BioDent User`s Guide
Contents
1. nt Indenter BioDent h i user s guide v 3 0 Active Life Scientific Inc Translational Research Tools for the Life Sciences BioDent is a trademark of Active Life Scientific Inc Active Life Scientific Inc 888 906 7770 Santa Barbara California www activelifescientific com table of contents 1 INTPFOCGUCTION cccecceeeeeeeeeeeeeneeneeneeneeeeeeeseees 5 1 1 Intended USE cceccecceeeeeeeeeeeeeees 5 1 2 BioDent Hfc System Technical Specifications cece ceceeeeeeeceeeeeeeeeeees 5 1 3 Safety Warnings and Precautions 5 1 4 Confidential Information and Computer Network Safety 7 1 5 Symbols and Abbreviations 8 2 getting Started ccccceeeeeeeeeeeeeeeseeeeeeeeeees 9 ZA UDP e 9 2 1 1 REQUIPE cccccccceceeeeseeceeeeeeeeeeteeeeaes 9 2 1 2 RECOMMENEGA ccceeeceeceeeeeeeeees 9 2 2 Setting It Up cece eeeeeeeeee ees 10 3 Operating INSTrUCTIONS cccceeeeeeeeeeeees 14 3 1 BioDent Hfc Control Software 14 3 1 1 Study Information ccceeeeeeeees 14 3 1 2 Measurement Protocol Setup 15 3 1 3 Sample Planning cccceeeeeeeeeees 16 3 1 4 Quick Report Setup eeeee 17 3 1 5 Measurement Console c008 17 3 1 6 Advanced Repott cccccceeeeeeeeees 21 3 2 Hardware ccceccecceeceeceeeeeeeeeeeeeeeeess 22 3 22. Professor Paul Hansma at the University of California Santa Barbara BioDent Hfc extends beyond the capabilities of conventional methods and provides a fine tuned solution specifically for the Life Sciences 29 theory of operation 6 5 what is BioDent Hfc measuring In review BioDent Hfc drives a Test Probe a microscopic distance into a material The forces achieved by the Test Probe while in contact with the material and the distance the Test Probe travels into the material relative to the Reference Point are time paired and recorded These Raw Data points are converted to newtons force and micrometers distance known as Converted Data points and plotted in real time on the F D Graph of the BioDent Hfc Control Software BioDent Hfc displays a suite of calculated parameters displayed as Calculated Data to inform the user of the contribution of the material s properties to resistance to indentation Measurement observations conclude that repetitive cyclical indentation testing and or high force high frequency indentation testing in the same location can provide valuable information about the contribution of material properties to an object s overall strength 6 6 indentation theory for BioDent Hfc BioDent Hfc uses a three phase indentation cycle of loading holding and unloading each phase comprising approximately a third of each cycle In the first third of the cycle BioDent Hfc attempts to reac
3. Reports and to create and save new protocols Remember you can neither save data nor access that study later in a Quick Study operating instructions Q note Unless you choose Temporary clicking will save your new or modified protocol to the Protocol Library and add it to the Select from Protocol Library drop down menu for later access If you click and are told Protocol Name Already Exists the protocol may already be saved to the Library If this happens click Use Existing select the protocol from the Select from Protocol Library drop down menu and click Q note Select Enable Pre Conditioning and define your pre conditioning protocol if the surface of your test material is either not easily identifiable or extremely rough e g material covered by other material such as bone covered by soft tissue There are three inputs to set Pre Conditioning Indentation Force N Pre Conditioning Indentations per Second Hz and Pre Conditioning Indentations per Measurement Cycles Find range and increment values for each in the Technical Specifications table 15 3 1 2 Measurement Protocol Setup This section describes how to access existing protocols from the Protocol Library create a new protocol or use a temporary protocol To use an existing protocol l Click Use Existing 2 Select the existing protocol from the Select from Protocol Library drop down menu BioDdent Measurem
4. activelifescientific com Lab Supplies Commonly used supplies may include protective gear gloves face mask etc surface coverings and sample handling apparatus tweezers tray getting started 2 2 setting it up Follow these simple steps to begin taking measurements Step 1 Turn on BioDent Hfc s Turn on BioDent Ensure there is nothing Turn on BioDent Hfc computer Hfc s monitor on Sample Plate Step 2 If applicable attach your Active Life approved application stage according to the instructions provided with the stage Don t attach the Probe Assembly or samples yet There are a few more steps Step 3 Open BioDent Hfc s Control Software by double clicking the desktop icon a BioDent Hfc 10 getting started Step 4 Do not force the MHU Ensure the button is fully pressed Pushing too hard may cause it to jam Contact Customer Care if assistance is needed customer care activelifescientific com Firmly hold MHU with Press button with your right While pressing button either your left hand thumb to release the MHU raise or lower MHU into positioning lock position 1 for larger taller samples or position 2 for smaller shorter samples Release button to lock position Step 5 Navigate through the BioDent Hfc Control Software see Chapter 3 until you reach the Measurement Console screen section 3 1 5 Then complete steps 6 and 7 Measurement Console St
