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VK8000 with BIO-DIS.book

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1. Varian Inc Revision D 01 06 VK 8000 for use with Page 21 P N 70 9039 Apparatus 3 Apparatus 7 Operator s Manual Unpacking and Positioning Unpacking Your Peristaltic Pump we Step 1 Carefully remove the peristaltic pump from its shipping carton Remove all cushioning and tape Step 2 Place the peristaltic pump on a clear dry level section of the benchtop between the VK 8000 and the Apparatus 3 Apparatus 7 Varian Inc Page 22 VK 8000 for use with Revision D 01 06 Unpacking and Positioning Apparatus 3 Apparatus 7 Operator s Manual P N 70 9039 Unpacking Your Syringe Pump The syringe pump is shipped in individual packaging Step 1 Carefully lift the unit out of the box Step 2 Remove any cushioning material Step 3 Place the unit on a clear dry level section of the benchtop between the VK 8000 and the Apparatus 3 Apparatus 7 Varian Inc Revision D 01 06 VK 8000 for use with Page 23 P N 70 9039 Apparatus 3 Apparatus 7 Operator s Manual Unpacking and Positioning Unpacking Your Filter Changer The filter changer is shipped in individual packaging Step 1 Carefully lift the unit out of the box Step 2 Remove any cushioning material Step 3 Place the unit on a clear dry level section of the benchtop between the VK 8000 and the syringe pump Varian Inc Revision D 01 06 VK 8000 for use with P N 70 9039 Page 24 Apparatus 3 Ap
2. Varian Inc Page 32 VK 8000 for use with Revision D 01 06 Setting Up Apparatus 3 Apparatus 7 Operator s Manual P N 70 9039 This page was intentionally left blank except for this message Varian Inc Chapter 6 Setting Up Your VK 8000 with Syringe Pump and Filter Changer Cable Connections Warning The electrical connection at the back of the VK 8000 is the primary disconnect for the instrument The instrument should be positioned to allow accessibility to the power cords for easy disconnection Refer to the example diagram see Figure 3 Cable Connections for the VK 8000 Syringe Pump Filter Changer and Apparatus 3 on page 34 to correctly make cable connections for this configuration Varian Inc Page 34 VK 8000 for use with Revision D 01 06 Setting Up Apparatus 3 Apparatus 7 Operator s Manual P N 70 9039 FIGURE 3 Cable Connections for the VK 8000 Syringe Pump Filter Changer and Apparatus 3 APPARATUS 3 FILTER CHANGER SYRINGE PUMP Step 1 Connect the single end of a Y cable 1 P N 5075 0446 to the RS232 port on the filter changer Step 2 Connect an RS232 cable 2 P N 5075 0252 between the RS232 port on the VK 8000 and one extension of the Y cable attached to the filter changer Step 3 Connect a nine pin cable 3 P N 5075 0449 to the remaining extension of the Y cable attached to the filter changer Step 4 Connect
3. CONFIGURE NEXT OK Press NEXT to avoid configuration CUSTOM NEXT OK Press OK to enter custom mode CUSTOM MENU PRINT CUSTOM SETUP NEXT OK Press NEXT AUTO SEQ NO NEXT OK Press OK ZERO NEXT OK Press OK POUND SIGN NEXT OK Press OK _ UNDERSCORE NEXT OK Press OK ONLINE OFFLINE YES NEXT OK Press OK EXT CH SET NO NEXT OK Press OK PRINT READY YES NEXT OK Press NEXT PRINT READY NO NEXT OK Press OK READY Your printer is now configured correctly Varian Inc chapter 10 Service and Warranty The warranty is provided by Varian Inc or one of its authorized representatives Service and Warranty Information Varian dissolution products carry a one year warranty on parts and labor The Dissolution Systems Service Department or one of its representatives will at its option either repair or replace any mechanical and electrical components in your instrument which prove to be defective During the first year of warranty coverage there is no charge for the labor to repair your unit The Dissolution Systems Service Department or one of its representatives will determine the best site to repair the unit either onsite or returned to Varian Inc Any onsite warranty services are provided only at the initial installation point Installation and onsite warranty services are available only in Dissolution Systems service travel areas Varian Inc Page 102 VK 8000 fo
4. Step 4 Replace the tray under the filter changer by sliding it forward along the tracks until it stops Varian Inc Page 96 VK 8000 for use with Revision D 01 06 Maintenance Apparatus 3 Apparatus 7 Operator s Manual P N 70 9039 Step 5 Replace the filter catch basin Report Center Impact Printer The following is helpful information for using your impact printer Installing the Cartridge Ribbon If the printer is used infrequently the print impression sometimes becomes weak because the ribbon dries out If the printed material is difficult to read and you suspect this is the cause of the problem advance to a properly inked portion of the ribbon by pressing the printer toggle switch into the Paper feed position If the printing is still faint replace the cartridge To install the cartridge complete the following steps Step 1 Toggle the printer off line by pressing the printer toggle switch to the OnLine Off Line position When the printer is off line the Ready LED does not illuminate Step 2 Four small grooves are embossed on the printer cover Gently push on these grooves to tilt the cover When the printer cover is tilted up you can lift it off completely Step 3 Push down on the right side of the ribbon cartridge marked PUSH and remove the old cartridge Step 4 Install the new cartridge If there is already paper in the printer hold the cartridge between your thumb and index finger slide it over
5. on page 67 e To correct an entry press BACKSPACE to go to the previous character position and re enter the correct value e If ashorter sample timepoint than the previous one is entered for a given row the row number will not increment Times must increase from one row to the next Varian Inc Revision D 01 06 VK 8000 for use with Page 67 P N 70 9039 Apparatus 3 Apparatus 7 Operator s Manual Operating the VK 8000 e To shorten a saved program enter 000 00 for the first sample timepoint you wish to discard All successive timepoints are eliminated e Press ESC to save all new values and return to the Program Variables screen Note Sample timepoints should be at least five minutes apart to allow qa _ adequate time for priming and purging the sampling lines If your system configuration includes the syringe pump and filter changer ensure the sample timepoints are at least six minutes apart Start Program Step 1 From the Ready screen press START PROG to start a program or modify a program already operating Step 2 Enter a program number between 1 and 15 and press ENTER The Link Program Option screen displays LINK PROGRAM OPTION 1 RUN SINGLE PROG 2 LINK ANOTHER PROG TIME 08 29 04 15 02 30 ELAPSE 000 00 04 Step 3 To run a single program select option 1 Note If more than ten rows are required to collect samples linking a program allows you to run tw
6. Note this option is only available if your system configuration includes the peristaltic pump 5 Prg Compl Alarm Use this option to set an alarm to sound at the end of the program It is a useful reminder that the program is complete Select option 5 Prg Compl Alarm Select option 1 to enable or option 2 to disable the alarm The Set Clock and Alarms screen displays Press any key to silence the alarm after it sounds 6 Start Output Delay Note option 6 displays only when the syringe pump is enabled If the syringe pump is enabled use this option to set the delay from the start of a program until the TTL signal is sent from the START OUTPUT jack on the VK 8000 rear panel Select option 6 Start Output Delay The Start Output Delay screen displays Enter a delay length of up to 99 59 minutes for the VK 8000 TTL output and press ENTER The Set Clock and Alarms screen displays Note this output signal can be used to start other instrumentation such as an Apparatus 3 Apparatus 7 after the program on the VK 8000 is started The default delay is 00 00 indicating that the signal will be sent at the moment a program starts For example a value of 10 00 delays the signal for ten minutes after the program starts Set the value to 00 00 to eliminate the delay feature When the syringe pump is disabled this option displays under the Main Menu See 3 Start Output Delay peristaltic pump on page 48 Var
7. Pull the paper from the front paper cutter side Pulling the paper out of the back of the printer will damage the print mechanism Unroll several inches of paper on the new roll If it is jagged cut a straight edge on the paper roll to facilitate the entry of the paper into the printer Varian Inc Page 98 VK 8000 for use with Revision D 01 06 Maintenance Apparatus 3 Apparatus 7 Operator s Manual P N 70 9039 Step 9 Slide the paper through the slot connecting the paper compartment and the printer compartment You can slide it in approximately 1 4 inch before it stops Step 10 While holding the paper in place press the printer toggle switch to the Paper feed position and hold until approximately one inch of paper has emerged from the top of the printer Caution Ensure the roll of paper feeds squarely If it does not the paper can jam and possibly damage the printer mechanism Step 11 Release the printer toggle switch Step 12 Turn the paper roll to take up any slack in the paper feeding to the printer Place the paper roll in the paper compartment Step 13 Replace the paper roll cover If the cover is difficult to remove or replace the left and right edges can be trimmed or shaved with a utility knife allowing the cover to slide easier Step 14 Toggle the printer online by pressing the printer toggle switch to the OnLine Off Line position The Ready LED illuminates Toggling Your Printer
8. an amount of sample equal to the prime loss volume flows past the valves in the VK 8000 before the valves open to collect sample The program also restricts this volume to be less than or equal to the syringe size volume Select option 3 Prime Loss Volume Enter a prime loss volume based on the sample tubing length inner diameter and system dead volumes and press ENTER The Set Syringe Pump Parameters screen displays If the tubing length between the sampling cannulas and sampling valves is changed adjust the prime loss volume accordingly 4 Aspirt Dwell Time Use this option to configure the aspiration dwell time Aspiration dwell time is the time the syringe plunger pauses at the bottom of a stroke to allow the medium being drawn to overcome the vacuum generated by the drawn plunger Select option 4 Aspirt Dwell Time Enter an aspiration dwell time between 1 and 10 seconds and press ENTER The recommended time is 10 seconds The Set Syringe Pump Parameters screen displays Varian Inc Page 56 VK 8000 for use with Revision D 01 06 Operating the VK 8000 Apparatus 3 Apparatus 7 Operator s Manual P N 70 9039 Option Function 5 Full Stroke Use this option to configure the number of steps the stepper motor hidden option makes to draw the syringe plunger its maximum stroke length Select option 5 Full Stroke Enter a full stroke value between 0 and 19999 and press ENTER The recommended value is 1
9. on page 50 Step 4 Press MENU Step 5 Select option 7 Manual Operation Step 6 Select option 4 Goto Rows Step 7 Enter 4 to move the dispensing arm to row 4 Step 8 Remove the media rinse reservoir Step 9 Press H to return to the home position Step 10 Press ESC three times to return to the Ready screen Step 11 From Ready screen press CAL The dispensing arm moves forward to allow you to check the autocalibration block Step 12 Remove the top portion of the autocalibration block and place it on its side to prevent damage Step 13 Place clean empty 10 mL calibration tubes in the bottom portion of the autocalibration block Step 14 Move the Apparatus 3 Apparatus 7 drive unit to the row which contains the tap water and begin dipping Varian Inc Page 76 VK 8000 for use with Revision D 01 06 Operating the VK 8000 Apparatus 3 Apparatus 7 Operator s Manual P N 70 9039 Step 15 Select option 1 Manual The dispensing arm moves over the autocalibration block and primes the lines according to the manual prime settings Step 16 Upon completion of the priming cycle the following screen displays PRESS lt OPEN VALVES gt TO START PRESS AGAIN WHEN 10 ML IS REACHED MANUAL CALIBRATION OF VALVE 1 TIME 08 29 04 21 56 40 ELAPSE 000 00 00 Step 17 Press OPEN VALVES The first calibration tube begins filling Step 18 Observe the first calibration tube and press OPEN VALVES again when 1
10. Apparatus 3 Apparatus 7 ENTER Enter the start date and press ENTER and start the program If the delayed start alarm is enabled enter the duration before the start of the program you would like the alarm to sound To disable the alarm see MENU gt 0 gt C under Hidden Key Functions on page 43 At any time prior to the scheduled start of the program press ESC to cancel the delayed start Varian Inc Page 70 VK 8000 for use with Revision D 01 06 Operating the VK 8000 Apparatus 3 Apparatus 7 Operator s Manual P N 70 9039 Option Start Now Function Delayed Start Function 2 Run Select option 2 Run WO Select option 2 Run WO Loading to run the VK 8000 WO Loading to start the without loading and overriding the Apparatus 3 Loading program and run the Apparatus 7 settings VK 8000 without loading Enter the start time in 24 hour format and press and overriding the ENTER Enter the start date and press ENTER oe Sp peiaey If the delayed start alarm is enabled enter the gs duration before the start of the program you would like the alarm to sound To disable the alarm see MENU gt 0 gt C under Hidden Key Functions on page 43 At any time prior to the scheduled start of the program press ESC to cancel the delayed start 3 Run Select option 3 Run Select option 3 Run VK 8000 Only to start the VK 8000 VK 8000 Only to VK 8000 only Only immediately start the Enter the start
11. Apparatus 7 The primary difference between an Apparatus 3 and an Apparatus 7 is the length of the stroke when the instrument is dipping The VK 8000 operates independent of this operating parameter The VK 8000 can store up to 15 different sampling programs Apparatus 3 Apparatus 7 parameters can be entered as part of the VK 8000 program These parameters are downloaded to the instrument when you start a run with the VK 8000 The VK 8000 fail safe sample delivery uses either the peristaltic pump see VK 8000 with Peristaltic Pump on page 16 or the VK 806 Syringe Pump optionally coupled with the automated VK 808 Filter Changer see VK 8000 with Syringe Pump on page 17 Hard copy documentation is provided by the built in Report Center Printer which prints all essential parameters for proof of proper operation and compliance with current GMPs A remote printer can also be added The VK 8000 can fill pre capped HPLC autosampler vials for direct transfer of samples to an HPLC system Septa are pierced using the exclusive needle manifold which lowers and raises at each sample timepoint Sample trays are available in a variety of sizes to accommodate either vials or test tubes Varian Inc Page 16 VK 8000 for use with Revision D 01 06 Introduction Apparatus 3 Apparatus 7 Operator s Manual P N 70 9039 The VK 8000 is automation ready An RS232 port permits integration with other automated systems Sampling runs can be act
