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The Genevac EZ-2 Series Personal Evaporator User Manual

1. Run finished Three long beeps when system comes to rest Repeats every 5 minutes until any user interaction Button pressed Run select switch moved Lid opened Key Acknowledge Short beep Illegal key press Short double beep Other Troubleshooting SYMPTOM Mains switch not illuminated CAUSE Supply failure ACTION Check the system is switched on and supply is available Fuse failure Refer to maintenance section LCD not operating As above LCD not illuminated System in shutdown mode Press start button Run does not appear to start Chamber preheating cooling Pre heat and pre cool are indicated on the display The run will start when the system is ready Error has occurred Check display for information messages and take appropriate action See previous table Rotor will not start to spin Drive belt failure Refer to maintenance section Drive motor fuse failure Consult Genevac for advice No vacuum Pump fuse failure Consult Genevac for advice Pump failure Refer to maintenance section Vacuum fails to pull down below 900mbar at start of a run Lid not fully closed Stop run and check for obstructions Lid not sealing Stop run and check for obstructions fit and condition of lid seal Replace if required Vacuum pulls down to 500mbar but rate slows dramatically DriPure The system is running correctly Vacuum pulls b
2. 2 Safety and Maintenance Notes Ensure that safety and maintenance notes Page vi are complied with throughout this work instruction 3 Special tools and equipment Description Part number Quantity LAMP GLASS ASSEMBLY 70 1116 S 1 ALLEN KEY 4mm AB9603 1 mm mm mm mm mm mm PO ip gt 04 5359 Issue 1 6 ENSURE THAT EVAPORATOR IS ISOLATED FROM MAINS POWER SUPPLY ANY_MAINTENANCE OR REPAIR OF THIS PRODUCT NOT DETAILED IN THIS MANUAL SHALL BE CARRIED OUT BY GENEVAC PERSONNEL OR APPROVED REPRESENTATIVES OF GENEVAC USING ONLY APPROVED SPARE PARTS THIS SYMBOL MEANS THAT YOU SHOULD NOT TOUCH SURFACES IN THESE AREAS THESE SURFACES CAN BE VERY HOT AND MAY LEAD TO BURNS Traces of solvents may be present that could be harmful to your health or lead to material damage There may be a risk to sample integrity if lamp fails to operate or required vacuum is not achieved October 2008 Section 3 Chapter 5 Page 1 Genevac EZ 2 Series Personal Evaporator User Manual 4 4 1 Removal Remove Lamp as detailed in Chapter 4 A LAMP GLASS ASSEMBLY MAY BE HOT 4 2 4 3 4 4 4 5 4 6 5 1 5 2 5 3 5 4 5 5 Allow assembly to cool if system used recently Using allen key remove lamp assembly securing screw SECURING SCREW Remove lamp assembly Check lamp assembly to chamber location face for damage Clean using approved method see item 2 4 Clean
3. Chapter 3 Fuses 1 Introduction 2 Safety and Maintenance Notes 3 Special tools and equipment 4 Removal 5 Refitting 6 Testing 7 Completion Chapter4 Lamp Unit 1 Introduction 2 Safety and Maintenance Notes 3 Special tools and equipment 4 Removal 5 Refitting 6 Testing 7 Completion Chapter5 Lamp Glass Assembly 1 Introduction 2 Safety and Maintenance Notes 3 Special tools and equipment 4 Removal 5 Refitting 6 Testing 7 Completion Chapter 6 Lid Seal 1 Introduction 2 Safety and Maintenance Notes 3 Special tools and equipment 4 Removal 5 Refitting 6 Testing 7 Completion 04 5359 Issue 1 6 October 2008 Page ii Genevac EZ 2 Series Personal Evaporator User Manual Chapter7 Pump 1 Introduction Safety and Maintenance Notes Special tools and equipment Removal Refitting Testing Completion NOOR WN Chapter8 Pump Head Components 1 Introduction 2 Safety and Maintenance Notes 3 Special tools and equipment 4 Removal 5 Refitting 6 Testing 7 Completion SECTION 4 TECHNICAL SPECIFICATION Mechanical Data Vacuum system Temperature controls Condenser Data Solvent compatibility Dimensions Inert Gas supply Power Supplies Power Consumption Environment Operating Storage Emissions EC DECLARATION OF CONFORMITY SAFETY WARRANTY STATEMENT USEFUL INFORMATION 04 5359 Issue 1 6 October 2008 Page iii Genevac EZ 2 Series Personal Evaporator User Manual 4 Introd
4. Vacuum leak Carry out user vacuum test Incorrect run selected Check that the run selected is most appropriate Low control temperature set Check that correct temperature is set for the compound Contamination of lamp Clean lamp glass glass Contamination of IR Clean IR sensor lens Caution refer to sensor lens maintenance section Condenser fuse failure Refer maintenance section Incorrect sample holder for sample format Consult Genevac for advice Samples not dry when auto end of run stops run Individual sample volumes were very different or low De select auto end of run when carrying out such runs or better still top up samples to the same level with solvent Samples dry but not dry enough Various factors can cause this Consult Genevac for advice Excessive quantity of solvent present in catchpot exhaust condenser Or poor solvent recovery Incorrect run selected Check that the run selected is most appropriate Condenser jar full Ensure condenser jar is emptied after every run Vacuum leak Verify by user vacuum test and check causes above Condenser fuse failed Consult Genevac for advice Volatile solvent Some very volatile solvents will not be trapped in the internal condenser jar use a Genevac external exhaust condenser Body of system gets hot This is acceptable under normal operation Excessive vibration H
5. Borosilicate glass R404a Up to 165 C Only with option Only with option 570 mm 597 mm 645 mm 700 mm 88 kg EZ Bio 2150 500G Direct 1 5 kg 40g 0 1150 mbar 10 mbar atm Yes 10 mbar Yes Ambient to 80 C 2 5 C infra red pyrometer 99 to 99 C Ambient Timed 40 C 1 1 litre Borosilicate glass R404a Up to 120 C N A N A 570 mm 645 mm 700 mm 82 kg Section 4 Page 1 Genevac EZ 2 Series Personal Evaporator User Manual Power Supplies 120V 10 60Hz 15A USA 230V 410 50Hz 13A UK Europe 100V 10 50Hz 15A Japan 100V 10 60Hz 15A Japan Power Consumption Peak 230V 50Hz 1610VA 120V 60Hz 1800VA 100V 50 60Hz 1200VA Note The systems may momentarily take current in excess of the figures quoted above Genevac therefore recommends the use of appropriately rated type D circuit breakers on the main supply to the EZ 2 Environment The following figures apply Operating Ambient Temperature 15 C to 30 C Relative Humidity 10 60 Altitude Sea Level to 1 600m Storage Ambient Temperature 10 C to 60 C Relative Humidity 10 80 Altitude Sea Level to 12 000m Emissions Noise levels do not exceed 55dBA at one metre from the evaporator 04 5359 Issue 1 6 October 2008 Section 4 Page 2 Genevac EZ 2 Series Personal Evaporator User Manual EC Declaration of Conformity We Genevac Limited Declare that this product EZ 2 Evaporating
6. Ensure that both the Condenser Jar and the Catchpot are emptied prior to starting the run With the key selector set to NORMAL the EZ 2 can be used without an inert gas supply being available NORMAL PURGE SUPPLY MAX 1 5 bar PURGE MIN 1 0 bar LL FILTER DRAIN PURGE IN Key Switch Purge In Filter Drain With the key selector set to PURGE the purge function is available Ensure that the inert gas supply meets the pressure requirements as detailed in the Technical data section Ensure that the EZ 2 lid is closed and that the holding bar latched and the thumb screws qty 2 tightened BEFORE PURGE TURN TO LOCK AND CLAMP BAR AT BOTH ENDS 4 TO RELEASE LID UNSCREW KNOBS UNTIL BAR MOVES FREELY UNDER VACUUM BAR MAY AUTOMATICALLY RELEASE Press START on EZ 2 control panel screen will display PrG The purge period is set for 300 seconds 5 Mins If you pause a run at any time during the purge cycle the system will reset and automatically purge the system again When the run is complete or is stopped the system will automatically vent and return the chamber to atmospheric pressure during which time a blanket of inert gas is maintained in the chamber to protect the samples With_inert gas purge unit IGP key selector set to PURGE ensure that the gas supply is always available during the runs Inert purge gases The system is suitable for use with either nitrogen or argon which should be dry C
7. Lamp Unit 2 Safety and Maintenance Notes Ensure that safety and maintenance notes Page vi are complied with throughout this work instruction 3 Special tools and equipment Description Part number Quantity LAMP AC9060 1 ALLEN KEY 4mm AB9603 1 mm mm mm mm mm mm Ei He gt 04 5359 Issue 1 6 ENSURE THAT EVAPORATOR IS ISOLATED FROM MAINS POWER SUPPLY ANY MAINTENANCE OR REPAIR OF THIS PRODUCT NOT DETAILED IN THIS MANUAL SHALL BE CARRIED OUT BY GENEVAC PERSONNEL OR APPROVED REPRESENTATIVES OF GENEVAC USING ONLY APPROVED SPARE PARTS THIS SYMBOL MEANS THAT YOU SHOULD NOT TOUCH SURFACES IN THESE AREAS THESE SURFACES CAN BE VERY HOT AND MAY LEAD TO BURNS Traces of solvents may be present that could be harmful to your health or lead to material damage There may be a risk to sample integrity if lamp fails to operate or required vacuum is not achieved October 2008 Section 3 Chapter 4 Page 1 Genevac EZ 2 Series Personal Evaporator User Manual 4 Removal 41 Open lid A LAMP UNIT MAY BE HOT 4 2 Allow lamp to cool if system used recently 4 3 Disconnect mains power supply 4 4 Using allen key remove the 3 securing screws from lamp access panel located on the right hand side viewed from the front PANEL SCREWS 4 5 Remove panel PURGE DO NOT ATTEMPT TO ADJUST OR REMOVE THE SPRING LOADED BOLTS AS THE FOLLOWING WARNING IS ONLY APPLICABLE TO AN
