TC-202A User`s Manual
Contents
1. Temperature Perfusion Rate Stability 37 C 0 ml min 4 0 1 C 37 C 2 ml min 4 0 1 C 15 C 0 ml min 4 0 1 C 15 C 2 ml min 4 0 1 C TEMPERATURE CHANGE TIME 10 90 25 C TO 37 C Plate Control Perfusion Rate Time 0 ml min 8 25 min 2 ml min 12 0 min Bath Control Perfusion Rate Time 0 ml min 8 5 min 2 ml min 7 0 min enuen SJ9s PILU erede yejpdougoj zrelodig vaoc L smeecicNy Mene 5 6 Harvard Apparatus TC 202A Bipolar Monopolar Temperature Controller User s Manual Introduction Model BSC BU Model PDMI 2 PSMI The Bipolar Monopolar Temperature Controller Model TC 202A is designed to be used with several microincubators supplied by Harvard Apparatus Inc These models include the Open Perfusion Micro 4ncubator Model PDMI 2 Patch Slice Micro Incubator Model PSMI Leiden Static Micro ncubator Model LU CB1 Leiden Closed Perfusion Micro Incubator Model LU CPC CEH and Brain Tissue Slice System Model BSC BU A microincubation system from Harvard Apparatus consists of the Temperature Controller TC 202A a micro incubator one of the above of choice a 35 mm plastic Petri dish or reusable cover slip dish LU CSD MSC TD or MSC PTD and an associat ed pumping system Such a system allow s complete environmental conditioning along with excellent micro mechanical access for electrodes or micropipettes all with opti mum optical access A proportional temperature controller is basi call2. MI cable to the chosen temperature controller s power control output In the cur rent version the PDMI 2 PSMI or CSMI is provided with a connector which matches theTC 202A output connector For other options the table below may be used In any case we suggest that you contact Harvard Apparatus Inc for instructions or more details PDMI 2 PSMI and CSMI Cable Wiring Main Cable Wire Size Original Temp Pin Out Final Peltier AWG 20 Red White Pinl amp 2 Red PowerGround AWG 20 Black Black Pin4 amp 5 Black Peltier Plates AWG 26 Green Green Shield Green Thermistor AWG 26 Blue Transparent Pin 6 White Thermistor AWG 26 Brown Red Pin3 Green Fin Jacks Wire Size Original Temporary Pin Out Final Ground AWG 26 N A N A N A Green Pin J ack 1mm Signal ground AWG 26 White White N A White Pin J ack 1mm salt bridge Appendix C Voltage and Fuse Settings 13 1 Make sure the power cord is NOT connected to the power entry module PEM in the back of the TC 202A 2 Using asmall flat screw driver open the door or cover of power entry module by prying at the grove on top 3 Pull voltage selector drum out and rotate to AC line voltage which will be used 100 200 220 240 Note Do not rotate drum while inside of power entry module center pins will break 4 To replace the fuses Pull out fuse drawer and snap off the two fuses Replace with fuses and reassemble the fuse drawer in to the power entry module Make sure arrows in fuse dra
3. Model TC 202A Bipolar Monopolar Temperature Controller User s Manual Model TC 202A Bipolar Monopolar Temp Controller 65 0045 Model TC 202AM Bipolar Monopolar Temp Controller 65 0046 Table of Contents Warranty and Repair Information 2 SPECIFICATIONS ariana akim dama 3 gg eg UE 4 5 Setting Up the TC 2024A 6 Front Panel Connectors and Controls 7 Operati OM PR et Haa do Ee eoe dnd dea xa E 8 Troubleshooting ies serian anda oni io eran dadas aie 9 A PDENG XOS H r irana aramam Er Rene sa ce ini 10 12 Appendix A I O Connectors 10 Appendix B PDMI 2 PSMI Wiring Color Codes10 Appendix C Voltage and Fuse Settings 11 Appendix D Rack Mounting the TC 202A 12 nue SSN PILO ame rol Jejdouorejodig v cOc L sme rede pene 3 Warranty and Repair Information B Un Harvard Apparatus TC 202A Bipolar Monopolar Tenperature Controller User s Manual Serial Numbers All inquires concerning our product should refer to the serial number of the unit Serial numbers are located on the rear of the chassis Calibrations All electrical apparatus is calibrated at rated voltage and frequency Warranty Harvard Apparatus warranties this instrument for a period of one year from date of purchase At its option Harvard Apparatus will repair or replace the unit if it is found
4. cilities and Parts Harvard Apparatus stocks replacement and repair parts When ordering please describe parts as completely as possible preferably using our part numbers If practi cal enclose a sample or drawing We offer a complete reconditioning service CAUTION This temperature controller is not registered with the FDA and is not for clinical use on human patients AN CAUTION Not for clinical use on human patients Specifications TC 202A Specifications Temperature Setting Range 0 to 50 C Temperature Regulation Temperature Display Chamber Temp Sensor Voltage Range Current Range Dimensions Weight Power AC Fuse AC Supply Voltage 0 2 C 0 1 C Thermilinear type 36 K nominal at 25 C 0 to 5 V 0 to 6 A DC sustained 48 3 cm x 8 9 cm x 33 7 cm 19 x 3 5 x 13 25 5 7 kg 12 5 Ib 150 W 2 fuses 1 Amp 120 V 2 fuses 0 5 Amps slow blow 220 V see use settings 115 VAC 60 Hz 220 VAC 50 Hz user selectable see volt settings The following refer to use with the PDMI 2 micro incubator and a Coming Petri dish as chamber mounted on a Nikon DiaP hot M inverted microscope A top cover is placed over the dish no gas flow is being used media depth in the dish is 4 mm TEMPERATURE STABILITY Plate Control Bath Temperature Perfusion Rate Stability 37 C 0 ml min 0 2 C 37 C 2 ml min 4 0 8 C 157 0 ml min 4 0 2 C 15 C 2 ml min 0 4 C Bath Control Bath
