accuFet™ XL Solid-State pH Probe Series User Manual
Contents
1. cap Leaving a drop of deionized or distilled water in the protective tube provides a humid environment avoids dehydration of the reference electrode and therefore allows for faster start up times Note When used in samples containing small hard particles e g pigments titanium oxides silicates etc first rinse the probe with ample water before using the brush OPERATING TIPS Tip 1 To prepare saturated KCl potassium chloride solution Add KCl granules to distilled water until no more KCI will dissolve Adding 38 grams of KCI to 100 ml water is sufficient Let this stand for at least two hours and decant the clear solution Now you have saturated KCl Tip 2 To ensure correct measurement values samples or buffers need to be mixed well This may be done by a magnetic stirrer or by stirring with the probe for at least 5 seconds Stop stirring and record results when the read out is stable Tip 3 Proteins fats and oils may be removed by scrubbing in a solution of Terg A Zyme Alconox company a pepsin solution 68X068047 rev 0 05 2004 3 8 accuFet XL Solid State Instruction Manual or a similar product Afterwards rinse thoroughly with deionized or distilled water Do not use hydrofluoric acid acetone MEK or similar agents to clean the probe Tip 4 When testing in direct sunlight or on a bright reflecting surface please use brown opaque or shielded sample containers Very bright light might influence the performa2. must be aqueous solutions or semi solids and compatible with the probes wetted materials 68X068047 rev 0 05 2004 2 8 accuFet XL Solid State Instruction Manual 4 1 2 3 4 5 6 7 8 5 DAILY USE Remove and save the protection tube from the probe prior to use Prior to daily use scrub the probe tip with a soft bristled toothbrush and water to remove possible residues Use some mild detergent if required Reference gel may be observed as a viscous material on the tip of the probe Some gel seepage from a new probe is normal and will not affect the longevity or performance of the probe Rinse with deionized or distilled water Soak the probe in pH 7 00 buffer or pH 4 00 buffer with the meter ON in any mode prior to calibration for at least 10 minutes If the probe has not been used for two or more days and stored dry clean as above place in saturated KCI solution See Tip 1 for 10 to 15 minutes then in pH 7 00 or pH 4 00 buffer as described above For maximum stability and accuracy perform the calibration sequence twice To store the probe clean it thoroughly with water and a soft bristled toothbrush again a mild detergent may be used and rinse with deionized or distilled water Replace the protective tube When doing so slide the screw cap onto the probe body followed by the sealing O ring Slide cap and ring onto the rim of the protective tube press slightly and only then tighten the screw
3. used in combination with one of the accumet pH meters Any other combination might cause loss of performance and irreversible damage to both probe and meter The fact that accuFet probes are ruggedly build to last even in harsh conditions does not mean that the probe needs no maintenance at all Read the operating tips well to ensure lasting satisfaction Also do note the following 1 Tris buffers and samples containing proteins should be read quickly and the probe should be rinsed thoroughly with deionized water between samples When testing is complete clean with water and a laboratory detergent and rinse with deionized or distilled water 2 Avoid prolonged immersion in samples containing Tris or proteins 3 Samples must be compatible with polycarbonate ABS plastic and epoxy Do not use in hydrofluoric acide DMF n n dimethylformamide MEK methylethylketone or related solvents 4 Avoid prolonged immersion in samples expected to have pH values at the ends of the specified pH range or temperature range This may shorten the probe s lifetime 5 When unavoidable rinse with ample water in between samples Rinse with neutralizing agents and distilled water when the measurement is completed and prior to storage 6 Do not use the probe outside the specified temperature range as this might result in probe failure or irreversible damage to the probe Note Lance Tip probe s temperature range is up to 60 C 7 Samples
4. C for minimal 5 minutes Then start cleaning the probe with the toothbrush After this the probe must be placed in a KCI solution with a temperature of 20 C for 1 2 hour This cold KCl dip will regenerate the reference system and the diaphragm 68X068047 rev 0 05 2004 5 8 accuFet XL Solid State Instruction Manual 8 SPECIFICATIONS Sensor Semi conductor Ion Sensitive Field Effect Transistor ISFET sensor with patented ESD protection circuit Operating temperature 5 C to 105 C For Lance Tip 5 to 60 C pH range pH 0 to 14 Reference compartment Saturated KCl gel non refillable Dimensions Probe length 200 mm Probe diameter 10 mm Cable length 1 5 meter Connector DIN Wetted materials Barrel and tip PEI poly ether imide Packaging material Epoxy resin Reference liquid Gelled KCl Diaphragm Porous PTFE Chemical resistance If doubts concerning applicability arise please contact your local dealer 9 STORAGE You can store the probes dry or for some days in KCI solution If stored in a KCl solution the value of the pH measurement can be influenced by the KCI To prevent this and to have a good measurement we advise you to clean the probe after the storage for some minutes at least 5 minutes in deionized water of about 25 C 68X068047 rev 0 05 2004 6 8 accuFet XL Solid State Instruction Manual 10 WARRANTY This accuFet probe is produced packed and shipped with the utmos
5. INSTRUCTIONS accuFet XL Solid State pH Probe Series User Manual 1 INTRODUCTION Congratulations You have just bought a accuFet XL Solid State probe one of the finest pieces of pH measuring equipment available in the world today With proper care and following the given instructions you are likely to enjoy the benefits of your accuFet XL Solid State pH system for a long time The accuFet XL Solid State series are a series of probes for use in general purposes but also in demanding applications where elevated sample temperatures up to 105 C Lance Tip only up to 60 C or contact with more aggressive chemicals are to be expected Within the accuFet XL Solid State series there are three different probe tip types available Cup Tip for general purpose use and for very small sample volumes Cone Tip for application in highly viscous materials jellies pastes slurries pulps water based polymer emulsions etc Flat Tip for direct surface measurement in paper industry water based printing inks paints coatings etc Lance Tip for application in semi solids fruit meat fish soil etc All accuFet XL Solid State pH probes contain an Ion Sensitive Field Effect Transistor ISFET sensor a silver silver chloride potassium chloride reference system and a thermistor for Automatic Temperature Compensation ATC Each probe also has an identity of its own a fingerprint so to speak enabling yo
