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Liquid GC autosampler

1. this value Pressing Y enters the following screen HT300A UK B doc 5 9 HT300A User manual Pre Wash Solvent O amp Post Wash Sol vent Screen 36 The first line selects the pre washing solvent Field may have the values A B C or D corresponding to the two bottles positioned on the left and right side of the waste bottle see figure 4 The second line selects the solvent used for the post washing operations As with the prewash field may have the values A B C or D corresponding to the two bottles positioned on the left and right side of the waste bottle see paragraph 2 1 3 If you DON T want to make further changes to the step just modified or to other steps in the sequence it is possible to EXIT with SAVING the modifications by following this procedure Go to screen 32 press 00 or press EXIT e Press SEQ The unit will go to the Run Time Menu NOTE Every step modification in a sequence will have immediate validity and will be automatically saved It is not possible to exit from this mode without saving the modifications If you wish to make further modifications to this step or to other steps in the sequence it is possible by Enter screen 37 press key from screen 33 34 35 36 Sequence Saving NXT INS DEL END Screen 37 Field O displays the number of the step that is currently being modified In this screen press and C to
2. Connect cables see paragraph 3 3 3 Electrical Connections I nstall the Tray with the autosampler switched off see paragraph 3 3 4 Tray installation with the autosampler switched OFF Switch on the autosampler see paragraph 3 3 5 Start Up Place waste and solvent bottles see paragraph 6 4 Installation and removal of solvent and waste bottles Place one sample vial in position Ol See paragraph 2 1 5 Vials location Set correct parameters for Vial Size and Vial Depth in the menu Vial Syringe Set see paragraph 4 6 Install syringe see paragraph 4 7 1 First installation Align the autosampler on the GC Injector s see procedure paragraph s 4 5 1 Alignment for a single injector 4 5 2 Alignment for two injectors Conclude the installation switching off the autosampler see paragraph 3 4 or leave the set up mode see paragraph 4 2 HT300A UK B doc 3 3 HT300A User manual 3 3 1 Voltage modification A Ensure that autosampler is not connected to the main power On the back of the autosampler there is the connection panel as reported on Figure 3 The voltage is written in white on a red background on the top left hand side Autosampler is normally set on 230V It is easily possible to change it to work with either 115V or 230V To change voltage proceed as follow Bern E T KC 1 V Figure 8A Figure 8B 1 Open fuse compartment with a screwdriver
3. HT300A User manual Sequence Mode Sequence mode is the Menu page where to edit a sample list or a sample batch To reach the sequence mode press the key on the control panel This mode can be used to display change or edit up to 16 sequences The sequence is a program a list of instructions that defines an automatic injection cycle Automatic Run The program is divided in 16 steps identified by a number from 1 to 16 A step defines the geometric parameters involved in the injection cycle i e Vial position and the method Each step can works alone as a program see paragraph 7 3 Automatic Run or can be used with other steps see Screen 11 Creating a step Press on the control panel to enter the sequence mode The key will light and the following screen will be displayed Sequence Step 4 Tray Configur 7Y Screen 32 The first row show the number of the selected step Press 3 and C to change this field between 1 to 16 The second row defines the type of tray to use This may to be different from the one currently installed Field can have the following values 110H 55x2 positions Highthroughput tray for 2 2 5 ml vials NOTE It is always possible to come back to screen 32 from any other screen of the sequence mode by pressing EXIT Pressing Y enters the following screen HT300A_UK_B doc 5 8 HT300A User manual A Tray Configur O lt Method Number V Screen 33 The first lin
4. Example The method has been set to use a 40 position but a 110 position is installed e High Throughput Injection Mode has been chosen see screen 4 but the tray type doesnt support this mode E g the tray is defined as 110 instead of 110H Solutions e Press EXIT and install a different type of tray EXIT e Press and change the set up information defined in sequence step indicated see paragraph 5 4 e Press EXIT set up the sequence in a way to avoid steps requiring a tray different from that installed e Select a type of tray able to support the High Throughput Mode see paragraph 5 4 see paragraph 8 1 Pre sol v vol max Met O Stp NXT Screen E21 Cause HT300A UK B doc 9 4 L 7 Li HT300A User manual e The set value for Pre Volume volume of solvent used for pre washing operations of the syringe is higher than the nominal volume set for the syringe Solution e Press EXIT and replace the syringe with one syringe with a higher capacity e Press EXIT and change the set volume for the syringe in Set up if this is wrong e Press EXIT and lower the pre Volume in the method Post solv vol gt max Met O Stp NXT Screen E22 Cause e Value set for Post Volume volume of solvent used for post washing operations of the syringe is higher than nominal volume set for the syringe Solution Press EXIT and change set volume for post washing Press
5. WASTE Waste absence Put in then press START Screen E05 Vial size msmatch Check vial on tray then START Screen E06 HT300A UK B doc 14 7 HT300A User manual WARNI NG WasteFail START gt Continue EXIT abort Screen E10 WARNI NG Vial absence press EXIT then fixup Screen E11 WARNI NG Check Touch Sensor Screen E12 WARNI NG Obstacle found press EXIT then remove Screen E13 Tray range mismatch Met Stp NXT Screen E20 Pre solv vol max Met Stp NXT Screen E21 Post solv vol max Met O Stp NXT Screen E22 Filling volume gt max Met Stp NXT Screen E23 Sample volume gt max Met O Stp NXT Screen E24 Sample Air vol max Met O Stp NXT Screen E25 SMP IS SLV vol max Met O Stp NXT Screen E26 Wrong IS SLV Range Met Stp NXT Screen E27 HT300A_UK_B doc 14 8 HT300A User manual SCREENS AND FI GURES INDEX Figure 0 DTS ankeren 5 6 ODA Nn 2 1 ENEE EE 5 4 DUR A e Na dont 2 1 EE ee 5 4 EE EE 2 2 BOR eene 5 5 DEE Ed 2 3 Eh NEE E ARNE 5 7 tee EERE 2 3 EE 5 8 EE EN NN 2 4 II ORN EN aa Na 5 9 OS Beert en 2 4 c NE MACRO NR APER 5 9 BE EE 2 5 OEE ENNE 5 9 DB NN hermen De cats a 2 5 TEN CEN IO MK 5 10 Ober usd DN mda eas 2 5 ER ONU ANNA 5 10 A rd etn den 2 6 e e 4 1 OG KAGAN SE NAN ete 2 6 GENDEREN 4 3 e 2 7 EE 4 3 Ee 27 ns aa de 4 4 OCE 2 7 EE 4 4 OLD ten ante ed 2 7 TRC AGE AERO WKE 4 5 GER 2 8 EE EE 4 5 E MA ANAN KA TAN
6. EXIT and replace the syringe with one having a larger capacity Press EXIT and change set volume for the syringe in Set up if this is wrong with reference to nominal volume of the syringe Filling volume gt max Met Stp NXT Screen E23 Cause e Set value for Fill Volume volume of sample used for washing with sample procedure is higher than the nominal volume set for the syringe Solution Press EXIT and reduce the Fill Volume value Press EXIT and replace the syringe with one having a higher capacity Press EXIT and change the volume set for the syringe in Set up if this is wrong with reference to the nominal volume of the syringe Sample volume gt max Met O Stp NXT Screen E24 Cause HT300A UK B doc 9 5 HT300A User manual e The value set for Sample Volume volume of sample used for the analysis is higher than nominal volume set for the syringe Solution Press and reduce the value set for Sample Volume e Press ET Lane replace the syringe with one having a higher capacity e Press and change set volume for the syringe in Set up if this is wrong with reference to nominal volume of the syringe Sample Air vol max Met O Stp NXT Screen E25 Cause e The sum of the value set for Sample Volume volume of sample used for the analysis plus the value set for Air Volume volume of air used is higher than nominal volume set for the syringe Solution Press a
7. HT300A Usr manual T 300A Liquid GC autosampler HT300A_UK_B doc HT300A Usr manual This manual contains information about the HT300 GC autosampler for liquid samples The information contained in this document is subject to change without notice This document is for information only the manufacturer accepts no liability for errors contained herein or for incidental or consequential damages with the furnishing performance or use of this material Hamilton is a trademark of Hamilton Company Unless otherwise specified references to names or parts is purely casual and has the purpose of illustrating the product The contents of this publication may not be reproduced in any form or by any means including electronic storage and retrieval or translation into a foreign language without prior agreement and written consent from the copyright owner All rights reserved Version B 08 09 2003 HT300A UK B doc i SUMMARY 1 1 1 3 1 4 1 6 2 1 1 2 1 2 2 1 3 2 1 4 2 1 5 2 1 6 2 3 2 3 1 2 3 2 2 3 3 2 3 4 2 3 5 3 1 3 2 1 3 2 2 5 2 1 5 2 2 b 3 sar HT300A Usr manual INTRODUCTION Manual Structure Applicable rules Symbols table General specifications creer pr rper pe PS CU Vers te aan egin eet sosuse ease ie ee ap a vp ue nennen Versions sasae senasa aga nenika Technical Specifications s Ge EQUIPEMENT DESC
8. Screen E01 Field O displays the type of tray previously installed 110 110 position rotating tray for 2 and 2 5 ml vials 40 40 positions rotating tray for 10ml vials To install the tray when the autosampler is ON follow these instructions HT300A_UK_B doc 6 3 6 3 E 2 10 HT300A User manual Hold firmly the middle part of the tray Place the tray on the bracket as shown in the Figures below gre Figure 9C Figure 9D Before Installation After installation bi Gently rotate the tray clockwise until it reaches the insertion point As soon as the Autosampler detects the tray it will display following screen TRAY O installed Press START to continue Screen E02 Field displays XXX Press Wait until the autosampler detects the tray this operation can take a few minutes During this operation the following message will be displayed TRAY DENTI FYNG PLEASE WAIT Screen 02 If the tray typed is Not detected an error message will be displayed refer to chapter 9 Troubleshooting Remove the tray and restart from point 1 If a 40 or 110 position tray is detected from the Autosampler screen E02 see above will show 40 or 110 in field If the tray type or the vial size are changed it s necessary to change the set up parameters see paragraphs 4 2 and 4 6 Removing a Tray To remove a tray 1 2 Ensure that the turret is not over the tray if not move the
9. To use 00 please go to paragraph 4 3 Manual Operations 4 5 2 Alignment for two injectors From screen 57 it goes to screen 61 Needle in SPEED lt Front Inj Align d Screen 61 Field O sets up the descent speed of the needle into the first injector The speed is indicated with a value between 0 and 8 Gee table 2A paragraph 4 4 2 of this chapter Press then to enter the alignment mode on to the front injection port Screen 63 will be displayed Pressing EXIT will revert to the preceding screen screen 61 saving the modification made Front Inj Alignment HT300A UK B doc 4 7 HT300A User manual TURRET lt gt v NEEDLE Screen 63 The tower will turn reaching the position previously stored in memory Using the 2 and C keys to align it on the injector When the tower is in the correct position use the 00 keys to lower the syringe onto the injector This point is defined as touch point As soon as the needle touches the injector screen 63A appears Front Inj Alignment TURRET STO NEEDLE Screen 63A Press EXIT to exit alignment mode without saving Press ENTER to confirm In this case screen 64 will be displayed Front Inj Alignment STO lt mm DEPTH Screen 64 Move the syringe holder using the keys and O in order to insert the needle into the injector at the desired depth Field displays the depth reached by the needle This value is variable from 0 to 99
10. following positions The column is aligned in the central position over the waste vial The column is aligned on the sample tray this will be on the left or the right according to the sampler configuration If the system is configured with a single injector field can have also the following value Injec The column is aligned on the injector If the system is configured with two injectors field will have also the following values HT300A_UK_B doc 6 1 HT300A User manual Front The column is positioned on the first injector Rear The column is positioned on the second injector Pressing goes to the following screen INJ SYNCRO O INJ MODE o v Screen 04 The first row there is the synchronism type to use that is the timing of the signal emitted upon injection SMP Instant Field may have the following values Type of Emission SMP Instant Start I njection synchronism Output to analyser signal from the analyser At the beginning of the At the reception of the signal Normal syringe plunger movement GC Ready At the reception of the signal GC Ready it starts the At the beginning of the collecting then it waits for an syringe plunger movement external synchronism SYNC IN to continue with the injection Trigger In the moment it touches At the reception of the signal the injector GC Ready At the end of the Delayed movement of the syringe plunger A start At the re