12. Online Complete these steps to toggle your printer online Step 1 Toggle the printer online by pressing the printer toggle switch to the OnLine Off Line position When the printer is off line the Ready LED does not illuminate Step 2 Release the switch and it returns to the center position The ready LED illuminates and a READY message prints if the PRINT READY command has not been turned off See Printer Configuration on page 99 for instructions on turning on and off the PRINT READY command When you Varian Inc Revision D 01 06 VK 8000 for use with Page 99 P N 70 9039 Apparatus 3 Apparatus 7 Operator s Manual Maintenance first turn on the instrument it prints a READY message to assure you that the built in microprocessor is operating properly When you turn off the printer wait at least three seconds before turning it on again Printer Self Test You can test the print head and ribbon only after inserting paper Do not attempt to print without paper Follow these steps to perform a printer self test Step 1 Turn off the VK 8000 Step 2 Press and hold the printer toggle switch in the Paper feed position Step 3 Turn on the VK 8000 Step 4 Hold the printer toggle switch until printing begins The printer prints a list of the current configuration settings and a continuous print test Step 5 Press the printer toggle switch to the OnLine Off Line position to stop the printing operation Step 6 The
13. collected Be sure not to enter a volume larger than the collection tubes can contain 4 Prime Time peristaltic pump Select option 4 Prime Time Enter a value for the priming time and press ENTER The Manual Sampling screen displays Note the priming time is the interval the peristaltic pump runs forward drawing medium into the system before the valves open to deliver samples 4 Prime Vol syringe pump Select option 4 Prime Vol Enter a value for the priming volume and press ENTER The Manual Sampling screen displays Note the priming volume is the amount of drawn medium necessary to fill the sampling lines of the entire system 5 Purge Time peristaltic pump Select option 5 Purge Time Enter a value for the purging time and press ENTER The Manual Sampling screen displays Note the purging time is the interval the peristaltic pump runs in reverse to return the uncollected medium in each line back into the vessel from which it was drawn Purging also clears the sampling filters of particulate matter that may restrict successive samples 5 Purge Vol syringe pump Select option 5 Purge Vol Enter a value for the purging volume that ensures at least two strokes of the syringe plunger and press ENTER The Manual Sampling screen displays Note the first stroke moves all medium from the sampling lines to the return lines The second stroke draws air into the sampling lines and purges the remaining mediu
14. inch multi color Teflon lines between the remaining side of the peristaltic pump cartridges and the tubing coming from the top positions of the VK 8000 solenoid valves Ensure each cartridge has identical color tubing connected at both the inlet and the outlet Connect the tubing coming from the top positions of the VK 8000 solenoid valves to the 72 inch multi color Teflon return lines on the Apparatus 3 Apparatus 7 Ensure each line is attached to its identical color Caution Do not tighten the plastic fittings on the ends of the tubing more than half a turn past finger tight Overtightening will damage the fittings Varian Inc Chapter 5 Setting Up Your VK 8000 with Syringe Pump Cable Connections Warning The electrical connection at the back of the VK 8000 is the primary disconnect for the instrument The instrument should be positioned to allow accessibility to the power cords for easy disconnection Refer to the example diagram see Figure 2 Cable Connections for VK 8000 Syringe Pump and Apparatus 3 on page 30 to correctly make cable connections for this configuration Varian Inc Page 30 VK 8000 for use with Revision D 01 06 Setting Up Apparatus 3 Apparatus 7 Operator s Manual P N 70 9039 FIGURE 2 Cable Connections for VK 8000 Syringe Pump and Apparatus 3 APPARATUS 3 SYRINGE 5075 0252 5075 0446 Erm gt Rs485 oC Rs2s2 S
15. name address and telephone number here Please return this form via mail to Technical Writing Dissolution Systems Varian Inc 13000 Weston Parkway Cary North Carolina 27513 2250 USA Optionally you can return this form via fax at 1 919 677 1550 Always feel free to telephone us to discuss your comments at 800 229 1108 within the USA or 919 677 1108 outside the USA VK 8000 for use with Apparatus 3 Apparatus 7 Operator s Manual 70 9039 Revision D
16. page was intentionally left blank except for this message Varian Inc Chapter 3 Unpacking and Positioning Your System Check the contents for any damages due to shipping The packaging used to ship your equipment ensures that damage rarely occurs but if it does contact both the carrier who delivered the instrument and the Dissolution Systems Service Department Though claims for damage should be filed with the carrier we will be glad to help you file a claim and get you up and running as quickly as possible Varian Inc Page 20 VK 8000 for use with Revision D 01 06 Unpacking and Positioning Apparatus 3 Apparatus 7 Operator s Manual P N 70 9039 Unpacking Your VK 8000 The VK 8000 is shipped in foam filled cartons The number of cartons may vary depending on your configuration Step 1 Carefully remove the VK 8000 and accessory kit from their shipping cartons Remove all cushioning material and tape Step 2 Place the VK 8000 on aclear dry level section of the benchtop close to the Apparatus 3 Apparatus 7 The preferred placement of the VK 8000 is on the right side of the Apparatus 3 Apparatus 7 Caution Unscrew and remove the two clamps which secured the VK 8000 dispensing arm during shipping These clamps are located on both sides of the right dispensing support arm where it connects to the VK 8000 main body Failure to remove the two clamps prior to powering up the VK 8000 will damage your unit
17. power entry module Ensure the voltage indicated on the fuse drawer matches the power outlet Connect the power cord to the rear of the VK 8000 Turn on the VK 8000 The system monitor screen illuminates The initial status screen displays and then changes to the Ready screen FIGURE 4 VK 8000 Initial Status Screen VK 8000 FRACTION COLLECTOR PROGRAM REVISION x xx INITIALIZING PLEASE WAIT TIME 08 29 04 21 56 40 ELAPSE 000 00 00 The firmware version program revision displays This is the only time the firmware version displays Record the number below and refer to it if you need to call the Dissolution Systems Service Department Firmware version While the unit is powering up the dispensing arm returns to its home position at the rear of the unit and the firmware program initializes If the dispensing arm is already at the home position when the unit is powered up the initial status screen displays for three seconds only When the initial status screen disappears the Ready screen displays From the Ready screen you can access the Main Menu or any of the manual functions FIGURE 5 VK 8000 Ready Screen READY PRG 01 R M ENABLED VOLUME 5 mL TIME 08 29 04 21 56 40 ELAPSE 000 00 00 Varian Inc Revision D 01 06 VK 8000 for use with Page 41 P N 70 9039 Apparatus 3 Apparatus 7 Operator s Manual Final Setup Installing the Sample Tray Place the supplied sample tray on t
18. printer is ready to resume normal operation Printer Configuration Note Ka The printer configuration is set by the factory This procedure SE should be performed only if the printer displays erroneous characters Contact the Dissolution Systems Service Department for assistance if necessary Step 1 Turn off the VK 8000 Step 2 Press and hold the printer toggle switch in the OnLine Off Line position while turning the VK 8000 back on Hold the printer toggle switch in the Varian Inc Page 100 VK 8000 for use with Revision D 01 06 Maintenance Apparatus 3 Apparatus 7 Operator s Manual P N 70 9039 OnLine Off Line position for six seconds after the instrument is turned on then release the switch Step 3 The printer should print SETUP MENU and CONFIGURE NEXT Ok If this message does not print repeat steps 1 through 3 Step 4 The printer toggle switch is used to complete the configuration Pressing the left side of the toggle switch selects NEXT to advance to the next menu item Pressing the right side of the toggle switch selects OK to accept what is stated on the current line of the menu item Each time the switch is pressed another part of the menu prints Allow the printer to finish printing before pressing the switch again See the table of commands below gt Sakae oe The printout is easier to read if the print cover is removed SETUP MENU
19. solution Step 6 Allow the solution to remain in the syringes for ten minutes Step 7 Select option 4 Syringe Dispense The cleaning solution expels from the syringes Step 8 Replace the cleaning solution in the outer media tubes with ultrapure water Step 9 Repeat steps 3 7 a minimum of ten times to flush the syringes thoroughly Step 10 When the cleaning procedure is complete press ESC until the Ready screen displays Varian Inc Page 92 Maintenance VK 8000 for use with Revision D 01 06 Apparatus 3 Apparatus 7 Operator s Manual P N 70 9039 Acid Base Cleaning Procedure Step 1 Step 2 Step 3 Step 4 Step 5 Step 6 Step 7 Step 8 Step 9 Step 10 Step 11 Step 12 Step 13 Fill one row of outer media tubes with 0 1N NaOH Move the Apparatus 3 Apparatus 7 drive unit to the row containing the cleaning solution and begin dipping From the VK 8000 Ready screen press MENU gt 7 The Manual Operations screen displays Select option 6 Syringe Filter The Operate Syringe Filter Changer screen displays Select option 1 Valve to Input to ensure the valve is set to input Select option 3 Syringe Fill Enter 100 and press ENTER The syringes fill completely with cleaning solution Allow the solution to remain in the syringes for ten minutes Select option 4 Syringe Dispense The cleaning solution expels from the syringes Replace the cleaning solution in
20. the paper and into the printer compartment Ensure the paper is between the ribbon cartridge and the ink ribbon Ensure the ink cartridge is inserted firmly to prevent weak or irregular printing The cartridge must be properly seated and aligned for the best printing Varian Inc Revision D 01 06 VK 8000 for use with Page 97 P N 70 9039 Apparatus 3 Apparatus 7 Operator s Manual Maintenance Step 5 Turn the cartridge knob marked by an arrow clockwise to stretch the ribbon taut Step 6 Replace the cover Step 7 Toggle the printer online by pressing the printer toggle switch to the OnLine Off Line position The Ready LED illuminates Step 8 Replace the paper if necessary If you get ribbon ink on the printer s plastic cover remove it immediately Once dried it is difficult to remove Replacing a Paper Roll Step 1 Step 2 Step 3 Step 4 Step 5 Step 6 Step 7 Step 8 Toggle the printer off line by pressing the printer toggle switch to the OnLine Off Line position When the printer is off line the Ready LED does not illuminate Grasp the paper roll cover firmly by the grooves on the side and the front edge Pull outward to remove the cover Press the printer toggle switch to Paper feed to advance the paper approximately one inch beyond the paper cutter Remove the paper roll Using scissors cut the paper feeding to the printer Pull the remaining paper through the printer mechanism
21. the syringe pump is connected PUMP REV they are disabled CLEAN SYTEM Use this option to keep the valves needles and fluid lines clean See Clean System Function with Peristaltic Pump on page 82 or Clean System Function with Syringe Pump on page 86 LOCK KBRD Press LOCK KBRD to lock the keypad Press LOCK KBRD again to unlock the keypad VK 8000 Main Menu From the Ready screen press MENU The Main Menu displays Options 3 4 and 5 change depending on your system configuration When your system configuration includes the peristaltic pump the following Main Menu displays MAIN MENU 4 MANUAL PRIME TIME 1 SET CLOCK ALARMS 5 MANUAL PURGE TIME 2 SET BIO DIS 6 CONTROL BIO DIS 3 START OUTPUT DELAY 7 MANUAL OPERATION When your system configuration includes the syringe pump with or without the filter changer the following Main Menu displays MAIN MENU 4 MANUAL PRIME VOL 1 SET CLOCK ALARMS 5 MANUAL PURGE VOL 2 SET BIO DIS 6 CONTROL BIO DIS 3 SET SYRINGE PUMP 7 MANUAL OPERATION Varian Inc Page 48 Operating the VK 8000 VK 8000 for use with Revision D 01 06 Apparatus 3 Apparatus 7 Operator s Manual P N 70 9039 Following is a description of the Main Menu screen options Option Function 1 Set Clock Alarms 2 Set BIO DIS Use this option to set the clock and alarms If your system configuration includes the syringe pump the start ou
22. to any number between 1 and 99 The identification number is used when multiple units are connected in series so that the controlling or master VK 8000 can start individual units Enter a communication port identification number and press ENTER The Set BIO DIS Parameters screen displays Varian Inc Page 54 VK 8000 for use with Revision D 01 06 Operating the VK 8000 Apparatus 3 Apparatus 7 Operator s Manual P N 70 9039 Option Function 6 Print Frequency The Apparatus 3 Apparatus 7 status prints at each sample timepoint Select option 6 Print Frequency to set additional times to print the Apparatus 3 Apparatus 7 status between sample timepoints This option should be reserved for use when there is more than 30 minutes between sample timepoints Enter a print frequency and press ENTER The Set BIO DIS Parameters screen displays The print frequency timer resets after each sample timepoint Note the status does not print if the specified print frequency time falls within three minutes of the sample timepoint the sample timepoint takes precedence Once all values have been entered press ESC A confirmation screen displays Press Y to send the newly entered parameters to the Apparatus 3 Apparatus 7 and override the existing settings Main Menu Option 3 Set Syringe Pump Syringe Pump O Note Ensure the syringe pump is enabled prior to setting the syrin
23. 0 mL is reached Step 19 Repeat steps 17 and 18 for the remaining calibration tubes When the last valve is calibrated the pump reverses and the lines are purged The calibration completion alarm if enabled sounds when the calibration sequence is finished Step 20 Press any key to silence the alarm Step 21 After the dispensing arm moves forward remove and clean the calibration tubes Step 22 Reconnect the top and bottom portions of the autocalibration block Step 23 Press ESC Note If you press ESC before all the valves are calibrated you cancel a the calibration and return to the previously saved values for each valve None of the new calibration results are saved The calibration must be allowed to finish before the new calibration values are saved Varian Inc Revision D 01 06 VK 8000 for use with Page 77 P N 70 9039 Apparatus 3 Apparatus 7 Operator s Manual Operating the VK 8000 Step 24 Press MENU Step 25 Select option 7 Manual Operation Step 26 Select option 4 Goto Rows Step 27 Enter 4 to move the dispensing arm to row 4 Step 28 Replace the media rinse reservoir in front of the autocalibration block Step 29 Press H to return to the home position Step 30 Press ESC three times to return to the Ready screen Checking the Calibration Check the calibration by running a short sampling procedure and dispensing the requested volume into calibrated test tubes Step 1 Pl
