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1. 000 with 2 L bottles Diluent autonomy 63 cycles with 2 L bottles Circle mode patented 3 dilutions on a 90 mm Petri dish 6 dilutions on a 150 mm Petri dish Stylus disinfection system patented inside and outside by Overflow technology Pressure in the stylus 8 bars Successive plating capacity with the same sample up to 20 Petri dishes 50 uL Traceability excel M datamatrix labels Monthly cleaning program v Filling time programmable via USB v My Diluent key v Sample mixing before dilution plating v Controlled by microprocessor v Dimensions w x d x h 39 x 42 x 29 cm Weight 15 6 kg Power supply 100 240 V 50 Hz to 60 Hz Power 65 W 1 year guarantee after registration v All stainless steel v Made in France v V08 050 V08 034 7218 4833 2 15189 DABAM AOAC ISO WEEE RoHS Joal Andy 977 97 saa 2002 96 EC 2002 95 EC Interscience INTERNATIONAL 30 ch Bois Arpents 78860 St Nom FRANCE T 33 0 1 34 62 62 61 F 33 0 1 34 62 43 03 info interscience fr www interscience fr Interscience USA amp CANADA 199 Weymouth ST ROCKLAND MA 02870 USA T 1 781 792 2133 F 1 781 792 2134 info intersciencelab com www intersciencelabo com InNcerscience CHINA JETT ASL K R49 277 5 8 2B AJB 7182 2000380 FA 15 86 0 21 64739390 FAL 86 189 3097 0733 sales china interscience fr www interscience fr chin
2. a Distributor www INGErTSCIENCE Fr easySpiral Dilute_B_0615 Pictures and information in this brochure are not contractually binding Spiral easySpiral easySpiral Dilute Scan are trademarks This document is the translation of the french brochure and is not contractually binding INTERSCIENCE reserves the right to change or improve the specifications of its products without notice Please visit www interscience fr for current updates and additional information RCS 950 356 220 Versailles INTERSCIENCE SARL F78860 eN
3. e Circle mode 3 or 6 surface dilutions on 1 Petri dish 10 uL inner circle 16 uL middle circle and 25 uL outer circle concentrated concentrated Counting on Petri dish up to 1 x 10 2 CFU mL fa 107 107 10 Savings on Petri dishes Pour mode Homogenous deposition in pour plate mode 50 uL 100 uL 200 uL and USB programmable Homogenous deposition in pour plate mode Easy colony counting for invasive colonies Pharmacopoeia compliant In pour mode easySpiral Dilute deposits one sample drop at the bottom of an empty Petri dish Then the user adds the molten agar on top and homogenizes it with the sample How to count Manual colony counting R Scan 100 Po ry with Spiral counting grid or Automatic colony counting Scan 300 Scan 500 Scan 1200 Scan 4000 Questions What is special about the easySpiral Dilute easySpiral Dilute is the only 2 in 1 equipment on the market making automatic serial dilu tions and standardized plating The accuracy of easySpiral Dilute is high 0 5 How can I check it usually dilute 1 mL to 1 10 and the easySpiral Dilute uses 0 5 mL to 1 10 Is easySpiral Dilute as precise with a volume divided by 2 It is easy and automatic Test of the deposited volume during plating Press on the plating volume test button This function will fill a previously weighed beaker with 500
4. easySpiral Dilute The world s first automatic diluter amp plater Li A revolution o E IN serial dilutions Interscience Interscience Our quality for your lalo e Designer and manufacturer for microbiology e R amp D leadership for innovative and reliable products er interscience e Worldwide distribution network in more than 85 countries R amp D center and manufacturing plant Mourjou FRANCE e Made in France Spiral method 35 years experience SPIRAL The Spirale method was designed in 1973 to automate the routine work of bacterial enumeration by Dr Ed Campbell researcher at the FDA Food amp Drug Administration With Fran ois Jalenques friend and founder of interscience they patented an updated method in 1992 Since then Spirale automatic platers have been a reference for applications in food microbiology medical bacteriology research on food preservatives or cosmetological factors in compliance with the NF VO8 050 NF V0O8 034 ISO 7218 ISO 4833 2 ISO 15189 FDA BAM and AOAC 977 27 standards Today interscience is proud to launch the 4 generation of Spiral platers with easySpiral Dilute the world s first automatic diluter amp plater F Jalenques amp Dr Ed Campbell S h i 4 spits IN a i gt J Spiral DS e ER interse 1 eohi i P eesySpirali z5 First Spiral plater a d i 1996 Spiral DS i nreregenc Automat
