Shimadzu IRAffinity-1 - School of Chemistry and Biochemistry
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1. 20 s Ready R interns NUM GLP Since it is best to obtain a background spectrum just before obtaining your sample spectrum it is advisable to click No on the above message window The instrument has been preconfigured to initialize as soon as the IRSolution software starts Initialization may be verified on the Status window displayed on the bottom left corner of the IRSolution window Upon successful initialization the Status window should reflect this as shown below olution Measure _ Eile Edit Measurement AJM Graph Admin Manipulation Manipulation2 Search Quant Magro Print 3Dim Window Enyironment Help sROMPHAIA2AARGBSZE 2B ex ae BMeonse View Mariuaton seach Fut 54 5 Pirate BKG Sample Montor _ Hee View background Comment FTIR Measurement I Continuous jimit T Datafie C Program Files Shimadzu iRsokaion data NTINTOS 7 Auto increment More Fies Advanced x Daa instrument Measurement Mode Tranemetance w ri r Agodzaton Performing diagnostic power measureme Hopp Genzi Setting aperture open EEN Power diagnostic OK 116 1 179 9 89 ia E Bedin z 20 z 27 2012 Model IRAflin E Range om F Mn 550 EE hh Max 4000 Statue 1 r Performing diagnostic power measuiem Setting aperture open Power diagnostic OK 116 1 179 9 89 Ready 3 27 2012 Modet IRA2. evaporating the solvent 17 Gases level 5cm 10cm gas cell ppm level Long path gas cell Detection limit is O 1 1ppm with 10m optical path Micro trace samples Liquids uL level Micro cell Solids u level AIM 8800 Applicable to transmission reflection and ATR methods Source http www shimadzu com an spectro ftir accessory guide html 9 2 Table 4 Characteristic Infrared Absorptions of Common Functional Groups Formatted Heading 2 Indent First line 0 Line spacing single Bond Type of bond Specific type of bond Absorption Appearance peak con anyi methylene vinyl C C monosubstituted alkenes 18 C C C o wisusitedatkenes 8005840 em strong to medium me monosubstituted benzene srog o meta disub benzene 750 800 cm stron g 860 900 cm strong reee _ attest em alkynes eee Sl ean cm medium aldehydes Es cm medium 2820 em em seylie C C with C 0 C C both sp any 1640 medium 1680 cm aromatic C C weak to strong usually 3 or 4 terminal alkynes weak 2140 cm disubst alkynes very weak often indisinguishable 2260 cm aldehyde ketone saturated aliph cyclic 6 membered ae eel benzene ring 19 ejcicSmombeed sve gcietmnbaed fsa carboxylic acids derivates unsat aromatic carb acids 1680 1690 cm esters and lactones 1735 cm influenced by conjugation and ring size as teasom sem
3. of the bond which in turn depends on the atoms connected by the bond the type of bond involved and to a certain extent the environment around each bond Specifically the mid infrared range 4000 to 400 cm of frequencies is the most useful in organic chemistry Most functional groups have characteristic absorptions in this frequency range and these characteristic absorptions are used to identify what functional groups are present ina sample The characteristic absorptions of some common functional groups can be found in section 1 2 2 The Shimadzu IRAffinity 1 Spectrometer Pz feretuye Go pry amp p SERS AANA HR The Shimadzu IRAffinity 1 Spectrometer like most FTIR spectrometers makes use of a Michelson interferometer with a movable mirror A schematic diagram of this instrument is shown in Figure 1 The IRAffinity 1 uses a high energy long life ceramic light source 1 which sends light to a spherical mirror 2 The beam is then converged at the aperture 3 and is sent to the collimator 4 to make parallel beams that are sent to the interferometer The beam splitter 6 7 divides the light beam to the fixed mirror 8 and the moving mirror 9 When the distance between the beam splitter and the fixed mirror equals the distance between the moving mirror and the beam splitter the beams are in phase and interfere constructively When the moving mirror is displaced by 1 4 of the wavelength of Comment
