Sample & Assay Technologies QIAsafe® DNA Blood
Contents
1. Genomic DNA from whole blood stored in QlAsafe DNA Blood Tubes or 48 Well Plates can be recovered by following this protocol After rehydration the DNA can be purified using standard DNA purification protocols for whole blood We recommend using one of the protocols listed in Table 4 page 13 Things to do before starting E Pipet 4 4 ml Protease Resuspension Buffer into the vial containing lyophilized QIAGEN Protease as indicated on the label Dissolved QIAGEN Protease is stable for up to 2 months when stored at 2 8 C Storage at 20 C is recommended to prolong the life of QIAGEN Protease but repeated freezing and thawing should be avoided For this reason storage of aliquots of QIAGEN Protease is recommended E Prepare a rehydration solution by mixing 44 ul reconstituted QIAGEN Protease with 286 ul RNase free water per sample Use Table 3 as a guide Always prepare fresh rehydration solution Table 3 Preparing rehydration solution Volume of RNase free Volume of QIAGEN No of samples water pl Protease pl 286 44 12 3432 528 24 6864 1056 48 13 728 2112 Procedure 1 Add 300 ul rehydration solution see Table 3 directly to the dried blood in a QlAsafe DNA Blood Tube or a well of a QlAsafe DNA Blood 48 Well Plate Individual wells in a plate can be opened by puncturing the AirPore Tape Sheet with a pipette tip or razor blade 2 Place the plate or the tubes placed in the supplied tube carrier plate on a2. 159134 159142 Number of preps 50 10 x 48 QlAsafe DNA Blood Tubes 50 QlAsafe DNA Blood Tube Carrier Plate 1 QlAsafe DNA Blood 48 Well Plates 10 AirPore Tape Sheets 10 QlAsafe Desiccant 5 10 QlAsafe Moisture Barrier Bags 5 10 Handbook QlAsafe DNA Blood Rehydration Set 100 Catalog no 159155 Number of preps 100 QIAGEN Protease 1 vial Protease Resuspension Buffer 6 ml RNase Free Water 50 ml Product Sheet The QlAsafe DNA Blood Rehydration Set is not included with QlAsafe DNA Blood Tubes and 48 Well Plates and must be purchased separately Storage QlAsafe DNA Blood matrix is a novel formulation for safe stable and convenient storage of whole blood at room temperature 15 25 C Before use QlAsafe DNA Blood Tubes and 48 Well Plates should be stored dry in their original unopened packaging Upon application of blood QlAsafe DNA Blood Tubes and Plates need to be stored in an environment with low relative humidity for optimal product performance Therefore it is recommended to store tubes or plates in a moisture barrier bag including a desiccant packet provided For extended 4 QlAsafe DNA Blood Handbook 09 2009 storage times gt 6 months moisture barrier bags should be heat sealed Alternatively tubes or plates may be stored in a dry storage cabinet with relative humidity below 40 see Equipment and Reagents to Be Supplied by User page 9 Storage of opened QlAsafe DNA Blood Tubes and Pla
3. Mini Kit for isolation of genomic mitochondrial bacterial parasite or viral DNA QlAamp DNA Mini Kit For 50 DNA preps 50 QlAamp Mini 51304 50 Spin Columns QIAGEN Proteinase K Reagents Buffers Collection Tubes 2 ml QlAsate DNA Blood Handbook 09 2009 17 Product Contents Cat no QlAamp DNA Mini Kit For 250 DNA preps 250 QlAamp Mini 51306 250 Spin Columns QIAGEN Proteinase K Reagents Buffers Collection Tubes 2 ml EZ1 DNA Blood 200 pl Kit for automated purification of DNA from 1 6 samples or 1 14 samples per run EZ1 DNA Blood 200 ul For 48 preps Reagent Cartridges 951034 Kit 48 Blood 200 ul Disposable Filter Tips Disposable Tip Holders Sample Tubes 2 ml Elution Tubes 1 5 ml QlAsymphony DNA Kits for automated purification of DNA from 1 96 samples QlAsymphony DNA For 192 preps of 200 ul each Includes 931236 Mini Kit 192 2 reagent cartridges and enzyme racks and accessories Related products for DNA storage QlAsafe DNA Tubes and 96 Well Plates for room temperature storage of DNA QlAsafe DNA Tubes 50 QlAsafe DNA Tubes in moisture 159104 50 barrier foil packages QlAsafe DNA 96 Well 10 QlAsafe DNA 96 Well Plates in 159112 Plates 10 moisture barrier foil packages 10 QlAsafe Seals For up to date licensing information and product specific disclaimers see the respective QIAGEN kit handbook or user manual QIAGEN kit handbooks and user manuals are available at www giagen com
