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41091001 M410 & 410C with Interface Operator Manual Issue 7

1. Disconnect the mixing chamber from the chimney assembly by twisting it to the right through 40 degrees Lower the mixing chamber and burner assembly Lift the burner tube from the mixing chamber and remove the burner Unscrew the end cap securing screw item 6 Figure 6 1 Grasp the end cap item 3 Figure 6 1 and remove it from the mixing chamber with a slightly twisting action Lift the end cap to allow the water in the U tube to flow into the constant head drain Disconnect the U tube from the end cap and constant head drain Unclip the constant head drain and disconnect the drain tube Soak all the items removed from the instrument in a 1 in 100 dilution of Cleaning Solution agitating it periodically Rinse the parts thoroughly in deionised water and dry with clean tissues Fit the burner tube to the mixing chamber ensuring that the locating slot in the burner tube engages with the locating pin in the mixing chamber Fit the burner pointed end downwards into the burner tube Rotate the burner to ensure that it is fully inserted Check the condition of the end cap 0 ring seal If necessary replace the seal Replace the end cap using a twisting movement Align the hole with the mixing chamber screw fixing Fit the screw item 6 Figure 6 1 Carefully position the mixing chamber with the burner tube inside the chimney With the burner tube fully inserted twist the mixing chamber to the left through 40 de
2. 410 91 001 Issue 7 24 March 2012 ECN 736 50 Model 410 amp 410C Flame Photometer with Interface Appendix B continued 3 6 Sample requirement Serum plasma urine C S F and sweat Method of presentation The sample is either diluted using the following factors into a sample cup and is presented directly to the nebuliser inlet tube or it is diluted using the Model 805 Dilutor which is connected to the Model 410 via a weir cup assembly supplied with the Dilutor Sample Dilution Factor Min Volume Min volume manual dilution using M805 Na serum urine 1 200 10ul SOul K serum 1 200 10ul SOul K urine 1 100 10ul SOul see note Li serum 1 10 SOul N A Note For Potassium in urine the samples must be pre diluted 1 5 before using the M805 Note For Lithium determinations the blank solution should contain normal levels of Na K 4 4 4 5 and Ca to suppress the effect of mterference from these elements in the Lithium samples Operating Instructions When C on the Digital Interface is illuminated it indicates that the Lineariser 1s switched on and will operate when Na is selected To switch the Lineariser off make sure the Filter Selector is in the Na position then press and hold the Select button while moving the Filter Selector to the K position Release the Select button the buzzer will stop sounding and the C will no longer be illuminated To switch the Lineariser back on reverse the proc
3. Model 410 amp 410C Flame Photometer with Interface Installation continued 2 4 Assembly EQUIPMENT REQUIRED Pozidriv cross head screwdriver IPT Flat blade screwdriver To assemble the Model 410 proceed as follows l 410 91 001 Lift off the chimney cap from the chimney assembly and lift out the glass chimney Remove any packing material from inside making sure that there are no small pieces left within the chimney Replace the glass chimney with the clear strip at the bottom and refit the chimney cap on top of the chimney Remove the nebuliser from its box and push the nebuliser barb into the air tubing item 3 Figure 2 1 Fit the nebuliser to the mixing chamber and position the retainer to lock it into position item 2 Figure 2 1 Fit the nebuliser inlet tube making sure that it is pointing downwards Figure 2 1 Mixing Chamber 1 Nebuliser 2 Nebuliser retainer 3 Air tubing 4 Constant head drain 5 Fuel restrictor 6 Fuel tubing If the instrument is to be used on Natural gas refer to Appendix D If Propane LPG or Butane is to be used continue with paragraph 5 Issue 7 24 March 2012 ECN 736 Model 410 amp 410C Flame Photometer with Interface Installation continued 2 4 Assembly continued 5 410 91 001 Connect the length of rubberised fuel tubing 001 72 114 supplied between the inlet connector on the Propane regulator item 5 Figure 4 2 and the cylinder regulator ou
4. amp 410C Flame Photometer with Interface Appendix B Model 410 Clinical Addendum Using the M410 Flame Photometer on Clinical Samples These sections are replacement sections to those in the Main M410 Instruction Manual and should be read in conjunction with it 2 3 Introduction The Sherwood Model 410 Clinical Flame Photometer can be used for clinical samples serum plasma and urine Sherwood can provide two accessories which makes these measurements easier to carry out Clinical samples have high Sodium and Potassium concentrations which require significant dilution 1 in 200 Sherwood can supply a Continuous Flow Dilutor the Model 805 which performs this dilution automatically and can be directly connected to the Flame Photometer Even then the level of Sodium concentration is beyond the normal linear range of any flame photometer This usually means that a range of standards has to be made up and a curve plotted which is then used to determine the actual Sodium level of a sample Sherwood can provide a Lineariser which electronically adjusts the display according to well understood principles so that all levels of Sodium concentrations fall within the linear range of the instrument and only one standard and the zero value is required Unpacking substitute 00156100 140 Na 5 0 K 1 5 Li Standard x 100ml for 00156620 1000ppm Standard Na x 100ml x6 00156621 1000ppm Standard K x 100ml x6 00156623 1000ppm Standar
5. amp 410C Flame Photometer with Interface Operational Precautions and Limitations 5 1 General l Always dilute samples and standards with the same batch of diluent made up of 1 part Diluent Concentrate and 999 parts of deionised or good quality distilled water which contains non ionic wetting agents The samples should not be highly viscous or non homogeneous If possible samples likely to contain sediment should be filtered and then mixed to obtain a representative result Always use soap solution when checking for leaks in fuel or air lines Do not allow fuel to flow in the presence of unguarded flames e g cigarettes Always use genuine Sherwood Scientific replacement parts Do not for example replace the U tube with one of different material bore or length as this will cause a deterioration in the instrument s performance Always carry out the maintenance schedules as detailed in Section 6 Do not leave the inspection flap open unless adjusting flame conditions as this will allow stray light to enter the chimney The front panel of the Model 410 is impervious to a wide range of chemicals However strong acids and some organic solvents e g chloroform and phenol may affect the finish Any spillage should be thoroughly wiped away as soon as possible If necessary clean the instrument with warm soapy water do not use abrasives 5 2 Hazards l 410 91 001 All electrical instruments are potentially
6. calibration reading is within 1 If a chart recorder is being used set zero on the blank solution and set span while aspirating the calibration standard Without touching the fine and coarse controls aspirate each of the remaining calibration standards for 20 seconds starting with the lowest concentration to avoid carry over again allowing 10 seconds between measurements Note the value of each standard and plot the results on a graph against standard concentration on linear graph paper Refer to example shown in Figure 4 3 Issue 7 24 March 2012 ECN 736 25 Model 410 amp 410C Flame Photometer with Interface Operating Instructions continued 4 4 Operating Instructions continued DISPLAYED VALUE 30 20 28 19 10 15 29 SODIUM CONCENTRATION PPM Figure 4 3 Typical Calibration Curve 14 15 16 17 410 91 001 Check calibration standards and blank readings Dilute the unknown solutions with diluent to give a concentration of the element under test within the range of the calibration standards Several attempts might be necessary to determine the correct dilution ratio Aspirate each of the diluted unknowns for 20 seconds and then note the reading The concentration of the element in the unknown sample can be calculated by reading the sample concentration from the calibration curve and multiplying it by the dilution factor Recalibrate the instrument by carrying out paragraphs 8 to 12 Experience in u
7. which are inconsistent or in conflict with the terms of this warranty including but not limited to the limitations of the liability of Sherwood as set forth above shall not be binding upon Sherwood unless reduced to writing and approved by an officer of Sherwood Scientific Ltd Except for the obligations specifically set forth in this warranty statement in no event shall Sherwood be hable for any direct indirect special incidental or consequential damages whether based on contract tort or any other legal theory and whether advised of the possibility of such damages Neither Sherwood nor any of its third party suppliers makes any other warranty of any kind whether expressed or implied with respect to Sherwood Products Sherwood Scientific Ltd 1 The Paddocks Cherry Hinton Road Cambridge CB1 8DH England 410 91 001 Issue 7 24 March 2012 ECN 736 De
8. 1 Services Required Electrical Supply An a c supply at 100V 120V 220V or 240V 10 at 50 or 60Hz is required for the Model 410 The instrument operating voltage is shown on the voltage selector on the rear panel CAUTION f the voltage setting is not correct for the local supply do not attempt to use the instrument until it is correctly adjusted as detailed in Section 2 4 paragraph 11 Fuel The Model 410 can work with Butane Propane LPG and Natural Gas However for Calcium determination we would only recommend the use of Propane The use of Natural Gas 1s not advised for Calcium or any accurate work A supply of Propane Butane or Propane Butane mixture regulated at the cylinder to 2 1kg cm 30psig flow rate at least 0 4 litres per minute The use of industrial quality gas is not recommended as impurities can enter the delicate gas regulators and can leave deposits of oil and dirt which will render the instrument inoperable Natural gas at 3 to 10 inches water gauge regulated to 2 inches water gauge can also be used Natural gas and camping gas regulators are available as optional accessories refer to Section 8 2 NB The positioning of Gas cylinders should conform to National and local regulations Air A supply of clean dry oil free air at 1kg cm 14psig flow rate 6 litres per minute A suitable Sherwood Scientific air compressor is listed in Section 8 2 Waste Container A sink or waste container sited to the