5. be done correctly 2 Click O Measure You should now see Force Distance F D measurement data graphed in real time in the Measurement Console If you don t see an F D graph refer to the Troubleshooting chapter of this User s Guide see Chapter 4 BioDdent h Measurement Console 3 2 4 Continue Measuring the Same Sample in Different Locations Once the current measurement is complete indicated by a tone and the addition of a new row of Calculated Data in the Quick Report raise the MHU and reposition your sample to the next desired location Repeat Steps 1 and 2 until you finish taking measurements on the current sample 3 2 5 Measure Additional Samples Repeat sections 3 2 3 and 3 2 4 for remaining samples 24 operating instructions Q note See the Active Life Guidance Document titled Sample Positioning Stability and Probe Sample Guidance Document available at www activelifescientific com Always remove the Probe Assembly from the MHU and store in its original container before placing your hands in or near its path and whenever system is not in use chapter 4 troubleshooting 4 1 general considerations If your BioDent Hfc does not take measurements when you click O Measure check for the following common issues Power Is everything turned on Measurement Stand Reference Force Scale LCD Display Computer Monitor Mouse Check to see if there is an on off switch on
6. if this warning is received it is highly recommended to clean your probe prior to continuing the study troubleshooting 26 4 3 MHU 4 3 1 Unusual F D Graph curves Unexpected and unusual F D Graph curves may be caused by external influences including a damaged or dirty Probe Assembly improperly positioned sample and jarring of the instrument during a measurement For more information see the Understanding the F D Curves Guidance Document found at www activelifescientific com or contact customer care activelifescientific com 4 4 sample preparation 4 4 1 Accessibility of measurement area Some features on the sample may be inaccessible to the Probe Assembly For example when measuring through soft tissue the thickness of the soft tissue must be less than the length of the needle cannula on the Test Probe and or Reference Probe 4 4 2 Sample hydration To obtain accurate data from hydrated samples samples may need to be rehydrated throughout the study Especially for biological samples material properties can be heavily dependent on the hydration of the sample The sample can even be fully submerged in hydrating solutions during measurements while using BioDent Hfc and certain Probe Assemblies 4 4 3 Direction and orientation During measurement it s easy to lose track of where measurements have already been made Some approved Active Life Application Packages and Stages come with XY translation knobs that can help re
7. sample 2 Add Sample Location s Click Add to add the sample name location to the list Repeat for all samples in the study 3 Current Sample Location This shows the Samples Locations that have been added to your list a Toremove a Sample Location highlight it and click Remove 5 E P s Sample Planning 16 operating instructions Q note If you are unsure about the optimal settings for your protocol consult the Protocol Creation Guidance Document available at www activelifescientific com operating instructions Q note If you are currently following the Setting it Up instructions click here to return for the next step 17 3 1 4 Quick Report Setup Select up to five Calculated Parameters you d like to monitor throughout your study Click to proceed to the Measurement Console or Back to return to Sample Planning If you change your mind about the parameters you would like to monitor they can be changed during your study by clicking the Quick Report drop down menus on the Measurement Console screen Quick Report Setup 3 1 5 Measurement Console The Measurement Console is where you control BioDent Hfc when measuring samples and where you make the initial review of your data Indicators l Study Name Displays the name of the study that was entered or selected on the Study Information page If you selected J Quick Start no data is saved and this field is assign
8. the bottom of the mouse provided with your BioDent Hfc Keyboard Check to see 1f there is an on off switch on the bottom of the keyboard provided with your BioDent Hfc Cable connections Is everything plugged in Measurement Stand Power cable USB cables connecting to the computer and Measurement Stand MHU cable connecting to the MHU and Measurement Stand Computer Wireless or USB Keyboard Mouse 4 2 probe assembly considerations 4 2 1 Probe friction damage and corrosion Excessive Probe Assembly friction can cause inaccurate measurements and unreliable data Expect friction to gradually increase over time especially during long studies on hydrated samples and samples covered with hydrated materials However if Probe Assemblies are not properly cared for irreversible damage could be caused to your Probe Assembly before the end of its lifetime For more information see the Probe Assembly Care instructions at www activelifescientific com A bent or damaged Probe Assembly often caused by accidental mishandling or misuse will also cause friction Probe Assembly friction may be checked to ensure proper operation by following the Verifying Acceptable Probe Assembly Friction instructions at www activelifescientific com 25 Q note The BioDent Hfc Control Software will issue a pop up warning after a measurement is completed if Probe Assembly friction is too high Measurements will not be canceled but
