 1900 8 3 15 3 335 618 2000 8 3 15 3 371 684 2100 8 3 15 3 409 754 2200 8 3 15 3 449 828 2300 8 3 15 3 491 905 2400 8 3 15 3 534 985 2500 8 3 15 3 580 1069 2600 8 3 15 3 627 1156 2700 8 3 15 3 676 1247 2800 8 3 15 3 728 1341 2900 8 3 15 3 780 1439 3000 8 3 15 3 835 1539 3100 8 3 15 3 892 1644 Thermo Scientific 75003661 F13 14x50c 1 RCF Values 2 Speed rpm GER RCF Rmin RCF Rmax 3200 8 3 15 3 950 1752 3300 8 3 15 3 1011 1863 3400 8 3 15 3 1073 1977 3500 8 3 15 3 1137 2095 3600 8 3 15 3 1203 2217 3700 8 3 15 3 1270 2342 3800 8 3 15 3 1340 2470 3900 8 3 15 3 1411 2602 4000 8 3 15 3 1485 2737
25. ent temperature of 23 C running time 60 minutes aerosol tight no no no max temperature for autoclaving 121 121 121 Table 1 6 230V 50Hz 60Hz refrigerated Centrifuge Sorvall Legend XTR Sorvall Legend XFR Sorvall Legend X1R Catalog 75004520 75004538 75004260 Weight empty kg of rotor 146 146 1 16 Maximum permissible load g 14x75 14x75 14x75 maximum speed Nmax rpm 10 000 10 000 8 500 maximum value at nmax 17 105 17 105 12 358 max min radius cm 8 3 15 3 8 3 15 3 8 3 15 3 Pitch angle 34 34 34 Accel braking time s 115 90 115 90 95 90 aerosol tight no no no max temperature for autoclaving C 121 121 121 1 4 75003661 F13 14x50c Thermo Scientific 1 Rotor Specifications Thermo Scientific Sorvall Table 1 7 120V 60Hz ventilated Centrifuge Sorvall Legend XT Sorvall Legend XF Sorvall Legend X1 Catalog 75004506 75004533 75004221 Weight empty kg of rotor 1 16 1 16 1 16 Maximum permissible load g 14x75 14x75 14x75 maximum speed nmax rpm 9 250 9 250 8 500 maximum value at nmax 14 636 14 636 12 359 max min radius 8 3 15 3 8 3 15 3 8 3 15 3 Pitch angle 34 34 34 Accel braking time s 145 90 145 90 120 105 sample heating at Nmax C referred ambient 19 19 21 temperature of 23 C running time 60 minutes aerosol tight no no no max temperature for autoclaving 121 121 121 Table 1 8
26. entrifuge spindle Remove the centrifuge tubes and adaptors Usea neutral deaning agent with a pH value between 6 and 8 for cleaning Dry all of the rotors and accessorie after cleaning with a cloth or in a warm air cabinet at a maximum temperature of 50 Clean the housing of the centrifuge as needed After cleaning treat the entire surface of aluminum parts with corrosion protection oil 7000 9824 Also treat the cavities with oil Tread the boldof the swing out rotor with bold grease 75003786 CAUTION When cleaning do not allow liquids especially organic solvents to get on the drive shaft the bearings the AutoLock or the locks Organic solvents break down the grease in the motor bearing The drive shaft could freeze up After some applications their might be ice in the rotor chamber Let the ice melt and drain it off Clean the rotor chamber as described above Disinfection Disinfect the centrifuge immediately whenever infectious material has spilled during centrifugation WARNING Infectious material can get into the centrifuge when a tube breaks or as a result of spills Keep in mind the risk of infection when touching the rotor and take all necessary precautions In case of contamination make sure that others are not put at risk Decontaminate the affected parts immediately Take other precautions if need be Use a sprayer whenever possible so that all surfaces are covered evenly The rotor cha
