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Microliter Flow M.P.C Modular Pump Component User`s Manual
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1. Connection 3 P8 1 P8 2 P7 1 P7 2 P7 3 P7 4 P7 5 P7 6 70 2225 1 Main Unit 2 Universal inpu 100 250VAC 50 60 Hz 18 watt power supply 3 Line Cord 4 RS 232 Communication cable 5 Power Connection Cable 6 User documentation 7 6 oz tube of grease Publication 5417 001 REV B www harvardapparatus com Installation Location Requirements for the Syringe Pump 3 125 125 typ 7998 1 e A sturdy level clean and a dry surface v H ne D m1 i Minimum of one inch 2 5cm clearance around the pump 3438 87 31 gt Appropriate environmental Enel Cur OUI conditions L 6625 i m P 16828 Mount into an appropriately M rated enclosure or cabinet Required clearance below mounting panel 2 00 bcm 1 Dimensions Mounti ol For ta Md screw Le 2 2 al inches cm 7303 Loading the Syringe 1 Release the syringe pusher 4 by pressing the bronze button 3 on the side of the pusher While holding the bronze button in slide the pusher to the right Raise the spring loaded syringe retainer 1 and swing it out of the way Lay the loaded syringe in the V shaped holder Swing the retainer so it holds the syringe in place Move the pusher so it makes contact with the syringe plunger N O O WN Adjust pusher block thumbscrews amp bracket 2 until the Syringe plunger is completely captured 8 The limit switches can be easily adjusted by
2. 72 2478 1140 352 Product 1 8m 6 ft motor encoder extension cable 1 8m 6 ft limit switch extension cable 0 6m 2 ft RJ11 daisy chain cable 1 8m 6 ft RJ11 daisy chain cable 1 0 amp E stop cable w LED www harvardapparatus com 2uouoduuo3 duung 4eJ npojAl mojj 4931 JO4921JN SN Peseddy paeAaeH Declaration of Conformity In Accordance with ISO IEC Guide 22 and EN 45014 Manufacturer HarvardApparatus Inc 84 October Hill Rd Holliston MA 01746 1388 U S A Phone 508 893 8999 We herewith declare that the following product Product Name Micro liter Flow Modular Pump Component Model No 70 2220 70 2225 To which this declaration relates is in conformity with the applicable EC Directives harmonized standards and other normative requirements Application of Council Directive s 89 336 EEC Standard s to which Conformity is Declared Emissions Immunity EN 61326 1 2005 EN 61000 4 2 EN 61000 4 3 EN 61000 4 4 EN 61000 4 5 EN 61000 4 6 EN 61000 4 11 CISPR 11 2004 Class A EMC and Safety compliance were evaluated by Intertek ETL Semko Reference test report file numbers 3125838LTT 001 and 3125828LTT 003 Signature us Ce HARVARD VP Engineering Operations 2 ca Position APPARATUS
3. Use Appropriate Power Supply Use only an approved power supply Be sure to observe proper polarity of connections when hooking up DC supply voltage CAUTION FAILURE TO MAINTAIN POLARITY MAY RESULT IN DAMAGE TO THE UNIT AND WILL VOID THE WARRANTY 12 30Vdc positive PlN 1 on Pin Header P8 12 30Vdc return PIN 2 on Pin Header P8 Ground the Product This product is grounded through a ground stud located on the under side of the base plate To avoid electric shock the grounding conductor must be con nected to earth ground Before making any connections to the input or output terminals of the product ensure that the product is properly grounded Make Proper Connections Make sure all connections are made properly and securely Any signal wire connections to the unit must be no longer than 3 meters Orient Equipment Properly Do not position the equipment such that it is difficult to reach the discon necting device Observe all Terminal Ratings Review the operating manual to learn the ratings on all connections Avoid Exposed Circuitry Do not touch any electronic circuitry inside of the product Do Not Operate with Suspected Failures If damage is suspected on or to the product do not operate the product Contact qualified service personnel to perform inspection Avoid Pinch Hazards Pinch hazards may exist between the pusher block and the end block Avoid placing fingers between these points while the pump is running
