User Manual Lx 300 - Labo America, Inc.
Contents
1. Fig 20 Placing specimen on the stage 1 1 Rotate the coarse adjustment knob 2 in anticlockwise direction to fully lower the stage 2 2 Open the bow shaped lever 3 outward by pulling on lever handle 1 place the specimen by sliding the specimen glass R plate s on the stage from the front toward the slide seat at the rear EE 5 3 After positioning your specimen slides 2 max return the bow shapedlever 3 gently by slowly releasing control knob 1 4 Rotating the upper co axial knob controlling the Y axis movement 4 moves the specimen in the vertical direction Rotating the lower knob 5 moves the specimen in the X axis or horizontal direction Do not adjust the specimen holder directly by hand for this will damage the rotary mechanisms When the specimen holder reaches the stop position the rotation force of the X Y knobs become stiff Stop rotating the knob at this point Fig 21 e a Lx 300 Cover glass Slide glass Fig 22 Cover glass This is the glass plate placed on the specimen For optimum optical performance t2. The inter pupillary distance adjustment consists of regulating the two eyepieces to align with both eyes pupils so that you can observe a single microscopic image through two eyepieces in stereo vision This greatly helps to reduce fatigue and discomfort during observation While looking through the eyepieces move both eyepieces laterally until the left and right fields of view coincide completely The position of index dot indicates the inter pupiliary distance value Note your interpupillary distance so that it can be quickly referred to in the future This is happen when multiple users work with the microscope Siedentopf binocular head 45 inclined IPD 45 75 mm is designed with a special feature to adjust viewing height up to 40 mm as per user s convenient and comfort Refer fig 26 for better understanding Procedure for adjusting the diopter 1 Rotate the right eyepiece to match the markings of your IPD If your IPD is 64 rotate the eyepiece to 64 mark 2 While looking through the right eyepiece with your right eye rotate the coarse and fine adjustment knobs to bring the specimen into focus 3 While looking through the left eyepiece with your left eye rotate only diopter adjustment ring on the eyepiece until specimen is at its best possible focus Using the Eye Guards When Wearing Eyeglasses Use with the eye guards in the normal folded down position This will prevent the eyeglasses fro
3. by agitation Pg 20 The specified immersion oil is not used Use the immersion oil supplied by LABOMED 5 Part of image is defocused The objective is not properly engaged in the light path Engage the objective into position until the nose turret clicks The specimen is not set properly on the stage Set the specimen correctly on the stage and secure using the specimen holder Pg 16 6 Coarse focus adjustment cannot lower the stage low enough The condenser is too low Raise the condenser 7 Fields of view through both eyepieces is inconsistent The interpupillary distance is not adjusted properly Dioptric compensation for the two eyes is not set The left and right eyepieces are of different magnification Adjust IPD to the appropriate setting Pg 17 Adjust diopter settings Pg 17 Ensure that both eyepieces are of are of same magnification LABOMED does not recommend using third party eyepieces in conjunction with LABOMED eyepieces 21 Lx 300 Observation Cause Remedy 8 Objective hits the specimen when an objective is switched to a higher magnification objective 9 Bulb LED does not turn On 10 Bulb LED blows easily The specimen slide is upside down Set the specimen correctly with the cover glass facing upwards The cover glass is too thick Use a cover glass with thickness of 0 17mm The stage is raised too high
4. net 8 Electrical Halogen 6V 20W Charging time Upto 2 000 hours LED Battery 7 4V 1000mA Charging time Upto 5 hours with totally consumed battery Total battery time Upto 4 hours 9 Operating environment Indoor use Altitude Max 2000 meters Ambient temperature 5 to 40 C 41 to 104 F Maximum relative humidity 80 for temperature up to 31 C 88 F decreasing linearly through 70 at 34 C 93 F to 50 relative humidity at 40 C 104 F Supply voltage fluctuations Not to exceed 10 of the normal voltage Pollution degree 2 in accordance with IEC60664 Installation Overvoltage category II in accordance with IEC60664 23 SS Urr e www laboamerica com Our policy is one of continuous development Labo America Inc reserves the right to change design and specifications without prior notice Labo America Inc U S A 920 Auburn Court Telephone 510 445 1257 Fremont Fax 510 991 9862 CA 94538 sales laboamerica com Ku LABOMED and Lx 300 are registered trademarks of Labo America Inc Us SJ With a policy of continuous development Labo America Inc reserves the right to change design and specifications without prior notice STERY 6 2900 I ahn Amarica Ine O1260N0N OONA Mf2 20900 Printed in U S A