8. There is even a special method for HPLC fractions such as Acetonitrile water mixtures QUICK REFERENCE GUIDE mn COOLING VENTS LOCATION SLOT i j ene ac Evaporation Pure and sim pe tor y evaporato ew CONDENSER JAR CATCH POT LID ___ LAMP ACCESS PANEL HAND HOLD POWER SWITCH HAND HOLD Fig 1 04 5359 Issue 1 6 October 2008 Page iv Genevac EZ 2 Series Personal Evaporator User Manual The use of the high efficiency solvent resistant Teflon diaphragm pump which is integral to the evaporator saves you space and provides quiet efficient control of the vacuum Combined with a new glass jar condenser that s easy to empty the Next Generation EZ 2 Series is our most compact and reliable sample evaporator yet AUTO STOP WHEN DRY x MAXTENp o aa so Sc 4 65 nz 75 MEDIUMBP 4 MED LOW bo BPMIXTURE HPLC FRACTION Dri Pure Fig 2 EZ 2P Control Panel Compared with a typical rotary evaporator the EZ 2 Series can process many more samples per unit time Simply select the solvent type and the maximum allowable sample temperature Smart evaporation software in the EZ 2 Series does the rest for you setting the vacuum conditions and gradient whilst controlling the heater lamp and automatically stopping the run once the samples are dry True walk away automation so simple any one can use it with confidence Genevac s patented sample protection sy
9. EZ 2 FITTED WITH INERT GAS A INDICATED BELOW SPRING LOADED BOLTS AN DO NOT TOUCH 04 5359 Issue 1 6 October 2008 Section 3 Chapter 4 Page 2 Genevac EZ 2 Series Personal Evaporator User Manual 4 6 SLIDE SIDEWAYS OUT OF CLIPS 4 7 5 5 1 5 2 5 3 5 4 Carefully withdraw lamp unit from clip assembly by sliding sideways Refitting Unpack replacement lamp unit Locate lamp unit in to clip by sliding sideways Connect lamp power lead by gently pushing on to lamp contacts Refit access panel and secure with screws A DO NOT USE SYSTEM WITHOUT PANEL BEING FITTED 5 5 6 1 6 2 6 3 6 4 6 5 6 6 7 1 Reconnect Mains power supply Testing For an EZ 2 select AQUEOUS For an EZ 2plus select Medium Boiling Point For an EZ Bio select H20 Set temperature to 60 C Press START button Check that lamp illuminates Completion Dispose of defective lamp and packaging 04 5359 Issue 1 6 October 2008 Disconnect lamp power lead from lamp unit by pulling gently on ceramic holder not leads PULL GENTLY TO DISCONNECT Section 3 Chapter 4 Page 3 Genevac EZ 2 Series Personal Evaporator User Manual Chapter 5 Lamp Glass Assembly 04 5359 Issue 1 6 October 2008 Section 3 Genevac EZ 2 Series Personal Evaporator User Manual 1 Introduction This chapter provides guidance in the removal replacement and refitting of Lamp Glass Assembly
10. Evaporator User Manual Evaporation pure and simple eva orator owe ban 1 Empty Condenser Jar 7 Close lid 2 Switch ON 8 Set Max temperature as you would on a rotary evaporator 3 Press and wait for 90 second safety countdown 9 Select solvent type see Quick Reference 4 Press a when LED is lit 10 Press gt your run will start 5 Open lid 6 Load your samples into the holders Ensure that samples are balanced and suitable for use in the EZ 2 Please refer to User Manual Page viii Safety Whilst the system is running the screen will indicate the progress of the run Please see Section 1 Chapter 3 for more details 04 5359 Issue 1 6 October 2008 Section 1 Chapter 2 Page 1 Genevac EZ 2 Series Personal Evaporator User Manual A5 Quick reference card Please see the A5 reference card located in the slot on the EZ 2 for the quick start guide Contents are as follows To start a run Empty condenser jar Turn on It is recommended that the evaporator is left on Press By Open lid Load the system with your samples balanced to within 10g Close Lid Select Solvent Group using rotary selector Set the desired Maximum Sample Temperature using rotary selector Press gt To disable the Auto Stop facility press and hold gt button for 5 seconds maximum run duration is now set Actual duration is governed by the run and sample temperature selected Run duration may now be adjus
11. Main Supply 6 3A 04 3587 1 ENSURE THAT EVAPORATOR IS ISOLATED FROM MAINS POWER SUPPLY ANY MAINTENANCE OR REPAIR OF THIS PRODUCT NOT DETAILED IN THIS MANUAL SHALL BE CARRIED OUT BY GENEVAC PERSONNEL OR APPROVED REPRESENTATIVES OF GENEVAC USING ONLY APPROVED SPARE PARTS 04 5359 Issue 1 6 October 2008 Section 3 Chapter 3 Page 1 Genevac EZ 2 Series Personal Evaporator User Manual The 2 mains supply fuse holders F6 and F7 are standard screw type located next to the mains power connector on the left hand side of the evaporator viewed from the front Label identifies Power Supply requirements and Fuse ratings 4 Removal 4 1 Ensure evaporator is isolated from mains supply 4 2 Unscrew both fuse holders 43 Remove defective fuses 5 Refitting 5 1 Fit replacement fuses to holders 5 2 Refit fuse holders to evaporator 6 Testing 61 Re apply mains power 7 Completion 7 4 Dispose of defective fuses If a fuse blows more than once please contact Genevac Service Department To ensure uninterrupted service always ensure a spare fuse is available 04 5359 Issue 1 6 October 2008 Section 3 Chapter 3 Page 2 Genevac EZ 2 Series Personal Evaporator User Manual Chapter 4 Lamp Unit 04 5359 Issue 1 6 October 2008 Section 3 Genevac EZ 2 Series Personal Evaporator User Manual 1 Introduction This chapter provides guidance in the removal replacement and refitting of
12. Methanol fractions Water Acetonitrile 500G Centrifuge at atmosphere with temperature control Available on E7 2P only For EZ Bio see Section 1 Chapter 3 Page 2 Detailed Operation for Solvent Group Guide and Run Selection 04 5359 Issue 1 6 October 2008 Section 1 Chapter 2 Page 2 Genevac EZ 2 Series Personal Evaporator User Manual ERROR WARNINGS If an error occurs during a run then one of 2 things will happen 1 The run will be terminated This means that the EZ 2 has detected a failure in something that may have an effect on Sample Integrity The physical condition of the evaporator An error code will be displayed when the rotor has stopped spinning and the pressure is at atmosphere 2 The run will continue in a reduced features mode This means that the EZ 2 has detected a failure in something that does not have any effect on Sample Integrity The physical condition of the evaporator An error code will be displayed when the rotor has stopped spinning and the pressure is at atmosphere INDICATION CAUSE OF ERROR RECTIFICATION PSH Strt NO ERROR Push button OPn Lid NO ERROR Open Lid Err 05 Lid closed but not locked Open and re close lid Check lid lock release screw Err 06 Tried to open lid before it is unlocked Reset and try again this time wait for beep before lifting lid Err Lid Lid open during run Open and re close lid Err Loc Lid not
13. Personal Evaporator User Manual 1 Introduction This chapter provides guidance in the updating of Software Software Data Key 2 Safety and Maintenance Notes Ensure that safety and maintenance notes Page vi are complied with throughout this work instruction 3 Special tools and equipment Description Part number Quantity Software Data Key Dependant upon 1 Machine and Software requirements ANY MAINTENANCE OR REPAIR OF THIS PRODUCT NOT DETAILED IN THIS MANUAL SHALL BE CARRIED OUT BY GENEVAC PERSONNEL OR APPROVED REPRESENTATIVES OF GENEVAC USING ONLY APPROVED SPARE PARTS OBSERVE PRECAUTIONS ELECTROSTATIC SENSITIVE DEVICES gt 04 5359 Issue 1 6 October 2008 Section 3 Chapter 1 Page 1 Genevac EZ 2 Series Personal Evaporator User Manual 4 4 1 4 2 4 3 5 1 5 2 5 3 6 1 7 1 8 1 Preparation Ensure that evaporator is switched OFF Remove blanking cap from connector on evaporator Plug software Data Key into connector Uploading Switch on evaporator The evaporator will automatically load software The existing code has to be deleted first The display will show ErS bLc n where n is the block number Each block could take up to 8 seconds and there are 4 blocks to erase There will be no progress indication during each block erase cycle The block number will increment as each block has been erased The software will
14. System Complies with the relevant Essential Health and Safety Requirements of the European Machinery Directive 89 392 EEC as amended by 91 368 EEC and 93 44 EEC The EMC Directive 89 336 EEC and the Low voltage Directive 73 23 EEC Conformity is demonstrated by compliance with the following specifications EN 60204 1 1998 Safety of machinery Electrical equipment of machines Pt 1 General Requirements EN 249 1992 Safety of machinery Safety distances to prevent danger zones being reached by upper limbs EN 1088 1996 Safety of machinery Interlocking devices associated with guards Principles of design and selection BS EN ISO 12100 pts 1 8 2 2003 Safety of Machinery Basic concepts general principles for design BS EN 50082 1 1998 Electromagnetic compatibility Generic immunity standard BS EN 61010 2 020 1995 Safety requirements for electrical equipment for measurement control and laboratory use Particular requirements for laboratory centrifuges 04 5359 Issue 1 6 October 2008 Safety WARNING THIS SYSTEM MUST BE EARTHED THIS EVAPORATOR IS A SAFETY CLASS 1 PRODUCT ACCORDING TO IEC CLASSIFICATION IT MUST NEVER BE USED WITH ANY _ INTERRUPTION TO THE SAFETY EARTH CONDUCTOR IT IS AN INSTALLATION CATEGORY II PRODUCT AND IS INTENDED TO OPERATE FROM A NORMAL SINGLE PHASE SUPPLY THIS EVAPORATOR HAS BEEN DESIGNED TO BE USED IN A POLLUTION DEGREE 1 ENVIRONMENT NO POLLUTION OR ONLY DRY NON COND