9. 1 Zero the Reference Force Display 22 3 2 2 Prepare BioDent Hfc for Measurements cccccceceseeceeeeeeees 23 3 2 3 Prepare the Measurement Head Unit MHU for your First Measurement 24 3 2 4 Continue Measuring the Same Sample in Different Locations 24 3 2 5 Measure Additional Samples 24 4 troubleshooting ccseceeeeseeeeneeeeeeeeeees 25 4 1 General Considerations 05 25 4 2 Probe Assembly Considerations 25 4 2 1 Probe Friction Damage and COMPOS IO Mics ined erea EEn ree 25 4 3 Measurement Head Unit MHU 26 4 3 1 Unusual F D Graph Curves 26 4 4 Sample Preparation 0ccce0ee 26 4 4 1 Accessibility of Measurement Area 26 4 4 2 Sample Hydration cccccceeeeeeees 26 4 4 3 Direction and Orientation 26 4 5 Control Software cccccecceeeeeeeeees 26 4 5 1 USB Connection not Recognized 26 4 6 Measurement Stand 26 5 Cleaning care and maintenance 27 5 1 Life Expectancy cccccececseceeeeeeees 27 5 2 Periodic Maintenance 0068 27 5 3 Cleaning Instructions Precautions 27 5 4 Fuse Replacement 21 6 theory Of OperatiOn cccseceeeeseeeeeeeeeees 28 6 1 Introduction cece cece cece eeeeeeeeeeeaees 28 6 1 1 Measurement Head Unit MHU 28 6 1 2 Measurement Stand
10. at minimum before and after each study Follow instructions for a Performance Check at www activelifescientific com 5 4 fuse replacement To replace the instrument s fuse 1 Zi 3 27 5 3 cleaning instructions precautions aa Shut down the instrument and disconnect the power cord 1 Do not spray liquids directly onto the instrument Do not immerse Wipe instrument keyboard and mouse with a clean soft the instrument in liquid cloth dampened with a mild pH balanced detergent from the electrical power source Wipe again with de ionized water Wipe dry with a clean soft cloth Unplug the power cord from the power source Remove the power cord from the instrument Locate the fuse drawer at the power entry module where the power cord plug is connected to the instrument Remove the fuse drawer by pressing the fuse drawer latch upward and pulling the drawer out of the housing Remove both fuses from fuse drawer Replace both fuses with new fuses Use only designated fuses as indicated on the rear panel Replacement of fuses with other than the indicated fuses may cause a hazard and will void the warranty Ensure the fuse is properly seated in the holder Close the drawer and press it to lock into place 28 chapter 6 theory of operation Reference Point Indentation is unique This section describes the theory of Reference Point Indentation and the world s first commercial embodiment of the techn
11. c cccceeee 28 6 1 3 Probe Assemblies cccceceeeeee ees 29 6 1 4 Workstation cccccccececeeeeeeeeeeeeaees 29 6 2 Why Care about Material Properes ccsvisancatuwnumeconnarcevercoanenuwares 29 6 3 Conventional Methods 006 29 6 4 Reference Point Indentation Methods BioDent Hic 006 29 6 5 What Is BioDent Hfc Measuring 30 6 6 Indentation Theory for BIO DOWIE FO eee 30 6 7 Calculated Data cece ee eceeeee 30 chapter 1 introduction 1 1 intended use BioDent Hfc BioDent is designed for specific tasks making indentation measurements on calcified and hard materials ex vivo and in vivo BioDent Hfc is For Research Use Only Not for use in Diagnostic Procedures BioDent Hfc has not been cleared or approved for diagnostic procedures by the U S Food and Drug Administration FDA or any foreign equivalent 1 2 BioDent Hfc system technical specifications Measurement force range accuracy 0000000000 2 0N 10 0N 40 1N Measurement indentation frequency 00c00 2Hz Indentations per measurement cycles 06 1 20 cycles Pre conditioning indentation fOrce c cesses 0 5N 10N Pre conditioning indentations per second 0 1Hz 5Hz Pre conditioning indentations per measurement OY E ae S 1 20 cycles Resolution PON e seasonnipensahanqnteneseianeene 0 03N Do Cea E A lum Measurem
12. discovered 11 For the protection of service and shipping personnel instruments and S note accessories being returned for repair must be prepared in accordance with Only authorized service the terms of Active Life Return Material Authorization The manufacturer technicians may perform repairs adjustments or alterations to the instrument and its accessories Any violation voids the Precautions manufacturer s warranty 1 has the right to refuse to make repairs if the instruments or accessories are contaminated Do not use the instrument with incompatible equipment or with Authorized service technicians are trained and certified by the manufacturer accessories that are not authorized by Active Life Scientific Doing so may result in unreliable operation and may void certifications and warranties 2 Always observe proper shutdown procedure Failure to do so may result in corruption and or loss of data 3 The warranty becomes void and the manufacturer is not liable for direct or resulting damage 1f a the instrument or the accessories are improperly used prepared or maintained b the instructions in the User s Guide are not adhered to introduction G non authorized persons perform repairs adjustments or alterations to the instrument or accessories or d non authorized persons open the device 4 This instrument should only be used in compliance with its intended use Di With the exception of the Probe As