4. and is ready for immediate DNA purification DNA can be purified from the rehydrated blood using a variety of different QIAGEN kits for manual or automated DNA purification The QlAamp DNA Blood Mini Kit and QlAamp DNA Mini Kit provide easy manual protocols Both protocols are fully automatable on the QlAcube Automation on EZ1 workstations provides purification of DNA from 1 6 samples per run using EZ1 DNA Blood Kits For higher throughputs the QGlAsymphony SP can process 1 96 samples per run using the QlAsymphony DNA Mini Kit 8 QlAsafe DNA Blood Handbook 09 2009 Equipment and Reagents to Be Supplied by User When working with chemicals always wear a suitable lab coat disposable gloves and protective goggles For more information consult the appropriate material safety data sheets MSDSs available from the product supplier For storage of samples in QlAsafe DNA Blood Tubes or Plates Wi Pipets and pipet tips pipet tips with aerosol barriers for preventing cross contamination are recommended Recommended Laminar flow hood or a ventilated incubator set to 40 C Shaker with an adapter for a microplate Heat sealer Optional Dry storage cabinet with relative humidity below 40 e g dry storage cabinets from Biomatrica see www biomatrica com drystoragecabinets php For purification of DNA from QlAsafe DNA Blood Tubes or Plates Wi Pipets and pipet tips pipet tips with aerosol barriers for preventing cross contam
5. or can be requested from QIAGEN Technical Services or your local distributor SSSS ee SSSSS gt gt gt gt gt _ ooooy ll E EE EEee 18 QlAsafe DNA Blood Handbook 09 2009 Trademarks QIAGEN QlAamp QlAcube GlAsafe QlAsymphony EZ1 QIAGEN Group SampleMatrix Biomatrica Inc Limited License Agreement Use of this product signifies the agreement of any purchaser or user of QlAsafe DNA Blood Tubes or QlAsafe DNA Blood 48 Well Plates to the following terms 1 QlAsafe DNA Blood Tubes or QlAsafe DNA DNA Blood 48 Well Plates may be used solely in accordance with the QlAsafe DNA Blood Handbook and for use with components contained in the Kit only QIAGEN grants no license under any of its intellectual property to use or incorporate the enclosed components of this Kit with any components not included within this Kit except as described in the QlAsafe DNA Blood Handbook and additional protocols available at www giagen com Other than expressly stated licenses QIAGEN makes no warranty that this Kit and or its use s do not infringe the rights of third parties This Kit and its components are licensed for one time use and may not be reused refurbished or resold QIAGEN specifically disclaims any other licenses expressed or implied other than those expressly stated C exp i09 NS The purchaser and user of the Kit agree not to take or permit anyone else to take any steps that could lead to or facilitate any acts pro
6. September 2009 QlAsafe DNA Blood Handbook For room temperature storage and archiving of whole blood for subsequent DNA purification This product contains DNA SampleMatrix technology developed by Biomatrica Inc QIAGEN Sample amp Assay Technologies QIAGEN Sample and Assay Technologies QIAGEN is the leading provider of innovative sample and assay technologies enabling the isolation and detection of contents of any biological sample Our advanced high quality products and services ensure success from sample to result QIAGEN sets standards in Purification of DNA RNA and proteins Nucleic acid and protein assays microRNA research and RNAi Automation of sample and assay technologies Our mission is to enable you to achieve outstanding success and breakthroughs For more information visit www giagen com Contents Kit Contents Storage Product Use Limitations Product Warranty and Satisfaction Guarantee Technical Assistance Safety Information Quality Control Introduction Principle and procedure ON No BOW UH UU BB BS Equipment and Reagents to Be Supplied by User Protocols B Sample Drying and Storage 10 B Sample Recovery 12 Troubleshooting Guide 14 Appendix Determination of Concentration Yield and Purity 16 References 16 Ordering Information 17 QlAsafe DNA Blood Handbook 09 2009 3 Kit Contents 48 Well QlAsafe DNA Blood Tubes 50 Plates 10 Catalog no