15. check contents against packing note 9 If not already fitted fit inlet filter 10 04 5359 Issue 1 6 October 2008 Section 1 Chapter 1 Page 3 Genevac EZ 2 Series Personal Evaporator User Manual 11 Fit Condenser Jar DURING ITEM 12 THE EXHAUST HOSE MUST BE FITTED THIS HOSE MUST THEN BE DUCTED TO A SUITABLE EXTRACTION UNIT AREA OR A FUME HOOD 12 Connect the long exhaust hose supplied and duct into fume hood This is particularly important if HCI or other acids are being used I Er To maintain a solvent vapour free environment around your EZ 2 we suggest that you do not use free space within the hood for storage of vessels containing solvents or acids Users should also ensure that tubing leading to and from the solvent condenser leads solvents and solvent vapours away from the machine in a safe manner In articular the exhaust tube must be fitted to the outlet from the condenser and lead away to the laboratory fume extraction system This will ensure that any residual vapour which passes through the condenser and pump does not cause damage to your system 13 Connect mains supply lead supplied y 04 5359 Issue 1 6 October 2008 Section 1 Chapter 1 Page 4 Genevac EZ 2 Series Personal Evaporator User Manual Chapter 2 QUICK START 04 5359 Issue 1 6 October July 2008 Section 1 Genevac EZ 2 Series Personal
16. to the above would result in the costs of repairs being charged Unit supplied in reusable packaging This is to be retained for reuse by client in the unlikely event that system needs to be returned for repair If package not retained a charge will be made for replacement packaging and shipping costs incurred 04 5359 Issue 1 6 October 2008 Section 4 Page 4 Genevac EZ 2 Series Personal Evaporator User Manual Genevac Limited The Sovereign Centre Farthing Road Ipswich IP1 5AP United Kingdom Sales and Service Hotlines Service Hotline 44 0 1473 243000 Sales Hotline 44 0 1473 240000 Fax 44 0 1473 461176 Email salesinfo genevac co uk Web site http www genevac com Genevac Inc SP Industries 815 State Route 208 Gardiner NY 12525 United States of America Sales and Service Hotline 1 845 267 2212 Fax 1 845 267 2212 Email salesinfo genevacusa com 04 5359 Issue 1 6 October 2008 Useful information If you need to contact Genevac for assistance use either the telephone or fax Hotlines given It will always help Genevac Service if you have the model and serial numbers at hand for the components of your system If you need to contact Genevac Sales for information on Service Contracts or products use the telephone or fax Hotlines given Alternatively Email or visit our web site Section 4 Page 5
17. 5 HPLC Fraction 1889 885 709 506 443 443 Medium BP 833 695 416 260 209 174 Medium low BP 1041 1041 1041 695 521 416 Spare 1440 1440 1440 1440 1440 1440 Low Solvent Volumes Where the volume of solvent is low and or the number of Samples Tubes is small the total run time may need to be adjusted to ensure that the samples are dry See Setting Run Time for details Genevac and the ATEX Directive Please note that it remains the responsibility of the user to consider any solvents being evaporated within the context of the ATEX directive The presence of solvents on the list above indicates only that they will not damage the system If further information is required please contact your Sales Representative or visit http www genevac com 04 5359 Issue 1 6 October 2008 Section 1 Chapter 3 Page 1 Genevac EZ 2 Series Personal Evaporator User Manual Solvent Group Guide EZ 2 amp E7 2 Complete Solvent Abbreviation Group HOAc EtOOH ethanoic acid LOW BP MEDIUM BP Acetonitrile LOW BP MeOOH LOW BP MEDIUM BP Hept hp LOW BP Pride py LOWBPMEDIUMBP Examples HPLC FRACTIONS WATER METHANOL or WATER ACETRONITRILE LOW BP MIXTURES DCM METHANOL MED LOW BP MIXTURES DMF DCM Note A risk assessment must be carried out before use especially when used in conjunction with other organic solvents because H O will give off O2 when under vacuum creating an oxygen rich atmosphere So
18. ANY MAINTENANCE OR REPAIR OF THIS PRODUCT NOT DETAILED IN THIS MANUAL SHALL BE CARRIED OUT BY GENEVAC PERSONNEL OR APPROVED REPRESENTATIVES OF GENEVAC USING ONLY APPROVED SPARE PARTS Er Traces of solvents may be present that could be harmful to your health or lead to material damage There may be a risk to sample integrity if required vacuum is not achieved 04 5359 Issue 1 6 October 2008 Section 3 Chapter 8 Page 1 Genevac EZ 2 Series Personal Evaporator User Manual 4 Removal 4 PUMP HEAD ASSEMBLY Fig 1 4 1 Remove pump as detailed in Chapter 7 and place on suitable work surface 4 2 Remove pump from tray by removing attachment screws 4 3 Note and mark position of PTFE tubes 4 4 Disconnect the 2 PTFE tubes from the connectors on the pump head 4 5 Using Allen Key remove the 4 retaining screws from the pump head 4 6 Remove pressure plate 4 7 Carefully remove the connecting head 4 8 Carefully remove valves qty 2 and O ring seals qty 2 Fig 3 4 9 Carefully remove pump head 04 5359 Issue 1 6 October 2008 Section 3 Chapter 8 Page 2 Genevac EZ 2 Series Personal Evaporator User Manual 4 10 Using Pulley Spanner carefully locate in Strain Washer and rotate anticlockwise Fig 4 4 11 Remove Strain Washer Diaphragm and Pressure Disc assembly PRESSURE PLATE CONNECTING HEAD O RING PUMP PRESSURE DIAPHRAGM STRAIN SEALS amp HEAD DISC WA
19. ART outside dead diia as Chapter3_ DETAILED OPERATION siii ai ii 04 5359 Issue 2 July 2006 Section 1 Genevac EZ 2 Series Personal Evaporator User Manual Chapter 1 UNPACKING AND SETTING UP 04 5359 Issue 1 6 October 2008 Section 1 Chapter 1 Page 1 Genevac EZ 2 Series Personal Evaporator User Manual Flight Case 1 Undo 4 quick release fasteners 2 Carefully remove lid i Refer to photographs on Pages 2 to 4 inclusive of this Chapter for aditional guidance 3 Position base and EZ 2 near to operating bench location 4 With assistance 3 people total lift EZ 2 from base and position where required on bench 5 Ensure that there is 50mm clearance from edge of bench and other equipment 6 Refit lid and store flight case 7 Check contents of accessories container 8 Fit inlet filter 9 Fit condenser jar 10 Fit Catchpot DURING ITEM 11 THE EXHAUST HOSE MUST BE FITTED THIS HOSE MUST THEN BE DUCTED TO A SUITABLE EXTRACTION UNIT AREA OR A FUME HOOD 11 Connect the long exhaust hose supplied and duct into fume hood This is particularly important if HCI or other acids are being used do not use free space within the hood for storage of vessels containing solvents or acids Users should also ensure that tubing leading to and from the solvent condenser leads solvents and solvent vapours away from the machine in a safe manner In particular the exhaust tube must be fitted to the outlet from
20. Genele f Evaporation Pure and simpl MP evaporator evaporator an General Information This manual has been produced to assist in the daily running and routine maintenance of the Genevac Evaporator Amendment Control Form Only the affected pages and the Front Page will display the Revision Number all others will stay at initial issue Revision Issue and Reason for Change Date Issued Number 2 Introduction of Inert Gas Purge IGP Additional advice regarding the use 25 August 2006 of Hydrogen peroxide 3 Minor updates 13 February 2007 4 WARNING regarding lamp replacement on EZ 2 with Inert Gas Purge 26 April 2007 5 Information regarding ice formation and thawing in Condenser Jar 16 January 2008 1 6 Update U S address 24 October 2008 These instructions are subject to change without notice No part of these instructions may be reproduced in any form or be processed duplicated or distributed by electronic or optical means without the written permission of Genevac Limited All rights reserved Genevac Limited These operating instructions should be read before you use the Genevac EZ 2 Series Personal Evaporator Keep them near the system for easy reference Your attention is drawn in particular to Page vi Paragraph 2 2 Safety 04 5359 Issue 1 6 October 2008 CONTENTS Amendment Control Form 1 INTRODUCTION 2 SAFETY AND MAINTENANCE NOTES 2 1
21. PORATOR IS ISOLATED FROM MAINS POWER SUPPLY ANY MAINTENANCE OR REPAIR OF THIS PRODUCT NOT DETAILED IN THIS MANUAL SHALL BE CARRIED OUT BY GENEVAC PERSONNEL OR APPROVED REPRESENTATIVES OF GENEVAC USING ONLY APPROVED SPARE PARTS 04 5359 Issue 1 6 October 2008 Section 3 Chapter 2 Page 1 Genevac EZ 2 Series Personal Evaporator User Manual 4 Removal 4 1 Remove ALL samples sample holders and swings from evaporator 4 2 Remove condenser jar catchpot and drip plate 4 3 Ensure that the lid is closed and locked Failure to do so could result in damage to the system when placing evaporator on its back side 4 4 Disconnect mains power supply i During item 4 5 place protective padding between evaporator and bench surface 4 5 With assistance move evaporator to suitable bench and carefully lower evaporator onto its back 4 6 Remove drive belt access panel securing screws 4 7 Remove drive belt access panel 4 8 Visually check condition of belt ensure free from splits or tears 4 9 If drive belt is intact and condition is acceptable check tension using special tool 04 3658 4 10 Rotate the pulleys so that the tightest part of the belt is at the measurement point This is done by feel only Mark this point on the belt using marker pen or chalk 4 11 Place a rubber band nominally 160mm long 1mm thick 6mm wide max over plain part of pulleys Ensure band is sitting flat and not on either the groove or the edge 4 12 Sli