11. modified using or and can be set to 2 2 5 or 10 last value can only be selected if there is a 40 position tray installed Field displays the depth the needle penetrates into the sample vial to remove the sample This field may have values between Omm and a value relating to the dimensions of the vial installed see below Press 3 and C to increase 9 or to decrease this value in steps of 1 mm Vial Size Max Vial Depth Table 3 Press the EXIT key to go back to Screen 50 saving changes Pressing Y enters the following screen Syringe Size O ul Syringe Install E Screen 68 Field O displays the syringe nominal value indicated in microliters Press 2 and C to change this value it can be 10 25 50 100 250 or 500 Press twice to enter the following screen Syringe Instal gt Touch amp Plunger Zero gt Screen 69 HT300A_UK_B doc 4 10 HT300A User manual Press and to start the syringe installation mode see paragraph 4 7 Syringe Installation and to set e The ZERO POINT for syringe plunger e The TOUCH POINT over the waste bottle e The TOUCH POINT over the vial 1 of the tray The following screen will appear ZERO PLUNGER WAIT RUNNING Screen 70 The plunger reaches the calibration position then displays the following screen AUTOMATI C START MANUAL STO Screen 71 Press the START key to start the automatic procedures these do not require a
12. 1 HT300A User manual TRAY O not Identified Waiting Tray Screen E04 Causes e The type of the tray installed is not recognised e The tray is not installed correctly e The tray identification procedure has been interrupted Solution Press EXIT Remove the installed tray and repeat the tray installation procedure It is necessary to wait for the autosampler to complete the identification procedure Do not press EXIT during this procedure 9 4 Obstacles WARNI NG Obstacle found press EXIT then remove Screen E13 Causes The autosampler has found an obstacle on the waste The autosampler has found an obstacle on a vial e The autosampler has found an obstacle on an injector Solution Remove the obstacle Then press EXIT to re start the running operation 9 5 Set up errors 9 5 1 Lack of waste bottle WASTE Waste absence Put in then press START Screen E05 Causes e The waste bottle is missing Solutions Insert a waste bottle into its seat e Press START 9 5 2 Vial with wrong dimensions HT300A UK B doc 9 2 HT300A User manual Vial size mismatch Check vial on tray then START Screen E06 Causes e Vial dimension set doesn t coincide with the real dimension of the vial such as having a 2ml vial on the tray but the system setup for 2 5ml Solutions Replace the vial and press START e Press EXIT Modify the set up of vial dimensions in set up 9 6 A
13. 10 to 500 ul 1 ul stepwise from 1 to 100 ul s from O to 15s from 1 to 100 ul s from O to 99 s 0 1 ul stepwise 0 1 ul stepwise one or two air gaps software to programming HT200A autosampler by PC 1 5 Chapter HT300A User manual 2 EQUIPEMENT DESCRI PTI ON 2 1 Parts Definition Back gt A Si 4 2 6 AO Ba 5 A Front 3 Figure 1A AUTOSAMPLER TOP VIEW Pocket Version HT300A_UK_B doc Back A A A A 1 2 6 AG 94A B E Front 3 Figure 1B AUTOSAMPLER TOP VIEW Standard Version TOWER where is located the syringe I NJ ECTI ON AREA area where it is possible to make an injection Fixing holes to secure the sampler to the GC Bracket 1 TRAY vial plate 110 40 positions 2 3 CONTROL PANEL 4 POCKET 5 WASTE VI AL 6 A 2 1 HT300A User manual 2 1 1 Control panel The control panel of the unit is laid out as follows Figure 2 KEY BOARD Display 0 Commands are showed on an alphanumeric display VFD Vacuum Florescence Device 20 caracters 2 lines placed in the middle Keys SET enters the Set up mode MET enters the Method mode SEQ enters the Sequence mode STOP operative key START operative key EXIT operative key ENTER operative key O directional key up WO o DU B WN Mm directional key right hand side 10 O directional key down 11 C directional key left hand side A ST BY status indicator B RUN status indicato
14. 9 Troubleshooting For an accurate first installation don t release the needle guide regulator see paragraph 4 7 1 First installation When shipped the autosampler has the safety lock as shown in Figure 8C HT300A_UK_B doc 3 5 10 11 12 13 HT300A User manual Figure 8C Figure 8D SHIPPING POSITION OPERATING POSITION 1 Needle Guide regulator 2 Safety lock 3 Needle Guide With the Allen key supplied with the autosampler release the LEFT block labelled 2 in Figure 8C The RIGHT block needle guide regulator is about 50mm above the left one when the unit is shipped see Figure 8D 1 Let the syringe mechanism down so that it rests on the right block and tighten the left block when done the left block should be slightly above the right one Setting it correctly allows the needle guide to be lifted by hand see Figure 8C 3 to the position shown in Figure 8C Gently rotate the tower manually moving it over the injector Move down the Needle Guide see figure 7C over the injector pressing down the Syringe location see figure 7C Adjust the sampler position The needle must be centred on to the injector Firmly fix the autosampler by tightening the four screws Move the Needle Guide back up to the home position Rotate the tower back to the original position Close the protection lid HT300A_UK_B doc 3 6 HT300A User manual 3 3 3 Electrical Connections Follow these instr
15. ADVANCED FUNCTIONS 8 1 High Throughput Mode 8 1 1 DESCRIPTION High Throughput Mode will only work on GCs that have two injection ports In this mode two different samples are injected into the two injectors The tray is divided in to two parts the first half is associated to the Front Injector and the second half is associated to the Rear Injector The first half and second half have corresponding positions these are Fixed tray POCKET Version e Vial position 0 is associated with vial position 5 e Vial position 1 is associated with position 6 e Vial position 2 is associated with position 7 e Vial position 3 is associated with position 8 Vial position 4 is associated with position 9 Rotating tray STANDARD Version e Vial positions in sector 0 are associated with vials of 5 e Vial positions in sector 1 are associated with vials of 6 e Vial positions in sector 2 are associated with vials of 7 e Vial positions in sector 3 are associated with vials of 8 e Vial positions in sector 4 are associated with vials of 9 Example Vial 01 sector 0 position 1 is associated the vial 51 sector 5 position 1 Vial 29 sector 2 position 9 is associated the vial 79 sector 7 position 9 8 1 2 Use in a single injection procedure To use this mode of operation is necessary to authorise the use of High Throughput Mode that is in screen 04 to set up as nj Mode the value Hithrou 8 1 3 Using Hithrouput
16. Diagram of the METHOD mode HT300A_UK_B doc 13 4 HT300A User manual 13 4 Runtime mode Display the type of tray identifiedby the TRAY MODEL 110 40 10 autosampler TURRET ON Tray Waste Inject Moves the turret of the sampler over the waste bottle vial tray Injector s INJ SYNCRO Use the amp to switch the Injection synchronisation Premere EXIT per ritornare al menu SYRINGE WASH Wash solvent A D Select the solvent to wash with Wash Repeat 1 15 Select the number of washes Volume uL Volume of solvent for each wash from 0 1 uL to installed syringe nominal volume Press START to run wash cycle Press EXIT to return to menu SINGLE INJECTION Method Number 0 9 Select method Sample Number any Select sample to inject Int Std Solvent any vial Select Internal Standard if used PreWash Solvent A D Solvent to wash syringe before injection Post Wash Solvent A D Solvent to wash syringe after injection Press START to run single injection cycle Press EXIT to return to menu AUTOMATIC RUN First Step Set First step of the sequence to run Press START any time to run Last Step Set Lastt step of the sequence to run automatic injection cycle End Seq Output Yes No Ru nan nj ectio ns seq uence Sends a signal on completion of the whole sequence PowerOn Restart Yes No Continue with run after a power failure Empty Pos Sync Yes No If vial is abse
17. Fil Spduls 1 100 Internal Standard sample used in the Fill Spduls 1 100 IntStd Vol ul 0 500 sample preparation The Air gap Mode defines the sequence batch in the sample preparation The Air Gap is the volume inserted between sample and the IntStd sample as forward defined 1 i Th i i ill be The Fill Vol is the volume Air Gap Mode Post Double A E e re us the syringe will be used in the clean with Air Gap pl 0 500 1 sample cycle and in the i Air Gap Vol IntStd Vol bubble elimination IntStd Vol Air Gap Vol pull up strokes cycle i 1 i The Filing Speed is the Air Gap Vol Sample Vol sampling speed i Sample Vol Air Vol Air va 1 withdrawing under septum Set the number of times the system should be flushed with sample Set the number of pullup strokes to use to remove air bubbles from the syringe Set the volume for each Sample Washes we Pullup Strokes Set the time that the syringe stays in the sample after Visc Dy sec 0 15 the plunger has been raised to allow for viscous In Spd nis 1 100 samples Also set the injection speed when it is set to maximum it will read HIGH Pre Inj Dwl sec 0 99 Post Inj Dwl sec 0 99 Set the wait time before and after the injection with the needle in the injector Press ENTER to Saves the method to memory press ENTER It will Copy Method x save the method you are currently editing X to position Y in memory Flow
18. O Y Screen 57 Pressing 2 and C to change the number of the injectors So field will have the value 1 or 2 This value will be saved at the moment of the modification Pressing enters the following screen If the autosampler is set to work with a single injector it will show screen 58 please consult the sub paragraph 4 5 1 Alignment for a single injector HT300A_UK_B doc 4 5 4 5 1 HT300A User manual If the autosampler is set to work with two injectors it will show screen 61 please consult the sub paragraph 4 5 2 Alignment for two injectors Alignment for a single injector From screen 57 press down to screen 58 Needle in SPEED lt Injector Align gt Screen 58 Field O is the descent speed of the needle into the injector This speed is indicated by a value between 0 and 8 see table 2 paragraph 4 4 1 of this chapter The second line of the screen accessed by O then D is the alignment mode of the autosampler on the injector In this case it will display screen 59 Injectors Alignment TURRET lt gt gt NEEDLE Screen 59 The tower will turn reaching the position previously stored in memory Use 3 and to turn the tower and align it on to the injector When the tower is in the correct position use O0 to move the syringe down onto the injector This point is defined as touch point As soon as the needle touches the injector the following screen will appear Injectors Align
19. PLUNGER WAIT RUNNING Screen 70 AUTOMATI C START MANUAL v STO ai Screen 71 ZERO PLUNGER WAIT RUNNING Screen 72 WASTE TOUCH WAIT RUNNING Screen 73 VIAL 1 TOUCH WAIT RUNNING Screen 74 14 8 Method Mode Method Number O Internal Std v Screen 20 HT300A_UK_B doc 14 5 HT300A User manual Pre Wash O Volume pl v Screen 21 Post Wash O Volume pl Screen 22 Pre Wash Mode O Post Wash Mode 4 Screen 23 Sample Vol ul O Air Vol pl Screen 24 Fill Vol pl O Fill Spd us O Screen 25 Sample Washes Pullup Strokes 7Y Screen 28 Visc Dly sec O Inj Spd pls O Screen 29 Pre inj Dwl sec 4 Post inj Dwl sec lt gt Screen 30 Fill Spd pls O IntStd Vol pl v Screen 26 AirGap Mode O AirGap pl v Screen 27 Press ENTER to save Copy Method Das V Screen 31 HT300A UK B doc 14 6 HT300A User manual 14 9 Sequence Mode Sequence Step lt gt Tray Configur Q y Screen 32 Tray Configur Method Number Screen 33 First Sample O Last Sample Y Screen 34 Int Std Solvent lt Sample Repeat Screen 35 Pre Wash Solvent O Post Wash Solvent Y Screen 36 Sequence Saving NXT INS DEL END Screen 37 14 10 Error Messages TRAY O Removed Waiting Tray Screen E01 TRAY O installed Press START to continue Screen E01 TRAY not Identified Waiting Tray Screen E04
20. SAN 2 8 E EE 4 6 e AE e 2 8 EE AE A AA 4 6 A E 2 8 D MERE rs are 4 6 DE PAM UNAM LADEN EE 3 4 GO ee ee 4 7 E EE SQUE 3 4 EEE 4 8 EE Senn al XC EEN 3 6 GD Na NAK EEN EE AN an 4 9 DEE 3 6 Dn La A 4 8 DA nen eric a eret er ak 3 7 ENEE Soest ae NA A ie 4 8 DEE NE 3 7 GA EA te ce Nh 4 8 Tica ie OR WiC ON biet 6 4 gen anette alae ne 4 9 OOD sasa gka des neret dica ebur 6 4 GA E 4 9 Screen EENEG 4 10 DO 4 2 Bd e dia ed 4 11 IS poda ee 3 8 Ch Mm 4 11 02 3 8 6 4 c ee 4 11 GEET 6 1 30 annen eene 4 12 OA Sa tae Gen are ten 6 2 7 6 EE veel 4 12 ODS EE oa LN 4 12 OG tetten nale eg 6 3 EAR NICK RR D 4 12 UI eee 71 rr EDT NG DEKO ME 4 12 OEE 7 1 BOIS ag A er 6 3 9 1 O9 M 7 2 BOR vh ee i A Ua SLE EE 6 4 LO sess 7 2 1 8 OL ARMEN S AGER eue 9 2 IL o hessssssrsriirirrnrernrerenenenenenene 7 3 7 8 EE 9 2 12 eese 7 4 SEET 9 3 13 7 4 EE 9 3 a 7 5 AE APEN MENNE 9 3 14 EE 7 5 7 7 7 9 O ended Ne 9 1 DENNEN EN 7 6 EE 9 2 15 hasssssieisieneienenentntntntntininnnnnnneni 7 8 EE EH 9 4 18 1 1 Eltere ege 9 5 19 7 6 7 7 EE e E 9 5 A 5 1 E A NE PRE 9 5 E 5 2 o E AAN 9 6 E 5 2 205 ege he Re AAA 9 6 E 5 2 S CMM MUR NUR TIEN 9 6 240 sise 5 3 ENEN ENEN 9 7 SE ee 5 4 E EEE END 5 5 HT300A_UK_B doc
