User manual for version 4
Contents
1. Rate G C min Sample controlled mode You can enter a start temperature and a heating rate Then press the Goto button to send the hot stage to the starting temp Once the temp is stabilised press the Start button to begin the program You can alter the heating rate by entering a new value or increase by a factor of 5 or 10 up to a maximum rate of 20K min You can also pause the program and alternate between heating and cooling In remote control mode you must manually start and stop the video capture Due to temperature lags in the system the set point temperature may be in advance of the actual temperature when using faster heating rates above 6K min or when switching rapidly between heating and cooling Sample Controlled Hot Stage Microscopy option Sample controlled mode if Use zero rate 4384 Signal 200 Lo threshold 1000 Hi threshold 5 Timeout 5 Smoothing This uses a feedback loop based on the changing light intensity to alter the heating rate When the intensity is changing the heating rate is reduced and when the light intensity is constant the rate is increased The current changing intensity value is displayed as the signal The user can set a number of parameters in this mode as follows 1 Lo threshold this sets the value for the change in light intensity below which the system will increase the heating rate 2 Hi threshold this sets the value for the change in light intensity above which th2. the FP90 82 and FP90 84 Hot Stage systems from Mettler Toledo You can connect the FP90 82 84 to either the Com1 or Com2 ports If you connect it to Com2 then select Com2 from the Controller menu If you are not connecting a hot stage then select no controller from the controller menu You can also configure the system to control the video capture from the FP90 Check the autostart stop menu to activate FP90 control Note that this is not suitable for use with linked methods as the video file will be stopped at the end of the first method For these applications you should manually stop the video or pre set the length of the video file capture menu video file options When the hot stage is connected the temperature can be overlayed into the image This is controlled by the temperature icon on the toolbar You can select C or Kelvin for the temperature units default is C from the settings menu Settings Help 103 Sample name uu T Temp units ie ie 0 colour K Remote control function Controller Video Capture Ea Autostart Ctrl a Remote control Ctri r This allows you to drive the FP90 from the software To activate remote control mode select Remote Control from the controller menu press Ctrl r and the controller window will open To move the remote controller window click on the gripper at the bottom and drag the window Remote Controller 4 FPSO control Temp 142 C Limit 150 c
3. to open a new dialog box Capture Settings Help Select Compression CODEC Image save mode d Curent CODEC MS A k g Video File options E i Select video compressor T elect new CODEC CaptureR ate d MS MPEG4 Y2 Log Daka Ctrl d Hi light the required CODEC from the list and press OK to accept and save or press cancel to retain the current setting If you chose Chose and configure CODEC then you will be prompted for a CODEC as part of the video file setup procedure Note that the chosen CODEC is save and reselected when you next start Studio Capture Once the CODEC Is defined you can click the film icon on the toolbar to open a file dialog where you can enter a filename and directory to save the video file If an overlay title is set then the filename will initialise to this name gt If you previously close to Select from all installed CODEC s you are required to select the CODEC from the drop down list You can also select advanced CODEC options if available Select Video Compressor Sc Microsoft MPEG 4 Video Code Cancel Configure About Data Rate After you select the CODEC the system is ready to start recording but is paused You can start recording by pressing the OK button on the window that appears on top of the video image Recording is paused Press OF to start recording If you have selected to use the FP90 to control the video capture autostart then recording will s
4. Studio Capture m Video microscopy software Introduction Studio Capture is a video and still image capturing software with special overlay facilities Images and video s can be annotated with title scalebar date time and when connected temperature from a microscope hot stage Studio Capture Is designed for time lapse video capture i e playback frame rate greater than capture frame rate making it ideal for slow processes Still images can also be captured allowing your video camera to be used as a digital still camera Hardware requirements Studio Capture is designed to operate with standard frame grabber cards using Windows driver model wdm direct show drivers Suitable cards include those from Osprey Hauppauge and Pinnacle systems In addition you need a video camera with suitable connection to your frame grabber card Digital cameras using USB2 and IEEE firewire interface can also be used provided they have wdm direct show drivers Connecting a video source a When you start Studio Capture you will need to connect to your video source Click on the select video device button on the toolbar The installed devices will be listed and you should select the device you want to connect to The video will be displayed in the main program window You can resize the window by dragging the corners or using the window controls minimise restore and maximise Please note that resizing the window does not change
