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1. RES FUJIFILM Corporation FUJIFILM Corporation FUJIFILM Corporation FUJIFILM Corporation m FUJIFILM Corporation FUJIFILM Corporation
2. FUJIFILM Corporation FUJIFILM Corporation FUJIFILM Corporation RA FUJIFILM Corporation m ity FUJIFILM Corporation 2006 2007 897N100125B QuickGene Mini80 User s Manual 2007 6 iii R as QuickGene Mini80 QuickGene Mini80 IN warning WARNING AN BlOHAZARD BIOHAZARD IN caution CA
3. FUJIFILM Corporation QuickGene Mini80 FUJIFILM Corporation xii 897N100125B QuickGene Mini80 User s Manual 2007 6 caution DNA DNA DNA RNA RNA RNA HC RNA RNA Note QuickGene Mini80 CAUTION
4. QuickGene Mini80 AN 4548 FH Be ha HI ef BG SS HI HERR o QuickGene Mini80 QuickGene Mini80 IN saunion QuickGene Mini80 QuickGene Mini80 QuickGene Mini80 E QuickGene Mini80 QuickGene Mini80
5. CAUTION QuickGene Mini80 AN BIOHAZARD N BIOHAZARD 897N100125B QuickGene Mini80 User s Manual 2007 6 xiii FUJIFILM Corporation QuickGene Mini80 QuickGene Mini80 QuickGene Mini80 897N100125B QuickGene Mini80 Users Manual 2007 6 Informations concernant la s curit Ce chapitre d crit les pr cautions de s curit Pour votre s curit et celles des autres veuillez suivre les indications fournies dans les pages suivantes et qui concernant utilisation du QuickGene Mini80 A propos de A Mark V rifiez toutes les descriptions de la ma
6. When the solution in each Cartridge is discharged completely before pressure application automatically stops return the rotary switch to the original position to terminate pressure application Refer to Step 6 Pressure application automatically stops in about one minute 897N100125B QuickGene Mini80 User s Manual 2007 6 19 2 Operation Pressure n Head Pressure Head 20 Rotate up to here Tube Holder 5 6 7 Make sure that the lysate does not remain in the Cartridges Important Make sure that no liquid remains in the Cartridges Residual solution in the Cartridges may cause contamination Note If any solution remains in the Cartridges even after about one minute has elapsed return the rotary switch to the original position Lamp LED ON and then rotate it toward the front side Lamp LED Blink to put pressurized air into the Cartridges again If the rotary switch is returned swiftly to the original position it may result in the splashing of solutions Accordingly rotate the rotary switch slowly If any solution in a Cartridge is not filtered even after pressure application is repeated several times remove the Cartridge by pulling it up vertically and then take appropriate measures referring to the troubleshooting procedures described in the handbook of the Isolation Kits Return the rotary switch to the original position to raise the pressure head
7. A CAUTION Lorsque vous manipulez l un de ces kits consultez le manuel respectif Les r actifs de chaque kit doivent tre manipul s en respectant les consignes de s curit et les pr cautions relatives au kit e L extraction doit tre effectu e dans un laboratoire ou un lieu appropri Kits d isolation Kit ADN sang total Pour ADN Kit ADN tissu Kit plasmides Kit ARN tissu Kit ARN cellules de culture Pour PARN Kit ARN cellules de culture humaines Kit ARN cellules sanguines Note les kits ne sont pas fournis avec QuickGene Mini80 S lectionnez le ou les kits de votre choix et commandez les s par ment propos des r actifs non inclus dans les kits A CAUTION Lorsque vous manipulez l un des r actifs non inclus dans les kits respectez les consignes de s curit et les pr cautions relatives aux r actifs e L extraction doit tre effectu e dans un laboratoire ou un lieu appropri Certains r actifs doivent tre manipul s dans une enceinte pare vent propos de la solution de nettoyage A CAUTION Pour manipuler une solution de nettoyage du QuickGene Mini80 et de ses accessoires respectez les consignes de s curit et les pr cautions relatives la solution Eloignez l thanol de toute source d incendie Il est hautement inflammable AN BIOHAZARD Portez toujours des gants appropri s un masque et des lunettes de
8. EMC FCC Rule Part 15B Class A EN61326 Class A EN61000 3 2 EN61000 3 3 Safety UL61010 1 Second Edition CSA C22 2 No 1010 IEC61010 2001 EN61010 2001 IEC61010 2 081 2001 CE LV directive 73 23 EEC EMC directive 89 336 EEC 897N100125B QuickGene Mini80 User s Manual 2007 6 33 5 Main Specifications FCC WARNING Changes or modifications not expressly approved by the party responsible for compliance could void the user s authority to operate the equipment This equipment has been tested and found to comply with the limits for a Class A digital device pursuant to Part 15 of the FCC Rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instruction manual may cause harmful interference to radio communications Operation of this equipment in a residential area is likely to cause harmful interference in which case the users will be required to correct the interference at their own expense Warning This is a Class A product of Electromagnetic Interference EMI standard In a domestic environment this product may cause radio interference in which case the user may be required to take adequate measures This Class A digital apparatus complies with Canadian ICES 003 Cet appareil num rique de la class A est comforme
9. Operating state is indicated by the lamp LED When the power is turned on the lamp LED lights During pressure application the lamp LED blinks The main unit contains a pump for pressurizing air a sensor for detecting start stop of pressure application and a circuit for controlling the duration of pressure application Pressure Unit Puts pressurized air into the Cartridges through the pressure heads When the rotary switch is turned on the pressure heads move down to put pressurized air into Cartridges Rack Unit The rack unit is comprised of the cartridge holder and tube holder Lysate Wash Buffer Elution Buffer put in a Cartridge passes through the filter in the Cartridge by pressurized air and filtered solution drops into a Waste Tube or Collection Tube Note Pressure application of lysate and wash and that of elution operation are switched by moving the position of the cartridge holder manually mLamp LED indication information The following describes the lamp LED indications Lamp LED Indication Description ON Indicates that the power is ON and pressure application can be performed Blink Indicates that pressure application is in progress Note When pressure application is completed the lamp LED indication returns to the ON state 897N100125B QuickGene Mini80 User s Manual 2007 6 1 Setting Up 1 6 Basic Operation The following describes the working flow of Quic
10. Die FUJIFILM Corporation bernimmt keine Haftung f r Ger te Ausf lle und Besch digungen die auf Naturkatastrophen wie Feuer Erdbeben berschwemmung oder Blitzschlag zur ckzuf hren sind e Kein Teil dieser Anleitung darf ohne Erlaubnis der FUJIFILM Corporation reproduziert werden m Warenzeichen Firmen und Produktnamen sind Warenzeichen oder eingetragene Warenzeichen der jeweiligen Inhaber Alle Rechte vor behalten Copyright FUJIFILM Corporation 2006 2007 897N100125B QuickGene Mini80 User s Manual 2007 6 vii viii Warnhinweise Diese Anleitung enth lt wichtige Informationen zur sicheren Verwendung von QuickGene Mini80 Lesen Sie diese Informationen sorgf ltig durch bevor Sie QuickGene Mini80 in Betrieb nehmen Die Bedeutung der verschiedenen Sicherheitshinweise wird im Folgenden erl utert A WARNING WARNING weist auf gef hrliche Betriebsbedingungen hin die zu ernsthaften Verletzungen oder Verletzungen mit Todesfolge f hren k nnen AN BIOHAZARD BIOHAZARD kennzeichnet die Gef hrdung durch biologische Agenzien oder Verh ltnisse die infekti s oder gesundheitssch dlich sein k nnen CAUTION weist auf gef hrliche Betriebsbedingungen hin die zu CAUTION A R a leichten bis mittleren Verletzungen f hren k nnen CAUTION kann dar ber hinaus das Risiko einer Sachbesch digung kennzeichnen Important Important kennzeichnet wichtige Bedienungshinweise sowie zu unte
