ESP-101 - Eicom USA
Contents
1. e To facilitate loading the pusher block 1 can be released from the leadscrew and manually moved along the guide rods to accommodate the syringe e Press the bronze release button 2 on the side of the black pusher block 1 to release theblock from the leadscrew e Raise the spring loaded retaining clamp knob 3 and rotate away from the syringe barrel e Place the syringe barrel in the V of the syringe holder making sure that the flange of the syringe barrel is pressed against the side of the syringe holder e Rotate and release the syringe clamp to hold the syringe in place e Press in release button 2 and move the pusher block back along the guide rods to makecontact with the syringe plunger GLASS SYRINGES With some glass syringes the corners of the flange of the syringe barrel are rounded and can cause a tendency for the syringe barrel to ride up out of the syringe holder To give a more secure flatter surface to clamp against an O ring or metal collar can be placed over the barrel and pressed against the flange ESP 120 PUSH PULL PUMP Travel Direction Withdraw Syringe Clamp Release Button Withdraw Bracket Syringe Rest e To facilitate loading the pusher block can be released from the leadscrew by pressing the bronze release button 1 and can be manually moved along the guide rods to accommodate the syringes e First place the withdraw syringe in the withdraw syringe holder using the spring loadedretaining c2. warranty gives you specific legal rights You may also have other rights which vary from state to state Note This pump is not registered with the FDA and is not for clinical use on patients Eicom USA Eicom Europe 7098 Miratech Drive Suite 100 Ground Floor Hilton House San Diego CA 92121 Ardee Rd Rathimines United States Dublin 6 Ireland Tel 888 680 7775 Fax 858 560 8040 Tel 353 1 902 2700 Fax 353 1 443 0784 www eicom usa com www eicomeurope com US eicom usa com info eicom usa com EU eicomeurope com info eicomeurope com
3. 274 ul h 3164 l h 6359 ul h 13 2 ml h ESP 101 Synge Si ESP 120 US eicom usa com info eicom usa com EU eicomeurope com info eicomeurope com 14 Limited Warranty e Eicom USA warrants to the first consumer purchaser for a period of one year from the date of purchase that this unit when shipped in its original container will be free from defective workmanship and materials and agree that it will at its option either repair or replace the defective unit e This warranty does not extend to misuse neglect or abuse normal wear and tear accident modification or unauthorised repair e Ficom USA will not be liable or in any way responsible for any incidental or consequential economic or property damage Some States do not allow the exclusion of incidental or consequential damages so the above exclusion may not apply to you e There are no implied warranties of merchantability or fitness for a particular use or of any other nature Some states do not allow this limitation on implied warranty so the above limitation may not apply to you e f a defect arises within the warranty period contact Eicom USA e The customer is responsible for shipping charges and must first obtain a Return Material Authorization number RMA before the unit will be accepted If a replacement unit is issued it is covered only for the remainder of the original warranty period dating from the purchase of the original device e This
4. GEEEpE EE 10 12 Japanese Table of Syringe Diameters assisas i eae a E Ee N 11 13 Standard Minimum and Maximum Flow Rates oo ccc 12 14 5 Limited Warrants tesa anna 13 US eicom usa com info eicom usa com EU eicomeurope com info eicomeurope com 1 Specifications 100 Series Including ESP 100 101 and 120 Syringe Size 10 microliter to 60 milliliter Electrical Rating US model 115V 0 1A CE model 230V 0 06A 6x20 mm 250V slow blow 0 1A Voltage Operating Range US model 100 120V 50 60Hz CE model 200 240V 50 60Hz Drive Mechanism microprocessor controlled stepper gearmotor 1 2 microcrostepping driving a leadscrew through a belt and pulley drive mechanism Pusher Advance per Half Step 0 529 micron or 2 083x10 5 inch ESP 100 0 088 micron ESP 101 Linear Travel ESP 100 ESP 120 minimum 0 00635 cm hr maximum 76 18 cm hr ESP 101 minimum 0 00106 cm hr maximum 12 2 cm hr Minimum Stepping Rate one 1 2 step in thirty seconds may vary depending on syringe size Maximum Stepping Rate 400 1 2 steps second Speed Range 12000 1 Flowrate Range 0 1pI hr 10 ul syringe to 506ml hr 60 ml syringe 20 Ib min 9 Km Dimensions 9x6x5 inch 23x15 25x13 cm 4 5 Ib 2 Kam Atmospheric Specifications 4 C to 40 C 40 F to 104 F 20 to 80 RH non condensing e sn SSS N A e CSA US eicom usa com info eicom usa com EU eicomeurope com info eicomeurope com Cooling Conditions No special considerations Mech