9. Output Rev x xx Serial No xxxxx Checksum xxxxxx Normal Present value 1 slope xx xxx intercept x xxx Lineariser Present value 1 slope xxx xxx intercept x xxx Enter menu item number newline 1 Calibrate 2 Check Calibration 3 Set Clock not Bluetooth 4 orq to Quit Enter 3 and follow the instructions Enter date and time in format dd mm yy hh mm ss If fault persists contact your Sherwood Scientific Distributor 410 91 001 Issue 7 24 March 2012 ECN 736 44 Model 410 amp 410C Flame Photometer with Interface Spares and Accessories 8 1 Ordering Information When ordering spares or accessories for your instrument please give the following information to your Sherwood Scientific distributor Instrument Serial No Catalogue No of Part Cat No Description Quantity required This will ensure that your order is dealt with quickly and efficiently The number shown in the third column Quantity is the quantity of items that are supplied against the stated Catalog Number If the quantity is greater than 1 then only multiples of that quantity can be supplied 8 2 Spares and Accessories Serial Number Item Quantity 420 08 102 Nebuliser 1 410 26 001 Mixing chamber and burner 1 402 12 001 End Cap Assembly 1 400 02 013 Retainer End Cap 1 401 11 000 Constant Head and Drain 1 001 48 105 Fuse 200mA slo blo 1 400 22 003 Tube Nebuliser Inlet 1 400 20 001 Fuel restrictor 1 410 19 002 Natural gas regulator
10. at a minimum pressure of 1kg cm 14psig at 6 litres minute as supplied by a Model 851 Air Compressor Maximum inlet pressure 2 1kg cm 30psig If condensation problems arise a Model 855 Air Compressor should be used which has a water separator fitted Size Overall including chimney and rear panel connectors 510mm high x 390mm wide x 345mm deep Weight 9 5 kg 410 only 410 91 001 Issue 7 24 March 2012 ECN 736 du Model 410 amp 410C Flame Photometer with Interface 4 Operating Instructions 4 1 Front Panel Controls and Indicators See Fig 4 1 Power on item 10 LED which illuminates when the instrument 1s switched on Flame on item 11 LED which illuminates when the flame is alight Blank item 5 This control is used to set the display to zero while aspirating a blank solution Sensitivity Fine and Coarse item 7 amp 6 These two controls are used to set the display to an appropriate concentration reading while aspirating a standard solution The coarse control is a four position rotary switch and the fine control is a 10 turn potentiometer Fuel item 4 This control provides a fine adjustment of the fuel flow and enables the optimum flame conditions to be set for each element Na K Ca item 1 This three position slider control selects the correct filter for the element to be determined When used with other filter types e g Li or Ba the appropriate label should be fitted in the relevant
11. coloured sides of the filters are uppermost and the thumb grip is at the top as shown in Figure 6 2 CAUTION Handle the optical filters only by the edges never the faces Using a soft probe cotton bud or similar that will not scratch the filters push out the first filter Examine the filter edges and note the wavelength on Figure 6 2 alongside the appropriate space Remove the remaining two filters and mark the wavelengths on Figure 6 2 Carefully wipe each filter with a cotton bud soaked in methanol Turn the filter selector over so that the large holes and filter retainers are uppermost Refer to Figure 6 2 and carefully replace the filters mirrored sides upward into their respective positions Refit the filter selector into position inside the chimney and slide it fully in Check that all three positions can be selected Issue 7 24 March 2012 ECN 736 35 Model 410 amp 410C Flame Photometer with Interface Maintenance continued 6 8 Cleaning the Optical Filters and Glass Chimney continued STANDARD FILTER POSITIONS AS SUPPLIED Figure 6 2 Filter Selector 10 Soak the glass chimney in a 1 in 100 dilution of Cleaning Solution agitating it periodically 11 Rinse the glass chimney thoroughly in deionised water and dry with clean tissues 12 Replace the glass chimney with the clear strip at the bottom Make sure the glass chimney is seated correctly and refit the chimney cap on top of the chimney 410 91
12. right of the instrument will ensure the minimum length of waste tubing Do not use a waste container with high sides as this will cause the drain tube to be lifted above the level of the constant head drain 410 91 001 Issue 7 24 March 2012 ECN 736 sge Model 410 amp 410C Flame Photometer with Interface Installation continued 2 2 Site Conditions WARNING Under no circumstances install the instrument beneath overhanging 410 91 001 cupboards There must be at least 1 metre of clear space above the chimney For optimum performance this instrument should be installed in accordance with the following conditions The environment must be clean and free from dust The instrument must be placed on a strong level worktop free from vibration The Model 410 requires approximately 500mm x 500mm of bench space which includes an area in front for solutions and clearance at the rear for fuel and air tubing with clear access to the mains supply switch Avoid sites that expose the instrument to direct sunlight or draughts To meet the specification the ambient temperature must be within the range 10 C to 35 C and the maximum relative humidity must not be more than 85 non condensing Make sure that the correct voltage is selected for the local a c supply as shown in the following table Do not attempt to connect the power supply lead until the voltage is correctly set VOLTAGE SELECTED VOLTAGE RANGE 100V 90V to 110V 120V 100V
13. the flame photometer When the start up sequence has completed the module will be energized this is indicated by the illumination of the printer symbol and B for blanking The following will be displayed on the computer Flame Photometer Data Output Rev x xx Serial No xxxxx Operation and output are as above from this point onward Switching the Linearisation Function On and Off By selecting the Lineariser Sodium Na results will be corrected according to well understood principles so that all levels of Sodium concentration upto 20ppm fall within the linear range of the instrument and only one standard and zero value are required To switch the Lineariser function on press and hold the Select button while moving the Filter Selector to Na When the Select button is released the buzzer will stop and C will be illuminated The C will remain illuminated no matter what the position of the filter holder but the Lineariser only operates when Na is selected To switch off the Lineariser press and hold the Select button while moving the Filter Selector from Na to K When the Select button is released the buzzer will stop sounding and the C will no longer be illuminated For a Digital Interface prior to 21807 or M410 prior to 20018 it is necessary to remove the Digital Interface to switch the Lineariser on or off 410 91 001 Issue 7 24 March 2012 ECN 736 29 5 Model 410
14. 001 Issue 7 24 March 2012 ECN 736 36 Model 410 amp 410C Flame Photometer with Interface Maintenance continued 6 9 Voltage Selection and Fuse Replacement EQUIPMENT REQUIRED Fuses WARNING For continued protection against fire hazard use only the same type and 410 91 001 rating of fuse that was fitted originally to the M410 refer to instrument rear panel Check that the flame on LED is off fuel is turned off at source and the instrument and compressor are switched off Disconnect the a c supply lead from the a c supply socket Disconnect the a c supply lead from the power connector on the rear panel Open the voltage selector compartment and withdraw the voltage selector item 1 Figure 6 3 To replace fuses continue with paragraph 5 to change the operating voltage continue with paragraph 7 Slide out the two fuse holders item 2 Figure 6 3 and remove the fuses Replace any burned out fuses and slide in the fuse holders Replace the voltage selector so that the required voltage setting is visible through the window of the compartment cover Close the compartment cover and refit the a c supply lead Figure 6 3 Voltage Selector and Fuse 1 Voltage selector 2 Fuse holders Issue 7 24 March 2012 ECN 736 dt Model 410 amp 410C Flame Photometer with Interface Maintenance continued 6 10 Deproteinising or Disinfecting Procedure NOTE To deproteinise the system use Deproteinising Solution to disinfect
15. 39 UE OEA EE EA ETICA 39 Fuel Supply 39 i Unable to set display to 000 40 14 Unable to set display to standard reang secari ere er ere errr eee 40 0 Me EG PRORPIO A CETRA CORRE RE RC OT TTT TT inno 40 7 6 ici du i 4 L Poe LED P OE EAEE 42 7 8 nno 42 VR Date amp Time Not Reported LOT 43 ELA i gt EET T 43 CE LE L E RIE 44 e fe Bi ee e E eee rer er ae A er er re ee 45 8 1 B A a E A A 1 RR ATENE 45 8 2 Spares and ACCESSOLrIEeS vr 45 8 3 IIS BOTA ILA 0 PROT N n nn OT 46 Fe PRIORI I AR NE E ONES DIE ORE ONES ORE SAAIE NTTONOT 47 EE E a EEA JJ 47 Bee E E E ET E E SIA Ano 48 ace 48 LI AR 53 HyperTerminal 53 I RAEE 54 iui i III ere er ae rere seer rere ere 54 LOU ARR COTTA 56 410 91 001 Issue 7 24 March 2012 ECN 736 5 Model 410 amp 410C Flame Photometer with Interface Introduction 1 1 Introduction The Models 410 Industrial amp 410 Clinical are single channel low temperature flame photometers The Model 410 Industrial is supplied as standard for the determination of Sodium Na Potassium K and Calcium Ca The Model 410 Clinical is for the determination of Na K and Lithium Li in diluted clinical samples An automatic sample dilutor Model 805 is available This manual chiefly refers to the Model 410 Industrial but much is common to the Model 410 Clinical For Model 410 please assume the text applies to both models un
16. 8 bean 18 BE RR RR E OTT O 18 di i _ dd inn 18 RCA LOSCO 12 COU PRE 20 ec n f eee ee eee 20 ARR eT a 20 C8 FEE rrir 89t8t8t8t8t8 cc 20 area eget EA 20 nee eee ee A E E 20 e rr oo rr O n 20 Bs te ce ie ess te oe ato sv te ote res ne wn te cts A EARLE 20 Pl 20 O ge cctrict989898 23 ELE PREC 23 LL erro OOO 23 Claber 23 EGO RR A 23 4 3 LI i 24 4 4 Poe AEE aE I E E A E 25 4 5 ee aa 27 4 6 e it 27 4 7 c ca 28 4 8 Switching the Linearisation Function On and Off 29 OLEOLE 30 i el _ n 30 5 2 EL A RR A O A 30 MAE E TR TI RT I e i 31 6 1 ll 31 6 2 Io RA 31 6 3 idee SBOE Ge E en re rrr E erent nme ee ye er 31 6 4 Monthly Maintenance 31 6 5 Six Monthly Matitenian08 dalla 32 6 6 VE LG Bee E 32 voce A RTTARA 32 Cei ci 32 6 7 Cleaning the Mixing Chamber Burner and Drain 34 6 8 Rei The Optical DELI 35 6 9 Voltage Selection and Fuse Replacement ooi issesesssnsnsesesnsesnensnsesndeswondesesnteswantovesnteds 37 6 10 Deproteinising or Disinfecting Procedure cccccccsesecceececsesccessceesescessssenauaes 38 410 91 001 Issue 7 24 March 2012 ECN 736 sA Model 410 amp 410C Flame Photometer with Interface Contents Page ori 39 LA Power on LED not illuminated cccecceccsccscescncccenseesessessescescuscusesenses 39 T2 Flame on ii ln 39 or E E A E AA