11. hazard symbol m Clauses d exclusion e Le contenu de ce manuel peut tre mis jour sans pr avis FUJIFILM Corporation ne peut tre tenu pour responsable de la violation aux copyrights de tiers ou d autres droits manant de l utilisation d une information quelconque contenue dans ce manuel e FUJIFILM Corporation ne peut tre tenu pour responsable d une d faillance ou de dommages de l appareil caus s par l installation la relocalisation la modification la maintenance et les r parations effectu es par des tiers autres que FUJIFILM Corporation ou des agences agr es par FUJIFILM Corporation e FUJIFILM Corporation ne peut tre tenu pour responsable d une d faillance ou de dommages dus l utilisation de produits tiers e FUJIFILM Corporation ne peut tre tenu pour responsable d une d faillance ou de dommages de l appareil dus la modification la maintenance les r parations etc utilisant des pi ces de rechange qui ne sont pas des pi ces d origine sp cifi es par FUJIFILM Corporation FUJIFILM Corporation ne peut tre tenu pour responsable d une d faillance ou de dommages de l appareil dus la non observation des proc dures de fonctionnement et des pr cautions d crites dans ce manuel e FUJIFILM Corporation ne peut tre tenu pour responsable d une d faillance ou de dommages de l appareil au cas o il est utilis dans un environnement qui ne r pond pas aux conditions de fonctionnement a
12. un incendie ou une d charge lectrique En cas de surchauffe de l appareil ou s il d gage de la fum e ou une odeur bizarre d branchez imm diatement le c ble d alimentation e N essayez en aucun cas de modifier le QuickGene Mini80 sans l autorisation du fabricant Cela pourrait entra ner un incendie ou une d charge lectrique Ne placez et ne faites tomber aucun objet sur le QuickGene Mini80 De m me vitez de lui faire subir des chocs ou des coups cela pourrait entra ner des d faillances et un mauvais fonctionnement du QuickGene Mini80 Si un quelconque liquide s introduit dans l appareil pongez le avec une serviette en papier etc Sinon le QuickGene Mini80 pourrait tre endommag Lorsque vous remplacez les t tes de pression veillez porter des gants Si de l thanol est renvers l int rieur du dispositif debranchez immediatement le cable d alimentation e Des r parations du QuickGene Mini80 ne doivent tre effectu es que par des agences sp cifiquement autoris es par FUJIFILM Corporation e Seules les pi ces d tach es originales provenant de FUJIFILM Corporation doivent tre utilis es e Si ce mat riel est utilis de mani re non sp cifi e par le fabricant la protection fournie par le mat riel peut s en trouver diminu e 897N100125B QuickGene Mini80 User s Manual 2007 6 XV propos des kits d isolation appel s ci apr s kit kits dans cette section
13. Extraktion sollte in einem geeigneten Laboratorium oder einem entsprechendem Arbeitsplatz durchgef hrt werden Isolations Kits DNA Vollblut Kit F r DNA DNA Gewebe Kit Plasmid Kit RNA Gewebe Kit RNA Kulturzellen Kit F r RNA RNA Kulturzellen HC Kit RNA Blutzellen Kit Note Die Kits werden nicht mit QuickGene Mini80 ausgeliefert W hlen Sie die gew nschten Kits aus und bestellen Sie sie getrennt ber nicht in den Kits enthaltene Reagenzien A CAUTION Beim Umgang mit einem Reagens das nicht in den Kits enthalten ist sind die Sicherheitsinformationen und Vorsichtsma regeln des jeweiligen Reagens zu befolgen e Die Extraktion sollte in einem geeigneten Laboratorium oder an einem entsprechenden Arbeitsplatz durchgef hrt werden Mit einigen Reagenzien sollte in einer Abzugskammer gearbeitet werden ber Reinigungs Waschl sung A CAUTION Bei der Anwendung von Reinigungs Waschl sungen beim QuickGene Mini80 und den Zubeh rteilen sind die Sicherheitsinformationen und Vorsichtsma regeln f r die L sung zu beachten Halten Sie Ethanol fern von Feuer Es ist leicht entflammbar AN BIOHAZARD Beim Reinigen Waschen immer geeignete Handschuhe einen Mundschutz und eine Schutzbrille tragen ber die Proben AN BIOHAZARD Beim Umgang mit infekti sen Proben immer geeignete Handschuhe einen Mundschutz und eine Schutzbrille tragen XX 897N100125B Qu
14. Manual 2007 6 35 A3 A4 36 About Services Before you ask for device repairs refer to 4 Troubleshooting P 31 to check the problems If the problems cannot be solved contact your local representative or the relative customer support Notes for Transporting Shipping QuickGene Mini80 When transporting shipping QuickGene Mini80 to a new location perform the following tasks the power cable is damaged this may cause a device failure fire injury A Before carrying QuickGene Mini80 disconnect the plug from the outlet If Warning or electric shock When transporting shipping QuickGene Mini80 be sure to perform the following tasks Remove all attached parts tube holder and cartridge holder from QuickGene Mini80 Place the cushion removed at the time of unpacking onto the pressure heads and then secure the cushion and rotary switch with tape Refer to 1 3 Remove the Packing Materials P 4 After completing the above tasks pack QuickGene Mini80 in its original shipping box or some other equivalent box 897N100125B QuickGene Mini80 User s Manual 2007 6 Nucleic Acid Isolation Device QuickGene Mini80 Users Manual 897N100125B Date of issuance June 2007 FUJIFILM Corporation Printed in Japan e The contents of this manual may be updated without notice e The FUJIFILM Corporation assumes no liability for damages to third party copyrights or other rights arising from the use of any informatio
15. Mini80 and Accessories pp 3 1 3 Remove the Packing Materials 4 1 4 Overall View sm hed a nn tenue 5 T5 B sic Functi ns enge nd ds REE 8 1 6 B asic Oper ation 2 unn en 9 1 7 Precautions on Transportation 10 1 8 Precautions on Storage 11 2 Operation 2 1 Turning the Power ON and OFF 13 2 2 PreparatiON dd drt ira 15 2 35 VEXUPACTION 222 223 302 Baal nc es A ne aca teekente cee 16 3 Maintenance 3 1 Cleaning QuickGene Mini80 0 27 3 2 Cleaning the Parts nes 28 3 3 How to Clean Replace the Pressure Heads uunssuneunnnnnnnnnnnnnnn 28 4 Troubleshooting 5 Main Specifications Appendix A A1 Replacement Optional Parts 35 A 2 Product Guarantees 35 3 About SOrVICES 25e sn inanin aeii sinik aidea 36 A4 Notes for Transporting Shipping QuickGene Mini80 36 897N100125B QuickGene Mini80 User s Manual 2007 6 XXV xxvi 897N100125B QuickGene Mini80 User s Manual 2007 6 1 Setting Up 1 Setting Up 1 1 897N100125B QuickGene Mini80 User s Manual 2007 6 1 This chapter explains how to set up QuickGene Mini80 Operating Environment Use Conditions This section explains the operating environment and use conditions of QuickGene Mini80 Operating environment A Warning Do not use QuickGene Mini80 toa location where it can be splashed with water It may cause a device failure fire or electric shock When relocat
16. THE RISK F FRE OR ELECTRICAL TO REDUCE THE RISK OF FIRE OR ELECTRICAL OCK DO NOT EXPOSE IS APPARATUS TO N OR MOISTURE ADAPTOR MODEL SQNSEW12P 05 INPUT TOOV 240V 075A 0 4A S TUT 12 AA SHOCK DO NOT EXPOSE THIS APPARATUS TO a Pe Oge eee e Nu wove Quick Gene Mini80 The E mark is the mark of the Class Il device ao m This mark y a represents iS DC Input 897N100125B QuickGene Mini80 User s Manual 2007 6 1 Setting Up MCartridge holder Cartridge CA Release Lever Name Description Release Lever Releases the Cartridges by sliding the lever All Cartridges are released by sliding the lever to the right hand end To release the No 1 to No 4 Cartridges only stop the lever at the center position Note Before setting Cartridges make sure that the release lever is set in the left hand position MTube holder Waste Tube WT Tube CT I ea Adapter Cartridge Holder Insertion Section E Insertion section for elution W Insertion section for wash 897N100125B QuickGene Mini80 User s Manual 2007 6 1 Setting Up Label ON OFF Mark Waste Fluid Scale WASH Label Name Description ON Off Mark ON Mark Rotating the switch to the ON Mark
