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1. una 5 O lt C n lt C o lt Dn M y ECTFE ETFE yes PTFE yes FEP PFA yes KM yes EPDM yes NR no S yes C on C on on O O una 5 5 O Laboratory vessels have to before the sterilization in the autoclave be exactly cleaned and rinsed with the distilled water It is always necessary to remove closures from containers 1 The frequent steam sterilization is reducing mechanical durability PC test tubes are able to become useless 2 Except PCV hose which are resistant to the steam sterilization in the temperature 121 C Abbreviations of names of characterized plastics PS Polystyrene ECTFE Ethylene chlorotrifluoroethylene SAN Styrene acrylonitrile ETFE Ethylene tetrafluoroethylene PMMA Polymethyl methacrylate PTFE Polytetrafluoroethylene PC Polycarbon FEP Tetrafluoroethylene perfluoropropylene PVC Polyvinyl chloride PFA Tetrafluoroethylene perfluoroalkylvinylether POM Acetal polyoxymethylenel FKM Fluorcarbon rubber PE LD Low density polyethylene EPDM Ethylene propylene diene PE HD High density polyethylene NR Natural rubber PP Polypropylene SI Silicon rubber PMP Polymethylpentene We would like to add that for centrifuging for instance infectious materials it is necessary to use A hermetically closed buckets in order to protect their migration into the centrifuge Rotors buckets and round carriers can be sterilized in autoclave with temperature 121
2. 124 C and pressure 215 kPa during 15 min Disinfectants and cleaning agents generally used in medical care should be used in this centrifuge e g Aerodesina 2000 Lysoformin 3000 Melseptol Melsept SF Sanepidex Cutasept F AN spilled inside or outside of the centrifuge During above mentioned works one must wear safety gloves User is responsible for proper disinfections of the centrifuge if some dangerous material was 17 9 Emergency conditions service 9 1 Fault finding Majority of faults could be cancelled by switching the centrifuge OFF and then ON In the case of short duration power failure the centrifuge terminates cycle To centrifuging after power failure one should pressing the START key Please find below the most frequent faults and their repair methods Above was checked and still there is not display Call service active 2 Centrifuge does not start START key pushing does not generate reaction or single tone only P message is displayed diode of cover is shining Close cover The lock has to be locked with typical sound He has to the sign of the dot appear on the display If the diode is not switching off one shall call service diode of Start key is shining Switch power supply OFF ON If fault still persists then call service The digit of display parameters is blinking Push the Stop key which has being recorded A nmn AN program If fault still persists then call service E symb
3. It is intended for separation of mixtures suspensions and systemic fluids into constituents of different densities under influence of the centrifugal force This centrifuge is not biotight and therefore during centrifugation preparations requiring biotightness one has to use closed and sealed containers and rotors In the centrifuge it is prohibited to centrifuge caustic inflammable and explosive preparations 2 Technical data Manufacturer Type Mains LI N PE V Hz 10 Maximum power consumption Maximum speed Maximum capacity Maximum acceleration Time range Interference level Noise level Physical data Depth Width Height Weight Environmental conditions Ambient temperature Relative humidity at ambient temperature Installation category Degree of pollution Protection zone 2 1 Accessories MPW MED INSTRUMENTS SPOLDZIELNIA PRACY 46 Boremlowska Street Warsaw Poland MPW 56 100 230 V 50 60 Hz 70 W 6000 rpm 120 ml 3340 x g 00 15 99 59 min sec resolution 1 sec PN EN 55011 56 dB 270 mm 220 mm 180 mm 4 kg PN EN 61010 1 p 1 4 1 2 40 C lt 80 90 II PN EN 61010 1 2 PN EN 61010 1 300 mm 2 1 1 Basic accessories being enclosed to every centrifuge Cat No 17168 17099L 17162 17860 17866 17867 20056 ENG Type of accessories Complete clamp Spanner for the rotor Spanner for emergency opening of the cover Fuses WTA T 3 15 A 250 V Power cord 230