22. SHER VALVES 4 12 Separate the three items 04 5359 Issue 1 6 October 2008 Section 3 Chapter 8 Page 3 Genevac EZ 2 Series Personal Evaporator User Manual 5 Refitting 5 1 Remove replacement components from service kit packaging 5 2 Ensure that all items are correct Fig 5 3 Do not use any assembly oils or fluids when reassembling the pump Clean distilled water may be used to locate seals and valves during assembly 5 3 Assemble Strain Washer Diaghragm and Pressure Disc Fig 5 54 Carefully locate onto pump connecting rod Fig 6 5 5 Using Pulley Spanner carefully locate in Strain Washer and rotate clockwise Fig 4 56 Carefully refit pump head 5 7 Carefully fit valves qty 2 into pump head Fig 3 5 8 Carefully fit O ring seals qty 2 into pump connecting head Fig 3 5 9 Carefully fit pump connecting head 5 10 Refit pressure plate 5 11 Refit securing screws qty 4 5 12 Reconnect the 2 PTFE tubes to the pump head connections 5 13 Refit pump tray and secure with attachment screws 5 14 Refit pump as detailed in Chapter 7 6 Testing 6 1 Test as detailed in Chapter 7 7 Completion 7 1 Safely discard old seals and valves 04 5359 Issue 1 6 October 2008 Section 3 Chapter 8 Page 4 Genevac EZ 2 Series Personal Evaporator User Manual SECTION 4 TECHNICAL SPECIFICATION 04 5359 Issue 1 6 October 2008 Genevac EZ 2 Series Personal Evaporator User Ma
23. Symbols 2 2 Safety 2 3 Collection jar fitting and removing 2 4 Cleaning 2 5 Genevac Evaporators and Combustible Solvents 2 6 Safe loading of rotor 2 7 Lid operation 2 8 Limitations of use SECTION 1 GENERAL Chapter 1 UNPACKING AND SETTING UP Flight Case Consumer Packaging Chapter2 QUICK START A5 Quick reference card Chapter3 DETAILED OPERATION SOLVENT LIMITATIONS Evaporation of Acid Chlorides Evaporation of Di ethyl ether Evaporation of High Boiling Point Solvents Solvent Group Guide EZ 2 amp EZ 2 Quick Guide Maximum Run Times Low Solvent Volumes Solvent Group Guide EZ 2 amp E7 2 Complete Solvent Group Guide EZ Bio Complete Power Up Starting a Run Pre heat Pre cool Stage Changing the Run Setting Run Time During a run Pausing a Run Stopping a Run Shutdown Defrosting Out of Balance Inert Gas Purge SECTION 2 FAULT FINDING DIAGNOSTICS Safety Interlocks and Errors Audible Warnings Other Troubleshooting User Vacuum Test 04 5359 Issue 1 6 October 2008 Page i Genevac EZ 2 Series Personal Evaporator User Manual SECTION3 MAINTENANCE Chapter1 Software Introduction Safety and Maintenance Notes Special tools and equipment Preparation Uploading Testing Errors Completion ONOOARWN Chapter 2 Drive Belt 1 Introduction 2 Safety and Maintenance Notes 3 Special tools and equipment 4 Removal 5 Refitting 6 Testing 7 Completion
24. TART button 6 6 Ensure that pressure drops to below 500 mbar and continues to drop i When a new seal is fitted manual pressure may be required on the lid to assist to bed in the seal If pressure fails to drop check that seal is correctly located on chamber wall and repeat items 6 1 to 6 6 inclusive 6 7 Carry out required run ensure that desired pressure is achieved 7 Completion 7 1 Dispose of defective seal and packaging 04 5359 Issue 1 6 October 2008 Section 3 Chapter 6 Page 2 Genevac EZ 2 Series Personal Evaporator User Manual Chapter 7 Pump 04 5359 Issue 1 6 October 2008 Section 3 Genevac EZ 2 Series Personal Evaporator User Manual 1 Introduction This chapter provides guidance in the removal replacement and refitting of Pump 2 Safety and Maintenance Notes Ensure that safety and maintenance notes Page vi are complied with throughout this work instruction 3 Special tools and equipment Description Part number Quantity Pump 2 head 100 120 Volt 70 1117 S AR Pump 4 head 100 120 Volt AR Pump 2 head 230 Volt AR Pump 4 head 230 Volt AR PO PO ENSURE THAT EVAPORATOR IS ISOLATED FROM MAINS POWER SUPPLY ANY MAINTENANCE OR REPAIR OF THIS PRODUCT NOT DETAILED IN THIS MANUAL SHALL BE CARRIED OUT BY GENEVAC PERSONNEL OR APPROVED REPRESENTATIVES OF GENEVAC USING ONLY APPROVED SPARE PARTS CS Traces of solvents may be present that could be harmful to
25. UCTIVE POLLUTION ANY MAINTENANCE OR REPAIR OF THIS PRODUCT NOT DETAILED IN THIS MANUAL SHALL BE CARRIED OUT BY GENEVAC PERSONNEL OR APPROVED REPRESENTATIVES OF GENEVAC USING ONLY APPROVED SPARE PARTS Section 4 Page 3 Genevac EZ 2 Series Personal Evaporator User Manual Warranty Statement This product is guaranteed for period of 12 months from the date of delivery to site In the unlikely event of any defect arising due to faulty materials or construction resulting in system failure the unit will be repaired free of charge This to include all labour and component costs incurred This warranty is subject to the following provisions 1 2 8 System to be returned to Genevac in appropriate packaging for repair System must be sited installed and operated in accordance with operator instruction manual All vapours must be ducted away from the system as described in the installation instructions and operator manual Unit only used for purpose it was sold and in accordance with Genevac published solvent list Preventative maintenance to be adhered to as detailed in operator s manual When items are replaced by the customer Only Genevac approved parts are to be used Vacuum pump to be exchanged for refurbished unit in event of failure Owner responsible for exchange and return of failed unit Warranty does not cover accidental damage misuse or inappropriate repair by untrained personnel Failure to adhere
26. at can be harmful to your health or lead to material damage This symbol provides information about technical requirements which if not followed can lead to malfunctions inefficiency and reduced productivity This symbol indicates that there may be a risk to sample integrity gt H E gt 2 2 Safety Please be assured that sudden boiling of solvent within the condenser jar is normal and it will re condense You may also see ice forming in the jar this is normal under certain conditions The condenser jar is only to be emptied at the end of a run when the evaporator has stopped but not when DEF is displayed If ice forms in the Condenser jar allow it to thaw naturally Do not use implements to try and break the ice as this may damage the jar Cold water may be added to the jar to accelerate the thawing process BEFORE OPERATING THE SYSTEM IT IS IMPORTANT THAT THE FOLLOWING NOTES ARE READ TO ENSURE THAT THE IMPLICATIONS TO THE SAFETY OF PERSONNEL OPERATING THE SYSTEM AND FOR THE PROTECTION OF SAMPLE INTEGRITY ARE UNDERSTOOD CARE IS TO BE TAKEN WHEN REMOVING THE COLLECTION JAR AS IT IS HEAVY ESPECIALLY WHEN FULL OF SOLVENT INSPECT THE COLLECTION JAR BEFORE EVERY USE FOR DAMAGE CRACKS ETC DO NOT ALLOW TO FILL ABOVE MAX LINE AS IT MAY CAUSE DAMAGE TO THE PUMP AND STOP CONCENTRATION EMPTY THE COLLECTION JAR AFTER EVERY RUN REFER TO LIST OF ACCEPTABLE SOLVENTS BEFORE USE 04 5359 Issue 1 6 October 2008 Pag
27. condenser Shutdown Shutdown is when the pump and condenser is shutdown This can be manual or automatic Manual To shutdown simply press the Stop button when not in a run Automatic The system will also shutdown automatically if left unattended for 15 minutes at the end of arun This allows the evaporator to be loaded at the end of the day safe in the knowledge that it will turn itself off when done The display will clear to the software version and the LCD backlight will turn off Defrosting Defrosting is automatic although under certain conditions you may need to force an additional defrost cycle This can be done by pressing and holding down the PAUSE button for 5 seconds dEF will be displayed and the defrost cycle will last for 30 minutes You can cancel the defrost cycle by again pressing and holding down the PAUSE button for 5 seconds Out of Balance Should the evaporator be loaded incorrectly or go out of balance during a run the system will end the run automatically venting and bringing the rotor to rest safely The LCD will show Err BAL 04 5359 Issue 1 6 October 2008 Section 1 Chapter 3 Page 7 Genevac EZ 2 Series Personal Evaporator User Manual Inert Gas Purge The primary function of the Inert Gas Purge system is to control the supply of inert gas to the evaporating system during the evaporation of low flash point solvents such as diethyl ether to prevent the possibility of explosion Operation