24. 9300 The Set Syringe Pump Parameters screen displays If the volume dispensed by the syringe is outside of the required specifications based on a 10 mL syringe size the full stroke value can be adjusted using the following calculation and solving for x target volume mL avg volume dispensed mL x 19300 6 Pump Current hidden option Use this option to configure the pump current The use of more viscous media requires a higher pump current Select option 6 Pump Current Enter a pump current between 0 and 8 and press ENTER The recommended value is 4 The Set Syringe Pump Parameters screen displays Main Menu Option 6 Control BIO DIS From the Main Menu select option 6 Control BIO DIS The BIO DIS Control Monitor screen displays BIO DIS CONTROL MONITOR 1DIP 2STOP 3GOTOROW 4HOME SET RPM 50 ACTUAL RPM 000 0 SET BATH TEMP 37 5 ACTUAL TEMP 37 2 The following options are commands communicated from the VK 8000 to the Apparatus 3 Apparatus 7 Option Function 1 Dip Select option 1 Dip to begin dipping 2 Stop Select option 2 Stop to stop dipping and raise the cannulas Varian Inc Revision D 01 06 VK 8000 for use with Page 57 P N 70 9039 Apparatus 3 Apparatus 7 Operator s Manual Operating the VK 8000 Option Function 3 Go To Row Select option 3 Go To Row to move the drive unit to a specific row Enter a row number and press ENTER The A
25. Auto Clean displays on the Cleaning Systems Selections screen and option 2 changes to Program Auto Clean CLEANING SYSTEMS SELECTIONS 1 START CLEAN 2 PROGRAM AUTO CLEAN 3 DISABLE AUTO CLEAN TIME 08 29 04 ELAPSE 000 00 00 Note Select option 3 Disable Auto Clean to escape the automatic QE clean function and return to the original Cleaning Systems Selections screen Select option 1 Start Clean from either screen to escape the automatic clean function and immediately start the cleaning process Step 3 Select option 2 Program Auto Clean The Program Auto Clean Variables screen displays PROGRAM AUTO CLEAN VARIABLES 1 CLEAN FREQUENCY 2 DRAWING MEDIA TIME 3 PUSH FORWARD TIME TIME 08 29 04 08 28 46 ELAPSE 000 00 00 Varian Inc Revision D 01 06 VK 8000 for use with Page 85 P N 70 9039 Apparatus 3 Apparatus 7 Operator s Manual Maintenance Note c T Since the cleaning program runs concurrently with the sampling program the value entered for the clean frequency should be smaller than the sample timepoint Step 4 Select each option and enter the appropriate parameters as necessary based on tubing length tubing diameter and system dead volumes Option Function 1 Clean Frequency Select option 1 Clean Frequency Enter an interval of up to 99 minutes and press ENTER The cleaning begins at this time Note ensure the clean frequency is s
26. Electrical Hazards 10 Other 10 General 13 WEEE Directive 14 Chapter 2 Introduction 15 VK 8000 with Peristaltic Pump 16 VK 8000 with Syringe Pump 17 Conventions Used in this Manual 17 Varian Inc Table of Contents Chapter 3 Chapter 4 Chapter 5 Chapter 6 Unpacking and Positioning Your System 19 Unpacking Your VK 8000 20 Unpacking Your Peristaltic Pump 21 Unpacking Your Syringe Pump 22 Unpacking Your Filter Changer 23 Setting Up Your Sampling System 24 Setting Up Your VK 8000 with Peristaltic Pump 25 Setting Up the Peristaltic Pump 25 Cable Connections 26 Sample Tubing Connections 28 Setting Up Your VK 8000 with Syringe Pump 29 Cable Connections 29 Sample Tubing Connections 31 Setting Up Your VK 8000 with Syringe Pump and Filter Changer 33 Cable Connections 33 Sample Tubing Connections 35 Loading Filters into the Filter Changer 36 Changing Filter Types 37 Installing the Catch Basin 38 Varian Inc Table of Contents Chapter 7 Chapter 8 Final Setup 39 Power and Network Connections 39 Installing the Sample Tray 41 Installing the Media Rinse Reservoir 41 Installing and Connecting the Autocalibration Block 42 Hidden Key Functions 43 Operating the VK 8000 45 VK 8000 Keypad Options 45 VK 8000 Main Menu 47 Main Menu Option 1 Set Clock Alarms 51 Main Menu Option 2 Set BIO DIS 53 Main Menu Option 3 Set Syringe Pump Syringe Pump 54 Main Menu Option 6 Control BIO DIS 56
27. Main Menu Option 7 Manual Operation 57 Printer Operation and Communications 60 Programming the VK 8000 62 Program Option 2 Sample Time Points 66 Start Program 67 BIO DIS Options Screen 69 Manual Sample 71 Volumetric Calibration 73 Automatic Calibration 73 Manual Calibration 75 Daisy Chaining 78 Varian Inc Table of Contents Chapter 9 Chapter 10 Maintenance 81 General Maintenance 81 Clean System Function with Peristaltic Pump 82 Start Immediately 83 Programmed Clean 84 Clean System Function with Syringe Pump 86 Syringe Refurbishing 87 Removing a Leaking Syringe 87 Replacing the Seal 88 Replacing the Syringe 90 Cleaning the Syringes 91 System Cleaning Program with Syringe Pump and Filter Changer optional 93 Cleaning the Filter Changer Drip Tray 95 Report Center Impact Printer 96 Installing the Cartridge Ribbon 96 Replacing a Paper Roll 97 Toggling Your Printer Online 98 Printer Self Test 99 Printer Configuration 99 Service and Warranty 101 Exclusions and Limitations 102 Obtaining Warranty Service 102 Warranty Limitations 102 Exclusive Remedies 103 Index 105 Tell Us How We Are Doing 109 Varian Inc Figure List FIGURE 1 FIGURE 2 FIGURE 3 FIGURE 4 FIGURE 5 FIGURE 6 FIGURE 7 Cable Connections for VK 8000 Peristaltic Pump and Apparatus 7 27 Cable Connections for VK 8000 Syringe Pump and Apparatus 3 30 Cable Connections for the VK 8000 Syringe Pump Filter C
28. Page 26 VK 8000 for use with Revision D 01 06 Setting Up Apparatus 3 Apparatus 7 Operator s Manual P N 70 9039 Step 5 Push down the right side of the cartridge ensuring that the tubing remains under the cartridge and pull up the lever to lock the cartridge in place If desired trim the excess tubing to within one inch of the tubing clips _ Note gt The cover does not fit unless the cartridges are positioned with the levers on the right side Step 6 Repeat steps 3 5 for each remaining cartridge Step 7 Refit the cover and replace the two screws If the cover is not replaced correctly the pump will not operate Cable Connections Warning The electrical connection at the back of the VK 8000 is the primary disconnect for the instrument The instrument should be positioned to allow accessibility to the power cords for easy disconnection Refer to the example diagram see Figure 1 Cable Connections for VK 8000 Peristaltic Pump and Apparatus 7 on page 27 to correctly make cable connections for this configuration Varian Inc Revision D 01 06 VK 8000 for use with Page 27 P N 70 9039 Apparatus 3 Apparatus 7 Operator s Manual Setting Up FIGURE 1 Cable Connections for VK 8000 Peristaltic Pump and Apparatus 7 APPARATUS 7 VK 8000 E 5075 0042 al e e a a e n T Step 1 Connect the five pin cable 2 P N 5075 0033 bet
29. Ready screen press PROG to program the VK 8000 Step 2 Enter a program number and press ENTER The Program Variables screen displays as shown below Note The VK 8000 can store up to 15 programs in non volatile memory Valid program numbers are 1 to 15 If 0 zero or a number larger than 15 is entered the system continues to prompt you until a valid number is entered The following Program Variables screen displays when your system configuration includes the peristaltic pump PROGRAM VARIABLES 4 HEADERS 1 SET BIO DIS PARAMETERS 5 PRIME TIME 2 SAMPLE TIME POINTS 6 PURGE TIME 3 SAMPLE VOLUME 7 R M The following Program Variables screen displays when your system configuration includes the syringe pump with or without the filter changer PROGRAM VARIABLES 4 HEADERS 1 SET BIO DIS PARAMETERS 5 PRIME VOLUME 2 SAMPLE TIME POINTS 6 PURGE VOLUME 3 SAMPLE VOLUME 7 FILTER CHG or R M Varian Inc Revision D 01 06 VK 8000 for use with Page 63 P N 70 9039 Apparatus 3 Apparatus 7 Operator s Manual Operating the VK 8000 Note Option 7 displays as Filter Chg when your system configuration i includes the syringe pump and filter changer See 7 Filter Chg I syringe pump and filter changer on page 65 If your system configuration includes the syringe pump but not a filter changer option 7 displays as R M See 7 R M syringe pump on page 65 Followi
30. ace an appropriate number of 10 mL calibration tubes in row 9 of the VK 8000 sample tray Step 2 Move the Apparatus 3 Apparatus 7 drive unit to the row containing the outer media tubes and start dipping Step 3 From the Ready screen press MANUAL SAMPLE Step 4 Enter the following parameters Selection Response 2 Set Row Enter 9 and press ENTER 3 Set Volume Enter 10 and press ENTER 4 Prime Time Enter 60 and press ENTER 5 Purge Time Enter 60 and press ENTER 6 RM Option Ensure the RM option is disabled Varian Inc Page 78 VK 8000 for use with Revision D 01 06 Operating the VK 8000 Apparatus 3 Apparatus 7 Operator s Manual P N 70 9039 Step 5 Verify the values for each parameter which are shown at the bottom of the display screen for this manual program Step 6 If the values are correct select option 1 Start to start the sampling procedure The VK 8000 dispenses 10 mL into the calibration tubes in row 9 of the sample tray Step 7 Check the volume in each tube to ensure the VK 8000 is dispensing 10 mL 0 5 mL from each position Step 8 If the volumes are not correct repeat the calibration of the VK 8000 according to the procedure described on page 73 for Automatic Calibration or page 75 for Manual Calibration as appropriate If the volumes are correct you are ready to use the VK 8000 to collect samples Daisy Chaining A series of Apparatus 3 Apparatus 7
31. ave TAY VK 8000 for use with Apparatus 3 Apparatus 7 Operator s Manual P N 70 9039 January 2006 R evision Limitation of Liability The information in this document is subject to change without notice Varian Inc makes no warranty of any kind with regard to this material including but not limited to the implied warranties or merchantability and fitness for a particular purpose Varian Inc shall not be liable for errors contained herein or for incidental consequential damages in connection with the furnishing performance or use of this material All rights are reserved Reproduction adaptation or translation without prior written permission is prohibited except as allowed under copyright laws First Edition January 2006 VK 8000 for use with Apparatus 3 Apparatus 7 Operator s Manual Part Number 70 9039 Revision D Printed in the United States of America The following terms are trademarks of Varian Inc TM e Benchsaver e Practical Solutions BIO DIS III QA and QAII Enhancer Cell e TruCenter Full Flow Filters e VanKel e Peak Vessel VKE All other trademarks are the property of their respective owners Varian Inc 13000 Weston Parkway Cary North Carolina 27513 2250 800 229 1108 919 677 1108 Fax 919 677 1138 www varianinc com ap TS VARIAN Copyright c 2003 2006 by Varian Inc Table of Contents Chapter 1 Safety Practices and Hazards 9
32. clusions 102 _ exclusive remedies 103 f filter changer change filters 59 filters 36 operate 58 power connections 39 unpacking 23 final setup 39 g general maintenance 81 goto rows 58 h hazards 9 hidden key functions 43 Varian Inc Index i introduction 15 k keypad manual sample 71 options 45 start prog 67 lift valves 58 limitations 102 linking programs 68 load amp run bio dis 69 lower valves 58 m main menu 47 maintenance 81 manual calibration 75 manual operation 57 manual sample 71 77 maximum volume 63 modify program 69 o obtaining warranty service 102 online off line 98 open valves 76 open close valves 58 operating the vk 8000 45 p peristaltic pump 25 main menu 47 power connections 39 setting up 25 unpacking 21 plunger speed 55 positioning your system 19 prg compl alarm 52 prime loss volume 55 prime time 64 72 prime volume 64 72 printer cartridge ribbon 96 operation 60 replacing paper roll 97 self test 99 toggling online 98 program variables screen 62 purge time 64 72 purge volume 65 72 push forward time 85 r ready screen 40 replacement media rm option 72 replacing paper roll 97 report center printer operation 60 row compl alarm 51 rs232 30 run vk 8000 only 70 run wo loading 70 Ss safety practices 9 sample timepoints 66 sample times 61 sample tray 41 sample tubing connections 28 31 35 screens initial status 40 ready 40 syst
33. d Options The keypad on the VK 8000 is similar to a personal computer keyboard For example it has a SPACE key an ENTER key and a SHIFT key These keys function exactly like their counterparts on a personal computer or typewriter keyboard See Figure 6 VK 8000 Keypad below A discussion about each of the main function keys follows FIGURE 6 VK 8000 Keypad SYSTEM MONITOR w a ee amp Bo i a Gee ae a a aE 3 E BACK SPACE o w R T Y U i fs pP SPACE RG fo A s 0 F a H J x L ENTER SHIFT Ze ie oe Ave Gee oa oa ma m S Varian Inc Page 46 VK 8000 for use with Revision D 01 06 Operating the VK 8000 Apparatus 3 Apparatus 7 Operator s Manual P N 70 9039 The front panel options include the following Option Function ESC Press ESC to stop execution of a running program or calibration routine Press ESC to move back one level to the previous menu or selection when moving through the multi level menu Be very careful when using ESC while a program is running If a program or calibration is running and ESC is pressed a prompt appears on the first line of the display asking if you want to stop the running program The options are Y or N Press Y to stop the running program Press N to continue the running program MENU Use this option to set the clock alarms Apparatus 3 Apparatus 7 parameters start output delay syringe pump parameters manual prime time volume manual purge time volume as wel
34. disabled or the instrument will not operate properly Varian Inc Page 66 VK 8000 for use with Revision D 01 06 Operating the VK 8000 Apparatus 3 Apparatus 7 Operator s Manual P N 70 9039 Program Option 2 Sample Time Points From the Program Variables screen select option 2 Sample Time Points The following screen displays SAMPLE TIME POINT 000 02 ENTER hhh mm UP TO 999 59 HOURS PROG 1 ROW 0 VOLUME 5ml TIME 08 29 04 09 37 14 ELAPSE 000 00 00 Note This screen accepts sampling times and saves them for execution when the program is run Timepoints are requested in order of S execution starting with collection row number 0 on the sample tray The sample time is entered as hours and minutes Once you enter a time the row number increments and another sample timepoint is requested The following conventions are used with this screen e Review previously entered sample timepoints by pressing ENTER to scroll through the entries e After all desired sample timepoints have been entered enter 000 00 for the last sample timepoint and press ENTER If the maximum of ten sample timepoints are entered you do not have to enter 000 00 for the last sample timepoint e To add additional sample timepoints press ENTER until a new row time displays Enter the additional sample timepoint e f more than ten sample timepoints are needed use the link program feature See Start Program
35. e Prior to beginning the clean system function verify an aqd z gt appropriate manual prime value and purge value have been entered via the Main Menu See 4 Manual Prime Time peristaltic pump on page 49 and 5 Manual Purge Time peristaltic pump on page 50 To clean the system complete the following steps Step 1 Place the appropriate number of outer media tubes in row 1 of the Apparatus 3 Apparatus 7 Step 2 Fill each outer media tube with an appropriate cleaning solution usually water Step 3 From the Main Menu select option 6 Control BIO DIS See Main Menu Option 6 Control BIO DIS on page 56 Step 4 Select option 3 Goto Row Step 5 Enter 1 and press ENTER to move the drive unit to row 1 which contains the outer media tubes filled with cleaning solution Step 6 Select option 1 Dip to lower the agitator shafts and begin dipping A slow dipping speed such as 5 DPM is recommended See 1 Dipping Speed DPM on page 53 for information about how to set the dipping speed Varian Inc Revision D 01 06 VK 8000 for use with Page 83 P N 70 9039 Apparatus 3 Apparatus 7 Operator s Manual Maintenance Step 7 Once dipping has started press CLEAN SYSTEM on the VK 8000 The Cleaning Systems Selections screen displays CLEANING SYSTEMS SELECTIONS 1 START CLEAN 2 ENABLE AUTO CLEAN TIME 08 29 04 08 28 46 ELAPSE 000 00 00 To start the cleanin