4. 4 1 RCF RELATIVE CENTRIPETAL FORCE N ON tA tA NJN NNN VO O VO o o 11 11 11 11 11 11 11 1 1 12 12 15 15 15 15 7 4 2 NOMOGRAM OF RELATIONSHIP ROTATIONAL SPEED CENTRIFUGING RADIUS RCF DRAWING NO 3 16 7 4 3 MAXIMUM LOAD 16 8 CLEANING DISINFECTION MAINTENANCE 16 8 1 CLEANING OF THE CENTRIFUGE 16 8 2 CLEANING OF THE ACCESSORIES 16 8 3 GLASS TUBE CRACKING 16 8 4 STERILIZATION AND DISINFECTIONS OF THE ROTATING CHAMBER AND ACCESSORIES 16 9 EMERGENCY CONDITIONS SERVICE 18 9 1 FAULT FINDING 18 9 2 WORK SAFETY INSPECTION 18 9 3 INSPECTION PROCEDURES CARRIED OUT BY THE OPERATOR 19 10 REPAIR CONDITIONS 19 11 MANUFACTURER S DATA 19 12 DISTRIBUTOR INFORMATION 19 13 TABLE OF CHEMICAL RESISTANCE TO THE INTERACTION OF VARIOUS CATEGORIES OF REAGENTS OF PLASTICS 21 Warning sings and hazard icons WARNING Warning of potential injury or health risk DANGER Risk of electric shock with potential for severe injury or death as a consequence DANGER Biohazard with potential for risk to health or death as a consequence DANGER Risk of explosion with potential for severe injury or death as a consequence gt gt P e 1 Application The MPW 56 is the laboratory centrifuge for in vitro diagnostic IVD Its construction ensures easy operation safe work and wide range of applications in laboratories engaged in routine medical analyses biochemical research works etc
5. 8 6 2 mm 14134 Round carrier for 0 2 ml test tubes b 7 8 6 2 mm TEST TUBES Catalog no Specification 15048 Polypropylene test tube 15 ml in bucket 13080 15050 Polypropylene test tube 15 ml Falcon with cap 6 17 21x120 mm for bucket 13080 15053 Polypropylene test tube 10 ml with cap b 16 19x100 mm 15054 Polypropylene test tube 6 ml with cap 6 11 7 13 5x92 mm 15101 Microhematocrite capillary tubes heparinized 1 3x50mm 15118 Glass tube 10 ml p 16x100 mm for bucket 13080 15119 Glass tube 7 ml 9 12x100 mm for round carrier 14082 in bucket 13080 15120 Glass tube 5 ml 9 12x75 mm for round carrier 14082 in bucket 13081 15121 Polypropylene test tube 10 ml with cap b 17 19x70 mm for bucket 13081 15127 Polypropylene PCR test tube 8x0 2 ml 6x21 mm with integrated caps 15123 Polypropylene test tube 2 2 ml with cap b 10 8x43 mm 15124 Polypropylene test tube 0 4 ml with cap 5 7x46 mm for round carrier 14126 15125 Polypropylene test tube 0 2 ml PCR 6 6x21 mm 15127 Polypropylene test tube 0 5 ml with cap 6 7 8x30 mm 15128 Polypropylene test tube 1 5 ml with cap 6 10 8x39 mm 15130 Polypropylene PCR test tube 8x0 2 ml 6x21 mm with separate caps 15131 Polypropylene PCR test tube 4x0 2 ml 0 6x21 mm 15419 Polypropylene test tube 5 ml p 12x75 mm for round carrier 14082 and bucket 13081 OTHER ACCESSORIES Catalog no Specification 16098 Capillary tube stoppers 16135 Hematocrite reader flat 16150 He
6. is recommended to equalize vessels loads as much as possible in order to ensure minimal vibrations during operation 10 For replacement of the rotor one shall remove test tubes and buckets release clamping by several turns of the bolt and then using both hands grab the rotor at opposite sides taking it away from drive shaft by pulling it up 6 2 Construction and safety measures The centrifuge has rigid self supporting structure Housing was made of ABS type plastic Cover is fixed on steel axles of hinges and from the front is locked with electromagnetic lock blocking possible opening during centrifugation Bowl forming the rotation chamber is made of plastic 6 3 Drive Drive constitutes induction motor of low noise level 6 4 Data input and output Data setting and read out system forms hermetically closed keyboard with distinctly accessible operation points Easily readable displays signaling individual performed operations facilitate to operator programming and recording of parameters and condition of the centrifuge 6 5 Controls The microprocessor setup of the control applied to the centrifuge is ensuring broad chances to give and of the realization of work parameters it is gt selection of rotational speed within 100 6000 rpm at 100 rpm interval gt centrifugation time within 0 60 minutes at 15 sec interval 6 6 Safety devices Apart from the above described passive devices and safety measures there exist as we
7. Serial number cene Production year MED INSTRUMENTS MPW MED INSTRUMENTS SP DZIELNIA PRACY PL 04 347 Warsaw Poland 46 Boremlowska Street Phone 48 22 610 81 07 Service Fax 48 22 610 55 36 01 06 2009 USER MANUAL Catalogue number 20056 Eng LABORATORY CENTRIFUGE MICROCENTRIFUGE MPW 56 Read this instruction before using the equipment CE io Contents 1 APPLICATION 2 TECHNICAL DATA 2 1 ACCESSORIES 2 1 1 BASIC ACCESSORIES BEING ENCLOSED TO EVERY CENTRIFUGE 2 1 2 OPTIONAL ACCESSORIES 2 2 EXPLOITATION MATERIALS 3 INSTALLATION 3 1 UNPACKING OF THE CENTRIFUGE 3 2 LOCATION 3 3 CONNECTION TO MAINS 3 4 FUSES 4 DESCRIPTION OF THE CENTRIFUGE 4 1 GENERAL DESCRIPTION 4 2 SERVICE ELEMENTS 5 SAFE WORKING CONDITIONS 5 1 SERVICING PERSONNEL 5 2 GUARANTEE AND OPERATIONAL USE PERIOD 5 3 SAFEKEEPING PERIOD 5 4 HINTS ON CENTRIFUGING 5 5 HAZARDS AND PRECAUTIONS 6 OPERATION OF THE CENTRIFUGE 6 1 MOUNTING OF THE ROTOR AND ACCESSORIES 6 2 CONSTRUCTION AND SAFETY MEASURES 6 3 DRIVE 6 4 DATA INPUT AND OUTPUT 6 5 CONTROLS 6 6 SAFETY DEVICES 6 6 1 COVER LOCK 6 6 2 REST STATE INSPECTION 7 DESCRIPTION OF THE CENTRIFUGE OPERATING ELEMENTS 7 1 CONTROL PANEL 7 2 SWITCHING THE CENTRIFUGE ON 7 3 SWITCHING THE CENTRIFUGE OFF 7 4 MATHEMATICAL RELATIONS 7