5. Time display You can change the colour of the overlayed text and lines From the settings menu select Colour to open a colour selection dialog box Select the colour to your liking and press OK to accept or Cancel Setting options for image file saving mode Capture Settings Help Image save mode e Single File Video File options SEQUENCE You can save images in bitmap and jpeg format Also you can choose to save single images or a series of images The Capture image save mode menu allows you to select single file or sequence modes Saving bitmap files To save an image as a bitmap bmp file click on the save bmp button on the toolbar A file dialog box opens where you can select a filename and directory to save the image Saving j peg files im i To save an image as a compressed j peg jpg file click the save jpeg button on the toolbar A file dialog box opens where you can select a filename and directory to save the image Copy to Clipboard You can copy an image directly to the system clipboard for transfer into other applications Click the copy button on the toolbar Video file length The end of the video file can be controlled by the user stop button triggered by the FP90 controller or programmed to auto stop after a certain time To have the video start and stop to be triggered by the FP90 controller make sure that the autostart stop menu item is checked in the controller menu Controller Video Capt
6. alogue video camera s The video display is now scaleable allowing the program window to be resized A digitally zoomed area of the video can now be displayed The position of the text overlay can be adjusted The video start and stop can now be triggered by the FP90 hot stage controller The last used frame capture rate is saved and restored at the start of each session The last used FP90 Connection setting and autostart option are saved and restored at the start of each session The data logging setting is saved and restored at the start of each session The last used video compression CODEC is saved and restored at the start of each session The time display can be toggled between real time and relative time since start of video file capture Still images can be captured individually or automatically in a sequence The line width of overlay items such as ruler and scalebar can be selected Settings menu line width Camera settings not available under the video settings menu can now be accessed by using the IEEE set camera w b menu
7. e system will decrease the heating rate 3 timeout this sets the delay time in seconds following a change in rate before another change will take place 4 smoothing this sets the number of values to be averaged in the derivative calculation 5 SCHSM active checkbox activates sample controlled mode 6 Use zero rate checkbox determines if heating rate can go to 0 or 0 1K min as minimum Note that in sample controlled mode the variable heating rates are pre set to this sequence 0 0 1 0 2 0 5 1 2 3 and 6 C min You should start a heating or cooling ramp before activating the sample control mode and set the initial rate to 6 C min During the measurement you can adjust the Hi and Lo threshold Smoothing and timeout values to suit the sample you are analysing In remote control mode you must manually start and stop the video capture Due to temperature lags in the system the setpoint temperature may be in advance of the actual temperature when using faster heating rates above 6K min or when switching rapidly between heating and cooling V Temp function Studio Capture can also be used with other temperature controlling devices such as DSC systems and hot stages from other manufacturers In these cases the actual temperature is not sent to Studio Capture but the V temp function provides a convenient way of displaying the assumed temperature onto the video image This adds greatly to the usefulness of these other thermo optical m
8. easurements Setting up the V temp display In order to use V temp the no controller option must be selected from the controller menu You may need to disconnect and reconnect the video device in order to do this Controller Video Capture ol Autostart Ctrl a Remote control Ctri r Click on the V temp menu and select the V temp settings option Y Temp Help T Temp Settings The V temp control window opens V Temp Settings Use Temp Segment type Dynamic Isothermal Add Delete Clear All Segment settings JF Start temp segment 1 2p0 End Temp 10 Rate Synchro start z Use synchronisation signal from port Lok Com 1 Com 2 C Com 3 J Com 4 Cancel In the v Temp settings window you can set a sequence of segments to match the temperature program of your test instrument Up to 10 segments can be defined Each segment can be dynamic heat or cool or isothermal constant temperature For each segment define the type with the dynamic or isothermal button and then enter the appropriate values Click the Add button to add a new segment or the Delete button to delete the last segment Click the Clear button to clear all the segment data It may also be possible to synchronise the start of the temperature program and the video capture For this you need a special cable details are available from your vendor and your heating device must also be able to trigger a signal switch at t