36. e Replacement Media Option screen displays Select option 2 Disable The Program Variables screen displays Note the replacement media option is not available for use with the Apparatus 3 Apparatus 7 The replacement media option must be disabled or the instrument will not operate properly 7 Filter Chg syringe pump and filter changer If your system configuration includes the syringe pump and filter changer select option 7 Filter Chg Enter the desired number of samples each filter should process before being discharged and press ENTER The recommended value is 1 The Program Variables screen displays Note if your system configuration includes the syringe pump and filter changer ensure the syringe pump option is enabled From the Ready screen press MENU Select option 3 Set Syringe Pump Select option 1 Enable Also press MENU gt 0 gt P and select option 1 Enable The replacement media option is not available with this system configuration Ensure the RM option for the syringe pump is disabled using the hidden key function MENU gt 0 gt R 7 R M syringe pump If your system configuration includes the syringe pump but not the filter changer select option 7 R M The Replacement Media Option screen displays Select option 2 Disable The Program Variables screen displays Note the replacement media option is not available for use with the Apparatus 3 Apparatus 7 The replacement media option must be
37. e TUVus mark signifies that these products have been tested to U S standards and certified for the U S market The cTUV mark signifies that these products have been tested to Canadian standards and certified for the Canadian market When the two marks are coupled the cTUVus mark signifies that these products have been tested to standards and certified for both markets Varian Inc Page 14 VK 8000 for use with Revision D 01 06 Safety Practices and Hazards Apparatus 3 Apparatus 7 Operator s Manual P N 70 9039 WEEE Directive All Varian products that are subject to the WEEE directive shipped after August 13 2005 are compliant with the WEEE marking requirements Such products are marked with the crossed out wheelie bin WEEE symbol shown on page 12 in accordance with European Standard EN 50419 This symbol on the product or on its packaging indicates that this product must not be disposed of as unsorted municipal waste The separate collection and recycling of your waste equipment at the time of disposal will help to conserve natural resources and ensure that it is recycled in a manner that protects human health and the environment For more information on collection reuse and recycling systems please contact your local regional waste administration your local distributor or Varian Inc Varian Inc chapter2 Introduction The VK 8000 Dissolution Sampling Station is designed to work with USP Apparatus 3 and USP
38. e waste container If your system configuration includes the syringe pump press H to move the dispensing arm to the home position and press ESC three times to return to the Ready screen If you system configuration includes the peristaltic pump complete the steps under Installing and Connecting the Autocalibration Block below Installing and Connecting the Autocalibration Block Note Q lt The autocalibration unit is available for use only if your system configuration includes the peristaltic pump Step 1 Step 2 Step 3 Step 4 With the dispensing arm forward insert the autocalibration block behind the media rinse reservoir Ensure the RS232 port is positioned to the right for a 6 row configuration and toward the back for a 12 row configuration Connect the nine pin RS232 cable between the CALIBRATION port on the VK 8000 rear panel and the RS232 port on the side of the autocalibration block Press H to move the dispensing arm to the home position Press ESC three times to return to the Ready screen Varian Inc Revision D 01 06 VK 8000 for use with Page 43 P N 70 9039 Apparatus 3 Apparatus 7 Operator s Manual Final Setup Hidden Key Functions Key Sequence Function MENU gt 0 gt 0 Use this key sequence to select the system configuration Select the option that corresponds to the number of valves on your VK 8000 MENU gt 0 gt 8 Use this key sequence to select a f
39. eady screen press MENU The Main Menu displays Step 3 Select option 7 Manual Operation The Manual Operations screen displays Step 4 Select option 6 Syringe Filter Step 5 From the Operate Syringe Filter Changer screen select option 5 Filter Changer The Filter Changer Manual Control screen displays Step 6 Select option 3 Change Filters Step 7 Enter 8 as the number of filters to change and press ENTER The filters expel from the filter changer Step 8 Repeat steps 6 and 7 until filters no longer expel from the filter changer The following error message displays on the screen FILTER CHANGER ROTOR ERROR THE FILTER CHANGER IS EMPTY OR HAS FAILED TO LOAD PROPERLY PRESS lt 1 gt TO CONTINUE OR lt ESC gt TO QUIT Step 9 Press ESC The Filter Changer Manual Control screen displays Step 10 Load the new tubes of filters into the carousel of the filter changer See Loading Filters into the Filter Changer on page 36 Step 11 Select option 3 Change Filters Step 12 Enter 8 as the number of filters to change and press ENTER The filters expel from the filter changer Varian Inc Page 38 VK 8000 for use with Revision D 01 06 Setting Up Apparatus 3 Apparatus 7 Operator s Manual P N 70 9039 Step 13 When the filter changer stops expelling filters select option 3 Change Filters again Step 14 Enter 1 as the number of filters to change and press ENTER The remaining filter of the previous
40. ect option 4 Goto Rows to move the VK 8000 dispensing arm to a specific row Enter a row number The dispensing arm moves to the indicated row and stops If the moving alert is enabled the alarm sounds when the dispensing arm moves To disable the alert see MENU gt 0 gt B under Hidden Key Functions on page 43 5 Turn On Off Pump R M Ensure TURN ON R M PUMP displays as option 5 This indicates the replacement media option is disabled If TURN OFF R M PUMP displays select option 5 TURN ON R M PUMP displays Note the replacement media option must be disabled or the instrument will not operate properly 6 Syringe Filter syringe pump only changer as applicable See Manual Operation Option 6 Use this option to manually control the syringe pump and filter Syringe Filter below Manual Operation Option 6 Syringe Filter From the Manual Operations screen select option 6 Syringe Filter The Operate Syringe Filter Changer screen displays OPERATE SYRINGE FILTER CHANGER 1 VALVE TO INPUT 2 VALVE TO OUTPUT 3 SYRINGE FILL 4 SYRINGE DISPENSE 5 FILTER CHANGER 6 SYRINGE PARAMETERS Varian Inc Revision D 01 06 P N 70 9039 VK 8000 for use with Page 59 Apparatus 3 Apparatus 7 Operator s Manual Operating the VK 8000 Following is a description of the Operate Syringe Filter Changer screen options Option Function 1 Valve to Input The syringe pu
41. em monitor 40 service 101 set alarms 51 set baud rate 61 set bio dis 53 set clock 51 set com portid 61 set dipping speed 53 set row number 71 set syringe pump 54 set volume 72 setting up 24 start clean 83 start mode 68 start program 67 syringe pump enabling 44 fill syringe 59 operate 58 power connections 39 set syringe size 55 setting 54 syringe dispense 59 syringe parameters 55 unpacking 22 t teflon lines 35 36 tie wraps 28 31 35 timer alarm 51 Varian Inc Index u unpacking 19 vV valve to input 58 valve to output 58 vk 8000 clamp removal 20 unpacking 20 volumetric calibration 73 w warranty 101 warranty limitations 102 Varian Inc Index This page was intentionally left blank except for this message Varian Inc ap m8 VARIAN Tell Us How We Are Doing We listen to our customers We work hard to make our technical documentation user friendly and to make the information in our manuals easy to retrieve and use We d like you to tell us the kinds of additional information you d find helpful in our documentation Your feedback will be carefully considered when we prepare future editions of this manual This manual should contain the following additional information The most useful thing about this book is This manual would be more helpful if My general impressions of this book are May we contact you regarding your comments YES NO If yes please write your
42. ess ESC until the Ready screen displays Step 8 Press START PROG Step 9 Enter the program number for this cleaning program and press ENTER Step 10 Press 1 gt 1 gt 1 to start the cleaning program Varian Inc Revision D 01 06 VK 8000 for use with Page 95 P N 70 9039 Apparatus 3 Apparatus 7 Operator s Manual Maintenance After the VK 8000 pulls three rows of samples for cleaning purposes complete the following steps to ensure the needles are completely purged of cleaning solution Step 1 From the Ready screen press MENU gt 7 gt 4 gt R Step 2 Press ESC The Manual Operations screen displays Step 3 Select option 3 Open Close Valves to open the valves Step 4 Select option 2 Lower Valves to lower the needles Step 5 Select option 1 Lift Valves to raise the needles Step 6 Repeat steps 4 and 5 a minimum of four times to completely expel the cleaning solution from the needles Step 7 Select option 3 Open Close Valves to close the valves Step 8 Select option 4 Goto Rows Step 9 Press H to move the dispensing arm to the home position Step 10 Press ESC until the Ready screen displays Cleaning the Filter Changer Drip Tray Step 1 Slide up and remove the filter catch basin from the screw mount on the front of the filter changer Step 2 Pull the drip tray forward completely to remove it from under the filter changer Step 3 Empty any accumulated liquid from the tray and wipe it clean
43. et earlier than the first sample timepoint 2 Drawing Media Time Select option 2 Drawing Media Time Enter an interval of up to 999 seconds to control the length of time the cleaning solution is drawn from the rinse tank and press ENTER 3 Push Forward Time Select option 3 Push Forward Time Enter an interval of up to 999 seconds to indicate the length of time the cleaning solution is moved through the return lines and press ENTER Note Appropriate parameters for each of the above options depends on the length and diameter of the tubing as well as the needle volume Do not allow the cleaning solution to reach the Apparatus 3 Apparatus 7 Once the pump reverses the cleaning solution is expelled in the rinse chamber At the time set for the clean frequency see 1 Clean Frequency above the VK 8000 drive unit moves to the rinse chamber and lowers the needles The peristaltic pump draws the cleaning solution into the needles and sample lines for the length of time defined as the drawing media time see 2 Drawing Media Time above The valves close and the pump pushes the cleaning solution into the return lines for the programmed push forward time see 3 Push Forward Time above Ensure the length of time entered for the push forward time does not push the cleaning solution far enough to reach the Apparatus 3 Apparatus 7 or sample contamination can occur The peristaltic pump reverses and t
44. fic legal rights which may vary from state to state or province to province so you may have other rights and some of these exclusions may not apply to you Varian Inc Page 103 Revision D 01 06 VK 8000 for use with Service and Warranty P N 70 9039 Apparatus 3 Apparatus 7 Operator s Manual Exclusive Remedies The remedies provided herein are the customer s sole and exclusive remedies In no event shall Varian Inc or its representatives be liable for any direct indirect special incidental or consequential damages whether based on contract tort or any other legal theory Some states or provinces do not allow the exclusion or limitation of incidental or consequential damages so the above limitation or exclusion may not apply to you Varian Inc Page 104 VK 8000 for use with Revision D 01 06 Service and Warranty Apparatus 3 Apparatus 7 Operator s Manual P N 70 9039 This page was intentionally left blank except for this message Varian Inc Index a aspirt dwell time 55 autocalibration block 42 automatic calibration 73 b batch information 61 c cable connections 26 29 33 calib compl alarm 52 calibration checking 77 change filters 37 clean frequency 85 clean system 82 com port id 53 connections cable 26 29 33 power and network 39 control bio dis 56 conventions 17 d dipping speed 53 drawing media time 85 drop volume 43 dual voltage option 40 e ex
45. g process immediately see Start Immediately below To program the auto clean function see Programmed Clean on page 84 Start Immediately From the Cleaning Systems Selections screen select option 1 Start Clean The VK 8000 immediately starts to clean the system by priming the lines Once the system completes the priming the needle manifold jogs up and down while opening and closing the valves This motion rinses the needles in the VK 8000 rinse chamber Then the system purges the cleaning solution from the sample lines The following figure is a generalized flow diagram of the clean cycle see Figure 7 The Cleaning Process below You should use this function often FIGURE 7 The Cleaning Process Place Probes Move i A Open Close in Rinse Prime Tubing Head to Rinse Valves Solution Position Reverse Pump Purge Tubing Clear Filters Varian Inc Page 84 VK 8000 for use with Revision D 01 06 Maintenance Apparatus 3 Apparatus 7 Operator s Manual P N 70 9039 Programmed Clean To run a program concurrently with the sampling program which rinses the needles in a predetermined cleaning solution contained in the rinse chamber complete the following steps Step 1 Ensure the rinse chamber of the media rinse reservoir is filled with the cleaning solution Step 2 From the Cleaning Systems Selections screen select option 2 Enable Auto Clean Option 3 Disable
46. ge pump parameters see MENU gt 0 gt P under Hidden Key Functions on page 44 From the Main Menu select option 3 Set Syringe Pump Select option 2 to disable the syringe pump or select option 1 to enable the syringe pump Once enabled the Set Syringe Pump Parameters screen displays 1 SYRINGE SIZE 2 PLUNGER SPEED 3 PRIME LOSS VOLUME 4 ASPIRT DWELL TIME SET SYRINGE PUMP PARAMETERS Varian Inc Revision D 01 06 VK 8000 for use with Page 55 P N 70 9039 Apparatus 3 Apparatus 7 Operator s Manual Operating the VK 8000 Following is a description of the Set Syringe Pump Parameters screen options Option Function 1 Syringe Size Use this option to set the syringe size Select option 1 Syringe Size Select the syringe size that corresponds with the Kloehn syringes loaded into the syringe pump The Set Syringe Pump Parameters screen displays 2 Plunger Speed Use this option to configure the syringe pump plunger to operate ata specific speed Select option 2 Plunger Speed Enter a plunger speed between 100 and 1500 motor steps per second sps and press ENTER The recommended value is 500 The Set Syringe Pump Parameters screen displays 3 Prime Loss Use this option to configure the prime loss volume Prime loss Volume volume compensates for the volume of medium used to fill the tubing between the sampling cannulas and sampling valves during the priming cycle When sampling