4. pulse to a logic low toggles the pump between the RUN and STOP states EXT_ENABL This is an active low TTL level input pulled up to 5V through a 10K ohm resistor It is ESD protected through a TVS device and filtered with a 0 1uF capacitor to ground A transition from logic high to logic low causes the processor to reset to its default state and enables the motor drive A transi tion from logic low to logic high disables the motor drive through hardware independent of firmware This input may be used for an emergency stop function To enable pump the unit or connector P7 pin 3 must be connected to ground Outputs RUN_IND This is an active low output driven by two 74HCT14 inverters in parallel An on board resistor may be placed in series with this output to provide current limiting The default resistor value is zero ohms A logic low indicates RUN A logic high indicates STOP Indicators Power on LED When illuminated indicates that board is powered on and 5VDC supply is operating RUN LED When illuminated indicates that pump is running When extinguished indicates pump is stopped Publication 5417 001 REV B www harvardapparatus com Appendix B Syringe Inside Diameter Size 10 pl 25 50 100 250 500 1000 Unimetrics Series 4000 amp 5000 Diameter 0 46 mm 0 729 1 031 1 460 2 300 3 260 4 610 Scientific Glass Engineering Size 25 ul 50 100 250 500 1ml 100 250 500 1m
5. L Diameter 0 73 mm 1 03 1 46 2 30 3 26 4 61 Hamilton Microliter Series Gastight Diameter 0 103 mm 0 1457 0 206 0 3257 0 460 0 729 1 031 1 46 23 3 26 4 61 www harvardapparatus com 2uouoduuo3 duung 4eJ npojAl mojj 4931 JO4921JN SN Peseddy paeAaeH E Harvard Apparatus Mloicroliter Flow Modular Pump Component Appendix C Flow Rates nominal nominal syringe diameter ul hr ul min ml min size mm min max min max i min max 05g 0 103 0 001 39 840 0 001 0 664 0 001 0 040 0 000 0 000 iu 0 150 0 002 84 480 0 001 1 408 0 001 0 085 0 000 0 001 2y 0 210 0 003 165 601 0 001 2 760 0 001 0 166 0 001 0 003 oM 0 330 0 007 408 962 0 001 6 816 0 001 0 409 0 001 0 007 10 p 0 460 0 013 794 581 0 001 13 243 0 001 0 795 0 001 0 013 25 u 0 730 0 033 1999 999 0 001 33 352 0 001 2 001 0 001 0 033 504 1 030 0 066 1999 999 0 001 66 397 0 001 3 984 0 001 0 066 100 1 460 0 131 1999 999 0 002 133 407 0 001 8 004 0 001 0 133 250 2 300 0 325 1999 999 0 005 331 077 0 001 19 864 0 001 0 331 500p 3 260 0 653 1999 999 0 012 665 133 0 001 39 907 0 001 0 665 1ml 4 610 1 306 1999 999 0 022 1330 073 0 001 79 804 0 001 1 330 Publication 5417 001 REV B www harvardapparatus com Appendix D Serial Communication Commands Queries and Respon
6. Microliter Flow M P C Modular Pump Component User s Manual Catalog 70 2220 amp 70 2225 Va HARVARD APPARATUS 5417 002 REV B WEEE RoHS Compliance Statement EU Directives WEEE and RoHS To Our Valued Customers We are committed to being a good corporate citizen As part of that commitment we strive to maintain an environmentally conscious manufacturing operation The European Union EU has enacted two Directives the first on product recycling Waste Electrical and Electronic Equipment WEEE and the second limiting the use of certain substances Restriction on the use of Hazardous Substances RoHS Over time these Directives will be implemented in the national laws of each EU Member State Once the final national regulations have been put into place recycling will be offered for our products which are within the scope of the WEEE Directive Products falling under the scope of the WEEE Directive available for sale after August 13 2005 will be identified with a wheelie bin symbol Two Categories of products covered by the WEEE Directive are currently exempt from the RoHS Directive Category 8 medical devices with the exception of implanted or infected products and Category 9 monitoring and control instruments Most of our products fall into either Category 8 or 9 and are currently exempt from the RoHS Directive We will continue to monitor the application of the RoHS Directive to its products and will comp