4. BR1 I ve moved this to the appendix the beam from the source the two beams will be out of phase and will interfere destructively This optical path difference is measured very accurately via a He Ne laser In the IRAffinity 1 the beam splitter is made of a potassium bromide substrate on which germanium is evaporated This beam splitter is very easily damaged by moisture and as such it is protected with a moisture resistant coating Furthermore the instrument is equipped with a dehumidifier Nevertheless care must be taken to avoid moisture building up in the instrument These two beams are then reflected by two flat mirrors 10 and 11 and are converged at another mirror 13 The resulting parallel interfered beam then goes through the sample compartment Thereafter the beam goes through another converging mirror 14 and then to the detector 15 For this instrument the detector is a DLATGS deuterated L alanine triglycene sulfate pyroelectric detector which has a high sensitivity and does not require cooling Figure 1 As explained above the instrument is sensitive to humidity and therefore it has been equipped with a ROSAHL dehumidifier which keeps the instrument at low humidity However this unit must constantly be connected to a power supply to keep the dehumidifier running 3 How to Use the IRAffinity 1 FTIR system and the IRSolution software 3 1 Sampling Accessory Selection and Installation 3 1 1 MIRacl
5. Shimadzu IRAffinity 1 User Guide and Tutorial for Taking IR Measurements in the Reynolds Research Group 2 d Edition April 2012 Georgia Institute of Technology School of Chemistry amp Biochemistry School of Materials Science and Engineering Written by Natasha B Teran Table of Contents de WMEROUUGHONtactite Mitte ate tats tat ene Soe E ie eee eS ts Sia Sate eia 3 1 1 Basic Principles of Infrared SpectrOSCOpy esssessesessesssssessessesssssessesecntesneesneeseeseerseeessenseens 3 DED Socsden E E a decades alee E E E decd tsi E decease dade sauit cece 3 2 The Shimadzu IRAffinity 1 Spectrometer cesceessessseseessesseesnessesseesseesessseensecneeaseeseesesseeeseeeseens 3 2 1 Diagram of the sampling accessories and their specific applications s s s 3 How to Use the IRAffinity 1 FTIR system and the IRSolution Software esses 4 3 1 Sampling Accessory Selection and Installation sceseesessessssssesseeeseeeeeseentecneesneeseeseeeaes 4 3 1 1 MIRacle Single Reflection Horizontal ATR ACCESSOTY essessssssstsssteentestesneeseeseeees 4 3 1 2 30Spec 30 Degree Specular Reflectance ACCOSSOLY usessesessessssssstessteenteentesneeseesesaes 5 3 1 3 EasiDiff Diffuse Reflectance ACCESSOTY e eesssessesstesneeseeseseesessteestesssesteentesneesneeseeseeees 5 3 2 Starting up the FTIR System iiiter iets oisein saser rakoetan iaaiiai atan 5 3 2 1 Trouble shooting initialization rrr eesssesstessesseeseesesseeseeseeeseeeseesteenteenees
6. ance ssesessessssrsrsrrersrssneusvsnunenennnnnnnnnnnnunnununenennnnnnnnnnnnnnnnnunnnnunennnnrnnnnrnnras 15 5 2 gt Specular reflecta Cennin iinei a A N 15 Bids Diffus r fl ectan Ce isiiiimnnnnnn iasanen a aN ae 15 a SAEPE EEE E E EEE AE SE 15 6 Transferring data and preparation of spectra using other graphing software 15 7 Care and maintenance of the instruMent ce esesscessesssesseessessetsseessessetessieesessanessneesaess 15 7 1 Protection against DUMICILY oo eesseesseessessseeseesseesseseseeseeneesseesnessuesseesnetseesseeeaeesseenteenteaneesess 15 7 2 Protection against VIDTAtiONS ceecsescssscsscsssssesseeseesseersesessecsesesecssessecseessesesseetseseseeeaneeeeseneneecses 15 8 General Trouble sho otimgiscsccesscsscescesdscsiessdicsncesccsecsstcssecdsce scented seen sec sectivvesced sos aiaa aa aradi TSEAN 23 Shimadzu IRAffinity 1 User s Manual 1 Introduction 1 1 Basic Principles of Infrared Spectroscopy Infrared IR spectroscopy is one of the most common techniques used in organic chemistry for the identification of structural characteristics of various substances or mixtures thereof When an organic compound is subjected to electromagnetic radiation in the infrared frequency range 780 nm to 1000 um or 13 000 to 10 cm the absorbed resonant frequencies give the molecules enough energy to allow their bonds to stretch bend or rotate more actively The resonant frequency depends on the strength