21. To be in the screen number 50 From this position press SET Manual Operations The Manual Operation function is used to manually move the syringe piston the syringe the tower and the tray Such operations are not used for the normal use of the autosampler but for checking the movement of the respective parts This menu will be automatically entered when e You want to install substitute or remove the syringe e The autosampler detects an error in its movements In this case after an error message has been displayed this screen will appear automatically It will show a screen similar the following PLG NDL TUR TRY TCH AY AY AY AY O Screen 00 Use the 2 and C keys to select one of the four words PLG NDL TUR and TRY Each word correspond to one particular autosampler part e PLG is the syringe piston e NDL is the syringe holder e TUR is the tower HT300A_UK_B doc 4 2 4 4 HT300A User manual e TRY is the tray The selected part will flash The and O keys move the specific part of the autosampler specifically lift or lower the syringe piston lift or lower the syringe holder rotate tower to the right hand side or to the left hand side 4 rotate tray to the right hand side or to the left hand side 6 If psy appears under the moving part it indicates that an error occurred if it disappears after the movement has stopped it
22. can have values 0 1 2 3 4 5 6 7 8 9 and P for 110 vial tray and values 0 1 2 and 3 for 40 vial tray HT300A_UK_B doc 2 6 HT300A User manual 2 1 6 Syringe location The syringe location is on the tower To access to the syringe location remove the protection screw and open the protective lid Figure 7A Figure 7B PROTECTIVE LID DOWN PROTECTIVE LID UP 1 Protective lid 2 Closing screw Syringe location is formed b the following parts Figure 7C Figure 7D SYRINGE INSTALLED SYRINGE NOT INSTALLED HT300A UK B doc 2 3 HT300A User manual Needle guide Intermediate Needle guide Syringe location without syringe 7C with syringe 7D Needle guide regulator Piston locker on 7C 7D 7E off 7F Syringe locker on 7C 7D off 7E 7F Syringe pointer Piston location Safety lock LO JO U BWN HE Figure 7E Figure 7F SYRINGE LOCATION SYRINGE LOCATION The Piston Locker has two different faces to locate different kinds of pistons These faces are indicated as 5 side and B side Figure 7G Figure 7H S SIDE B SIDE HT300A_UK_B doc 2 8 2 2 HT300A User manual Moving Parts There are six moving parts e The rotating tray named TR can rotate about 355 e The tower following identified as Turret and named TU can rotate 160 respect middle position e The needle location following identified as Needle and named ND can move vertically a maximum of 21
23. doubt see Appendix B 22 Raise the syringe lodging NDL to the top 23 Remove the Syringe pointer 24 Check that syringe needle is correctly aligned as per the following illustration HT300A_UK_B doc 4 13 4 7 2 4 7 3 HT300A User manual NO sr NO 25 Press to exit from 00 The unit will display screen 69 26 Run the Vial Syringe Setup procedure see paragraph Touch Plunger Zero see paragraph 4 6 27 Close the Syringe location pulling down the lid and tightening the screw see Figure 7A and 7B 28 Perform the Injection Setup routine see paragraph 4 5 29 When this procedure has been completed to exit from Set up mode screen 50 press the key Removal 1 Check that autosampler is in standby status 2 Ensure the tower in the stand by position see screen 03 paragraph 6 1 3 Open the Syringe Compartment removing the safety screw with the Allen key supplied Open the protective lid see figure 7A and 7B 4 Enter the SET UP Mode refer to chapter 4 paragraph 4 6 and select Vial Syringe Setup 5 Use O and to enter the syringe installation mode Screen 00 will now appear see paragraph 4 3 for instructions 6 Lower the plunger PLG to
24. is being edited e INS Insert insert a new step Selecting insert will insert a step below the one you are editing e DEL Delete delete a step from the sequence e END Terminate will end the sequence at this step and delete any higher steps Sequence Step NXT INS DEL END Flow Diagram of the SEQUENCE mode HT300A_UK_B doc 13 3 HT300A User manual 13 3 Method mode Defines analytical parameters associated to each sample Method Number 0 9 e Choose the method number to edit Internal standard Yes or No e Enable the use of the internal standard technique Set the number of solvent flushes an the Pre Wash 0 15 volume of the syringe to be performed Volume ul 0 500 before the iniection Set the number of solvent flush and the volume of the syringe to be performed Post Wash 0 15 after the iniection Volume ul 0 500 Set the mode for the pre post weshes Inj washes after every injection Sample washes after every sample if there are multiple Pre Wash Mode Injec Sample Step injections from one vial it will only flush at the Post Wash Mode Injec Sample Step end of the last one Step washes at the end of every step Set the sample volume to inject Sample Vol pl 0 500 Set the air volume inserted into the Air Vol ul 0 500 in h m Se Intstd Y syringe under the sa ple Se Intstd No The Filling Speed is the sampling speed The IntStd Volume is the volume of the Fil Vol ul 0 500
25. max according to dimensional limits and is negative as it is calculated with respect to the touch point Press EXIT to exit alignment mode without saving Press ENTER to confirm AN Warning Take care to ensure the following procedures are met In particular ensure that e The needle is aligned over the centre of the needle guide on the injector e The needle guide fits properly together the injector e The needle has to enters the injector without trouble and penetrates the diaphragm without an excessive bending If it does not check the injector septum type of syringe used and needle tip type when the needle is in the port ensure that it has a little further room to go down about 1 2 millimetres In this position wait for about 20 seconds before pressing to memorise the position The operation is correct if within this period of time screen 00 doesn t appear If screen 00 is displayed the system has found anomalies To use 00 please go to paragraph 4 3 Manual Operations HT300A UK B doc 4 8 HT300A User manual The operator will be brought back to screen 61 From screen 61 Pressing enters the following screen Needle in SPEED lt Rear Inj Align E Screen 62 The screen 62 is similar to screen 61 The only difference is that this is for the second rear injector The instructions for screen 61 should be followed Pressing enters the following screen Rear Inj Alignment TURRET NEE
26. select one of the four options these are NXT INS DEL and END the selected option will flash Press ENTER to execute the option The meaning of the four options is explained in the following table HT300A_UK_B doc 5 10 HT300A User manual This goes to the next step In this case the unit reverts to screen 32 Option INS inserts a method similar to using COPY and PASTE this INS inserts a new identical step next to the displayed one Step 16 NXT of the sequence will be replaced from the new one now inserted This deletes the displayed step All the following steps will be drop DEL one position e g eliminating the step 1 step 16 will be 15 and step 15 will be 14 etc END This erases all the steps above the displayed one HT300A_UK_B doc 5 11 Chapter HT300A User manual 6 UTILITY FUNCTION Run Time Menu After the START UP procedure the autosampler will move the tower to the central position above the oven and the following screen will appear TRAY MODEL O TURRET ON Ka Screen 03 Field O displays the tray type 110 110 positions for 2 and 2 5 ml vials 40 40 position tray for 10 ml vials 0 1 The fixed tray 10 positions for vials 2 and 2 5 ml or the base of the tray is disconnected NOTE The system recognises the tray automatically There is no possibility to manually change it FieldO displays the turret position The turret can be moved using SC to the
27. single injector Visualisation of parameters for two injectors Injection Set up Alignment for a single injector Alignment for two injector mens Vial Syringe Set up Syringe Installation Firstinstallati n eee iste eese A Removal sms Errore Il segnalibro non definito Replacement esse Errore Il segnalibro non definito Replacement of the syringe lodging Errore Il segnalibro non definito PROGRAMMATION ssssssmmnnne Method Mode Creating a method Use of internal standard Exit from method mode HT300A UK B doc i HT300A Usr manual 7 1 1 7 1 2 7 2 7 2 1 7 3 1 14 4 Seguence Mode Creating a step Sc da UTILITY FUNCTION EE Run KEEN E EEN Tray installation with the autosampler ON Remove a Tray asin venen nononono venen enenenenenene zien Installation and removal of solvent and waste bottles Interrupting an injection Priority Derne ADVANCED FUNCTIONS semence High Throughput Mode sisa sen DESCRIPTION sisscssscssssssesessssessseassassesnssosesosvonsvenscsesedssss n i ia Use in a single injection procedure see Use in an automatic injection procedure Troubleshooting Introduction ss Touch Sensor Problems Errore Il segnalibro non defin ito CANT Errore Il segnalibro non definito Obstacles Errore Il segnalibro non definito K
28. the first half Confirm HT300A_UK_B doc 8 2 Chapter 9 1 9 2 9 3 b 3 sa HT300A User manual 9 Troubleshooting I ntroduction The list of the possible errors generated by the autosampler are summarised in this chapter If the solutions indicated do not solve the problem please contact your Supplier or the Technical Assistance Centre Before calling the Technical Assistance Centre please check that e all instructions were carefully followed e all the options listed were tried e all cables are connected correctly Touch Sensor Problems WARNI NG Check Touch Sensor Screen E12 Causes e Injection sensor is not working correctly It is possible that there are mechanical problems Solution Press EXIT The autosampler will enter in the screen 00 from there it is possible to move the needle assembly and tower to try to unblock the system and to solve the problem see chapter 4 e When manual control is finished press EXIT If the problem is solved the sampler will re start the Autorun procedure from the point of interruption Tray removal TRAY O Removed Waiting Tray Screen E01 Causes e The tray has been removed from its seat Solution e Re seat the removed tray or replace with another one It is necessary to wait for the autosampler to complete its identification procedure Do not press EXIT during this procedure see paragraph 6 2 HT300A_UK_B doc 9
29. the lowest possible position 7 Lower about 50 mm of the Syringe location NDL from its highest position 8 Loosen the safety lock block allowing it to slide freely 9 Remove the Piston locker pulling with 2 fingers 10 Remove the syringe locker using the Allen key supplied 11 Return the Syringe location NDL to its highest position 12 Extract the syringe with care Replacement If the syringe to be installed is identical to the one that has just been removed go to point 14 If the syringe to be installed is different from the one that has just been removed follow the instructions as if it was the first syringe installation see paragraph 4 7 1 from point 13 HT300A_UK_B doc 4 14 13 15 16 17 18 19 HT300A User manual Locate the Syringe pointer supplied with the autosampler Install the pointer by resting it on the waste bottle so that it fits the waste cap In this way the Syringe positioning sensor will be between the waste bottle and the needle guide see figure 7D Insert the new syringe Insert the needle into the intermediate neede guide and then into the needle guide insert the syringe body into the holder finally place the plunger into its lodging Replace the Syringe locker tightening the two screws carefully Insert the piston locker ensure it is lodged the correct way with respect to the syringe type refer to Appendix B Check that the installation is correct by ensuring th
30. the menu 2 3 5 STATUS indicators On the command panel on the front of the autosampler there are four lights ST BY RUN A INJ and B INJ B INJ and A INJ indicate respectively that the autosampler is in the phase before Before I NJ ection or after After I NJ ection injection RUN indicates that the autosampler received the commands to perform one of the following actions e SAMPLE WASH e SIGNLE INJECTION e AUTOMATIC RUN ST BY ST and BY indicates that the autosampler has not received any commands and is ready to receive one During an automatic injection cycle light ST BY on together with B INJ or A INJ indicate that the autosampler is waiting a confirmation to sop the procedure HT300A_UK_B doc 21 Chapter 3 1 3 2 3 2 1 HT300A User manual 3 INSTALLATION Unpacking and preliminary operations AN Take care when unpacking the unit Check the integrity of the box to see if it is externally damaged If it is please inform your local dealer and keep the box as evidence When the autosampler has been unpacked check the integrity of the autosampler ensure that all the accessories are provided compare these with the packing list enclosed Ensure that everything listed in the packing list is in the box If not please advise your supplier Autosampler assembly Pocket version e Place the box on a table Open the box then remove the upper foam packing You will find the autosampler and ever
31. the syringe as coded by Hamilton corresponding type of lodging of the syringe and the corresponding position of piston block Syringe Size ul 250 500 Hamilton Type 7001 15 701 702 705 710 725 150 Syringe Lodging N ININININ HE H IN Position piston block B U2 DV DV DV un un By B and S are indicated the two faces of piston block These sides are illustrated in paragraph 2 1 6 figures 7G e 7H For the use of different syringes please check that their specifications are corresponding to that indicated in above table Vial Solvent bottles waste For best results use Sun Sri vials and caps These are available from your HTA supplier HT300A_UK_B doc amp Appendix HT300A User manual 13 Appendix C FLOW DIAGRAMS 13 1 Set up mode HT300A Series Manual Operation PLG NDL TUR TRY TCH Software Rel X Y AY Av AY av H Syringe size Vial Size Vial Depth Needle in injector Speed Depth Actual Parameters For 2 injectors Needle in Front Speed Depth Needle in Rear Speed Depth These parameters are view only not settable Injection Set up Injection Setup Injectors Numbers 1 or 2 Needle in Speed Injector lt p 4 Align i Move the injector head over the M i top of the injection port until it touches and the display reads Injector Alignment 2 TURRET o NEEDLE For 1 inijector 1 Inje