7. PC or other controller and the internal microprocessor drives a preci sion stepper motor to produce accurate fluid flow This unit is designed to operate inside an appropriately rated fire electrical mechanical enclosure or cabinet Features Two Modes of Operation Constant Flow Rate and Volume Dispense The uL Modular Syringe Pump Component will operate continuously in RATE mode or accurately dispense a specific amount of fluid in VOLUME mode When starting the pump RATE mode will be the default mode To operate in Volume mode set a target volume and the pump will change modes to suit desired operation Infuse amp Withdraw Limit Switches Infuse and withdraw limit switches are located on brackets attached to the main mounting plate When the pusher block contacts either limit switch pusher block movement is stopped Take care in adjusting the infuse limit Switch position so that the syringe plunger does not bottom out in the syringe www harvardapparatus com 2uouoduuo3 dung sejnpoy mojj 4931 JOJ921JN SN Peseddy paeAaeH Harvard Apparatus Mlicroliter Flow Modular Pump Component Input Connections Connector Pin outs Input Power Power Connection Return Digital 1 0 Run IND Connection vcc EXT ENABLE Ground Run Stop Ground Packing List 70 2220 1 Main Unit 2 RS 232 Communications cable 3 User documentation 4 6 oz tube of grease withdraw Limit switch Connection P4 Infuse Limit Switch
8. Procedures which could result in injury shall only be carried out by opera tors who have been warned of the potential hazards and have received adequate training in carrying out the procedures in the safest possible manner www harvardapparatus com 2uouoduuo3 dung sejnpoyy mojj 4931JOJ921JN SN Peseddy paeAaeH E Harvard Apparatus Mlicroliter Flow Modular Pump Component G eneral Safety Summary Place Product in Proper Environment Environmental Conditions Indoor use only Temperature 5 C to 40 C 40 F to 104 F Humidity 20 to 80 RH Well Ventilated Room Altitude up to 2000 m DC Voltage Fluctuation not to Exceed 5 of Nominal Transient Over voltage Category Il Pump is Rated Pollution Degree Observe all Warning Labels on Product Read all labels on product to ensure proper usage A CAUTION Protective Ground Refer to Manual Terminal CAUTION CAUTION Pinch Hazard ESD Sensitive Device A Pub CAUTION Pinch Hazard lication 5417 001 REV B www harvardapparatus com Introduction Theory of Operation The uL Modular Syringe Pump Component is designed as a highly precise single syringe infusion withdrawal pump module capable of low to moderate back pressures The module includes a mechanism hardware and software Typically the uL Modular Syringe Pump Component holds one syringe of most makes from 0 5 ul to 1ml The diameter of the syringe is entered via your
9. d Rate Data Bits Parity Stop Bits Flow Cntrl Emulation ASCII Setup 9600 Echo typed characters locally 8 Line delay 0 None Character delay 0 2 Wrap lines None Auto Detect www harvardapparatus com yu uoduuoS dung 4PejJnpojA l mojj 4931JO421JN Sn3eueddy pieAieH _ Harvard Apparatus Microliter Flow Modular Pump Component HYPER TERMINAL Terminal Emulation Software You may want to save the setup information under a descriptive filename 4 At the PC launch HyperTerminal with the above setup specifications if it is not already running Type VER at the PC keyboard and verify that the pump module s version is displayed at the PC terminal 5 Type RUN to start the pump type STP to stop the pump After starting the pump should be displayed indicating pump is infusing After stopping the pump should be displayed Publication 5417 001 REV B www harvardapparatus com Appendix A Specifications Accuracy 0 35 Reproducibility 0 1 Number of Syringes One Syringe Sizes 0 5uL min 1 mL max Flow Rate Minimum 0 001 uL hr for a 0 5mL syringe Maximum 1330 L min for a 1mL syringe Linear Force 7lbs peak min Adjustable Drive Motor 0 9 Stepper Control Constant Current Drive 0 50A peak per phase 1 4 1 16 stepping Drive Ratio 1 1 Leadscrew Pitch 48 threads per inch 1 4 48 Encode Optical 100 lin
10. ds will not be accepted for return unless an RMA returned materials authorization number has been issued by our customer service department The customer is responsible for shipping charges Please allow a reasonable period of time for completion of repairs replacement and return If the unit is replaced the replacement unit is covered only for the remainder of the original warranty period dating from the purchase of the original device This warranty gives you specific rights and you may also have other rights which vary from state to state Repair Facilities and Parts Harvard Apparatus stocks replacement and repair parts When ordering please describe parts as completely as possible preferably using our part numbers If practical enclose