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Quality Assurance 2 April 2005

1. Physical Habitat Inventory Site ID Date Transect Substrate Cross Stream Habitat Section Information Station Thalweg Depth cm Type Flag Depth 3 ag cm Size Class Flag O 2 a 0 Left Bank gt 2 2 1 One quarter ome g 2 20 p o o Half way BAUS ZB 3 923532298 Three quarters 3320728 4 Right Bank ee 58 5 Total wetted width o o m y 2 P gt gt 6 Substrate Size Class Codes 207052 8 RS Bedrock smooth Larger than a car 9 RR Bedrock rough Larger than a car 10 RC Concrete Asphalt 11 XB Large boulder meterstick to car 12 SB Small boulder basketball to meterstick 13 CB Cobble tennisball to basketball 14 GC Coarse gravel marble to tennisball GF Fine gravel ladybug to marble Stream Habitat Types ER riffle glide cascade rapid or waterfall SA Sand gritty to ladybug DE pool FN Silt clay muck not gritty HP Hardpan firm consolidated fine substrate Substrate Only Stn 5 or 7 LFT LCTR CTR WD Wood OT Other flag and describe Tally of Large Wood in Stream at amp between Transects Shade Covering Channel 30 upstr 30 dwnstr pieces 1 2 dia large end lt 40 40 70 gt 70 pieces gt 2 dia large end Riparian Zone 30 X 30 plot from edge of stream Invasive Plant Species circle all present Left Bank Right Bank blackberry Eng ivy reed canarygrass none Trees gt 15 tall D CMN D C MN conifers gt 1 dbh Comments Understory None Spar Mod De
2. Table 1 shows the distribution of land ownership and Table 2shows the proportion of land use and acreage in each sub watershed The watershed s population in 1990 was estimated to be 92 000 The greatest concentration of people is located in the urban portions of the watershed chiefly Eugene Veneta and Monroe Farming ranching logging and rural residential development are other significant land uses Table 1 Long Tom Watershed Ownership Ownership Acres Percentage Private 242 131 92 BLM 295 lt 1 O amp C Lands 20 355 8 State Lands 66 lt l Land use acreage was determined from state wide zoning maps This calculation was based on digitized census block information from the 1990 U S Census LTWC Quality Assurance Project Plan July 2004 2006 5 Table 2 Sub basin Land Use Sub basin Agri Forestry Urban Rural Parks Rural Other Total culture Resident amp Rec Indust Acres Upper Long 8 80 lt 1 10 2 lt 1 0 35 605 Tom R Elk Cr 9 88 0 1 0 1 0 27 709 Coyote Cr 31 64 0 4 2 0 0 45 185 Spencer Cr 22 49 1 27 lt 1 0 0 21 320 Upper 6 6 80 1 lt 1 0 0 19 710 Amazon Cr Lower 62 0 21 6 lt 1 0 11 19 292 Amazon Cr Fern Ridge 25 20 5 20 5 0 25 32 209 Bear Cr 33 57 0 10 lt 1 0 0 17 701 Ferguson 40 59 0 lt 1 0 0 0 16 357 Cr Lower Long 81 1 1 8 2 0 lt 1 27 78
3. AUDIT VALUES Water Temperature Air Audit Thermometer ID Temperature Audit Logger Audit Logger_ Comments STATUS PO O O IO RS a a Date Downloaded Time Time Shift Grade Computer Post Deployment Temperature Check Thermometer ID Date Thermometer ID Date Low Temp TEMP TEMP High Temp TEMP TEMP TIME MASTER UNIT Difference STATUS TIME MASTER UNIT Difference STATUS COMMENTS LTWC Quality Assurance Project Plan July 2004 2006 32 Appendix G Macroinvertebrate Monitoring Survey Design Description of Sample Design Target population All streams rivers in the Long Tom watershed Watershed boundaries as defined in Long Tom boundary coverage Sample Frame RF3 alpha version with Strahler order Restricted to Long Tom Watershed boundary and excluding streams in Willamette and Fern Ridge Reservoir subbasins Subbasins are identified in the NAME attribute Survey Design A Generalized Random Tessellation Stratified GRTS survey design for a linear stream resource was used The GRTS design includes reverse hierarchical ordering of the selected sites Multi density categories 10 subbasins within the Long Tom Stratification None Panels 6 panels Annual panel may be visited once every year Rotating panels Yearl to Year5 are to be visited once every 5 years Expected sample size 10 in each subbasin over the five years for a total of 100 sites Oversample 200 200 for a total of 300 sites Site Use Th
4. x Albers x coordinate y Albers y coordinate LonDD Longitude decimal degrees NAD27 LatDD Latitude decimal degrees NAD27 mdcaty Multi density categories used for unequal probability selection weight Weight in meters inverse of inclusion probability to be used in statistical analyses stratum Strata used in the survey design panel Identifies base sample by panel name and Oversample by OverSamp auxiliary variables Remaining columns are from the sample frame provided Projection Information Albers projection used Datum NAD 27 Spheroid Clarke1866 Units meters Center longitude decimal degrees 96 Origin latitude decimal degrees 23 LTWC Quality Assurance Project Plan July 2004 2006 34 Standard parallel 1 decimal degrees 29 5 Standard parallel 2 decimal degrees 45 5 Evaluation Process The survey design weights that are given in the design file assume that the survey design is implemented as designed That is only the sites that are in the base sample not in the over sample are used and all of the base sites are used This may not occur due to 1 sites not being a member of the target population 2 landowners deny access to a site 3 a site is physically inaccessible safety reasons or 4 site not sampled for other reasons Typically users prefer to replace sites that can not be sampled with other sites to achieve the sample size planned The site replacement process is described abov
5. 1 2 3 SATA Place the meter on a flat stable surface or leave in blue box Turn meter on 1 O button Make sure the machine is in auto range auto rng is indicated in lower left corner If it s not then push the range button until it shows this Do an accuracy check using the bottles with numbered labels on top If you have more than one site you only need to do the accuracy check at your first site Here s how to do an accuracy check a Place a drop of oil on bottle of 1 standard around 5 and wipe off with the black cloth b Insert it into the slot in the meter so that the white diamond on the bottle aligns with the mark at the front of the bottle slot on the meter c Close lid and press the read button Record results in the logbook d Follow the same procedure for the other two numbered vials one is around 50 the other around 500 Rinse sample bottle with stream sample water 2 3 times If sample has been sitting then gently shake it before filling sample vial Pour stream sample into the rinsed sample vial Wipe off vial with a soft absorbent cloth Place vial in meter being sure to align mark on vial with mark on meter Close lid push the read button and record reading on data sheet When finished turn machine off clean the sample vial with distilled water and return it to the box Conductivity amp Water Temperature If you are the first person using the meter today you need to do an
6. are rinsed carefully inspected for clinging macroinvertebrates and removed As much fine sediment as possible should be washed away Leaf packs from pool samples may require considerable rinsing and removal of debris before preserving the composite sample When finished sampling all 8 ft sieve sample through 8 brass sieve Place all insects or material that may have insects on it into Nalgene bottles Do no fill bottles up more than 25 by volume with organic matter Place a label Rite in the Rain paper written in pencil containing site and habitat unit information inside the container Label the outside container with a pencil written on a label then tape the label to the outside of the jar Do not use markers as most inks are soluble in alcohol Then pour enough ethanol to cover sample until you get back to the car When you get back to the car fill bottles up with ethanol to completely preserve them 10 Fill out Targeted Riffle Benthos section in the Sample Collection Form In the comments section describe the habitat types you sampled from e g all riffles 1 riffle 3 pools etc LTWC Quality Assurance Project Plan July 2004 2006 40 Appendix J Physical Habitat Data Sheet LTWC Quality Assurance Project Plan July 2004 2006 41
7. gt Record stream habitat type riffle cascade run glide or pool gt Tally large wood The last steps are to fill out the human disturbance inventory form for the reach and do a written assessment of stream condition can do this one driving to next site or on your way back LTWC Quality Assurance Project Plan July 2004 2006 37 Appendix I Oregon DEQ Benthic Macroinvertebrate Protocol for Wadeable Rivers and Streams LTWC Quality Assurance Project Plan July 2004 2006 38 Benthic Macroinvertebrate Protocol for Wadeable Rivers and Streams Revised July 18 2003 Background Evaluating the biological community of a stream through an assessment of the macroinvertebrates provides a sensitive and cost effective means of determining stream condition The goal of the protocol described in this section is to collect an unbiased representative sample of benthic macroinvertebrates in wadeable streams and rivers At each stream reach samples are collected by compositing eight D Frame Net kick samples from a selected habitat unit e g riffles fastest flowing water Samples are preserved in the field with ethanol Field Collection Methods Equipment and Supplies e 500 um mesh D Frame kick net 5 gallon bucket Scrub brush forceps Long sleeved rubber gloves Nalgene containers Sample labels Waders with slip resistant soles Targeted habitat sampling 1 Beginning at the downstream end of the reach select the first riffle or pool habitat unit
8. push button in and down on the titrator until it contacts the cartridge Take cap off cartridge rinse a delivery tube with distilled water and insert into cartridge Turn the black dial on top of the titrator clockwise and allow a little of the sodium thiosulfide to come out Just enough to remove air bubbles from the line Zero titrator by turning dial next to counter Slowly add sodium thiosulfide to sample by submerging the delivery tube into the sample Swirl flask while you do this to mix When the sample turns pale yellow add 1 ml of starch 1 dropperful to the sample swirl to mix the sample will turn blue black If the sample is pale yellow to begin with you can add the starch before you add any sodium thiosulfide Continue adding sodium thiosulfide with digital titrator swirling as you add it until sample is clear Go very slowly when the sample becomes pale so you don t overshoot the mark Enter the number on the titrator onto the data sheet Put cap back on sodium thiosulfide cartridge pull back plunger and remove cartridge from titrator Rinse all test containers with distilled water and replace in case LTWC Quality Assurance Project Plan July 2004 2006 26 Turbidity meter Note if you are storing the equipment overnight be sure to keep it indoors so it does not get cold A cold turbidity meter gives erroneous readings If it has accidentally gotten cold let the machine warm up in your car or house before using it