17. User s Manual 2007 6 21 2 Operation Rotate up to here 11 When wash process is completed return the rotary switch to the original position to raise the pressure heads Pressure Head a Important Make sure that no liquid remains in the Cartridges Residual solution in the Cartridges may cause contamination Ci CE CT Note Do not pull out the tube holder and cartridge holder while the pressure heads are lowered 12 Pull out the tube holder and cartridge holder Tube Holder BPrecautions for Putting DNase into Cartridges Apply DNase directly onto the filter in each Cartridge Be careful not to touch the filter with the pipette tip lt Elution gt 13 Remove the cartridge holder and then insert it into the Elution Position E of the tube holder Cartridges are positioned above the Collection Tubes Important Ensure that the tube holder is set in the Elution Position E before applying Elution Buffer Note When moving the cartridge holder do not slide the release lever Tube Holder 22 897N100125B QuickGene Mini80 User s Manual 2007 6 2 Operation 14 Apply Elution Buffer to each Cartridge Use a pipette to apply Elution Buffer to the Cartridges In this operation take care not to touch the filter in the Cartridge the Cartridge itself or the cartridge holder Note If any Elution Buffer is left on the rim of a Cartridge or on the cartridge holder wipe it off w
18. a la nomal NMB 003 du Canada 34 897N100125B QuickGene Mini80 User s Manual 2007 6 Appendix A 1 Replacement Optional Parts Name Rack Set P N 802Y100014 Name Cartridge Holder P N 362Y 100003 Name Tube Holder P N 353Y100011 Name Tube Adapter P N 360N100018B Single item 360Y 100069 8 pcs Name Pressure Heads P N 371Y100005 Single item 371Y100010 8 pcs Name AC Adapter P N 12580319 SQN36W12P 05 125N100014 SQN36W12P 08 Name Power Cable P N 136Y100273 for US Canada D LL Name Cover P N 350Y100419 Name Tube Racks 4 P N 15557927 The rack set is comprised of one cartridge holder one tube holder and eight tube adapters A 2 Product Guarantees e The guaranty period shall be one year from the delivery of the product Any part which fails to function properly under normal use provided that all warnings and cautions in this manual are observed during the guarantee period will be repaired by the FUJIFILM Corporation free of charge If the problem is due to the causes listed below some charge may be applied for the repair even within the guarantee period Any failures due to improper use or that are affected by other devices except for those approved by the FUJIFILM Corporation Any malfunctions or damages during transportation or due to dropping of the product by a user 897N100125B QuickGene Mini80 User s
19. mask and safety goggles during A BIOHAZARD any biohazardous operations in extraction process Even when touching the device after any operation with a biohazard risk wear appropriate gloves and a mask since the device may be contaminated goggles if required by the operation In the operation from preparing samples to extraction completion be careful not to contaminate the samples with Important Before starting extraction put on appropriate gloves a mask and safety 2 sweat saliva etc 2 1 Turning the Power ON and OFF This section explains how to turn the power of QuickGene Mini80 on and off Note Since QuickGene Mini80 is not equipped with a power switch the power is turned on off by connecting disconnecting the power cable MTurning on the power 1 Make sure that the AC adapter and power cable are connected securely and the plug of the AC adapter is securely connected to the QuickGene Mini80 Note QuickGene Mini80 is a Class Il device Be sure to use the attached AC adapter The El mark is the mark of the Class Il device 2 Connect the plug of the power cable to the outlet The QuickGene Mini80 turns on and the lamp LED lights Plug 897N100125B QuickGene Mini80 User s Manual 2007 6 13 2 Operation MTurning off the power 1 Disconnect the plug of the power cable from the outlet The lamp LED turns off and then the QuickGene Mini80 turns off Note Do not place an
20. return the rotary switch to the original position to terminate pressure application The extracted sample is eluted into the Collection Tube and the waste is discharged into the Waste Tube 897N100125B QuickGene Mini80 User s Manual 2007 6 9 1 Setting Up 1 7 Precautions on Transportation When transporting QuickGene Mini80 note the following points Be sure to hold the handles Do not hold the rotary switch 1 Do not hold the tray Do not insert your hands into the pressure head section Remove the tube holder before transportation Be sure to hold the handles 10 897N100125B QuickGene Mini80 User s Manual 2007 6 1 Setting Up 1 8 Precautions on Storage When storing QuickGene Mini80 note the following points Do not keep it with the rotary switch adjusted to the position of turning on with Cartridges set in the cartridge holder If not the rubber packing on the pressure heads may become deformed and proper operation cannot be guaranteed Put the supplied cover on QuickGene Mini80 When the outside of the body is heavily soiled EI clean it before fitting the cover e Wear appropriate gloves when handling the cover 897N100125B QuickGene Mini80 User s Manual 2007 6 11 1 Setting Up 12 897N100125B QuickGene Mini80 User s Manual 2007 6 2 Operation 2 Operation This chapter explains how to operate QuickGene Mini80 and shows the flow of extraction Always wear appropriate gloves a
21. risque de dommage mat riel Important Important signale des remarques importantes concernant l utilisation et les actions interdites Note Note les remarques concernent les proc dures respecter elles fournissent aussi des informations compl mentaires 897N100125B QuickGene Mini80 User s Manual 2007 6 Einf hrung Wir danken Ihnen f r den Kauf des Ger ts zur Nukleins ureisolierung QuickGene Mini80 im Folgenden als QuickGene Mini80 bezeichnet Mit QuickGene Mini80 k nnen DNA und RNA leicht extrahiert werden Diese Anleitung enth lt wichtige Informationen zur sicheren Verwendung von QuickGene Mini80 Lesen Sie die Anleitung vor der Verwendung des Ger ts sorgf ltig durch Bewahren Sie diese Anleitung nach dem Lesen zum sp teren Nachschlagen auf Hinweise zu infekti sen Abf llen Befolgen Sie bei der Handhabung oder Entsorgung von infekti sen Abf llen die Laborrichtlinien sowie die geltenden Gesetze und Vorschriften und f hren Sie die Verbrennung Einschmelzung Sterilisation und oder Desinfektion entsprechend durch Wenn Sie die Entsorgung durch Dritte ausf hren lassen beauftragen Sie Universal biological zugelassene Entsorgungsfirmen f r medizinischen Sonderm ll und stellen Sie hazard symbol ihnen die relevanten Informationen zur Sonderm ll Abfallbehandlung zur Verf gung m Ausschlussklauseln e Der Inhalt dieser Anleitung kann jederzeit ohne Vorank ndigung ge ndert werden D
22. starts pressure application OFF Mark Rotating the switch to the OFF Mark finishes pressure application WASH Label Indicates that tube holder and cartridge holder are set in the Wash Position W correctly When the tube holder and cartridge holder are set in the Elution Position E this label is hidden below the tube holder Waste Fluid Scale The solution level changes according to the number of wash processes L Pressure application of lysate completed W1 First wash process completed W2 Second wash process completed W3 E Third wash process completed After wash processes are completed move the cartridge holder to the Elution Position E to perform elution process mCover Description This cover is for storage purposes Caution Do not operate the QuickGene Mini80 with the cover on Always put the cover on the QuickGene Mini80 after cleaning the outside of the body refer to P 27 Do not use the cover inside out BAIOHAZARD MARK A gionazaro Wear appropriate gloves when handling the cover 897N100125B QuickGene Mini80 User s Manual 2007 6 1 Setting Up 1 5 Basic Functions The following describes the main components of QuickGene Mini80 Pressure Unit Main Unit Rack Unit Component Function Main Unit Starts stops putting pressurized air into Cartridges by operating the rotary unit Pressure application automatically stops in about one minute