a sample or drawing We offer a complete reconditioning service CAUTION CAUTION This pump is not registered with NOT FOR CLINICAL USE the FDA and is not for clinical use ON HUMAN PATIENTS on human or veterinary patients It is intended for research use only Publication 5417 001 REV B www harvardapparatus com General Safety Summary Please read the following safety precautions to ensure proper use of your modular syringe pump To avoid potential hazards and product damage use this product only as instructed in this manual If the equipment is used in a manner not specified by the manufacturer the protection provided by the equipment may be impaired To Prevent Hazard or Injury
11. e for stall detection Step Rate Minimum 3 8 sec step Maximum 250 uLsec step Pusher Travel Rate Minimum 0 001 mm min Maximum 83mm min Display None Keypad None Interface RS 232 Connectors Power 2 pin Header 1 centers RS 232 RJ 11 Phone jack Power 12 to 30VDC 5 12W max Dimensions 7 25 X 3 625 X 4 50 L X W X H 18 41cm X 9 20cm X 11 43cm Mounting Dimensions 6 875 X 3 25 Mounting holes for 6 6 screws 17 46cm X 8 25cm Ground Stud 6 32 Stud Weight 1 75 Ibs 0 8kg Environmental Operating Temperature 5 to 40 C natural convection cooling Humidity 20 to 80 RH non condensing Storage Temperature 30 to 45 C Humidity 10 to 90 RH non condensing Pollution Degree Class Compliance Lead Free ROHS Compliant www harvardapparatus com 2uouoduuo3 duung ae jnpojN mojj 4931 JOJ921JN SN Peseddy paeAaeH 7 Harvard Apparatus Microliter Flow Modular Pump Component Appendix A Specifications The pump is set to the following default parameters on power up and after an external enable command Default Settings Syringe Diameter 2 300 mm Rate 3 000 ul min Range ul min Force 100 Address 00 Baud Rate 9600 I O Specifications Inputs RUN STOP This is an active low TTL level input pulled up to 5V through a 10K ohm resistor It is ESD protected through a TVS device and filtered with a 0 1uF capacitor to ground Each
12. e and range units are milliliters per minute ULH number Set infuse flow rate and range units are microliters per hour ULHW number Set withdraw flow rate and range units are microliters per hour www harvardapparatus com 2uouoduuo3 dung sejnpoyy mojj 4931JO491 JN SN Peseddy paeAaeH _ Harvard Apparatus Microliter Flow Modular Pump Component Appendix D Serial Communication Commands with Numbers continued FOR number Force setting as percentage number can be 0 100 MLH number Set infuse flow rate and range units are milliliters per hour MLHW number Set withdraw flow rate and range units are milliliters per hour MLT number Set target infusion volume units are ml or ul depending on range MLTW number Set target withdraw volume units are ml or ul depending on range Numbers can be between 0 and 1999 Leading zeros and trailing decimal point are optional Any number of digits to the right of the decimal point may be transmitted The number will be rounded For rate numbers only use X instead of a number to get maximum rate Oueries DIA Returns diameter value units in mm RAT Returns infusion rate value set in current range units RAT W Returns withdraw rate value set in current range units VOL Returns current accumulated infused volume units in ml or ul depending on range VOLW Returns current accumulated withdraw volume units in ml or ul depending on range VER Returns model and version numb
13. er Flow Modular Pump Component General Information Serial Number All inquires concerning our product should refer to the serial number of the unit Serial numbers are located on the underside of the mounting plate Calibration All syringe pumps are designed and manufactured to meet their perform ance specifications at all rated voltages and frequencies Warranty Harvard Apparatus warranties this instrument for a period of two years from date of purchase At its option Harvard Apparatus will repair or replace the unit if it is found to be defective as to workmanship or material This warranty does not extend to damage resulting from misuse neglect or abuse normal wear and tear or accident This warranty extends only to the original customer purchaser IN NO EVENT SHALL HARVARD APPARATUS BE LIABLE FOR INCIDENTAL OR CONSEQUENTIAL DAMAGES Some states do not allow exclusion or limitation of incidental or consequential damages so the above limitation or exclusion may not apply to you THERE ARE NO IMPLIED WARRANTIES OF MERCHANTABILITY FITNESS FOR A PARTICULAR USE OR OF ANY OTHER NATURE Some states do not allow this limitation on an implied warranty so the above limitation may not apply to you If a defect arises within the two year warranty period promptly contact Harvard Apparatus Inc 84 October Hill Road Holliston Massachusetts 01746 1388 using our toll free number 1 800 272 2775 Outside the U S call 508 893 8999 Goo