5. cubation system TC 202A micro incu bator and chamber has already been setup with appropriate interconnections see Specifications section on page 3 If being used with the PDMI 2 PSMI see the Setup and Operation section in the PDMI 2 PSMI or CSMI manuals for setup details First practice operation without perfusion or cells tissue in the attached micro incu bator PDMI 2 etc and chamber to allow you to better focus on the controller s oper ation Try plate control first set the DISPLAY to PLATE Hold the SET SENSE switch to SET while adjusting the SET dial to a temperature about 3 4o C above heating or below cooling the desired chamber temperature Release the SET SENSE switch and switch the DISPLAY to BATH You may wish to connect a strip chart recorder to OUT for a record of the approach of the chamber temperature to the set value Repeat the previous run with bath control Switch back and forth between PLATE and BATH display during this run the plate temperature may actually overshoot with the HEATING COOLING lamp temporarily reversing Finally try continuous perfusion With perfusion the initial temperature changes will occur faster but the final approach to the set point may take longer due to the extra loading of theTC 202A These practice runs should give a basis for deciding whether to use PLATE or BATH control as well as allow fine tuning of procedures If PLATE control is used allow for manual fine tuning of the c
6. e following assumes the TC 202A is being used with the Open Perfusion Micro Incubator PDM12 Display Reads 0 00 If this continues to happen despite the supply of heating cooling current indicated by the red green panel lamp then the BATH chamber thermistor isn t plugged in No Change in Temperature If this continues to happen despite the apparent supply of heating cooling current either the PDMI 2 or its cable has a discontinuity in electrical connection Measure the electrical resistance between pins 1 and 4 of the jack from the microincubator It should be 0 5 1 0 ohms Temperature Calibration is Off The sealed BATH thermistor BSC T3 is defective If its electrical resistance between the tip and ring of the jack at ambient 25 C temperature is much different than 36 Kohms then it should be replaced This is better tested with the thermistor in water since one failure mode is the development of a leak This will show up as a much lower resistance Negative Bath Temperature This also is due to a defective bath thermistor Its electrical resistance between tip and ring of the jack is probably infinite Negative Plate Temperature PDMI 2 PSMI and CSMI only This can happen normally during a change in temperature for a short time caused by overshoot of the plate temperature during BATH chamber control If it continues to read negative check the electrical resistance between pins 3 and 6 of the PDMI 2 cable at ambien
7. g Green Cooling Indicator This lamp indicates whether the temperature controller is heating or cooling the peltier chamber at the moment yo This multi pin panel jack accepts a matching plug from a chamber cable It includes inputs from the PDMI 2 s PSMI s or CSMI s internal temperature sensor thermistor cur rent output to drive the heat pumping and ground connections Pin out is given in APPEN DIX A Note LUCBI LUCPCEH BSC BU chambers are shipped with a cable that can be disconnected at the chamber and as well as at the TC202A panel These chambers do not have a plate thermistor and should always be used with a bath thermister as the control point Over Temp This lamp indicates when the TC 202A s output current has been temporarily disabled because the PDMI 2 s or PSMI s temperature driven plate has exceeded 50 C See Troubleshooting Section page 9 Set This allows setting of the desired target temperature Turn clockwise for higher tempera ture counterclockwise for lower temperature Push the set sense switch The target tem perature will be displayed on the LCD Control Point This switch selects the regulation control point either the chamber bath or the internal temperature driven plate PDMI 2 PSMI and CSMI only Plate control is safer if shallow media depths are being used with perfusion perhaps for rapid change of media where slight irregularities in flow rate may uncover the otherwise controlling