9. 11 12 13 Bob Hager Len Gillette February 8 9 10 Rolf Anderson Erik Osborn amp Jack Detweiler Brian Greene March 8 9 10 Bob Hager Cathy Glaudin amp Paul Atkinson Carl DiPaolo April 5 6 7 Len Gillette Paul Reed Dave Turner May 10 11 12 Rolf Anderson Erik Osborn amp Jack Detweiler Brian Greene June 7 8 9 Bob Hager Cathy Glaudin amp Paul Atkinson Carl DiPaolo July 5 6 7 Len Gillette Paul Reed Dave Turner August 9 10 11 Rolf Anderson Erik Osborn amp Jack Detweiler Brian Greene September 6 7 8 Bob Hager Cathy Glaudin amp Paul Atkinson Carl DiPaolo October 11 12 13 Len Gillette Paul Reed Dave Turner November 8 9 10 Rolf Anderson Erik Osborn amp Jack Detweiler Brian Greene December 6 7 8 2006 January 10 11 12 Bob Hager Cathy Glaudin amp Paul Atkinson Carl DiPaolo Len Gillette Paul Reed Dave Turner February 7 8 9 Rolf Anderson Erik Osborn amp Jack Detweiler Brian Greene March 7 8 9 Bob Hager Cathy Glaudin amp Paul Atkinson Carl DiPaolo April 10 11 12 Len Gillette Paul Reed Dave Turner May 8 9 10 Rolf Anderson Erik Osborn amp Jack Detweiler Brian Greene June 6 7 8 Bob Hager Cathy Glaudin amp Paul Atkinson Carl DiPaolo LTWC Quality Assurance Project Plan July 2004 2006 Appendix E Field Data Sheet Site ID Sampler s Date Time Ai
10. 14 15 16 17 18 19 20 21 Dissolved Oxygen Winkler titration Thoroughly rinse glass DO bottle with stream sample water Then fill the DO bottle by either placing it in the collection bucket or submerging into the stream by hand when stream depth is too shallow for bucket Fill the bottle until it overflows and make sure that no air bubbles are trapped in the bottle Insert stopper Don t let the bottle sit around Do the next steps right away or the DO may change It is recommended that you put gloves on at this point Also avoid getting reagents on your skin or clothing Add powder 1 manganous sulfate amp then powder 2 alkaline azide Shake bottle vigorously for 30 seconds Wait until the particulates settle 1 2 way down bottle Again shake bottle vigorously for 30 seconds Make sure there are no large undissolved chunks of reagent left Wait until the particulates settle Ya way down bottle Add powder 3 sulfamic acid Be sure to avoid getting this on your clothes Shake until sample is clear amber Rinse both the graduated cylinder and flask first with distilled water and then with a little of the amber sample before measuring and pouring into flask Measure out 200 ml of sample in graduated cylinder and pour it into the 250 or 500 ml flask whichever is in your kit Load black titrator with sodium thiosulfide cartridge by sliding 1t all the way into the slot and twisting 90 Lower the plunger
11. 30 Meter Instream or none immediately sampling bucket Turbidity HACH 2100P Meter Screw top none immediately bottle Total Phosphorus EPA 365 3 125 mL plastic Acidified to 28 days bottle pH lt 2 stored lt 4 C Nitrate Nitrite N EPA 353 3 125 mL plastic Acidified to 28 days bottle pH lt 2 stored lt 4 C E coli Colilert QT IDEXX 120 mL plastic none 24 hours laboratories bottle Macroinvertebrate and Physical Stream Habitat Monitoring The macroinvertebrate program also relies on volunteers to collect macroinvertebrate samples and collect physical habitat data at each site Trainings will be held each summer for new and returning volunteers In addition the monitoring coordinator or program assistant will review each team s technique at their first site for the season The monitoring coordinator will identify and contact the landowner for each randomly selected 66 99 x site If permission is granted volunteers are given driving directions and any special instructions to reach the site a topographic map of the area an aerial photo GPS coordinates of the x site and landowner contact information Volunteers will use the maps and aerial photos to reach the general location of the site and locate the x site using a handheld GPS In the event that landowner permission is not granted or the site is unsampleable because it is dry unsafe to access etc a new site will be selected from the Ov
12. Quality Thieman 2003 Water Quality in the Long Tom River Watershed 1999 2003 Report prepared for the Long Tom Watershed Council LTWC Quality Assurance Project Plan July 2004 2006 21 Appendix A 2002 303 d List of Water Quality Limited Streams in Long Tom Watershed River or Stream Amazon Creek RM 0 22 6 Amazon Creek RM 0 22 6 Amazon Creek RM 0 22 6 A 3 Channel A 3 Channel A 3 Channel A 3 Channel A 3 Channel A 3 Channel Amazon Diversion Channel RM 0 1 8 Amazon Diversion Channel RM 0 1 8 Ferguson Creek RM 0 10 Fern Ridge Reservoir Fern Ridge Reservoir Coyote Creek RM 0 26 2 Coyote Creek RM 0 26 2 Willow Creek RM 0 2 8 Lower Long Tom Lower Long Tom LTWC Quality Assurance Project Plan Characteristic Arsenic Lead E coli dichloroethylenes tetrachloroethylene Arsenic Lead Mercury E coli Fecal coliform Dissolved oxygen Temperature Turbidity Bacteria Dissolved oxygen Bacteria Arsenic Temperature Bacteria July 2004 2006 Data Source sampling period City of Eugene sampling period not specified City of Eugene sampling period not specified City of Eugene sampling period not specified DEQ sampling period not specified DEQ sampling period not specified DEQ sampling period not specified City of Eugene sampling period not specified City of Eugene sampling period not specified City of E
13. Watershed Council Phil Larsen 541 754 larsen phil epamail epa Technical Advisory EPA 4362 gov Member Laboratory QA Officer Kevin Hunt 689 3177 delta clipper net Delta Environmental Water Quality Monitoring Bob Hager 344 2296 Team Members Len Gillette 998 7517 John Dillard 847 5761 jtdillard monrotel com Brian Green 847 5761 Cathy Glaudin 344 9961 cglaudin pond net Paul Atkinson 345 2186 Rolf Anderson 688 4949 Paul Reed 342 7064 preedOefn org Jack Detweiler 344 9000 jdetweiler brwncald 686 9915 Dave Turner 998 6889 Carl DiPaolo 484 6351 Distribution List LTWC Water Quality Cindy Thieman 683 2983 cindythiemanO yahoo Monitoring com Coordinator DEQ Volunteer Water Steve Hanson Laboratory 503 229 HANSON Steve deq st Quality Monitoring Division 5449 ate or us Coordinator DEQ Project Manager Rachel Burr 686 7838 burr rachel O deq state ext 264 or us Watershed Council Tom Mendes 682 8624 Thomas M Mendes ci e Technical Advisory City of Eugene ugene or us Member Watershed Council Phil Larsen 541 754 larsen phil epamail epa Technical Advisory EPA 4362 gov Member Contract Review Chair Jack Detweiler 344 9000 jdetweiler brwncald and Monitoring Team 686 9915 Member LTWC Quality Assurance Project Plan July 2004 2006 Introduction The Council received funding from the Oregon Watershed Enhancement Board OWEB and the Oregon Department of Environmental Quality EPA 319 Funds to
14. accuracy check If one has already been completed that day skip to 2 Accuracy check instructions a After thoroughly rinsing probe and shaking off excess water place it in the container labeled conductivity standard b Turn machine on and make sure it is in temperature compensating mode This is indicated when the Celsius symbol C on the bottom right is flashing If it isn t push the mode button until you see this feature Conductivity units are microsiemens per centimeter uS cm and temperature is in C c Stir probe slowly in the standard solution without touching the sides or bottom of the container Make sure the hole on the side of the probe is submerged and doesn t have an air bubble trapped in it d When the reading has stabilized enter the conductivity and temperature reading in the conductivity logbook e Put cover back on conductivity standard do not discard f Skip to 4 Turn machine on Make sure it is in temperature compensating mode This is indicated when the Celsius symbol C on the bottom right is flashing If it isn t push the mode button until you see this feature Conductivity units are microsiemens per centimeter uS cm and temperature is in C Thoroughly rinse probe with distilled water and shake off excess water Measure water temperature and conductivity by placing probe into the blue bucket or the stream being sure not to let the probe touch the sides of the container or bottom of stre
15. and shake as directed Continue the process doing each progressive step on one bottle then the other Also fill two vials for the turbidity duplicate sample at the same time and measure one right after the other For conductivity and temperature measure once from the bucket or stream and record results then measure again and record results Are your duplicate results 0 5 mg L for dissolved oxygen 2 for conductivity 5 for turbidity This is what each of these parameters needs to be to get an A grade If your duplicate result for any of the parameters does not meet the above standard try again LTWC Quality Assurance Project Plan July 2004 2006 24 Equipment List LTWC Quality Assurance Project Plan Dissolved oxygen kit contains powder pillows glass DO bottle with stopper graduated cylinder for measuring out sample scissors titrator sodium thiosulfide cartridge delivery tubes starch and 500 ml Erlenmeyer flask Rubber gloves Liquid waste container Distilled water Safety vest July 2004 2006 Turbidimeter contains meter sample vials standards oil black cloth drying cloth logbook Conductivity meter and logbook Conductivity standard Hand sanitizer Data sheets in plastic folder in box 2 packs of AA batteries amp 9 V battery Measuring tape Blue sampling bucket Extra D O bottle for duplicates 1 extra liter of distilled water thermometer 25 yY OS RA 12 13