23. the right hand end all Cartridges will fall down To release the No 1 to No 4 Cartridges only stop the release lever at the center position In this case to release the remaining Cartridges slide the release lever from the center position to the right hand end When you release your finger pressure from the release lever the release lever returns to the left hand end Note Dispose of the Cartridges according to appropriate laws and rules Dispose of infectious A BIOHAZARD waste according to your biological safety manuals 2 Operation lt gt Collection Tube 2 Remove the Collection Tubes Tube Adapter U When using Collection When using commercially 2 Tubes included in the Note When the Tube Rack is available 1 5 ml micro not used remove the Isolation Kits or he ee Tubes cole con IUNES aner putting Caps on them into the Tube Rack optional The eight Caps included and then put Caps included in in the Isolation Kits can the Isolation Kits on the be detached by hand 1 5 ml Micro Tube Collection Tubes tubes Put caps on 1 5 ml micro tubes and then remove them Collection Tube Cap 3 Remove the Waste Tubes Note Dispose of the Waste Tubes and waste solution according to appropriate laws and rules Dispose of infectious BIOHAZARD Waste according to your biological safety manuals Waste Tube Specified Waste Containers Example 897N100125B QuickGene Mini8
24. 0 User s Manual 2007 6 25 2 Operation 26 897N100125B QuickGene Mini80 User s Manual 2007 6 3 Maintenance 3 Maintenance To use QuickGene Mini80 properly check whether or not each part is clean and the pressure heads are in normal condition before use If necessary perform cleaning and or part replacement referring to the descriptions in this chapter 3 1 Cleaning QuickGene Mini80 This section explains how to clean QuickGene Mini80 BIOHAZARD When cleaning QuickGene Mini80 wear appropriate gloves a mask and safety goggles QuickGene Mini80 and disconnect the power plug from the outlet Otherwise it may cause an electric shock 3 Body outside Before starting the maintenance work turn off the power of A Warning Pressure H ad Body inside Tray Parts to clean Cleaning methods Body outside Remove dust gently with a dry soft cloth If the outside of QuickGene Mini80 is heavily soiled or if any samples that may cause infection are adhered to the outside of the QuickGene Mini80 wipe with a soft paper tissue soaked with 0 5 sodium hypochlorite solution or ethanol Body inside Remove dust gently with a dry soft cloth If the inside of QuickGene Mini80 is heavily soiled or if any samples that may cause infection are adhered to the inside of the QuickGene Mini80 wipe with a soft paper tissue soaked with 0 5 sodium hypochlorite solution or ethanol Note If the tray and its circumf
25. 897N100125B 2007 6 Life Science FUJIFILM Nucleic Acid Isolation Device QuickGene Mini80 Extraction Flow Preparation Extraction with QuickGene Mini80 Pages 16 to 25 ing Applying lysate into the Cartridges repare lysate us Wash Elution of DNA the Isolation Kits RNA Third Edition Introduction Thank you for purchasing the Nucleic Acid Isolation Device QuickGene Mini80 hereafter called QuickGene Mini80 DNA or RNA can be easily extracted by using QuickGene Mini80 This guide contains important information regarding the safe use of QuickGene Mini80 Read this manual carefully before using the device Keep this manual after reading it for reference whenever necessary About Infectious Wastes When handling or disposing of infectious materials follow the laboratory guideline or the law regarding infectious waste to perform proper incineration fusion sterilization and or disinfection When you use a third party to dispose of it outsource this work an operator licensed to handle medical waste subject to special control and give them the Universal biological manifest of the medical waste at the same time hazard symbol m Exclusion clauses The contents of this manual may be updated without notice e The FUJIFILM Corporation assumes no liability for infringement of third party copyrights or other rights arising from the use of any information in this manual e The FUJIFILM Corporation as
26. Tubes and Cartridges Important Put the tubes into the tube holder Cartridge in the order of the number on the Holder tube holder Insert the Cartridges and Tubes starting from the left in O OOO Tube Holder order If they are not in order 1 2 3 4 5 6 7 8 Buffer and or lysate may spill This results in an extraction failure and Buffer and or lysate becoming waste 0000 OK OK OK 1000 mExtraction lt Applying Lysate to Cartridges gt 1 Apply Iysate to each Cartridge Use a pipette to transfer the Iysate into the Cartridges In this operation take care not to touch the filter in the Cartridge the Cartridge itself or the cartridge holder Note If any Iysate is left on the rim of a Cartridge or on the cartridge holder wipe it off with a soft paper tissue 18 897N100125B QuickGene Mini80 User s Manual 2007 6 2 Operation 2 Set the tube holder and cartridge holder into the QuickGene Mini80 Important After setting it check the following The cartridge holder is placed against the stopper inside the QuickGene Mini80 The WASH label can be seen Tube Holder WASH Label Cartridge Holder Rotary Switch 3 Rotate the rotary switch toward the front side to lower the pressure heads Pressure Head E Cartridge Rotate up to here Lamp LED Blink 4 The lamp LED blinks and pressurized air is put into the Cartridges Note
27. UTION CAUTION Important Important Note Note 897N100125B QuickGene Mini80 Users Manual 2007 6 Introduction Merci d avoir achet le Dispositif d isolation d acide nucl ique QuickGene Mini80 appel ci apr s QuickGene Mini80 L extraction de l ADN ou l ARN peut tre facilement effectu e l aide du QuickGene Mini80 Ce guide contient des informations importantes concernant l utilisation en toute s curit du QuickGene Mini80 Lisez attentivement ce manuel avant d utiliser l appareil Apr s lecture conservez ce manuel pour pouvoir vous y r f rer ult rieurement si n cessaire propos des d chets infectieux Lorsque vous manipulez ou mettez au rebut des produits infectieux veillez respecter les directives du laboratoires ou la loi sur les d chets infectieux pour en effectuer l incin ration la fusion la st rilisation et ou la d sinfection correctes Lorsqu un tiers est charg de la mise au rebut veillez la confier une entreprise habilit e traiter les produits m dicaux sujets contr le sp cifique et fournir en Universal biological m me temps le manifeste des produits concern s
28. ailures Phenomenon Cause Appropriate action The lamp LED does not light e Connect the power plug to the outlet completely Connect the AC adapter to QuickGene Mini80 completely The power plug is not connected to the outlet e The AC adapter is not connected to QuickGene Mini80 e The power cable is not connected to the AC adapter Connect the power cable to the AC adapter completely Insert the Cartridges completely into the cartridge holder e Set the Collection and Waste Tubes corresponding to the position of the Cartridge to be inserted Cartridges are incorrectly inserted into the cartridge holder The solution put in Cartridges does not steadily drop into the Collection or Waste Tubes Replace the pressure heads with new ones P 28 Pressure heads have become deformed or hardened Install the pressure heads correctly P 28 Clean the cartridge holder P 28 Pressure heads are incorrectly installed Cartridges cannot be The cartridge holder is released held properly soiled Pressure application does not start even after rotating QuickGene Mini80 may have a mechanical or Contact your local representative the rotary switch toward the electrical problem front side QuickGene Mini80 emits an abnormal noise QuickGene Mini80 may Contact your local representative have a mechanical or electrical problem Turn off the pow