14. er of firmware TAR Returns target infuse volume units in ml or ul depending on range TARW Returns target withdraw volume units in ml or Hl depending on range FRC Returns force setting and percent Value format nnnn nnn The returned value is an 8 character string with leading zeros converted to SP characters ASCII 32 decimal The fifth character is a decimal point ASCII 46 decimal Oueries with String Response RNG Returns infuse range message character string either ML H ML M UL H UL M RNG W Returns withdraw range message character string either ML H ML M UL H UL M EMG Returns emergency stop input status True False True Emergency Stop LIM Returns W or false indicating limit switch actuation status Error responses CR LF CR LF prompt Unrecognized command CRLF OOR CRLF prompt Entered value is out of range Publication 5417 001 REV B www harvardapparatus com Appendix D Serial Communication Note Adjustable force settings 7 Ibs min o 2 o LL 0 20 40 60 80 100 Force Current Due to differences in mechanical drag forces we do not recommend operating the pump below 40 force level TRANS GND REC GND GND TRANS GND REC OUT IN Chain Host Connection to Computer Additional Pump s Connection www harvardapparatus com 2uouoduuo3 dung 4eJ npojAl mojj 4931JO4921 JN SN Peseddy paeAaeH Harvard Apparatus Mlicroliter Flow Modular Pump Component Ap
15. loosening the 2 nuts not shown sliding the switch to the desired position and retightening the nuts into place Y dd 9 www harvardapparatus com 2uouoduuo3 dung sejnpoyy mojj 4931JOJ921JN sm3e24eddy paeAaeH 4 Harvard Apparatus Mlicroliter Flow Modular Pump Component Operation Getting Started Plug one end of the power cable into P8 connection on board Connect the other end of the cable into your power supply PC Connect communica tion cable from PC to J1 connector Refer to page 8 of this manual to refer ence the board connections Working With the Pump The safest way to use the uL Modular Syringe Pump Component is in the volume dispense mode The pump will automatically stop when target vol ume is dispensed Enable must be tied to ground Hardwire P7 3 to P7 4 or connect through switch Check Syringe Often The uL Modular Syringe Pump Component will shut itself off when the syringe is empty or is otherwise overloaded Although this presents no haz ard to the user or the pump it is prudent to check the syringe from time to time Maintenance Keep the uL Modular Syringe Pump Component clean and dry Avoid liq uid spills that may find their way into the electronics A small tube of grease is provided for periodic lubrication of the lead screw It is important to keep the lead screw clean and lubricated To clean the exterior surfaces above the base plate use a lint free cloth to
16. ly with any changes as they apply Do Not Dispose Product with Municipal Waste Special Collection Disposal Required Table of Contents Subject Page No General Information Warranty and Repairs 2 General Safety Summary 3 4 Introduction Theory of Operatioll uu eire ce teva YDD yd 5 gung E 5 Input Connecti ONS eesosas en bone ai anaE 6 Packing EISE eL Estes erbe sees 6 Installation Initial Setup amp Location Requirements 7 Loading the Syringe 7 Operation Getting Started ouod ii u u 8 Working with the pump 8 Cheek Syringe soiis esisiini inia ae ak e Rr y EEG 8 Maintenance u RE 8 Protecting Small Fragile Syringes 8 Hyper Terminal ci aa sn Le Dr t tenen 9 10 Appendices A Specifications uuu SS 11 Inputs eret ede Gat Aka 12 e Iiic u S 12 B Table of Popular Syringe Diameters 13 C Flow Rates ni uu REID uu 14 D Serial Communications Commands Queries and Responses 15 17 E Optional Daisy Chaining 18 F Accessories luu u ER Deren eyes erus 19 www harvardapparatus com 2uouoduuo3 dung 4AeInpo jlAil mojj 4931JOJ921JN SN Peseddy paeAaeH Harvard Apparatus Microlit