5. h a new automatic dilution sequence Accessories amp technical specifications i Link E wi dataLink Traceability system Housing protection Transparent housing for working outside laminar flow Ref 413 001 Ref 410 100 T 1 1 l Beakers DB 50 1 000 sterile beakers for Spiral platers 5 mL Barcode reader Barcode reader for traceability Ref 415 100 Ref 522 000 easySpiral Dilute is delivered with 1 000 sterile beakers 1 blue dye for tests 1 box of EnzyClear 4 sets of GL 45 bottle connectors Petri dish rings 55 90 amp 150 mm Spiral counting grids 90 amp 150 mm circle counting grids 90 amp 150 mm power cord user s manual monitoring CD ROM software USB cable easySpiral Dilute Reference 414 000 Petri dish diameter 55 mm 90 mm and 150 mm Syringe capacity 1 000 uL Programmable dispensed volume via USB from 10 uL to 1 000 uL Preset dispensed volume 50 uL 100 uL and 200 uL Detection in CFU mL of Spiral plating 30 to 1 x 10 CFU mL Plating modes 55 mm constant pour plate 90 mm exponential 10 constant circle 3 dilutions pour plate 150 mm exponential 10 constant circle 6 dilutions pour plate Number of dilutions at 1 10 for 1 cycle 5 1 disinfection 5 dilutions 1 plating of 1 dilution 134 s 1 disinfection 5 dilutions 1 plating of each dilution 234 S Disinfectant autonomy 1
6. ic Spiral plater 7 2010 easySpiral World s fastest Spiral plater PATENTED 2012 easySpiral Dilute World s first automatic diluter amp plater PATENTED SYSTEMS interscience is proud to introduce easySpiral Dilute a 2 in 1 automatic diluter and plater which allows you to do 5 x 1 10 serial dilutions It will then automatically plate on 1 single Petri dish with a countable range from 30 to 1 x 10 countable CFU mL No more manual dilutions and plating is automatic gt Place sample gt Place your sample in the beaker 1 The easySpiral Dilute will automatically dilute the sample up to 5 times 2 The easySpiral Dilute will automatically plate the sample on a Petri dish up to 1 x 10 countable CFU mL Why use an automatic diluter amp plater easySpiral Dilute allows you to save 50 on consumables and 50 on your time Comparison between manual and automatic method below Example of 50 samples Dilution 107 Plating 10 10 and 10 Manual methoq CONSUMABLES Dilution e 400 tubes Plating e 450 Petri dishes SSS TIME O Automatic method CONSUMABLES Dilution e 350 beakers Plating e 150 Petri dishes TIME Key features Compact High precision glass syringe 38 cm wide can be used inside and outside a laminar flow Typical accuracy of 0 5 Stainless steel housing 4x more precise than a basic pipet No more pipet calibrat
7. ion High speed automation Fast rotating arm 1 disinfection 5 dilutions and 1 plating in 134 s PATENTED interscience easySpiraliS Dilute Spiral Automatic disinfection 3 One touch plating Overflow technology cleaning system Choose from 4 plating modes No cross contamination 5 Plate on 55 90 and 150 mm Petri dishes PATENTED Easy serial dilutions High capacity Choose your dilution factor 2 fully autoclavable bottles Do 10 dilution or more automatically disinfectant amp diluent and connectors No more 9 mL tube preparation PATENTED How does it work 1 Dilution cycle a 2 3 1 2 sample sample w sample w sample W 10 l 10 10 sample a 10 Dilution cycle can be repeated as many times as you wish Sample intake 0 5 mL of sample is deposited 4 5 mL of diluent is added to the sample and mixed The above dilution cycle is repeated for the next serial dilution 0 5 mL of 10 diluted sample intake 0 5 mL of 10 diluted sample is deposited 2 Plating cycle Constant mode Homogenous surface deposition 50 uL 100 uL 200 uL and USB programmable Examples Before incubation Homogenous surface deposition After incubation Easy colony counting No colonies on the edge of the Petri dish Exponential mode Decreasing surface concentration 50 uL 100 uL 200 uL and USB programmabl
8. uL Weigh the distributed volume to check the results Test of the volume of the dilutions Press on the dilution volumes test button This function will fill 2 previously weighed beakers one with 0 5 mL and the 2 one with 4 5 mL Weigh the distributed volumes to check the results Which diluents can I use easySpiral Dilute works with saline solu tions e g Ringer s solution physiological saline Avoid using diluents with nutrients as they may cause contaminations Bacteria only remain a few seconds in the beakers before being de posited on the Petri dish so using a saline dilu ent has no effect on the final enumeration It is however possible to use nutrient solutions as diluent in the semi automatic My Diluent mode In that case fill in the beakers with 4 5 mL diluent before processing Usually the average accuracy for the 9 mL tubes filling and 1 mL pipetting is 2 with the need to calibrate and regularly check the pi pets you are using easySpiral Dilute has an average accuracy of 0 5 on 0 5 mL and 4 5 mL volumes because it works with a high precision Hamilton glass syringe in a liquid displacement circuit With volumes divided by 2 precision and repeatability are then much higher need to make a 10 dilution How can do it The easySpiral Dilute can do as many dilutions as you wish Do a 10 dilution take the last beaker and place it as the mother sample and then proceed wit

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