32. turret in another position to move the turret please refer to screen 03 paragraph 6 1 Firmly hold the middle part of the tray HT300A_UK_B doc 6 4 6 4 HT300A User manual 3 Pull up the tray from its holder This procedure can be done with the autosampler on or off If the autosampler is on please take care that it is in stand by status if not wait for the end of run or press STOP to abort it If the tray is removed when the autosampler ON see paragraph 6 2 screen E01 I nstallation and removal of solvent and waste bottles To install remove replace fill up empty out or clean the Solvent vials see Figure 4 and or the waste vial perform the following operations in the given sequence 1 Goto screen 03 see paragraph 6 1 2 Change the option TURRET ON to Tray for easy access to the vials For further information on this operation refer to paragraph Run Time Menu of this chapter Removing the vials To remove the waste bottle or a solvent vial raise and extract it with care Cleaning the vials Remove the vial s e Carefully remove the plastic cap e Empty and wash the glass vial s and the plastic cap s e Refit the cap Installation e Put back the vial in its original place To Fill the Solvent Vial s Remove the vial s from its position and clean it Fill the vial with the solvent HT300A_UK_B doc 6 5 Chapter 7 1 7 1 1 HT300A User manual 7 BASIC FUNCT
33. 0mm e The plunger following identified as Plunger and named PL can move a maximum of 64mm inside the syringe Warning If necessary it is possible to move by hand very carefully each moving part but only when the autosampler is switched off Rapid movements or movements with the autosampler switched on can damage the Autosampler 2 3 2 3 1 Control panel description Typographic Notes For a better understanding each screen will be schematised as follow Screen Normal characters see Line 1 identify static lines italic characters see Line 2 identify dynamic lines that is lines that can scroll The symbols indicate fields that can have different values These can be modified when needed HT300A_UK_B doc 2 9 2 3 2 2 3 3 HT300A User manual The symbols ADOG indicate keys 8 9 10 11 that are located in the front of the autosampler see figure 2 The words and indicate the keys that are located in the front of the autosampler and more precisely keys 1 2 3 4 5 6 and 7 see figure 2 Directional keys The keys in front of the autosampler allow communication and control of the autosampler The autosampler display has two lines as shown in the following figure Screen The cursor is active in that line when it is blinking on the extreme right hand side To move from one line to another press to go down or to go back up These keys O also m
34. 5 2 1 HT300A User manual Pressing Y enters the following screen Pre inj Dw sec 4 Post inj Dw sec Screen 30 Field sets the standing time of the syringe needle inside the injector before making the injection Field sets the standing time of the syringe needle inside the injector after the injection this allows the temperature of the syringe to equilibrate with the temperature of the GC port Both field O and field may have a value between 0 and 99 seconds Use of internal standard If the method uses an Internal Standard technique the autosampler will display screens 26 and 27 rather than screen 25 Screen 26 is shown below Fill Spd pls lt IntStd Vol pl v Screen 26 Field O is the aspiration speed of the syringe in ul s The aspiration speed has to be between 200ms minimum time and 200s maximum time So the range of values that the field may have is function of the volume and it will be between a min speed and a max speed of Vol to aspire Vol to aspire Min Speed 3 Max Speed 200 s 0 25 To inject in the quickest time possible about 80ms set the speed to high Press to select field This is the quantity of internal standard indicated in ul to collect This value is variable from 0 to 500 ul Pressing 6 enters the following screen AirGap Mode AirGap pl v Screen 27 HT300A UK B doc 5 5 HT300A User manual Field selects the
35. Air Gap Mode see Glossary It may have the following values AirGap is exclusively interposed between the Internal Standard and the Sample There are two AirGaps the first one before the sample the second one after the sample Field selects the volume of the AirGap in ul It may have a value between 0 and 500 ul Press 2 and C to increase 2 or to decrease C this value in steps of 0 1 ul or in steps of 1ul for values higher than 10ul Press to go to screen 28 From this point on the displayed screens are the same as those where an Internal Standard is not used HT300A_UK_B doc 5 6 5 2 2 HT300A User manual Exit from method mode Exit with SAVING e Itis necessary to reach screen 30 press 0 or press EXIT Screen 30 will be displayed as below Press ENTER to save 4 Copy Method Das Screen 31 Field O will show the method in use or the method that has been modified Field shows the position to write the new method it can be written over the old file or press 2 or to save it under another number This feature allows to the user to cut and paste some parts of the method Pressing ENTER displays screen 20 e To exit press the key This will display the main menu page Run Time Menu gt EXIT without SAVI NG e Reach screen 31 pressing the keys or by pressing EXIT twice e Press key Then will display main menu page Run Time Menu HT300A_UK_B doc 5 7 5 3 5 4
36. DLE Screen 65 Rear Inj Alignment TURRET STO NEEDLE Screen 65A Rear Inj Alignment STO Y O DEPTH Screen 66 For the description of the screens 65 65A and 66 please refer to screens 63 63A and 64 respectively please note that the screens 65 65A and 66 define the set up of the second injector From screen 61 press EXIT to revert to screen 50 AN Warning Take care to ensure the following procedures are met In particular ensure that e The needle is aligned over the centre of the needle guide on the injector e The needle guide fits properly together the injector e The needle has to enters the injector without trouble and penetrates the diaphragm without an excessive bending If it does not check the injector septum type of syringe used and needle tip type e when the needle is in the port ensure that it has a little further room to go down about 1 2 millimetres In this position wait for about 20 seconds HT300A UK B doc 4 9 4 6 HT300A User manual before pressing to memorise the position The operation is correct if within this period of time screen 00 doesn t appear If screen 00 is displayed the system has found anomalies To use 00 please go to paragraph 4 3 Manual Operations Vial Syringe Set up The following screen will be displayed Vial Size m Vial Depth mm Screen 67 Field displays the capacity of the installed vial indicated in millilitres This can be
37. IONS Syringe Washing Position the cursor on the Syringe Wash line see screen 05 paragraph 6 1 Press 2 to enter the submenu Set up The following screen will appear WASH SET UP Sol vent O y Screen 07 Use 3 and to select the correct solvent to wash the vial This may be A B C or D corresponding to the two solvent bottles situated either side of the waste bottle see figure 4 Press to enter the following screen Volume pl Wash Repeat lt gt Screen 08 Field O corresponds to the quantity of solvent that has to be used It may have a value between 0 1 ul and nominal value of the syringe installed Press 3 and to increase 2 or to decrease this value in steps of 0 1 ul Field corresponds to the number of washes to make This may be between 1 and 15 Press and to increase 9 or to decrease this value Pressing at any moment will go back to the main menu Run Time Menu without saving the changes HT300A_UK_B doc 7 1 7 1 2 7 2 L 7 A af HT300A User manual Run Press START from screen 07 or 08 to start the syringe washing procedure During this procedure the display will be alternate between screens 07 and 08 The number of washes to be made will be displayed NOTE To stop the washing procedure in any moment press STOP As soon as the injection procedure is finished the unit will revert to screen 05 see paragra
38. ITO TC Errore Il segnalibro non definito Lack of waste bottle 00000000 0000000000000000 000000 ena 0ane Errore Il segnalibro non definito Vial with wrong dimensions Errore Il segnalibro non definito Autorun errors sseni sssssse Errore Il segnalibro non definito Lack of waste bottles see Errore Il segnalibro non definito Lack of solvent bottle Programming Errors Errore Il segnalibro non definito Errore Il segnalibro non definito Damages to syringe needle nne agen serene ne venenenenenene nononono nennen senen enenenenenenenenenenenenenenenenenene 9 7 MAINTENANCE Ordinary Maintenance eegener SEENEN Extraordinary Maintenance ennnnnese GLOSSARY nn CONSUMABLES en SYEINBE rien binaan inna Vial Solvent bottles waste Appendix C FLOW DIAGRAMS Set up mode ne Sequence modems Aka OS hun BD Ec S Runtime mode ms Appendix D SCREENS LIST Start Up estt rte hs Run Time Menu Syringe Washing Single Injection panan angen anan nangani sauyun aka pedale estos deest sen dasanan teu vereende seen eodd HT300A UK B doc i HT300A Usr manual 14 5 Automatic E 14 6 Priority Injection 14 7 Mog m 14 8 Method Miles 14 9 Sequence Mode 14 10 Error Messages sie Se SCREENS AND FIGURES INDEX dE ta
39. NDL TUR TRY TCH bsy AY AY AY O PLG NDL TUR TRY TCH AY bsy AY AY O PLG NDL TUR TRY TCH AY AY bsy AY O PLG NDL TUR TRY TCH AY AY AY bsy O Check the unit for obstacles and remove if necessary Use to select the part to move and and to move the part that is blocked For further details on manual movements please refer to paragraph 4 3 Manual Operations where you will find the description of screen 00 Damage to syringe needle Damaged syringe needles are normally symptoms of a problem relating to the type of vials syringe needle or type of injector septum used they may also be caused by the an incorrect syringe installation configuration It is recommended 1 Try to identify the cause of the damage and the position in which the damage happened Ensure the alignment on the sample vials and on the injector s see paragraphs 4 4 and 4 5 were made correctly To check HT300A_UK_B doc 9 7 HT300A User manual this examine the septa of the sample vials and injector are correctly perforated 2 Check that syringe was installed correct way as indicated in paragraph 4 7 and ensure that all instructions and notes were followed In particular check that the needle guide was correctly aligned with needle tip as indicated in following figure 3 Verify the specifications of the syringe with that supplied in Appendix B Consumables In particular check that the needl
40. RIPTION sense 2 1 Control panels t s ana ena naen Kana Na ada na aana ee Socio banda naga Connection panel Solvent and waste vials Autosampler base Vials loCatiOI eniro eate sadana ea nn nig gena anak anana bac Ee rE gagang na ine ae aee nen gini Syringe IN ee Moving Parts Control panel descriptiO scccssiacescsscsccssoveccsseoosaseooonsnavososeesoosseessessnsosesvenseensubesesosssnesbesssoesseasonsess 2 9 Typographic NOTES T C J H 2 9 Directional keys vens sess STOP START EXIT and ENTER Keys ssssensnnnnnnnnennennneense SET MET and SEQ keys e esssseoscscescocososcososcoeossseoecesoceecososcocoscoeososeoeessssecsososeocososcosossseossssesessose STATUS indicators INSTALLATION seen Unpacking and preliminary operations Autosampler assemDly sssseraversssnersndensendsedensserssnootensdoroserer ie een sona nen Pocket versi n sesscccedscccceccosusecesechudeesssssecsscuccondcsececeusecensuoastseucboueussassocsssesonseadsscsessasesosedsssucesscessadseusogs Standard version AO eR Checking and voltage modification Installation on the bracket Electrical Connections eerte Tray installation with the autosampler OFF Introduction Set up Mode Manual Operations Actual Parameters sise Visualisation of parameters for a
41. art the single injection procedure according to the set parameters During this procedure the display will cycle through screens 9 10 and 11 Press the STOP key to stop the injection at any time As soon as the injection procedure is finished the unit will revert to screen 05 see paragraph 6 1 Automatic Run Position the cursor on Automatic Run see screen 6 paragraph 6 1 and press 2 to enter the submenu Set up The following screen will appear First Step O lt gt HT300A_UK_B doc 7 3 HT300A User manual Last Step O B Screen 12 Press 2 and C to select the first step of the sequence to set up or modify a sequence please refer to paragraph 5 3 Field may have values between 1 and 16 To change field press This selects the last step of the sequence Field may have a value between 1 and 16 the Last Step must to be the same or more than the First Step NOTE It is only possible to select existing steps numbers corresponding to non existent steps will not be displayed Press to enter the following screen End seq Output amp PowerOn Restart Screen 13 Field manages the END Sequence output Press and to select either The sampler emits a signal from the GC and AUX YES connecters at the end of a sample sequence NO The sampler does not emit a signal from the GC and AUX connecters at the end of a sample sequence Field may assume the following val