8. hamber temperature the exact temperature difference between plate and chamber depends on media perfusion rate recommended 9 2 ml min gas flow rate recommended Q 2 ml min media depth the presence or absence of atop cover and the mounting details on the microscope If being used for electrophysiological measurements PLATE control should be select ed for lowest noise the bath thermistor must be removed from the bath to avoid excessive transfer of noise signals to the recording electrode Prior measurement of the temperature difference between plate and bath for the chosen perfusion condi tions and media depth can be done to enable the chamber to be held at the desired temperature Monophasic Resistive Heater Micro Incubators The following assumes that a complete micro ncubation system TC 202A micro incu bator and chamber has already been setup with appropriate interconnections First practice operation without perfusion or cells tissue in the attached micro incu bator LU CB1 etc and chamber to allow you to better focus on the controller s oper ation Set the CONTROL POINT switch to the BATH setting Hold the SET SENSE switch to SET while adjusting the SET dial to a temperature about 379C Release the SET SENSE switch and switch the DISPLAY switch to BATH You may wish to connect a strip chart recorder to OUT for a record of the approach of the chamber tempera ture to the set value Troubleshooting ENNE DERE MN 11 Th
9. he bath thermistor become dislodged from the tissue media The temperature of this Peltier plate however will be 3 5 C difference from room temperature than the chamber itself See Specifications page 3 Model LU CSD Model LU CSD Special d Teflon Dish Teflon Dish Silicon Rubber Ring Me Multi Well Glass Coverslip g Silicon Rubber Ring Glass Coverslip Ee Stainless Steel Securing Ring Stainless Steel Securing Ring nue S PSN PILU erede seyjodouojp e odig VWZ0Z D_L smevedchy Mene Setting Up the TC 202A 8 LLL Harvard Apparatus TC 202A Bipolar Monopolar Temperature Controller Users Manual Use with the PDMI 2 PSMI or CSMI Peltier Type Chambers This section describes the electrical connections between the PDMI 2 PSMI or CSMI and theTC 202A To connect the 6 pin cable plug into the 1 0 Jack on the front panel of the TC 202A The A magnetic base thermistor holder for the bath thermistor is secured mechanically onto the chrome plated ferrous metal ring on the top black plas tic cover This thermistor is plugged into the Bath Sensor inlet of the TC 202A Before powering the unit on for the first time 1 Check that the TC 202A has been set to the proper AC line voltage 110 VAC 60 Hz 220 VAC 50 Hz on the rear panel 2 Insure that the switch on the rear panel of the temperature controller is fac tory preset for the type of chamber Peltier or resistive heater type that is shipped with t
10. he TC 202A CAUTION If the temperature controller and the chamber are purchased separately please set the rear panel chamber type selector for the correct chamber before use Use with Other Peltier Devices TheTC 202A can possibly be used with custom Peltier based temperature controlled chamber other than PDMI 2 and PSMI 2 Use with Resistive Heater Micro Incubators The TC 202A can be used with resistive heater micro incubators such as the Leiden Static Micro Incubator Model LU CB1 Leiden Closed Perfusion Micro Incubator Model LU CPC CEH and Brain Tissue Slice System Model BSC BU A switch on the rear panel of the temperature controller is factory preset for the type of chamber Peltier or resistive heater type that is shipped with the TC 202A For details of each micro incubator refer to the specific micro incubator s user manuals chased separately please set the rear panel chamber type selec CAUTION If the temperature controller and the chamber are pur tor for the correct chamber before use Front Panel Connectors and Controls A 2 Medical Systems E Ae Research Products TEMPERATURE CONTROLLER conTROL MONITOR DISPLAY BATH MAINS uo ser oir SET PLATE POINT o 37 5 RED HEATING o iL e T au c iens GREEN COOLING OVER TEMP 100 mV iC i CENTIGRADE SENSE BATH Power This push button on off switch for main power glows red when on Push again to turn off Red Heatin
11. t temperature If infinite or much greater than 100 Kohms then the PDMI 2 should be returned for replacement of the internal thermistor Fue S PSN PILU erede Jejpdougo zrelodig VWZ0Z D_L smevedchy Mene Over Temp If the plate temperature goes above 75 C then the TC 202A stops delivering current and this panel light goes on until the temperature returns to safe values This likely due to feedforward failure of the Peltier devices inside the microincubator No dam age has been done to the micro incubator or the TC 202A See TROUBLESHOOTING in the specific microincubator manual for suggested changes in operating procedure to avoid this in the future Appendix A I O Connector DO 700 Harvard Apparatus TC 202A Bipolar Monopolar Temperature Controller User s Manual Peltier chamber power output when heating if cooling Resistive Heater chamber P ower Output Thermistor input from temperature driven plate of PDMI 2 or PSMI Connected together in the jack Chamber Power Ground Connected together in the jack Chamber Power Ground Thermistor input from temperature driven plate of PDMI 2 or PSMI ao 0 BEN Appendix B Wiring PDMI 2 PSMI and CSMI Color Codes From the first release to the market of the PDMI 2 PSMI and CSMI Micro Incubators to the current design there have been various design changes A wiring color code of the different units released is provided below to aid the wiring of the PDMI 2 PSMI or CS