47. guration includes the peristaltic pump the following Manual Sampling screen displays MANUAL SAMPLING 1 START 2 SET ROW 3 SET VOLUME 4 PRIME TIME 5 PURGE TIME 6 R M OPTION ROW3 VOLUME 5m PRIME 60 PURGE 60 When your system configuration includes the syringe pump with or without the filter changer the following Manual Sampling screen displays MANUAL SAMPLING 1 START 2 SET ROW 3 SET VOLUME 4 PRIME VOL 5 PURGE VOL 6 R M OPTION ROW3 VOLUME 5m PRIME 10 PURGE 20 Following is a description of the Manual Sampling screen options Option Function 1 Start Select option 1 Start to start the manual sampling procedure using the values you enter for options 2 through 6 on the Manual Sampling screen The display screen details each step of the manual collection as it takes place Note select this option last 2 Set Row Select option 2 Set Row Enter the row number between 0 and 9 where you want the samples deposited in the sample tray and press ENTER The Manual Sampling screen displays Varian Inc Page 72 VK 8000 for use with Revision D 01 06 Operating the VK 8000 Apparatus 3 Apparatus 7 Operator s Manual P N 70 9039 Option Function 3 Set Volume Select option 3 Set Volume Enter a sample volume between 0 and 14 mL and press ENTER The Manual Sampling screen displays Note the volume can contain a decimal point so that fractional milliliters can be
48. h have their own individual priming volume values It is used only with the MANUAL SAMPLE and CLEAN SYSTEM keys on the VK 8000 Varian Inc Page 50 VK 8000 for use with Revision D 01 06 Operating the VK 8000 Apparatus 3 Apparatus 7 Operator s Manual P N 70 9039 Option Function 5 Manual Purge Time peristaltic pump 5 Manual Purge Vol syringe pump If your system configuration includes the peristaltic pump use this option to set the interval the peristaltic pump runs in reverse to return the uncollected medium in each line back into the vessel from which it was drawn Select option 5 Manual Purge Time Enter a value for the manual purge time from 1 to 99 seconds and press ENTER The Main Menu displays Note purging also clears the sampling filters of particulate matter that may restrict successive samples As with priming the longer the tubing lengths the longer the purge time required This value is not used by sampling programs which have their own individual purging time values It is used only with the MANUAL SAMPLE CAL and CLEAN SYSTEM keys on the VK 8000 If your system configuration includes the syringe pump use this option to set the a purge volume that ensures all stranded medium is properly expelled Select option 5 Manual Purge Vol Enter a value for the purge volume that ensures at least two strokes of the plunger and press ENTER The Main Menu displays Note the first stroke move
49. hanger and Apparatus 3 34 VK 8000 Initial Status Screen 40 VK 8000 Ready Screen 40 VK 8000 Keypad 45 The Cleaning Process 83 Varian Inc Figure List This page was intentionally left blank except for this message Varian Inc chapter1 Safety Practices and Hazards The VK 8000 and accessories have been carefully designed so that when used properly you have an accurate fast flexible and safe instrument If the equipment is used in a manner not specified by the manufacturer the protection provided by the equipment may be impaired Operation of a VK 8000 and accessories involves the use of aqueous liquids Unskilled improper or careless use of this instrument can create shock hazards fire hazards or other hazards which can cause death serious injury to personnel or severe damage to equipment and property Information on safety practices is provided with your instrument and operation manuals Before using your instrument or accessories you must thoroughly read these safety practices Observe all relevant safety practices at all times Varian Inc Page 10 VK 8000 for use with Revision D 01 06 Safety Practices and Hazards Apparatus 3 Apparatus 7 Operator s Manual P N 70 9039 Electrical Hazards The VK 8000 and accessories contain electrical circuits devices and components operating at dangerous voltages Contact with these circuits devices and components can cause death serious inju
50. he cleaning solution is pulled back through the sample lines The pump Varian Inc Page 86 VK 8000 for use with Revision D 01 06 Maintenance Apparatus 3 Apparatus 7 Operator s Manual P N 70 9039 reverses again the valves open and the cleaning solution is expelled into the rinse chamber The VK 8000 activates the valves several times to clean the valves and needles Clean System Function with Syringe Pump To clean the system immerse all sampling cannulas into a cleaning solution usually water Complete the following steps Step 1 Fill one row of outer media tubes with the appropriate cleaning solution usually water Step 2 On the Apparatus 3 Apparatus 7 press START DIP Step 3 Select option 3 Goto Row Step 4 Enter the row number which contains the cleaning solution and press ENTER The drive unit moves to the selected row Step 5 Select option 2 Set Speed DPM Step 6 Enter 5 DPM and press ENTER Step 7 Select option 1 Start The agitator shafts lower and begin dipping Step 8 On the VK 8000 press CLEAN SYSTEM The Cleaning Systems Selections screen displays and the cleaning procedure begins immediately Upon completion of the cleaning process the VK 8000 dispensing arm and the Apparatus 3 Apparatus 7 drive unit return to the home position Varian Inc Revision D 01 06 VK 8000 for use with Page 87 P N 70 9039 Apparatus 3 Apparatus 7 Operator s Manual Maintenance Syr
51. ian Inc Revision D 01 06 VK 8000 for use with Page 53 P N 70 9039 Apparatus 3 Apparatus 7 Operator s Manual Operating the VK 8000 Main Menu Option 2 Set BIO DIS From the Main Menu select option 2 Set BIO DIS Enter a program number and press ENTER The Set BIO DIS Parameters screen displays SET BIO DIS PARAMETERS 1 DIPPING SPEED DPM 2 SAMPLES PER ROW 3 HOLD DIP TIME 4 SET BATH TEMP 5 COM PORT ID 6 PRINT FREQUENCY Following is a description of the Set BIO DIS Parameters screen options Option Function 1 Dipping Speed Select option 1 Dipping Speed DPM Enter the desired DPM DPM and press ENTER The Set BIO DIS Parameters screen displays 2 Samples Per Row Select option 2 Samples Per Row Enter the desired number of samples to be taken from each row and press ENTER The Set BIO DIS Parameters screen displays 3 Hold Dip Time Select option 3 Hold Dip Time Enter a hold dip time and press ENTER to advance to each remaining row After a hold dip time is entered for the final row the Set BIO DIS Parameters screen displays 4 Set Bath Temp Select option 4 Set Bath Temp Enter the desired water bath temperature and press ENTER The Set BIO DIS Parameters screen displays 5 Com Port ID Select option 5 Com Port ID to change the communication port identification number Your system was shipped with the communication port identification number set to 01 You can set the identification number
52. ime is incorrect enter the correct time in 24 hour format and press ENTER The Set Clock and Alarms screen displays 2 Timer Alarm Use this option to set the elapsed timer alarm The elapsed timer alarm is a simple timer you can use to time any interval from 1 minute to 99 hours The alarm sounds when the time has expired Once turned off the timer does not operate again until you reset it Select option 2 Timer Alarm Enter a duration in hh mm format and press ENTER The Set Clock and Alarms screen displays Press any key to silence the alarm after it sounds 3 Row Compl Alarm Use this option to set an alarm to indicate the completion of each sample timepoint This alarm is useful when the samples must be checked or removed for immediate analysis Select option 3 Row Compl Alarm Select option 1 to enable or option 2 to disable the alarm The Set Clock and Alarms screen displays Press any key to silence the alarm after it sounds Varian Inc Page 52 VK 8000 for use with Revision D 01 06 Operating the VK 8000 Apparatus 3 Apparatus 7 Operator s Manual P N 70 9039 Option Function 4 Calib Comp Alarm Use this option to set an alarm to sound when autocalibration is complete and all lines are purged Select option 4 Calib Compl Alarm Select option 1 to enable or option 2 to disable the alarm The Set Clock and Alarms screen displays Press any key to silence the alarm after it sounds
53. inge Refurbishing The following instructions are for the Syringe Refurbishing Kit P N 15 5900 for use with Luer syringes P N 1003 0145 The refurbishing kit contains e white seal e O ring Note qi s Ensure the white seal remains clean Keep the seal free from dirt and dust Removing a Leaking Syringe Complete the following instructions to remove the leaking syringe from the syringe pump Step 1 Lay the syringe pump on its back Step 2 From the VK 8000 Ready screen press MENU gt 7 to display the Manual Operations screen Step 3 Select option 6 Syringe Filter The Operate Syringe Filter Changer screen displays Step 4 Select option 3 Syringe Fill Step 5 Enter 100 and press ENTER The syringe plungers move to their lowest position The menu remains on the VK 8000 display screen for later use Step 6 Unscrew the locking screw at the base of the leaking syringe Step 7 Remove the screw and gently remove the plunger from the syringe barrel Varian Inc Page 88 VK 8000 for use with Revision D 01 06 Maintenance Apparatus 3 Apparatus 7 Operator s Manual P N 70 9039 Step 8 Unscrew the syringe barrel by turning the metal fitting on top of the syringe barrel counterclockwise until the syringe comes free Replacing the Seal Note Ue The seal and O ring can be replaced up to ten times before the glass syringe needs to be replaced Step 1 If necessa
54. ivated with an external switch closure or TTL signal The VK 8000 has a programmable TTL output to control the instruments in the system Warning The VK 8000 and accessories contain electrical circuits devices and components operating at dangerous voltages Contact with these circuits devices and components can cause death serious injury or painful electric shock Caution Panels or covers that are retained by fasteners which require the use of a tool for removal may be opened only by Varian trained Varian qualified or Varian authorized service engineers VK 8000 with Peristaltic Pump The VK 8000 when used in conjunction with the peristaltic pump is capable of performing unattended sampling from an Apparatus 3 Apparatus 7 All parameters of operation are controlled by the VK 8000 Sampling can be carried out automatically and takes into account all relevant variables in the sample flow path such as tubing diameter length and tension Samples may be delivered into open test tubes or pre capped autosampler vials Note q amp a It is necessary to use the peristaltic pump with the 12 position 12 row Apparatus 7 This configuration does not work with the syringe pump Varian Inc Revision D 01 06 VK 8000 for use with Page 17 P N 70 9039 Apparatus 3 Apparatus 7 Operator s Manual Introduction VK 8000 with Syringe Pump The VK 8000 Dissolution Sampling Station when used in con
55. ixed 1200 baud ora programmable baud Select option 2 Programmable Baud Note if you are using the VK 8000 with other Varian equipment the baud rate must be set to 9600 baud see Printer Operation and Communications on page 60 MENU gt 0 gt A Use this key sequence to set the drop volume before sampling Enter the desired drop volume and press ENTER The maximum acceptable value is 1 Note the drop volume is an amount of sample that is dispensed through the VK 8000 needles prior to dispensing samples into the collection tubes to ensure the needles are purged completely MENU gt 0 gt B Use this key sequence to enable or disable the moving alert The asterisk displays next to the current selection If enabled the alarm sounds when the dispensing arm is in motion MENU gt 0 gt C Use this key sequence to enable or disable the delayed start alarm The asterisk displays next to the current selection If the delayed start alarm is enabled the alarm sounds prior to the start of the program See BIO DIS Options Screen on page 69 MENU gt 0 gt D Use this key sequence to enable or disable the multiple sample option The asterisk displays next to the current selection Note this option must be enabled to pull multiple samples from each row see 2 Samples Per Row on page 53 MENU gt 0 gt E Use this key sequence to select the printer type Ensure the asterisk displays next to option 1 I
56. junction with the VK 806 Syringe Pump with or without the VK 808 Filter Changer is capable of performing unattended sampling from an Apparatus 3 Apparatus 7 All parameters of operation are controlled by the VK 8000 Variables in the flow path such as tubing diameter and length are accounted for in the sampling process Samples may be delivered into open test tubes or pre capped autosampler vials The syringe pump incorporates the use of up to eight individual syringes housed in a single unit The syringe pump provides syringe driven accuracy of sampling Introducing the filter changer into the system provides superior removal of particulates through the use of membrane filters Conventions Used in this Manual e ltems you are asked to press are in bold For example press H on the keypaa e Key sequences you are asked to press appear like this MENU gt 4 Note Remember to return the warranty card supplied with this manual Completing and returning the card ensures your right to q a protection under the terms and conditions of your warranty It also enables us to better assist you in the event of any problems Additionally it guarantees you will be informed of any issues that arise concerning your equipment such as upgrades retrofits or regulatory changes Varian Inc Page 18 VK 8000 for use with Revision D 01 06 Introduction Apparatus 3 Apparatus 7 Operator s Manual P N 70 9039 This
57. l as to remotely control the Apparatus 3 Apparatus 7 and manually control the VK 8000 See VK 8000 Main Menu on page 47 PRINT Press PRINT to record the batch information print the dip and drain times enable or disable the Report Center Printer select a local or remote printer set the communication port identification number and set the baud rate See Printer Operation and Communications on page 60 CAL Use this option to calibrate the VK 8000 to ensure that sample volumes are accurately and precisely taken See Volumetric Calibration on page 73 PROG Use this option to set program parameters See Programming the VK 8000 on page 62 START PROG Use this option to start a program See Start Program on page 67 MANUAL SAMPLE Use this option to collect a sample from the dissolution vessels on command without a program See Manual Sample on page 71 OPEN VALVES Press OPEN VALVES to open all valves simultaneously This function keeps the valves open as long as the key on the keypad is pressed To change the valve configuration press MENU gt 0 gt 0 and select the appropriate configuration Varian Inc Revision D 01 06 VK 8000 for use with Page 47 P N 70 9039 Apparatus 3 Apparatus 7 Operator s Manual Operating the VK 8000 Option Function PUMP OFF PUMP OFF PUMP FWD and PUMP REV work exclusively with PUMP FWD the Varian peristaltic pump When
58. lso placed alongside warnings Varian Inc Revision D 01 06 VK 8000 for use with Page 11 P N 70 9039 Apparatus 3 Apparatus 7 Operator s Manual Safety Practices and Hazards These symbols are also used on warning labels attached to the instrument When you see one these symbols you must refer to the relevant operation or service manual for the correct procedure referred to by that warning label The meaning of the symbols that appear alongside warnings in this manual are as follows Electrical shock Pinch point Caution Refer to accompanying documents Read all warnings and cautions carefully and observe them at all times Varian Inc Page 12 VK 8000 for use with Revision D 01 06 Safety Practices and Hazards Apparatus 3 Apparatus 7 Operator s Manual P N 70 9039 Caution A Caution message appears in the manual when failure to observe instructions could result in damage to equipment Varian supplied and or other associated equipment A Note appears in the manual to give advice or information ux Information Symbols Switches main power on Switches main power off Indicates single phase alternating current Indicates the product complies with the requirements of one or C more European Union EU directives Indicates specific equipment meets consensus based standards IN of safety to provide assurance required by OSHA that these af se products are safe for use i