5. User s Manual EiCom Microdialysis syringe Nanoliter Infusion Pump FA Eicom Model ESP 101 WEEE RoHS Compliance Statement EU Directives WEEE and RoHS To Our Valued Customers We are committed to being a good corporate citizen As part of that commitment we strive to maintain an environmentally conscious manufacturing operation The European Union EU has enacted two Directives the first on product recycling Waste Electrical and Electronic Equipment WEEE and the second limiting the use of certain substances Restriction on the use of Hazardous Substances RoHS Over time these Directives will be implemented in the national laws of each EU Member State Once the final national regulations have been put into place recycling will be offered for our products which are within the scope of the WEEE Directive Products falling under the scope of the WEEE Directive available for sale after August 13 2005 will be identified with a wheelie bin symbol Two Categories of products covered by the WEEE Directive are currently exempt from the RoHS Directive Category 8 medical devices with the exception of implanted or infected products and Category 9 monitoring and control instruments Most of our products fall into either Category 8 or 9 and are currently exempt from Do Not Dispose Product with Municipal Waste the RoHS Directive We will continue to monitor the application of the RoHS e Special Collection Disposal Requi
6. anical Stability No special considerations 2 General Safety Summary Please read the following safety precautions to ensure proper use of your syringe pump To avoid potential hazards and product damage use this product only as instructed in this manual If the equipment is used in a manner not specified by the manufacturer the protection provided by the equipment may be impaired To Prevent Hazard or Injury e Use Proper Power Supply The pump is supplied with an approved power supply and line cord e Use Proper Line Cord Use only the line cord shipped with the product and make sure line cord is certified for country of use e Ground the Product This product is grounded through the grounding conductor of the power cord To avoid electric shock use only approved line cord with the product and insure it is connected to earth ground e Make Proper Connections Make sure all connections are made properly and securely e Orient Equipment Properly Do not position the equipment such that it is difficult to reach the disconnecting device e Observe all Terminal Ratings Review the operating manual to learn the ratings on all connections e Avoid Exposed Circuitry Do not touch any electronic circuitry inside of the product e Do Not Operate with Suspected Failures If damage is suspected on or to the product do not operate the product Contact qualified service personnel to perform inspection e Avoid Pinch Hazard A pinch hazard may
7. d to clean the pump A mild detergent solution may e be used to clean the keypad US eicom usa com info eicom usa com EU eicomeurope com info eicomeurope com 11 Standard Table of Syringe Diameters 1 Air Tite All Plastic 2 Becton Dickinson Interim WW design Plastipak 3 Becton Dickson Glass all types 4 Hamilton 1000 Series Gastight 5 Popper amp Sons Inc Perfektum glass 6 Ranfac fs EFF 1455 20 1986 23 20 27 0 7 Scientific Glass Engineering SGE 8 Sherwood Monojet Plastic e no eo 6 568 9 Terumo B00 EN El O w foe eo o jos i 10 Unimetrics Series 9000 US eicom usa com info eicom usa com EU eicomeurope com info eicomeurope com 12 Japanese Table of Syringe Diameters Available in Japanese Models 1 Air Tite All Plastic 2 Becton Dickinson Interim WW design Plastipak EN dee 5 ma 14 48 20 fow 21 59 3 Becton Dickson Glass all types 4 Hamilton 1000 Series Gastight US eicom usa com info eicom usa com EU eicomeurope com info eicomeurope com 5 JMC Air Tite pls EN d O EN ET HE w fo 8 Natsume 13 Standard Minimum and Maximum Flow Rates ESP 100 Syringe Size 10 ul 25 ul 50 ul 100 ul 250 ul 500 ul 1 ml Minimum 0 1yu 0 1yu 0 2 u 1 04 2 04 3 04 0 01 ml h h h h h h h Maximum 126 ul h 318 ul h 625 ul h 1