9. file contains 4 columns of data Column 1 is the time relative to the start of the test column 2 is the temperature column 3 is the average image intensity brightness and column 4 is the optional output of the FP90 controller The value of this 4th column depends upon which module is connected With the FP82 the data is the output from the photomonitor With the FP84 the data is the DTA signal Note that if the maxrate is set for the video frame capture rate then the time index will increase by 1 every time a frame is saved This is because the actual time interval between frames will vary from system to system For all other frame capture rates the time interval between data points will be correct Using the data file At the end of the measurement the data file can be opened in graph drawing software such as Microsoft Excel double clicking on the file will open it in Excel thanks to the xls file extension Other graphing software can also be used since the data format in the file is tab separated Capture frame rate Capture Settings Help Image save mode hm SH Video file options Ei Select video compressor CaptureRate Max Rate Log Data Ctrl d e 1 Fps spf 5 spf 10 spf 30 spf You can select the video capture frame rate from the Capture menu The active rate will be ticked and also shown in the status bar at the bottom of the main window To select a different rate hi light the new rate from the list The ra
10. he start of the test The DSC823e and DSC1 instruments from Mettler Toledo have such functionality when fitted with the peripheral option board You must also select the commport to which the cable is attached on your PC and make sure the use synchronisation check box Is checked When you have defined your segment s make sure the Use V temp check box is ticked and click the OK button to return to the main window Now set up the video file If you are using synchro start then the video will begin saving automatically when the temperature program starts Otherwise you must press the OK button on the paused screen when the temperature program starts If you have set the correct program conditions the display will match the actual temperature of your instrument Video recording stops automatically at the end of the measurement Registration Studio Capture can be used for evaluation purposes During this time a watermark will be displayed on all images and video frames Once you have purchased Studio Capture you will be sent a user name and registration key To enter these Press the Help menu and license sub menu to open the registration window Registration Hame Reaistration Rey UE Cancel Enter your username and registration key and press the OK button This will remove the watermark Resizing the window The program window can be resized by dragging the control handles or using the min max restore bu
11. ll video formats and with all CODEC s You Should experiment with different settings to see which are best on your system Settings Help Sample name S fit R Temp units Colour Font Line width ETFI Refresh rate Slow Cbrl s t Time display e Normal Ctrlt n Fast Ctrl F Auto connecting video device You can configure Studio Capture to automatically connect to your video camera on start up Make sure the video device autoconnect menu Is checked Video Capture Settings Help Device _ gt Settings Disconnect Size Autoconnect Adjusting other video settings Video Capture Setting Device d H 4 corner overlay Compact overlay Set IEEE WB Some firewire cameras may require you to use their own software to set some camera parameters such as white balance To do this click on the video set IEEE camera W B menu The first time you do this you will be prompted to locate the application The camera software will start and you can set the white balance When you close the camera application you will return to Studio Capture and you can reconnect the camera using the camera toolbar button or the video device connect menu Selecting overlays ID Tt mt The video and image files can include overlayed information Options are Sample ID A sample ID can be entered and displayed Click on the ID toolbar button to toggle this overlay and click on the settings sample name men
12. ng systems and incorporates an updated video capture toolkit Studio Capture version 4 now allows up to 10 segments to be defined and used in V Temp mode If used with a METTLER TOLEDO DSC system the video start can be synchronised using a special connection cable If you are upgrading from an earlier version you will need to purchase a software update license from your local vendor New features introduced in version 3 1 Studio Capture version 3 1 introduces a new method of controlling the heating program Sample Controlled Hot Stage Microscopy allows the heating rate to change during the measurement slowing down as the sample changes and speeding up when the sample is Stable This can be particularly useful for studying polymorphic transitions and crystallisations V temp is a new function that allows temperature to be displayed onto the video file even if no direct temperature signal is available This is useful for working with other thermal instruments such as DSC s New features introduced in version 3 Studio Capture version 3 incorporates a simple remote control function for the FP90 allowing you to interactively drive the hot stage from the software Studio Capture version 3 now supports the latest Windows XP style user interface New features introduced in version 2 Studio Capture version 2 incorporates a new video capture toolkit which supports the latest EEE firewire digital cameras and wdm driver video capture devices for an