16. it hits the point on the bridge i e white paint mark and then pull the bucket up Use the measuring tape to measure from the end of the bucket to the point on the rope that you have marked with your fingers Record on data sheet Several times during the year I will go out to these sites and measure stream flow and also the height from stream surface to bridge This will allow me to develop a graph like the one below By developing a graph like this we can later calculate what the stream flow was when monitoring occurred Stream flow based on distance from bridge to water surface 16 oO g 14 5 12 n 5 ae 10 ss 8 ob e Stream D flow 4 2 0 19 18 18 5 14 11 Stream flow cubic feet second LTWC Quality Assurance Project Plan July 2004 2006 28 Appendix C DEQ Data Quality Matrix Available at http www deq state or us lab qa DEQ04 LAB 0003 GD pdf LTWC Quality Assurance Project Plan July 2004 2006 29 Appendix D Sampling Schedule and Duplicates Sampling Dates 2004 July 6 7 8 People Doing Duplicates Erik Osborn amp Jack Detweiler Dave Turner August 10 11 12 Rolf Anderson Brian Greene John Dillard September 7 8 9 Bob Hager Len Gillette Cathy Glaudin amp Paul Atkinson October 12 13 14 Erik Osborn amp Jack Detweiler Carl DiPaolo Paul Reed November 7 8 9 Rolf Anderson Brian Greene John Dillard Dave Turner December 7 8 9 2005 January
17. rifles at all sites pools only if no riffles present Collect one kick sample from each riffle or poo unless fewer than eight are present within reach In that case evenly spread the eight samples across the number of riffles or pools within the reach EXCLUDE margin habitats area within 5 of channel margins Vissualize a 3x3 grid over each riffle or habitat unit to be sampled see figure 1 For the first habitat unit select the lower left square for the second habitat unit select the lower center the third the lower right for the fourth select the middle left for the fifth select the middle center for the sixth select the middle right for the seventh select the upper left for the eigth select the upper center Collect the kick sample in the center of each grid square E 8 9 4 5 6 1 2 3 Figure 1 Visualize a grid overlay to select kick sites at each habitat unit riffle or pool N After locatine the random sample location place the net into the stream with the flat part of the hoop resting on the bottom and perpendicular Lo the stream flow As much as possible make sue iv remove any substrate that prevents the flat part of the kicknet from sitting flush with the bottom It may also be useful to remove large substrate particles downstream of the flat portion of the loop that may affect the flow entering the net Collect the macroinvertebrate sample by disturbing a 30 by 30 centimeter area 1ft x 1 ft LTWC Q
18. technical support from the monitoring coordinator throughout the project period Field monitoring will be conducted using the standard protocols described in the OWEB Water Quality Monitoring Guidebook for stream temperature turbidity conductivity and dissolved oxygen Please see Appendix B for sampling order duplicate sampling instructions and equipment instruction sheets that are used by the monitoring team in the field Table 5 lists the equipment specifications and holding times for each parameter LTWC Quality Assurance Project Plan July 2004 2006 13 Volunteers will record a correlate for streamflow by measuring the distance from a fixed point above the stream e g usually fixed point on bridge to the surface of the water Stream flow values will then be estimated by correlating actual stream flow data with these measurements for each site The monitoring coordinator will collect the stream flow data using standard USGS flow measuring devices At least 4 measurements will be taken at each site at different stream levels to determine the relationship between stream flow and stream height Field measurements will be recorded immediately after the sample is collected both on the data sheet and in each volunteer s personal logbook The only exception is for titration of the dissolved oxygen sample Once all three powder reagents have been added to the sample it can be stored in the refrigerator for up to 8 hours before titrating However
19. 4 Tom R Watershed 31 46 8 9 1 1 4 262 872 Total Summary of Results from Phase 1 Monitoring Results from our water quality monitoring program from September 1999 through June 2003 indicate a number of water quality issues in the basin Thieman 2003 The most striking data are the high E coli levels in several sub watersheds As shown in earlier studies Amazon and Coyote Creek have chronic E coli problems City of Eugene 1999 Army Corps of Engineers 1999 Lane Council of Governments 1983 In addition very high levels have been found in Bear Creek Ferguson Creek and at the outlet of Fern Ridge Reservoir Data for dissolved oxygen and water temperature indicate problems at all downstream sites during the summer months Both nitrate and phosphorus are a concern in the upper and lower Amazon sub watersheds and the lower Long Tom River The upper Amazon sub watershed drains the City of Eugene and the lower sub watershed and the lower Long Tom River drain high density irrigated cropland We suspect that phosphorus is the primary limiting nutrient in Long Tom Watershed streams and lakes and the high levels we are seeing may be a significant factor influencing algal growth biological oxygen demand and in turn dissolved oxygen levels However it is possible that nitrogen may become a limiting nutrient in the Amazon sub watersheds during the summer when algal photosynthesis is at a high point Conductiv
20. EQ along with the temperature data see Appendix F Nutrient and Bacteria Sampling The monitoring coordinator and program assistant will collect the nutrient and bacteria samples Surface water samples for E coli and nutrient analysis will be collected within a 24 hour period and kept on ice during transport As described in the monthly field monitoring methods samples will be collected by wading or from a bridge using a bucket They will be taken to Delta Environmental Laboratories within 24 hours of collection and each sample will be marked with the sample ID number and time and date of collection A chain of custody record will be submitted to Delta Environmental upon delivery of samples see Appendix K for example Delta Environmental will send a copy of the chain of custody and results to the Watershed Council approximately one month after the sampling date The analytical methods and specifications for nitrate total phosphorus and E coli are listed in Table 5 below LTWC Quality Assurance Project Plan July 2004 2006 14 Table 5 Specifications for Monitoring Equipment and Analytical Methods Parameter Equipment Method Container Preservation Holding Time Water NIST Traceable Instream or none immediately Temperature Thermometer bucket single Water Vemco datalogger Instream none N A Temperature continuous Dissolved Oxygen HACH OX DT Kit 300 ml BOD Winkler 8 hr btl Titration Conductivity YSI Model
21. NA NA NA NA NA NA Willamette Site Selection Summary Number of sites by subbasin and Panel Bear Creek Coyote Creek Elk Creek Ferguson Creek Fern Ridge Lower Amazon ower Long Tom S Spencer Creek Upper Amazon Upper Long Tom 2 N O e a N Annual OverSamp Yearl Year2 Year3 Year4 Year5 18 2 2 3 2 2 16 1 4 1 3 1 14 2 3 2 3 4 15 2 3 ds 3 3 15 4 1 1 3 2 20 1 2 1 2 2 14 4 2 5 2 2 11 3 2 4 1 5 13 3 3 3 2 3 14 3 3 4 4 1 Description of Sample Design Output To achieve an expected sample size of sites in the target population an appropriate sample size was selected for the study area A Base set of sites and an Oversample of sites are included in the output The oversample sites should be added as needed in numerical SiteID order Oversample sites are identified in the panel data column as Oversamp Note that sites may be used in order beginning at the first SitelD number and continuing until desired sample size is reached If do not want to use the annual panel then use panels Yearl to YearS Can combine two panels together if want to visit double the number of sites within a year A map of the stream network and the selected sites is given in the accompanying pdf file The tab delimited ASCII file BullTroutSites tab has the following variable definitions Variable Name Description SiteID Unique site identification character arcid Internal identification number
22. Quality Assurance Project Plan Phase 2 Water Quality Monitoring Program Long Tom Watershed Council July 2004 June 2006 Monitoring Coordinator Project QA Officer Date Cindy Thieman Contract Review Chair Date Long Tom Watershed Jack Detweiler Council Technical Advisory Committee Chair Date Phil Larsen EPA DEQ Volunteer Monitoring Date Coordinator Steve Hansen DEQ Quality Assurance Date Officer Chris Redman LTWC Quality Assurance Project Plan July 2004 2006 Table of Contents PROJECT NI AA RON 3 DISTRIBUTION ETS Tous arrarnta E sessseeeseevebusssovesesdeeves nase R S EEN E TON RE 3 INTRODUCE AAA ON 4 WATERSHED OVERVIEW isiccncssccccvssessescscutessvssscdesvetsseepustestossaustvesessesvesesessxebcsdestelessuteesessesteiassedeosebbeitesesbese sseseassewtesesseseees 4 SUMMARY OF RESULTS FROM PHASE 1 MONITORING uu scccsssssccssssccsssscccssssccccsscccccssssccssseccsssscscsssscsccssseees 6 OBJECTIVE Suicidio sve cvencsucedessuccssovusiesscswevesvabecseatussedoosesssbuavesestebesseesessddusveocosduccscswbse st onsedeesessesdsveaseesedeavesestext 7 ICON IR INES AAA sassscessactienssosess cnssedessesscssssonseddevenssesseusdesdwsesssbacesos stdeascaueses 7 STUDY DESIGN A A RT 7 SA RN 13 CONTINUOUS TEMPERATURE MONITORING neissen nn nn nono nn nn nn nn nn nro nn non nnnnnnnnnnnnnnnnonononononononononenenenonenes 14 NUTRIENT AND BACTERIA SAMPLING ccccccccccccccccccesceeccsesesesesese