5. 18 1 Unscrew two screw shown as 1 and take the complete battery cartridge 2 Again unscrew two screws shown as 2 and remove carrier 3 3 Replace battery shown as 4 by a new one in the right carrier 3 with the help of screws 2 4 Refit the cartridge by screwing both screws shown as 1 Fig 18 14 Lx 300 Engage the 10X objective in the light path l Bring the specimen in focus Adjust the observation tube and eyepieces Adjust the interpupillary distance Adjust the dioptric setting Adjust the aperture of iris diaphragm Engage the objective to be used in the light path and bring the specimen in focus Engage the required filters Adjust the brightness Observe Specimen 15 Flip the main switch ON m Place the specimen on the stage L g J e Fuse Holder Main switch Lx 300 1 DETAILED OBSERVATION PROCEDURE Turning the Lamp ON 1 Flip the main switch to T ON as shown Figure 19 2 Rotating the light intensity adjustment knob Fig 20 in the direction of the arrow increases brightness and rotating knob in the opposite direction decreases brightness The intensity bar next to the knob indicates the direction of intensity level
6. Lower the stage The slide has slipped from the slide holder Slide is of excessive thickness Bulb LED is not mounted Re position the slide in the slide holder Use slides with thickness between 0 9 and 1 4mm Attach a Bulb LED Bulb LED is blown Replace the Bulb LED The power cord is unplugged Not firmly secured Ensure power cord is securilly plugged into the box socket wall outlet Fuse is blown Check and replace with live fuse Battery is low The specified Bulb LED is notused Charge battery Replace with the specified Bulb LED Lx 300 1 SPECIFICATIONS 1 Illumination Built in illumination system Halogen Bulb LED 2 Focusing mechanism Stage height adjustment mechanism Fine adjustment scale 3 0um per graduation Fine adjustment stroke 0 3mm per turn Total stroke 12 7mm Co axial coarse and fine focusing on ball drive 3 Revolving nosepiece Quadruple positions fixed Reverse angle 4 Observation tube Binocular Field number 20 mm Standard Tube tilting angle 45 Interpupillary distance 48 75 adjustment range 5 Stage Size 200 x 160mm with mechanical stage Movement range 78 x 54mm Specimen holder Double specimen 6 Condenser Type Abbe condenser daylight filter detachable N A 1 25 Aperture iris diaphragm Built in 7 Dimensions amp Weight 284 0mm L x 227 0mm W x 360 6mm H 7 kg
7. P LABOMED To ensure proper use of this instrument as well as to avoid injury while operating instrument understanding this manual completely before use is highly recommended E C G O INTRODUCTION The Lx 300 is a clinical microscope reflecting a modern design as well as the latest in optical and mechanical advancements Designed for professionals as well as clinicians this microscope offers many features and functions with excellent ergonomics fora fatigue free usage Here are few points highlighting the benefits of the Lx300 Extra clarity and contrast is provided through a 360 rotatable Binocular body inclined at 45 with IPD and height adjustment The pressure die cast stand consists of ball bearing frictionless sideways focusing to avoid any loss in motion All painted surfaces are with anti microbial properties The sturdy new stylish design provides a high degree of comfort as well as stability The high powered objectives are spring loaded to prevent accidental damage to specimen slides The quadruple nosepiece has a comfortable ribbed grip for easy rotation that also safeguards the turret system against any damage All positions are par centered and par focalised ensuring the highest level of accuracy The ball bearing mechanical stage allows smooth travel over a 78 x 54mm area with spring loaded stage clamps for retaining the specimen at the e
8. e a solution of diluted hand soap liquid initially If this does not produce a satisfactory result repeat the cleaning using a solvent Optical Cleaning Solution 85 petroleum ether and 15 isopropanol 6 Grease must always be removed using a solvent 7 Cleaning is achieved by using a spiral motion from the center to the rim Never wipe using zig zag movements as this will only spread the dirt With larger optical surfaces e g tube lenses the spiral motion starts initially at the rim before moving to the middle and is then followed by a center to rim cleaning motion Normally several spiral wipes are recommended We recommend pure volatile petroleum ether or Optical Cleaning Solution as explained in point 3 above zig zag motion X spiral motion v Wipe using a spiral movement Do not use a zig zag motion 2 Cleaning of painted surfaces Avoid the use of any organic solvent e g thinner xylene ether alcohol etc for cleaning of painted surfaces of the instrument Painted surfaces can be cleaned with a very lightly moistened micro fiber cloth Loose dust and other dirt particles can be removed using a soft bristle brush used exclusively for this purpose Lx 300 Lx 300 Binocular Eyepieces Binocular viewing tube 45 inclined Revolving nosepiece Objectives Specimen holder Mechanical stage Abbe condenser Filter holder Coarse and fine focus knob On off intensity regulator X Y moveme