6. cumet fishersci com MODEL NUMBER SERIAL NUMBER PURCHASE DATE a A 68X068047 rev 0 05 2004 accuFet XL Solid State Instruction Manual 8 8
7. nce of the sensor Tip 5 Buffer handling pH 7 00 buffers phosphate based and pH 4 00 buffers biphtalate based are less susceptible to carbon dioxide contamination than pH 10 00 buffers borax or carbonate based When slope errors occur it usually indicates a failing probe or a contaminated buffer If you witness this while using pH 10 00 buffer try calibrating with pH 7 00 and pH 4 00 buffer If a good slope is achieved try a new bottle or different lot pH 10 00 buffer Fresh buffers are available at Fisher Scientific ask your local dealer Tip 6 Cone Tip and Lance Tip are designed for use in semi solids or solutions When using with semi solids insert probe to desired depth then rotate left and right several times and tilt to ensure sample contact Tip 7 To avoid scratches on the chip and or epoxy it is advised only to use the toothbrush after cleaning procedure in the soapy water or after rinsing the probe in water Most scratches cause when there are hard pieces in the sample and on the ISFET Together with the toothbrush these hard pieces can cause scratches on the surface of the chip and damage the epoxy around the chip which can give bad readings 6 GENERAL 1 Reference gel may be observed as a viscous material on the tip of the probe Some gel seepage from a new probe is normal and will not affect longevity or performance Clean as above 2 Samples MUST BE aqueous liquids or semi solids Rinse with deionized o
8. r distilled water between samples 3 To ensure mixing stir samples or buffers for 5 seconds with the probe or a magnetic stirrer stop stirring and record results when stable 68X068047 rev 0 05 2004 4 8 accuFet XL Solid State Instruction Manual 4 5 6 7 7 Proteins fats and oils may be removed by scrubbing in a solution of Terg A Zyme Alconox Company Neutrad or Neutracon Decon Laboratories pepsin solution or similar product Rinse thoroughly with deionized or distilled water Spare probes should be rotated with those in active use every 30 60 days to minimize reference junction dehydration and reduce initial start up time Buffers pH 7 00 phosphate and pH 4 01 biphthalate buffers are less susceptible to CO2 contamination than pH 10 01 buffer borax or carbonate When slope error occurs it usually indicates a failing probe or contaminated buffer If seen when using pH 10 01 buffer try calibrating with pH 7 00 and pH 4 01 buffer If a good slope is achieved try a new bottle or different lot of pH 10 00 buffer When testing in direct sunlight or on a bright reflective surface use brown opaque or shielded sample containers TROUBLE SHOOTING If any of the following events occur low slope drift instability of the reading slow calibration probe will not calibrate pH value doesn t change as expected when changing samples Put the probe in soapy water with a constant temperature of 60
9. t care If the former notwithstanding defects do arise be advised that this accuFet probe is warranted to be free from defects in material and craftsmanship for the period of 6 months Fisher Scientific will repair or replace at own s option any defective part free of charge if this product fails within 6 months from the date of purchase provided that the failure is due to defective material or lack of craftsmanship and has occurred under normal conditions of usage to be judged by Fisher Scientific Fisher Scientific disclaims any liability to customers to users of its products or to any other person or persons for any special or consequential damage that might arise out of or that might in any way be connected with the use of this probe The warranty described in this paragraph shall be in lieu of any other warranty expressed or implied including but not limited to any implied warranty or merchantability or fitness for a particular purpose The buyer s sole and exclusive remedy is for repair or replacement of defective parts as provided therein All information contained in this manual is current at the time of publication Our commitment to product improvement requires that we reserve the right to change equipment procedures and specifications at any time 68X068047 rev 0 05 2004 7 8 accuFet XL Solid State Instruction Manual Electrochemical Questions Call our Technical Specialists at 1 888 358 4706 Email ac
10. ur accumet meter to identify your new probe s fingerprint through calibration 2 CLEANING TIPS 2 1 Soapy water method for cleaning the probe To have most pleasure of your probe good cleaning is a must If the probe is not properly cleaned it can happen that the probe does not work due to a polluted diaphragm or ISFET The fluid in which you are measuring can cause this Is the probe not cleaned regularly the diaphragm will block the internal electrode In such 68X068047 rev 0 05 2004 1 8 accuFet XL Solid State Instruction Manual case there is no electrical contact between the electrode and the ISFET chip and the probe will not work properly To prevent this you must clean the probe regularly The frequency is depending of the sample you are measuring in As reference it is mostly also visible the diaphragm is no longer white 2 2 Cleaning Put the probe in soapy water with a constant temperature of 60 C for minimal 5 minutes Then start cleaning the probe with the toothbrush After this the probe must be placed in a KCl solution with a temperature of 20 C for Y hour This cold KCl dip will regenerate the reference system and the diaphragm 3 SAFETY AND CARE The pH sensitive element in a accuFet XL Solid State probe is an ISFET semi conductor This sensing element needs to be driven by an electronic circuit that is built into the accumet pH meters This probe can only perform up to its specification when
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