27. ifugation process Fill Level The tubes are only to be filled to a level which ensures that the sample is unable to reach the top of the tube during centrifugation Therefore fill the tube only 2 3 of the rated level Checking the Aerosol Tightness The aerosol tightness testing of the rotors and buckets depend on the microbiological test process in accordance with the EN 61010 2 020 Appendix AA Whether or not a rotor is aerosol tight depends primarily on proper handling Check as needed to make sure your rotor is aerosol tight The careful inspection of the seals and seal surfaces for signs of wear and damage such as cracks scratches and embrittlement is extremely important Aerosol tight applications are not possible if the lids are open Aerosol tightness requires the correct operation when filling the sample vessels and closing the rotor lid Quick Test As a quick test it is possible to test the aerosol tightness of fixed angle rotors using the following process 1 Lubricate all seals lightly Always use the special grease 7600 3500 when lubricating the seals 2 Fill the cavities with approx 10 ml of carbonated mineral water 3 Close the rotor as explained in the handling instructions 4 Shake the rotor vigorously using your hands This releases the carbonic acid gas which is bound in the water resulting in excess pressure Do not apply pressure to the lid when doing so Leaks can be detected by escaping water
28. l co Gol Gol Gol Gol Go Diethyl Ether n Diethyl Ketone n n Diethylpyrocarbonate Dimethylsulfoxide c c c c nm wm Cl zz oi ol oi c POLYCARBONATE c Cl zc ol ol a cc c a POLYVINYL CHLORIDE nl Gol nl nl nl Gol ol nl ol ol ol RULONA TEFLON Dioxane c co Ferric Chloride Acetic Acid Glacial e lt 5 co Acetic Acid 6096 Ethyl Acetate Gol Cl ol ol Ethyl Alcohol 5096 c ol toi Mm DA Col CO nM Col CO Mm DA WM Col CO Ethyl Alcohol 95 C C C C C Z c Ethylene Dichloride A c Si Gol nM c c coo c c c z E Z ALUMINUM a c c c z c c c c co ol c c c ajaj aj oi ol a c BUNAN Z Z Z 2 2 Gol si an Gol DELRIN i ceo i i i i i i i AM ol Gol ol ol oi oi Cc oi oi oi a NYLON Gol cl nm cz sl Cl ol Zn nm wm c a aj SI oi oi Gol wm ol ol ol c POLYALOMER lt co Cl Gol
29. mber and the rotor should be treated preferably with a neutral disinfectant A disinfectant spray would be most suitable for this purpose so that the rotor and accessory surfaces are covered evenly CAUTION Before using any cleaning or decontamination methods except those recommended by the manufacturer users should check with the manufacturer that the proposed method will not damage the equipment Observe the safety precautions and handling instructions for the cleaning agents used Contact the Service Department of Thermo Fisher Scientific for questions regarding the use of other disinfectants Thermo Scientific 75003661 F13 14x50c 6 3 6 Maintenance and Care Decontamination Disinfect the rotor and accessories as follows 1 Open the centrifuge Turn off the centrifuge Pull out the power supply plug Grasp the rotor with both hands and lift it vertically off the centrifuge spindle Remove the centrifuge tubes and adaptors and dispose of them or disinfect them n A N Treat the rotor and accessories according to the instructions for the disinfectant spray or soak in solution Adhere strictly to the given application times 7 Be sure the disinfectant can drain off the rotor 8 Rinse the rotor and rotor lid thoroughly with water and then rub down 9 Dispose of the disinfectant according to the applicable guidelines 10 Dry all of the rotors and accessorie after cleaning with a cloth or in a warm air cabine