29. ardless of the application e It is the user s responsibility to validate the performance of the Isolation Kits and QuickGene Mini80 e QuickGene Mini80 is a device for research and study only When you use QuickGene Mini80 read this manual thoroughly and follow the safety management regulations of your facilities laboratory 897N100125B QuickGene Mini80 User s Manual 2007 6 xi QuickGene Mini80 A nr IN warnne FE E A QuickGene Mini80
30. ch kit should be handled by observing the safety information and precautions regarding the kit Extraction should be performed in an appropriate laboratory or workplace isolation kits DNA whole blood kit For DNA DNA tissue kit Plasmid kit RNA tissue kit RNA cultured cell kit For RNA RNA cultured cell HC kit RNA blood cell kit Note The kits are not supplied with QuickGene Mini80 Select the desired kit s and order it them separately About Reagents Not Included in the Kits When handling any reagent not included in the kits observe the safety CAUTION information and precautions of the reagent Extraction should be performed in an appropriate laboratory or workplace Some reagents should be handled in a draft chamber About Cleaning Washing Solution For handling any cleaning washing solution for the QuickGene Mini80 CAUTION z 2 and accessories observe the safety information and precautions regarding the solution e Keep ethanol away from fire It is highly flammable AN BIOHAZARD Always Wear appropriate gloves a mask and safety goggles when cleaning washing About Samples AN BIOHAZARD Always wear appropriate gloves a mask and safety goggles etc when handling any infectious samples x 897N100125B QuickGene Mini80 User s Manual 2007 6 Product Use Limitations The FUJIFILM Corporation assumes no liability for results reg
31. d Kanada Verwenden Sie au erhalb der USA und Kanada ein Netzkabel das Ihrer Landesnorm entspricht oder wenden Sie sich an Ihren lokalen H ndler Verwenden Sie das mitgelieferte Netzteil Verwenden Sie das beiliegende nur f r amerikanische und kanadische Modelle geeignete Netzkabel nicht f r andere Produkte Betreiben Sie QuickGene Mini80 ausschlie lich mit der am Ger t angegebenen Netzspannung berlasten Sie die Steckdose nicht durch Anschlie en von zu vielen Ger ten Verwenden Sie QuickGene Mini80 nicht mit einem besch digten Netzstecker oder einer lockeren Steckdose Falls die Kontakte des Netzsteckers oder die Steckdose staubig sind entfernen Sie den Staub mit einem trockenen Tuch Wenn Sie den Netzstecker aus der Steckdose ziehen fassen Sie stets den Netzstecker direkt an Ziehen Sie nicht am Netzkabel Ziehen Sie vor der Durchf hrung von Wartungsarbeiten den Netzstecker ab Fassen Sie den Netzstecker bei Gewitter nicht an e Versch tten Sie keine Fl ssigkeiten ber QuickGene Mini80 Stellen Sie keine Fl ssigkeitsbeh lter auf QuickGene Mini80 ab Dadurch k nnte es zu einem Ausfall des Ger tes zu einem Brand oder Stromschlag kommen e Wenn sich das Ger t berhitzt oder wenn Rauch oder ungew hnliche Ger che auftreten ziehen Sie sofort das Netzkabel ab 897N100125B QuickGene Mini80 User s Manual 2007 6 JX CAUTION Versuchen Sie nicht QuickGene Mini80 ohne die Erlaubnis des Herste
32. enance disconnect the power plug from the outlet Do not touch the power plug when you hear the crash of thunder Do not pour any liquid on QuickGene Mini80 Do not place any objects containing liquid on QuickGene Mini80 Doing so may cause a device failure fire or electric shock In the event the device overheats starts to smoke or smells strange immediately unplug the power cable Never attempt to remodel QuickGene Mini80 without the manufacturer s permission Doing so may cause fire or electric shock Do not place or drop objects on QuickGene Mini80 Also refrain from bumping or knocking it as doing so may cause a failure or malfunction of QuickGene Mini80 If any liquid materials are left inside the device wipe it up a soft paper tissue etc Otherwise QuickGene Mini80 may be damaged When replacing the pressure heads be sure to wear gloves If ethanol is spilled inside the device immediately unplug the power cable Repairs to QuickGene Mini80 should only be performed by such agencies as are specifically authorized by the FUJIFILM Corporation Only original the FUJIFILM Corporation replacement parts should be used If the equipment is used in a manner not specified by the manufacturer the protection provided by the equipment may be impaired About isolation kits Referred to as kit kits hereafter in this section A CAUTION e When handling any of the kits refer to the respective handbook Reagents in ea
33. er immediately by disconnecting the power cable from the outlet and then contact your local representative QuickGene Mini80 emits an abnormal smell 897N100125B QuickGene Mini80 User s Manual 2007 6 31 4 Troubleshooting 32 897N100125B QuickGene Mini80 User s Manual 2007 6 5 Main Specifications 5 Main Specifications The following describes the main specifications of QuickGene Mini80 amp Main specifications Items Specifications Model name QuickGene Mini80 Dimensions 276 mm W x 219 mm D x 176 mm H Weight 2 8 kg including the main body rack set AC adapter and power cable The power cable is provided only for US and Canada Samples Max 8 samples can be processed simultaneously Range of temperature Temperature 15 30 C and relative humidity Humidity 30 80 RH non condensing mPower supply conditions AC Adapter Items Specifications Rated input voltage AC 100 to AC 240 V Voltage fluctuation range 10 Phase Single Power frequency 50 60 Hz Rated input current 0 75 to 0 4 A Output voltage DC 12 V Rated output current 3A Main Unit Items Specifications Rated voltage DC 12 V 5 Rated current 0 5A mEnvironmental conditions Items Specifications Usage environment Indoor use only Overvoltage category Transient overvoltage category II definition Pollution degree Pollution Degree 2 IP IP xO
34. erence are heavily soiled remove the tray before cleaning Pressure Heads Wipe with a soft paper tissue soaked with ethanol Note If the pressure heads are heavily soiled or any abnormal conditions damage deformation hardening etc are found replace them with new ones P 28 A Caution Keep ethanol away from fire when using It is highly flammable 897N100125B QuickGene Mini80 User s Manual 2007 6 27 3 Maintenance 3 2 Cleaning the Parts The following table describes how to clean the parts Parts Cleaning methods Cartridge Holder Clean the parts using one of the following methods Tube Holder e Clean with neutral detergent and then wash out with distilled water Tube Adapter Next soak into 3 hydrogen peroxide solution at room temperature for 10 minutes Lastly wash the solution out with DEPC water and dry the parts Soak in a 0 1N sodium hydroxide 0 1 EDTA solution overnight and then wash the solution out with DEPC water and dry the parts e Clean the parts with neutral detergent then wash out with 0 5 sodium hypochlorite solution Next wash the solution out with DEPC water and then dry the parts 3 3 3 How to Clean Replace the Pressure Heads Heavily soiled damaged deformed or hardened pressure heads may cause contamination or insufficient pressure application resulting in extraction failure Accordingly if any abnormal conditions are found in the pressure heads replace them p