28. de the tensioning tool tool between the belt and the elastic band at a position along the belt immediately adjacent to the tapped holes Ensure min gap between the tool shaft and the base of the system 4 13 Hold the tool such that a steady load can be applied to the rubber covered end i During item 4 14 the yellow end of the tool body should meet the mark of 3 5kg Do not hold the body of the tool 4 14 Compress the tool so that the small spindle is just touching the rubber band 4 15 If required adjust tension as detailed in item 5 04 5359 Issue 1 6 October 2008 Section 3 Chapter 2 Page 2 Genevac EZ 2 Series Personal Evaporator User Manual 4 16 If belt tension cannot be achieved replace belt as detailed in items 4 18 to 4 21 inclusive 4 17 If belt is broken remove belt and check condition of drive pulleys 4 18 Loosen the 4 drive motor securing screws maximum 3 turns 4 19 Loosen the lock nut on the adjuster screw 4 20 Rotate belt tension adjuster fully anticlockwise to loosen belt 4 21 Remove belt 5 Refitting 5 1 Ensure that the 4 drive motor securing screws are loosened 5 2 Ensure that belt tensioner is fully unscrewed anticlockwise 5 3 Position replacement belt over drive pulleys i During item 5 4 check belt tension with special tool 54 Screw in belt tension adjuster clockwise to tighten belt until correct tension is obtained 5 5 Tighten the 4 drive motor securing screws 5 6 Rotate be
29. e glass tubes with a wall thickness of no less than 1 2mm are recommended Do not use worn or scratched tubes or vials Do not load tubes or vials into sample holders other than those types that have been approved by Genevac Ltd Do not use sample holders that have not been supplied with this system without consulting Genevac Service 2 7 Lid operation The lid has an electrical lock and is opened and closed manually after pressing the LID unlock button There is a safety device fitted that prevents the lid from being unlocked and opened whilst the rotor is rotating or until the chamber pressure has equalised 2 8 Limitations of use correct operation Sample Holders and Swings are to be kept clean and free from contamination and corrosion j Accuracy of IR Sensor is dependant upon condition of Sample Holders and Swings To ensure Your evaporating system is unsuitable for use under the following circumstances e With strong mineral acids such as HCI and HBr at all concentrations unless specifically built to order EVAPORATING DIETHYL ETHER AND SIMILAR LOW AUTO IGNITION SOLVENTS WITHOUT A GENEVAC INERT GAS PURGE SYSTEM FITTED TO THE EVAPORATOR AND PUMP e For use as a pressure vessel 04 5359 Issue 1 6 October 2008 Page viii Genevac EZ 2 Series Personal Evaporator User Manual SECTION 1 GENERAL Chapter 1 UNPACKING AND SETTING UP 00 oe eee ee eter rene nan nc non nan nn nana anne r nan nn enana nn Chapter 2 QUICK ST
30. e run will terminate Setting Run Time The run time can be changed during a run should the need be to concentrate samples rather than dry them This can achieved in increments of 1 minute going up to hourly increments Press and hold the START button for 5 seconds the pressure indication will change to display SEt which will flash rapidly The Elapsed Time display will now indicate the proposed maximum run time By pressing the START button you can increase the time and by pressing the LID button you can decrease the time Once you have set the required run time release the buttons 5 seconds later the EZ 2 display will revert back to the current run status and the run will continue for the duration that you have just set All run duration changes are saved so that the next time that you select the same run the time that you previously set will be used Another feature of the software is the ability to adjust the maximum run time further If the previous procedure has been used then you can also adjust the maximum run time by increments of 10 minutes by momentarily pressing the START or LID buttons If the maximum run time is 2 hours or less then you can only adjust the time by 1 minute increments This adjustment is not saved so will not affect future runs 04 5359 Issue 1 6 October 2008 Section 1 Chapter 3 Page 5 Genevac EZ 2 Series Personal Evaporator User Manual EEE During a run Elapsed time will be incrementing as the
31. e vi Genevac EZ 2 Series Personal Evaporator User Manual Samples in the chamber are subjected to accelerations of up to 500G with a maximum load capacity of 1 5 kg per swing Do not place any objects on top of the evaporator during a run The following precautions should always be observed Ensure the catchpot or an alternative exhaust condenser is fitted prior to operation of the system Solvents condense in the condenser jar and catchpot and any exhaust condenser when used These solvents should be disposed of appropriately Solvent vapour is present in the exhaust line this line should be run to a safe extraction source This is particularly important if HCI or other acids are being used Solvents can condense in the exhaust line and suitable precautions should be taken when working on the EZ 2 This is particularly important if HCI or other acids have been used 2 3 Collection jar fitting and removing To fit the jar position so that the handle is to the front lift the jar and rotate to the right a quarter turn until resistance is felt The collection jar is removed by rotating the jar to the left a quarter turn e Always ensure that the condenser jar is emptied before every run e Clean the condenser jar at regular intervals 2 4 Cleaning Clean the lid lid seal exterior paintwork and inside of the chamber with a soft lint free cloth using one of the following Detergent solution e g fairy liquid Bleach sol
32. ed in Technical Specification DURING RUN Err Lid Closed indicating Not Closed Operate lid Unlock button Lid mid run Open and re close lid if required Err Lid Locked indicating Not Locked Operate lid Unlock button Loc mid run Open and re close lid if required Err Out Of Balance Trip Check samples balanced to within 10g bAL Err Failure to pull a Vacuum Check lid seal UAC1 Check pump connections if disturbed Err Vacuum fails mid run Check lid seal UAC2 Check pump connections if disturbed MISCELLANEOUS run Rotary Run Selector Switch in Ensure Rotary Run Selector Switch is in SEL incorrect position correct position OPn Lid Unlock activated Open Lid Lid Err Tried to open lid before it is unlocked Reset and try again this time listen for 06 solenoid unlocking before lifting lid Err Lid closed switch stuck in closed Consult Genevac for advice 59 position PrG LO and PrG HI are only displayed on an EZ 2 with the Inert Gas Purge IGP option fitted All error messages can be cancelled by holding the Stop button down for 5 seconds 04 5359 Issue 1 6 October 2008 Section 2 Page 1 Genevac EZ 2 Series Personal Evaporator User Manual Audible Warnings Critical Error User Warning Shutdown Errors Audible warning format Series of 4 long beeps as run ends Repeats every 5 minutes until any user interaction Button pressed Run select switch moved Lid opened
33. elow 900mbar at start of a run but takes longer than usual to achieve a level of vacuum Condenser jar seal damaged Carry out user vacuum test to verify problem Lamp glass cracked Stop run and inspect replace lamp glass assembly Condenser jar full of volatile solvent Ensure condenser jar is emptied after every run Condenser fuse failed Consult Genevac for advice Lamp glass seal damaged Carry out user vacuum test to verify problem Vacuum stops pulling down at 215mbar 115mbar and or 30mbar System waitng for condenser to cool This is correct and no action is required It ensures maximum solvent recovery and pump life Boiling of solvent in condenser No problem This occurs normally Lid will not open despite OPn Lid displayed Vent blocked Remove blockage Lid seal stuck to glass due to excessive solvent on the lid seal Allow the solvent to evaporate before further use Clean seal and lid 04 5359 Issue 1 6 October 2008 Section 2 Page 2 Genevac EZ 2 Series Personal Evaporator User Manual Other Troubleshooting Continued SYMPTOM Lid glass is loose CAUSE The lid is designed so that the glass floats this makes it feel loose ACTION This is correct an no action is required Runs take longer than expected Lamp failure Check lamp is illuminated by looking through lid glass and replace if failed
34. ent to this image AUTO STOP WHEN DRY COUNTDOWN TIMER until the countdown completed This feature ensures that the EZ 2 is in a safe mode for continued operation There could have been a power failure or power fluctuation in the lab that caused the EZ 2 to reset i Please note that the countdown timer counts down from 96 to 0 Do not press the START button When the countdown timer reaches 0 the system will automatically enter the normal operation mode Starting a Run The LCD display then becomes illuminated showing pressure temperature and an elapsed time of 00 00 gee i Note that the lid will remain locked when there is no power to the system 04 5359 Issue 1 6 October 2008 Section 1 Chapter 3 Page 3 Genevac EZ 2 Series Personal Evaporator User Manual Refer to Page vi Paragraph 2 Safety and Maintenance Notes To open the lid press LID button then open the lid The lid remains unlocked for 10 seconds once the LCD indicates OPn Lid AUTO STOP WHEN DRY Place your samples into the EZ 2 Close the lid Select desired Solvent Type from rotary selector Select the required rotor temperature on the control knob Press START button i When the start button is pressed the system will check that the lid is closed and locked The condenser will start The pump starts when a vacuum is applied See the paragraph on Lid Messages to view displays Pre heat Pre cool Stage Preheating a