7. atisfactorily due to any reason other than misuse QIAGEN will replace it free of charge or refund the purchase price We reserve the right to change alter or modify any product to enhance its performance and design If a QIAGEN product does not meet your expectations simply call your local Technical Service Department or distributor We will credit your account or exchange the product as you wish Separate conditions apply to QIAGEN scientific instruments service products and to products shipped on dry ice Please inquire for more information A copy of QIAGEN terms and conditions can be obtained on request and is also provided on the back of our invoices If you have questions about product specifications or performance please call QIAGEN Technical Services or your local distributor see back cover or visit www giagen com QlAsafe DNA Blood Handbook 09 2009 5 Technical Assistance At QIAGEN we pride ourselves on the quality and availability of our technical support Our Technical Service Departments are staffed by experienced scientists with extensive practical and theoretical expertise in sample and assay technologies and the use of QIAGEN products If you have any questions or experience any difficulties regarding QlAsafe DNA Blood Tubes and 48 Well Plates or QIAGEN products in general please do not hesitate to contact us QIAGEN customers are a major source of information regarding advanced or specialized uses of our product
8. bes 48 well plates 50 3 100 5 4 200 6 Note Drying times in Table 1 and Table 2 are only estimations Depending on the relative humidity drying times may vary significantly Drying should occur at 15 40 C with relative humidity below 50 Please be aware that drying in a nonventilated incubator leads to elevated relative humidity 4 Cover the samples after drying After complete sample drying plates should be covered with an AirPore Tape Sheet provided QlIAsafe DNA Blood Tubes can be closed using the caps supplied with the tubes 5 Place the samples including a desiccant in a heat sealed moisture barrier bag Remove a desiccant packet from the protective bag and place it together with the sample in the moisture barrier bag Make sure that the color indicator beads of the desiccants are dark blue pink beads indicate exposure to moisture in this case the desiccants should not be used Moisture exposed desiccant packets can be recovered by placing them in a 110 C oven until they turn dark blue For extended storage times 76 months moisture barrier bags should be heat sealed Alternatively tubes or plates may be stored in a dry storage cabinet with relative humidity below 4096 see Equipment and Reagents to Be Supplied by User page 9 Relative humidity above 5096 for extended periods of time will reduce product performance and sample protection QlAsafe DNA Blood Handbook 09 2009 11 Protocol Sample Recovery
9. blood cell count of the original sample e Inappropriate DNA Check the protocol for DNA purification We purification method recommend using a method listed in Table 4 in Protocol Sample Recovery page 13 Desiccant bead color is pink Desiccant exposed to Use a fresh desiccant packet or recover used humidity desiccant by placing it in a 110 C oven until the indicator beads turn dark blue Blood is not completely dry after overnight drying a Too many plates dried Avoid drying too many samples at the same simultaneously time since this leads to elevated humidity which results in prolonged drying times Extend the drying time until all samples are completely dry 14 QlAsafe DNA Blood Handbook 09 2009 Comments and suggestions b No ventilation Thorough ventilation is essential for complete drying of the samples Dry the samples either in a ventilated incubator set to 40 C or in a laminar flow hood at room temperature Extend the drying time until all samples are completely dry c Humidity too high Drying of the samples should occur at relative humidity below 50 If the relative humidity is higher we recommend drying samples in a ventilated incubator set to 40 C Blood does not rehydrate within 45 min a Inappropriate sample Storage at elevated temperatures gt 30 C as storage well as elevated relative humidity gt 40 may lead to prolonged sample rehydration as well as loss of sample integrity To rehydrate diffi