13. e Zoom out from a point bottom middle Use this to click a point you want to zoom out from f Zoom in from a point bottom right Use this to click a point you want to zoom in on 5 Back to Console Click Back to Console to go back to the Measurement Console screen 3 2 hardware After your sample is attached and positioned to its starting location and your Probe Assembly is properly attached see section 2 2 with a valid Probe ID Code entered in the software you may progress to section 3 2 1 3 2 1 Zero the Reference Force Display Ensure there is nothing on the Sample Plate before turning on the Measurement Stand Once turned on set the reference force scale to zero by pressing the TARE button on the right of the Reference Force Display It should be the last thing you do prior to taking a measurement 22 operating instructions 3 2 2 Prepare BioDent Hfc for Measurements Prepare BioDent Hfc for measurements by following Tuning instructions below l Ensure BioDent Hfc is in an Operable State Enable the Touchdown Distance TDD 1 Tuning Mode by clicking the Tuning Mode box below the Force Distance F D graph You will know the Tuning Mode is on when a check mark appears in the corresponding box This mode will allow you to physically adjust your TDD within the acceptable range of 50 250um Because Tuning Mode will be used for fine tuning your system and not for collecting data checking the box will preve
14. ed with Quick Study De Selected Protocol Displays the Measurement Protocol that will run when Measure is clicked If you selected O Quick Start while on the Study Information page this field is assigned with Temporary Protocol 3 Selected Sample Location Displays the Sample Location name that will be assigned to the next measurement If you selected D Quick Start while on the Study Information page no data is saved and this field is assigned with Quick Study Measurement Console Controls 1 18 Probe ID Code Displays the ID code you entered corresponding to the probe you will be using Measurement Console Measurements Remaining Displays the number of measurements remaining for the Probe ID code entered Reference Force Displays a range of acceptable Reference Force that should be exerted onto your sample via the Probe Assembly and Measurement Head Unit required in order to actuate a measurement at the specified force Measure Click O Measure to take a measurement using the selected Measurement Protocol x Cancel Click Xx Cancel to cancel a measurement in progress Measurement Console Tuning Mode Click the corresponding box to enable the Tuning Mode feature prior to each study and as necessary during your study Tuning Mode should be used when you are adjusting Touchdown Distance TDD within the acceptable 50 250um range See section 3 2 2 for TDD adjustment instructions operatin
15. ent raw data file format eee CSV text file tab delimited LTS BeCOM MC Cli iy asnsduicsnsccnencneedrcutacdemsvelevsidendsaddsbasaln 1 1 2 0 compliant Nework connectivity cccccccccssseesseseesseeeseeseeeeeeeeeee es Gigabit Ethernet port 54 MB s wireless Figures are approximate Actual performance during pre conditioning may vary 1 3 safety warnings and precautions Warnings L Do not use on live humans 2 The Probe Assembly probe used for indentation measurements is sharp and may be hazardous to the user Use extreme caution when handling using and disposing of probes To prevent accidental contact with the probe remove it when the instrument is not in use J This instrument is designed for use solely by researchers familiar with electromechanical laboratory instruments and with common laboratory safety procedures 4 Dangerous voltages are present inside the Measurement Stand chassis Attempting to open or service the instrument will void your warranty BioDent is the world s first commercially available Reference Point Indenter Y note BioDent is intended to be used for laboratory research and not used for diagnostic or therapeutic purpose BioDent is not intended for determining the safety and effectiveness of product s To ensure the safe operation of this instrument heed all warnings and follow instructions Ignoring warnings or using the product in a manner other than the recom
16. ent Protocol Setup To create a new protocol Click Create New and enter the following inputs accordingly a Protocol Name Give your protocol an easily identifiable name b Protocol Author s The names of those who created the protocol BioDent Measurement Protocol Setup C Notes Notes to help identify the intended use of this protocol e g if the protocol is ideal for testing bare bone no soft tissue d Pre Conditioning Settings Use pre conditioning settings if the surface of your test material is either not easily identifiable or extremely rough Pre conditioning parameters are approximate e Measurement Settings Enter a protocol that will produce data for later analysis Set the following inputs Indentation Force N and Indentations per Measurement Cycles To use a temporary protocol f Check the Temporary box to create a protocol that doesn t save to the Protocol Library This is a good option when developing a protocol you eventually want to save because it won t save drafted protocols along the way Other required inputs Navigation g After entering the protocol you want to use click C Next to proceed to Sample Planning or h Click Back to return to Study Information Bio ent Measurement Protocol Setup 3 1 3 Sample Planning k New Sample Location s Enter the name of the first sample you plan to measure and or the location you plan to measure on that same first