7. ck OK The instrument will take a few minutes to perform the auto adjustment and a progress bar will be displayed idata NTANTOS V Auto increment Performing AutoAdjust x Cancel Check to make sure that all messages shown for a successful initialization are present see above 3 2 1 Trouble shooting initialization errors 3 3 Data acquisition 3 3 1 Setting the Scanning Parameters Normally the Scan Parameters need not be changed but if necessary it can be found while on the Measure tab on the bottom right corner of the screen It consists of five tabs namely Data Instrument More Files and Advanced Fic Edt Memuement AM Graph Admin Menipulstion Manipulstion2 Search Quant Mecro Brint 3Dim Window Enyironment Help SSS en Prins T BKG Sage Monta Si j Help FF View background Comment FTIAMesarement I Cor ruous mk fT Detefle C Progam Fles Shimads iReokton aaalNTINTOS FF Auto increment ri jotting aperture auto 11 37 24 AM 3 27 2012 Piezo Vokage Piezo Voltage 102 i 11 37 33 AM 3 27 2012 Piezo Vokags Pestoeming measure eing aperture open Power dagnostic Fated 246 386 20 ic faledi i AM 3727 2012 Fine Autordas jetting aperture 20 3 3 1 1 Data Tab The Data tab gives five parameters to set ooo More Files Advanced Data Instrument Measurement Mode Transmittance Apodization Happ Genzel v No of Scans 20 Resolution 4 0 Ran
8. commended 3 3 1 1 5 Range The Range set depends on the desired measurement and the detector Most commonly for this instrument a minimum of 400 and a maximum of 4000 cm are set 3 34 2 Instrument Tab The Instrument tab parameters are only changed when the instrument is coupled with an external IR microscope or a different sampling kit from the standard sample compartment and the M Racle sampling accessory Changing these parameters will be discussed under the section for Sampling Accessories The normal settings are Beam Tab 10 Comment E3 You mean quantification Detector Standard Mirror Speed 2 8 More Files Advanced Data Instrument Bem Detector standard z Miror Speed 3 3 1 3 More Tab Data Instrument More Fies Advanced m Normal Gain n Aperture auto gt m Monitor Mode Power 3 3 1 4 Files Tab The Files tab is used to set the saving parameters such as the destination directory and the file name To set click the Save As button To load saved parameters click the button 11 Data Instrument More Files Advanced Parameter 3 3 1 5 Advanced Tab Data Instrument More Files Advanced IFG Noise 1 IFG Similarity fi Write Aperture delay fo s IR Range mip Light source Infrared X 3 3 2 Obtaining a Background Spectrum Make sure that th
9. e 7 Care and maintenance of the instrument 7 1 Protection against humidity 7 2 Protection against vibrations 8 General Trouble shooting 9 Appendix lt Formatted Heading 1 Indent First line 0 9 1 Diagram ofthe sampling accessories and their specific applications Sample Analytical form and analytical Accessory condition Solids Rubbers Total reflectance method ATR 8000A etc MIRacle Dura sampler Liquid film method Demountable cell Powders Total reflectance method ATR 8000A etc KBr pellet method KBr die hydraulic press vacuum pump Nujol method Demountable cell 15 Diffuse reflectance method DRS 8000A_ mix with KBr powder Paper cloth Total reflectance method ATR 8000A etc yarns MIRacle Dura sampler Transmission method Film holder Use a grid polarizer for study of molecular orientation _ Film plastics Total reflectance method ATR 8000A etc MIRacle Dura sampler Transmission method Film holder Use a grid polarizer for study of molecular orientation _ Film method Demountable cell Evaporate the solvent to obtain the film Grind SiC sampler Coating films on Thicker than Total ATR 8000A Measurement to a metals ium reflectance depth of 1 5 of the wavelength method with a KRS 5 prism and to 1 10 with a Ge prism Specular SRM 8000A reflectance method Thinner than Reflec
10. e Single Reflection Horizontal ATR Accessory For most types of samples such as oils powders crystals free standing polymer films pastes and intractable materials the PIKE Technologies MIRacle sampling accessory is suitable This accessory allows for single or multi reflection attenuated total reflectance ATR and specular reflectance measurements In the common configuration the MIRacle accessory is a single reflection ATR sampling accessory This is used for most samples as it allows characterization of thick or strongly absorbing samples without any need for sample preparation To install this accessory place the entire accessory into the sample compartment of the spectrometer with the PIKE label facing outward Align the base plate of the accessory on the spectrometer with the use of the pins on the accessory and the corresponding holes on the spectrometer Fasten the accessory using the captive screw located on the left side of the accessory base plate and tighten by hand Comment BR2 I ve moved the table to the appendix as well Generally the accessory will not require any alignment once installed If however there is a need to realign or fine tune the accessory inform the person in charge The IRSolution software may however run an autoadjustment which will be discussed later 3 1 2 30Spec 30 Degree Specular Reflectance Accessory The Specular Reflectance accessory is most commonly used for thin films of samp