8. V Power cord 115 V optionally Operating Instruction pes 1 pes 1 pes 1 pes 2 pes 1 pes 1 pes 1 2 1 2 Optional accessories Depending on customer s needs the MPW 56 centrifuge can be provided with below specified accessories ANGLE ROTORS Cat No Type of rotor Angle Rotor capacity Max RCE ma cmm rpm em cm 11140 Angle rotor 30 6 x 15 10 ml 6000 3340 8 3 3 0 11141 Angle rotor 30 8 x 15 ml 6000 3340 8 3 3 0 11201 Angle rotor HSL 45 2x8 2x4 x0 2 ml 6000 2253 5 6 3 8 11202 Angle rotor HSL 45 12x2 2 1 5 ml 6000 2616 6 5 3 0 11203 Angle rotor HSL 45 18x0 5 ml 6000 2375 5 0 3 8 11204 Angle rotor HSL 45 24x2 2 1 5 ml 6000 2616 6 5 29 12205 Hematocrit rotor 24 capillary tubes 6000 2495 6 2 1 2 1 3x50 mm 19 ul Displayed RCF is being converting for the radius of the rotor 11140 or 11141 RCF parameter for the other rotors is being presented above HSL hermetically sealed lid BUCKETS Catalog no Application 13080 Bucket 17 7x87 mm with rubber pad for15 10 ml test tubes 6 17x120 mm 13081 Bucket 17 7x65 mm with rubber pad for 10 6 5 ml test tubes p 17x85 mm ROUND CARRIERS Catalog no Application 14082 Round carrier 13 3 for 7 6 5 ml test tubes 013 3x100 mm 14083 Round carrier 13 3 for 7 6 5 ml test tubes 013 3x100 mm 14084 Round carrier for 0 5 ml test tubes 6 10 8 8 0 mm 14126 Round carrier for 0 4 ml test tubes 9 10 8 5 7 mm for rotor 11202 14133 Round carrier for 0 2 ml test tubes 6 10
9. W MED INSTRUMENTS Poland PL 04 347 Warsaw 46 Boremlowska Street Tel 48 22 6105667 Export and sales department 48 22 6108107 Service department Fax 48 22 610 55 36 E mail mpw mpw pl www mpw pl 12 Distributor information YOUR DISTRIBUTOR 19 Centrifuging radius cm r p m R C F x g 90 multiple of 20000 gravitational acceleration 45 15000 100000 14000 40 13000 Formula used for calculation of this 12000 oneal 50000 11000 40000 m R C F 11 18 r n 1000 0000 30000 9000 where 30 20000 8000 R C F multiple of 7000 F gravitational speed 15000 27 r centrifuging radius cm 6000 26 n rotational speed r p m 10000 B 25 g gravitational acceleration 8000 5000 24 6000 23 5000 4500 22 4000 C 4000 Al 3000 3500 20 2400 19 2000 3000 1500 h 1200 2500 M 1000 E ai 2000 1 600 500 400 4 1500 13 300 1400 1300 42 200 1200 Example of making use 150 1100 11 of the nomogram 120 1000 100 900 i 800 60 A 14 4 cm 9 B 4600 r p m 50 700 C 3400 x g 40 30 25 8 20 500 15 RCF 400 d n 21000 11 18 r 8 6 t RCF 6 300 T un lt 9 5 n 4 11 18 1000 3 200 Drawing No 3 Nomogram 20 13 Table of chemical resistance to the interaction of various categories of reagents of plastics Groups of the substance in temp 20 C Aldehydes Cyclic alcohols Esters Ether Ketones Strong or concentrated acids Weak or diluted acids Ox
10. am of relationship rotational speed centrifuging radius RCF Drawing No 3 7 4 3 Maximum load In order to avoid overloading of the rotor one shall observe maximum load which is recorded on every rotor Maximum permissible load is reached when all test tubes are filled with the fluid with 1 2 g cm density If density of the centrifuged liquid is higher than 1 2 g cm then test tubes could be filled only partially or one shall limit operation speed of the centrifuge that is being calculated from the formula 17 a G n perm n max y specific gravity E Nmax maximum rotational speed rpm y cm 8 Cleaning disinfection maintenance CAUTION It is necessary to use protective gloves during following work 8 1 Cleaning of the centrifuge For cleaning shall be used water with soap or other water soluble mild detergent One should to avoid corrosion inducing substances and aggressive substances It is prohibited to use alkaline solutions inflammable solvents or agents containing abrasive particles Using wiping cloth remove from the rotor chamber condensate or residues of the products It is recommended to keep the cover opened when the centrifuge does not work in order to expel the moisture 8 2 Cleaning of the accessories In order to ensure safety operation one shall in regular way carry out periodical maintenance of the accessories Manufactured rotors buckets and round carriers have to withstand steady high st