9. e touched unless system has completely cooled down Before use carefully read the instruction manual Improper use could result in injury to the user and or damage to the equipment Warning against risk of electric shock Main switch is ON O EPE Main switch is OFF Caution If the microscope is used in a manner not specified by this manual the safety of the user may not be warranted In addition the equipment may also suffer damage Always use the equipments as outlined in this instruction manual os aaa 2 Lx 300 Getting Started 1 A microscope is a precision instrument with delicate glass components Please handle with care 2 Do not use the microscope where it is subjected to direct sunlight high temperature humidity dust and vibrations For the operating conditions see chapter 14 SPECIFICATIONS 3 The microscope is ventilated by natural convection Be sure to leave enough space 10 cm or more around body when installing the unit 4 Arm handle is provided for carrying the microscope To prevent damage do not hold the microscope by the stage or observation tube Be sure to remove the specimen from the stage clip while transporting unit to avoid damage to the Fig 2 specimen slide Maintenance and Storage 3 1 Clean all glass components by wiping gently with cleaning cloth provided To remove fingerprints or oil smudges wipe
10. ethylene bag so as not to taint the bulb with fingerprints and push the bulb into the pin holes on the socket 3 After attaching remove the polyethylene bag 3 With the lock knob pulled out close the lamp housing door then push the lock knob back to lock the cover Always use the designated bulb Using a bulb other than those specified by LABOMED may lead to a fire hazard Fingerprints or stains on the lamp bulb reduce its life If contamination occurs wipe bulb surface with a cloth slightly moistened with alcohol Caution For Bulb Replacement During Use or Right After Use The bulb lamp socket and areas near these will be extremely hot during and right after use Set the main switch to O OFF disconnect the power cord from the wall outlet and allow the bulb and lamp socket to cool before replacing the bulb with a new bulb of the designated type Cooling time may vary depending on condition of use Procedure for LED replacement figure 14 amp 15 1 Lay the microscope on a clean surface toward right so that the microscope base is exposed 2 Using a screw driver unscrew four screws 1 to open the base of the microscope 3 Remove three screws 2 provided on the lamp housing use screw driver to open the lamp housing 4 Open the LED mount by opening the two screws 3 5 Replace existing LED mount with live LED mount 6 Reverse the steps 4 to 1 to complete the process Appl
11. eview the following list and take corrective action as needed If problem persists please contact LABOMED or your local LABOMED dealer Observation Cause Remedy 1 Uneven brightness in observation field The objective is not engaged in the light path The condenser is too low Engage the objective into position until the nose turret clicks Raise up to achieve more light Pg 18 The objective eyepiece condenser and or window lens are dirty Clean them thoroughly as previously prescribed in Optical Cleaning Pg 4 2 Dust or stains are visible in observation field The eyepiece condenser window lens and or specimen glass is dirty Clean glass parts thoroughly with lens tissue and cleaning solution prescribed in Optical Cleaning Pg 4 3 Glare visible in field of View The condenser is too low The condenser iris diaphragm ring is closed Raise condenser light Adjust the aperture according to the objective magnification Pg 19 4 Observation image is hazy or unclear The objective is not engaged in the light path Engage the objective into position until it clicks Pg 19 The objective eyepiece condenser and or specimen glass is dirty Clean glass parts thoroughly with lens tissue and cleaning cloth Immersion oil is not used with an immersion objective Use immersion oil as suggested Pg 20 Bubbles are presentin immersion oil Remove the bubbles