10. cult samples extend the rehydration time b Difficult samples Some samples stored for extremely long periods of time may be more difficult to rehydrate Extend the rehydration time to 1 2 h and if using QlAsafe DNA Blood 48 Well Plates add an additional 300 pl of rehydration solution e SSE QlAsafe DNA Blood Handbook 09 2009 15 Appendix Determination of Concentration Yield and Purity DNA yield is determined from the concentration of DNA in the eluate measured by absorbance at 260 nm Absorbance readings at 260 nm should fall between 0 1 and 1 0 to be accurate Sample dilution should be adjusted accordingly for example an eluate containing 25 50 ng DNA ul Ago 0 5 1 0 should not be diluted with more than 4 volumes of buffer Use elution buffer or water as appropriate to dilute samples and to calibrate the spectrophotometer Measure the absorbance at 260 and 280 nm or scan absorbance from 220 to 320 nm a scan will show if there are other factors affecting absorbance at 260 nm Both DNA and RNA are measured with a spectrophotometer to measure only DNA a fluorimeter must be used Purity is determined by calculating the ratio of absorbance at 260 nm to absorbance at 280 nm Pure DNA has an Aj60 Azg9 ratio of 1 7 1 9 References QIAGEN maintains a large up to date online database of scientific publications utilizing QIAGEN products Comprehensive search options allow you to find the articles you need ei
11. es DNA at room temperature The synthetic chemical matrix formulation is based upon the natural principles of anhydrobiosis Anhydrobiosis meaning life without water is a biological mechanism employed by some multicellular organisms that enables their survival in a dry state for periods over 100 years Accelerated aging studies A temperature increase of 10 C corresponds to a twofold increase in shelf life For example DNA stored at 40 C for 2 years corresponds to an estimated storage time of 8 years at 20 C t Hemmerich K J 1998 General Aging Theory and Simplified Protocol for Accelerated Aging of Medical Devices Medical Device Link www devicelink com mpb archive 98 07 002 html Crowe J H Carpenter J F and Crowe L M 1998 The role of vitrification in anhydrobiosis Annu Rev Physiol 60 73 QlAsafe DNA Blood Handbook 09 2009 7 The QlAsafe DNA Blood matrix forms a protective seal around DNA as it dries effectively shrink wrapping the sample in a protective coating Drying can occur at room temperature Stored dry at ambient temperatures the protected blood can be safely archived for extended time periods Blood DNA stored with the QlAsafe DNA Blood matrix is stable for years at room temperature Sample integrity is optimized when the plates or tubes are stored in the supplied moisture barrier bags with desiccant Blood can be recovered from the QlAsafe DNA Blood matrix through a simple rehydration protocol
12. hibited above QIAGEN may enforce the prohibitions of this Limited License Agreement in any Court and shall recover all its investigative and Court costs including attorney fees in any action to enforce this Limited License Agreement or any of its intellectual property rights relating to the Kit and or its components For updated license terms see www giagen com 2009 QIAGEN all rights reserved ss www qiagen com Australia Orders 03 9840 9800 Fax 03 9840 9888 Technical 1 800 243 066 Austria Orders 0800 28 10 10 Fax 0800 28 10 19 Technical 0800 28 10 11 Belgium Orders 0800 79612 Fax 0800 79611 Technical 0800 79556 Brazil Orders 0800 557779 Fax 55 11 5079 4001 Technical 0800 557779 Canada Orders 800 572 9613 Fax 800 713 5951 Technical 800 DNA PREP 800 362 7737 China Orders 021 3865 3865 Fax 021 3865 3965 Technical 800 988 0325 Denmark Orders 80 885945 Fax 80 885944 Technical 80 885942 Finland Orders 0800 914416 Fax 0800 914415 Technical 0800 914413 France Orders 01 60 920 926 Fax 01 60 920 925 Technical 01 60 920 930 Offers 01 60 920 928 Germany Orders 02103 29 12000 Fax 02103 29 22000 Technical 02103 29 12400 Hong Kong Orders 800 933 965 Fax 800 930 439 Technical 800 930 425 Ireland Orders 1800 555 049 Fax 1800 555 048 Technical 1800 555 061 Italy Orders 02 33430 420 Fax 02 33430 426 Technical 800 787980 Japan Telephone 03 6890 7300 Fax 03 5547 0818 Tec