11. be used to starting the centrifugation program with the parameters displayed on display blinking DIODE on the START key element No 3 signalling rotary motion of rotor The centrifuge can be activated if the cover is closed showing up of sign of the dot on the display element No 10 the DIODE is not shining on the COVER key element No 5 STOP key element No 1 serves for aborting the actually running operation interrupting centrifuge program in any program phase and braking the rotor finishing of rotor braking process on the display will shine sign S Stop also signalling this state by sound 12 COVER key element No 4 serves for open the centrifuge cover Open or incorrectly closing the cover is signalling by shining DIODE key is active only if the rotor is not centrifuging The cover can be opened only if rotor is stopped on the display will be displayed sign S and the centrifuge will signal possibility of opening cover by five short sounds ATTENTION It is not possible to open of the centrifuge at moment when the sign signalling of possibility to change the parameter value is active even in spite of the stopped rotor The field of functional keys serves for change the program as well for setting it s individual parameters such as speed RCF time After pressing the key the sign equal appears on the display It means that the value of given parameter can be changed b
12. e operated by laboratory personnel after getting acquainted with Operating Instruction Operating Instruction shall be held all the time near the centrifuge Operating Instruction must be kept close always at hand 5 2 Guarantee and operational use period Guarantee period for the MPW 56 centrifuge amounts to minimum 24 months Principles are specified in guarantee certificate The service life of the centrifuge specified by the manufacturer amounts to 10 years After termination of guarantee period it is necessary carry out yearly technical inspection of the centrifuge by service authorized by manufacturer The manufacturer reserves the right to make modifications at produced goods 5 3 Safekeeping period Maximum period of storage of not used centrifuge amounts to 1 year After this period one should carry out technical inspection of the centrifuge by service authorized by manufacturer 5 4 Hints on centrifuging AN AN AN A AN Set the centrifuge in horizontal position on rigid base Ensure safe positioning location Ensure free space around the centrifuge amounting to at least 30 cm left free Ensure sufficient ventilation Fix the rotor on the motor axis firmly Avoid unbalance Load opposite buckets with the same accessories Centrifugation of the test tubes of different dimensions The possibility to centrifuge test tubes of different dimensions is existing however it is absolutely necessary in
13. idizing acids or oxidizing substances cyclic hydrocarbons Ahs Haloid hydrocarbons Alkalis PS SAN PMMA PC O O O O O O O very good chemical resistance Permanent action of the substance isn t causing damage through 30 days The material is able to be resistant through years o chemical resistance of good to limited PCV POM PE LD PE HD PP O ETFE FEP PFA O O O O O O O O O O O O O O O O O O O O O PMP ECTFE PTFE FKM EPDM NR Continuous action of the substance is causing insignificant damage through the period of 7 30 days partly reversible e g puffing up softening reduced mechanical durability discoloring limited chemical resistance The material isn t able to have the continuous contact with the substance The immediate occurrence of damage is possible e g the loss of mechanical durability the deformation discoloring bursting dissolving Abbreviations of names of characterized plastics PS Polystyrene SAN Styrene acrylonitrile PMMA Polymethyl methacrylate PC Polycarbon PVC Polyvinyl chloride POM Acetal polyoxymethylenel PE LD Low density polyethylene PE HD High density polyethylene PP Polypropylene PMP Polymethylpentene ECTFE ETFE PTFE FEP PFA FKM EPDM NR SI Ethylene chlorotrifluoroethylene Ethylene
14. itch the centrifuge on if it is not installed properly or rotor is not fitted correctly The centrifuge must not be operated in places where explosion hazard appears as it is not explosion proof make It is prohibited to subject to centrifuge materials which subjected to action of air could generate inflammable or explosive mixtures It is prohibited to subject to centrifuge toxic or infectious materials with damaged leak proof seals of the rotor or test tube Proper disinfections procedures have to be carried out when dangerous substances contaminated the centrifuge or its accessories It isn t allowed to open the cover manually in emergency procedure when rotor is still turning It isn t allowed to exceed load limit set by the manufacturer Rotors are intended for fluids of average homogeneous density equal to 1 2 g cm or smaller when centrifugation is carried out at maximum speed When fluids of higher density shall be used then it is necessary to limit speed see point 7 4 3 Maximum load It isn t allowed to use the rotors and round carriers with signs of corrosion or other mechanical defects It isn t allowed to centrifuge substances of high corrosion aggressiveness which could cause material impairment and lower mechanical properties of rotor and round carriers It isn t allowed to use rotors and accessories that one not admitted by the manufacturer Let to use commercial glass and plastic test tubes which are destined t