35. he cartridge holder from the tube Cartridge Holder holder Note Pull up the cartridge holder vertically to prevent it becoming caught in the tube holder 16 897N100125B QuickGene Mini80 User s Manual 2007 6 2 Operation 4 Set Collection Tubes and Waste Tubes into Collection Tube the tube holder Waste Tube Tube Holder When using Collection Tubes When using commercially available included in the Isolation Kits 1 5 ml micro tubes as collection Set a tube adapter into the tube holder tubes as shown and then insert a Collection Set 1 5 ml micro tubes so that the cap is Tube into the tube adapter positioned as shown Tube adapters are not used gt Collection Tube 1 5 ml Micro Tube Cap Tube Adapter Cartridge Holder 5 Insert the cartridge holder into the Wash Position W of the tube holder Tube Holder 897N100125B QuickGene Mini80 User s Manual 2007 6 17 2 Operation 6 Set Cartridges into the cartridge holder Cartridge Important Make sure that the release lever is positioned at the left hand end If any Cartridges are set without the release lever positioned at the left hand end the Cartridges will drop into the Waste Tubes e Align the fins on the Cartridge with the grooves in the cartridge holder and then push in the Cartridge e Set Cartridges into the cartridge holder in the order of the number on the cartridge holder 7 Check the location of the
36. he temperature and humidity are kept within the specified range far from water taps water heaters humidifiers air conditioners and heaters 1 Setting Up e A location far from objects which generate strong magnetic fields motors transformers TV audio speakers magnets etc Bringing QuickGene Mini80 close to any type of magnetic field may cause a malfunction mUse conditions EN Items Specifications Temperature C During operation 15 30 During downtime 0 55 Humidity RH During operation 30 80 During downtime 10 80 Max wet bulb During operation 29 non condensing temperature C During downtime 29 non condensing Temperature gradient C hr 12 or less non condensing Humidity gradient RH day 30 or less non condensing Altitude operating m 1600 or less 2 897N100125B QuickGene Mini80 User s Manual 2007 6 1 Setting Up 1 2 Checking QuickGene Mini80 and Accessories Check that the following are included in the package Contact your local representative if you notice any missing part s e QuickGene Mini80 e QuickGene Mini80 User s AC Adapter ER e Power Cable e Cover Only US Canada e Tube Adapter x8 A Caution Note Use an appropriate power cable by following the descriptions below US Canada Use the power cable included in the package Other countries The power cable is not included in the package Prepare the power cab
37. ickGene Mini80 User s Manual 2007 6 Einschrankungen der Produktverwendung e Die FUJIFILM Corporation bernimmt keine Haftung f r die Ergebnisse unabh ngig von der Anwendung Es liegt in der Verantwortung des Anwenders die Eignung des Isolations Kits und QuickGene Mini80 zu berpr fen e QuickGene Mini80 ist nur f r den Einsatz in Forschung und Lehre vorgesehen Wenn Sie QuickGene Mini80 einsetzen lesen Sie diese Anleitung sorgf ltig durch und befolgen Sie die Sicherheitsbestimmungen Ihrer Einrichtung Labor 897N100125B QuickGene Mini80 User s Manual 2007 6 xxi xxii 897N100125B QuickGene Mini80 User s Manual 2007 6 INDEX 1 Setting Up This chapter explains how to set up QuickGene Mini80 2 Operation This chapter explains the extraction procedures 3 Maintenance This chapter explains how to maintain QuickGene Mini80 4 Troubleshooting This chapter explains the procedures that should be taken when you suspect device failures 5 Main Specifications This chapter explains the basic specifications of QuickGene Mini80 Appendix A This appendix describes after sales services and points to note for transportation 897N100125B QuickGene Mini80 User s Manual 2007 6 xxiii xxiv 897N100125B QuickGene Mini80 User s Manual 2007 6 Contents 1 Setting Up 1 1 Operating Environment Use Conditions pe 1 1 2 Checking QuickGene
38. ie FUJIFILM Corporation bernimmt keine Haftung f r Verletzungen von Copyright oder anderen Rechten Dritter die aus der Verwendung jeglicher Informationen dieser Anleitung entstehen k nnen Die FUJIFILM Corporation bernimmt keine Haftung f r Ger te Ausf lle oder Besch digungen die durch Installation Standortver nderung Ver nderungen Wartung oder Instandsetzung entstehen sofern diese nicht durch die FUJIFILM Corporation oder von der FUJIFILM Corporation genannte Auftragnehmer durchgef hrt wurden Die FUJIFILM Corporation bernimmt keine Haftung f r Ausf lle und Besch digungen die durch Verwendung von Produkten von Drittherstellern verursacht wurden Die FUJIFILM Corporation bernimmt keine Haftung f r Ger te Ausf lle und Besch digungen die durch Ver nderungen Wartung Instandsetzung usw unter Verwendung von Ersatzteilen verursacht wurden die nicht von der FUJIFILM Corporation angegebene Originalteile sind Die FUJIFILM Corporation bernimmt keine Haftung f r Ger te Ausf lle und Besch digungen die auf die Nichtbeachtung der in dieser Anleitung beschriebenen Vorsichtsma regeln und Betriebsverfahren zur ckzuf hren sind Die FUJIFILM Corporation bernimmt keine Haftung f r Ger te Ausf lle und Besch digungen die auf den Betrieb des Ger ts unter Bedingungen zur ckzuf hren ist die den in dieser Anleitung beschriebenen Betriebsanforderungen Stromversorgung Betriebsumgebung etc nicht entsprechen
39. ing QuickGene Mini80 disconnect the plug from the outlet first If the power cable is damaged this may cause a device failure fire injury or electric shock Caution Do not use QuickGene Mini80 in an unstable place such as a slanted surface or a place subject to vibrations It may cause injury or device failure Do not use QuickGene Mini80 in direct sunlight or close to a heating device It may shorten the life of QuickGene Mini80 or cause a trouble For correct and safe use of QuickGene Mini80 use it in a location that meets the following conditions e The space shown in the following figure must be allocated Top 30 cm Rear 5 cm Left he Right 20 cm A location where power can be provided This is a Class Il device Be sure to use the attached AC adapter The El mark is the mark of the Class II device A location where the temperature is 15 to 30 C and humidity is 30 to 80 RH non condensing A location that is flat and stable with no vibration A location away from direct sunlight Block the sunlight by closing curtains or blinds as necessary A location which is well ventilated and not dusty A location where the temperature does not go up and down suddenly Warming a cold room suddenly or moving QuickGene Mini80 from a room with low temperature to a warm room may cause condensation inside the device resulting in abnormal extraction A location where t
40. ith a soft paper tissue 15 Set the tube holder and cartridge holder into the QuickGene Mini80 again Important After setting it check the following The cartridge holder is placed against the stopper inside the QuickGene Mini80 The WASH label is placed under the tube holder cannot be seen Tube Holder WASH Label Cartridge Holder Note Make sure that the WASH label is hidden below the tube holder before starting pressure application 16 Repeat Steps 3 and 5 Rotate up Tenet 17 Return the rotary switch to the original position to raise the pressure heads Pressure Head Cartridge Important Make sure that no liquid remains in the Cartridges Note Do not pull out the tube holder and cartridge holder while the pressure heads are lowered HOA sn le 897N100125B QuickGene Mini80 User s Manual 2007 6 23 Rotary Switch 2 Operation Cartridge Holder Tube Holder Notes on Incubation mAfter Extraction Operation Release Lever Cartridge 24 897N100125B QuickGene Mini80 User s Manual 2007 6 18 Pull out the tube holder and cartridge holder 1 When the extraction process is followed by incubation leave the QuickGene Mini80 at the room temperature with the tube holder and cartridge holder set Remove the cartridge holder from the tube holder and then dispose of the Cartridges When sliding the release lever to