35. igh out of balance Ensure balanced to avoid vibration Excessive noise Incorrectly fitted pump Remove and refit pump Condensation inside chamber Incorrect run selected Check that the run selected is most appropriate Run changed after run started Avoid doing this as the system will have skipped the pre heat Cannot unlock and open lid lid LED not lit Spin down timer still active Wait until timer reaches zero then try again Lid button pressed too often in 5 minutes Wait for system to re enable lid open mechanism LED will re light i If action fails to resolve problem contact Genevac User Vacuum Test Start Aqueous run on EZ 2 Medium Boiling Point run on EZ 2 or H2O run on EZ Bio 04 5359 Issue 1 6 October 2008 Section 2 Page 3 Genevac EZ 2 Series Personal Evaporator User Manual Chapter 1 Chapter 2 Chapter 3 Chapter 4 Chapter 5 Chapter 6 Chapter 7 Chapter 8 SECTION 3 MAINTENANCE Software Drive Belt Fuses Lamp Unit Lamp Glass Assembly Lid Seal Pump Pump Head Components To request your spares and consumables online click on the link below or copy and paste it into your browser http www genevac com contact ezspares html 04 5359 Issue 1 6 October 2008 Section 3 Genevac EZ 2 Series Personal Evaporator User Manual Chapter 1 Software 04 5359 Issue 1 6 October 2008 Section 3 Genevac EZ 2 Series
36. ing on page vii of this manual Refitting Unpack replacement lamp glass assembly Check that lamp glass assembly chamber seal is correctly located Locate lamp glass assembly in to EZ 2 Refit securing screw Refit lamp unit as detailed in Chapter 4 items 5 2 to 5 5 inclusive 04 5359 Issue 1 6 October 2008 Section 3 Chapter 5 Page 2 Genevac EZ 2 Series Personal Evaporator User Manual EEE 6 Testing 6 1 Foran EZ 2 select AQUEOUS 6 2 Foran EZ 2plus select Medium Boiling Point 63 Foran EZ Bio select H2O 6 4 Set temperature to 60 C 6 5 Press START button 66 Ensure that pressure drops to below 500 mbar and continues to drop i If pressure fails to drop check that lamp glass assembly to chamber seal is correctly located and repeat items 6 1 to 6 6 inclusive 6 7 Carry out required run ensure that desired pressure is achieved 6 8 Check that lamp illuminates 7 Completion 7 1 Dispose of defective lamp glass assembly and packaging 04 5359 Issue 1 6 October 2008 Section 3 Chapter 5 Page 3 Genevac EZ 2 Series Personal Evaporator User Manual Chapter 6 Lid Seal 04 5359 Issue 1 6 October 2008 Section 3 Genevac EZ 2 Series Personal Evaporator User Manual 1 Introduction This chapter provides guidance in the removal replacement and refitting of Lid Seal 2 Safety and Maintenance Notes Ensure that safety and maintenance notes Page vi are complied with throughout
37. leaving the machine in pause mode for longer than 5 minutes You can restart the run by pressing the PAUSE button again 04 5359 Issue 1 6 October 2008 Section 1 Chapter 3 Page 6 Genevac EZ 2 Series Personal Evaporator User Manual Stopping a Run This can be manual or automatic Manual Press the Stop button while in the run The system will vent and then the rotor will be brought to a halt Automatic Without any user input the run will be automatically terminated when the system senses that the samples are dry It will do this by monitoring the amount of Coolheat required to keep the samples at their pre programmed temperature during evaporation When no further heat is required the samples will be subjected to a final drying phase where evaporation continues this is dependant on the run selected The system will then vent and the rotor will be brought to a halt so ending the run For situations where very little heat is required and the above method is inappropriate the system will run for a pre programmed time The time required depends on run selected and control temperature See Section 1 Chapter 3 Under both conditions the samples are perfectly safe Once the run has ended the system beeps 3 times and flashes the Lid button to attract attention to the end of run When the run has stopped the message dEF will be seen flashing on the LCD display this is quite normal and indicates the automatic defrost operation of the
38. locked during run Open and re close lid Err bAL Out Of Balance Trip Check samples balanced to within 10g run SEL Rotary Run Selecter Switch in incorrect position Ensure Rotary Run Selecter Switch is in correct position Err UAC Failure to pull a Vacuum or Check lid seal Vacuum fails mid run Refer to Section 2 of Owners Manual Clear error by pressing and holding i for 5 seconds 04 5359 Issue 1 6 October 2008 Section 1 Chapter 2 Page 3 Genevac EZ 2 Series Personal Evaporator User Manual Chapter 3 DETAILED OPERATION 04 5359 Issue 1 6 October 2008 Section 1 Genevac EZ 2 Series Personal Evaporator User Manual SOLVENT LIMITATIONS Evaporation of Acid Chlorides The solvent compatibility of your EZ 2 Series evaporator is indicated on the lid If NOT HCl COMPATIBLE VERSION is indicated then do not attempt to evaporate this class of solvent in your system If HCl COMPATIBLE is indicated your system is compatible with HCI Set the Solvent Group Selector to Aqueous and press Start Evaporation of Di ethyl ether Genevac supply an optional inert gas purge system this is mandatory if Di ethyl ether is to be evaporated safely Evaporation of High Boiling Point Solvents Genevac does not recommend the EZ 2 Series for use with solvents whose boiling points at atmospheric pressure are 170 C or above This includes DMSO DMI and NMP Solvent Group Guide EZ 2 amp E7 2 Quick G
39. lt one complete revolution 5 7 Recheck belt tension and adjust accordingly 5 8 Tighten lock nut on adjuster screw against chassis 5 9 Recheck belt tension and adjust accordingly 5 10 Remove rubber band 6 Testing 6 1 Ensure that rotor rotates freely by hand 7 Completion 7 1 Replace belt access panel 7 2 Refit securing screws 7 3 With assistance carefully stand the system back upright on it s feet Once upright it is acceptable to open the lid again 74 Refit drip plate catchpot and condenser jar 7 5 Reconnect mains power lead 04 5359 Issue 1 6 October 2008 Section 3 Chapter 2 Page 3 Genevac EZ 2 Series Personal Evaporator User Manual Chapter 3 Fuses 04 5359 Issue 1 6 October 2008 Section 3 Genevac EZ 2 Series Personal Evaporator User Manual 1 Introduction This chapter provides guidance in the removal replacement and refitting of Fuses FS6 FS7 ee T16A T16A SUPPLY 100V 50Hz VENT INLET 2 Safety and Maintenance Notes Ensure that safety and maintenance notes Page vi are complied with throughout this work instruction 3 Special tools and equipment Description Part number Quantity 100 Volt 50Hz System F6 Main Supply 16 0A 1 F7 Main Supply 16 0A 1 100 Volt 60Hz System ae coc F6 Main Supply 16 0A 1 F7 Main Supply 16 0A 1 120 Volt System Sa F6 Main Supply 10 0A 1 F7 Main Supply 10 0A 1 230 240 Volt System EA F6 Main Supply 6 3A 1 F7
40. lvent Group Guide EZ Bio Complete SOLVENT ABBREVIATION BEST PROGRAM TO USE Acetic acid HOAc EtOOH H20 Acetonitrile ACN MeCN BP lt 75 Ammonia NH3 H20 NH OH Dimethylamine DMA BP lt 50 Chloroform TCE Tri chloroethane BP lt 50 1 2 dichloroethane DCE H20 Dichoromethane DCM methylene chloride BP lt 50 1 4 Dioxane 1 4 Diox BP lt 75 Ethanol EtOH H20 Ethyl Acetate EtAc AcOEt BP lt 75 Heptane Hept H20 Hexane Hex BP lt 75 Isopropanol IPA BP lt 75 H20 Methanol MeOH BP lt 75 Pyridine Py H20 Trifluoroacetic acid TFA MIXTURE BP lt 75 Tetrahydrofuran THF BP lt 50 BP lt 75 Toluene PhMe H20 Water H20 H20 Water and Ammonia H20 NH3 H20 NH OH Water and Acetonitrile H20 MeCN H20 BP lt 75 Water and Methanol H20 MeOH H20 BP lt 75 Note that where there are two programs listed the first will give better solvent recovery while the second may give slightly faster drying 04 5359 Issue 1 6 October 2008 Section 1 Chapter 3 Page 2 Genevac EZ 2 Series Personal Evaporator User Manual Power Up The LCD See Display is made up of 110 segments and when the system is switched ON it will illuminate for 1 second so that the you have time to observe that there are no segments missing The display will then clear and display Sys Test for a few seconds before displaying the software version Version displayed may be differ
41. nd cooling is automatically selected when required If the chamber is not at or sufficiently close to the required temperature when the start button is pressed then the system will enter pre heat or pre cool mode Pre heat is sometimes required to avoid condensation on the inside of the chamber Pre cool is sometimes required to avoid bumping of volatile solution AUTO STOP WHEN DRY If the system enters pre heat pre cool mode then the rotor will spin up The pre heat cool temperature is determined by the run selected 04 5359 Issue 1 6 October 2008 Section 1 Chapter 3 Page 4 Genevac EZ 2 Series Personal Evaporator User Manual Changing the Run You are able to change the run type at any time even during a run The system will automatically re program the system to the new settings The new run is only accepted after a 3 second thinking time incase you change your mind and re select the original run Acceptance is confirmed by a short beep The state of the Dri Pure LED indicates if Dri Pure is used in the run indicated Max Sample temperature can be adjusted throughout the run and will be accepted immediately Irrespecitve of run selection elapsed time will not be reset If the new run is a two stage run and the time already elapsed is greater than the 1 stage time then the run continues with the 2 stage settings If the maximum allowable time for the new run is shorter than the time already elapsed then th