11. e particular sampling accessory to be used with your sample is in place in the sample compartment Without any sample loaded onto the accessory take a background measurement While on the Measure tab of the main window click the BKG button A dialog box will appear requiring you to verify that no sample is in the path of the beam screen shot Confirm that this is so and click the OK button Background measurement will commence and the spectra scanned will be displayed under the View tab while it is being updated in real time while the progress of background measurement will be displayed on the status bar on the lower left corner of the screen screen shot 12 A normal background spectrum will look like the one shown above for measurements taken in air If the background spectrum you obtain differs from this check to make sure there is no sample on the sample holder or that the crystal plate for the MIRacle accessory is thoroughly cleaned refer to the cleaning section if necessary 3 3 3 Sample Preparation and Sampling Procedure 3 3 3 MIRacle Single Reflection Horizontal ATR Accessory As noted above no sample preparation is required for the MIRacle accessory but make sure that the sample is at room temperature Begin by making sure that the swiveling arm of the pressure clamp is detached or directed away from the sample plate and raised at a sufficient height so that it is elevated a good distance from the sample plate sur
12. face figure Once a background spectrum has been obtained see above for instructions load enough sample to fully cover the 1 8mm sampling area in the center of the crystal plate For oils data collection can be done without using the pressure clamp For solids and soft pliable films for which an intimate contact between the sample and the crystal surface is not readily ensured use the pressure clamp If the pressure clamp is not already mounted on the accessory mount it to the backside of the accessory using the two alignment dowel pins on top of the MIRacle accessory base and using a flathead screwdriver fasten the two captive screws on the clamp frame Swivel the arm of the pressure clamp so that the tip is directly above the crystal plate a locking mechanism with an audible click prevents swinging the arm farther than necessary Lower the pressure tip so that it is in contact with the sample by rotating the large black control knob on the clamp column until an audible click is heard This control knob features a ratchet type clutch mechanism that protects the crystal from damage from over pressuring by controlling the maximum allowable pressure applied on the crystal Proceed with data collection 3 3 3 2 30Spec 30 Degree Specular Reflectance Accessory The Specular Reflectance accessory is most commonly used for thin films of samples that do not transmit infrared light to be continued 3 3 3 3 EasiDiff Diffuse Reflectance Acce
13. g 2960 medium to strong 1470 strong 2850 medium to strong 2925 medium to strong 2890 weak 900 2975 3080 C CH 3020 monosubstituted 900 alkenes 990 670 700 cis disubstituted alkenes 965 trans disubstituted 800 840 aromatic alkenes trisubstituted alkenes benzene substituted alkyne benzene aldehyde monosubstituted benzene ortho disubstituted 22 benzene meta disubstituted benzene C C Alkenes acyclic conjugated C C C C benzene Alkyl Halides Alcohols Aldehydes and ee Carboxylic Acids A Disulfides o o Esters o Nitriles Isocyanates Isothiocyanates Diimides Azides Ketenes 23