17. Model 410 Industrial amp Model 410 Clinical Flame Photometer Operator Manual Copyright 2012 Sherwood Scientific Ltd All rights reserved 410 91 001 Issue 7 24 March 2012 ECN 736 G DOCUMENT CONTROL Manuals Flame 410 41091001 M410 amp 410C with Interface Operator Manual Issue 7 doc Model 410 amp 410C Flame Photometer with Interface Intended Use This operator s manual contains complete instructions for setting up and using the Model 410 Service information for use by appropriately qualified personnel is also available The Model 410 is intended for use by persons knowledgeable in safe laboratory practices If the instrument is not used in accordance with these instructions for use the protection provided by the equipment may be impaired WARNING The Model 410 is designed to be grounded through the power supply lead line cord for safe operation For the safety of operating personnel and optimum performance make sure that the instrument is only connected to a 3 prong socket outlet that has an effective earth connection If you are in any doubt about the safety of your electrical supply system consult a competent qualified electrician There are no user replaceable parts within the instrument Do not remove the rear cover from the instrument Sherwood Scientific Limited and its authorized Distributors and Agents consider themselves responsible for the effects of safety reliability and performance of the Model 410 onl
18. al terminal N If necessary set the power switch below the chimney to the 0 position Connect the a c supply lead to the power receptacle on the rear panel and connect the plug to a convenient supply socket If a printer or computer is to be used with the instrument continue with Section 2 5 If a chart recorder is to be used with the instrument refer to Section 2 6 If neither is to be used continue with Section 4 Operating Instructions 2 5 Connecting a Printer or Computer l Connect the round 4 pin plug of the Printer Computer Lead 410 09 003 to the socket on the upper left of the front panel Connect the 9 way D plug to a serial printer a 9 to 25 way adapter may be required this is supplied as standard with a Sherwood serial printer or to the serial port of a computer If your computer does not have a serial port use a USB to serial adapter these are readily available from your local computer store Continue with Section 4 Operating Instructions 2 6 Connecting a Chart Recorder l 410 91 001 Connect a black plug Cat No 001 42 017 to the black ve recorder input lead and a red plug Cat No 001 42 254 to the red ve input lead Fit the plugs into the appropriate data output sockets on the rear panel of the instrument If the chart recorder has an input shorting link fitted connect it between the black negative input and the earth input This may reduce interference and produce a better t
19. d Ca x 100ml x6 a Fitting a Lineariser Refer to figure 2 7 attached The lineariser will normally arrive already installed in your instrument provided you have ordered a Model 410 Clinical in which case you can ignore this section al Identify the Lineariser which may be packed separately all Remove the Logo Plate from the front of the instrument by carefully prising off one end of the plate with a flat bladed screwdriver ai Check the alignment of the Lineariser Module pins with the 9 sockets on the main printed circuit board which is revealed behind the logo plate Gently but firmly push home the pins 410 91 001 Issue 7 24 March 2012 ECN 736 48 Model 410 amp 410C Flame Photometer with Interface Appendix B continued Figure 2 7 1 Logo Plate 2 Lineariser Module 3 2 Measurement Ranges for clinical samples Na serum 110 170mmol I diluted 1 200 12 6 19 5ppm aspirated into flame Na Urine 0 200mmol I diluted 1 200 0 to 23ppm aspirated into flame K serum 0 10 0mmol 1 K urine 0 200mmol I Li serum 0 3 0mmol l These are concentrations in the original samples before dilution For 120 200mmol I1 samples must be pre diluted 1 in 5 prior to 1 in 200 dilution 410 91 001 Issue 7 24 March 2012 ECN 736 49 Model 410 amp 410C Flame Photometer with Interface Appendix B continued 3 3 Specificity Na Range Errors in the Na readings due to Ca K Li presence in biological samples sha
20. dule right hand button whilst switching on the flame photometer When the start up sequence has completed release the button The buzzer will sound the printer symbol will be flashing B will be illuminated and the following will be printed Sherwood Printer option Vx xx Clock set as 02 Feb 06 15 00 29 Press the Print button to increment the minutes To decrement the minutes press the Select button to illuminate C then press the Print button Press the Select button to illuminate S now when the Print button is pressed the hours will increment To decrement the hours press the Select button to illuminate R then press the Print button To exit this mode wait 30 seconds and the instrument will revert to normal operation with the B and Printer symbol illuminated The printer will print Sherwood Printer option Vx xx 410 91 001 Issue 7 24 March 2012 ECN 736 43 Model 410 amp 410C Flame Photometer with Interface Troubleshooting continued 7 10 Date amp Time Not Reported Correctly continued With A Computer Connect the module to a PC serial port then access HyperTerminal and use these settings 9600 baud 8 bit No parity Stop bit 1 Flow Control None See Appendix C for details on accessing HyperTerminal Setting the Date amp Time Type in to HyperTerminal and the following menu will appear Flame Photometer Data
21. e select button will cause the buzzer to sound and the next LED in the sequence B C S R to illuminate Print Button item 3 Each press of the print button will cause the buzzer to sound and the interface to output to a printer or computer M410 C item 4 LED which illuminates when the lineariser is activated on the interface module B item 5 When B is illuminated pressing the print button will print out the displayed value with the word blank and the date and time C item 6 When C is illuminated pressing the print button will print out the displayed value with the word calibration and the date and time S item 7 When S is illuminated pressing the print button will print out the displayed value with the word sample and the date and time R item 8 When R is illuminated pressing the print button will print out the displayed value with the word repeat and the date and time Print item 9 This LED 1s constantly illuminated If the print button is pressed without a printer connected the LED will flash 410 91 001 Issue 7 24 March 2012 ECN 736 20 Model 410 amp 410C Flame Photometer with Interface Operating Instructions continued Front Panel Controls and Indicators continued 4 1 M410 C Flame Photometer P Sherwood i af NE Me af Figure 4 1 1 Interface Panel 1 Printer Computer connector 2 Selector button 3 Print button 4 Lineariser LED 5 Blank LED 6 Calibration LED 7 Sam
22. ear If the suggested remedies in detailed in Section 7 6 have not cured the problem then recalibrate the lineariser module as detailed below Lineariser On Ensure the element select switch on the flame photometer is set to Na and then connect the module to a PC running HyperTerminal with the lead provided and switch on the flame photometer It is not necessary for gas and air to be connected Enter and then in to HyperTerminal to enter the calibration mode the following message is returned Calibrate Lineariser Present value l slope xx xxx intercept x xxx Enter reading newline when DPM stable or q to abort When the number on the instrument display has stabilised type it into HyperTerminal and press return A total of 4 numbers will be presented after the last one is entered new values for slope and intercept will be displayed in HyperTerminal and the menu will reappear Enter q to exit set up mode If fault persists contact your Sherwood Scientific Distributor Spares and Accessories Serial Number Description 47541100 Model 805 Automatic Dilutor 86000009 Model 860 Autosampler 41086001 Digital Interface Lineariser Module Solutions Clinical Solutions 00156100 Bottle containing 100ml of following concentrations Na 140mmol I K 5 0mmol l Ca 0 0mmol l Li 1 5Smmol l 00156183 Deproteinising Solution pack 6 x 80ml 410 91 001 Issue 7 24 March 2012 ECN 736 52 Model 410 amp 410C Flame Photometer with In
23. ed on a digital readout 1 4 Reagents Sherwood Scientific supplies a wide range of reagents including standards diluent and maintenance solutions for use with the Model 410 Flame Photometer Please refer to Section 8 3 and Appendix B for a complete list of the reagents Dilutions Samples and standards must be diluted with the same batch of diluent made up of 1 part Diluent Concentrate to 999 parts deionised or good quality distilled water The same batch of diluent should be used to zero the instrument and to prepare dilutions of standards and samples This will prevent variations in water purity affecting the measurements Great care should be taken so that contamination does not occur when preparing the samples and standards Remember that the accuracy of the instrument is dependent on the accuracy and purity of the standards used for calibration Storage All solutions should be stored away from direct sunlight in a cool place below 25 C 77 F in an airtight container to prevent evaporation and discolouration Glass containers should not be used as they can affect Na concentration levels Prolonged exposure to the atmosphere must be avoided to prevent evaporation of standard solutions which could affect concentration Purification No purification is required for Sherwood Scientific standard solutions 410 91 001 Issue 7 24 March 2012 ECN 736 sa Model 410 amp 410C Flame Photometer with Interface Installation 2