23. a Temp NO3 agriculture rural residential ml z g Upper Long Tom Alderwood UL2 D O Cond Turb Forestry livestock rural 51 44 1544 123 4239 a State Park Temp NO3 E coli residential E LTWC Quality Assurance Project Plan July 2004 2006 11 Macroinvertebrate and Physical Stream Habitat Monitoring A significant addition to our monitoring program is the collection of macroinvertebrates and stream habitat data at 100 randomly selected sites Each of the sub watersheds will have approximately 10 randomly selected sites located within them This sampling strategy will enable us to assess the biological conditions of each sub watershed as well as differences between forestland agricultural rural residential zones and urban areas These data will also provide us with an important baseline of information which will allow us to establish long term biological monitoring The survey design and generation of randomly selected sites was provided by Tony Olsen in the Environmental Statistics Section at the Corvallis Branch of the Environmental Protection Agency Please see Appendix G for a complete description of the sampling design Macroinvertebrates will be collected during the summers of 2004 and 2005 between June and September Upon recommendation of Rick Hafele Oregon DEQ five duplicate samples will be taken in 2005 to assess any annual differences between 2004 and 2005 samples In addition five duplicate samples will be taken in S
24. aken to re calibrate and or repair the equipment If the problem is found to be sampling team error the monitoring team coordinator will review the team s monitoring techniques and ask them to complete another duplicate sample the following month Any limitations on data use will be detailed in both interim and final reports and other documentation as needed The monitoring coordinator and Technical Advisory Committee will review all data resulting from this project to determine if it meets the QA Plan objectives Decisions to accept qualify or reject data will be made by the monitoring coordinator Technical Advisory Committee and DEQ Volunteer Monitoring Coordinator Once data is approved for public release by the monitoring coordinator and Technical Advisory Committee the monitoring coordinator will send an electronic version of the data to the Department of Environmental Quality Lab as part of the equipment loan agreement Project Oversight The monitoring coordinator and the Technical Advisory Committee will be responsible for reviewing the entire monitoring project on a bi annual basis The monitoring coordinator will also receive guidance and advice from state agencies The monitoring coordinator will train all new volunteers before any monitoring activities are done and schedule refresher training sessions as needed All field activities may be reviewed by state agency QA staff at the request of the monitoring coordinator The DEQ Volunt
25. al Habitat Data 1 10 11 12 13 Locate x site using GPS aerial photo and map Scout the area to make sure you won t run into a stream order change upstream of the x site or other condition that would make the stream unsampleable e g stream changes to a marsh goes into an underground pipe etc If one of these situations occurs you ll need to start the reach far enough downstream of x site to avoid this Take several wetted width measurements up and downstream of the x site to determine your average wetted width Record Notice whether this stream seems to have a lot of riffles or few to none so that you can plan your bug sample sites accordingly Multiply your average wetted width by 40 to determine reach length Record Remember minimum reach length is 150 m Starting about 50 feet downstream of the x site and walking upstream on the bank or whatever path is quickest pace off the reach length and either flag or otherwise note where the reach will end Since we are not going to flag transects or macroinvertebrate sites ahead of time this is so you don t collect bug samples outside of the reach Walk about 50 feet downstream of x site don t need to measure just estimate and clip Transect A flag to nearest branch or set on bank Begin collecting your insect samples as you move up through the reach If you feel it s necessary you can walk up and downstream to identify where you want to collect insect s
26. am Make sure the hole on the side of the probe is submerged and doesn t have an air bubble trapped in it LTWC Quality Assurance Project Plan July 2004 2006 27 5 When reading has stabilized record water temperature and conductivity on data sheet It s ok if the last unit for the conductivity reading fluctuates For example if it goes back and forth between 104 5 104 6 and 104 7 just pick the middle value 6 Rinse conductivity probe with distilled water before replacing it in the meter slot 7 Turn machine off and return to bag leaving the cord outside of the bag Make sure the machine doesn t accidentally turn on when you push it into its bag Height from Bridge to Stream Surface This measurement will allow us to estimate stream flow The way it works is that you record the height from a fixed point on the bridge to the surface of the water If the weight on the tape is hanging below the end then measure from the end of the weight If the weight hangs above the end of the tape then measure from the end of the tape Be very precise and be sure to take it from the same point every month Record on your datasheet in feet and 10 of feet e g 16 72 ft Sometimes you might have trouble with the tape flapping in the breeze If this happens try using the bucket to measure the distance Lower it down until the bottom is at the stream surface you can even fill it with a little water if necessary Clamp your fingers on the rope where
27. amples Remember if there s only one good riffle or fastest moving section then you can collect all eight samples from this location If there are more than this spread your samples throughout the reach Collect eight 1 square foot samples as described in DEQ protocol and place each one in the bucket When finished gt Sieve contents of bucket Rinse and discard any large sticks or rocks Make sure there are no insects clinging to them before discarding gt Spoon sediment leaves insects etc from sieve into plastic Nalgene container s Only fill the bottle 4 full Use more than one plastic container if necessary to avoid exceeding the Ya Y2 full guideline gt Top off with alcohol gt Place label s inside jar and tape to outside USE PENCIL ONLY for writing on labels as alcohol dissolves ink Measure water temperature and take several photos that are representative of the reach You can do these two things at any time Just don t forget Go back to Transect A and begin physical habitat survey Divide total reach length by 10 to determine distance between transects At each transect make 5 substrate and depth measurements left bank edge 4 across Y2 way across across right bank edge Estimate Ya 2 and of the way across You do not need to estimate embeddedness At each transect make estimates for riparian vegetation and shade As you walk up the stream between transects gt Measure thalweg depth
28. available from the web page to do common population estimates in the statistical software environment R For further information contact Anthony Tony R Olsen USEPA NHEERL Western Ecology Division 200 S W 35th Street Corvallis OR 97333 Voice 541 754 4790 Fax 541 754 4716 email Olsen Tony epa gov LTWC Quality Assurance Project Plan July 2004 2006 35 Bibliography Diaz Ramos S D L Stevens Jr and A R Olsen 1996 EMAP Statistical Methods Manual EPA 620 R 96 002 U S Environmental Protection Agency Office of Research and Development NHEERL Western Ecology Division Corvallis Oregon Stevens D L Jr 1997 Variable density grid based sampling designs for continuous spatial populations Environmetrics 8 167 95 Stevens D L Jr and Olsen A R 1999 Spatially restricted surveys over time for aquatic resources Journal of Agricultural Biological and Environmental Statistics 4 415 428 Stevens D L Jr and A R Olsen 2003 Variance estimation for spatially balanced samples of environmental resources Environmetrics 14 593 610 Stevens D L Jr and A R Olsen 2004 Spatially balanced sampling of natural resources in the presence of frame imperfections Journal of American Statistical Association 99 262 278 Web Page http www epa gov nheerl arm LTWC Quality Assurance Project Plan July 2004 2006 36 Appendix H Sequence of Measurements for Collecting Macroinvertebrates and Physic
29. ble standards and spiked samples Nitrate Nitrite N Delta Environmental Delta 0 02 to 50 mg L 10 Environmental Watershed Council Laboratories duplicates 0 1 mg L 10 of NIST or 20 traceable standards and spiked samples E coli Delta Environmental Delta 0 to 2419 cells 10 Environmental Watershed Council Laboratories check duplicates 0 5 log that container volume is within 10 of acceptable range and check medium for growth of correct bacteria Representativeness Samples for dissolved oxygen conductivity and turbidity will be collected at or near the center of the stream channel where the water is well mixed and most representative of the ambient conditions Continuous temperature data loggers will be placed in a location that is well mixed and represents the average thermal condition of the stream Comparability This monitoring program will ensure comparability with similar projects in other watersheds by following the standardized sampling protocols and procedures developed by state agencies These protocols are described in detail in the OPSW Water Quality Monitoring Guidebook Delta Environmental performs duplicates on one out of 20 samples or one in each batch of samples if smaller than 20 LTWC Quality Assurance Project Plan July 2004 2006 17 Completeness It is anticipated that samples will be collected from at least 90 of selected sites during all sampling events unless unanticipated w
30. e When sites are replaced the survey design weights are no longer correct and must be adjusted The weight adjustment requires knowing what happened to each site in the base design and the over sample sites EvalStatus is initially set to NotEval to indicate that the site has yet to be evaluated for sampling When a site is evaluated for sampling then the EvalStatus for the site must be changed Recommended codes are EvalStatus Name Meaning Code TS Target Sampled site is a member of the target population and was sampled LD Landowner Denial landowner denied access to the site PB Physical Barrier physical barrier prevented access to the site NT Non Target site is not a member of the target population NN Not Needed site is a member of the over sample and was not evaluated for sampling Other codes Many times useful to have other codes For example rather than use NT may use specific codes indicating why the site was non target Statistical Analysis Any statistical analysis of data must incorporate information about the monitoring survey design In particular when estimates of characteristics for the entire target population are computed the statistical analysis must account for any stratification or unequal probability selection in the design Procedures for doing this are available from the Aquatic Resource Monitoring web page given in the bibliography A statistical analysis library of functions is