41. kGene Mini80 1 Set the Waste Tubes and Collection Tubes into the tube holder and then insert the cartridge holder into the Wash Position W of the tube holder 2 Set the Cartridges into the cartridge holder 1 3 Apply lysate to the Cartridges and then set the cartridge holder and tube holder in QuickGene Mini80 4 Rotate the rotary switch toward the front side to apply air pressure to the Cartridges 5 Make sure that Iysate is discharged completely and then return the rotary switch to the original position to terminate pressure application 6 Pull out the cartridge holder and tube holder 7 Apply Wash Buffer to the Cartridges and then set the cartridge holder and tube holder in QuickGene Mini80 8 Rotate the rotary switch toward the front side to apply air pressure to the Cartridges 9 Make sure that Wash Buffer is discharged completely and then return the rotary switch to the original position to terminate pressure application 10 Repeat Steps 6 to 9 depending on the protocol of the relevant Isolation Kits 11 Pull out the cartridge holder and tube holder and then insert the cartridge holder into the Elution Position E of the tube holder 12 Apply Elution Buffer to the Cartridges and then set the cartridge holder and tube holder in QuickGene Mini80 13 Rotate the rotary switch toward the front side to apply air pressure to the Cartridges 14 Make sure that Elution Buffer is discharged completely and then
42. le that meets your country s standard The specification of the power cable for QuickGene Mini80 is as follows 100 120V area Power Voltage 100 to 120 V Requirements for the plug connector AC 125V3A Requirements for the cable SUT 2 x 18 AWG 60 C 200 240V area Power Voltage 200 to 240 V Requirements for the plug connector AC 250 V3 A Requirements for the cable CENELEC 2 x 0 75 mm 70 C 897N100125B QuickGene Mini80 User s Manual 2007 6 3 1 Setting Up 1 3 Remove the Packing Materials After unpacking QuickGene Mini80 from the box proceed as follows 1 Remove the tape that holds the rotary switch and cushion and then remove the cushion Important Keep the shipping box and the removed cushion They will be needed again when transporting QuickGene Mini80 Note The cushion is for protecting pressure heads from being damaged Rotary Switch Cushion Cushion 2 Place QuickGene Mini80 upside down and then make sure that the pressure heads are fully inserted Note Place QuickGene Mini80 on a soft cloth etc to prevent damage to the body Pressure Heads 4 897N100125B QuickGene Mini80 User s Manual 2007 6 1 Setting Up 1 4 Overall View This section explains the name and function of each part in QuickGene Mini80 amp Front and side view Pressure Head Lamp LED Rotary Switch Handle Stopper Cartridge Holder Tray Tube Holder HRear view DRRR 0 REDUCE
43. limentation environnement de l installation etc d crites dans ce manuel e FUJIFILM Corporation ne peut tre tenu pour responsable d une d faillance ou de dommages de l appareil dus des catastrophes naturelles telles qu un incendie un tremblement de terre des inondations ou des clairs e Aucune partie de ce manuel ne peut tre reproduite sans l autorisation de FUJIFILM Corporation m Marques Les noms de compagnies et de produits sont des marques ou des marques d pos es de leurs propri taires respectifs Tous droits r serv s Copyright FUJIFILM Corporation 2006 2007 897N100125B QuickGene Mini80 User s Manual 2007 6 V vi Conventions Ce manuel contient des informations importantes concernant l utilisation en toute s curit du QuickGene Mini80 Lisez attentivement ces informations avant d utiliser et de faire fonctionner le QuickGene Mini80 Les significations des symboles de s curit utilis s sont les suivantes _ WARNING signale des conditions de dangerosit qui si elles n taient WARNING ggg x z pas vit es pourraient entra ner la mort ou des blessures graves A BIOHAZARD BIOHAZARD indique l exposition un agent biologique ou une situation qui peut tre infectieuse ou nocive A CAUTION CAUTION signale un danger possible qui s il n tait pas vit pourrait entrainer des blessures corporelles mineures ou l g res CAUTION pourrait aussi indiquer un
44. llers zu ver ndern Dadurch k nnte es zu einem Brand oder Stromschlag kommen Legen Sie keine Gegenst nde auf QuickGene Mini80 ab und lassen Sie keine Gegenst nde darauf fallen Setzen Sie es au erdem keinen St en oder hnlichen Ersch tterungen aus da es dadurch zu einem Ausfall oder zu einer Fehlfunktion von QuickGene Mini80 kommen k nnte e Wenn im Ger t irgendwelche Fl ssigkeiten zur ckbleiben wischen Sie es mit einem weichen Papiertuch aus Andernfalls kann es zu einer Besch digung von QuickGene Mini80 kommen Tragen Sie beim Auswechseln der Druckmessk pfe stets Handschuhe Falls Ethanol in das Ger t versch ttet wird ziehen Sie sofort das Netzkabel ab Reparaturarbeiten an QuickGene Mini80 sollten nur von Firmen ausgef hrt werden die von der FUJIFILM Corporation ausdr cklich daf r autorisiert sind Dabei sollten nur Original Ersatzteile von der FUJIFILM Corporation verwendet werden e Wenn das Ger t anders als auf die vom Hersteller vorgesehene Weise verwendet wird k nnen die Sicherheitseigenschaften des Ger ts beeintr chtigt werden 897N100125B QuickGene Mini80 User s Manual 2007 6 xix ber Isolations Kits Nachstehend als Kit Kits bezeichnet N here Informationen zum Umgang mit den Kits finden Sie im CAUTION entsprechenden Handbuch e Der Umgang mit den Reagenzien in den Kits sollte unter Befolgung der Sicherheitshinweise und Vorsichtsma regeln des jeweiligen Kits erfolgen e Die
45. n in this manual e Contents of this manual are not to be reproduced without permission from the FUJIFILM Corporation e Manuals with missing or wrongly collated pages will be replaced free of charge FUJIFILM FUJIFILM Corporation FUJIFILM Corporation Life Science Products Division MIDTOWN WEST 7 3 AKASAKA 9 CHOME MINATO KU TOKYO 107 0052 JAPAN Tel 81 3 6271 2158 Fax 81 3 6271 3136 E mail sginfo fujifilm co jp http lifescience fujifilm com Printed on Recycled Paper
46. protection lorsque vous proc dez au nettoyage propos des chantillons AN BIOHAZARD e Portez toujours au minimum des gants appropri s un masque et des lunettes de protection lorsque vous manipulez des chantillons infectieux xvi 897N100125B QuickGene Mini80 User s Manual 2007 6 Limitations d utilisation du produit e FUJIFILM Corporation n assume aucune responsabilit de r sultats quelle que soit application e Il est de la responsabilit de l utilisateur de valider les performances des Kits d isolation et QuickGene Mini80 e QuickGene Mini80 est un appareil uniquement destin a la recherche et l tude Lors de l utilisation du QuickGene Mini80 lisez d abord ce manuel enti rement et respectez les r glements de gestion de risque de votre lieu de travail laboratoire 897N100125B QuickGene Mini80 User s Manual 2007 6 xvii Sicherheitsinformationen In diesem Kapitel sind die Sicherheitsma regeln beschrieben Um Verletzungen zu vermeiden befolgen Sie bitte die Bedienungsrichtlinien f r QuickGene Mini80 auf den folgenden Seiten Informationen zum A Zeichen Pr fen Sie alle Beschreibungen die mit dem Zeichen versehen sind Informationen zum Ger t ee xviii A WARNING e Bei Missachtung der folgenden Warnhinweise besteht Brand und Stromschlaggefahr Verwenden Sie das beiliegende nur f r amerikanische und kanadische Modelle geeignete Netzkabel nicht au erhalb der USA un
47. rlassende Vorgehensweisen Note Note kennzeichnet Hinweise Vorgehensweisen die befolgt werden sollten und zus tzliche Informationen zur Bedienung 897N100125B QuickGene Mini80 User s Manual 2007 6 Safety Information This chapter describes safety precautions For your safety and that of others follow the guidelines provided in the following pages concerning the use of QuickGene Mini80 About A Mark Check all descriptions with the mark attached About Instrument A WARNING Ignoring the following notations may lead to fire or electric shock A CAUTION 897N100125B QuickGene Mini80 User s Manual 2007 6 In countries other than US and Canada do not use the power cable attached for the US and Canadian models In countries other than US and Canada use a power cable that meets your country s standard or contact your local distributor Use the AC adapter provided Do not use the power cable attached for the US and Canadian models for other products Do not use QuickGene Mini80 with voltage other than the voltage specified on the device Do not overload the power outlet with too many devices Do not use QuickGene Mini80 with a damaged power plug or a loose socket If there is dust on the prongs of the power plug or on the plug socket remove it with a dry cloth When you disconnect the plug from the outlet be sure to hold the power plug itself Do not pull the power cable For maint