17. ep 6 note Attach and position your sample If you are using an Active Life approved Sample positioning is extremely important Adhere to instructions provided with your stage at all times Additional stage instruction and guidance documents are available at www activelifescientific com application stage consult the instructions provided with it to ensure correct sample attachment and positioning 11 getting started Q note It is highly recommended that you read and understand the Sample Positioning Stability and Probe Sample Guidance Document available at www activelifescientific com 12 Step 7 Use the following instructions to attach your Probe Assembly probe After completing this step return to section 3 1 5 Operating Instructions Measurement Console The Probe Assembly is sharp and may be hazardous to the user Use extreme caution when handling using and disposing of probes To prevent accidental contact with the probe remove it when the instrument is not in use square hub Test Probe Reference Probe Probe Assembly Carefully remove the Probe Assembly from its packaging and protective foam With the Test Probe and the Reference Probe coupled hold the Probe Assembly by the Reference Probe s square hub While holding the Probe Assembly by the square hub insert the top of the Test Probe into the opening of the Measurement Head Unit MHU there is an automatic magnetic attachment you
18. ference the position of measurements 4 5 control software 4 5 1 System is on but software doesn t recognize USB connection First verify the USB connection is made in the proper BioDent Hfc computer USB port indicated by a blue dot next to the correct port If it is unplug the USB connection and plug it back in Run a measurement If unable to try plugging the USB cord into a different USB port on the computer If unable to resolve contact Customer Care at customer care activelifescientific com for assistance 4 6 measurement stand 4 6 1 Measurement Stand does not appear to be turning on Check the fuses on the power input module If one is blown replace it with a fuse according to section 5 4 of this document For all other issues contact Customer Care at customer care activelifescientific com chapter 5 cleaning care and maintenance 5 1 life expectancy The standard warranty period for BioDent Hfc is one year The instrument s expected life is in excess of the warranty period under normal use and prescribed Care 5 2 periodic maintenance This product requires annual calibration by an authorized Active Life Scientific technician Between annual calibrations the unit should be frequently inspected for damaged cables to ensure proper operation It is recommended that the user inspect the Measurement Head Unit to ensure no debris is present in the Test Probe area and perform a Performance Check
19. g instructions Q note The Measurements Remaining field indicates how many measurements remain for the probe in use YQ note lf you cancel a measurement data for that measurement will not be saved and a measurement will not deduct from your Probe ID Code Q note lf TDD falls outside the acceptable range during a measurement you will be notified by a pop up and the measurement will been canceled resulting in no measurement data for that measurement Measurements are not deducted from the Probe ID count when measurements are automatically canceled operating instructions Inputs 1 Selected Protocol Click the down arrow on the Selected Protocol drop down menu to select a different Measurement Protocol from the Protocol Library 2 E Gear Symbol Click CA Gear Symbol to access the abbreviated Measurement Protocol Setup screen see section 3 1 2 to learn more about this screen 3 Selected Sample Location Click the down arrow on the drop down menu to load a previously saved Sample Location If a grey bar is shown over the down arrow it means there are no previously saved Sample Locations 4 Add Symbol If you want to add a Sample Location to your saved list enter it and click 5 Probe Identification Code The code you entered will be displayed To use a different one enter the new BioDent Hfc Probe ID Code 6 Quick Report Displays calculated data for the parameters selected in the Quic
20. h maximum load as specified by the user in the selected Measurement Protocol In the second third of the cycle BioDent Hfc holds the Test Probe in contact with the material at the maximum load In the final third of the cycle it retracts the Test Probe and attempts to reach zero load 6 7 calculated data Calculated Data are derived from the Converted Data and can be displayed after a measurement on the Measurement Console After completing a section of a study or an entire study you can save data to a Calculated Data file for analysis Calculated Parameters and associated units are listed below Abbreviation Name of sample being measured Measurement Number Indentation Distance Increase First Cycle Indentation Distance Creep Indentation Distance Total Indentation Distance Average Creep Indentation Distance Average Energy Dissipated Average Unloading Slope Average Loading Slope Average Maximum Force Touchdown Distance Notes Sample Location IDI 1st L ID 1st CID 1st TID 1st L Avg CID 1st L Avg ED 3rd L Avg US 1st L Avg LS 1st L Avg Max F 1st L TDD 1st