31. e Location Storage Time Quality Assurance Project Plan Council office DEQ 10 years OWEB s Oregon Plan for Salmon and Watersheds Council library 10 years Water Quality Monitoring Guidebook methods manual Completed Field Data Sheets for Monthly Monitoring Council office 5 years and Stream Physical Habitat See Appendix E and J for sample data sheets Field Data Notebooks back up record of field data Volunteer s homes 5 years sheets while monitoring Council office when complete Equipment Notebooks records of calibration and Council office w 5 years accuracy checks equipment Continuous Temperature Audit Forms See Appendix Council office 5 years F for example data sheet Laboratory Reports from Delta Environmental Council office 5 years Macroinvertebrate taxonomic report submitted by Council office 10 years contracted taxonomist Final Reports Two year summary of results Council office 10 years Data Management The monitoring coordinator will check all field data sheets for completeness and accuracy at the end of each sampling period Errors will be corrected prior to entering the data into the comprehensive database Unusual results or data recording errors will be noted in the coordinator s logbook so that she can either find an explanation for the results or help the person collecting the data avoid similar mistakes in the future The laboratory technician and Quality Control Director at Delta Environmental will
32. e base design has 25 sites for each panel If it is necessary for a site to be replaced then the lowest ordered SiteID that is part of the oversample of sites identified by OverSamp in variable Panel must be used Subsequent replacement sites continue to be used in the same way Sample Frame Summary The total stream length in the sampling frame 611 1922 km The length by subbasin is Bear Creek Coyote Creek Elk Creek Ferguson Creek 49 59577 116 52424 69 28891 43 59273 Fern Ridge Lower Amazon Lower Long Tom S Spencer Creek 25 99268 51 08396 87 24958 51 18467 Upper Amazon Upper Long Tom 32 47687 84 20281 Length by Strahler Order 0 T 2 3 4 5 25 79639 362 11215 74576637 88 90017 22 23291 37 38424 Length by subbasin and Strahler order 0 al 2 3 4 5 Bear Creek NA 39 13466 4 2838470 6 1772580 NA NA Coyote Creek NA 62 17770 24 4372231 23 1726771 6 73663794 NA Elk Creek NA 37 98250 13 3630709 17 1953916 0 74794950 NA Ferguson Creek 8 3651662 25 15761 10 0699470 NA NA NA Fern Ridge NA 24 39850 0 3853058 NA 1 20887275 NA Fern Ridge Reservoir NA NA NA NA NA NA Lower Amazon 5 0864867 23 10276 6 7859163 16 0577302 NA 0 05107026 Lower Long Tom S 5 7957390 43 78448 0 1131816 0 2230060 NA 37 33316647 LTWC Quality Assurance Project Plan July 2004 2006 33 Spencer Creek 0 9514458 38 70643 4 9903732 6 4880552 0 04836915 NA Upper Long Tom Upper Amazon 5 5975515 21 99732 4 8820052 NA NA NA NA 45 67018 5 4554959 19 5860540 13 49108103 NA
33. eather related events or safety issues prevent sampling Measurements outside range Any data or sample values outside of the expected range for the parameter being measured will be rechecked for validity in the field by the monitoring team member and if necessary he she will re sample Data that continue to be outside expected values will be noted on the field data sheet so that the monitoring coordinator can check the equipment and procedures Duplicate Samples Duplicate quality assurance QA samples for all measurements will be made for at least 10 of sites during each sampling period See Appendix D for the duplicate sampling schedules for field monitoring July 2004 June 2006 The monitoring coordinator will check the continuous temperature loggers for accuracy before and after each field deployment and field audit the probes at the time of deployment and removal If time permits we will field audit the probes during the monitoring season Instrument Equipment Testing Inspection and Maintenance Requirements All field monitoring equipment will be tested for accuracy and or calibrated in accordance with the procedures outlined in the appropriate chapters of the OPSW Water Quality Monitoring Guidebook and the manufacturer user manuals The NIST Traceable Thermometer will be returned to the manufacturer for an annual accuracy check The manufacturer will complete the accuracy check and re certify the thermometer to NIST standards All equi
34. eer Water Quality Monitoring Coordinator will perform data quality audits once a year and any all identified procedural problems will be corrected based on his or her recommendations QAPP Updates and Monitoring Reports The Monitoring Coordinator will submit any revisions or updates to the Council s QAPP to the DEQ Volunteer Monitoring Coordinator and Quality Assurance Officer state agencies for review and or approval This will occur if there are any changes to the monitoring program or procedures Annual presentations of results will be given at Council meetings The monitoring coordinator will be responsible for a final written report due by September 30 2006 These reports will be submitted to the Council Cascade Pacific Resource Conservation and Development Oregon Watershed Enhancement Board DEQ City of Eugene and other interested agencies Reports will include results analysis and interpretation as well as pertinent field observations and QA QC assessments LTWC Quality Assurance Project Plan July 2004 2006 20 References Army Corps of Engineers 1999 Unpublished water quality monitoring data City of Eugene 1999 Unpublished water quality monitoring data Lane Council of Governments 1983 Fern Ridge Clean Lakes Study Unpublished report Tetra Tech Inc 1995 Willamette River Basin Water Quality Study A Summary of Recent Scientific Reports on the Willamette River Report prepared for the Oregon Department of Environmental
35. eessececssecssosecssececsseceecsecsscoeesscceesseceessecsscecsseceesseceecoecssscecssoceeesseceesseesssseessecseessee 31 APPENDIX F QA QC SHEET FOR TEMPERATURE DATALOGGERS eooocccccononccssnocccnancccncrocconsonocccnanccncanocccnsonocos 32 APPENDIX G MACROINVERTEBRATE MONITORING SURVEY DESIGN ssssccsssssccsssssccesscccssssccesssscesess 33 APPENDIX H SEQUENCE OF MEASUREMENTS FOR COLLECTING MACROINVERTEBRATES AND PHYSICAL HABITAT DATA icenic ra aa aa a a a a a a a a a a e 37 APPENDIX I OREGON DEQ BENTHIC MACROINVERTEBRATE PROTOCOL FOR WADEABLE RIVERS AND STREAMS A eris osis asitoke oriei t asirese seso S os neseser iis tei eTe eesi erissa essere assaiar sS 38 APPENDIX J PHYSICAL HABITAT DATA SHEET 00 scccscssssccssscccsssssccssssccccssscccccsscsccssssccscssccccssssscccssecccessnseces 41 APPENDIX K SAMPLE CHAIN OF CUSTODY FORM DELTA ENVIRONMENTAL LABORATORIES 43 LTWC Quality Assurance Project Plan July 2004 2006 2 Project Team Position Responsibility Name Phone E mail Water Quality Monitoring Cindy Thieman 683 2983 cindythiemanO yahoo Coordinator com Monitoring Assistant Lori Quillen 683 6949 Iquillen longtom org DEQ 319 Grant Project Rachel Burr 686 7838 burr rachel O deq state Manager DEQ ext 264 or us Watershed Council Tom Mendes 682 8624 Thomas M Mendes ci e Technical Advisory City of Eugene ugene or us Member
36. emperature directly in the stream and fill the glass DO bottle and turbidity vial from the stream Also be sure and rinse with stream water before filling these bottles If you wade into the stream to collect the samples be sure to hold the containers upstream of you so the bottom sediment that you stir up doesn t get into the sample After water has been collected immediately measure water temperature with conductivity meter and conductivity Measure in the blue bucket or directly in stream if flow is too low for bucket Rinse submerge and fill glass DO bottle from blue bucket or stream Important don t pour water into bottle because this will aerate the sample and give an artificially high DO reading Start DO measurement process Measure air temperature Measure turbidity Measure bridge to water surface Note The most important thing is that water temperature and DO are measured immediately after collecting the stream sample because these parameters change rather quickly once the water is taken out of the stream Instructions for duplicate sampling On the Monitoring Dates sheet your name will be listed next to the date you should do a duplicate You only need to do a duplicate at one site For dissolved oxygen fill both bottles at the same time either from the same bucketful of water or from the stream Add the first two powders to one of the bottles and shake as directed Then add the first two powders to the other bottle
37. eptember 2005 to assess seasonal differences between June and September 2005 samples All samples in 2004 were collected in August and September Because of this relatively narrow sampling window we determined seasonal differences would not be an issue in 2004 An outside lab will identify 500 insects to genus level from each of the composited macroinvertebrate samples These results will be analyzed using a predictive model of stream health i e RIVPAC developed by the DEQ Physical habitat measurements may not be made at all sites due to the time consuming nature of collecting this type of data Our first priority is to collect macroinvertebrates at all 100 sites and duplicates at 10 sites After reviewing the results from the macroinvertebrate analysis we will determine the sites for which physical habitat data is most needed to aid in our assessment of these sub watersheds Methods Monthly Field Monitoring A DEQ led training session on equipment and methods was held in August of 1999 Attendees were given hands on experience in collecting field measurements and collecting samples for dissolved oxygen pH conductivity turbidity and water temperature Several subsequent training sessions were conducted in August and September by the monitoring coordinator for volunteers who were not able to attend the DEQ led training The Monitoring Coordinator will train any new volunteers In addition volunteers receive regular feedback on results and