12. he cover glass thickness which is the distance from its surface to the specimen surface should be 0 17 mm Slide glass This glass plate should ideally have a length of 76 mm width of 26 mm 1 mm and thickness between 0 9 and 1 4 mm Vernier Scales These scales allow for easy identification of the specimen s position co ordinates making it easy to return to a particular region of interest after scanning the slide Figure 23 1 The horizontal co ordinate can be read at position 1 on the specimen holder 2 The vertical co ordinate can be read at the index line 2 Focusing Procedure Figure 24 1 Rotate the coarse adjustment knob 1 clockwise so that the objective 3 is as close as possible to the specimen We recommend starting with 10X 2 While observing the specimen through the eyepieces slowly rotate the coarse adjustment knob 1 counterclockwise to lower the stage 3 When coarse focusing of the specimen is obtained an image is observed rotate the fine adjustment knob 2 for fine detail focusing Working Distance WD The WD refers to the distance between each objective and the specimen when acute focus of the specimen is obtained Objective Magnification 4X 10X 40X 100X WD mm 30 5 4 82 0 55 0 11 18 8 Lx 300 Adjusting the Interpupillary Distance IPD 437 5 389 4 Fig 26
13. icable Bulb LED replacement 6V20W Halogen bulb P N CX 013 6V30W or LED P N 9135000 901 Lx 300 Mounting the Daylight Blue Filter I 7 This filter modifies the color of observation light into a natural daylight gt color 2 O g Fit the filter 1 into the bottom of the condenser 2 until it clicks into f Figure 16 1 E amp E K Fig 16 Installing or Replacing the Fuse Before replacing the fuse remove the parts that may drop such as the filter and specimen from the microscope frame Turn around the microscope to its back so that the AC inletis visible figure 17 1 Usea flat head screw driver to open the fuse holder 1 2 The fuse tray will come out with 2 live fuse and 3 spare fuse Do not pull out the fuse tray with force as it is locked and will not be out completely 3 Replace the primary fuse 2 with the spare fuse 4 Engage the fuse tray back in Always use the designated Fuse Using a fuse other than those specified by LABOMED may lead to afire hazard Caution For Fuse replacement Set the main switch to O OFF disconnect the power cord from the wall outlet Installing or Replacing the Battery Before replacing the battery lay down the microscope as shown figure
14. m being scratched When Not Wearing Eyeglasses Extend the folded eye guards outwards direction of the arrow to prevent ambient light from entering into your line of vision 17 Lx 300 Centering the Condenser The condenser is fixed and cannot de center In case of any error remove the condenser by loosening thumb screw and re install the same Fig 29 a Adjusting the Condenser Position and Aperture Iris Diaphragm The condenser is most often used in the highest position If the observed field of view is not bright enough brightness may be improved by lowering the condenser slightly 1 Rotate the condenser height adjustment knob 2 to move the condenser to the highest or desired position 2 The aperture iris diaphragm ring 1 has an objective magnification scale 4X 10X 40X 100X Slide the diaphragm lever left right to achieve the desired illumination level Rotate the revolving nosepiece so that the objective to be used is in line above the specimen Always use the ribbed grip 1 to rotate the objective nosepiece Fig 31 19 Lx 300 Using the 100X Immersion Objective Fig 32 The designated immersion oil should be in contact with the cover le
15. ns of the 100X immersion objective If not the specimen will appear distorted and dull Itis recommended that LABOMED immersion oil is always used Immersion Process 1 Bring the specimen in focus using first the 10x then 40x objective 2 Disengage the 40x cycling towards 100x and place a drop ofimmersion oil on the center point of the specimen 3 Rotate the revolving nosepiece to engage the immersion objective and rotate the fine adjustment knob to bring the specimen into focus Since air bubbles in the oil will affect the image quality make sure that the oil Is free of bubbles To remove bubbles rotate the revolving nosepiece slightly to agitate the oil 4 The condenser of this microscope manifests the full performance when oilis placed between the slide glass and the frontlens of condenser If oil is not placed there the observed image may appear dark 5 After use remove oil from the objective front lens by wiping with lens tissue slightly moistened with an ether 70 alcohol 30 mixture Caution If immersion oil makes contact with your eyes rinse eyes out thoroughly with fresh water If immersion oil makes contact with skin wash affected areas with soap and water If prolonged discomfort is experienced consult your physician immediately s TROUBLESHOOTING GUIDE Lx 300 Under certain conditions performance of the unit may be adversely affected by factors other than defects If problems occur please r