8. exist between the Pusher Block and End Blocks Avoid placing fingers between these points while the pump is running e Observe all Warning Labels on Product Read all labels on product to ensure proper usage CAUTION Protective Ground Refer to Manual Terminal CAUTION This pump is not registered with the FDA and is not for clinical CAUTION use on human or veterinary patients It is intended for research FOR RESEARCH USE ONLY use only NOT FOR CLINICAL USE ON PATIENTS US eicom usa com info eicom usa com EU eicomeurope com info eicomeurope com 3 Features The Model ESP100 series are simple accurate cost effective syringe pumps designed to hold glass or plastic syringes of any make from 10 microliter to 60 milliliter for the ESP 100 10 ul to 15 ml for the ESP 120 and 10 ul to 10 ml for the ESP 101 The models ESP 100 and ESP 101 are infusion only pumps The flow rates of the ESP 101 are six times slower than that of the ESP 100 and the units of flow rate are suitably adjusted The ESP 120 is a push pull simultaneous infusion withdrawal pump Setup and pump operation for all of the 100 series pumps are similar and extremely simple A menu displayed on an alphanumeric LCD prompts the operator to make the necessary selections using the keypad for choice of features and numerical entries The internal diameter of the syringe is used by the control program to calibrate the pump and deliver the volume and flowrate selected F
9. ges are entered the pump immediately changes to the new settings e While operating press select to return to the main menu e Select Volume to display the set dispense volume If no change is required press select e For a volume change use the arrow keys and enter with the select key e The display moves to RATE permitting a change if required Press select if no change is required or use the arrow keys to change the setting The select key immediately changes to the new flowrate and the volume display continues to increment uninterrupted to the newdispense setting NOTE If the VOLUME is changed to a setting smaller than the volume already accumulated then the pump stops when the new smaller dispense volume is selected CHANGE OR REVIEW RATE SETTING WHILE RUNNING e Press select to change the volume accumulator display to the main menu e Select RATE e Make rate changes if required and press select The pump immediately changes to the new flow rate and displays the continuing volume accumulation uninterrupted by the change in flow rate FAST FORWARD Press the run and the keys simultaneously to actuate the fast forward mode The pump travels at its maximum rate while both keys are pressed simultaneously 6 Loading The Syringe ESP 100 AND ESP 101 3 Syringe Clamp Knob Syringe Plunger 1 Pusher Block Syringe Holder 2 Release Button US eicom usa com info eicom usa com EU eicomeurope com info eicomeurope com
10. hods of diameter entry e Internal Library Select TABLE from the main line menu The table of syringes is organized by manufacturer then by glass or plastic and then by size Use the arrow keys to scroll through the table and the select key to enter the correct setting First scroll through the manufacturers and select the manufacturer of thesyringe used The menu will next display either syringe type or syringe size Again use the arrow keys to move through the table until the correct size is displayed Press the select key when the correct syringe size is displayed This automatically enters the internal syringe diameter See Table of Syringe Diameters on page 12 for the syringe library e Direct Entry From the main menu select DIA The display will read xx xx mm Use the arrow keys to display the measured internal diameter of the syringe in millimeters and enter with the select key Changing the diameter clears the Volume and Rate settings and the display will now prompt for the new Volume setting VOLUME ENTRY The volume setting mode is selected from the main menu or is displayed automatically after changing the diameter Display reads xx xx ul Units displayed in ul or ml depend on syringe diameter entered e Use the arrow keys to enter the dispense volume required e When the correct dispense volume is displayed save this number with the select key NOTE If no target dispense volume is required enter volume 0 0 The pump will
11. lamp to retain the syringe barrel in the V of the syringe holder e Make sure the barrel flange is held firmly by the withdraw clamp and that the adjusting screws on the withdraw clamp are firmly tightened e While keeping the button 1 pressed firmly in slide the block along the guide rods so that the syringe plunger flange can be retained by the clamp on the pusher block Tighten the locking screws on the adjusted syringe clamp e Loosen the locking screw on the Adjustable syringe rest and position the rest so that the plunger of the loaded syringe is pressed firmly against the pusher block and the syringe barrel flange is pressed up to the syringe rest e The barrel of the loaded infusion syringe should be clamped in the V of the syringe holder with the spring loaded clamp e Tighten the screw on the syringe rest to lock the rest in place on the guide rod US eicom usa com info eicom usa com EU eicomeurope com info eicomeurope com 7 Manual Stall Setting and Microliter Syringes e A movable collar located on the rear guide rod can be set to restrict travel of the pusher block The block moves until stalling against the collar Stalling does no permanent damage but may result in increased wear on the drive mechanism and should not be used routinelybut only as a fail safe device e Microliter syringes with fine wire plungers can be damaged if the plunger is forced into theend of the syringe barrel the collar can be adjus