59. m from the return lines This ensures all stranded medium is properly expelled 6 R M Option Select option 6 R M Option The Replacement Media Option screen displays Select option 2 to disable media replacement The Manual Sampling screen displays Note the media replacement option is not available with the Apparatus 3 Apparatus 7 Ensure this option is disabled or the instrument will not operate properly Varian Inc Revision D 01 06 VK 8000 for use with Page 73 P N 70 9039 Apparatus 3 Apparatus 7 Operator s Manual Operating the VK 8000 Volumetric Calibration Note The CAL key on the VK 8000 can be used only if the peristaltic QS pump is installed on your system To calibrate the syringes in the VK 806 Syringe Pump see 5 Full Stroke hidden option under Main Menu Option 3 Set Syringe Pump Syringe Pump on page 56 It is critical that sample volumes are accurately and precisely taken Calibration of your system ensures this by recording in memory the time required to deliver a nominal 10 mL of medium into the graduated calibration tubes Refer to Automatic Calibration below or Manual Calibration on page 75 for specific operating procedures necessary to calibrate your VK 8000 Note Ensure the cannula filters are in place during calibration Automatic Calibration To perform automatic calibration complete the following steps Step 1 Place 300
60. mL outer media tubes filled with tap water in a row on the Apparatus 3 Apparatus 7 Step 2 Ensure the correct prime and purge times have been entered via the Main Menu options 4 and 5 Manual Prime Time and Manual Purge Time respectively see 4 Manual Prime Time peristaltic pump on page 49 and 5 Manual Purge Time peristaltic pump on page 50 Step 3 Ensure the supplied autocalibration block is firmly in place and connected to the rear panel jack labeled AUTO CALIBRATION Step 4 From the Ready screen press CAL The dispensing arm moves forward to allow you to check the autocalibration block Varian Inc Page 74 VK 8000 for use with Revision D 01 06 Operating the VK 8000 Apparatus 3 Apparatus 7 Operator s Manual P N 70 9039 Step 5 Step 6 Move the Apparatus 3 Apparatus 7 drive unit to the row which contains the tap water and begin dipping The VK 8000 system monitor displays a reminder to ensure the tubing and pump are ready The Calibrate Sampling Volume screen displays CALIBRATE SAMPLING VOLUME CHECK PUMP amp TUBING IF AUTO CALIBRATION INSTALL CALIB FIXTURE BEFORE STARTING 1 MANUAL 2 AUTOMATIC Select option 2 Automatic The dispensing arm moves over the autocalibration block and primes the lines according to the manual prime settings At the end of the priming cycle all valves lower open simultaneously and begin to fill the autocalibration block As the 10 mL
61. mp Run BIO DIS The program starts Each unit starts running the program you entered Note Ensure no start output delays have been set on any of the VK 8000s See 3 Start Output Delay peristaltic pump under VK 8000 Main Menu on page 48 or 6 Start Output Delay under Main Menu Option 1 Set Clock Alarms on page 52 depending on your system configuration Varian Inc Page 80 VK 8000 for use with Revision D 01 06 Operating the VK 8000 Apparatus 3 Apparatus 7 Operator s Manual P N 70 9039 This page was intentionally left blank except for this message Varian Inc Chapter 9 Maintenance General Maintenance Warning The VK 8000 and accessories contain electrical circuits devices and components operating at dangerous voltages Contact with these circuits devices and components can cause death serious injury or painful electric shock Perform the following maintenance when necessary Varian Inc Page 82 VK 8000 for use with Revision D 01 06 Maintenance Apparatus 3 Apparatus 7 Operator s Manual P N 70 9039 Clean System Function with Peristaltic Pump Use the clean system function often to keep the valves needles and sample lines clean The timing of the valves opening and closing with the pump rotations creates cavitation in the valves Cleaning the system dislodges any particulate matter that may affect the operation of the system Not
62. mp contains a valve to control the direction of the flow of medium Use this option to move the valve to the input position to manually input or fill the syringe with medium Select option 1 Valve to Input to move the valve to the input position The valve emits a loud click when it moves If you hear no sound the valve is already in the input position 2 Valve to Output The syringe pump contains a valve to control the direction of the flow of medium Use this option to move the valve to the output position to manually output or purge the syringe of medium Select option 2 Valve to Output to move the valve to the output position The valve emits a loud click when it moves If you hear no sound the valve is already in the output position 3 Syringe Fill Use this option to draw a specific amount of medium into the syringes Ensure the valve is in the input position before drawing the medium see 1 Valve to Input above Select option 3 Syringe Fill Enter the desired percentage of each syringe to fill and press ENTER The syringes draw the specified amount of medium 4 Syringe Dispense 5 Filter Changer Use this option to dispense medium from the syringes Select option 4 Syringe Dispense The syringes dispense the previously drawn medium see 3 Syringe Fill above Use this option to manually operate the filter changer Select option 5 Filter Changer The Filter Change Manual Control screen dis
63. mpact If not select option 1 to enable the impact printer Note select option 1 Impact or the instrument will not operate properly Varian Inc Page 44 VK 8000 for use with Revision D 01 06 Final Setup Apparatus 3 Apparatus 7 Operator s Manual P N 70 9039 Key Sequence Function MENU gt 0 gt F Use this key sequence to select the date display style The asterisk displays next to the current selection Select option 1 MM DD YY or option 2 DD MM YY MENU gt 0 gt P Use this key sequence to enable or disable the syringe pump The asterisk displays next to the current selection If your system configuration includes the syringe pump you must enable the syringe pump control options on the VK 8000 prior to setting the syringe pump parameters See Main Menu Option 3 Set Syringe Pump Syringe Pump on page 54 Note the syringe pump option is not available for use with the 12 row 12 position Apparatus 7 MENU gt 0 gt R Use this key sequence to enable or disable media replacement for the syringe pump Ensure the asterisk displays next to option 2 Disable If not select option 2 to disable the media replacement for the syringe pump Note the media replacement option is not available with the Apparatus 3 Apparatus 7 Ensure the option is disabled or the instrument will not operate properly Varian Inc Chapter 8 Operating the VK 8000 VK 8000 Keypa
64. n the workplace for North America Indicates that this product must not be disposed of as unsorted municipal waste see WEEE Directive on page 14 Varian Inc Revision D 01 06 VK 8000 for use with Page 13 P N 70 9039 Apparatus 3 Apparatus 7 Operator s Manual Safety Practices and Hazards General CE Compliant Products The VK 8000 and accessories have been designed to comply with the requirements of the Electro magnetic Compatibility EMC Directive and the Low Voltage Directive LVD of the EU Varian Inc has confirmed that each product complies with the relevant directives by testing a prototype against the prescribed European Norm EN standards Proof that a product complies with the directives is indicated by e the CE marking appearing on the rear of the product e the documentation package that accompanies the product containing a copy of the declaration of conformity This declaration is the legal declaration by Varian Inc that the product complies with the directives and also shows the EN standards to which the product was tested to demonstrate compliance The declaration of conformity is signed by the representative of the manufacturing plant cTUVus U S and Canadian Product Approvals The VK 8000 and accessories have been designed to comply with North American safety requirements These products have been tested and certified for the North American market by TUV Rheinland of North America Inc Th
65. nd the baud rate Press PRINT Enter a program number and press ENTER The Print Selections screen displays PRINT SELECTIONS 1 BATCH INFORMATION 2 SAMPLE TIMES 3 TURN ON OFF REP CENTER 4 SET COM PORT ID 5 SET BAUD RATE Varian Inc Revision D 01 06 VK 8000 for use with Page 61 P N 70 9039 Apparatus 3 Apparatus 7 Operator s Manual Operating the VK 8000 Following is a description of the Print Selections screen options Option Function 1 Batch Information Select option 1 Batch Information to immediately print the previously entered batch information if any for the selected program This printed data is the header information you can enter via option 4 Headers on the Program Variables screen see 4 Headers on page 63 2 Sample Times Select option 2 Sample Times to immediately print the sampling times for each row of the selected program This is the complete listing of all timepoints entered in option 2 Sample Time Points under the Program Variables screen see Program Option 2 Sample Time Points on page 66 3 Turn On Off Rep Select option 3 Turn On Off Rep Center to enable or disable the Center Report Center Printer This option always reflects the opposite of the current state of the Report Center Printer For example if it is currently disabled TURN ON REP CENTER displays next to option 3 Note the state of the Report Center Printer is not unique to an individ
66. ng is a description of the Program Variables screen options Option Function 1 Set BIO DIS Use this option to set the Apparatus 3 Apparatus 7 parameters Parameters Select option 1 Set BIO DIS Parameters The Set BIO DIS Parameters screen displays See Main Menu Option 2 Set BIO DIS on page 53 for a description of the Set BIO DIS Parameters screen options 2 Sample Time Use this option to enter the sample timepoints See Program Option 2 Points Sample Time Points on page 66 3 Sample Volume 4 Headers Select option 3 Sample Volume Enter the sample volume in milliliters and press ENTER The Program Variables screen displays You can enter the volume with up to three digits for example 13 5 so that fractional milliliters can be collected Verify the collection tubes can hold the requested volume Note the maximum volume accepted is 14 mL Select option 4 Headers The Batch Selections screen displays For each option enter the desired information and press ENTER The Program Variables screen displays Note the Batch Selections screen displays options for the report header information The print field accepts ten characters Characters can be letters numbers special characters or spaces The information entered for each selection prints in the header of each printout The entries made from this menu are attached to the active program Since there are 15 programs there can be a different se
67. o programs with identical test parameters back to back To link identical programs link the program to itself Varian Inc Page 68 VK 8000 for use with Revision D 01 06 Operating the VK 8000 Apparatus 3 Apparatus 7 Operator s Manual P N 70 9039 To link another program to the one previously selected select option 2 The following screen displays LINK PROG 1 15 TIME 08 29 04 15 02 30 ELAPSE 000 00 04 Enter the program number to link to the current program and press ENTER Note When running two programs it is necessary to replace the full G E sample tray with an empty one between programs Enable the program completion alarm see 5 Prg Compl Alarm on page 52 to alert the analyst when the first program completes and the second program begins Step 4 The Select Start Mode screen displays Options 1 3 start the collection program Option 4 allows you to change or review the program before beginning SELECT START MODE 1 START NOW 2 DELAYED START 3 REMOTE START 4 MODIFY PROGRAM PROG 1 R M DISABLED VOL 5ml Following is a description of the Select Start Mode screen options Option Function 1 Start Now Select option 1 Start Now The BIO DIS Options screen displays See BIO DIS Options Screen on page 69 2 Delayed Start Select option 2 Delayed Start The BIO DIS Options screen displays See BIO DIS Opti
68. on includes the peristaltic pump use this option to set the interval the peristaltic pump runs in reverse to return the uncollected medium in each line back into the vessel from which it was drawn Select option 6 Purge Time Enter a value for the purge time from 1 to 99 seconds and press ENTER The Program Variables screen displays Note purging also clears the sampling filters of particulate matter that may restrict successive samples As with priming the longer the tubing lengths the longer the purge time required Varian Inc Revision D 01 06 VK 8000 for use with Page 65 P N 70 9039 Apparatus 3 Apparatus 7 Operator s Manual Operating the VK 8000 Option Function 6 Purge Volume syringe pump If your system configuration includes the syringe pump use this option to set a purge volume that ensures all stranded medium is properly expelled Select option 6 Purge Volume Enter a value for the purge volume that ensures at least two strokes of the syringe plunger and press ENTER The Program Variables screen displays Note the first stroke moves all medium from the sampling lines to the return lines The second stroke draws air into the sampling lines and purges the remaining medium from the return lines As with priming the longer the tubing lengths the larger the purge volume required 7 R M peristaltic pump If your system configuration includes the peristaltic pump select option 7 R M Th
69. ons Screen on page 69 Varian Inc Revision D 01 06 P N 70 9039 VK 8000 for use with Page 69 Apparatus 3 Apparatus 7 Operator s Manual Operating the VK 8000 Option Function 3 Remote Start This option is designed for use with the VK 8000 coupled with non Varian instruments Contact the Dissolution Systems Service Department for information about this function 4 Modify Program Select option 4 Modify Program to modify or review the selected program See Programming the VK 8000 on page 62 to review programming options All program variable items can be viewed or changed Press ESC to return to the Select Start Mode screen Selecting option 4 again repeats the modify program operation When the program is verified or changed to the correct settings select the desired start mode option 1 3 to start the program BIO DIS Options Screen BIO DIS OPTIONS 1 LOAD amp RUN BIO DIS 2 RUN WO LOADING 3 RUN VK 8000 ONLY 4 SET BIO DIS TIME 08 29 04 08 09 51 ELAPSE 000 00 00 Following is a description of the BIO DIS Options screen options Option Start Now Function Delayed Start Function 1 Load amp Select option 1 Load amp Select option 1 Load amp Run BIO DIS to upload all Run Run BIO DIS to upload all saved parameters to the Apparatus 3 Apparatus 7 BIO DIS saved parameters to the Enter the start time in 24 hour format and press