24. ed to purge the tube of air Allow excess water to flow back into the drain IMPORTANT Do not continue until you are satisfied that the U tube has been completely filled with water and is purged of air The M410 Industrial is supplied with Na K and Ca filters fitted To replace any of these filters refer to Section 6 8 Figure 2 5 Voltage Selector 1 Voltage selector 2 Fuse holders Check that the voltage shown on the voltage selector is correct for the local supply If it is not open the voltage selector compartment Slide the voltage selector bobbin item 1 Figure 2 5 out of the instrument and replace it so that the required voltage setting will be visible through the window of the compartment cover Close the compartment cover CAUTION Do not attempt to rotate the voltage selector bobbin when it is fitted in the voltage selector as this will damage the contacts Issue 7 24 March 2012 ECN 736 e Model 410 amp 410C Flame Photometer with Interface Installation continued 2 4 Assembly continued 12 13 14 Fit a suitable 3 pin plug to the a c supply lead Check that the correct colour coded leads are connected to the plug terminals Follow the plug manufacturer s fitting instructions CAUTION Ensure that the green yellow earth connector in the a c supply lead is connected to a properly grounded earth point the brown lead is connected to the Live terminal L and blue lead is connected to the Neutr
25. ess sliding the Filter Selector to Na while holding down the Select button While waiting for the flame to warm up prepare diluted standard and solutions prepared in accordance with the dilutions described in e above With the Lineariser fitted it is only necessary to prepare a single standard at the expected sample concentration level Set the zero control to show 0 on the display while aspirating the blank solution containing other elements if measuring Lithium see notes e above Set the display to the value of the undiluted standard e g 140mmol l Na while aspirating the diluted standard Repeat until values are obtained within 1 Aspirate sample and note reading on the display which will be the value of the concentration of the undiluted sample Shutdown l Aspirate Deproteinising solution diluted 1 100 with deionised water for 1 minute 2 Then aspirate diluent solution for two minutes 410 91 001 Issue 7 24 March 2012 ECN 736 51 Model 410 amp 410C Flame Photometer with Interface Appendix B continued 5 2 7 10 Hazards WARNING When the Model 410 is used in a pathology laboratory cleaning of component parts such as the nebuliser mixing chamber burner parts drain tubing and vessels should be carried out very carefully using protective clothing gloves and eye guards Refer to section 6 10 All items should be treated as though they contain pathogenic organisms Sodium Results Non Lin
26. g Soak the nebuliser in a 1 in 100 dilution of Cleaning Solution agitating it periodically Rinse thoroughly in deionised water and shake dry Refit the nebuliser inlet tubing and the air line Recheck aspiration rate If the nebuliser operation is still unsatisfactory fit a new nebuliser Do not attempt to adjust the nebuliser as the capillary position is fixed during manufacture and is not adjustable Issue 7 24 March 2012 ECN 736 332 Model 410 amp 410C Flame Photometer with Interface Maintenance continued 6 7 Cleaning the Mixing Chamber Burner and Drain EQUIPMENT REQUIRED Flat blade screwdriver blade width 8 mm Deproteinising Solution Tissues l 10 11 2 13 14 15 16 17 18 19 20 410 91 001 Check that the flame on LED is off fuel is turned off at source and the instrument and compressor are switched off Release the nebuliser retainer and withdraw the nebuliser items 1 and 2 Figure 6 1 Disconnect the fuel tubing item 4 Figure 6 1 from the end cap connector item 3 Figure 6 1 using a twisting movement Do not pull the tubing as this will make it grip the connector more tightly WARNING Use a heat resistant glove when handling the burner and burner tube if the flame has been alight within the preceding 30 minutes Do not proceed until all the parts within the chimney are at a safe handling temperature Remove the chimney cover and lift out the glass chimney
27. g Wire into the Nebuliser Inlet Tube and pull right through Inspect the Nebuliser Inlet Tube replace if signs of wear or accumulation of particles are apparent With the Nebuliser Inlet Tube removed but with the Nebuliser attached to the Air Tubing place a finger over the Nebuliser Outlet Nozzle turn on the air supply to force any residue out of the Nebuliser s capillary tube Repeat steps 7 8 and 9 until the aspiration rate is constant and between 3 and 6ml per minute Replace the Nebuliser and rinse with distilled water Note Before despatch each nebuliser is adjusted for optimum performance This adjustment is critical and under no circumstances should you attempt to readjust or dismantle the assembly If adjustments are made to the atomiser we shall not accept responsibility for poor performance from your Flame Photometer 410 91 001 Issue 7 24 March 2012 ECN 736 590 Model 410 amp 410C Flame Photometer with Interface Maintenance continued Cleaning the Nebuliser continued 12 13 14 bo 16 17 410 91 001 Figure 6 1 Mixing Chamber 1 Nebuliser retainer 2 Nebuliser 3 End cap 4 Fuel tubing 5 Air tubing 6 End cap securing screw 7 U tube Fit a new length of nebuliser inlet tubing to the nebuliser Recheck the aspiration rate paragraphs 3 4 and 5 If nebuliser operation is still outside the specification unscrew the air line connector and remove the nebuliser inlet tubin
28. grees to lock it in position Refit the glass inner chimney and the chimney cover Fit the constant head drain to the spring clip and position it so that the lip on the drain is resting on the spring clip Connect the fuel tubing to the mixing chamber end cap Connect the U tube between the mixing chamber end cap and the constant head drain Fit the drain tube to the constant head drain Fit the nebuliser into the end cap and position the retainer to lock it Use a wash bottle to fill the U tube with deionised water Sufficient water should be used to completely fill the U tube and purge it of air Issue 7 24 March 2012 ECN 736 34 lt Model 410 amp 410C Flame Photometer with Interface Maintenance continued 6 8 Cleaning the Optical Filters and Glass Chimney EQUIPMENT REQUIRED Soft lint free cloth Methanol Cotton buds Tissues Cleaning Solution l 410 91 001 Check that the flame on LED is off fuel is turned off at source and the 410 and compressor are switched off WARNING Do not proceed until all the parts within the chimney are at a safe handling temperature Lift off the chimney cap from the chimney assembly Lift out the glass chimney Move the filter selector to the top position and push the lever upwards and to the right This will disengage the filter selector from the stop Lift the filter selector out of the chimney Position the filter selector on the lint free cloth so that the
29. hazardous With the exception of the glass chimney there are no user maintainable parts inside the Model 410 covers Never remove covers from the instrument unless specific maintenance instructions are being followed Propane butane and natural gas are highly inflammable and potentially explosive gases Propane and butane are stored as a liquid under pressure in a cylinder for use with the Model 410 Such a cylinder should never be subjected to heat or mechanical shock When handled correctly and connected to the instrument as instructed the fuel gas is quite safe Check hosing joints with a soap solution before allowing any naked flame in the vicinity Never open a cylinder valve to atmosphere even on a supposedly empty cylinder The chimney cap and glass chimney and the area above the chimney can become very hot and are capable of causing severe burns Never view the flame from the top of the chimney always use the inspection hole Make sure that the air compressor is connected to the power supply and switched on before starting a flame ignition sequence Failure to observe this precaution may result in a build up of fuel gas which will cause a flame to appear above the chimney Issue 7 24 March 2012 ECN 736 30 6 1 6 2 6 3 6 4 Model 410 amp 410C Flame Photometer with Interface Maintenance General Under the Daily Weekly Monthly and Six Monthly Maintenance headings are summaries of the work and equipment requ
30. herwood Scientific Limited Product Warranty Statement Warranty Term 12 Months Sherwood Scientific Ltd Sherwood warrants subject to the conditions itemised within this document through either Sherwood personnel or personnel of its authorised distributors to repair or replace free of all charges including labour any part of this product which fails within the warranty time specified above appertaining to this particular product Such failure must have occurred because of a defect in material or workmanship and not have occurred as a result of operation of the product other than in accordance with procedures described in the instructions furnished with this product Conditions and specific exceptions that apply to the above statement are as follows l End user warranty time commences on the date of the delivery of product to end user premises Free of all charges statement applies only in areas recognised by Sherwood as being serviced either directly by its own personnel or indirectly through personnel of an authorised distributor Products purchased outside these areas requiring service during the warranty period will incur charges relative to the travel transit costs involved However products purchased in such areas will be serviced during the warranty period free of all charges providing they are returned carriage paid to either Sherwood or by pre arrangement to an authorised Sherwood distributor All maintenance other
31. ion 6 2 Troubleshooting When the flame on LED illuminates proceed with Section 4 4 Operating Instructions paragraph 4 Issue 7 24 March 2012 ECN 736 Die Model 410 amp 410C Flame Photometer with Interface Operating Instructions continued 4 4 Operating Instructions NOTE For greatest accuracy and stability Sherwood Scientific recommend that a batch of diluent is made up from part Diluent Concentrate and 999 parts deionised or good quality distilled water Using diluent for setting blank and for dilution of samples and standards means that all solutions presented to the instrument contain the stability promoting ingredients contained in the diluent Always use the same batch of diluent for the blank and the dilution of samples and standards Store the diluent in a sealed container for not more than five days WARNING Always check that the gas installation is leakproof before initiating an 10 11 12 13 410 91 001 ignition cycle Turn on the fuel supply at source Switch on the Air Compressor Depress the power switch to switch on the instrument The power on LED will be illuminated and an ignition cycle will commence If the flame on LED is not illuminated at the end of the ignition cycle refer to Section 4 3 and check the setting of the fuel control Set the filter selector to the required position Insert the nebuliser inlet tube in a beaker containing 100ml of diluent and allow 30 minutes for the ope