38. ersample List see Appendix G Volunteers will collect macroinvertebrates according to the Oregon DEQ Benthic Macroinvertebrate Protocol for Wadeable Rivers and Streams detailed in Appendix I Measures of physical habitat were adapted from the EPA Western Pilot Field Operations Manual These adapted methods and datasheet are shown in Appendices H and J respectively Volunteers will return macroinvertebrate samples and datasheets to the monitoring coordinator at the end of each sampling day and the monitoring coordinator will deliver the preserved samples to the taxonomist at the end of the sampling season LTWC Quality Assurance Project Plan July 2004 2006 15 Safety Precautions Monthly to bi monthly sampling will be the normal monitoring schedule unless weather or other environmental conditions create unsafe conditions for field staff If conditions do prevent the field staff from conducting a sampling event they should notify the monitoring coordinator as soon as possible record the current conditions in the project notebook and re schedule the sampling event for the earliest possible date Table 6 Potential Safety Hazards and Precautions Potential Risks Precautions 1 Sampling during high Sample from bridge using bucket on end of rope stream flows 2 Slipping on rocks or Use footwear with felt soles and a stick surveying rod to stabilize other slick surfaces in or yourself near stream 3 Sampling from Wea
39. h Peamouth Longnose dace Leopard dace Speckled dace Mountain sucker Sand roller Northern pikeminnow Redside shiner Largescale sucker Threespine stickleback and a variety of sculpin Currently no fish that spawn in the Long Tom Watershed are on the federal list of Threatened and Endangered Species However Spring Chinook use portions of the lower Long Tom River for winter rearing habitat Historically Oregon Chub inhabited the Watershed and this species is currently listed on the Threatened and Endangered Species List The water quality of streams and lakes is a significant issue in the Long Tom Watershed It affects fish and wildlife such as cutthroat trout and red legged frog and has an impact on human health through activities such as swimming and fishing in Fern Ridge Reservoir and the Long Tom River The Long Tom and several of its tributaries are listed on the Department of Environmental Quality s 303 d list for a variety of parameters including bacteria dissolved oxygen turbidity temperature heavy metals and toxic organic compounds see Appendix A The water quality of the Long Tom also has a significant effect on the Willamette River A 1995 Tetra Tech study identified the Long Tom as one of the five most polluting tributaries to the Willamette Tetra Tech 1995 Recent listings of upper Willamette River salmon and steelhead populations highlight a need to understand and improve all watersheds influencing these populations
40. implement a Phase 2 water quality monitoring program There are several significant differences between Phase 1 from September 1999 June 2003 and Phase 2 July 2004 June 2006 First Phase 2 expands the number of sites for nutrients and E coli in some areas and decreases them in others For example we added four sites in the Ferguson Creek sub watershed between our two original sites because this tributary shows impairment for E coli nitrate and total phosphorus However we stopped collecting these parameters at the original upstream most site on Ferguson Creek FC2 because this location did not show impairment for any of these parameters Second in order to conserve time and money we collect nutrient and bacteria samples every other month Third we stopped measuring dissolved oxygen between December and March because data from the past four years indicate that low dissolved oxygen is not a problem in the winter Fourth we focused our continuous temperature monitoring on identifying tributaries that meet state temperature standards in the summer Fifth we added a macroinvertebrate monitoring program which will characterize the health of stream biota at a sub watershed scale One similarity between Phase 1 and 2 is that we are continuing monthly field monitoring at our 18 baseline sites In overview this program includes I Monthly monitoring of water temperature turbidity and conductivity at 18 baseline sites I Monthly moni
41. ity and pH data follow predictable trends Conductivity levels are higher in the summer especially at downstream sites and lower in the winter Because there is no state standard for this parameter it is difficult to say whether conductivity levels are a problem At our urban and agricultural sites there are distinct spikes in conductivity during the summer months This may relate to fertilizer and other chemical uses in addition to slower flows which concentrate salts in the water pH is lower in the winter and higher in the summer especially in the afternoons when photosynthetic rates are highest pH does not appear to be a significant problem in our basin when compared with the state standard LTWC Quality Assurance Project Plan July 2004 2006 6 Turbidity data show spikes associated with winter storm events At certain sites turbidity levels have exceeded 50 NTU during the winter We will re evaluate our turbidity data when the new turbidity standard becomes finalized Objectives The Long Tom Watershed Council will use the Phase 2 water quality monitoring results to e Support implementation of the Upper Willamette TMDL e Identify tributaries that are contributing to bacteria and nutrient problems in sub watersheds where one or more of these parameters have been identified as consistently not meeting state standards e Initiate cooperative problem solving through the Council s Sub Watershed Enhancement Program by discussing results with
42. l residential Poodle Creek Hwy126 PCT1 NO E coli Forestry livestock 0 9 44 0671 123 4569 agriculture rural residential 3 Site ID s in bold are baseline sites LTWC Quality Assurance Project Plan July 2004 2006 Ferguson Creek Fergsuon Rd FC2 D O Cond Turb Temp Forestry 6 2 44 2509 123 3717 MP 9 Ferguson Creek Territorial FC1 D O Cond Turb Forestry livestock 1 4 44 2474 123 2880 Y Rd Temp NOs TP E coli agriculture rural residential 5 Ferguson Creek bridge on FCA E coli Forestry livestock 2 8 44 2403 123 3148 z private land agriculture rural residential 5 Ferguson Creek Turnbow Rd FCB NO TP E coli Forestry livestock rural 4 6 44 2438 123 3464 Bp residential oa Ferguson Creek Ferguson FCC NO TP E coli Forestry livestock rural 6 2 44 2518 123 3725 Rd residential South Fork Ferguson Cr bridge FCD NO3 TP E coli Forestry livestock rural 0 6 44 2454 123 3800 on private land residential Lower Long Tom Bundy LL1 D O Cond Turb Forestry agriculture 0 8 44 3799 123 2486 Bridge Temp NO3 TP E coli livestock urban rural residential Lower Long Tom Hwy 36 LL2 D O Cond Turb Forestry agriculture 18 2 44 1904 123 2787 Temp NO3 TP E coli livestock urban rural E residential E Lower Long Tom Clear Lake LL3 D O Cond Turb Fore
43. n None Spar Mod Den Ground cover None Spar Mod Den None Spar Mod Den D gt 70 deciduous C gt 70 conifer M mixed N No trees Spar sparse Den dense Mod moderate Ground cover none bare dirt duff sparse some bare dirt duff dbh diameter at breast height LTWC Quality Assurance Project Plan July 2004 2006 42 Appendix K Sample Chain of Custody Form Delta Environmental Laboratories Delta Environmental 36 Irving Rd Phone Chain of Custody Services Inc Eugene 541 689 3177 Oregon FAX Record 97404 541 689 5104 Report Attention Project Company Name ID Address P O Number Phone _ FAX ___ For Lab Use Only Project Number Report Instructions ANALYSES TO BE PERFORMED Collection Sample Sample I D Date Time Grab Composite E coli Nitrate Total P Sample Comments Sampled by print Relinquished by sign Date Time Received by sign Date Time Remarks LTWC Quality Assurance Project Plan July 2004 2006 43
44. ollected at 18 baseline sites within the Watershed Monthly monitoring of dissolved oxygen will occur from April through November at 18 the baseline sites Additional observations that will be documented during each site visit may include recent rainfall weather water color vegetation changes wildlife and recent events in the Watershed that may influence water quality at that site The locations of these original 18 sites were selected with three objectives in mind 1 To characterize water quality in each of the Watershed s sub basins 2 To investigate correlations between water quality and land use 3 To investigate spatial variation of water quality in the Basin i e up to downstream differences LTWC Quality Assurance Project Plan July 2004 2006 7 To accomplish these objectives we selected sampling sites at the mouths of each sub basin at junctures between different land uses and that were distributed in the upper middle and lower portions of the Watershed In addition all 18 sites are monitored once a month in a three day period between 8 00 and 11 00 This helps minimize differences due to time of day or changes in stream conditions Continuous temperature monitoring will be conducted from June through September of 2004 and 2005 using Vemco data loggers The monitoring coordinator and Technical Advisory Committee will determine locations for continuous temperature monitoring Table 3 summarizes the sampling frequency data collec