15. ll active devices and elements as follows 6 6 1 Cover lock The centrifuge can be started only with properly closed cover In turn the cover can be opened only after stopping the rotor by COVER key In the case of emergency opening of the cover during operation the centrifuge will be immediately switched off and the rotor will brake till complete stop With opened cover COVER diode is shinning drive is completely disconnected from the power that makes impossible to start the centrifuge 6 6 2 Rest state inspection Opening of the centrifuge s cover is possible only with the rotor in the state of rest This state is being checked by the microprocessor which recognizes and signals the rest state prior to opening the cover 11 7 Description of the centrifuge operating elements Power switching ON OFF is carried out with master switch situated on right side of the centrifuge All settings on the centrifuge are done by means of the control panel Panel comprises control keys LCD display and signalling diodes 7 1 Control panel For controlling centrifuge operation serves control panel placed on front casing wall 10 5 9 4 D N A r PWO Centrifuge MFU F6 3 F 2 6 M xe 00000000 0M 1 Drawing No 2 Control panel 1 STOP key 5 COVER diode 9 Increasing key 2 START key 6 TIME key 10 Display 3 START diode 7 Decreasing key 4 COVER key 8 SPEED RCF key START key element No 2 on the Drawing No 2 can
16. matocrite reader round while stock last 2 2 Exploitation materials For operating in centrifuge one should use only original company s buckets comprised in the A specification of accessories as well as test tubes for centrifuges of proper diameter length and strength Utilization of test tubes of other makes shall be agreed upon with manufacturer of the centrifuge For cleaning and disinfecting one should to use agents generally used in the health service such as e g Aerodesina 2000 Lysoformin 3000 Melseptol Melsept SF Sanepidex Cutasept F 3 Installation 3 1 Unpacking of the centrifuge Open the package Take out the cardboard box containing the accessories Take out the centrifuge from the package Keep the package and packing materials at hand for possible transport at a later date 3 2 Location The centrifuge shall not be located near the radiators and shall not be subjected to direct sunlight A The table for the centrifuge shall be stable and shall have flat leveled table top It is necessary to ensure the safety zone around the centrifuge of the minimum 30 cm from every direction Normal operating conditions ambient temperature is from 15 C to 35 C Passed parameters of the spinner are referring to above named temperatures At the change of the place from cold for warm condensation of water will occur inside the centrifuge It is important then that sufficient time shall be provided for drying the centrifuge prior t
17. ns next pressing cause switching between tens minutes digit minutes digit tens secunds digit and secunds digit choosen digit will start blinking and it is possible to set it with buttons No action for 3 seconds cause exit from setting mode with saving setpoint LCD display element No 10 The centrifuge has readable display LCD on which are showing information being referred to the actual condition of the system The information about the centrifuge type program version and internet address are displaying at once after switching supply on for three seconds following the information about program has been lately made the time and speed settings will be displayed 14 sign S means the rotor is stopped the underline sign rotor is working time the sign means that the cover is closed and the 0 means the cover is open after pressing the SPEED RCF key twice element No 8 on the Drawing No 2 the display shows the acceleration value corresponding to the programmed speed The sign means that the given parameter can be modified During operation of the centrifuge it s impossible to change the program parameters but only interrupt its further realization with pressing the STOP key element No 1 7 2 Switching the centrifuge on After acquainting with operation elements and preparing the centrifuge to operation one shall set the speed and time next close the cover and pre