42. as shown in figure 8A 2 With the same screwdriver extract the fuse compartment as shown in figure 8B 3 Replaces the two fuses with others with the right amperage see table below Kind EN 60127 115v 0 8AT 230v 0 5AT Table 1 Insert the fuse compartment with the correct voltage at the top 5 Close the compartment by pressing gently If the fuse compartment is correctly inserted the selected voltage can be read 3 3 2 Installation on the bracket HT300A UK B doc 3 4 HT300A User manual Warning Follow the instructions with the autosampler switched off Moving the unit with the autosampler switched ON can cause serious damage to the autosampler Please follow these instructions in the following order 1 Place the auto sampler on the bracket paying attention that the four holes identified as A in the figure 1A or 1B fit the bracket holes Fix the auto sampler on the bracket using the four screws and the washers supplied with the mounting kit The two taller screws have to be fixed on the front side Screws the autosampler on the bracket leaving the 4 screws loose so the position of the autosampler can be adjusted Open the Syringe location removing the safety screw with the supplied Allen key and open the protective lid see figure 7A e 7B Adjust the safety lock AN Warning If you don t adjust the safety lock the autosampler after the start up displays an error and stops see paragraph
43. at the needle guide moves freely To do this lower the lodging NDL slowly and carefully until 2 3 cm of the needle protrudes into the waste vial The needle should not bend If the needle guide doesn t move correctly and the needle bends then repeat the installation procedure paragraph 4 7 1 Press to exit screen 00 The will go to screen 69 Select Vial Syringe Set then Touch amp Plunger Zero Run Injection Setup see paragraph 4 5 Complete the procedure and exit from Set up mode From screen 50 press Close the Syringe Lodging lowering the lid and locking with the protective screw using the key supplied see figures 7A and 7B 4 7 4 Replacement of the syringe lodging To replace the syringe lodging The syringe lodging has to be empty Remove syringe block Remove the piston block Using the screwdriver supplied with the autosampler unscrew the central positioning screw of the syringe lodging Remove the syringe lodging Insert the new syringe lodging checking that the intermediate needle quide is in the correct position and is inside its guide Shut the central position screw of the syringe lodging HT300A_UK_B doc 4 15 Chapter 5 2 HT300A User manual 5 PROGRAMING 5 1 Method Mode To enter the method mode press the key on the front left side of the unit This mode displays creates or modifies a method Each method is a list of parameters that define each analytical parameter involve
44. cedure must be performed by trained personal only users are kindly requested to check if the autosampler has the proper configuration To do this it is necessary to check indications contained on the Product Description supplied with the autosampler HT300A_UK_B doc 2 4 HT300A User manual 2 1 5 Vials location Depending version see Chapter 1 par Versions the autosampler can mount different kind of trays fixed or rotating with different capacities 10 40 or 110 The trays are always located on the side of the autosampler Fixed trays Pocket version allows the use of 10 position fixed tray Figure 6A Pocket installed On the left hand side Figure 6B Pocket installed On the right hand side Each vial is identified by a number from 0 to 9 on the pocket Rotating trays Standard version allows the use of 110 location rotating tray Figure 6C HT300A_UK_B doc 2 5 HT300A User manual Vials on the 110 locations rotating trays are organised in 10 sectors see Figure 6D Vials on the 40 locations rotating trays are organised in 10 sectors see Figure 6E Figure 6E Each vial is identified as a number of two digits This number is structured as follow Number Number identifying the identifying the sector position The Sector number between 0 and 9 is written on the central part of tray The position number of the vial inside a sector is written close to vial This number
45. ception of the signal GC Ready At the beginning of the At the contemporaneous movement of the syringe reception of the signals GC plunger Ready and SYNC IN The second row press to access displays the operation mode of the autosampler Field which can be changed using gt and may have the following values HT300A_UK_B doc 6 2 6 2 HT300A User manual Front It injects only in first injector Rear It injects only in second injector It injects the same sample in both injectors S For use in Confirmation Mode see Glossary chapter 11 It injects different samples in both injectors To use in Highthroughput Mode see paragraph 8 1 Hithrou NOTE Field is modifiable only if the system is configured for two injectors If the system is configured for only one injector it will be show the default option Normal and it will not be possible to change it Press to display the following screen SYRI NGE WASH SINGLE INJECTION d Screen 05 Press it goes to the following screen SINGLE INJECTION AUTOMATI C RUN Screen 06 Position the cursor use 00 on one of the three options SYRINGE WASH SINGLE INJECTION AUTOMATIC RUN and press gt to enter the respective submenu see chapter 7 BASIC FUNCTIONS Tray installation with the autosampler ON If the tray is missing or not properly installed it will generate the following error message TRAY O Removed Waiting Tray
46. cessary to check or to change the injection parameters then press to go to the following screen PRIORITY INJECTION Method Number O Y Screen 15 Press and C to set the method number Then press down to set the other parameters Sample number O HT300A UK B doc 1 HT300A User manual Int Std Sol vent Y Screen 10 Pre Wash Solvent A Post Wash Solvent ar Screen 11 Please refer to the Single Injection of this chapter for definitions of these screens After setting the correct parameters go to point 2 Press EXIT at any moment to go back to screen 14 without executing the priority injection but saving the modifications made POINT 2 To activate a Priority Injection it is necessary to change field 5 of screen 14 Stp Met Smp Rpt Pri p 0 0 Screen 14 Field displays as default NO Change this to YES using 0 O to activate the priority injection Priority injections are automatically made once the current running injection has finished During the priority injection screens 15 10 and 11 will cycle as per the single injection Pressing at any time will stop the process and the autosampler will empty the syringe if full and return to its central position Once the priority injection has been made the autosampler will display screen 14 and start automatic injection procedure from the point of interruption HT300A_UK_B doc 7 8 Chapter HT300A User manual 8
47. creen 01 After 5 seconds the autosampler will move the tower into the stand by position and proceed to recognise the type of vial tray The unit will display TRAY Identifying PLEASE WAIT Screen 02 This operation may take a few minutes Press EXIT to stop the tray identification procedure and confirm the tray type has not changed from the tray typed previously used do not do this on the first run When the tray has been identified it will show the main menu see paragraph 6 1 For any problems related to tray installation or removal refer to paragraphs 6 2 and 6 3 3 4 Switch off Before switching off the autosampler ensure that e The unit is in the main menu Run Time Menu The autosampler is on stand by status STAND BY If the autosampler is working stop it before switching off If it is in a menu different from the Run Time Menu press EXIT and the relevant menu key to return to it before switching off the autosampler In an emergency the sampler can be switched off in any position working status hold and from any position main menu secondary menu HT300A_UK_B doc 3 8 Chapter 4 1 4 2 HT300A User manual 4 SET UP Introduction To align the autosampler on the GC and to set the different kind of accessories vial and syringes itis necessary to operate in the SET UP Mode ZA Warning Set up must be completed carefully by trained personnel to prevent damage to the syringe or to the autosampl
48. ctor 2 Front Inj Rear Inj For 2 inijectors 1 Injector press ENTER to 2 Front Inj Rear Inj store the position in Injector Alignment 2 memory TURRET STO DEPTH Injector Alignment 2 TURRET STO NEEDLE the correct depth then press A i ENTER to store the position Vial Syringe setup Vial Size v M E A v Vial Depth A Syringe Size v PLG NDL TUR TRY TCH Syringe install AV AV AV AV i Touch Zero Plunger AUTOMATIC START MANUAL 4 STO AUTOMATIC TESTS ZERO PLUNGER OVEN TOUCH VIAL 1 TOUCH Flow Diagram of the SETUP mode HT300A UK B doc 132 HT300A User manual 13 2 Sequence mode Select the sample list or a sample batch for automatic run Method Number 0 9 Select the sequence step to edit You can not add steps to the sequence with this command Set the method number that is required for this step First Sample Choose the first sample to be injected Last Sample Choose the last sample to be injected Int Std Solvent Set the internal standard or solvent to be injected into the sample vial if an internal standard technique is used Sample Repeat 0 9 ph Set the number of times each sample should be injected Pre wash solvent A B C D Select the solvent for the prewash if used Select the solvent for the postwash if used Post wash solvent A B C D e NXT Next add a new step to the end of the sequence regardless of which step
49. d in the injection The autosampler can store up to 10 methods each method has a number between 0 and 9 Methods can be stored by editing a generic configuration which are different from the one used during the normal run for instance with a different syringe volume However if the method is not compatible with the configuration stored such as trying to inject a volume larger than the stated syringe size the system will give an error To proceed it will be necessary to change the installed configuration e g to replace the syringe with another one with a greater volume or to change the method itself Creating a method Press the key to enter the method mode The key will light and the unit will display the following screen Method Number O Internal Std v Screen 20 The field identifies the method number and can be changed using 3 and C It must have a value between 0 and 9 Changing this field recalls the set up information previously stored in memory for this position The second line to get it press authorises the use of an Internal Standard use 2 and C to set between Yes No This can have the following values Internal Standard Techniques are authorised Internal Standard Techniques are not authorised HT300A UK B doc 5 1 HT300A User manual Press amp enters the following screen Pre Wash O A Volume pl v Screen 21 Field selects the number of prewashes This number can be chan
50. de You can use vials of different volumes and interchangeable Trays with different capacity trays can be fixed or rotating positioned on both right or left hand side A large number of different syringes from 1 to 500 microliters 75 ml for special versions The autosampler can work in many different modes wash with sample and or with solvent pumping to eliminate air bubbles aspiration and injection speed controlled use of Internal Standard The sampler can use four different solvents located in four vials for the syringe wash A large vial is dedicate for waste HT300A_UK_B doc 1 3 1 5 1 6 HT300A Usr manual The autosampler can be controlled using a PC connected using the RS 232 interface or using the control panel on the front The user interface is easy to use and has a step by step operation It s possible to store the most frequently used settings in order to quickly and simply configure the unit Versions L HT300A is actually available in 3 versions HT300A HT300A 10 Pocket HT 300A LV HT300A Standard Version It is the standard version for gas chromatography In this version the autosampler has a rotating tray with 110 vials capacity HT300A 10 Pocket Version The Pocket version is for gas chromatography The only difference from the previous system is that this one has a fixed tray with 10 vials capacity HT300A LV Large Volume Version The Large Volume Version is again for GC The di
51. disconnect the power cord and contact an HTA representative for a product evaluation Do not attempt to use the autosampler until it has been evaluated Electrical damage may have occurred if the autosampler shows visible signs of damage or has been transported under severe stress Damage can also result if the autosampler is stored for prolonged periods under unfavourable conditions e g subjected to heat water etc Always disconnect the power cord before attempting any type of maintenance Capacitors inside the autosampler may still be charged even if the autosampler is turned off The autosampler includes a number of integrated circuits These circuits may be damaged if exposed to excessive line voltage fluctuations and or power surges Never try to repair or replace any components of the autosampler that is not described in this manual without the assistance of an HTA representative Syringe installation and removal operations are excluded from the above limitation HT300A_UK_B doc 1 1 HT300A Usr manual 1 1 Manual Structure In order to use correctly this autosampler and to obtain the best results from it read carefully this manual before using the Autosampler The manual is organised as follow Chapter 1 Chapter 2 Chapter 3 Chapter 4 Chapter 5 Chapter 6 Chapter 7 Chapter 8 Chapter 9 Chapter 10 Appendix A Appendix B Appendix C Appendix D Introduction Warnings and general characteris