21. k Report Setup screen T Notes for By clicking on a measurement row in the Quick Report and then entering text in the Notes for box you can save notes for that particular measurement Notes will also be saved in the study s CalculatedData txt file 19 operating instructions Calculated Parameter Data l F D Graph Graphs BioDent Hfc measurement results on a Force Newtons Distance micrometers graph in real time The plotted data of the most recent measurement will remain on the graph until the Measure button is clicked again Quick Report To view other Calculated Parameter Data click a down arrow and select the output you wish to see Advanced Report Clicking Advanced Report takes you to a screen that allows you to review the data from previous measurements made in this study in detail including certain cycle by cycle results Navigation 1 hasurement Console Save Data Click O Save to save the Calculated Parameter Data from this study to a location of your choice e g on your desktop a network hard drive etc BioDent Hfc automatically saves a backup copy of the Raw Data Converted Data and Calculated Data to an internal directory but you can only access your data from the location you save the copy to Complete Study A pop up will appear after clicking Complete prompting you to verify that you want to exit the Measurement Console Click redirected
22. mended may place you and other parties at risk introduction oF Do not immerse the instrument in liquid or spray liquids directly onto the instrument 6 The instrument contains no user serviceable parts Removing the cover may introduce an electric shock hazard through exposure to high voltages and other risks If the instrument malfunctions do not attempt repair on your own Contact Customer Care at customer care activelifescientific com for instructions on how to return it to an authorized Active Life Scientific Service Center for service en i 7 Instrument installation shall be in accordance with the requirements of IEC 61010 1 Safety Requirements for Electrical Equipment for Damage to equipment or data is possible if instructions concerning proper use of the instrument and accessories are Measurement Control and Laboratory Use 8 The instrument is not suitable for use in the presence of flammable gases and should be located away from combustible materials not adhered to 9 Instrument grounding is vital for safe operation Plug power cord into a properly earthed main supply outlet with voltage and frequency characteristics that are compatible with those listed on the instrument and its corresponding manual 10 Do not use plug adapters or extension cords Such devices will defeat the safety ground and may cause injury Inspect the power cord and other cables routinely and discontinue use immediately if damage is
23. nt measurements from being deducted from your Probe ID Code Tuning Mode can also be used for training purposes but when enabled BioDent Hfc will not save or calculate any data from those a measurements 2 Position PMMA Place a block of PMMA on the Sample Plate then TARE the scale so it reads 0g J TDD Assessment First you will need to achieve a Reference Force BioDent h within the acceptable Reference Force range Take note of the Reference Force range displayed below the Measure button just above the F D graph Locate the large knobs towards the rear of the Measurement Stand and rotate them towards you to lower the MHU so the Probe Assembly makes contact with the PMMA Continue lowering the MHU until the Reference Force displayed on the Measurement Stand s blue Reference Force Display is within the range displayed on the Measurement Console screen Once satisfied click Measure Measurement Console CIS You will now use this first measurement to adjust your TDD As previously mentioned the acceptable TDD range is between 50 250um Observe the TDD achieved on the TDD 1 read out below the F D graph If your TDD is not within the acceptable range you will need to adjust it by rotating the black wheel that is between your Probe Assembly and the MHU 4 Adjust TDD When deciding which direction to rotate the wheel think of it as a clock that you are adding time to or taking time away f