45. pment has been loaned to the Long Tom Watershed Council by the Oregon DEQ or purchased with funding from Oregon Watershed Enhancement Board The Council will be responsible for maintaining the equipment and restocking all field supplies when necessary Instrument Calibration and Accuracy Checks e The conductivity meter will be calibrated every 3 4 months following the procedure outlined in the user manual e The conductivity meter will be checked for accuracy with secondary standard each day prior to use e The turbidimeter will be re calibrated with formazin standards quarterly e The turbidimeter will be checked for accuracy with secondary standards each day prior to use e There is no calibration for the dissolved oxygen titration However split samples will be performed periodically with DEQ staff to check the accuracy of the field kit e The NIST Traceable Digital Thermometer is calibrated at the factory and will be returned to the DEQ for an accuracy check and re certification once a year e Continuous temperature loggers are factory calibrated and they will be checked for accuracy by the field monitoring team before and after each field deployment e Results of accuracy checks and calibration will be recorded in the appropriate data book for each piece of equipment LTWC Quality Assurance Project Plan July 2004 2006 18 Documentation and Records Document or Record Name and Description Storag
46. r Water Conductivity Number Turbidity Bridge to name temp temp uS cm on D O NTU water surface CE C titrator ft tenths of feet Observations Weather water color flow wildlife changes to stream or riparian zone upstream events over the past month etc Post sampling check list Yes No 1 Have you double checked that all meters are off 2 Did you do an accuracy check for the conductivity meter only need to if you are the first one to use meter that day Did you do an accuracy check for the turbidity meter at your first site 4 Did you measure water temperature and DO immediately after collecting sample 5 Have sampling containers been rinsed and put away 6 Did you enter results in your personal logbook 7 Are all equipment and accessories in box see equipment list Any problems with equipment or sampling LTWC Quality Assurance Project Plan July 2004 2006 31 Appendix F QA QC Sheet for Temperature Dataloggers Project Name Site Name USGS Quad Names and Numbers LASAR Site Description Site Latitude Site Longitude Elevation Temperature Logger ID Date of Battery Installation Data File Name Pre Deployment Temperature Check Thermometer ID Date Thermometer ID Date Low Temp TEMP TEMP High Temp TEMP TEMP TIME MASTER UNIT Difference STATUS TIME MASTER UNIT Difference STATUS AUDIT VALUES
47. r bright orange safety vest provided Once you have collected roadways with heavy the sample do your testing off of the bridge preferably on a side road traffic or driveway with owners permission 4 Spilling chemicals on Use rubber gloves in equipment box and glasses safety goggles clothing skin or eyes when handling powder reagents from dissolved oxygen kit and pH 10 buffer Avoid opening and pouring powders in direct wind When shaking containers hold the container down at your side away from your eyes If chemicals do get on skin or eyes wash them off with water immediately squirt bottle in equip box contact an eye doctor 1f chemicals in get in eyes 5 Accidentally ingesting Call Poison Control Center 1 800 452 7165 chemicals 6 Surface water Wear rubber gloves and or wash hands soap in equipment box after contaminated with toxins completing measurements and before eating or fecal coliform bacteria 7 Getting lost while Bring map compass and GPS unit to site and be comfortable with finding leaving their use If not comfortable with orienteering notify the monitoring macroinvertebrate sites coordinator who will assign you sites that are near a road and easy to find Unless you are leaving to get help for your injured partner stick together while going to and from the site 8 Getting injured while Where protective clothing and proper footwear for hiking in and out traveling to and from of sites B
48. review nutrient and E coli results before mailing results to the monitoring coordinator The monitoring coordinator will also check lab results for completeness and to flag any outliers The data generated from this project will be entered and stored in a computerized database established by the watershed council The database will be compatible with hardware and software used by state water quality agencies Data are available to the public and will be shared with all agencies groups upon request Once the data has been entered in the project database the monitoring coordinator will print a paper copy of the data and proofread it against the original field data sheets Errors in data entry will be corrected at that time Outliers and inconsistencies will be flagged for further review or be discarded Data quality problems will be discussed by the monitoring coordinator and technical advisory committee as they occur and in the final report to data users The paper copy of this data check will be kept on file for at least five years at the Watershed Council office LTWC Quality Assurance Project Plan July 2004 2006 19 After each sampling event determinations of precision completeness and accuracy will be made If data quality does not meet the project s objectives we will determine whether the cause was equipment failure failure to correctly follow methods or other possible factors If the cause is found to be equipment failure steps will be t
49. ring first aid kit along that is included with monitoring macroinvertebrate sites equipment Always go out to the site with your partner and unless you are leaving to get help for your injured partner stick together while going to and from the site Quality Assurance and Quality Control Procedures Measurement Quality Objectives All data will be gathered and handled in accordance with the Oregon Plan for Salmon and Watersheds OPSW Water Quality Monitoring Guidebook The DEQ Data Quality Matrix is shown in Appendix C for reference The type of equipment and methods used in this study are LTWC Quality Assurance Project Plan July 2004 2006 16 sufficient to achieve Level A data The target precision and accuracy levels for A data are listed in Table 7 along with measurement range Quality Control Procedures for Delta Environmental are included in a separate document and will be provided to the DEQ Volunteer Monitoring Coordinator Table 7 Precision and Accuracy Targets Parameter Precision Accuracy Measurement Range Water Temperature 1 5 C 0 5 C 5 to 35 C Conductivity 10 7 of Std Value 0 to 4999 m S cm Turbidity 5 5 of Std Value 0 to 1000 NTU Dissolved Oxygen 0 3 mg L No calibration done 1 to 20 mg l Total phosphorus Delta Environmental Delta 0 02 to 50 mg L 10 Environmental Watershed Council Laboratories duplicates 0 1 mg L 10 of NIST or 20 tracea
50. rural 10 1 44 1824 123 3799 residential Bear Creek Territorial Rd BC1 D O Cond Turb Forestry livestock rural 1 2 44 2143 123 2897 x Temp NOs TP E coli residential 2 Bear Creek Hall Rd BCA NO TP E coli Forestry livestock rural 4 6 44 1852 123 3184 Q residential S Owens Creek Smythe Rd BCT1 NO TP E coli Forestry livestock 1 4 44 2014 123 3312 as Jones Creek Hall Rd BCT2 NO TP E coli Forestry livestock rural 0 6 44 1852 123 3224 residential Battle Creek at Battle Creek Rd BatCr TP E coli Forestry rural residential 0 1 43 9685 123 3206 BatCrRd Coyote Creek Powell Rd CC2 D O Cond Turb Forestry livestock rural 16 4 43 9245 123 2706 Temp E coli residential 5 Tributary of Coyote Creek CC4 D O Cond Turb Forestry Not 43 9200 123 2923 2 Temp NO3 TP avail S Coyote Creek Petzold Rd CC1 D O Cond Turb Forestry livestock 6 35 44 0043 123 2694 5 Temp TP E coli agriculture rural residential Coyote Creek at Battle Creek CC Bat TP E coli Forestry livestock rural 11 3 43 9697 123 3189 Rd CrRd residential Cedar Creek Bishop Rd off EC2 D O Cond Turb Forestry 0 6 44 0679 123 5338 Hwy 126 Temp NO i Elk Creek Vaughan Rd EC1 D O Cond Turb Forestry livestock 0 1 44 0558 123 4515 Q Temp NOs E coli agriculture rural residential Noti Creek Vaughn Rd NCT1 NO E coli Forestry livestock 0 6 44 0558 123 4518 agriculture rura
51. seseseseseseseseseseeeseseseseseseseeesesesesesesesesesescscsessesesesessseseeeseseeees 14 QUALITY ASSURANCE AND QUALITY CONTROL PROCEDURES oocccccconnonccnnnnoconanocconanncnccnoncncanocccnconocccnacccnonos 16 MEASUREMENT QUALITY OBJECTIVES voei ei nereo o ae en aei e e Er ar e aE eatp ariera Eie eae RTE a EON aien aa r ia aii 16 DUPLICATE SAMPLES iia 18 INSTRUMENT EQUIPMENT TESTING INSPECTION AND MAINTENANCE REQUIREMENTS ooccccccconononnnnnonccnonannnnnnnncononannanononos 18 DOCUMENTATION AND RECORDS iia 19 DATA MANAGEMENT e e di 19 PROJECT OVERSIGHT snara a checks Baste Bae bs ENE a ea Sas as Babs AE a a inves a tee ds Na t cs bites ei 20 QAPP UPDATES AND MONITORING REPORTS ka e api a a E S aE E a EEEE DEV eS EAE aa 20 REFERENCES isccsssccscstooscsnccesessensvesstecseeosssssecossovsvccnsesdesdeseviescodsetecsosesseossduededesseceodestecssbusdecescavessoessdecsuvenseetecbesecdescosesdeesssess 21 APPENDIX A 2002 303 D LIST OF WATER QUALITY LIMITED STREAMS IN LONG TOM WATERSHED ETET EE T A S R E A T E E EEE O EEE E TA EESE 22 APPENDIX B SAMPLING ORDER amp EQUIPMENT INSTRUCTION SHEETS esesssecescsecssececsseceessecssoseessoceessee 23 APPENDIX C DEQ DATA QUALITY MATRIX 000 ccccssssccssssscccsscsccssssscccssssccscsscecccsssccccsssaccsessnsecsescsccssseccscsssseces 29 APPENDIX D SAMPLING SCHEDULE AND DUPLICATES sccccsssscssssscccsssscccsssscccssseccscsscesessccccsssecesesssescees 30 APPENDIX E FIELD DATA SHEET eesse
52. streamside landowners e Continue to monitor watershed conditions and verify that data we have collected over the last three years accurately reflects the ecological condition of the Watershed e Evaluate stream health based on the macroinvertebrate community e Monitor effectiveness of restoration and enhancement activities at a sub watershed scale e Educate and involve landowners residents and high school age youth in water quality monitoring and analysis Monitoring Questions 1 For sub watersheds where E coli consistently does not meet state standards do selected tributaries contribute a significant proportion of the bacteria loading or is the loading distributed across the sub watershed 2 For sub watersheds where phosphorus or nitrates consistently do not meet state standards do certain tributaries contribute a significant proportion of the nutrient loading or is the loading distributed across the sub watershed 3 Are correlations between water quality trends and land use consistent with what we ve seen over the past four years 4 Have water quality conditions improved or worsened at a sub watershed and watershed scale over the past 6 years 5 Based on macroinvertebrate assemblages what percentage of stream miles are in good fair or poor condition Study Design Monthly Field Monitoring and Summer time Temperature Monitoring Monthly surface water measurements of temperature conductivity and turbidity will continue to be c