12. thermistor Note When using the TC202A in monopolar the plate control will be disabled or will not have any effect on the BSC BU LU CBI or LU CPCCEH microinaubators and temperature can be controlled only when the bath thermis tor isin the chamber Out BNC Panel Connector This electrical output monitors either the bath or plate temperature depending on the position of the DISPLAY switch This is useful for monitoring temperature change proto cols with a computer or strip chart The scaling factor is 100 mV o C Centigrade This 3 5 digit digital display gives the temperature selected by the two DISPLAY switches Display This switch selects temperature source for display The switch on left is spring loaded so it Sits at SENSE It reads the current temperature sensed by either the chamber BATH ther mistor BSC T3 or the plate thermistor inside the PDMI 2 PSMI or CSMI In SET position the digital display reflects set point target temperature as commanded by SET control The Switch on the right selects temperature sense location being displayed BATH or PLATE Bath Sensor This 1 4 inch phone jack accepts the BATH chamber thermistor BSC T3 Use BSC T3A short version with LUCPC CEH chamber nue S PSN PILU erede Jejpdougoj relodig vaoc 9 L smevedchy Mene Operation DO 7 a aaa Harvard Apparatus TC 202A Bipolar Monopolar Temperature Controller User s Manual PDMI 2 PSMI and CSMI Thefollowing assumes that a complete micro in
13. to be defective as to workmanship or material This warranty does not extend to damage resulting from misuse neglect or abuse nor mal wear and tear or accident This warranty extends only to the original customer purchaser IN NO EVENT SHALL HARVARD APPARATUS BE LIABLE FOR INCIDENTAL OR CONSEQUENTIAL DAMAGES Some states do not allow exclusion or limitation of incidental or consequential damages so the above limitation or exclusion may not apply to you THERE ARE NO IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR USE OR OF ANY OTHER NATURE Some states do not allow this limitation on an implied warranty so the above limitation may not apply to you If a defect arises within the one year warranty period promptly contact Harvard Apparatus Inc S October Hill Road Building 7 Holliston Massacbusetts 01746 1371 using our toll free number 1 800 272 2775 Goods will not be accepted for return unless an RMA returned materials authorization number has been issued by our customer service department The customer is responsible for shipping charges Please allow a reasonable period of time for completion of repairs replace ment and return If the unit is replaced the replacement unit is covered only for the remainder of the original warranty period dating from the purchase of the original device This warranty gives you specific rights and you may also have other rights which vary from state to state Repair Fa
14. wer if any are pointing to the same direction Note Make sure you have proper fuse rating for AC line voltage selected Example 100 120 VAC 1 amp slow blow 2 220 240 VAC 5 amp slow blow 2 5 Close by snapping power entry module door making sure AC line voltage select ed can be seen through the window of door for vit m C Bay r pan nue SJ9s PILU amed Jejdougoj relodig veaoc L smeveddhy Mene bos ileriki DEBER m Tip jra i E77 y Fan i P o Appendix D Rack Mounting the TC 202A A Harvard Apparatus TC 202A Bipolar Monopolar Temperature Controller User s Manual Place unit on a hard flat Remove three screws on Position the mounting Insert the three screws and Repeat step four on the Insert the unit into the 19 CAUTION Turn power OFF before attaching mounting bracket surface with front facing towards you either side of the unit bracket over the screw holes as shown below fully tighten with a suitable screw driver other side of the unit inch rack and screw with suitable screws not provided
15. y a source of electric current whose magni tude is determined by comparing a desired tar get temperature with its current value TheTC 202A s output is proportional to the difference in temperature between the desired and the current value This type of system avoids the electrical noise of suddenly turning current on and off unlike less expensive controllers When using theTC 202A in the Biphasic mode the current will spontaneously reverse direc tion when the current temperature is too high in short it can also cool when used with Peltier elements PDMI 2 PSMI Model LU CPC CEH Introduction Model LU CB1 M Model HAI PTD amp HAI TD TheTC 202A features two complementary temperature controlling options 1 The chamber temperature can be controlled using the supplied thermistor BSC T3 This option allows the desired chamber temperature to be dialed directly on the SET control This option has the advantage of better stability of temperature over time allowing faster change of temperature See Specifications page 3 2 The temperature can be controlled with the internal thermistor PDMI 2 and PSMI only The connection to this internal thermistor is made through the integral multi conductor cable This internal thermistor measured the temperature driven plate of the devices the incoming perfusate is heated or cooled by this plate This mode of operation prevents the chamber overheat ing or over cooling should t
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