24. ology BioDent Hfc 6 1 introduction BioDent Hfc consists of four primary components Measurement Head Unit MHU Measurement Stand Probe Assemblies and Workstation 6 1 1 Measurement Head Unit MHU The MHU consists of three major components force generator force sensor and distance sensor Components are assembled in a unique arrangement that eliminates the need for the rigid frame found in conventional mechanical testers 6 1 2 Measurement Stand The Measurement Stand delivered with your BioDent Hfc is specifically designed for bench top laboratory testing with several enabling features The MHU attachment mechanism enables optimal positioning of the MHU for short and tall samples Knobs on either side enable the smooth raising and lowering of the MHU on and off of samples Built in safety mechanisms exist to prevent MHU free fall in the event it slips off its tracks With a built in Reference Force scale the Reference Force Display enables the achievement of the desired Reference Force from measurement to measurement and study to study A removable Sample Plate facilitates attachment of Application Packages and Stages in varying sizes as well as the ability to clean the plate The Measurement Stand also consists of the Electronics Control which interprets BioDent Hfc electrical signals into Control Software information and vice versa 1 First the user selected Measurement Protocol is
25. ph curves and Calculated Data 6 2 why care about material properties Understanding how material properties influence a material performance is a fundamental aspect of assessing a material s ability to do its job It is becoming increasingly necessary for researchers in all fields to understand material level properties and how they contribute to a material s ability to be strong enough to bear load and serve its purpose in situ or in vivo 6 3 conventional methods Analytical instrumentation for measuring mechanical and individual material properties has existed for decades Traditionally mastered by mechanical engineers materials scientists or technicians these instruments typically require deep knowledge of the instrumentation and mechanical calculations Initially developed for the testing of inert materials such as plastics and metals these methods have recently been adapted to test biological materials However biological materials are complicated living and changing structures using conventional methods to measure their properties can be both a complex and intimidating endeavor 6 4 reference point indentation methods BioDent Hic Realizing the pressing need for material property testers specifically designed for Life Scientists BioDent Hfc was specifically designed for measuring biological materials at a Life Scientist user level Utilizing the unique features of Reference Point Indentation a technology invented by
26. rom however in this instance you re adding or taking away TDD If the TDD achieved is above 250m rotate the wheel counter clockwise in order to reduce TDD and vice versa To do this lift the MHU by using the large knobs make sure you get enough clearance from the PMMA to prevent any damage to the Probe Assembly Then calculate the approximate difference between the TDD achieved and the desired TDD One full revolution of the wheel is about 360m a good fact to keep in mind when deciding how much to rotate the wheel Rotate the wheel in whichever direction necessary After moving the PMMA over slightly to ensure you re measuring a new location lower the MHU back down by turning the large knobs toward you until the Probe Assembly makes contact with the PMMA and achieves proper Reference 2 40 Force The measurement and adjustment process is repeated until you achieve acceptable TDD 5 Disable Tuning Mode Once you finish adjusting TDD disable the Tuning Mode by clicking the corresponding box located below the F D graph You ll know it s disabled when the box no longer has a check mark in it 3 2 3 Prepare the MHU for your First Measurement Verify your measurement protocol on the Measurement Console before proceeding l Lower the MHU such that a You reach a reference force within the Reference Force range b Your probe makes good solid and stable contact with the measurement location of your sample This must
27. sembly and application stages do not expose the instrument to moisture or operate in wet areas 6 Ensure the power supplied to the instrument matches the power requirements printed on the rear of the instrument near the power inlet module T For continued protection against risk of fire replace fuses with T1 0A 250 V type only 8 Do not excessively bend or kink the instrument power cord or connecting cables 9 Handle the instrument carefully as it is a calibrated high precision instrument If the instrument is dropped or otherwise damaged contact Active Life Scientific immediately 10 Handle the probe with caution The probe features a small diameter shaft which if bent may result in inconsistent measurements Inspect the probe before each use for damage If probe is bent or otherwise damaged or if probe is expired discard the probe 11 The instrument includes a protection fuse at the power entry point If the Q nole fuse fails it may be removed and replaced with a new one If the replaced See section 5 4 for information on fuse fails the instrument must be serviced by an authorized Active Life fuse replacement Scientific technician Failure to observe safety warnings and precautions contained herein may result in injury to the user or third parties and may invalidate the instrument warranty 1 4 confidential information and computer network safety Information entered into BioDent Hfc can be accessed by o