18. nt because of safety reasons are not damaged This remark is specifically important for 1 Motor suspension 2 Motor axis concentricity 3 Centrifuge accessories and especially structural changes corrosion preliminary cracks abrasion of metal parts 4 Screw joints 5 Inspection of the rotor assembly 6 Control for guarantee yearly technical inspection of the centrifuge Only the manufacturer specified holders included on the equipment list as well as centrifuge capillaries which diameter length and durability are suitable should be used for spinning in this centrifuge The use of equipment made by other manufacturers should be consulted with the manufacturer of the centrifuge Disinfectants and cleaning agents generally used in medical care should be used in this centrifuge e g Aerodesina 2000 Lysoformin 3000 Melseptol Melsept SF Sanepidex Cutasept F 10 Repair conditions Manufacturer grants to the Buyer a guarantee on conditions specified in the Guarantee Certificate Buyer forfeits the right to guarantee repair when using the device inconsistently with the Operating Instruction provisions when damage resulted from the User fault Repairs should be carried out in authorized service workshops granted with the MPW Certificate The centrifuge shall be sent to repair after decontaminating disinfections Information about authorized service workshops could be obtained from the Manufacturer 11 Manufacturer s data MP
19. o centrifuging in this laboratory centrifuge It is distinct warning against using poor quality elements Cracking of vessels could result in dangerous unbalance It isn t allowed to lift or shift the centrifuge during operation and rest on it It isn t allowed to stay in the safety zone within 30 cm distance around the centrifuge neither leave within this zone some things e g glass vessels It isn t allowed to put any objects on the centrifuge 10 6 Operation of the centrifuge 6 1 Mounting of the rotor and accessories 1 Connect the centrifuge to the mains plug in socket at back of the centrifuge Switch on the centrifuge by master switch at the right of the centrifuge 3 Open the cover of the centrifuge by pressing the COVER key Prior to putting the rotor in one has to check if rotating chamber is free of impurities e g such as dust glass splinters residues of fluids that must be taken away 4 One shall fit the rotor on the motor shaft driving it home on the cone 5 Screw in the bolt for fixing the rotor clockwise and screw it tightly home with the supplied spanner for the rotor 6 One should use only buckets intended for selected types of the rotor see p 2 1 Accessories 7 Fill test tubes beyond the centrifuge 8 Test tubes buckets have to be filled properly in order to avoid overflows 9 CAUTION Centrifuge will tolerate small weight differences occurring during loading of rotors However it
20. o repeat starting the centrifuge minimum 4 hours 3 3 Connection to mains Supply voltage given on the rating plate has to be consistent with local supply voltage A MPW MED INSTRUMENTS laboratory centrifuges are in I safety class devices and they are provided with the three core cable with the plug resistant to dynamic loadings Mains socket shall be provided with the safety pin It is recommended to install emergency cut out that shall be installed far from the centrifuge near the emergency exit or beyond the room Supply voltage 100 230 V 50 60 Hz Before switching on check whether the centrifuge is connected to power supply correctly Check centrifuge before usage whether she is installed correctly 3 4 Fuses The centrifuge has standard protection with the WTA T 3 15 A 250 V fuse Fuse is situated in the plug in socket unit at back wall of the centrifuge 4 Description of the centrifuge 4 1 General description New generation of MPW MED INSTRUMENTS laboratory centrifuges is provided with the modern microprocessor control systems very durable and quiet asynchronous brushless motors and accessories consistent with the modern requirements of user 4 2 Service elements lI gt 0 INA 1 Power switch 2 Control panel 3 Emergency cover open 4 Cover 5 Inspection glass Drawing No 1 General view 5 Safe working conditions 5 1 Servicing personnel The MPW 56 laboratory centrifuge can b
21. ol displayed after stopping Drive overload Wait for 15 minutes and switch again after Eo tiet te eee pegado eee o With the attempt opening cover is audible buzzing of One should lift up till the yellow Cover is switching on Failed spring of cover lifting or bended the lock striker One should bend the striker or call service diode Cover is not shining and the centrifuge not Lock is failed Call service swirling Emergency cover release In the case of e g mains failure it is possible to open the cover hand Place the key 17162 into the hole on the right side of the casing and push in The cover will be opened A The cover can be unlock and opened only when the rotor is in the rest state 9 2 Work safety inspection It is necessary for safety reason to inspection the centrifuge carried out by the authorized service at least once a year after the period of warranty The reason for more frequent inspections could be corrosion inducing environment Examinations should end with issuing Report of validation the check on the technical state of the laboratory centrifuge Is being recommended to establish Technical passport or Log of the apparatus whom every repairs and reviews are being registered in Both these documents should to deposit in the place of use the centrifuge 18 9 3 Inspection procedures carried out by the operator Operator has to pay special attention to the fact that the centrifuge parts importa