53. stry agriculture 24 44 1214 123 3090 S Rd Temp NO3 TP E coli livestock urban rural E residential gt Lower Long Tom LLA NO3 Forestry agriculture 7 6 44 3129 123 2959 S Monroe Hwy 99 livestock urban rural residential Lower Long Tom Cox Butte LLB NO3 Forestry agriculture 14 3 44 2372 123 2648 Rd livestock urban rural residential Lower Amazon High Pass LA1 D O Cond Turb Agriculture urban 1 4 44 2147 123 2504 Rd Temp NO3 TP E coli 5 3 Lower Amazon Alvadore LAA NO3 TP Agriculture urban 3 5 44 1875 123 2476 3 E Lower Amazon O LAC NO3 TP Agriculture urban 10 8 44 0920 123 2149 lt Bodenhammer Rd Lower Amazon Meadowview LAB NO3 TP E coli Agriculture urban 6 2 44 1499 123 2402 Rd LTWC Quality Assurance Project Plan July 2004 2006 10 Tributary to Spencer Creek SC2 D O Cond Turb Livestock rural residential 0 5 43 9790 123 2079 Summerville Rd Temp E coli 5 Spencer Creek Pine Grove SC1 D O Cond Turb Forestry livestock rural 2 0 43 9924 123 2376 2 Rd Temp TP residential 2 Spencer Creek Lorane Hwy SCA TP Forestry livestock rural 0 7 44 0001 123 2559 av residential Upper Amazon Danebo Ave UA1 D O Cond Turb Urban 14 6 44 0493 123 1777 n Temp NO3 TP E coli 23 a a E Dq on Upper Long Tom Hwy 126 UL1 D O Cond Turb Forestry livestock 35 44 0520 123 3712 S near Venet
54. this should only be done when absolutely necessary Volunteers will dispose of liquid waste from the dissolved oxygen titration in a liquid waste container included with the field equipment kit The monitoring coordinator will then dispose of the liquid waste by putting it down the sink drain at the Council office While pouring the waste down the drain the faucet will be turned on to dilute the concentration of the titrating chemicals Continuous Temperature Monitoring The monitoring coordinator and program assistant will audit deploy and retrieve continuous temperature probes Continuous temperature loggers will be checked for accuracy before and after field deployment according to the procedures outlined in Chapter 6 of the OWEB Water Quality Monitoring Guidebook In addition the field installation procedures described in Chapter 6 will be followed Loggers will be set to record a data point once an hour At the time of logger deployment and removal the monitoring coordinator will record stream temperature using a NIST traceable thermometer Additional stream temperature audits may be collected throughout the summer The procedure for conducting a field audit on continuous temperature loggers is described in the section Field Checking Instrument Performance of Chapter 6 of the OWEB Monitoring Guidebook An accuracy and audit form will be maintained for each logger for recording the results of the accuracy checks and field audits and submitted to D
55. tor and general method for each parameter Table 3 Summary of Baseline Field Monitoring and Continuous Temperature Sites Frequency responsibility meter 3 September dataloggers meter for flow rating November surface from fixed 1 Bridge height Monthly April Monitoring team Measure to water point on bridge meter Nutrient and Bacteria Sampling In our Phase 1 program we collected nitrate total phosphorus and E coli at the 18 baseline sites once month After evaluating results from four years of data we concluded that we needed better spatial resolution for bacteria and nutrient concentrations in some areas and that we could stop sampling for some or all of these parameters in other areas As a result we now have 33 sites where we collect nitrate total phosphorus and or E coli This will allow us to better determine the source of these pollutants and develop solutions with willing landowners Table 4 lists the monitoring sites where we collect monthly field data i e D O conductivity turbidity temperature and bi monthly grab samples for nitrate NO3 total phosphorus TP and or E coli LTWC Quality Assurance Project Plan July 2004 2006 8 Table 4 Site Descriptions Parameters and Locations Sub Site Description Site ID Parameters Predominant Upstream River Lat Long WS Land Use Mile Bear Creek Templeton Rd BC2 D O Cond Turb Temp Forestry livestock
56. toring of dissolved oxygen from April through November at 18 baseline sites HI Continuous temperature monitoring at up to 34 sites IV Grab samples for E coli nitrate and or total phosphorus on a bi monthly basis at 33 sites V Macroinvertebrate sampling at 100 randomly selected sites in each sub watershed This Quality Assurance Project Plan describes the Program s objectives study design methods and quality control assurance procedures Watershed Overview The Long Tom River Watershed drains 410 square miles of land at the southern end of the Willamette Valley The headwaters of the Upper Long Tom originate on the eastern side of the Coast Range and flow south through forested hills and small farms until reaching Noti where the river veers east near its confluence with Elk and Noti Creek Coyote Creek which drains the southern portion of the basin and Amazon Creek which drains the eastern portion both merge with the Upper Long Tom near what is now Fern Ridge Reservoir The Lower Long Tom spills out the north end of the reservoir and flows approximately 25 miles before joining the Willamette River The Watershed contains four ecoregions which will each require somewhat different water quality goals and management strategies The Mid Coastal Sedimentary and Valley Foothills ecoregions cover the steeper upland areas of the watershed Near headwaters stream channels are confined within steep narrow valleys becoming more sinuous downs
57. tream where the valleys widen The underlying geology is mostly sedimentary with some basalt in the Valley Foothills region The combination of soft sedimentary rock and relatively high precipitation rates in these regions contributes to higher erosion rates Natural vegetation includes western hemlock western red cedar Douglas fir and red alder LTWC Quality Assurance Project Plan July 2004 2006 4 The Prairie Terrace ecoregion covers most of the low gradient valley lands except for a small portion along the Lower Long Tom River which is part of the Willamette River and Tributaries Gallery Forest ecoregion Unmodified streams in these regions cut a sinuous path across the valley floor Many streams have been channelized in order to protect farms homes and businesses from flooding In either case streams are often deeply entrenched in the thick sedimentary clay soils deposited by the Missoula floods thousands of years ago The natural vegetation within the Prairie Terraces region is Oregon white oak Oregon ash Douglas fir and prairie whereas the Willamette River Gallery Forest contains large stands of cottonwood alder Oregon ash bigleaf maple and Douglas fir The Long Tom Watershed is home to a variety of fish amphibians birds and invertebrates that rely on the network of streams lakes and wetlands in the Basin Native fish species include White sturgeon Cutthroat trout Mountain whitefish Pacific lamprey Western brook lamprey Chiselmout
58. uality Assurance Project Plan July 2004 2006 39 Inspect the benthos in a 1 ft X 1ft area approximately as wide as the kick net of stream bottom directly in front of the net for any large organisms such as mussels Pick these and place in the sieve bucket Carefully rub by hand all substrate larger than five centimeters golf ball size and larger in front of the net to dislodge any clinging macroinvertebrates Then with a small scrub brush dislodge organisms still clinging to the larger substrate particles After rubbing place the substrate outside of the sample plot Hand scrubbing is recommended prior to using the brush to prevent damage from occurring to fragile macroinvertebrate specimens Also be gentle with the brush so as not to chew up the macroinvertebrates Thoroughly disturb the remaining substrate in the 1 ft 1ft area with your hands or feet for 1 min to a depth of five to ten centimeters NOTE Collecting a sample in slow moving water is a little more difficult It may involve pulling the net through the water as the substrate is disturbed to capture suspended organisms 6 yl After the sample is collected and the net removed return the large substrate to the sample plot The contents of the net are placed in a bucket and the sampling procedure is repeated for that habitat type Always sample downstream to upstream All kick samples for the same reach are composited in the bucket Large organic material and rocks
59. ugene sampling period not specified LCOG 1981 82 LCOG 1981 84 DEQ 2000 LCOG 1981 82 LCOG 1981 82 LCOG 1981 84 LCOG 1981 82 City of Eugene sampling period not specified DEQ 1986 95 DEQ 1986 95 Season Year round Year round Year round Year round Year round Year round Year round Year round Year round Year round Spr Sum Fall Summer None specified Fall Wint Spr Spr Sum Fall Year around None specified Summer Fall Wint Spr 22 Appendix B Sampling Order amp Equipment Instruction Sheets LTWC Quality Assurance Project Plan July 2004 2006 23 Sampling order 1 2 If possible before going out in the field you should complete your accuracy checks on the conductivity and turbidity meters See instructions for each on the respective equipment instruction sheets If you are not the first person using the equipment that day then you only need to do an accuracy check on the turbidity meter Collect sample in blue bucket from bridge or directly from stream if too shallow for bucket To use the bucket lower it near the center of the channel where the water is well mixed to about 3 below the surface of the water If stream depth is less than 6 estimate then lower bucket about 1 2 way between the surface and bottom of stream Make sure you rinse the bucket with stream water before filling the bucket If stream depth is too shallow to use the bucket then measure conductivity and t

Quality Assurance 2 April 2005

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