52. dry cloth Do NOT use solvents detergents or spray on electrical parts Extraordinary Maintenance For any other maintenance operation not indicated in this manual please contact your Supplier or Technical Assistance Centre HT300A_UK_B doc 10 1 y b T ee af Appendix HT300A User manual 11 GLOSSARY Air Gap Am air gap is a cushion used for Internal Standard Techniques It may be interposed between Internal Standard and the sample mode post or it may follow both Internal Standard or the sample mode double Dual injection modes Confirmation Mode High throughput Mode High throughput mode is a high productivity technique which injects different samples into two separate injection ports Confirmation Mode Technique injects the same sample into two injectors to confirm the analysis Internal Standard This is an analytical technique that injects sample and a reference standard at the same time The reference is drawn in first to the high part of the syringe with the sample in the lower part Injection sensor Injection sensor is also called the Touch sensor It s used by the autosampler to detect when the transport needle guide locator have touch a surface or obstacle HT300A_UK_B doc 11 1 E on Appendix 12 1 12 2 Syringe 12 HT300A User manual CONSUMABLES The following table indicates the dimensions of the syringe and the reference number of
53. e is the same as the preceding one Press to select the second line which is displays the identifying number of the method to use for the step Field modify with using 9 and C will have a value between 0 and 9 Pressing Y enters the following screen First Sample O Last Sample Y Screen 34 Field O to selects the first sample vial to run Field selects the last sample vial These fields will have the values 00 01 99 and than the value OP 1P 9P corresponding to the identifying letters of the selected vial see paragraph 2 1 5 NOTE The selected vial has to be availble on the installed vial tray for example selecting vial numbers above 0 on the pocket will produce an error Pressing Y enters the following screen Int Std Solvent Sample Repeat Screen 35 Field selects the vial or solvent bottle to use as the Internal Standard If the chosen method doesn t include the Internal Standard field is not enabled and it will display If a standard is selected then this field can have a value of 00 01 99 OP 1P 9P corresponding to the identifying letters of the vial to select or A B C D corresponding to the identifying letters of the solvent bottles see paragraph 2 1 3 and 2 1 5 Field Y selects the number of times that the same sample has to be analysed This quantity is modifiable and it may have a value between 1 and 9 Press S and to increase 2 or to decrease
54. e used has a tip suitable for the septum to pierce 4 Verify suitable septa were used 5 Check that there are no obstacles inside the vial s such as an insert HT300A_UK_B doc 9 8 Chapter HT300A User manual 10 MAINTENANCE 10 1 Ordinary Maintenance 10 2 The autosampler doesn t require periodical maintenance except for emptying of the waste vial and the cleaning of the tray and tower Cleaning the waste bottle Go to screen 3 Change the indication TURRET ON setting it to Tray to easily access the waste bottle For further information on this operation please refer to chapter 6 e To remove the waste bottle raise by hand and remove with care if caustic substances have been used wear appropriate hand protection Remove off the plastic lid e Empty then wash the glass container and its plastic lid e Remount the lid and replace onto its original seat the same septa can be reused or replaced Cleaning of vial tray e Remove the tray from its seat see paragraph 6 3 Removing a Tray e Clean it with care do not use an abrasive detergent solution e Dry with a dry cloth External Cleaning of cabinet e Switch OFF the autosampler e Remove the power cable from the rear e Pay particular attention to the display the command keys and to fragile parts For their cleaning use only a damp cloth e Clean the cabinet using a non abrasive detergent solution e Dry with a
55. een 53 it goes to screen 54 Needle in Speed O A Injector Depth mm Screen 54 Field O will shows the injection speed into the injector This speed is indicated with a value between 0 and 8 NE 8 52mms en 4 poo 32mms Table 2 Field displays the needle s descent depth into the injector in millimetres Pressing EXIT at any moment it will revert to screen 50 Visualisation of parameters for two injectors From screen 53 it goes to screen 55 Needle in Speed O A Front Inj Depth mm Y Screen 55 Field O displays the injection speed into the first injector This speed is indicated by a value between 0 and 8 Set Set Value Corresponding Speed Corresponding Speed 5 ee Ae ee ee HT300A_UK_B doc 4 4 4 5 HT300A User manual 6 128 mm s NE p 512 mm s IC l Table 2A Field displays the needle s descent depth into the first injector indicated in millimetres Pressing enters the following screen Needle in Speed Rear Inj Depth mm v Screen 56 In this screen there is information about the second injector all notes given for the preceding screen are valid also for this one Pressing the EXIT in any moment it will come back to screen 50 Injection Set up AN Warning It is necessary to install the syringe before proceeding with the operations described in this paragraph The following screen appears Injection Setup Injectors Numbers
56. er If some values in the fields are incomplete or incorrect the led will remain light even after you exit from the Set up menu Set up Mode The Set up menu it is only available from the screen 01 From 03 it is possible to go back by pressing the EXIT key From screen 01 press the key to enter the set up mode the key will light and the following screen will appear Manual Operations Actual Parameters d Injection Setup gt Vial Syringe Setup Screen 50 Note rows 3 and 4 can be displayed only after pressing the key Screen 50 represents the main menu of the Set up mode The 00 keys can be used to place the cursor on one of the four lines corresponding to the options HT300A_UK_B doc 4 1 4 3 HT300A User manual Manual Operation Actual Parameters Injection Setup Vial Syringe Setup These submenus are accessed using the 2 key Manual Operations allows manual movement of the autosampler motors see Manual Operation Selecting Actual Parameters displays the parameters currently stored for injectors vials and syringe see Actual Parameters Selecting Injection Setup allows the needle speed into the injector s to be set and the alignment on injector s see Injection Setup Choosing Vial Syringe Setup sets the parameters of vials and of syringe see Vial Syringe Setup To exit from Set up menu it is necessary e
57. fference with the standard version is that it has a 40 vial tray for large volume samples It has a bigger syringe with volume from 0 1 ml to 10 ml and for special applications up to 75 ml Technical Specifications HT300A technical specifications Dimensions Height 520 mm Depth 400 mm Width Pocket version 270 mm Width Standard version 420 mm WEIGHT Pocket Version 8 kg Standard Version 9 kg ELECTRICAL SPECI FI CATI ON Voltage 230 115 10 Vac Frequency 50 60 Hz Power 160 VA HT300A_UK_B doc 1 4 b pa INTERFACE SPECI FI CATI ON To GC ENVI RON CONDI TI ONS Temperature Humidity ANALI TI CAL PARAMETERS Pre amp Post Wash Volume Number of washes Wash mode Wash with sample Sample wash Bubble elimination Bubble elimination volume Sampling withdrawal Sample Volume Air Volume Aspirating speed Viscosity time Injection Injection speed Waiting time before and after injection Internal Std Techniques Int STD Volume Air gap Volume Mode SOFTWARE OPZI ONAL HT COMSOFT A HT300A UK B doc HT300A Usr manual Cable or dedicated interface 15 C 35 C 5 85 from O to 9 9 ul 0 1 ul stepwise from 10 to 500 ul 1 ul stepwise up to 15 each injection each sample or sample group up to 15 up to 15 strokes from O to 9 9 ul 0 1 ul stepwise from 10 to 500 ul 1 ul stepwise from O to 9 9 ul 0 1 ul stepwise from 10 to 500 ul 1 ul stepwise from O to 9 9 ul 0 1 ul stepwise from
58. ged and may have a value between 0 and 15 Press 2 and C to increase 2 or to decrease C this value in steps of 1 unit Press to select the second line to set up the quantity volume of solvent to use for each pre washing operation Field may have a value between 0 ul and 500 ul Press and to increase or to decrease C this value in steps of 0 1 ul or in steps of lul for values higher than 10ul NOTE If the set number of pre washes is 0 it will not be possible to change field O so it will have the default value Pressing Y enters the following screen Post Wash O gt Volume pl Screen 22 This screen is similar to screen 21 and refers to the number of post washes and the volume of solvent to use for each post washing operation Screen 21 instructions are valid also for screen 22 Press to set the Pre and Post Washing modes Pre Wash Mode O gt Post Wash Mode Screen 23 Field O can be changed using the keys 3 and C may have the following values Injec Pre washing is made for each new injection Pre washing is made for each new vial Sample Pre washing operation is not made during the repetitions of the same vial Pre washing is made for each new step of the sequence HT300A_UK_B doc 5 2 HT300A User manual Field can be changed using the keys 2 and C may have the same values of field D shown above If the set number of post washes is 0 it will not be poss
59. ible to change the pre n washing mode Field will have the default value Press to set the quantity of sample and air to aspire Sample Vol ul O Air Vol pl Screen 24 Field O defines the volume of sample to aspire by the syringe to make the analysis Field may have a value between 0 ul and 500 ul Press 2 and to increase 2 or to decrease C this value in steps of 0 1 ul or in steps of 1ul for values higher than 10ul Press to select the second line that sets the quantity volume of air to aspire after the sample Field may have a value between 0 ul and 500 ul Press S and C to increase or to decrease this value in steps of 0 1 ul or in steps of lul for values higher than 10ul If a method includes the use of Internal Standard pressing Y will not display screen 25 but screen 26 Please refer to sub paragraph 5 2 1 Use of internal standard If the method DOESNT include the use of Internal Standard pressing the autosampler will display screen 25 Fill Vol vd 4 Fill Spd uso v Screen 25 Field O defines the volume of sample to aspire by the syringe for washing with sample This field may have a value between 0 ul and 500 ul Press and C to increase 2 or to decrease this value in steps of 0 1 ul or in steps of lul for value higher than 10ul Press to select field to change using the keys and C the syringe aspiration speed indicated in
60. in an automatic injection procedure In order to use this Mode it is necessary to HT300A_UK_B doc 8 1 HT300A User manual 1 Set this mode in a sequence step To do this set screen 32 the Tray Configur value to one of the following 10H 40H or 110H NOTE When configuring a step it is necessary to define the first and the last vials to analyse see screen 34 In the High Throughput Mode the first and the last vials must be from the first half of the vial tray The autosampler will automatically select the vials to make injections from the second half of the tray 2 Authorise the use of High Throughput Mode It is necessary to set as Inj Mode the value Hithrou in screen 04 It is possible to use the High Throughput Mode only if both points 1 and 2 are followed If either of the conditions are not met the following events happen e No point is verified The autosampler will work normally according to the parameters set e Point 1 is NOT verified This generates an error message indicating the tray is incorrectly set up see screen E20 paragraph 9 7 1 e Point 2 is NOT verified If Inj Mode a value different from Hithrou the autosampler will work in the following way It injects ONLY into the first injector i ONLY the first half of samples It injects ONLY into the second injector Rear ONLY the first half of samples It injects in both the injectors the same sample It on injects the samples from
61. is not a fault The Error is generated if the autosampler was not able to follow the software instructions or to complete or make in the proper way a specific movement Finally the symbol under the word TCH indicates the touch sensor status this is a warning to the operator that the needle guide is touching a surface plane The needle guide reached the plane The needle guide is not touching the plane Pressing the EXIT key will return to screen 50 Actual Parameters In this mode is possible to control set up parameters as they were previously loaded by the operator The following screen will be shown Actual Parameters Syringe Size O ul v Screen 52 Field displays the current value in memory of the syringe size Pressing amp enters the following screen Vial Size ml A Vial Depth mm v Screen 53 Field displays the set dimension of sample vials in ml Field displays the needle draught depth inside the sample vial Pressing HT300A_UK_B doc 4 3 4 4 1 4 4 2 mert HT300A User manual If the autosampler is set to work with a single injector it will display screen 54 please consult the sub paragraph Visualisation of parameters for a single injector If the autosampler is set to work with two injectors it will display screen 55 please consult the sub paragraph Visualisation of parameters for two injectors n Visualisation of parameters for a single injector From scr