13. hnical 03 6890 7300 Korea South Orders 1544 7145 Fax 1544 7146 Technical 1544 7145 Luxembourg Orders 8002 2076 Fax 8002 2073 Technical 8002 2067 Mexico Orders 01 800 7742 639 Fax 01 800 1122 330 Technical 01 800 7742 639 The Netherlands Orders 0800 0229592 Fax 0800 0229593 Technical 0800 0229602 Norway Orders 800 18859 Fax 800 18817 Technical 800 18712 Singapore Orders 65 67775366 Fax 65 67785177 Technical 65 67775366 Spain Orders 91 630 7050 Fax 91 630 5145 Technical 91 630 7050 Sweden Orders 020 790282 Fax 020 790582 Technical 020 798328 Switzerland Orders 055 254 22 11 Fax 055 254 22 13 Technical 055 254 22 12 e e EA e e UK Orders 01293 422 911 Fax 01293 422 922 Technical 01293 422 999 oee ee USA Orders 800 426 8157 Fax 800 718 2056 Technical 800 DNA PREP 800 362 7737 QIAGEN opie Sample amp Assay Technologies
14. ilizing DNA in blood at room temperature saves on refrigeration costs and enables easy transportation and storage QlAsafe technology was designed by combining extremophile biology and synthetic chemistry to create a novel dissolvable matrix optimally formulated for long term ambient temperature storage and shipping of whole blood Rehydrated blood samples are ready for immediate DNA purification using standard DNA purification techniques such as the QlAamp DNA Blood Mini Kit The purified DNA is suitable for any downstream application including PCR quantitative PCR and sequencing Blood samples stored dry in the QlAsafe DNA Blood matrix are ready for convenient shipping at ambient temperatures even over extended transit times After applying and subsequently drying blood samples on the matrix in QlAsafe DNA Blood Tubes or Plates DNA is stabilized in situ for years at room temperature 15 25 C as demonstrated by accelerated aging studies QlAsafe DNA Blood Tubes or Plates can be easily transported archived or immediately processed Principle and procedure The QlAsafe DNA Blood procedure consists of 3 parts sample application sample storage and nucleic acid purification Blood samples are directly applied to the QlAsafe DNA Blood Tubes or Plates After storage DNA can be purified using standard DNA purification technologies for whole blood The QlAsafe DNA Blood matrix is a mixture of dissolvable compounds that stabiliz
15. ination are recommended E QlAsafe DNA Blood Rehydration Set E Thermal shaker with an adapter for a microplate E QlAamp DNA Blood Mini Kit cat no 51104 QlAamp DNA Mini Kit cat no 51304 EZ1 DNA Blood 200 ul Kit cat no 951034 or QlAsymphony DNA Mini Kit cat no 931236 This is not a complete list of suppliers and does not include many important vendors of biological supplies eEeEEeaeS aeaeeeeeeeeeeeeeeee QlAsafe DNA Blood Handbook 09 2009 9 Protocol Sample Drying and Storage The QlAsafe DNA Blood matrix is formulated so that upon application of liquid samples the matrix dissolves and forms a protective coating The sample must then be completely dried for maximum protection and stability during storage at ambient temperatures Important points before starting E For optimal protection in the QlAsafe DNA Blood matrix do not apply more than a total of 200 ul blood per well or tube Samples should be dried in a laminar flow hood or in a ventilated incubator set to 40 C At an elevated relative humidity 2 5096 drying time may be significantly prolonged Blood samples applied into wells or tubes containing the QlAsafe DNA Blood matrix must be dried completely for optimal storage at room temperature QW Fresh or frozen whole blood collected with EDTA sodium citrate or heparin as anticoagulants can be used E Always wear gloves when handling QlAsafe DNA Blood Tubes or Plates to avoid contamination Proced