32. ir tubing If this problem recurs frequently use a Model 855 air compressor with water separator Make sure the air compressor filter is changed regularly refer air compressor manual If the atmosphere surrounding the flame photometer or air compressor inlet is contaminated e g smoke unstable readings may result A similar effect may be observed if the instrument is sited in a draught If fault persists contact your Sherwood Scientific Distributor 410 91 001 Issue 7 24 March 2012 ECN 736 40 Model 410 amp 410C Flame Photometer with Interface Troubleshooting continued 7 6 Non linear results Check the concentration of the solutions being aspirated The solutions should all contain in 1000 dilution of Diluent Concentrate refer Section 4 4 Make up fresh solutions and recheck calibration curve Check that the fuel supply is adequate Make sure the fuel control is set correctly refer Section 4 3 The nebuliser may be blocked Check the nebuliser refer Section 6 6 The U tube or drain tubing may be blocked or partially obstructed Use fresh deionised water and recalibrate zero If using low grade deionised water the background levels may be too high For M410 Industrial only refer to Appendix B section 7 10 for M410 Clinical Connect the module to a PC serial port then access HyperTerminal and use these settings 9600 baud 8 bit No parity Stop bit 1 Flow Control None See Appendix C for detai
33. ired The tasks are detailed from Section 6 6 onwards For maintenance of the air compressor dilutor and chart recorder if used refer to the instructions supplied with the equipment Daily Maintenance EQUIPMENT REQUIRED None l Empty waste container 1f used 2 Check air line for condensation and drain if necessary 3 Check U tube is filled with deionised water 4 Rinse nebuliser with distilled water after use Weekly Maintenance EQUIPMENT REQUIRED 10 ml beaker Stop watch Nebuliser cleaning wire Deproteinising Solution Nebuliser tube and sleeve or polythene inlet tube as required l Carry out Daily Maintenance procedure 2 Deproteinising the system Section 6 10 3 Check the operation of the nebuliser Section 6 6 Monthly Maintenance EQUIPMENT REQUIRED As for weekly maintenance l Carry out Daily and Weekly Maintenance procedures 2 Check the constant head drain mixing chamber U tube and drain tube and clean if necessary Section 6 7 Check that the U tube is refilled with deionised water on reassembly 410 91 001 Issue 7 24 March 2012 ECN 736 531 Model 410 amp 410C Flame Photometer with Interface Maintenance continued 6 5 Six Monthly Maintenance EQUIPMENT REQUIRED As for weekly maintenance plus the following U tube and drain tube Methanol tissues Soft lint free cloth Cotton buds Carry out the Daily and Weekly Maintenance procedures Clean the
34. is an adequate supply of fuel Fuel supply must be turned on at source If using a long length of fuel tubing try repeating the ignition sequence a few times to allow the fuel to fill the tubing If using natural gas check the setting of the natural gas regulator refer to Section 4 3 If the fuel is propane or butane check that the regulator at the cylinder is adjusted for 2 1kg cm 30psig Check the fuel control is set correctly refer to Section 4 3 If the fuel restrictor propane butane only 1s blocked clean with the nebuliser cleaning wire refer to Figure 2 1 If fault persists contact your Sherwood Scientific Distributor 410 91 001 Issue 7 24 March 2012 ECN 736 39 Model 410 amp 410C Flame Photometer with Interface Troubleshooting continued 7 3 7 4 7 9 Unable to set display to zero Check that diluent is being aspirated The deionised water supply may be contaminated use a better grade of deionised water The burner may be encrusted with salt deposits Clean the burner as detailed in Section 6 7 If using a chart recorder check that the data output connections are correct If fault persists contact your Sherwood Scientific Distributor Unable to set display to standard reading Check the concentration of the standard being aspirated and that the dilution ratio of this standard is correct Use fresh deionised water and recalibrate zero If using low grade deionised water the background levels ma
35. itable container Volume The maximum diluted sample volume required for 20 seconds aspiration is 2ml Interface Output RS232 via cable 410 09 003 Pin 2 Rxd Pin 3 Txd Pin 5 Gnd Chart Recorder Output Nominal 100mV signal output per 1000 display units Environmental Conditions Temperature Operating 10 C to 35 C Transportation 40 C to 45 C Humidity Operating 85 maximum at 35 C Transportation 95 maximum at 45 C non condensing The instrument specification will be unaffected by an ambient temperature change of 4 C or less per hour within the range 10 C to 35 C with a maximum of 7 C shift during 8 hours Installation Category Installation Category 1 410 91 001 Issue 7 24 March 2012 ECN 736 16 Model 410 amp 410C Flame Photometer with Interface Performance Characteristics and Specification continued 3 10 3 11 3 12 3 13 3 14 Power Requirements Voltage 90V to 132V or 198V to 264V 50 60Hz Fuses Two 200mA slo blo fuses are fitted for all voltage ranges Power 20VA Fuel High grade Propane Butane or Propane Butane mixture All fuels to be free of heavy hydrocarbon deposits and regulated at the cylinder to approximately 2 1kg cm 30psig Natural Gas at 3 to 10 inches water gauge regulated to 2 inches water gauge using optional natural gas regulator NOTE Natural Gas may give reproducibility results outside specification Air A supply of clean air
36. kit with filter 1 001 26 033 Sample pot plastic in packs of 50 1 pack 989 05 137 Sodium filter 589 6nm 1 989 05 138 Potassium filter 768nm 1 989 05 139 Lithium filter 671nm 1 400 16 004 Calcium filter 620nm with label 1 400 16 005 Barium filter 520nm with label 1 410 27 001 Caesium in Holder 1 410 27 002 Rubidium in Holder 1 410 27 003 Strontium in Holder 1 410 21 001 Filter holder recommended for use with Ca and Ba filters 1 410 09 003 Lead M410 to Printer Computer 1 410 91 001 Issue 7 24 March 2012 ECN 736 45 Model 410 amp 410C Flame Photometer with Interface Spares and Accessories continued 8 3 410 91 001 Serial Number 001 08 234 001 08 439 001 08 732 001 72114 001 72 116 001 08 718 100 99 010 851 01 001 851 01 000 855 01 001 855 01 000 410 89 001 475 41 100 471 71 900 001 26 074 001 26 033 001 92 492 ITEM Propane primary regulator cylinder fixing Butane primary regulator 182H for 32Ib Calor Caravan cylinder QUANTITY 1 1 Butane primary regulator for Gaz type cylinder 1 Fuel tubing Air tubing Unex tubing clamp Nebuliser cleaning wire pack of 3 Model 851 Air Compressor 230V Model 851 Air Compressor 115V Model 855 Air Compressor 230V complete with water separator Model 855 Air Compressor 115V complete with water separator service Manual Model 805 Dilutor Mains Supply Lead Disposable sample cups 1 5ml pack of 1000 Disposable sample cu
37. less specifically indicated that the information is relevant to the Model 410 Industrial only Information specific to the Model 410 Clinical not covered in the main body of the manual is described in Appendix B Additional filters are available to allow the determination of Barium Ba Cesium Cs Rubidium Rb and Strontium Sr Information regarding Ba Cs Rb and Sr determinations is available from your Sherwood Scientific distributor The Model 410 has a fail safe device which automatically stops the gas flow if the flame does not ignite or if during operation the flame is extinguished Plus an air pressure switch so that if the air pressure falls below a specified value the flame will not ignite or will be extinguished 1 2 Summary of the Test NOTE References are listed in Appendix A In many applications rapid availability of results is of prime importance By flame photometry both Sodium and Potassium results on a single sample can be available in less than 5 minutes of the sample reaching the laboratory The same is true of Lithium results a determination that was never carried out before the advent of flame photometry A simple dilution step is all that is required therefore sample handling losses and inaccuracies are at a minimum Ref 3 10 Prior to the advent of flame photometry Sodium and Potassium were typically determined gravimetrically after the precipitation of relatively insoluble salts such as Sodium Uranyl Zi
38. ll not be greater than Immol l where the levels of those elements do not exceed 3 5mmol l Ca 100mmol I K and 2 0mmol I Li K Range Errors in the K readings due to Ca Na Li presence in biological samples shall not be greater than Immol l where the levels of those elements do not exceed 3 5mmol l Ca 180mmol 1 Na and 2 0mmol I Li Li Range Errors in the Li readings due to Ca Na and K presence in biological samples shall not be greater than Immol l where the levels of those elements do not exceed 3 5mmol l Ca 180mmol I Na and 8 0mmol I K 3 4 Accuracy Linearity Na serum 2mmol l 0 2ppm Na Urine 4mmol l 0 4ppm K serum 0 2mmol l K urine 2mmol l Li serum 0 2mmol l The linearity specification is subject to 1 significant digit for display resolution and may not be achieved for results outside the measurement ranges specified above Drift Na serum urine better than 4mmol l K serum better than 0 2mmol l K urine better than 4mmol l Li serum better than 0 1mmol l These can be obtained measured over 5 minutes based on a run of 10 consecutive samples aspirated for 20 seconds then 10 seconds of air They are valid up to the maximum measurement range of the instrument Reproducibility Na 140mmol I better than 1 5 CV K 5 0mmol I better than 1 5 CV K 0mmol 1 better than 1 5 CV Li 2 5mmol l better than 2 5 CV Results obtained from 20 replicates of the same sample aspirated for 20 seconds then 10 seconds of air
39. ls on accessing HyperTerminal Ensure the element select switch on the flame photometer is set to K Ca or Li connect the module to a PC running HyperTerminal with the lead provided and switch on the flame photometer It is not necessary for gas and air to be connected Enter and then 1 into HyperTerminal to enter the calibration mode the following message is returned Calibrate Normal current I slope xx xxx intercept x xxx Enter reading newline when DPM stable or q to abort When the number on the instrument display has stablised type it into HyperTerminal and press return A total of 10 numbers will be presented after the last one is entered new values for slope and intercept will be displayed in HyperTerminal and the menu will reappear Enter q to exit set up mode If fault persists contact your Sherwood Scientific Distributor 410 91 001 Issue 7 24 March 2012 ECN 736 s ie Model 410 amp 410C Flame Photometer with Interface Troubleshooting continued Vf 7 8 Printer LED Flashing Printer or Computer not switched on or connected Both the Select and Print switch are inoperative when the Printer LED is flashing Make sure the Printer or Computer is switched on and the interface cable is securely connected and then switch the instrument off and on again If fault persists contact your Sherwood Scientific Distributor Output Does Not Equal Display Perform Calibration as detailed below Ensure the ele