22. resses originated from the field of gravitation Chemical reactions as well as corrosion combination of variable pressure and chemical reactions can cause corrosion or destruction of metals Hard to observe surface cracks increase gradually and weaken material without visible symptoms In case of observation of surface damage crevice or other change as well the corrosion given part rotor bucket etc shall be immediately replaced In order to prevent corrosion one has to clean regularly the rotor with the fastening bolt buckets and round carriers Cleaning of the accessories shall be carried out outside of the centrifuge once every week or still better after each use Then those parts shall be dried using soft fabric or in the chamber drier at ca 50 C Especially prone to the corrosion are parts made of aluminium For cleaning them one should use very neutral agent of pH value from 6 to 8 It is forbidden to use alkaline agent of pH above 8 In this way substantially is increased useful service life and diminished susceptibility to corrosion Accurate maintenance increases as well service life and protects against premature rotor failures Corrosion and damages resulting from insufficient maintenance could not be object of claims lodged against the manufacturer 8 3 Glass tube cracking In the case of glass tube cracking all debris shall be accurately removed and then rubber inserts shall be exactly cleaned or possibly replaced Otherwise one ha
23. s to take into account following possibilities Glass particles left in the rubber cushion pad will cause once more glass cracking Glass particles left in the rotor chamber cause plastic abrasion because of strong air circulation This dust will not only contaminate the centrifuge chamber rotor buckets carriers and centrifuged material but will cause as well damages of surfaces of the accessories rotors and the rotation chamber For complete removal of glass particles and metal dust from the rotor chamber it is recommended to place on the bowl strip of vaseline from the top down to bottom Then rotor shall operate for several minutes at speed 200 2500 rpm Glass and particles will collect on lubricated area and could be easily removed with the piece of cloth together with the grease This operation can be repeated in case of need 8 4 Sterilization and disinfections of the rotating chamber and accessories One can use all standard disinfectants The centrifuges and accessories are constructed from various materials and one should to take into account possible variety of materials During sterilization by means of steam one should to consider temperature resistance of individual materials 16 STERILIZATION Sterilization Radiation B y Gas Chemical compounds temp 121 C 25 kGy ethylene oxide formalin ethanol time 20 min P SAN PMMA PC PVC OM PE LD PE HD 5 o OJ n o n 5 5 5 Ojo LY o C on
24. ss the START key The centrifuge will start and realize the programmed parameters 7 3 Switching the centrifuge off The centrifuge is automatically switching off when the program is realized It is possible to finish earlier the realization of given program by pressing the STOP key After ending the centrifugation process one should remember to switch off the centrifuge using the main switch element No 1 Drawing 1 being located on the right side of centrifuge 7 4 Mathematical relations 7 4 1 RCF relative centripetal force RCF acceleration this 1s the acceleration generated by the rotor rotary motion acting upon tested product and it can be calculated according to the formula RCF 11 18 x r x n 1000 RCF xg r cm n rpm Depending on the distance of particles of the tested product from the axis of rotation one can find from above formula minimum RCF average RCF or maximum RCEF On the basis of preset RCF value and given radius of the bottom in the bucket one can calculate from the formula rotational speed to be set in the program of centrifuging Selection of the time of sedimentation and the RCF value shall be carried out experimentally for a given product Once every 100 rpm electronic circuit automatically calculates and displays RCF value In order to program required RCF value one shall use nomogram Drawing No 3 or change rotational speed matching displayed value to required acceleration value 15 7 4 2 Nomogr