42. nt For an EZ Bio select H20 Press START button IF PUMP LINES ARE INCORRECTLY FITTED THE PUMP COULD PRESSURISE THE SYSTEM CAUSING DAMAGE TURN SYSTEM OFF IF PRESSURE INCREASE OCCURS DURING ITEM Ensure that pressure drops to below 500 mbar and continues to drop If pressure fails to drop repeat items 4 1 to 4 3 inclusive and check condition of PTFE hoses and connections repeat items 6 1 to 6 7 inclusive Carry out required run ensure that desired pressure is achieved Completion Fit hose blanking caps to defective pump Place pump into packaging for despatch back to Genevac Return to Genevac 04 5359 Issue 1 6 October 2008 Section 3 Chapter 7 Page 3 Genevac EZ 2 Series Personal Evaporator User Manual Chapter 8 Pump Head Components 04 5359 Issue 1 6 October 2008 Section 3 Genevac EZ 2 Series Personal Evaporator User Manual 1 Introduction This chapter provides guidance in the removal replacement and refitting of Pump Head Components 2 Safety and Maintenance Notes Ensure that safety and maintenance notes Page vi are complied with throughout this work instruction 3 Special tools and equipment Description Part number Quantity 2 Head Pump Head Service Kit 04 3715 AR 4 Head Pump Head Service Kit 04 3716 AR Pulley Spanner 44 3 3mm AC2370 1 Allen Key 4mm AB9603 1 Spanner Open Ended 17mm 1 ENSURE THAT EVAPORATOR IS ISOLATED FROM MAINS POWER SUPPLY
43. nual MODEL Mechanical Data Max RPM Max G Drive system Sample load including swings Max imbalance IR lamps number Vacuum system Pressure display Automatic Pressure control Bump protection System ultimate pressure Auto vacuum vent valve Temperature controls Temperature control range Temperature control accuracy Temperature sensing Temperature display range Chamber temp range End of run determination Condenser Data Condenser temperature Vacuum pots number Useable Capacity vacuum pot Materials condenser jar Refrigerant Solvent compatibility Boiling points HCI compatibility Diethyl Ether compatibility Dimensions Width Without Catchpot With Catchpot Depth Height Weight Inert Gas supply Max pressure Min pressure Flow 30 litres min STP Purge system filter draining 04 5359 Issue 1 6 October 2008 EZ 2 2150 500G Direct 1 5 kg 40g 0 1150 mbar 10 mbar atm Yes 10 mbar Yes Ambient to 80 C 2 5 C infra red pyrometer 99 to 99 C Ambient Automatic Timed 40 C 1 1 litre Borosilicate glass R404a Up to 120 C Only with option Only with option 570 mm 597 mm 645 mm 700 mm 82 kg 1 5 bar 1 0 bar Automatic Ez 2Pus 2150 500 G Direct 1 5 kg 40g 0 1150 mbar lt 2 mbar atm Yes 2 mbar Yes Ambient to 80 C 2 5 C infra red pyrometer 99 to 99 C Ambient to 80 C Automatic Timed 40 C 1 1 litre
44. onsult Genevac Service if you intend to use an alternative nert gas 04 5359 Issue 1 6 October 2008 Section 1 Chapter 3 Page 8 Genevac EZ 2 Series Personal Evaporator User Manual SECTION 2 FAULT FINDING DIAGNOSTICS Safety Interlocks and Errors Audible Warnings User Vacuum test Trouble shooting 04 5359 Issue 1 6 October 2008 Section 2 Genevac EZ 2 Series Personal Evaporator User Manual Safety Interlocks and Errors If an error occurs during a run then one of 2 things will happen 1 The run will be terminated This means that the EZ 2 has detected a failure in something that may have an effect on Sample Integrity The physical condition of the evaporator Safety Critical An error code will be displayed when the rotor has stopped spinning and the pressure is at atmosphere 2 The run will continue in a reduced features mode This means that the EZ 2 has detected a failure in something that does not have any effect on Sample Integrity The physical condition of the evaporator Safety Critical An error code will be displayed when the rotor has stopped spinning and the pressure is at atmosphere INDICATION CAUSE OF ERROR RECTIFICATION AT START PrG Low inert gas pressure Check Gas supply ensure within limits LO Inert gas supply switched OFF specified in Technical Specification PrG High inert gas pressure Check Gas supply ensure within limits HI specifi
45. run progresses HH MM The run progress indicator Test tube will show that the run is in progress by repeatedly lighting the solvent vapour icon EVAPORATION ICON 80 20 There are 9 segments within the test tube when the top one is extinguished it indicates that the run is 80 complete the next 7 indicate progress through the the last 20 of the run Note that these are for guidance only and give no real timing information The sample pellet icon in the bottom of the test tube flashes when the run has finished The Dri Pure LED indicator will be lit during any Dri Pure enabled run During the Dri Pure part of a run the word Ramping will appear below Pressure on the LCD display During certain runs the message dEF will be seen flashing on the LCD display this is quite normal and indicates the automatic defrost operation of the SpeedTrap Pausing a Run Press the Pause button COUNTDOWN TIMER System will vent and stop the rotor coundown timer will count from 96 down to 0 The lid can then be opened and the samples examined The run can be re started within the next 5 minutes by closing the lid and pressing Start If Dri Pure had finished before the pause button was pressed then Dri Pure will not be re applied During the 5 minutes wait for a restart the elapsed time indicator will be counting down in MM SS from 05 00 mins Pause mode can be cancelled by pressing Stop while paused or by
46. stems prevent bumping and overheating of delicate samples whilst ensuring a smooth fast evaporation of the solvent even with difficult mixtures A wide selection of popular sample holders accommodate most common sample formats including round bottom flasks up to 500ml tubes up to 160mm long custom reaction blocks and of course shallow or deep well microplates For real peace of mind with thermally labile samples the EZ 2 Series automated dryness detection and auto shutdown software is always guarding your samples Sample evaporation has never been easier or quicker 04 5359 Issue 1 6 October 2008 Page v Genevac EZ 2 Series Personal Evaporator User Manual 2 Safety and Maintenance Notes 2 1 Symbols The following safety symbols are used throughout this manual and can be found on the equipment The definitions and scope of each symbol is as described below WARNING THIS SYMBOL INDICATES HAZARDS THAT CAN LEAD TO SERIOUS MATERIAL DAMAGE OR POTENTIAL SERIOUS INJURY THIS SYMBOL MEANS THAT YOU SHOULD NOT TOUCH SURFACES IN THESE AREAS THESE SURFACES CAN BE VERY HOT AND MAY LEAD TO BURNS This symbol indicates that you should refer to the relevant part of the User Manual for additional information SERIOUS MATERIAL DAMAGE OR POTENTIAL SERIOUS INJURY AND THAT THESE 2 SYMBOLS COMBINED INDICATES HAZARDS THAT CAN LEAD TO YOU SHOULD READ THE RELEVANT PART OF THE USER MANUAL This symbol provides information about hazards th
47. ted by pressing the gt button to increase the duration and by pressing the a button to decrease the duration OMONOOARWN gt Evaporation of HCI Thionyl Chloride or other acid chlorides The solvent compatibility of your EZ 2 evaporator is indicated on the lid If NOT HCl COMPATIBLE is indicated then do not attempt to evaporate this class of solvent in your system If HCl COMPATIBLE is indicated your system is compatible with HCI Set the Solvent Group Selector to Aqueous and press i Evaporation of Di ethyl ether Genevac supply an optional inert gas purge system this is mandatory if Di ethyl ether is to be evaporated safely SOLVENT GROUP GUIDE EZ 2 8 EZ 2 Quick GUIDE Solvent Description Boiling point range Examples of solvents at atmosphere group Volatile solvents where bumping is Low BP 40 90 C DCM through to Pyridine not expected v BP Mixtures of volatile solvents 40 909 C DEM Methanol mixture ixture bumping can be expected Water based where freezing at 0 Aqueous C is anticipated but bumping is not N A Water expected Medium Bp Less volatile solvents generally 90 155 C Ethyl Acetate through to DMF bumping is not expected Mixtures with a volatile solvent that ae N 10 PUD e 40 155 C DMF Methanol mixture mixture volatile solvent that requires low pressure to evaporate HPLC Water Acetonitrile or Water 60 to 100 C Water Methanol or Fraction