40. m 4 14 inch connector for the air inlet tubing to the instrument from the air compressor outlet See Section 3 11 for pressure and flow rate specifications Air regulator item 5 Control to adjust the pressure of the air supply The air pressure gauge provides a visual indication of the air pressure 410 91 001 Issue 7 24 March 2012 ECN 736 roge Model 410 amp 410C Flame Photometer with Interface Operating Instructions continued 4 3 Initial Adjustment NOTE The following instructions assume that the air compressor is switched on and l 10 11 12 TS 14 410 91 001 supplying the instrument with air Check that the air pressure gauge on the rear panel indicates a reading between 11 and 13psig If it does not lower the air regulator locking ring and adjust the regulator for a reading of 1lpsig on the air pressure gauge Raise the locking ring to lock the air regulator adjuster WARNING The air compressor should always be switched on before the flame is ignited The gas will not flow without sufficient air pressure Check the Gas Trap U tube is filled with water refer to Section 2 4 paragraph 9 Turn the fuel control fully clockwise to the closed position but do not force it This control is a precision assembly that will be damaged by rough handling WARNING Always check that the gas installation is leak proof before initiating an ignition cycle Open the fuel control the required number of tur
41. ment select switch on the flame photometer is set to K Ca or Li connect the module to a PC running HyperTerminal with the lead provided and switch on the flame photometer Use these settings 9600 baud 8 bit No parity Stop bit 1 Flow Control None See Appendix C for details on accessing HyperTerminal Enter and then 1 into HyperTerminal to enter the calibration mode the following message is returned It is not necessary for gas and air to be connected Calibrate Normal current I slope xx xxx intercept x xxx Enter reading newline when DPM stable or q to abort When the number on the instrument display has stablised type it into HyperTerminal and press return A total of 10 numbers will be presented after the last one is entered new values for slope and intercept will be displayed in HyperTerminal and the menu will reappear Enter q to exit set up mode There may always be a difference of 1 for high values If fault persists contact your Sherwood Scientific Distributor 410 91 001 Issue 7 24 March 2012 ECN 736 sedia Model 410 amp 410C Flame Photometer with Interface Troubleshooting continued 7 9 Date amp Time Not Reported Correctly Set clock as detailed below With A Printer Connect the printer to the instrument with the cable provided and switch on the printer Make sure that the printer is set to 9600 baud 8 bit No parity Stop bit 1 Press and hold down the Print button on the mo
42. mixing chamber burner tube burner and constant head drain Section 6 7 Replace nebuliser tube U tube and drain tube Clean the optical filters and the glass chimney Section 6 8 Check the air and fuel tubing and connectors for leaks using soap solution Check for signs of stress cracking especially at the connectors 6 6 Nebuliser EQUIPMENT REQUIRED Stop watch 10 ml beaker Cleaning wire Cleaning Solution Stainless steel nebuliser tube and sleeve or polythene inlet tube as required Operational Check l Di ji 4 Turn off the fuel supply at source Switch on the M410 and the air compressor Check that the flame on LED 1s off Fill the beaker with deionised water and weigh it Present the 10ml beaker of deionised water to the nebuliser tube for an accurately timed minute Reweigh the beaker and calculate the aspiration rate If it is between 3 and 6 ml minute no further action is required If it is too low continue with paragraph 6 If it is too high contact your Sherwood Scientific Distributor Do not attempt to adjust the nebuliser as the capillary position is fixed during manufacture and is not adjustable Cleaning the Nebuliser 6 10 11 Release the nebuliser retainer item 1 Figure 6 1 and withdraw the nebuliser item 2 Figure 6 1 Remove the Nebuliser Inlet Tube Insert a Cleaning Wire into the capillary tube at the rear of the Nebuliser and pull right through Insert the Cleanin
43. nc Acetate Ref 1 and Potassium Chloroplatinate Ref 2 As with all chemical methods for these two elements there were cross interferences and also interference from other ions such as NH4 Many analytical steps such as protein precipitation or ashing of the sample were involved with all the attendant losses and inaccuracies and the complete procedures required many hours 410 91 001 Issue 7 24 March 2012 ECN 736 6 Model 410 amp 410C Flame Photometer with Interface Introduction continued 1 3 Principles of Operation When a solution is aspirated into a low temperature flame in an aerosol each droplet of water evaporates leaving a solid core of the residue of evaporation The core further breaks down to the molecular level and provided the molecules are not too refractory progress to form atomic species The atom then is excited by the flame and its electron temporarily moves to a higher energy state When the electron returns to the ground state it loses the excitation energy and a discrete wavelength of light 1s emitted characteristic of the atom The emitted light can be isolated from other wavelengths by an optical filter The amount of light being emitted is proportional to the number of atoms in the flame and it follows the concentration of that atom in the original solution The amount of light emitted can be measured by a suitable photodetector The photodetector generates an electrical signal which is amplified and display
44. ns counter clockwise as follows Fuel No of Turns Propane 9 Butane 11 Natural Gas 16 Turn on the fuel supply at source Depress the power switch on the front panel The power on LED will illuminate and an ignition cycle will commence If the flame on LED has not illuminated before the end of the ignition cycle switch off and wait ten seconds Then without adjusting the fuel control in case the instrument fuel system is not yet completely filled switch on again to initiate another ignition cycle Repeat this cycle twice to allow sufficient time for the fuel to reach the burner then continue to paragraph 9 If the flame on LED has not illuminated before the end of the ignition cycle switch off the power switch If using Natural Gas continue with paragraph 10 If using Propane Butane continue with paragraph 11 Remove the natural gas regulator screw cap item 1 Figure 2 3 Rotate the screw head fully clockwise and then counterclockwise exactly one and a half turns Refit the screw cap WARNING Do not exceed this setting as this will lead to a very rich fuel mixture which may cause a flame to appear above the chimney Open the fuel control one turn counterclockwise Switch on again If the flame on LED still does not illuminate repeat paragraph 11 Do not open the fuel control more than a total of 4 turns from the settings shown in paragraph 3 If the flame on LED still does not illuminate switch off and refer to Sect
45. ple LED 8 Repeat LED 9 Print LED ECN 736 24 March 2012 410 91 001 Issue 7 DI Model 410 amp 410C Flame Photometer with Interface Operating Instructions continued 4 2 Rear Panel Controls and Connectors Figure 4 2 Rear Panel 1 Power connector and voltage selector 2 Gas inlet connector 3 Data outout sockets 4 Air inlet connector 5 Air pressure gauge and air regulator adjuster 6 Propane Butane regulator inlet connector 410 91 001 Issue 7 24 March 2012 ECN 736 yee Model 410 amp 410C Flame Photometer with Interface Operating Instructions continued 4 2 Rear Panel Controls and Connectors See Fig 4 2 continued A IEC symbol advising user to refer to accompanying documentation Serial Plate symbol denotes equipment suitable for alternating current only Power item 1 Three pin connector for the a c supply lead incorporating a voltage selector and fuse compartment Data output item 3 Two colour coded chart recorder sockets The nominal 100mV 1000 display digits output signal is on the red socket with respect to the black socket Gas item 6 14 inch fuel inlet connector to the instrument permanently connected to the cylinder regulator outlet The instrument will operate satisfactorily on Propane and Butane or Natural Gas using the natural gas regulator available as an optional accessory refer to Section 8 2 See Section 3 10 for pressure and flow rate specifications Air ite
46. position on the front of the chimney assembly Decimal item 8 This touch control located adjacent to the digital display 1s used to select the decimal point e g using the decimal pushbutton to select the decimal point a reading of 1000 will be displayed as 100 0 The sensitivity of the Model 410 is unaffected by use of this control Power 0 1 item 9 Rocker switch that switches the a c power supply on 1 and off 0 When the instrument is switched on the power on LED 1s illuminated and an ignition cycle is initiated When switched off the flame is extinguished and the a c supply to the instrument is switched off 410 91 001 Issue 7 24 March 2012 ECN 736 105 Model 410 amp 410C Flame Photometer with Interface Operating Instructions continued 4 1 Front Panel Controls and Indicators continued Figure 4 1 Front Panel 1 Filter selector 2 Digital display 3 Inspection flap 4 Fuel adjustment control 5 Blank control 6 Coarse sensitivity control 7 Fine sensitivity control 8 Decimal pushbutton 9 Power switch 10 Power on LED 11 Flame on LED 410 91 001 Issue 7 24 March 2012 ECN 736 a Ore Model 410 amp 410C Flame Photometer with Interface Operating Instructions continued 4 1 Front Panel Controls and Indicators See Fig 4 1 1 continued Printer Computer Connector item 1 Four pin socket for connecting the instrument to a serial printer or computer Selector Button item 2 Each press of th