12. ng or highlighted feature of the main menu display e Enter numerical values RUN STOP e Turns the motor on and off e Acts as a pause during a dispense DISPLAY After the pump is programmed an arrow on the right side of the display indicates the direction of operation This arrow pulses when the pump is running US eicom usa com info eicom usa com EU eicomeurope com info eicomeurope com 5 Operating Instructions POWER SWITCH The power switch is located at the right rear corner of the pump When the power is turned on the LCD will display the Volume or Rate setting Press the select key to return to the main menu MAIN MENU Pressing select repeatedly will always return the display to the main menu The main menu consists of five variables three of which are displayed at all times The center variable pulses to indicate that this option can be reviewed or changed The menu acts as a continuous loop and the arrow keys are used to move around the loop The variable to be changed can be selected with the select key direct entry syr dia mm flowrate ul or ml h dispense vol ul or ml SYRINGE DIAMETER ENTRY Syringe diameter must be entered for automatic internal calibration Once entered the diameter is entered it is retained in non volatile memory and need not be entered again unless the syringe being used is changed When a syringe diameter is changed the Volume and Rate settings are set to zero There are two met
13. or simplicity the syringe diameter is also used to set automatically the volume and flowrate units The syringe diameter can be entered directly or the syringe can be identified from a table of syringes held in memory When the syringe is selected from the table the diameter is entered automatically Two dispense modes are available e Disspense volume mode in which the pump keeps track of the volume dispensed and automatically stops the pump when a set target volume is reached e Run mode where the pump runs at the set flowrate until manually stopped In the event of a power interruption during operation the pump can be programmed to either resume operation or remain stopped when power is returned For convenience all pump settings are stored in non volatile memory to minimize the number of setting changes required 4 Keypad Functions This key has two functions e Moves the display to the left e Causes the numerical entry to decrease To change the displayed number by a single unit press and release the key quickly If the key is pressed longer the number changes with increasing speed This key has two functions e Moves the display to the right e Causes the numerical entry to increase To change the displayed number by a single unit press and release the key quickly If the key is pressed longer the number changes with increasing speed SELECT This key has three functions e Return to main menu when pressed repeatedly e Selects the pulsi
14. pense the volume accumulator is not cleared the dispense is paused Restarting the pump with a second press of the run stop key continues the dispense to the target volume POWER FAILURE MODE If there is a temporary power interruption when no dispense volume is selected the Power Up run stop option allows the pump to either resume the dispense when the power returns or remain stopped but display a message warning of the power failure e Move the main menu to the right and select PWR UP e The display will read Power Up Run or STOP Use the arrow keys to change display and select the required option RUN e After power is returned the pump resumes normal operation The display flashes PowerFailed to acknowledge a power interruption Press Select to display the Rate STOP e Pump does not run when power returns The LCD displays the rate setting If a dispense volume is entered the power interruption stops the pump clears the volume accumulator and returns the volume to the setpoint Only if the power failure setting is run will the power failure warning be displayed US eicom usa com info eicom usa com EU eicomeurope com info eicomeurope com CHANGE OR REVIEW VOLUME SETTING WHILE RUNNING While the pump is running it is possible to look at the volume and flowrate settings without interrupting the operation of the pump If required the volume and flowrate settings can be changed while the pump continues to run When the chan