62. ischarge the syringe to waste if full and returns the turret to its initial position During this operation Abort Autorun will flash on the screen As soon as the autosampler is in its central position the display shows the following END SEQUENCE Total Injections Screen 19 HT300A_UK_B doc 7 6 7 4 as HT300A User manual Field O displays the number of injections parts of the cycle made This value does not included priority injections any injections made using both ports Confirmation or High throughput Mode are counted only once and not as tow separate injections Press EXIT to revert to the main menu see screen 5 6 paragraph 6 1 Priority Injection Priority Injection is used to make a single injection at the first available moment during the middle of a sequence of injections This injection can be any vial and use any injection method When an injection cycle is running the following screen is displayed Stp Met Smp Rpt Pri b OD 0 Screen 14 To make a priority injection 1 Set the parameters for the priority injection selecting the method the sample vial the solvent for Internal Standard if used the solvent for pre washing and post washing 2 Activate the priority injection procedure changing in YES field POINT 1 If the parameters for the priority injection are the same as for the sequence that is currently being made then go to point 2 If it is ne
63. lacing the syringe it is necessary to follow with care the following operations First installation l Besure that the autosampler is in standby status 2 Open the syringe location by removing the safety screw and lifting up the protective lid 3 Enter Set up mode refer to chapter 4 select the row Vial Syringe Setup see paragraph 4 6 Vial Syringe Set 4 f needed change the Syringe Size and Vial Depth depending on the syringe about to be install see paragraph 4 6 Use and 3 to enter the syringe installation mode Screen 00 please refer to paragraph 4 3 should be displayed Move plunger by using the command PLG to its lowest position Lower the Syringe location NDL about 50 mm from its highest point Loosen the Needle guide regulator block allowing it to slide freely EE HT300A_UK_B doc 4 12 HT300A User manual 10 Remove the syringe locker by using the screwdriver provided see figure 7C e 7D 11 Remove the piston locker pulling it out by hand or with tweezers 12 Lift the Syringe location close to its highest point 13 Check the Syringe location matches the syringe see appendix B Ensure the correct Syringe location is installed for that type of syringe see Appendix B The autosampler has two different syringe holders that are suitable for various syringe types as described in appendix B Check that the appropriate syringe holder is installed before mounting If the syri
64. ment TURRET STO NEEDLE Screen 59A Press ENTER to confirm or EXIT to exit from alignment mode without saving The following screen will appear Injectors Alignment STO amp mm DEPTH v Screen 60 Move the syringe holder using Y and in order to insert the needle into the injector at the desired depth Field displays the depth reached by the needle This value HT300A_UK_B doc 4 6 HT300A User manual which is variable from 0 to 99 max according to dimensional limits is negative as it is calculated with respect to the touch point Press ENTER to confirm or press EXIT to exit from alignment mode without saving The unit will revert to screen 50 AN Warning Take care to ensure the following procedures are met In particular ensure that e The needle is aligned over the centre of the needle guide on the injector e The needle guide fits properly together the injector The needle has to enters the injector without trouble and penetrates the diaphragm without an excessive bending If it does not check the injector septum type of syringe used and needle tip type when the needle is in the port ensure that it has a little further room to go down about 1 2 millimetres In this position wait for about 20 seconds before pressing to memorise the position The operation is correct if within this period of time screen 00 doesn t appear If screen 00 is displayed the system has found anomalies
65. n the wrong way with respect to your GC specification please contact your local supplier Without extracting the autosampler from the box but simply lifting up it you can access the two screws that hold the Tray bracket see paragraph 2 1 4 Autosampler base Figure 5A o 5B Remove the two screws that will fix the tray bracket The two screws are different if the tray bracket is configured on the right or in the left side please see Figure 5A for the right hand side 5B for the left hand side ju How to install the tray Bracket Take the tray bracket and install it on the autosampler base then insert the two screws previously removed and screw them firmly in place HT300A UK B doc 3 2 3 3 mert HT300A User manual Connect the flat cable from the bracket to the autosampler socket see Figure 5A o 5B Extract the autosampler from the box and place in a vertical position with the display facing forwards Now the autosampler is ready to be installed on the GC Take out the autosampler and its accessories from the box Installation Before using the autosampler please read carefully the contents of this chapter 1 8 9 10 11 Ensure the correct voltage is selected see paragraph Errore L origine riferimento non stata trovata Errore L origine riferimento non stata trovata Install the autosampler on the bracket see paragraph 3 3 2 nstallation on the bracket
66. nd reduce the set value of Sample Volume and or Air Volume e Press and replace the syringe with one having a higher capacity e Press and change the value set for the syringe in Set up if this is wrong with reference to nominal volume of the syringe SMP I S SLV vol max Met O Stp NXT Screen E26 Cause e The sum of the value set for Sample Volume volume of solvent used for the analysis plus the IntStd Vol Volume volume of Internal Standard is higher than nominal volume set for the syringe Solution e Press and reduce set value of Sample Volume and or set value of IntStd Vol Press and replace the syringe with one having a higher capacity e Press and change set volume for the syringe in Set up if this is wrong with reference to nominal syringe of the syringe Wrong IS SLV Range Met O Stp NXT Screen E27 HT300A UK B doc 9 6 9 8 9 9 HT300A User manual Causes e The vial chosen as Internal Standard is not valid E g Vial 99 has been chosen but the 40 position tray is installed Solution e Choose another vial or bottle as Internal Standard e Replace installed tray with the required one Mechanical Errors If the autosampler can not correctly complete a movement within 12 seconds of the command being made it will give an error message and re align the motors the display will revert to screen 00 The letters Bsy will appear under the part that is not moving correctly PLG
67. nge holder is wrong it will be impossible to insert the syringe or it will not sit properly in the unit To change the syringe holder refer to paragraph 4 7 4 14 Take the Syringe pointer supplied with the autosampler This tool has to be installed by leaning it on the waste bottle so that it fits with the waste In this way the Syringe positioning sensor will be interposed between the waste and the needle guide see Figure 7D 15 Insert the needle into the intermediate needle guide and then into the needle guide insert the syringe body into the holder and lastly position the plunger into its lodging 16 Replace the Syringe locker tightening the two screws carefully 17 Lower the syringe lodging NDL until it touches Syringe pointer As soon as it happens the needle starts to bend slightly 18 Shut the Needle guide regulator block previously loosened This operation assures that the needle point remains perfectly aligned with the needle guide and that the needle doesn t protrude out of needle guide This is necessary to prevent damage to the needle 19 Tighten the Safety lock For correct use this has to be lifted up about 10 mm higher than the needle guide regulator in order to work as a safety stopper 20 Check moving by hand that the Needle guide slides freely in the position described in Figure 8C 21 Raise the plunger PLG and insert the Piston locker ensuring that the appropriate face is used for the syringe type if in
68. nge this to YES using 00 to activate a priority injection with reference to the running injection cycle For further details on the set up of a priority injection please refer to the next chapter Press the key to display the type of synchronism and the mode in which the autosampler is working HT300A_UK_B doc 7 5 HT300A User manual INJ SYNC O INJ MODE Screen 04 After 3 seconds the sampler reverts to screen 14 Press the key to display the number of injections made by the autosampler in Autorun Mode priority injections are not counted Injections Carried out Screen 14A After 5 seconds the unit reverts to screen 14 When the injection cycle is finished the sampler displays the following screen END SEQUENCE Total Injections Screen 19 Field displays the number of injections performed This value does not included the injection s made in priority mode injections requiring using both injectors Confirmation or High throughput Mode are counted only once and not as double Press to go to the Main Menu see screen 5 paragraph 6 1 I nterrupting an injection To interrupt the automatic injection sequence at any moment press the STOP key The following screen will appear EXIT gt ABORT AUTORUN START gt CONTINUE Screen 18 To re start the injection cycle from the interruption point press START or to confirm that the sequence should be stopped press EXIT The sampler will d
69. nt emits sample instant signal or continue Flow Diagram of the RUNTIME mode HT300A_UK_B doc 13 5 Appendix HT300A User manual 14 Appendix D SCREENS LIST 14 1 Start Up HT300A Series Software rel O Screen 01 TRAY DENTI FYNG PLEASE WAIT Screen 02 14 2 Run Time Menu TRAY MODEL O TURRET ON Y Screen 03 IN SYNC INJ MODE v Screen 04 SYRI NGE WASH SINGLE INJECTION d Screen 05 SINGLE INJECTION gt AUTOMATIC RUN E Screen 06 14 3 Syringe Washing WASH SET UP Solvent O y Screen 07 Volume pl O Wash Repeat lt gt Screen 08 HT300A_UK_B doc 14 1 14 4 Single Injection 14 5 Automatic Run HT300A User manual SINGLE INJECTION Method Number O v Screen 09 Sample number Imt Std Sol Vent se Screen 10 Pre Wash Solvent O A Post Wash Sol vent ar Screen 11 First Step O e Last Step Ww Screen 12 End seg Output lt PowerOn Restart Screen 13 PowerOn Restart lt Empty pos Sync ar Screen 13A Stp Met Smp Rpt Pri pe o0 0 GO Screen 14 Injections Carried out Screen 14A EXIT gt ABORT AUTORUN START gt CONTI NUE HT300A_UK_B doc Screen 18 END SEQUENCE Total Injections Screen 19 14 2 HT300A User manual 14 6 Priority Injection PRIORITY INJECTION Method Number v Screen 15 Sample number Int Std Solvent Y Screen 10 Pre Wash Solvent O amp Post Wash Sol
70. ny user intervention ZERO PLUNGER WAIT RUNNING Screen 72 The units then sets the plunger ZERO point WASTE TOUCH WAIT RUNNING Screen 73 and the TOUCH POINT on the waste bottle VIAL 1 TOUCH WAIT RUNNING Screen 74 And finally the TOUCH POINT on vial 1 of the rotating tray HT300A_UK_B doc 4 11 4 7 4 7 1 ES HT300A User manual At the end of this procedure the unit lifts the plunger PLG to the nominal syringe volume Check that the bottom of the plunger is level with the top of the syringe scale If it is not ensure the syringe volume set in memory matches the syringe used If the error persists please contact your local supplier At the end of the procedure the autosampler will go automatically back to screen 50 To manually set the TOUCH POINT for the plunger press and keys to move the plunger toward the ZERO PLUNGER position When it has reached the position wait at least 20 seconds then press the ENTER key If the ZERO PLUNGER position set up is defined correctly screen 73 will be shown if not an acoustic signal will be emitted and screen 71 remains displayed From screen 73 the manual and automatic procedures are the same Note If the autosampler doesn t locate the waste vial or the vial in tray position 1 it will generate an error message Syringe Installation In order to avoid damaging the syringe the autosampler and or to the user when installing removing or rep
71. ove from one screen to another from a menu to another Keys 2C change the values on a specific lines They also access secondary menus Directional keys 0 O 2 C are not active in all screens The active keys are indicated by the icon on the extreme right hand side of the screen Extreme right Extreme right H hand side Active hand side Active 0 2 c 0 The combination of more than one icon indicate that more than one directional key is active If there is no icon then the directional keys are not active STOP START EXIT and ENTER keys EXIT allows movement inside menus EXIT is used to go back to a previous screen from the present one To exit from a secondary menu and go back to the main menu it is necessary to use EXIT STOP is used to stop the autosampler when it is moving When this key is used the autosampler goes from the active status RUN to hold STAND BY HT300A_UK_B doc 2 10 HT300A User manual START activates the autosampler ENTER confirms an instruction to the autosampler 2 3 4 SET MET and SEO keys accesses the configuration set up menu of the Autosampler goes to the menu that sets the injection parameters Finally enters the mode for defining a sequence of injections automatic run When the unit is in a sub menu the respective key will be lit To exit the menu it is necessary to press the same key that was used to access to the menu Exit is only possible from the first screen of