28. sent through the Workstation to the MHU force generator 2 A voltage signal is then sent from both the MHU internal force and distance sensors through the Workstation to the Control Software 3 Voltage signals are paired according to time and saved in Raw Data Files 5 This time paired voltage data is converted to Newtons force units from the force measurer and micrometers distance units from the distance measurer and saved into the Converted Data Files 6 The time paired force and distance data is then graphically displayed in the Control Software in the form of Force Distance F D Force Time and Distance Time graph curves 7 These graphs are processed to calculate a variety of parameters Calculated Data 6 1 3 Probe Assemblies Probe Assemblies consist of two components Reference Probe and Test Probe Probe Assemblies are the interface between your samples and BioDent Hfc When using a Probe Assembly with a Reference Probe the Reference Probe establishes a Reference Point for the distance measurement usually relative to the surface of a hard material while the Test Probe is actuated into and out of the sample from a position relative to that Reference Point This is completed without the need of a rigid frame 6 1 4 Workstation The Workstation consists of the BioDent Hfc Control Software The Control Soft ware is where you the user tell BioDent Hfc what Measurement Protocol to use and review F D Gra
29. thers Protecting data and other confidential information is your responsibility and not that of BioDent Hfc or Active Life Scientific introduction 1 5 symbols and abbreviations Reference the guide below to learn what each symbol in this User s Guide and on your product means The safety of the user or third party is at risk Follow all product warnings and instructions for safe operation Refer to User s Guide instructions for proper operation of the instrument Hazardous voltages are present in the device Manufacturer information Date of manufacture of the device 3 EP P chapter 2 getting started Refer to the BioDent Hfc Setup Guide for proper setup procedure Once complete proceed below to ensure you have everything you need to begin taking measurements 2 1 supplies 2 1 1 Required Biodent 1 BioDent Hfc 2 Approved Active Life 3 Approved Active Life 4 Test sample or material Probe Assembly Reference Material 2 1 2 Recommended Application Stage Using the proper application stage to achieve optimal sample positioning and stability is extremely important We recommend an approved Active Life application stage to hold and position your materials Pictured below is the Ex Vivo Small Bone Stage available for testing ex vivo small rodent bones Probe Assembly Cleaning Supplies For recommended Probe Assembly cleaning supplies see instructions for Probe Assembly Care available at www
30. to the Study Information screen Click to stay on the to exit the Measurement Console screen you will be Measurement Console screen BioDent h operating instructions 3 1 6 Advanced Report 1 Select Measurement Click the Select Measurement down arrow to select a measurement from your study to review in more detail 2 Select Graph to Display Click the Select Graph to Display down arrow to view other calculated parameter data for the selected measurement Advanced Report CE hack E Cor Advanced Report hat 3 Graph Manipulation Tools Tools to view graphs in different ways a Cursor movement tool left Moves the cursor on the display b Zoom middle Provides in out zoom options on the display C Panning tool right Grabs the plot and moves it around the display Advanced Report 3a 3b 3c Pa D aml Enlarged view of Graph Manipulation Tools 4 Zoom Options a Zoom to rectangle top left Use this to draw a box around an area on the graph that you would like to zoom in on b X zoom top middle Use this to zoom in on an area of the graph along the x axis C Y zoom top right Use this to zoom in on an area of the graph along the y axis 21 operating instructions vanced Report A a 4b 4c pe 4d 4e 4f Enlarged view of Zoom Options d Zoom to fit bottom left Use this to return to the original view BioDent Hfc showed you
31. will feel if inserted correctly Hold the black wheel still with your free hand As you push the Reference Probe up ensure the Reference Probe engages the thread Once it does gently twist it counter clockwise until finger tight 13 chapter 3 operating instructions 3 1 BioDent Hfc control software 3 1 1 Study Information Study Information Study Information 14 To continue a study or to access data from a previous or ongoing study 1 Select the study from the Previous or Ongoing Studies drop down menu on the left side of the screen 2 Click gt Wext to advance to the Measurement Console section 3 1 5 To start a new study 1 Enter the name of your study 2 Indicate who will be conducting the study 3 Include a description of your study optional 4 Click gt Wext to advance to the Measurement Protocol Setup screen To get started quickly if you don t have a specific study in mind or simply want to explore protocol ideas before saving them to the Protocol Library 1 Click gt Quick Start to advance to the Measurement Console section 3 1 5 To exit the program 2 Click Exit Program to exit the program What kind of study are you running Clicking D opens the Control Software in Quick Study mode and disables the ability to save study settings and measurement data Keep the Quick Study mode open to review data in Quick and Advanced
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