48. romptly How to clean replace the pressure heads is described below A Caution When replacing the pressure heads wear gloves 1 Turn off the power Disconnect the plug of the power cable from the outlet 2 Pull out the tube holder and cartridge holder Tube Holder 28 897N100125B QuickGene Mini80 User s Manual 2007 6 3 Maintenance 3 Place the QuickGene Mini80 upside down and then rotate the rotary switch to eject the pressure heads Note Before placing the QuickGene Mini80 upside down spread a cloth etc on the table to avoid damaging the body Rotary Switch Cleaning 4 While holding the QuickGene Mini80 by one hand clean or replace the pressure heads Wipe dirt off the pressure heads with a soft paper tissue soaked with ethanol If the pressure heads are heavily soiled or any abnormal conditions damage deformation 3 hardening etc are found replace them with new ones Note Set the pressure heads in the direction shown in the figure and push them completely into the body Replacement _ Le LS an lt fr Head 5 Rotate the rotary switch to retract the pressure heads Pressure Heads Rotary Switch 897N100125B QuickGene Mini80 User s Manual 2007 6 29 3 Maintenance 30 897N100125B QuickGene Mini80 User s Manual 2007 6 4 Troubleshooting 4 Troubleshooting This chapter explains the procedures that should be taken when you suspect device f
49. rque A Mark fournies A propos des instruments A WARNING A CAUTION e Si les recommandations suivantes ne sont pas prises en compte un incendie ou une d charge lectrique peut se produire En dehors des Etats Unis et du Canada n utilisez pas le cable d alimentation fourni avec les mod les vendus aux Etats Unis et au Canada En dehors des Etats Unis et du Canada utilisez un cordon d alimentation conforme aux r glementations de votre pays ou contactez votre revendeur local Utilisez l adaptateur secteur fourni N utilisez pas sur d autres produits le c ble d alimentation fourni avec les mod les vendus aux Etats Unis et au Canada N utilisez pas le QuickGene Mini80 avec un voltage diff rent de celui sp cifi sur l appareil Ne branchez pas trop d appareils sur la prise lectrique N utilisez pas le QuickGene Mini80 si la fiche est endommag e ou si la prise n est pas solidement fix e Si de la poussi re recouvre les broches de la prise ou de la fiche retirez la l aide d un chiffon sec Lorsque vous retirez la fiche de la prise faites le en tenant fermement la prise Ne tirez pas le c ble Pour tout entretien d branchez la fiche de la prise murale Ne touchez pas la fiche lorsque le tonnerre gronde Ne versez aucun liquide sur le QuickGene Mini80 Ne placez aucun objet contenant du liquide sur le QuickGene Mini80 Vous risquez en le faisant de provoquer une panne
50. s Note Do not pull out the tube holder or cartridge holder while the pressure heads are lowered Pull out the tube holder and cartridge holder 897N100125B QuickGene Mini80 User s Manual 2007 6 Tube Holder ESS 2 Operation lt Wash gt 8 Apply Wash Buffer to each Cartridge Use a pipette to apply Wash Buffer to the Cartridges In this operation take care not to touch the filter in the Cartridge the Cartridge itself or the cartridge holder Note If any Wash Buffer is left on the rim of a Cartridge or on the cartridge holder wipe it off with a soft paper tissue 9 Set the tube holder and cartridge holder into the QuickGene Mini80 again Important After setting it check the following The cartridge holder is placed against the stopper inside the QuickGene Mini80 The WASH label can be seen WASH Label Cartridge Holder 10 Repeat the wash process the necessary number of times according to the protocols Note e Refer to Steps 3 to 8 The waste fluid scale indicates the number of wash processes L Pressure application of lysate completed W1 First wash process completed W2 Second wash process completed W3 E Third wash process completed When the wash processes are completed move the cartridge holder to the Elution Position E to perform the elution process 897N100125B QuickGene Mini80
51. sumes no liability for device failure or damage due to installation relocation remodeling maintenance and repairs performed by parties other than the FUJIFILM Corporation or agencies specified by the FUJIFILM Corporation e The FUJIFILM Corporation assumes no liability for failure or damage due to use of third party products e The FUJIFILM Corporation assumes no liability for device failure or damage due to remodeling maintenance repairs etc using repair parts other than genuine parts specified by the FUJIFILM Corporation e The FUJIFILM Corporation assumes no liability for device failure or damage due to failure to observe the precautions and operation procedures described in this manual e The FUJIFILM Corporation assumes no liability for device failure or damage due to using the device in an environment that does not meet the operational requirements power installation environment etc described in this manual e The FUJIFILM Corporation assumes no liability for device failure or damage due to natural disasters such as fire earthquakes floods or lightning e No part of the contents of this manual may be reproduced without permission from the FUJIFILM Corporation m Trademarks Company names and product names are trademarks or registered trademarks of their respective owners All Rights Reserved Copyright FUJIFILM Corporation 2006 2007 897N100125B QuickGene Mini80 User s Manual 2007 6 Notations This manual con
52. tains important information regarding the safe use of QuickGene Mini80 Read this information carefully before using QuickGene Mini80 and start operation The meanings of safety precaution marks are as follows A WARNING WARNING indicates a dangerous condition that may lead to death or serious injury AN BIOHAZARD BIOHAZARD indicates exposure to a biological agent or condition that may be infectious or harmful A CAUTION CAUTION indicates the possibility of danger that may lead to minor or 3 moderate personal injury CAUTION may also indicate an accident of property damage Important Important shows the important notes for usage as well as prohibited actions Note Note indicates the notes procedures that should be obeyed and supplementary information for use 897N100125B QuickGene Mini80 User s Manual 2007 6 QuickGene Mini80 QuickGene Mini80 QuickGene Mini80 DNA RNA QuickGene Mini80
53. y objects near the outlet so that the plug of the power cable can Plug be disconnected immediately in case of emergency 14 897N100125B QuickGene Mini80 User s Manual 2007 6 2 Operation 2 2 Preparation The following preparations are required for extraction operation ltems to be prepared Gloves Mask Safety Goggles Collection Tubes CT 1 2 Waste Tubes WT 1 Cartridges CA 1 Buffer Wash Buffer Elution Buffer 1 2 Lysate Samples For the preparation of samples refer to the handbook of each kit 1 Provided with the Isolation Kits Tube adapters are required when using Collection Tubes provided with the Isolation Kits When using Collection Tubes included in the Isolation Kits P 17 2 Commercially available 1 5 ml micro tubes can be used in place of Collection Tubes MPreparation of lysate Prepare lysate Make lysate using the specified kit for each target sample For details refer to the handbook of the Isolation Kits 897N100125B QuickGene Mini80 User s Manual 2007 6 15 2 Operation 2 3 Extraction This section explains extraction procedure including before and after extraction operations Be sure to perform extraction in the following order mBefore Extraction Operation Lamp LED ON 1 Turn on the power by connecting the power cable and then make sure the lamp LED lights 2 Pull out the tube holder and cartridge holder Tube Holder 3 Remove t

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