14. ge cm 1 Min joo 3 3 1 1 1 Measurement Mode In measurement mode select whether the measured spectra are to be displayed in absorbance or transmittance modes Usually IR spectra are displayed in transmittance in absorbance mode This mode also gives the option of obtaining a Power spectrum WHY WHAT FOR 3 3 1 1 2 Apodization The Apodization function selected is a tapering function that smoothes the interferogram obtained by the instrument to zero at the ends of the sampled region It affects the resolution and the signal to noise ratio S N ratio of the spectra so proper selection is necessary Under most common measurements the Happ Genzel is selected For measurements of high resolution Box Car may be selected For small samples requiring high S N ratio SqrTriangle may be selected 3 3 1 1 3 No of Scans The number of scans 1 to 4000 affects the S N ratio of the obtained spectra For a better S N ratio a larger number of scans can be selected at the expense of a longer measurement time Normally the parameter is set to collect 20 scans 3 3 1 1 4 Resolution For solids and liquids a resolution of 4 or 8 cm is sufficient For gas samples some minute absorption peaks may be neglected at these resolutions Therefore a resolution of 0 5 cmt is usually desired Higher resolutions lead to longer measurement times and poorer S N ratios and therefore setting to higher resolutions than necessary is not re
15. les that do not transmit infrared light to be continued 3 1 3 EasiDiff Diffuse Reflectance Accessory to be continued 3 2 Starting up the FTIR system Turn on the spectrometer by pressing the power switch remember the instrument should always be connected to a power source Turn on the computer and monitor attached and log into your GT account Once Windows has completed the PC start up process double click on the JRSolution icon on the desktop to start the FTIR software When available the instrument stores the background spectrum obtained from the latest use Thus usually you will see the following message upon opening the IRSolution software EJ RRcolution Measure fe leis File Edit Measurement AJM Graph Admin Manipulation Manipulation 2 Search Quant Macro Print 3Dim Window Enyironment Help SRDAMAPGARAZARSSHE BAAXE aS mese View BManpuaton Mseach Fon E Finom s oR aA J _ sto _ __Heb View beckoround joe Comment FTIR Measurement I Continuous jimi T w B Datafie C Program Fies Shimadzu IAsohtonidata NT NTOS 17 Auto increment ZA ee at A Previous Background Data pee Aan aa a e E za fi SBKGS02005 lt 3 22 2012 3 01 22 PM gt A Happ Genzel A 2 4000 550 ZE Za J Eas Ws Remove the marked data ix ee 11 28 22M 3727 2012 Log siart user ne 11 28 23 AM 3 27 2012 Intiakong IRA 11 28 28 AM 3 27 2012 Init delay
16. neeseeseeans 8 Bede Date ACQUISTELOM iasan nae a e A aE ected ETE EA AES 8 3 3 1 Setting the Scanning Parameters eesssecssessesseesesssssseeseesssesseesteensesneesneesesseeeseeeteateentennees 8 3 3 2 Obtaining a Background Spectrum ceceeceseesessessesteeseeessseesteeneesneesseeseesteeseeeeenees 12 3 3 3 Sample Preparation and Sampling Procedure ue sssessesstesstecntesneeseeseesteeeeseentees 13 3 4 Data Collect om sircesscccsaccascccscsecscteseccencocacsctcccasncoustoeseeedancesncsssateascessesecstecsceedanttecctacssuscesshcecusessenctasttes 13 3 57 Spectral Manipulation secdaszsccescastessscaiascivsrasccecesazcsecsaadssecsaiesccesncnheaatecxtusdschassasncruaceadtesnsaaatcandaatins 14 3 6 D ta processing tsiesccccewecedicscescteue yeasts oi ddccacusndizsvesecciesuenhteeusscotosen dite vena SEEE EAEra T EEKE e AENT aE 14 3 7 Data templates and printing eecsesssesssessessessesseecssssseesseeneesneesnessssneeseeseeeseeeseesseenteenseaneesess 14 3 8 0 Library searching vrcscsssscccatscosieeseccsseavarescscansesctsasbeccsscissccsensnecsestacccunsiseccuvtsneatuasuender ateacersntaciesetc 14 3 9 Cleaning the Sampling ACCESSOLY sessesessesseessssesssesseesseseseentecntesneesneeseeseesseeestesseenteeneeaneesees 14 4 Common applications for Reynolds Group ssssssessssssssesstesssesteensecneesneeseeseeseeeseeeteeateensesneesees 15 ASU EE A EA T E AE A REAA 15 S Speclal ap plicatl onssas Ea ataa a E E a 15 5 1 Attenuated Total Reflect
17. ssory to be continued 3 4 Data collection Once the sample has been loaded onto the proper sample holder click the Measure tab and then click the Sample button Sample spectra will be collected and once again the progress in measurement will be displayed in the status bar while the real time window will display the sample spectrum in transmittance mode 13 While spectra is being collected the measurement can be paused and restarted Click the Stop button to pause The following dialog box will appear screen shot To stop the measurement and display the spectrum up to the number of scans obtained click the Stop button on the dialog box To stop measurement and abandon the data acquired click the Abort button To resume measurement click the Cancel button Once the measurement is completed the main window will switch to the View tab The spectra will be displayed in two windows with the upper window being the full view of the spectra called the Overview window while the lower window being the Zooming window 3 5 Spectral Manipulation 3 6 Data processing 3 7 Data templates and printing 3 8 Library searching 3 9 Cleaning the Sampling Accessory 14 4 Common applications for Reynolds Group 4 1 5 Special applications 5 1 Attenuated Total Reflectance 5 2 Specular reflectance 5 3 Diffuse reflectance 5 4 6 Transferring data and preparation of spectra using other graphing softwar