30. mbination with equipment or software not supplied by Thermo Fisher Scientific If Thermo Fisher Scientific determines that Goods for which the Customer has requested warranty services are not covered by the warranty hereunder the Customer shall pay or reim burse Thermo Fisher Scientific for all costs of investigating and responding to such request at Thermo Fisher Scientific s then prevailing time and material rates If Thermo Fisher Scientific provides repair services or replacement parts that are not covered by the warranty provided in this Clause the Cus tomer shall pay Thermo Fisher Scientific therefor at Thermo Fisher Scientific s then prevailing time and materials rates Any installation maintenance repair service relocation or alteration to or of or other tampering with the Goods performed by any person or entity other than Thermo Fisher Scien tific without Thermo Fisher Scientific s prior written approval or any use of replacement parts not sup plied by Thermo Fisher Scientific shall immediately void and cancel all warranties with respect to the affected Goods Thermo Scientific 75003661 F13 14x50c C 1 Contact Information United States 866 9 THERMO 1 866 984 3766 Canada 1 866 984 3766 Austria 43 1 801 400 Belgium 32 2 482 30 30 Germany 08001 536 376 49 6184 90 6940 France 33 2 2803 2180 33 2 2803 2000 Italy 39 02 02 95059 341 Netherlands 31 76 571 4440 Nordic Baltic Countries 35 89 32
31. o Scientific Gol Gol cz c c c BUNAN 1 1 1 1 1 1 1 1 1 1 1 1 co C co Gol Gol Cl Z Sn nA ol Gol Gol Gol YW ol ol POLYALOMER oi c coc Si POLYCARBONATE Gol nm cz ol Gol Gol MW c WM oi oi oi oi GW j co POLYPROPYLENE 75003661 13 14 50 zl nm oi c c ol TITANIUM B Chemical Compatibility Chart 2 E E E 2 2 d t 8 Methyl Alcohol 555 US 5 5 5 5 5 5 US UMS 5 5 5 5 5 5 MS MU Methylene Chloride UUuUUMSSUSUUSUUUUUMUUUSSMUSU Methyl Ethyl Ketone 55 UUS 5 MS 5 5 5 UU 5 5 0555550 0 Metrizamide MSS 555 5 5 5 5 5 S 5 5 5 5 5 5 MS 5 5 Lactic Acid 10096 5 MS U 5 5 5 MS 5 MS MS 5 5 Lactic Acid 2096 55 MS M 5 5 5 5 5 9 5 MS 5 5 5 N Butyl Alcohol S S U S 5 US MS 5 5 5 MMS 5 5 N Butyl Phthalate 55 SSS SUUSUUUM UUSUSMMSUS N N Dimethylformamide 5 5 5 US MS 5 5 US US UU 5 5 UUS MS 5 5 U Sodium Borate MS 5 5 5 5 5 5 5 5 5 155 5 59 58 5655 5 5 5 Sodium Bromide USS SSS 5 5 5 5 5 5 5 5 9 5565555 5 5 5 Sodium Car
32. or the sound of escaping gas 5 2 75003661 F13 14x50c Thermo Scientific 5 Aerosol tight Applications Checking the Aerosol Tightness Replace the seals if you detect any leaks Then repeat the test 5 Dry the rotor rotor lid and the cover seal CAUTION Prior to each use the seals in the rotor are to be inspected in order to assure that they are correctly seated and are not worn or damaged Damaged seals are to be replaced immediately When loading the rotor ensure that the rotor lid closes securely Damaged or clouded rotor covers are to be replaced immediately Thermo Scientific 75003661 F13 14x50c 5 3 5 Aerosol tight Applications Checking the Aerosol Tightness 5 4 75003661 F13 14x50c Thermo Scientific Maintenance and Care Thermo Scientific 75003661 F13 14x50c 6 1 B Maintenance and Care Cleaning Intervalls Cleaning Intervalls For the sake of personal environmental and material protection it is your duty to clean and if neces sary disinfect the centrifuge on a regular basis Maintenance Recommended interval Clean rotor chamber daily or when polluted Clean rotor daily or when polluted Accessories daily or when polluted Cabinet Once per month Ventilation holes Every six months Cleaning CAUTION Refrain from using any other cleaning or decontamination procedure than those recommended here if you are not entirely sure that the intended procedure is safe for the equipment Use only