16. nt control knob ed Sage E H Ee of E G LA v Microscope Arm 7 Lx 300 STANDARD COMPONENTS After removing your microscope from its packaging make sure that all of the following contents are present Please note that the contents of your microscope may vary as the optional configuration contrasting method or viewing body opted for may not be of the standard configuration highlighted here Eyepieces fixed at 10x A gt Binocular observation tube Allen Screw Objectives Stage 2 Microscope Arm a e U E Daylight blue filter Paired Eyepieces Allen Wrench 3mm V Power Cord 6V 20W Halogen bulb Lx 300 OPTIONAL ACCESSORIES System Diagram of Optional Accessories Binocular head WF 10x L a a ee E sH 4x F 10x S 40x SP ee SP Poit SP PH 20x SPPH an SL SPPH 100x SL Oil q o i 90 o s U g Abbe Condenser 1 25 Polarizing kit Phase turret Blue filter Green filter J lt a qQ a E Banc Bulb LEQ Mount sia coasts Battery Lx 300 Installation and Operation of Optional Accessories Phase Turret K 1 Remove the condenser from its position b
17. ol before touching Fig 1 Fig 1 Applicable bulb LED replacement 6V20W Halogen bulb P N CX 013 or LED P N 9135000 901 3 Install microscope on a sturdy level table or bench and avoid any restriction of air vents in the base of the unit Do not place microscope on a flexible surface as this could result in blocking the air vents and cause overheating 4 Always use the power cord provided by LABOMED If the proper power cord is not used product safety performance cannot be warranted 5 When installing the microscope route the power cord away from the microscope frame Should the power cord come in contact with the microscopes base the power cord could melt due to overexposure to heat 6 Always ensure that the grounding terminal of the microscope and that of the wall outlet are properly connected If the unit is not grounded LABOMED can not warrant electrical safety 7 Never allow metallic objects to penetrate the air vents of the microscope frame as this could result in user injury and damage to the microscope 8 After operation of microscope be sure to disconnect power cord from connector socket of the microscope or from the wall power outlet Safety Symbols The following symbols are found on the microscope For optimal use it is recommended that users understand these symbols and always use the equipment as prescribed Symbol Explanation This surface has a tendency to heat up and should not b
18. r local rules regulation for product disposal Lx 300 Care amp Maintenance Your microscope has been engineered for a long and safe operational life with the least amount of maintenance required In general routine maintenance is limited to keeping the microscopes working parts lubricated and optics clean Always cover the microscope with the provided dust cover when notin use Optical Cleaning 1 The objectives have been adjusted for a tight fit to prevent any damage during transportation To remove an objective rotate it counterclockwise while gripping it with a rubber sheet etc to avoid any slippage 2 To clean the lens surfaces remove dust using a soft brush or compressed air cans available at your local electronics store For removing finger marks or grease soft cleaning cloth or lens tissue lightly moistened with cleaning solution 85 petroleum ether and 15 isopropanol should be used For cleaning the objective optics use Methanol Observe sufficient caution in handling Methanol Place the objectives and or eyepieces on a dust free surface e g aluminum foil All other optical components to be cleaned should be as accessible as possible 3 Blowall loose dust particles away with compressed air or mini dust blower Remove all water soluble dirt with distilled water If this is unsuccessful repeat using a solution of diluted hand soap liquid Remove any remaining residue with a dry cotton swab 5 To remove oil us