18. tfin nteran3 NUM GLP NM zanm However should this not occur you can manually initialize the instrument by clicking the Measurement menu and then choosing Relnitialize nipulation Manipulation 2 Search Quant Macro Print 3Dim Window Enyironment Help 2 aaax a RE Sample Scan 4 ub Background Scan e View AMenpuaton MSearch Fut an S Pi Fom r ER E Monitor Scan Threshold Scan Sanie _ Montor __ Heb View beckoround Stop FTIR Measurement I Continuous fmit T E Diagnostics fie C Program Fies Shimadau IRisoluion data NTWNTOS _ F7 Auto increment AX QuickStart Accessories P Sampler gt lt Auto Adjust Fine t Auto Adjust Coarse ASTM Level Validation JP Validation EP Validation EasyScan ReportViewer PharmaReport ContaminantAnalysis Pertoming diagnostic power measurer Setting aperture open Power diagnostic OK 116 1 179 9 89 INIT success a 11 29 30AM 3 27 2012 Modet IRAffin gy ee a Initializes the FTIR g nteran3 Again the Status window should display the INIT success message upon a successful initialization If a sampling accessory is in place the spectrometer may need to perform an automatic adjustment which resets certain parameters to accommodate the change in the optical path The following dialog box will appear olution data NTSNT O05 mj Auto increment Autoadjust required Perform autoadjustment Cli
19. tion RAS 8000A Use of a grid ium absorption polarizer enhances sensitivity spectrometry about two times Mix with KBr KBr pellet KBr die hydraulic press powder method vacuum pump 16 Diffuse reflectance method DRS 8000A Coating films on Total reflectance method resins Semiconductors ATR 8000A Measurement to a depth of 1 5 of the wavelength with a KRS 5 prism and to 1 10 with a Ge prism Specular reflectance method Direct measurement SRM 8000A Convert a reflection spectrum into an absorption spectrum by the Kramers Kronig method Film holder Liquids Oil content Measures mineral oils only uartz cell measurement Also measures animal plant oils CaF2 cell Nonvolatile Liquid film method Demountable cell organic solvents Solution method Volatile organic Fixed thickness cell sealed liquid solvents cell Rapid Liquid film Demountable cell measurement method Aqueous Above 10 in Liquid film Demountable cell with KRS 5 solutions concentration method Below 10 in ATR method Horizontal type or cylinder concentration internal reflection type ATR attachment Extract solutions Difference Solution Fixed thickness cell spectrometry method Evaporate Diffuse DRS 8000A The sample solution solvent reflectance is supplied dropwise on KBr method powder and measured after
20. with ketones anhydrides fiom asom S aoyimatides asooo o carboxylates salts 1550 1610 cm 1610 cm alcohols phenols low concentration n e 3670 cm 3400 cm carboxylic acids low concentration on 3560 cm soodem bread any 3400 strong 3500 cm 1560 strong 1640 cm secondary amines an y ammonium ions an gt 3000 cm weak to medium multiple broad peaks 20 tertiary 1150 1200 cm 1200 om 1120 on ethers i 1260 cm S 1300 cm Eo ee two bands distinct from ketones which do 1300 cm not possess a C O bond alcohols primary 1040 strong broad 1060 cm 1100 em 1 carboxylic acids 1 often overlapped 1220 cm similar conjugation effects to C O 1700 cm R N C 2165 2110 cm an an an an an isocyanides an all 2030 en E Mee R N C S 2140 1990 cm ordinary 1000 1100 cm chtoroatkanes any 540 260 cm 500 600 on iodoatkanes any sooem mediumiostong nitro compounds aliphatic 1540 om 1380 om 21 y y y y y y y y CEN nitriles 2250 em fluoroalkanes 1 trifluromethyl 1100 two strong broad bands 1200 cm aromatic 1520 lower if conjugated 1350cm Source http en wikipedia org wiki Infrared_spectroscopy_correlation_table update with information from other reliable sources Bond Type Functional Group Absorption Range cm Intensity 1260 strong 1380 weak 2870 medium to stron
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