48. the condenser and lead away to the laboratory fume extraction system This will ensure that any residual vapour which passes through the condenser and pump does not cause damage to your system Er To_maintain a solvent vapour free environment around your EZ 2 we suggest that you 12 Connect mains supply lead supplied 04 5359 Issue 1 6 October 2008 Section 1 Chapter 1 Page 1 Genevac EZ 2 Series Personal Evaporator User Manual Consumer Packaging 1 Before signing for package or before opening case check for damage If there is contact Genevac service UK Service 44 0 1473 243000 USA Service 1 845 267 2211 2 Remove clips that retain the lid and remove lid 3 Check contents against packing list 4 Remove the screws from the timber sleeve and carefully lift clear 5 Position base and EZ 2 near to operating bench location 6 With assistance 3 people total lift EZ 2 from base using hand holds and position where required on to bench LEFT HAND SIDE HAND HOLDS RIGHT HAND SIDE HAND HOLDS 04 5359 Issue 1 6 October 2008 Section 1 Chapter 1 Page 2 Genevac EZ 2 Series Personal Evaporator User Manual 7 Ensure that there is 50mm clearance from edge of bench and other equipment The system can be easily moved by placing paper or similar under feet If this is employed ensure all traces are removed on completion 50mm 50mm 2 2 8 Remove accessories containers and
49. then load code data from key storing at the appropriate destination with the Hour Glass symbol animated in the top right hand corner of the LCD display During upload the system displays LoAd codE This process can take up to 34 When the rSt SYS message is displayed 5 3 1 Switch off 5 3 2 Remove the software Data Key 5 3 3 Refit blanking cap onto connector 5 3 4 Switch back on the new code is then used by the system Testing No formal testing is required Errors If any errors are observed and or the evaporator fails to operate make a note of the error code s displayed and contact Genevac service Completion Place software Data Key in a safe and secure place 04 5359 Issue 1 6 October 2008 Section 3 Chapter 1 Page 2 Genevac EZ 2 Series Personal Evaporator User Manual Chapter 2 Drive Belt 04 5359 Issue 1 6 October 2008 Section 3 Genevac EZ 2 Series Personal Evaporator User Manual 1 Introduction This chapter provides guidance in the removal and refitting of Drive Belt BELT TENSION DRIVE BELT BELT TENSIONER DRIVE MOTOR SECURING SCREWS 2 Safety and Maintenance Notes Ensure that safety and maintenance notes Page vi are complied with throughout this work instruction 3 Special tools and equipment Description Part number Quantity DRIVE BELT 04 2770 1 BELT TENSIONING TOOL 04 3658 1 1 ENSURE THAT EVA
50. this work instruction 3 Special tools and equipment Description Part number Quantity LID SEAL 04 5094 S 1 ANY MAINTENANCE OR REPAIR OF THIS PRODUCT NOT DETAILED IN THIS MANUAL SHALL BE CARRIED OUT BY GENEVAC PERSONNEL OR APPROVED REPRESENTATIVES OF GENEVAC USING ONLY APPROVED SPARE PARTS CS Traces of solvents may be present that could be harmful to your health_or lead to material damage There may be a risk to sample integrity if required vacuum is not achieved 04 5359 Issue 1 6 October 2008 Section 3 Chapter 6 Page 1 Genevac EZ 2 Series Personal Evaporator User Manual 4 Removal 4 1 Switch evaporator ON 4 2 Press LID OPEN button 4 3 Open lid ST Check for traces of solvent on seal and wipe clean before removing seal c E The seal material may absorb significant quantities of some solvents Do not use tools they may damage the chamber 44 Manually remove seal from chamber by carefully pulling away from chamber working on a 200mm section at a time Once one comes out the remainder is removed easily 5 Refitting 5 1 Unpack replacement lid seal 5 2 Position on top of chamber wall with lip pointing inwards towards centre 5 3 Work firmly into position using only fingers ensuring the seal is fully home 6 Testing 6 1 Close lid 6 2 Foran EZ 2 select AQUEOUS 63 For an EZ 2 select Medium Boiling Point 64 Foran EZ Bio select H20 6 5 Press S
51. uction Using the most advanced proven technology in evaporation science the EZ 2 Series has been designed specifically for solvent removal in life science research be that concentration of samples or complete drying Genevac s fifteen years of experience in vacuum engineering has resulted in the creation of a compact sample concentrator combining great performance ease of use greater than a rotary evaporator and compatibility with all commonly used solvents and acids The innovative design of the EZ 2 presents real advantages within parallel synthesis and medicinal chemistry as well as for general purpose solvent removal needs in life science laboratories At the heart of the evaporator are a robust solvent resistant oil free pump from ILMVAC and the high efficiency defrost free SpeedTrap designed by Genevac engineers which traps all solvents as liquids even water There are three EZ 2 models Standard Plus and Bio e The Standard EZ 2 is suitable for water and volatile solvents e The Plus E7 2 is recommended for more difficult solvents with higher boiling points as it is equipped with chamber heating and a more powerful pump Either model can be specified with resistance to chlorinated acids e The Bio EZ Bio is suitable for low to medium boiling point solvents in the range up to 120 C There are pre programmed methods for DNA purification and extraction oligosynthesis protein purification and removing aqueous mixtures
52. uide Description Boiling point range Examples of solvents at atmosphere Volatile solvents where bumping is not Low BP 40 90 C DCM through to Pyridine expected Low BP Mixtures of volatile solvents bumping 40 902 C DCM Methanol mixture Mixture can be expected sF Aqueous Water based where freezing at 0 C is N A Water anticipated but bumping is not expected Medium BP Less volatile solvents generally go 4sgoc Ethyl Acetate through to DMF bumping is not expected Mixtures with a volatile solvent that is Med Low prone to bumping with a less volatile BP mixture solvent that requires low pressure to 40 155 C DMF Methanol mixture evaporate HPLC Water Acetonitrile or Water Methanol 60 to 100 C Water Methanol or Fraction fractions Water Acetonitrile 500G Centrifuge at atmosphere with temperature control Available on EZ 2 only Maximum Run Times The maximum run times are specified in the table below These times have been calculated assuming the maximum volume of solvent is present in the system The maximum run time is affected by the Program setting and the maximum temperature selected for the run Run Selector ASSUMED MAXIMUM TIME APPROPRIATE FOR TEMPERATURE SETTING Setting Lamp Off 30 39 C 40 49C 50 59 C 60 69C 70 80C Mae Low BP 617 309 309 309 309 309 Low BP Mixture 550 275 275 275 275 275 Aqueous 2111 990 791 565 495 49
53. ution if using biological agents On inside of chamber and underside of lid ONLY Methanol Ethanol 2 5 Genevac Evaporators and Combustible Solvents Please note it remains the responsibility of the user to consider safety when evaporating any combustible solvents and ensure the system is placed in a well ventilated environment Genevac s position regarding evaporation of such solvents particularly with respect to the European ATEX directive is available on our website or from your local sales representative 04 5359 Issue 1 6 October 2008 Page vii Genevac EZ 2 Series Personal Evaporator User Manual 2 6 Safe loading of rotor Never exceed the maximum load capacity of 1 5 kg per swing Balance sample holders to within 10g approximately Locate tubes correctly in tube holders Load tube holders in balanced configurations Distribute tubes in sample holders symmetrically in both directions Genevac Ltd will accept no responsibility for any loss or damage incurred by improperly or excessively loaded rotors Locate sample blocks correctly in sample swings Before starting or restarting a run check the following Ensure that both sample holders swing freely by swinging them by hand after loading The white PTFE washers located on each rotor pin are in place and in good condition All tube plates are correctly located Use only tubes that are able to withstand significant loads Medium or thick wall Borosilicat
54. your health_or lead to material damage There may be a risk to sample integrity if required vacuum is not achieved gt 04 5359 Issue 1 6 October 2008 Section 3 Chapter 7 Page 1 Genevac EZ 2 Series Personal Evaporator User Manual 4 Removal 4 1 Disconect mains power supply 4 2 From rear of evaporator undo the securing screws qty 4 that secure pump tray assembly i Do not subject the PTFE hoses and cables to undue strain 4 3 Carefully withdraw pump tray assembly FASTENERS PTFE HOSES POWER CONNECTOR Fig 1 4 Head Pump 44 Disconnect pump power connector 4 5 Carefully note position of the 2 PTFE hoses and disconnect from pump 5 Refitting 5 1 Unpack replacement pump and retain packaging 5 2 Remove blanking caps from the two hoses and retain 5 3 Position pump assembly and connect PTFE hoses to positions noted at item 4 5 54 Connect pump power connector i During item 5 5 ensure that the PTFE hoses and cables are not trapped or distorted 5 5 Carefully slide pump assembly into back of evaporator 56 Secure with the screws qty 4 04 5359 Issue 1 6 October 2008 Section 3 Chapter 7 Page 2 Genevac EZ 2 Series Personal Evaporator User Manual EEE 6 6 1 6 2 6 3 6 4 6 5 6 6 6 7 6 8 6 9 7 1 7 2 7 3 Testing Reconnect mains power supply Power up evaporator For an EZ 2 select AQUEOUS For an EZ 2plus select Medium Boiling Poi

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