16. l be returned to storage make sure that the color indicator beads of the desiccant are blue If the beads are pink either use a fresh desiccant or recover the desiccant by placing it in a 110 C oven until the beads turn dark blue If the plate will be stored for longer than 6 weeks heat seal the moisture barrier bag again QlAsafe DNA Blood Handbook 09 2009 13 Troubleshooting Guide This troubleshooting guide may be helpful in solving any problems that may arise For more information see also the Frequently Asked Questions page at our Technical Support Center www qgiagen com FAQ FAQList aspx The scientists in QIAGEN Technical Services are always happy to answer any questions you may have about either the information and protocols in this handbook or sample and assay technologies for contact information see back cover or visit www giagen com Comments and suggestions Eluate contains degraded DNA or little or no DNA a Samples not properly Samples must be completely dried before dried storage See Tables 1 and 2 for guidelines in Protocol Sample Drying and Storage page 11 b Moisture barrier bags Moisture barrier bags must be heat sealed to not heat sealed prevent moisture reaching the sample c Sample stored in a Use desiccant packets and heat sealed moisture humid environment barrier bags to maintain samples at low humidity d Low amount of DNA in DNA yield depends on the blood volume and the initial sample white
17. s This information is helpful to other scientists as well as to the researchers at QIAGEN We therefore encourage you to contact us if you have any suggestions about product performance or new applications and techniques For technical assistance and more information please see our Technical Support Center at www giagen com Support or call one of the QIAGEN Technical Service Departments or local distributors see back cover or visit www qiagen com Safety Information When working with chemicals always wear a suitable lab coat disposable gloves and protective goggles For more information please consult the appropriate material safety data sheets MSDSs These are available online in convenient and compact PDF format at www qgiagen com Support MSDS aspx where you can find view and print the MSDS for each QIAGEN kit and kit component 24 hour emergency information Emergency medical information in English French and German can be obtained 24 hours a day from Poison Information Center Mainz Germany Tel 49 6131 19240 Quality Control In accordance with QIAGEN s ISO certified Quality Management System each lot of QlAsafe DNA Blood Tubes and 48 Well Plates is tested against predetermined specifications to ensure consistent product quality 6 QlAsafe DNA Blood Handbook 09 2009 Introduction QlAsafe DNA Blood Tubes and 48 Well Plates provide innovative technology for biological sample storage at room temperature Stab
18. tes at relative humidity above 40 for extended periods of time will reduce product performance and sample protection Lyophilized QIAGEN Protease can be stored at room temperature 15 25 C for up to 12 months without any decrease in performance For storage longer than 12 months or if ambient temperatures constantly exceed 25 C QIAGEN Protease should be stored dry at 2 8 C Reconstituted QIAGEN Protease is stable for 2 months when stored at 2 8 C Keeping the QIAGEN Protease stock solution at room temperature for prolonged periods of time should be avoided Storage at 20 C will prolong its life but repeated freezing and thawing should be avoided Dividing the solution into aliquots and storage at 20 C is recommended Product Use Limitations QlAsafe DNA Blood Tubes and 48 Well Plates are intended for molecular biology applications These products are neither intended for the diagnosis prevention or treatment of a disease nor have they been validated for such use either alone or in combination with other products Therefore the performance characteristics of the products for clinical use i e diagnostic prognostic therapeutic or blood banking are unknown Product Warranty and Satisfaction Guarantee QIAGEN guarantees the performance of all products in the manner described in our product literature The purchaser must determine the suitability of the product for its particular use Should any product fail to perform s