19. wise while holding it with a rubber grip to avoid any slippage Install the observation head using the following procedure 1 Using allen wrench 3mm provided loosen the Head Locking Screw 1 and remove the dust cover cap provided in dovetail cavity as well as on observation head dovetail 2 Mount the Observation Head by engaging the dovetail provided at the bottom of the head into the dovetail cavity provided in the microscopes arm 3 Tighten the Head Locking Screw 1 after positioning the Observation Head as desired See figure 11 Insert the eyepieces into the ocular tube of Observation Head using following procedure 1 Remove the protective caps from the observation tube 2 Insert 10x eyepieces into the ocular sleeve and tighten the clamping screw 1 using allen wrench 1 2mm See figure 12 Fig 12 12 Lx 300 Your Lx 300 can be easily assembled by simply attaching the bulb filter and power cord as explained below Installing or Replacing the Lamp Bulb 13 Fig 13 Before attaching the lamp bulb remove the parts that may drop such as the filter and specimen from the microscope frame and place the microscope on its back so that the bottom plate is exposed 1 Pull the lock knob 1 on the bottom to open lamp housing door figure 13 2 Hold the halogen bulb 2 without taking it out of the poly
20. with cleaning cloth slightly moistened with a mixture of petroleum 85 and isopropanol 15 Since solvents such as petroleum and isopropanol are highly flammable they must be handled carefully Be sure to keep these chemicals away from open flames or potential sources of electrical sparks for example electrical equipment that is being switched ON or OFF Also remember to always use these chemicals only in a well ventilated room Do not attempt to use organic solvents to clean the microscope components other than the glass components To clean non glass components use a lint free soft cloth slightly moistened with a diluted neutral detergent Donot disassemble any part of the microscope as this could result in malfunction or mitigated performance 7 T 4 When not using the microscope ensure that the frame is fully Seg cooled before storing the unit in a dry locker or covering with a Zo dust cover provided 1 5 To clean the condenser phase turret fully loosen the screw 1 T with the help of allen key and remove the condenser then wipe the front lens of the condenser with optical cleaning solution mixture suggested above and lens tissue The condenser can be re attached by replacing the condenser in its seat tightening securing screw and raising condenser Fig 3 bracket to desired position 6 Be sure to observe you
21. xact position desired AO 1mm vernier scale provides accurate location of specimen area High power illumination is delivered through our well crafted Universal Power Supply and operates with any 100V 240V AC input The LED configuration is operational with an in built rechargeable battery P N 9135000 902 which when plugged in draws power from a Universal Power Supply operating at 100V 240V Ac input This ensures continuous operation even under fluctuating voltages Our Halogen bulb 6V 20W has an average life span of up to 2 000 hours Our LED has an average life span of up to 60 000 hours The Lx 300 comes equipped with a removable N A 1 25 Abbe condenser with aspheric lens for brighter illumination levels and an iris diaphragm for better resolution and contrast control Lx 300 Z SAFETY INFORMATION 1 After the microscope has been used for observation of a specimen containing bacteria clean all parts coming in contact with the specimen to prevent infection Be sure to remove the specimen before moving this product In case the specimen is damaged by erroneous operation it is important to clean all surfaces that may have come in contact with the specimen 2 Toavoid potential electrical hazards when replacing either halogen bulb or LED turn the microscopes main switch to the OFF position and disconnect power cord from wall outlet in advance Whenever you replace your microscope bulb allow lamp socket and bulb to co
22. y loosening the screw SS 1 with allen key on right side of the condenser bracket as shown in figure 5 Place the phase annulus turret in position from where the condenser has been removed 90 a Secure it with screw 1 The phase turret can be adjusted at any Z desired phase annulus 10x 20x 40x amp 100x by turning the thumbwheel 2 to the desired position There is also an 0 setting on the thumbwheel for bright field application Hae Note Refer to the user manual provided with the Phase ig Contrast kit for centering and alignment Installing the Polarizing Kit 1 Remove the observation head from its position and install the analyzer by firmly placing the positioning pin of analyzer into the locating hole of dovetail adapter as shown in Fig 6 and Fig 7 Lx 300 2 Place the polarizer shown as 1 in fig 8 on the illumination assembly shown as 2 3 Fix the polarizer by tightening the screw provided Refer fig 9 Fig 9 11 e Lx 300 C INITIAL SETUP Objectives Objectives are factory set Objectives are par centered and par focalised during assembly All objectives have been secured for a tight fit to prevent them from coming loose during transit To remove an objective rotate it counterclock
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