47. ps 25ml pack of 50 Guide to Flame Photometry Standard Solutions NOTE The following solutions are available Serial Number 001 56 620 001 56 621 001 56 622 001 56 623 001 56 184 001 56 681 001 56 682 001 56 183 Issue 7 ITEM Flame Photometer Standard 1000ppm Na Flame Photometer Standard 1000ppm K Flame Photometer Standard 1000ppm Li Flame Photometer Standard 1000ppm Ca Cleaning Solution 500ml Diluent Concentrate Tubing Disinfectant Deproteinising solution 80ml pack of 6 24 March 2012 ECN 736 per metre per metre 1 pack o nl n i nl oc i QUANTITY 6 x 100ml 6 x 100ml 6 x 100ml 6 x 100ml 1 bottle 6 x 100ml 6 x100ml 1 pack 46 Model 410 amp 410C Flame Photometer with Interface Appendix A Bibliography Reference l Re ae a SE TEA 410 91 001 Kolthoff I M Z Anal Chem 70 397 1927 Shohl A T and Bennett H B J Biol Chem 78 643 1928 Hald P M J Biol Chem 167 499 1947 Bernstein R E S Afr J Med Sci 17 101 1952 Alkemade C T J Smit J and Verschure J C M Biochimica et Biophysica Acta 8 562 1952 Collins G C and Polkinthorne H The Analyst 77 917 pp 430 436 1952 Dryer R L Clin Chem 2 112 1956 Puffeles M and Nessim N E The Analyst 82 976 1957 Boling E A J Lab and Clin Med 63 501 1964 Amdisen A Scand J Clin and Lab Invest 20 2 104 1967 Issue 7 24 March 2012 ECN 736 47 Model 410
48. race Continue with Section 4 Operating Instructions Issue 7 24 March 2012 ECN 736 14 3 Model 410 amp 410C Flame Photometer with Interface Performance Characteristics and Specification 3 1 3 2 3 3 3 4 Readout 342 digit light emitting diode LED display 12 5mm high Display range 0 to 1999 199 9 with decimal point on NOTE If negative values are displayed the instrument is operating outside of the recommended measurement range Measurement Ranges Na 0 10ppm linear Above this range Na is non linear due to self absorption A lineariser can be fitted which will linearise concentrations up to 20ppm Na Part number 41086001 Fitted as standard to the 410C K Li amp Ca Oto 100ppm Ba 0 3000ppm These are sample concentrations directly entering the flame photometer after dilution Specificity For Na K and Li measurements interference will be less than 0 5 from other elements at the same concentration equal to the concentration of the element under test Ca and Ba will normally be less than 5 interference from other elements at the same concentration Accuracy Linearity Better than 2 measured at mid range when standardized at the following concentrations 3ppm Na 3ppm K amp Sppm Li e g 3ppm Na calibrated to 100 on the display will read 49 to 51 at mid point Drift At zero Better than 2 per hour when calibrated at 1Oppm for Na K and Li and at 50 ppm for Ca and Ba At 10ppm Be
49. rately from the instrument Fit the mounting bracket to the rear panel with the two screws supplied and slot in the Natural gas regulator Figure 2 3 Make sure that the gas flow arrow on the regulator points to the right Connect the Natural gas supply to the Natural gas regulator inlet with the fuel tubing and Unex clamp supplied with the instrument If it is necessary to fit the filter because of suspected dirty gas then this should be fitted before the Natural Gas regulator by cutting the rubber hose and fitting the filter in the tubing with the arrow pointing towards the regulator Secure the tubing to the filter and regulator with the extra Unex clips supplied with the Natural Gas regulator kit Connect the Natural gas outlet tubing supplied with the regulator to the gas inlet connector item 4 Figure 2 2 using the connector and nipple removed as described in paragraph 2 above bypassing the Propane Butane regulator Secure with Unex clamp supplied Figure 2 3 Natural Gas Regulator 1 Regulator screw cap 2 Filter in line with Rubber hose supplied with instrument 3 Natural gas outlet tubing 4 Mounting bracket Remove the screw cap item 1 Figure 2 3 Rotate the screw head fully clockwise and then counterclockwise exactly one turn Refit screw cap Return to point 6 of Section 2 4 on page 12 410 91 001 Issue 7 24 March 2012 ECN 736 55 Model 410 amp 410C Flame Photometer with Interface S
50. rating temperature to stabilize This will ensure a stable burner temperature when solutions are aspirated after the warm up period During the warm up period prepare a set of calibration solutions to cover the required measurement range To obtain maximum linearity Sherwood Scientific recommends that the highest standard concentration does not exceed 10ppm for Na and K and 100ppm for Ca While aspirating diluent adjust the blank control so that the display reads 0 0 Aspirate the highest concentration standard Allow 20 seconds for a stable reading and then adjust coarse and fine controls for a convenient reading e g 20ppm Na can be set to read 20 0 on the display Carefully adjust the fue control for a maximum reading on the display ensuring that only small adjustments are made with a pause of several seconds between adjustments NOTE If you have any difficulty obtaining a maximum sodium reading proceed as follows Open the inspection flap and adjust the fuel control until the flame just starts to lift off the burner Then turn the fuel control back counterclockwise until the cones of the flame are on the burner Close the flap and proceed with paragraph 11 Remove the standard solution wait 10 seconds then aspirate a blank solution of diluent for 20 seconds Adjust the blank control for a 0 0 reading Remove the blank solution and wait 10 seconds Repeat paragraphs 8 9 and 11 until the blank reading is 0 0 within 0 2 and the
51. rint button to print 16 Aug 06 09 02 36 Sn2 Calib xx Aspirate the sample press the selector button once to illuminate S When the display is stable press the print button the printer will print out 16 Aug 06 09 05 24 Sn3 Sample xx Aspirate the next sample and press print when the display has stabilised The print out will show the result and incremented sequence number 16 Aug 06 09 06 01 Sn4 Sample xx If a sample is to be repeated you may repeat the sequence number by pressing the select button to illuminate R Aspirate the sample again and press print when the display is stable S will illuminate the print out will show 16 Aug 06 09 06 59 Sn4 Repeat xx To re set the sequence number press and hold down both the Select and Print buttons for more than 0 5s The buzzer will sound No change will be observed in the LED s and the printer will not print When the Print button is next pressed the Sequence number printed will be 1 410 91 001 Issue 7 24 March 2012 ECN 736 28 Model 410 amp 410C Flame Photometer with Interface Operating Instructions continued 4 4 8 Interface Operation continued With A Computer Connect the computer serial port to the instrument with the cable provided and switch on the computer Open HyperTerminal on the computer see Appendix C and use the following for the communication settings 9600 baud 8 bit No parity Stop bit 1 Flow Control None Switch on
52. se will determine how frequently the calibration needs to be checked Issue 7 24 March 2012 ECN 736 26 Model 410 amp 410C Flame Photometer with Interface Operating Instructions continued 4 5 Shutdown Procedure Aspirate Cleaning Solution diluted 1 in 100 with deionised water for one minute Aspirate diluent for two minutes For a short term shutdown two hours switch off the mstrument power switch and switch off the compressor For a longer term shutdown overnight turn off the fuel supply at source When the flame on LED is extinguished switch off the power switch and the compressor This ensures that the fuel pressure in the fuel tubing is at a minimum 4 6 Operating Hints l 410 91 001 The deionised or high quality distilled water used when making a batch of diluent must be free from contaminating elements It is recommended that the same batch of diluent is used to prepare all solutions and to set blank on the instrument Greatest accuracy will be obtained by using the same dilution equipment for both standard and sample preparation Always use suitable standards for calibrating the instrument Remember that the accuracy of the results obtained from the Model 410 depend on the accuracy and purity of the calibration standard that is used The nebuliser is a precision assembly contributing more than any other single item to the effectiveness of your Flame Photometer To keep it in good condition it 1s essen
53. terface Appendix C HyperTerminal This program is available in all versions of windows software To access HyperTerminal and set it up to communicate with your new module Select All Programs Accessories Communications HyperTerminal New Connection Name e g M410 Flame Comsl Port Settings Bits per Second 9600 Data Bits 8 Parity None Stop Bits 1 Flow Control None Apply 410 91 001 Issue 7 24 March 2012 ECN 736 53 Model 410 amp 410C Flame Photometer with Interface Appendix D Installing a Natural Gas Regulator Kit l Remove the fuel restrictor item 5 Figure 2 1 and connect the fuel tubing item 6 Figure 2 1 directly to the mixing chamber connection 2 Unscrew and remove the gas inlet regulator inlet and regulator outlet connectors items 4 5 and 6 Figure 2 2 and remove the copper tubing item 2 Figure 2 2 3 Connect the regulator inlet with connector item 5 Figure 2 2 to the gas inlet item 4 Figure 2 2 Figure 2 2 Rear Panel 1 Natural gas regulator assembly see Figure 2 3 2 Copper tubing 3 Air regulator adjuster 4 Gas inlet connector 5 Regulator inlet connector 6 Regulator outlet connector 410 91 001 Issue 7 24 March 2012 ECN 736 54 Model 410 amp 410C Flame Photometer with Interface Appendix D continued Installing a Natural Gas Regulator Kit continued 9 Locate the Natural gas regulator and mounting bracket ordered as optional accessories and packed sepa
54. than operator maintenance as described in the instructions repairs or modifications have been made by Sherwood or Sherwood authorised personnel This product has where applicable been operated using Sherwood specified supplies and reagents Sherwood reserves the right to make any changes in the design or construction of future products of this type at any time without incurring any obligation to make any changes whatsoever to this particular product Reagents supplies consumables accessories and user maintenance items are not included in this warranty Repairs or replacement of any part failing due to abnormal conditions including the following are excluded from this warranty a Flood lightning earthquake tornado hurricane or any other natural or man made disaster Fire bombing armed conflict malicious mischief or sprinkler damage Physical abuse misuse sabotage or electrical surge d Damage incurred in moving the product to another location OE 410 91 001 Issue 7 24 March 2012 ECN 736 56 Model 410 amp 410C Flame Photometer with Interface Product Warranty Statement continued 8 User agrees to permit Sherwood personnel or personnel of its authorised distributor to make changes in the product which do not affect results obtained but do improve product reliability Representations and warranties purporting to be on behalf of Sherwood made by any person including distributors and representatives of Sherwood