19. ther by a simple keyword search or by specifying the application research area title etc For a complete list of references visit the QIAGEN Reference Database online at www qiagen com RefDB search asp or contact QIAGEN Technical Services or your local distributor ll e a E 16 QlAsafe DNA Blood Handbook 09 2009 Ordering Information Product Contents Cat no QlAsafe DNA Blood For 50 samples QlAsafe DNA Blood 159134 Tubes 50 Tubes QlAsafe DNA Blood Tube Carrier Plate QlAsafe Desiccant and QlAsafe Moisture Barrier Bags QlAsafe DNA Blood For 10 x 48 samples QlAsafe DNA 159142 48 Well Plates 10 Blood 48 Well Plates AirPore Tape Sheets QlAsafe Desiccant and QlAsafe Moisture Barrier Bags QlAsafe DNA Blood For 100 samples QIAGEN Protease 159155 Rehydration Set 100 Protease Resuspension Buffer and RNase Free Water Accessories AirPore Tape Sheets 50 AirPore Tape Sheets 19571 50 Desiccant 1000 1000 Desiccant Packets 1 g each 159320 Related products for DNA purification QlAamp DNA Blood Mini Kit for purification of up to 12 ug genomic mitochondrial or viral DNA from blood and related body fluids QlAamp DNA Blood For 50 DNA minipreps 50 QlAamp 51104 Mini Kit 50 Mini Spin Columns QIAGEN Protease Reagents Buffers Collection Tubes 2 ml QlAamp DNA Blood For 250 DNA minipreps 250 QlAamp 51106 Mini Kit 250 Mini Spin Columns QIAGEN Protease Reagents Buffers Collection Tubes 2 ml QlAamp DNA
20. thermal shaker and incubate at 56 C with continuous shaking at 600 rpm until the blood is rehydrated Rehydration time is dependent on the original blood volume and the storage time and may vary from 2 to 45 min until the sample returns to liquid form WAAAAAA AAAAAAAAAAAAAAAAAAAAA e f 12 QlAsafe DNA Blood Handbook 09 2009 To avoid cross contamination of samples do not shake at higher speeds 3 Follow the kit protocol for manual or automated purification of DNA from blood Table 4 lists QIAGEN kits that can be used for the purification protocol The rehydrated blood should be treated as fresh blood DNA can be purified without any further dilution or pretreatment of the rehydrated blood Table 4 QIAGEN kits for DNA purification Kit Automation Protocol Cat no QlAamp DNA Manual or automated Blood and body 51104 Blood Mini Kit on the QlAcube fluid QlAamp DNA Manual or automated Blood and body 51304 Mini Kit on the QlAcube fluid EZ1 DNA Blood EZ1 workstations DNA Blood 200 951034 200 ul Kit QlAsymphony QlAsymphony SP Blood 200 FIX 931236 DNA Mini Kit This table shows only a selection of QIAGEN kits that can be used with QlAsafe DNA Blood Tubes and Plates To find out more about other QIAGEN genomic DNA purification kits such as kits for high throughput 96 well purification please visit www qiagen com DNA Note If only some wells of a QlAsafe DNA Blood 48 Well Plate were rehydrated and if the plate wil
21. ure 1 Remove the seal or cap and gently apply 50 200 pl of whole blood into the center of the vessel containing the QlAsafe DNA Blood matrix The QlAsafe DNA Blood matrix is supplied as a coating on the bottom of each well or tube Note If storing 50 100 ul blood in a well of a QlAsafe DNA Blood 48 Well Plate first add 50 ul nuclease free water and then the blood sample to the well 2 Mix the sample by placing the QlAsafe DNA Blood 48 Well Plate or Tube placed in the provided tube carrier plate on a microplate shaker and shake for 15 min at 600 rpm To avoid cross contamination of samples do not shake at higher speeds 3 Dry the uncovered sample completely under a laminar flow at room temperature 15 25 C or by placing in a ventilated incubator or oven set to 40 C Drying times are dependent on the blood volume and the drying conditions Approximate drying times are given in Tables 1 and 2 on page 11 However to ensure complete drying we recommend drying the samples overnight i e gt 16 h using a laminar flow hood at room temperature or a ventilated incubator set to 40 C EEO el 10 QlAsafe DNA Blood Handbook 09 2009 Table 1 Approximate drying times in a laminar flow hood Approximate drying time h Sample volume pl Tubes 48 well plates 50 4 2 100 6 7 200 8 14 Table 2 Approximate drying times in a ventilated incubator at 40 C Approximate drying time h Sample volume pl Tu
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