70. op of the unit The sample tray has four grommeted corner holes which fit over the four locating pins Caution The sample tray must be securely mounted on the locating pins Otherwise damage can occur to the sampling needles and drive mechanism Installing the Media Rinse Reservoir The media rinse reservoir fits in the rear of the unit between the metal brackets The dispensing arm must be moved forward to put it in place Step 1 From the Ready screen press MENU Step 2 Select option 7 Manual Operation Step 3 Select option 4 Goto Rows Step 4 Enter 4 to move the dispensing arm to row 4 The dispensing arm moves forward to allow access to the rear of the VK 8000 Step 5 Place the media rinse reservoir between the metal brackets directly behind the sample tray Note The media rinse reservoir has two chambers one for gt replacement media and one for rinsing The replacement media chamber has two barbed fittings one on each end of the tank The rinse chamber has a single barbed fitting Orient the reservoir with the rinse chamber toward the front Varian Inc Page 42 Final Setup VK 8000 for use with Revision D 01 06 Apparatus 3 Apparatus 7 Operator s Manual P N 70 9039 Step 6 Step 7 Connect the six foot length of clear tygon tubing to the barbed elbow fitting located on the rinse chamber of the reservoir Place the other end in a sink drain or suitabl
71. paratus 7 Operator s Manual Unpacking and Positioning Setting Up Your Sampling System This manual provides setup instructions for all configurations using the VK 8000 with an Apparatus 3 Apparatus 7 All setup combinations are described in individual chapters Refer to the following list and read the appropriate chapter for your system configuration e Setting Up Your VK 8000 with Peristaltic Pump on page 25 e Setting Up Your VK 8000 with Syringe Pump on page 29 e Setting Up Your VK 8000 with Syringe Pump and Filter Changer on page 33 Warning For all configurations ensure the equipment is configured for the voltage supplied Varian Inc Chapter 4 Setting Up Your VK 8000 with Peristaltic Pump Setting Up the Peristaltic Pump Step 1 Remove the two screws on top of the pump Lift off the plastic cover and set it aside Step 2 Remove the cartridges from the pump by pressing down the levers on the right side Lift the cartridges up and away from the pump Step 3 Loosely fit the peristaltic pump tubing along the inside groove of the first cartridge locking the tubing clips on the outside notches Note Ensure that all pump cartridges are installed It is not necessary to install tubing on any unused positions qe Step 4 Refit the cartridge to the pump by hooking the clip on the bottom left of the cartridge into the groove on the left side of the pump Varian Inc
72. plays To open the filter clamp select option 1 Open Clamp To close the filter clamp select option 2 Close Clamp To change the filters select option 3 Change Filters Enter the number of filters to change and press ENTER The specified number of filters are expelled and replaced Press ESC to return to the Operate Syringe Filter Changer screen Varian Inc Page 60 VK 8000 for use with Revision D 01 06 Operating the VK 8000 Apparatus 3 Apparatus 7 Operator s Manual P N 70 9039 Option Function 6 Syringe Parameters Use this option to configure the syringe pump Select option 6 Syringe Parameters The Set Syringe Pump Parameters screen displays For a description of the screen options see Main Menu Option 3 Set Syringe Pump Syringe Pump on page 54 Note the syringe pump parameters are global These parameters can be entered under option 3 of the Main Menu or option 6 of the Operate Syringe Filter Changer screen Printer Operation and Communications Note I a Ensure the printer type is set to impact for the instrument to operate properly see MENU gt 0 gt E under Hidden Key Functions on page 43 Use print options to e print batch information and sample times for each of the 15 stored programs e control the built in Report Center Printer and the remote printer if one is connected e set the communication port identification number a
73. point is reached in each well the valve closes and the time value is recorded in memory When the last valve closes the pump reverses and purges the lines The calibration completion alarm if enabled sounds when the calibration sequence is finished Press any key to silence the alarm Note Because of intrinsic variations in tubing diameters internal valve passages and pump tubing tensions some valves must stay open longer than others to deliver their nominal 10 mL calibration volumes This is normal operation Step 7 Step 8 Step 9 Step 10 After the dispensing arm moves forward remove the autocalibration block Clean and dry the separate portions of the autocalibration block Reconnect the top and bottom portions of the autocalibration block and replace it Press ESC to return the dispensing arm to the home position Varian Inc Revision D 01 06 VK 8000 for use with Page 75 P N 70 9039 Apparatus 3 Apparatus 7 Operator s Manual Operating the VK 8000 Manual Calibration To perform the calibration yourself complete the following steps Step 1 Remove the sample tray Step 2 Fill a row of outer media tubes with tap water Step 3 Ensure the correct prime and purge times have been entered via the Main Menu option 4 and 5 Manual Prime Time and Manual Purge Time respectively see 4 Manual Prime Time peristaltic pump on page 49 and 5 Manual Purge Time peristaltic pump
74. pparatus 3 Apparatus 7 drive unit moves to the indicated row 4 Home Select option 4 Home to move the drive unit to the home position Press ESC to return to the Main Menu Main Menu Option 7 Manual Operation From the Main Menu select option 7 Manual Operation The following Manual Operations screen displays when your system configuration includes the peristaltic pump MANUAL OPERATIONS 1 LIFT VALVES 2 LOWER VALVES 3 OPEN CLOSE VALVES 4 GOTO ROWS 5 TURN ON OFF R M PUMP The following Manual Operations screen displays when your system configuration includes the syringe pump with or without the filter changer MANUAL OPERATIONS 1 LIFT VALVES 2 LOWER VALVES 3 OPEN CLOSE VALVES 4 GOTO ROWS 5 TURN ON OFF R M PUMP 6 SYRINGE FILTER All functions execute immediately without pressing ENTER Press ESC to return to the Main Menu Varian Inc Page 58 Operating the VK 8000 VK 8000 for use with Revision D 01 06 Apparatus 3 Apparatus 7 Operator s Manual P N 70 9039 Following is a description of the Manual Operations screen options Option Function 1 Lift Valves Select option 1 Lift Valves to raise the valve needles if lowered 2 Lower Valves Select option 2 Lower Valves to lower the valve needles if raised 3 Open Close Valves Select option 3 Open Close Valves to toggle the valves open and closed 4 Goto Rows Sel
75. r use with Page 49 P N 70 9039 Apparatus 3 Apparatus 7 Operator s Manual Operating the VK 8000 Option Function 4 Manual Prime Time peristaltic pump If your system configuration includes the peristaltic pump use this option to set the interval the peristaltic pump runs forward drawing medium into the system before the valves open to deliver samples Select option 4 Manual Prime Time Enter a value for the priming time from 1 to 99 seconds and press ENTER The Main Menu displays Note at a minimum the manual prime time should be long enough for medium to be seen dripping from the return cannulas In general the longer the tubing lengths the longer the priming time required This value is not used by sampling programs which have their own individual priming time values It is used only with the MANUAL SAMPLE CAL and CLEAN SYSTEM keys on the VK 8000 4 Manual Prime Vol syringe pump If your system configuration includes the syringe pump use this option to set the amount of drawn medium necessary to fill the sampling lines of the entire system Select option 4 Manual Prime Vol Enter a value for the priming volume and press ENTER The Main Menu displays Note at a minimum the manual prime volume should be large enough for medium to be seen dripping from the return cannulas In general the longer the tubing lengths the larger the priming volume required This value is not used by sampling programs whic
76. r use with Revision D 01 06 Service and Warranty Apparatus 3 Apparatus 7 Operator s Manual P N 70 9039 Exclusions and Limitations Excluded from this warranty are expendable or consumable items such as but not limited to paddles baskets vessels and acrylic water baths Also excluded are defects from improper or inadequate maintenance by the customer user induced chemical action or contamination unauthorized modification or misuse and improper site preparation and maintenance Operation of software is not warranted to be uninterrupted or error free Obtaining Warranty Service To obtain warranty service in the United States contact the Dissolution Systems Service Department at 800 229 1108 to obtain authorization to return units for repair At the option of the customer onsite warranty service is available but travel charges may be incurred The customer should prepay all shipping charges for products returned to the Dissolution Systems Service Department unless otherwise authorized and Varian Inc will pay all charges for return to the customer Warranty Limitations Varian Inc makes no other warranty either express or implied with respect to this product Specifically disclaimed are any implied warranties of merchantability and fitness for a particular use In no event will Varian Inc be liable for any indirect incidental or consequential damages arising from the use of this product This warranty gives you speci
77. ry or painful electric shock Panels or covers that are retained by fasteners which require the use of a tool for removal may be opened only by Varian trained Varian qualified or Varian authorized service engineers Consult the manuals or product labels supplied with the VK 8000 and accessories to determine which parts are operator accessible Application of the wrong supply voltage connection of the instrument to an incorrectly wired supply outlet or lack of proper electrical grounding can create a fire hazard or a potentially serious shock hazard and could seriously damage the instrument and any attached ancillary equipment Always use a three wire outlet with ground connection which is adequately rated for the load The installation must comply with local state and federal safety regulations Do not connect the instrument to the main power supply until you have made sure that the operating voltage is correctly set for the main power supply in the specific outlet in your laboratory to which the equipment will be connected Other Other specific warnings and cautions appear in the manuals where appropriate and detail the specific hazard describe how to avoid it and specify the possible consequences of not heeding the warning or caution Warning A Warning message appears in the manual when failure to observe instructions or precautions could result in death or injury Symbols depicting the nature of the specific hazard are a
78. ry pull back the plunger and carefully remove it from the syringe barrel y metal fitting syringe barrel seal plunger plunger cap Step 2 Remove the old seal from the plunger by carefully cutting into the seal until the seal peels easily away Discard the old seal Varian Inc Revision D 01 06 VK 8000 for use with Page 89 P N 70 9039 Apparatus 3 Apparatus 7 Operator s Manual Maintenance Step 3 Remove and discard the O ring Step 4 Place the new O ring from the refurbishing kit onto the plunger Step 5 Place the new seal on a hard flat surface Step 6 Push the plunger into the seal Step 7 Ensure the seal is tight at all points around the plunger Step 8 Replace the plunger see Replacing the Syringe on page 90 Step 9 Clean the syringe see Cleaning the Syringes on page 91 Varian Inc Page 90 VK 8000 for use with Revision D 01 06 Maintenance Apparatus 3 Apparatus 7 Operator s Manual P N 70 9039 Replacing the Syringe Complete the following steps to replace the syringe Step 1 Wet the plunger seal and insert the seal into the syringe barrel see photo on page 88 Ensure the plunger cap is firmly in place inside the syringe barrel Note qd If the plunger seal is not wetted before it is inserted into the syringe it does not seal properly and will leak Step 2 Pull back the plunger after it is inserted Zz threaded mo
79. s all medium from the sampling lines to the return lines The second stroke draws air into the sampling lines and purges the remaining medium from the return lines As with priming the longer the tubing lengths the larger the purge volume required This value is not used by sampling programs which have their own individual purge volume values It is used only with the MANUAL SAMPLE and CLEAN SYSTEM keys on the VK 8000 6 Control BIO DIS Use this option to remotely control the Apparatus 3 Apparatus 7 from the VK 8000 See Main Menu Option 6 Control BIO DIS on page 56 7 Manual Operation Use this option to manually control the VK 8000 See Main Menu Option 7 Manual Operation on page 57 Varian Inc Revision D 01 06 VK 8000 for use with Page 51 P N 70 9039 Apparatus 3 Apparatus 7 Operator s Manual Operating the VK 8000 Main Menu Option 1 Set Clock Alarms From the Main Menu select option 1 Set Clock Alarms The Set Clock and Alarms screen displays SET CLOCK AND ALARMS 1 SET CLOCK 2 TIMER ALARM 3 ROW COMPL ALARM 4 CALIB COMPL ALARM 5 PRG COMPL ALARM 6 START OUTPUT DELAY Following is a description of the Set Clock and Alarms screen options Option Function 1 Set Clock Select option 1 Set Clock Enter the date in the appropriate format and press ENTER The time displays in 24 hour format If the time is correct press ENTER or ESC to return to the Main Menu If the t
80. t of entries for each All are stored in the battery protected memory and remain until changed via this menu Varian Inc Page 64 VK 8000 for use with Revision D 01 06 Operating the VK 8000 Apparatus 3 Apparatus 7 Operator s Manual P N 70 9039 Option Function 5 Prime Time peristaltic pump 5 Prime Volume syringe pump If your system configuration includes the peristaltic pump use this option to set the interval the peristaltic pump runs forward drawing medium into the system before the valves open to deliver samples Select option 5 Prime Time Enter a value for the priming time from 1 to 99 seconds and press ENTER The Program Variables screen displays Note at a minimum the prime time should be long enough for medium to be seen dripping from the return cannulas In general the longer the tubing lengths the longer the priming time required If your system configuration includes the syringe pump use this option to set the amount of drawn medium necessary to fill the sampling lines of the entire system Select option 5 Prime Volume Enter a value for the priming volume and press ENTER The Program Variables screen displays Note at a minimum the prime volume should be large enough for medium to be seen dripping from the return cannulas In general the longer the tubing lengths the larger the prime volume required 6 Purge Time peristaltic pump If your system configurati
81. tep 1 Connect the single end of the Y cable 1 P N 5075 0446 to the RS232 port on the syringe pump Step 2 Connect an RS232 cable 2 P N 5075 0252 between the RS232 port on the VK 8000 and one extension of the Y cable attached to the syringe pump Step 3 Connect a second RS232 cable 2 P N 5075 0252 between the remaining extension of the Y cable attached to the syringe pump and the RS232 port on the Apparatus 3 Apparatus 7 Varian Inc Revision D 01 06 VK 8000 for use with Page 31 P N 70 9039 Apparatus 3 Apparatus 7 Operator s Manual Setting Up Sample Tubing Connections Note q a Do not remove the nylon tie wraps securing the tubing into bundles Step 1 Connect the 72 inch multi color Teflon lines from the Apparatus 3 Apparatus 7 to the left port of each syringe starting with position 1 Step 2 Connect the 32 inch multi color Teflon lines between the right port of each syringe to the tubing coming from the left positions of the VK 8000 solenoid valves Ensure each line is attached to its identical color Step 3 Connect the tubing coming from the top positions of the VK 8000 solenoid valves to the 72 inch multi color Teflon return lines on the Apparatus 3 Apparatus 7 Ensure each line is attached to its identical color Caution Do not tighten the plastic fittings on the ends of the tubing more than half a turn past finger tight Overtightening can damage the fittings