13. s to the common controls in Studio Capture From left to right they are Video file setup Video file start Video file stop bmp file save jpg file save Copy Video device Video format Video inputs Video settings Title overlay Temperature overlay Time overlay Scale bar Ruler Calibration Zoom Help initialise avi file start pause recording stop recording save bitmap image save j peg image copy image to clipboard select video input device set video size and colour depth select video input channel S VHS Composite set video contrast and brightness toggle title overlay toggle temperature overlay toggle time overlay toggle scalebar overlay toggle ruler overlay open microscope calibration menu show a digitally zoomed area of the video display help file Overlay Font Settings Help 13 Sample name T Temp units Ii colour A Font Line width gt Refresh rate F t Time display You can change the style and size of the text overlay From the settings menu select Font to open a font selection dialog box Select the font size and face to your liking Note that font style attributes such as bold italic and underline are not supported in this version of Studio Capture Press OK to confirm or cancel to ignore changes Overlay Colour Settings Help 13 Sample name T Temp units Di colour A Font Line width gt Refresh rate F t
14. tart as soon as the FP90 begins the measurement Recommended CODEC s are the Microsoft Mpeg4 V2 CODEC and the Microsoft Media 9 CODEC but you should experiment to find the most appropriate in terms of file size and image quality for your application Depending upon your system you may not be able to capture at 1 frame per second with large video images In this case you should reduce the capture rate or select a smaller video format If you send your video s to other people then they will need the same CODEC installed on their system in order to playback the video files Microscope Calibration Hi The overlay scalebar can be calibrated for up to 4 objective lenses Click the show calibration menu icon on the toolbar The scalebar will automatically be overlayed into the image if not already selected Microscope setup Objective calibration Mag scalebar Active nd s00 2 x 10 200 n 4 You can adjust the length of the overlayed scalebar using the slider control to be the same length as a stage reticule Up to 4 objective lenses can be installed and the magnification and scalebar length entered into the form These settings are automatically saved when you press the Set button To select the scalebar legend click the active radio button alongside the appropriate lens setting Hot stage controller Controller Video Capture Ea Autostart Ctrl a Remote control Ctri r Studio Capture is designed for use with
15. te chosen will affect the file size of the saved video and also the temperature resolution when used with a thermometer For temperature scanning work a capture rate of 1 frame per second fps is recommended along with a heating rate of 6 C min This gives 1 frame of video for every 0 1 C temperature change For long experiments capture rates of between 2 and 30 seconds per frame can be set to reduce file size If you chose max rate then the system will capture a frame as often as possible up to a maximum of 10 frames per second The actual rate is influenced by the video refresh rate Settings Help Sample name am iti cm p Temp units Colour Font Line width EFE Refresh rate Slow Cbrl s t Time display e Normal Ctrl n Fast Ctrl F If the refresh rate is set to slow then the max rate is 2 frames per second If the refresh rate is set to normal then the max rate is 5 frames per second If the refresh rate is set to fast then the max rate is 10 frames per second Depending upon the image size and your system performance the actual capture rate may be less that indicated above Setting up the video file In order to capture a video file you need to specify a filename and video CODEC compression used to reduce file sizes This can either be predefined for all video files or selected for each individual video file To predefine the CODEC click the Capture Select video compressor menu