72. ph 6 1 Single Injection 7 2 1 Position the cursor on the Single Injection line screens 05 06 paragraph 6 1 and press to enter the submenu relative to single injection Set up The following screen will appear SINGLE INJECTION Method Number O y Screen 09 Use and to select the method This can be between 0 and 9 Press to show the following screen Sample number Int Std Solvent Screen 10 Field O is the sample vial from which to make the injection This field may have the values 00 01 99 OP 1P 9P corresponding to the identification of the vial see figures 6A 6B 6C 6D Field Y is enabled only if the method see screen 7 uses an Internal Standard otherwise it will display as default HT300A_UK_B doc 7 2 7 2 2 7 3 7 3 1 HT300A User manual When enabled it may be 00 01 99 OP 1P 9P or A B C or D if one of the solvent vials is needed Press to the following screen Pre Wash Solvent O A Post Wash Solvent ar Screen 11 Select using 2 and C the pre and post wash solvent field These may have values A B C or D corresponding to the two bottles either side of the waste vial it is possible to use the same solvent for each wash Pressing EXIT will revert to the Main Menu Run Time Menu without making the injection but with saving the modifications Run Press START from screen 9 10 or 11 to will st
73. r C A I NJ status indicator D B INJ status indicator N B For further information on the key board please refer to Paragraph 2 1 2 Connection panel HT300A_UK_B doc 22 HT300A User manual 2 1 2 Connection panel On the rear side of the autosampler there is the connection panel as described in the following figure 1 2 Figure 3 REAR PANEL Power setting 115 230 Vac I O Power switch Electrical Connectors 3 4 5 4 Power cable plug RS232C serial port GC Other devices connection GC elemental analyser Ground 2 1 3 Solvent and waste vials Over the Keyboard there are the solvent and waste vials U BWN Mm Figure 4 SOLVENT AND WASTE VIALS solvent A location solvent B location solvent C location solvent D location waste location sooo HT300A UK B doc 2 3 2 1 4 HT300A User manual Autosampler base je e je e e e e m Ae ei e e Sa El 18 A 6 ve KR OA amp de e Figure 5A Figure 5B UNDERSIDE THE AUTOSAMPLER UNDERSIDE THE AUTOSAMPLER with left hand tray with right hand tray Screws A The two screws used to fix tray bracket Connectors 1 Connector for the tray support Not used in the Pocket version 2 Aux Connector to connect an integrator As already stated the autosampler can be configured with the tray on the right hand side or on the left hand side This pro
74. te anssen REA UE ERIS dE SEENEN dE I HT300A_UK_B doc iv HT300A Usr manual 1 INTRODUCTION Read the following warnings before unpacking or using the unit Warning See Declaration of CE Compliance enclosed with the autosampler Before removing the autosampler from the box please read carefully paragraphs 3 1 un Unpacking and preliminary operations and 3 2 Autosampler assembly Before switching ON the autosampler check the correct voltage Electrical Hazards Every analytical instrument has specific hazards so ensure to read and comply with the following precautions They will help ensure the safe long term use of your autosampler Only use fuses of the type and current rating specified Do not use repaired fuses and do not short circuit the fuse holder The supplied power cord must be inserted into a power outlet with a protective earth contact ground When using an extension cord make sure that the cord also has an earth contact Do not change the external or internal grounding connections Tampering with or disconnecting these connections could endanger you and or damage the autosampler The autosampler is properly grounded in accordance with these regulations when shipped You do not need to make any changes to the electrical connections or to the chassis of the autosampler to ensure safe operation Do not turn the autosampler on if you suspect that it has incurred any kind of electrical damage Instead
75. tics Equipment description Parts names notes Control panel description Installation Preliminary operations Assembly Installation Sampler switch off Set up Configuration and set up of the autosampler Programming Method mode Sequence mode Run Time Menu Main menu guide Basic functions Single injection Automatic sequence of injections Advanced functions High Throughput Mode Management of errors Cause e solutions Maintenance Ordinary and extraordinary Glossary Consumables Flow diagrams Screen list HT300A_UK_B doc 1 2 1 2 1 3 1 4 3 Li HT300A Usr manual Applicable rules 73 23 CEE Low Voltage Rule 89 336 CEE Electromagnetic Compatibility Rule 93 68 Rules modification 73 23 e 89 336 CEE Symbols table Please read carefully this table before using your autosampler These symbols are used in this manual AN do not touch action forbidden warning check the manual AN High Voltage General specifications This isan autosampler for gas chromatography The HT300A autosampler is compatible with the majority of gas chromatographs It allows sample introduction in both packed and capillary columns The unit can be installed on GC s with one or two injectors Fir Gas Chromatographs with two injectors your autosampler can be configured to inject automatically in one or the other injector or in both of them in confirmation mode or in high throughput mo
76. uctions performing every connection A A Warning Check that the autosampler is switched OFF 1 Connect the analyser with the 9 pin port of the autosampler using one of the cables provided with the autosampler see figure 3 2 Plug the power cable in the autosampler and then insert it in the mains power 3 If it is necessary to work in remote mode plug the second cable provided with the autosampler in the port identified as RS232C see figure 3 to the serial port of the PC 4 If it s needed to use an integrator or it s necessary to use autosamplers in twin mode refer to the Aux connector under the autosampler base see paragraph 2 1 4 Autosampler base 3 3 4 Tray installation with the autosampler switched OFF 1 Hold firmly with one hand the central part of the tray 2 Place the tray on its bracket as shown in the following figure Le Figure 9A Figure 9B BEFORE INSTALLATION AFTER INSTALLATION 3 Gently rotate the tray clockwise until it reaches the home position If this operation was done correctly there will be no error messages switching on the autosampler A HT300A_UK_B doc 3 7 ES 3 3 5 Start Up HT300A User manual The main switch of the autosampler is on the back of the autosampler see figure 3 switch 2 switch it on I position As soon as the autosampler is switched on the following screen will appear where O is the software version HT300A Series Software rel S
77. ues If the power is lost during a run the sampler restarts YES PARA the injection cycle from the point of interruption If the power is lost during a run the sampler does not re start the injection cycle from the point of interruption Press EXIT at any time to revert to the main menu Run Time Menu annulling the operations made HT300A_UK_B doc 7 4 HT300A User manual Press to the following screen PowerOn Restart D A Empty pos Sync ar Screen 13A Field O is explained above Field handles a missing vial in a sequence It may have the following values If a vial is missing the sampler emits the sample instant signal to the analyser as if the vial was present If a vial is missing the sampler doesn t emit a sample instant signal to the analyser and skips directly on to the next vial 7 3 2 Run Press START from screen 12 13 or 13A to start the automatic injection cycle During this procedure the following screen will be displayed Stp Met Smp Rpt Pri p D Dd 9 3 Screen 14 Fields and o are automatically updated by the autosampler Field is the priority injection mode Fields and provide information on The running step of the sequence 0 The method in use for the current sample 9 The vial position currently running 9 The residual number of analysis repetitions of the same vial 0 Field displays NO as default Cha
78. ul s The aspiration speed has to be between 200ms minimum of checked time and 200s max time So the range of values that field will have is function of the set volumes and it will be between a min speed and a max speed HT300A_UK_B doc 5 3 HT300A User manual Vol to aspire Vol to aspire Min Speed 3A Max Speed 200 s 0 25 To inject in the quickest time possible about 80ms set the speed to high Pressing Y enters the following screen Sample Washes Pullup Strokes Y Screen 28 Field defines the number of washes to make with the sample It may have a value between 0 and 15 Press Y to select field This is the rapid up and down movements for the plunger to eliminate air bubbles from the syringe Field may have a value from 0 to 15 Pressing Y enters the following screen Visc Dly sec Inj Spd pls O Screen 29 Field O sets the waiting time at the end of the aspiration procedure It can be set from 0 to 15 sec Field indicates the speed in ul s which the syringe injects This must be between 200ms min checked time and 200s max time The values that field has is a function of the set volumes and it will be between a minimum speed and a maximum speed Vol to inject Vol to inject Min Speed SC S S Max Speed 200 s 0 25 To inject in the quickest time possible about 80ms set the speed to high HT300A UK B doc 5 4
79. utorun errors 9 6 1 Lack of waste bottle WARNI NG Waste Fail START gt Continue EXIT abort Screen E10 Causes e The waste bottle is missing Solutions e Insert a waste bottle and press START e Press EXIT to exit and to interrupt the Autorun procedure 9 6 2 Lack of solvent bottle WARNI NG Vial absence press EXIT then fixup Screen E11 Causes e The solvent vial selected for pre washing or post washing is missing see figure 4 Solution e Press EXIT The autosampler will enter screen 00 so the tower can be moved manually Insert the missing solvent vial s Press EXIT The sampler will re start the Autorun procedure from the point of interruption HT300A_UK_B doc 9 3 9 7 HT300A User manual Programming Errors The errors listed in this paragraph may happen during either single injection or 9 7 1 Automatic Run The display differs slightly in the top line but the lower line is the same Met O Stp NXT Next to the word Met field is the identifying number of the method in which the error occurred Next the word Stp field is the number of the sequence step in which the error occurred Pressing will display other errors if present Press EXIT to return to screen 5 From here correct the parameter s causing error message SURVEY Tray range mismatch Met O Stp NXT Screen E20 Causes e The type of tray in the method doesn t correspond to installed tray
80. vent ar Screen 11 14 7 Set up Manual Operations Actual Parameters Injection Setup Vial Syringe Setup Screen 50 PLG NDL TUR TRY TCH AV AV AV AV D Screen 00 Actual Parameters Syringe Size O ul v Screen 52 Vial Size O ml A Vial Depth mm v Screen 53 Needle in Speed D A Injector Depth mm Screen 54 Needle in Speed O 4 Front Inj Depth mmv Screen 55 Needle in Speed D 4 Rear Inj Depth mmv Screen 56 HT300A_UK_B doc 14 3 HT300A User manual Injection Setup Injectors Numbers O Screen 57 Needle in SPEED O lt Injector Align Screen 58 Injectors Alignment TURRET lt gt gt NEEDLE Screen 59 Injectors Alignment TURRET STO Ai NEEDLE Screen 59A Injectors Alignment STO amp mm DEPTH yv Screen 60 Needle in SPEED D lt Front Inj Align d Screen 61 Front Inj Alignment TURRET lt gt NEEDLE Screen 63 Front Inj Alignment TURRET STO Ai NEEDLE Screen 63A Front Inj Alignment STO mm DEPTH v Screen 64 Needle in SPEED D lt Rear Inj Align 4 Screen 62 Rear Inj Alignment TURRET lt gt NEEDLE Screen 65 HT300A_UK_B doc 144 HT300A User manual Rear Inj Alignment TURRET STO NEEDLE Screen 65A Rear Inj Alignment STO DEPTH Screen 66 Vial Size m O lt gt Vial Depth mm Y Screen 67 Syringe Size Du A Syringe Instal d Screen 68 Syringe Install gt Touch amp Plunger Zero Screen 69 ZERO
81. y accessory provided e Check the product sheet to verify if the configuration is consistent with your GC as the Tray can be installed on the right or on the left of the autosampler with reference to the front view of the autosampler see Figure 1 e f the Tray is configured in the wrong way with respect to your GC specification please contact your local supplier e Without extracting the autosampler from the box but simply lifting up it you can access to the two screws that holds the Tray bracket Gee paragraph 2 1 4 Autosampler base Figure 5A o 5B e Remove the two screws that will fix the tray bracket The two screws are different if the tray bracket is configured on the right or in the left side please see the right Figure 5A for the right hand side 5B for the left hand side HT300A UK B doc 3 1 3 2 2 HT300A User manual 1 How to install the tray Bracket Take the tray bracket and install it on the autosampler base then insert the two screws previously removed and screw them firmly in place Extract the autosampler form the box and place it in a vertical position with the display facing forwards Standard version Place the box on a table Open the box then remove the upper foam packing Check the product sheet to verify if the configuration is consistent with your GC this because the Tray can be installed on the right or on the left of the autosampler see Figure 1 If the Tray is configured i

Liquid GC autosampler

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