82. tep 4 Enter the following parameters Selection Response 1 Dipping Speed DPM Enter 10 and press ENTER for all rows 2 Samples Per Row Enter 3 and press ENTER Enter 0 and press ENTER for all remaining rows 3 Hold Dip Time Enter 00 00 and press ENTER for all rows Varian Inc Page 94 VK 8000 for use with Revision D 01 06 Maintenance Apparatus 3 Apparatus 7 Operator s Manual P N 70 9039 Selection Response 4 Set Bath Temp Enter 37 3 and press ENTER 5 Com Port ID Enter 01 and press ENTER 6 Print Frequency Enter 000 and press ENTER Step 5 Press ESC gt Y The Program Variables screen displays Step 6 Enter the following parameters Selection Response 2 Sample Time Points Row 0 Enter 000 05 and press ENTER Row 1 Enter 000 10 and press ENTER Row 2 Enter 000 15 and press ENTER Row 3 Enter 000 00 and press ENTER 3 Sample Volume Enter a sample volume based on the size vial used in the VK 8000 sample tray and press ENTER Ensure rows 0 2 in the sample tray contain empty vials 4 Set Headers Enter the requested information as applicable Press ESC to return to the Program Variables screen 5 Prime Volume Enter 10 and press ENTER 6 Purge Volume Enter 15 and press ENTER 7 Filter Chg This option is only applicable if your system configuration includes a filter changer Set the number of samples per filter to 1 and press ENTER Step 7 Pr
83. testers and VK 8000s can be started at the same time using one VK 8000 as the master unit To set up a daisy chain complete the following steps Step 1 Connect the master VK 8000 START OUTPUT jack to the START INPUT jack of its dedicated Apparatus 3 Apparatus 7 using a four pin cable Connect the START OUTPUT jack of the first Apparatus 3 Apparatus 7 to the START INPUT jack of a second VK 8000 Connect the START OUTPUT jack of the second VK 8000 to the START INPUT jack of the second Apparatus 3 Apparatus 7 Daisy chain successive units using the same technique Step 2 Set the desired program for each VK 8000 independently Varian Inc Revision D 01 06 VK 8000 for use with Page 79 P N 70 9039 Apparatus 3 Apparatus 7 Operator s Manual Operating the VK 8000 Step 3 For each of the remote units Note qE Ensure the communication port identification number for each linked VK 8000 increases incrementally see 4 Set Com Port ID under Printer Operation and Communications on page 61 Press START PROG Enter the desired program number and press ENTER Select option 1 Run Single Prog Select option 3 Remote Start Select option 1 Load amp Run BIO DIS The program loads and the Ready screen displays Step 4 For the master VK 8000 Press START PROG Enter the desired program number and press ENTER Select option 1 Run Single Prog Select option 1 Start Now Select option 1 Load a
84. the outer media tubes with ultrapure water Repeat steps 6 10 to flush the syringe Change the cleaning solution in the outer media tubes to 0 1N HCI and repeat steps 6 10 Replace the cleaning solution in the outer media tubes with ultrapure water Varian Inc Revision D 01 06 VK 8000 for use with Page 93 P N 70 9039 Apparatus 3 Apparatus 7 Operator s Manual Maintenance Step 14 Repeat steps 6 10 a minimum of ten times to flush the syringes thoroughly Step 15 When the cleaning procedure is complete press ESC until the Ready screen displays System Cleaning Program with Syringe Pump and Filter Changer optional To clean the VK 8000 syringe pump and filter changer system use the appropriate cleaning solution based on the product being tested Remove the inner tube assemblies from the adapter shafts Use a row of 300 mL outer media tubes on the Apparatus 3 Apparatus 7 to hold the cleaning solution and ensure the sampling cannulas are submerged into the cleaning solution Complete the following steps to set up the cleaning procedure Note I gt Ensure the RM option is disabled using the hidden key function MENU gt 0 gt R Step 1 From the Ready screen press PROG to program the VK 8000 Step 2 Enter a program number 1 15 to use for this cleaning program and press ENTER The Program Variables screen displays Step 3 Select option 1 Set BIO DIS Parameters S
85. the single end of a second Y cable 1 P N 5075 0446 to the RS232 port on the syringe pump Varian Inc Revision D 01 06 VK 8000 for use with Page 35 P N 70 9039 Apparatus 3 Apparatus 7 Operator s Manual Setting Up Step 5 Connect one extension of the second Y cable to the remaining end of the nine pin cable attached to the filter changer 3 P N 5075 0449 from step 3 Step 6 Connect a second RS232 cable 2 P N 5075 0252 between the RS232 port on the Apparatus 3 Apparatus 7 and the remaining extension of the Y cable attached to the syringe pump from step 4 Sample Tubing Connections Note Do not remove the nylon tie wraps securing the tubing into bundles Step 1 Step 2 Step 3 Connect the 72 inch multi color Teflon lines from the Apparatus 3 Apparatus 7 to the left port of each syringe starting with position 1 Connect the 28 inch multi color Teflon lines between the right port of each syringe and the top inlet ports of the filter changer starting with the port closest to the carousel Connect the 32 inch multi color Teflon lines between the side ports of the filter changer and the tubing coming from the left positions of the VK 8000 solenoid valves Ensure each line is attached to its identical color Varian Inc Page 36 VK 8000 for use with Revision D 01 06 Setting Up Apparatus 3 Apparatus 7 Operator s Manual P N 70 9039 Step 4 Connect
86. the tubing coming from the top positions of the VK 8000 solenoid valves to the 72 inch multi color Teflon return lines on the Apparatus 3 Apparatus 7 Ensure each line is attached to its identical color Caution Do not tighten the plastic fittings on the ends of the tubing more than half a turn past finger tight Overtightening can damage the fittings Loading Filters into the Filter Changer Step 1 Choose a tube of filters Although you can load up to eight tubes of filters only one tube is necessary for the filter changer to function properly Step 2 Remove the cap from the end of the tube to expose the long thin exit nozzle of the end filter Step 3 Insert the tube with the open end down into the metal filter assembly in the filter changer carousel Pivot the tube until it slides vertically into the white stabilizer slot Step 4 Repeat steps 2 4 to load additional tubes of filters Caution Use only Varian certified filters or Millipore equivalent types of filters Other types of filters may not work properly in the filter changer and could damage the unit Varian Inc Revision D 01 06 VK 8000 for use with Page 37 P N 70 9039 Apparatus 3 Apparatus 7 Operator s Manual Setting Up Changing Filter Types To change filter types in the VK 808 Filter Changer complete the following steps Step 1 Remove all tubes of filters from the carousel of the filter changer Step 2 From the VK 8000 R
87. time in 24 hour format and press VK 8000 only The ENTER Enter the start date and press ENTER Once VK 8000 executes the the start date and time are reached the VK 8000 program currently selected executes the program currently selected This option is used when If the delayed start alarm is enabled enter the other instrumentation such duration before the start of the program you would like as an Apparatus 3 the alarm to sound To disable the alarm see MENU gt Apparatus 7 is connected 0 gt C under Hidden Key Functions on page 43 to the VK 8000 through the A l t any time prior to the scheduled start of the START OUTPUT jack on 3 program press ESC to cancel the delayed start the rear of the instrument 4 Set Use this option to modify or review the current program parameters Select option 4 BIO DIS Set BIO DIS The Set BIO DIS Parameters screen displays See Main Menu Option 2 Set BIO DIS on page 53 for a description of the Set BIO DIS Parameters screen options Varian Inc Revision D 01 06 VK 8000 for use with Page 71 P N 70 9039 Apparatus 3 Apparatus 7 Operator s Manual Operating the VK 8000 Manual Sample From the Ready screen press MANUAL SAMPLE to collect a sample from the outer media tubes on command without a program These options are only for manual sampling and have nothing to do with the stored program parameters for purge volume prime volume and so on When your system confi
88. tput delay option also displays on this screen See Main Menu Option 1 Set Clock Alarms on page 51 Use this option to set the Apparatus 3 Apparatus 7 parameters See Main Menu Option 2 Set BIO DIS on page 53 3 Start Output Delay peristaltic pump 3 Set Syringe Pump syringe pump If your system configuration includes the peristaltic pump use this option to set the delay from the start of a program until the TTL signal is sent from the START OUTPUT jack on the VK 8000 rear panel Select option 3 Start Output Delay Enter a delay length of up to 99 59 minutes for the VK 8000 TTL output and press ENTER The Main Menu displays Note this output signal can be used to start other instrumentation such as an Apparatus 3 Apparatus 7 after the program on the VK 8000 is started The default delay is 00 00 indicating that the signal will be sent at the moment a program starts For example a value of 10 00 delays the signal for ten minutes after the program starts Set the value to 00 00 to eliminate the delay feature When the syringe pump is enabled Start Output Delay displays under the Set Clock and Alarms screen as option 6 See 6 Start Output Delay on page 52 If your system configuration includes the syringe pump use this option to set the syringe pump parameters See Main Menu Option 3 Set Syringe Pump Syringe Pump on page 54 Varian Inc Revision D 01 06 VK 8000 fo
89. type expels from the filter changer and is replaced with the new filter Step 15 Press ESC four times to return to the Ready screen Installing the Catch Basin Slide the side of the catch basin containing the opening over the screw mounted on the front of the filter changer Varian Inc Chapter 7 Final Setup Power and Network Connections Warning The electrical connection at the back of the VK 8000 is the primary disconnect for the instrument The instrument should be positioned to allow accessibility to the power cords for easy disconnection Ensure the VK 8000 and any coupled components are configured for the voltage supplied at the receptacle e f your system configuration includes the peristaltic pump the power receptacle and power switch are located on the rear of the unit Connect the power cord and turn on the peristaltic pump e f your system configuration includes the syringe pump the power receptacle and power switch are located on the left side of the unit Connect the power cord and turn on the syringe pump e f your system configuration includes the filter changer the power receptacle and power switch are located on the rear of the unit Connect the power cord and turn on the filter changer Varian Inc Page 40 VK 8000 for use with Revision D 01 06 Final Setup Apparatus 3 Apparatus 7 Operator s Manual P N 70 9039 The VK 8000 has a dual voltage 115 V 230 V option on the
90. ual program It is global When you enable or disable it the Report Center Printer stays enabled or disabled until you change it again even if you switch programs 4 Set Com Port ID Select option 4 Set Com Port ID to enter a new communication port identification number Your system was shipped with the communication port identification number set to 01 You can set the identification number to any number between 1 and 99 The identification number is used when multiple units are connected in series so that the controlling or master VK 8000 can start individual units Enter the communication port identification number and press ENTER The Print Selections screen displays 5 Set Baud Rate Select option 5 Set Baud Rate to set the baud rate for your system If you are using the VK 8000 with other Varian equipment set the baud rate to 9600 baud option 4 Select the desired baud rate from the menu The Print Selections screen displays Note in order to set the baud rate from this option the baud rate must be set to programmable baud see MENU gt 0 gt 8 under Hidden Key Functions on page 43 Varian Inc Page 62 VK 8000 for use with Revision D 01 06 Operating the VK 8000 Apparatus 3 Apparatus 7 Operator s Manual P N 70 9039 Programming the VK 8000 Note Programs are not write protected Check with other users before proceeding _ Step 1 From the
91. unting pin plunger cap plunger notch Step 3 Align the plunger notch with the pin drive Step 4 Turn the metal fitting on top of the syringe barrel clockwise so the threaded mounting pin screws into place under the solenoid valve Step 5 Screw in the locking screw at the base of the plunger Step 6 Use pliers to firmly tighten the metal fitting at the top of the syringe barrel Step 7 Press 4 to dispense the syringes and move the syringe plungers to their highest position Step 8 Press ESC until the Ready screen displays Varian Inc Revision D 01 06 VK 8000 for use with Page 91 P N 70 9039 Apparatus 3 Apparatus 7 Operator s Manual Maintenance Cleaning the Syringes To clean the syringes use the appropriate cleaning solution based on the product being tested Move the Apparatus 3 Apparatus 7 drive unit to the row of outer media tubes which contain the cleaning solution and begin dipping Syringes should be cleaned after each test using one of the following procedures 50 Methanol or 10 Bleach Cleaning Procedure Step 1 From the VK 8000 Ready screen press MENU gt 7 The Manual Operations screen displays Step 2 Select option 6 Syringe Filter The Operate Syringe Filter Changer screen displays Step 3 Select option 1 Valve to Input to ensure the valve is set to input Step 4 Select option 3 Syringe Fill Step 5 Enter 100 and press ENTER The syringes fill completely with cleaning
92. ween the jack on the VK 8000 rear panel labeled PUMP and the jack on the peristaltic pump rear panel labeled PUMP CONTROL Step 2 Connect the RS232 cable 1 P N 5075 0042 between the RS232 ports on the Apparatus 3 Apparatus 7 side panel and the VK 8000 rear panel Varian Inc Page 28 Setting Up VK 8000 for use with Revision D 01 06 Apparatus 3 Apparatus 7 Operator s Manual P N 70 9039 Sample Tubing Connections The VK 8000 is shipped with all tubing attached to the valves in the dispensing arm Note Do not remove the nylon tie wraps securing the tubing into bundles QE gt The direction of the peristaltic pump can be set via a switch on its rear panel It does not matter at this point to which side the bundles go as long as all lines in the bundle from the sampling cannulas are connected to one side and all lines in the bundle from the valve inlets to the other Step 1 Step 2 Step 3 Connect the 72 inch multi color Teflon lines from the Apparatus 3 Apparatus 7 to the tubing on one side of the appropriate peristaltic pump cartridges For the 6 row apparatus connect the 32 inch multi color Teflon lines between the remaining side of the peristaltic pump cartridges and the tubing coming from the left positions of the VK 8000 solenoid valves Ensure each cartridge has identical color tubing connected at both the inlet and the outlet For the 12 row apparatus connect the 32

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