33. r several attempts then the is defective and you not permitted to operate the rotor Check for any damage to the rotor damaged rotors must not be used Keep the hub area clear of objects 3 2 75003661 F13 14x50c Thermo Scientific 3 AutoLock Removing the Rotor CAUTION Check that the rotor is properly locked on the centrifuge spindle before each use by pulling it a its handle Be sure to check all seals before starting any aerosol tight applications 4 Close the centrifuge door Removing the Rotor To remove the rotor proceed as follows 1 Open the centrifuge door 2 Grab the rotor handle with both hands and press against the green AutoLock button At the same time pull the rotor directly upwards with both hands and remove it from the centrifuge spindle Make sure not to jam the rotor while doing this Thermo Scientific 75003661 F13 14x50c 3 3 4 Rotor Loading Rotor Loading Contents Before Run on page 4 2 Proper Loading on page 4 2 Improper Loading on page 4 3 Maximum Loading on page 4 3 Thermo Scientific 75003661 F13 14x50c 4 1 4 Rotor Loading Before Run Before Run 1 Please read and observe the safety instructions contained in these operating instructions and in the instructions for use 2 Check the rotor and all accessory parts for damages such as cracks scratches or traces of corrosion 3 Check the rotor chamber the centrifuge spindle and th
34. t at a maximum temperature of 50 After cleaning treat the entire surface of aluminum parts with corrosion protection oil 7000 9824 Also treat the cavities with oil Tread the boldof the swing out rotor with bold grease 75003786 Decontamination Decantaminate the centrifuge immediately whenever radioactive material has spilled during centrifugation WARNING Radioactive material can get into the centrifuge when a tube breaks or as a result of spills Keep in mind the risk of infection when touching the rotor and take all necessary precautions In case of contamination make sure that others are not put at risk Decontaminate the affected parts immediately Take other precautions if need be CAUTION Before using any cleaning or decontamination methods except those recommended by the manufacturer users should check with the manufacturer that the proposed method will not damage the equipment For general radioactive decontamination use a solution of equal parts of 7096 ethanol 1096 SDS and water Disinfect the rotor and accessories as follows 1 Open the centrifuge 2 Turn off the centrifuge 3 Pull out the power supply plug 4 Grasp the rotor with both hands and lift it vertically off the centrifuge spindle 6 4 75003661 F13 14x50c Thermo Scientific 10 Autoclaving 1 2 6 Maintenance and Care Autoclaving Remove the centrifuge tubes and adaptors and dispose of them or disinfect them Rinse th
35. ve been loaded properly Never overload the rotor Thermo Scientific 75003661 F13 14x50c iii Preface Use only accessories which have been approved by Thermo Fisher Scientific Exceptions to this rule are commercially available glass or plastic centrifuge tubes provided they have been approved for the speed or the RCF value of the rotor Please observe the safety instructions Please pay particular attention to the following aspects Rotor installation Check that the rotor is locked properly into place before operating the centrifuge Always balance the samples Maximum sample density at maximum speed 124 m This symbol refers to general hazards ATTENTION means that material damage could occur WARNING means that injuries or material damage or contamination could occur This symbol refers to biological hazards Observe the information contained in the instruction manual to keep yourself and your environment safe iv 75003661 F13 14x50c Thermo Scientific Rotor Specifications Contents Heraeus on page 1 2 e Sorvall on page 1 4 Thermo Scientific 75003661 F13 14x50c 1 1 1 Rotor Specifications Heraeus Heraeus Table 1 1 230V 50Hz 60Hz ventilated Centrifuge Heraeus Multifuge X3 Heraeus Multifuge X3F Heraeus Multifuge X1 Catalog 75004500 75004530 75004210 Weight empty kg of rotor 7 16 7 16 7 16 Maximum permissible load g
Download Pdf Manuals
Related Search