15. red Directive to its products and will comply with any changes as they apply Contents 1 SPECIMEN iins ean ann ana ande 2 2 General Safety SUMMALY cccccccccesecscsecscecscsecsssecsesscsecscsecsssecsssessssessessssesassesassessesesseasseaseeeass 3 3 e S nada sevcntsheann aint anspiindansa vs Sivnanahitnngn a0 oendbiilannns ius cantGuangann ernennen naher eh 4 A Keypad FUNCIONS een handen anna EEOAE ONRET 4 5 Operating Instructions POWO NO Ea TERCHTERT ER SEERTERRR EIER STERRREVRLE TERCHELERRELTRLTRRCHERTERGRTERRTRLETERCRETE ARTE 5 Mannes 5 Synno Diameter ENNY erretreta ee erene eee EEEE ecededecncadesbandcedeseieaacesoiecadhdoseiecacasaueens 5 Volume ENUY ea ee 5 FLOCIE EI eet ee ae ran a en 6 Starting TS PUT esha ads en E EEE EE 6 Power Failure Mode enter RERA ARE A EARE ERARA 6 Rate and Volume Changes While RUNNING s icscvscerervscaesiwvertecaweeatiesbavtenteubeveenwvestinebenvenewe 7 ASU FONAN asare tessnstdubetsstdstluatisnvsdasitiassghesiedteasirestas seth dthustniasdietensdurtnatecsdtaienanantiig 7 6 Loadingith SYNJ En cvehccdeceedacstccbevacnbeddimbonticbenatacbcdibdcciastenihcdatdantecddcadedbcdecbddiec cubes cbedines 7 8 7 Manual Stall Settings and Microliter Syringes eueeesneseeeeeneeennesennennnnenennennnnennnnnnennnnennnnenennennnennnn 9 8 NV Hami FAUNE sinan E a E O A OA 9 E E nea A A A A A E E 9 10 MAMENANG aranan e E E De ee 9 Tis Standard Tabie ot coy ON DIENEN S sirsenis piepie eie pasetea pieiet EREE
16. run at the set flowrate until stopped manually or a stall occurs After setting a volume the menu prompts for the flowrate setting e When the pump runs the actual dispensed volume will be displayed and willincrement until the set volume is reached at which time the pump will stop automatically US eicom usa com info eicom usa com EU eicomeurope com info eicomeurope com FLOWRATE ENTRY RATE can be selected from the main menu or will be displayed automatically after the volume setting The display reads Rate X X pl h or X X ml h Rate units are ul h or ml h microliter or milliliter hour and are selected automatically according to the syringe diameter e Use the arrow keys to display the flowrate required e Enter the rate with the select key e f the rate entered is out of range a message is displayed Max Rate XX ml h To continue enter a lower rate or select a larger NOTE e If a dispense volume was set then the display will now change to Volume 0 0 that is in automatic dispense mode When the pump runs the actual dispensed volume will be displayed and will increment until the set volume is reached at which time the pump will stop automatically e f no dispense volume is set the LCD displays the set flowrate andn when running the directional indicator arrow will pulse START THE PUMP e Press the run stop key to start the pump A second press will stop the pump e f the pump is stopped during a dis
17. ted to prevent this occurrence 8 NV Ram Failure e f the settings in the non volatile memory become corrupted the display will read NV Ram Failure and the pump will not operate e To recover from this condition the pump must be powered down and then turned on again e The pump will be re initialized to the default settings and can now be programmed as normal e A second method of clearing the NV Ram Failure is to press select and then enter a changed rate setting 9 Fuses The fuses are located in the power entry module on the rear panel Fuse Holder e The linecord must be first removed to gain access to the fuse holder e Fuses 5x20mm 250V slow blow 0 1A Voltage Selector CE version only If it is necessary to change the input voltage selection disconnect the line cord from the entry module on the rear panel Use a flat bladed screwdriver to open the Fuse Holder access door Remove the Fuse Holder flip over and reinstall Close the access door The new input voltage selection should be visible through the door window Install a proper line cord certified for the country of use 10 Maintenance e Maintenance is required only for the moving mechanical parts which should be kept clean and lubricated Occasionally a small amount of light machine oil should be applied to the guide rods and a small amount of grease or oil to the leadscrew e Solvents of any type should never be use
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