16. the actual resolution of the video When you resize the window the effective zoom factor will be displayed in the status bar at the bottom of the screen To change the video size resolution press the set video size toolbar button The available video formats will be displayed along with the colour depth If possible select a 16 bit or 24 bit RGB colour depth If you have a frame grabber card that has more than one input then you will need to select the connected input Click on the show video input s toolbar button to display the input dialog box Select the input that your camera is connected to Changing video settings a You can change the brightness contrast and colour settings of the video signal Click the set brightness and contrast button on the toolbar Move the slider controls to adjust the settings to your liking or set auto adjust if available In addition you can select the video refresh rate which may be necessary on slower systems to improve performance Select the settings menu and then the refresh rate menu to show a selection box where you chose fast medium or slow The setting for refresh rate does not affect the video file frame capture rate except for when the capture rate is set to Max In this case the video capture rate also depends upon the refresh rate where refresh slow 2 fps refresh normal 5 fps and refresh fast 10 fps Depending upon the performance of your PC it may not be possible to save at 1Ofps in a
17. ttons in the top right hand corner You can also use the Video set 100 menu or press the F2 key to set the video display to 100 of the resolution When the window Is resized the video image Is also resized to fit into the window It should be noted that the video always retains the aspect ratio defined by the actual video resolution and this can result in the video image not completely filling the window or alternatively not being completely visible in the window Please carefully adjust the window size to give a complete view of the video It must also be noted that resizing the window does not affect the actual resolution of the video or image files saved Digitally zooming the image a A digitally zoomed area of the image can be displayed in a window Click the zoom toolbar button to open the window The window can display 1 of 5 areas of the image Set Area Edit Top Left Fi Top Right Fz Centre Fa Bottom Left F4 Bottom Right F5 El El el d 4 These can be selected from the Zoom window menu The options are top left corner top right corner centre bottom left corner and bottom right corner The zoom window can be resized by dragging the corners to adjust the zoom level To close the window press the zoom toolbar button You can also copy the zoomed image to the clipboard using the edit copy menu Changes in version 4 Studio Capture Version 4 has been optimised for use with Windows Vista as well as Windows XP operati
18. u to enter the name Temperature f the FP90 is connected then you can toggle the temperature overlay using the temperature toolbar button The units can be C or K and are selected from the settings Temp Unit s menu Date time The date and time overlay can be toggled using the toggle date time overlay toolbar button Scalebar The scalebar overlay can be toggled using the toggle scalebar overlay toolbar button Ruler Features in the image can be measured using the ruler function The ruler overlay is toggled using the toggle ruler overlay toolbar button To draw the ruler click the left and right mouse buttons whilst holding down the control Key A line is drawn between the points marked and the length displayed in the middle of the line Text background To enhance the visibility of the overlay text against changing images it can be drawn with a dark background This is switched on by default To toggle the background on off click on the video image whilst holding down the shift key Positioning overlay text The layout of the text overlay can be set in 1 of 2 positions Video Capture Settings Device d a 4 corner overlay Compact overlay Set IEEE WB 4 corner overlay places the text in the corners of the screen Compact overlay places the scalebar in the bottom right corner and all other text overlays in the bottom left corner of the video Toolbar commands OP ea PRRs BoD Tt Sma The toolbar provides rapid acces
19. ure s Autostart Ctrl a Remote control Ctri r The FP90 trigger should only be used for non linked methods If you are using linked methods for example a heat cool heat cycle then you should manually start and stop the video capture or use the timed auto stop function detailed below To program a timed auto stop useful for example if a long measurement Is being made select the Capture menu then video file options Video file options Video file setup i Video file length minutes set for continuous recording 15 Video playback rate frames second 0K Cancel Enter the video file length in minutes Max 999 This will calculate the number of frames to capture The number of frames to be captured will be displayed in the status bar during the capturing process Setting up the data file Capture Settings Help Image save mode d Video file options Ei Select video compressor CaptureRate d E Log Data Ctrl d In addition to saving a video file you can also chose to save digital data To active the data logging click the log data option under the capture menu if selected it is ticked If you want to deactivate data logging simply untick the data logging option The data file will be automatically created using the same name as your video file but using xls as the file extension The xls file will be saved in the same folder as your video file Contents of the data file The data
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