17. pendix E Optional Daisy Chaining The RS 232 Connection to the first board in the daisy chain is made through the 9 pin D Sub connector or RJ 11 input J1 Use an appropriate adapter to connect a 9 pin or 25 pin D Sub at a PC to the RJ 11 input A straight through RJ 11 to RJ 11 cable is used to connect from the RJ 11 output J2 of the first board to the input of the next board in the daisy chain Set each board in the chain to be at a unique address 00 to 15 by setting the DIP switches SW1 as follows Position Value 4t f H H II oo t Addr 00 Addr 01 Addr 02 Pos 1 OFF Pos I ON Pos 1 OFF Pos 2 OFF Pos2 OFF Pos2 ON Pos 3 OFF Pos 3 OFF Pos3 OFF Pos 4 OFF Pos4 OFF Pos 4 OFF SW1 ON SWI ON SWI ON Addr 03 Addr 08 Addr 15 Pos 1 ON Pos 1 OFF Pos 1 ON Pos 2 ON Pos2 OFF Pos2 ON Pos3 OFF Pos3 OFF Pos3 ON Pos4 OFF Pos4 ON Pos 4 ON Fig 1 5 Examples of the 16 Possible Address Configuration Settings To communicate with each board in the daisy chain prefix commands and queries with the address Addresses must be two digits i e 00 to 15 These addresses are based on the principle of binary notation Add up your address value by moving the appropriate switch to the on position Pump must be power cycled or reset for address change to be recognized Publication 5417 001 REV B www harvardapparatus com Appendix F Optional Accessories Catalog No 70 2231 70 2232 55 7760
18. remove loose dust For more efficient cleaning use a soft cloth dampened with water or an aqueous solution of 75 isopropyl alcohol If the pump does not work properly contact Harvard Apparatus for appropriate instructions Protecting Small Fragile Syringes The uL Modular Syringe Pump Component will hold micro liter size syringes down to 0 5jl size These small syringes have fine wire plungers that may be damaged if allowed to bottom out The limit switches for the uL Modular Syringe Pump Component can be adjusted by loosening the mounting hardware which attaches the switch to the base plate bracket moving the switch to the desired location and re tightening the switch mounting hardware Publication 5417 001 REV B www harvardapparatus com HYPER TERMINAL Terminal Emulation Software Remote Control via the RS 232 Interface Using HYPERTERMINAL Normally included with most Windows operating systems Milliliter Modular Syringe Pump Component 1 Connect the RS 232 cable between the uL Modular Syringe Pump Component RS 232 IN port and a PC s serial port 2 On the PC running a Microsoft Windows Operating System select START PROGRAMS ACCESSORIES HYPERTERMINAL HYPERTERMINAL to start the HyperTerminal application If HyperTerminal is not available install it from the Microsoft Windows Operating System Install disks or CD ROM 3 Setup the appropriate COMPORT for the following Configure Bau
19. ses After each transmission to the pump terminating with a CR character ASCII 13 the pump enters remote mode and responds with the character sequence CR LF prompt The prompt characters indicate the status of the pump as follows prompt meaning ASCII code Stopped ASCII 58 decimal gt Running forward ASCII 62 decimal lt Running reverse ASCII 60 decimal Stalled ASCII 42 decimal Infuse limit switch actuated ASCII 42 decimal ASCII 73 decimal W Withdraw limit switch actuated ASCII 42 decimal ASCII 87 decimal D Disabled by emergency stop ASCII 42 decimal ASCII 68 decimal PT Target volume reached ASCII 42 decimal ASCII 68 decimal Serial Commands and Their Meanings Commands RUN Start infuse forward direction RUNW Starts withdraw reverse direction STP Stop motor CLV Clears infuse volume accumulator to zero CLVW Clears withdraw volume accumulatorto zero CLT Clears target infuse volume to zero dispense disabled CLTW Clears target withdraw volume to zero REV Reverses pumping direction Commands with Numbers Must have space before number MMD _ number Set syringe diameter units are mm Rate is set to zero after MMD command ULM number Set infuse flow rate and range units are microliters per minute ULMW number Set withdraw flow rate and range units are microliters per minute MLM number Set infuse flow rate and range units are milliliters per minute MLMW number Set withdraw flow rat
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