25. such cases that opposite buckets and round carriers have to be the same It is necessary to insert test tubes symmetrically Fill test tubes beyond the centrifuge Please pay special attention to the quality and proper thickness of the glass test tubes walls Those shall be test tubes for centrifuges of proper strength 5000 x g Fill in the test tubes with the same weight in order to protect the centrifuge against unbalance Use only accessories kept in good condition Protect equipment against corrosion using accurate preventive maintenance Infectious materials could be processed in closed test tubes only It is prohibited to centrifuge explosive and inflammable materials It is prohibited to centrifuge substances prone to reacting in result of supplying high energy during centrifugation 5 5 Hazards and precautions gt Prior to starting the trial of switching the centrifuge on one shall read exactly all sections of this instruction in order to ensure smooth run of operation avoiding damages of this device or its accessories Centrifuge shall not be operated by unqualified personnel gt gt eie e Pe e gt Centrifuge must not be transported with the rotor mounted on the shaft One must use original rotors test tubes and spare parts only In case of faulty operation of the centrifuge one shall ask of assistance of service of MPW MED INSTRUMENTS or its authorized representatives It is prohibited to sw
26. tect our employees please fill out the declaration of decontamination completely before sending centrifuge to the manufacturer return 5 Device STYPE qj qj dgissstisst tds aaa Bd RO NE PIECE ET eM E LU PAR DDEMME NT serial No 6 Description of decontamination see user Manual 7 Decontamination carried out by name 8 Date and signature ooneacnicii i at e Env vet Hei n aoa Dv Eon Veg Cb ku Ru Ead
27. tetrafluoroethylene Polytetrafluoroethylene Tetrafluoroethylene perfluoropropylene Tetrafluoroethylene perfluoroalkylvinylether Fluorcarbon rubber Ethylene propylene diene Natural rubber Silicon rubber ZA SI MED INSTRUMENTS Y Product Laboratory centrifuge Model MPW 56 Jd yn onthe basis of Non classified to list A or B and no the Directive 98 79 EC self testing Product complies with the requirements PN EN 61010 1 2011 PN EN 61010 2 020 2008 Directive 2004 108 WE EMC standard PN EN ISO 15223 1 2012 MPW MED INSTRUMENTS CZ ONEK ZAR PREZESZ pn m SPOLDZIELNIA PRACY iind her Warsaw 46 Boremlowska Street Quality policy in line with ISO 9001 2008 Certifying authority MPW MED INSTRUMENTS SPOLDZIELNIAPRACY w Warszawie DECLARATION OF CONFORMITY Warsaw 13 11 2014 DECLARATION OF DECONTAMINATION In order to protect our employees please fill out the declaration of decontamination completely before sending centrifuge to the manufacturer repair 1 Device SAVDO qj 585 ldeatbrreiseive dota eaiotaaite teuad mets tiewetsaa ped uuo TO E A NA LEANNE RR 2 Description of decontamination see user Manual 3 Decontamination carried out by SAAME qj J kRkKIJk lqll Qo nnnm 4 Date and signature sitossdiivtindtied aste rime ectorene esi crt aefost OO DECLARATION OF DECONTAMINATION In order to pro
28. y key arrow down or arrow up The possibility of changing the value of parameter is signalling by sign and is active for a three seconds It is time when one should accede to set the demanded value After three seconds from the setting of the demanded value of given parameter this value will be saved in the program or after selection the given program will be set as active Decreasing key arrow down element No 7 serves for decreasing the values of parameters Increasing key arrow up element No 9 serves for increasing the values of parameters SPEED RCF key element No 8 serves for selecting the possibility of changing the centrifuging speed SPEED next pressing of the key switching over the programming mode from speed to RCF values Rotational speed can be setting from 100 rpm to 6000 rpm with 100 rpm interval RCF with 100xg interval Pressing button will occur entry to speed RCF setting mode switching between rpm and RCF setting follow with the next pressing the key Setting the value is set in step at 100 using the buttons There are no actions for 3 seconds to exit the setting mode with saving setpoint 13 TIME key element No 6 serves for programming the centrifuging time in minutes from 15 sec to 99 min 59 sec every 1 second single pressing cause entry to time setting mode the tens minuts digit will start blinking it is possible to setting it with butto
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