55. the system use Tubing Disinfectant EQUIPMENT REQUIRED Deproteinising Solution or Tubing Disinfectant l Light the flame as detailed in Section 4 4 Zi Present a beaker of Deproteinising Solution or Tubing Disinfectant to the nebuliser for 10 minutes 3 After 10 minutes have elapsed replace the beaker of solution with a beaker of deionised water Flush the system for two minutes 4 Shutdown the instrument as detailed in Section 4 5 410 91 001 Issue 7 24 March 2012 ECN 736 38 7 1 7 2 Model 410 amp 410C Flame Photometer with Interface Troubleshooting Power on LED not illuminated Check that the instrument is connected to a working a c supply and that the power switch is depressed Check the plug fuse and replace if necessary Check the instrument fuses and replace if required refer to Section 6 9 If fault persists contact your Sherwood Scientific Distributor Flame on LED not illuminated General Ensure that the U tube is filled with deionised water refer to Figure 2 4 Burner must be fitted with pointed end downwards refer to Section 6 7 Air Supply Air compressor must be connected to an a c supply and working correctly Check the air tubing connections from the air compressor to the instrument refer to the air compressor manual Fuel Supply There must be a compressed air flow otherwise the air pressure switch will not allow the gas to flow into the instrument Check that there
56. tial that it is flushed with distilled water after use This ensures that no solid matter remains in the assembly If this procedure is carried out regularly no difficulties should be experienced Applications and Method Sheets are available from your Sherwood Scientific distributor or www sherwood scientific com A guide to Flame Photometry is available from your Sherwood Scientific Distributor Issue 7 24 March 2012 ECN 736 DI Model 410 amp 410C Flame Photometer with Interface Operating Instructions continued 4 7 Interface Operation With A Printer Connect the printer to the instrument with the cable provided and switch on the printer Make sure that the printer is set to 9600 baud 8 bit No parity Stop bit 1 Switch on the flame photometer When the start up sequence has completed the module will be energized this is indicated by the illumination of the printer symbol and B for blanking The following will be printed Flame Photometer Data Output Rev x xx Serial Number xxxxx Aspirate the blank solution and adjust the zero control to get 000 on the display When the display is stable press the print button under the printer symbol the printer will print out 16 Aug 06 09 01 45 Sn1 Blank xx Next aspirate the top calibration standard and press the selector button once to illuminate C Adjust reading to the required setting using the fine and coarse controls When the display is stable press the p
57. tlet connector Secure with Unex tubing clamps supplied Ensure that the connector at the regulator inlet is tightened to 12nm torque NB The connection at the cylinder end of the tube must comply with National regulations Turn on the fuel supply and check all connectors for leaks using soap solution Do not use the instrument until you are satisfied that the installation is leakproof Connect the length of reinforced Nylon hose supplied between the air compressor outlet and the air inlet connector on the rear panel Secure both ends with Unex tubing clamps supplied Fit a length of drain tubing 400 22 000 item 4 Figure 2 4 to the outlet on the constant head drain or Gas Trap If necessary connect a suitable length of tubing not supplied to extend the drain tubing to carry waste to a sink or other drain receptacle The downward flow of waste must not be restricted oa ee Figure 2 4 Filling the U Tube 1 Wash bottle 2 Constant head drain Gas Trap 3 U tube 4 Drain tube 5 Spring clip Issue 7 24 March 2012 ECN 736 ee Model 410 amp 410C Flame Photometer with Interface Installation continued 2 4 Assembly continued 9 10 11 410 91 001 Make sure that the constant head drain Gas Trap is positioned so that it is pushed fully down on its retaining screws Use a wash bottle item 1 Figure 2 4 to fill the U tube with deionised water Sufficient water should be us
58. to 132V 220V 198V to 229V 240V 230V to 264V Issue 7 24 March 2012 ECN 736 D Model 410 amp 410C Flame Photometer with Interface Installation continued 2 3 Unpacking l Unpack the instrument and accessories NOTE The Model 410 weighs 9 5kg follow safe lifting techniques 2 Check all items for damage 3 Check that all the items on the Accessory List have been delivered Contact your Sherwood Scientific distributor if you have any problems 4 The Model 410 is shipped with the following items Accessory List CAT NO ITEM QUANTITY 001 72 043 Air tubing nylon reinforced 2 metres 001 72 114 Fuel tubing 2 metres 400 22 000 Drain tube all flame 20411 on 1 metre 001 48 105 Fuse 200 mA slo blo spares 2 471 71 900 Supply lead a c 1 100 99 010 Nebuliser cleaning wire pack of 3 1 pack 001 31 076 O ring spare for mixing chamber 1 420 08 102 Nebuliser 1 400 22 003 Nebuliser inlet tube polythene 150mm 1 410 91 001 Operators Manual English 1 001 08 718 Unex tubing clamp 4 410 92 001 CDROM Flame Training Guide 1 410 09 003 Lead M410 to Printer Computer 1 supplied with the M410 Industrial only see addendum B section 8 4 for M410 Clinical Standards 001 56 620 Flame Photometer Standard 1000ppm Na 100m 1 pack of 6 001 56 621 Flame Photometer Standard 1000ppm K 100ml 1 pack of 6 001 56 623 Flame Photometer Standard 1000ppm Ca 100ml 1 pack of 6 410 91 001 Issue 7 24 March 2012 ECN 736 10
59. tter than 2 per 5 minutes for Na K Li Ca and Ba based on 10 consecutive samples aspirating sample for 20 seconds then 10 seconds of air NOTE A minimum 30 minutes warm up must be allowed to meet the drift specification refer to Section 3 5 Reproducibility NOTE The following specification is correct for use on Propane or Butane If Natural Gas is used the reproducibility may be slightly worse Less than 2 CV for 20 consecutive readings of the same bulk sample aspirating sample for 20 seconds then 10 seconds of air The concentration of the bulk sample to be 10ppm for Na K and Li which will give a standardized reading of 100 on the display i e 10 0 with decimal point on 410 91 001 Issue 7 24 March 2012 ECN 736 15 Model 410 amp 410C Flame Photometer with Interface Performance Characteristics and Specification continued 3 5 3 6 3 7 3 8 3 9 Warm Up To achieve the stated specification the flame must be alight for a minimum of 30 minutes with diluent being aspirated Sample Requirement Type Dependent on application However samples should be water based and not highly viscous nor non homogeneous Organic solvents affect the air fuel ratio could attack the mixing chamber materials and can affect the safety operation of the Gas Trap constant head drain and should be used with great caution Method of presentation The sample is presented to the nebuliser from a sample cup test tube or other su
60. y be too high Make sure the correct filter has been selected If there is a chance that the optical filters may have been serviced check that they have been fitted correctly refer to Section 6 8 The nebuliser may be blocked Check the nebuliser refer to Section 6 6 Check the glass chimney is clean refer to Section 6 8 Make sure the fuel control is set correctly refer to Section 4 3 If the atmosphere surrounding the instrument or air compressor inlet 1s contaminated e g smoke incorrect readings may result If fault persists contact your Sherwood Scientific Distributor Unstable results Do not use compressed air from a communal source or Air Line as this may be contaminated with oil which will ruin the performance of the Model 410 Use a dedicated Compressor such as the Sherwood Model 851 or Model 855 Check the concentration of the solutions being aspirated The solutions should all contain 1 in 1000 dilution of Diluent Concentrate refer to Section 4 4 Check the fuel supply is adequate Make sure the fuel control is set correctly refer to Section 4 3 The use of Natural Gas from a communal source will fluctuate as other users turn on or turn off their gas taps Wherever possible use bottled gas The nebuliser may be blocked Check the nebuliser refer Section 6 6 The U tube or drain tubing may be blocked or partially obstructed If the air supply tubing show signs of condensation drain the a
61. y if e Assembly operations extensions re adjustments modifications or repairs are only carried out by persons authorized by them e The electrical installation of the relevant room complies with IEC requirements or the local regulatory code e The equipment is used in accordance with the instructions for use The information contained in this manual was correct at the time of going to print However Sherwood Scientific s policy is one of continuous product improvement and the right to change specifications equipment and maintenance procedures at any time without notice is reserved This manual is copyrighted and all rights are reserved No part of this manual may be reproduced by any means or in any form without prior consent in writing Sherwood Scientific Ltd 1 The Paddocks Cherry Hinton Road Cambridge CB1 8DH United Kingdom Tel 44 1223 243444 Fax 44 1223 243300 Email info sherwood scientific com www sherwood scientific com 410 91 001 Issue 7 24 March 2012 ECN 736 Dia Contents Model 410 amp 410C Flame Photometer with Interface Page 0 IRR ee 2 RAR O OO O OOO 6 1 1 COLI 8 LU 180 e a E E 6 1 2 ad A A ere ere ere re 6 1 3 RL RO 7 1 4 tei PEENE AIA 7 BIO O E ON e 7 UL AR o I ET 7 REI RR EE 7 AGO EE E t_c 3 Lal edile 3 ai ee ae eee eee eee eee ee rer ne ae ee er see rer or ere re re a o o wey enw oseess ony vay en dicoeensy env vay ee dicoee ney unvuny ee saeeens
62. y une vaweessensway ane varessverewavensveues 8 ee A A A A A A E 8 bE AB E E EEEE EE EEEE EE EEEE OOTE 8 pie vaio Sa eee 9 2 3 E E A E li 10 Accessory List sseessseesseessserssseesserssserssersssrrssersssrrsseeossresseesssersserosseessseosseesseeo 10 24 A T a a Tn 11 2 eu ee eee eer ee eee er ere er errr errr errr 14 2 6 CATE LI ENRE NE NEN RE USEE EEE UAEDEE 14 3 Performance Characteristics and SOCIA 15 3 1 CCI RR RR E EA 15 di VE cdi e A 15 3 3 EAL RR 15 3 4 ACCULACY ie 15 EC ARRE 15 ti roi 15 TEU ALLEA RR RR 15 ay Warm Up ii 16 3 6 VEGA A EEA 16 ti e eer ere eer er ere eer er ere eer erry rere rer re rere 16 ke med i a 16 COR TITO O NOOO 16 LT RR RE e e ieia 16 3 8 EI n 16 3 9 Dr Os vow sss RE CORGCA GE CRE GA Ene GOLA EENEN ENKEN QRS NEERU NEDENE UENEN EEKE RO TT TTT TTT 16 ici FL 16 ih A er ere ere 16 Ri A ere eer ere ere rere re eer errr ee 16 CA UE EOLICI ee nen nt RI 17 Voltage 17 EEEE EROE TIRA 17 AEn aa E EE E E E E E E ee EN 17 ic rocco nto 17 s dI _ ete ees tee mens sae eens es eevee ee ee wes ne 17 e Ie 17 i III 17 410 91 001 Issue 7 24 March 2012 ECN 736 de Model 410 amp 410C Flame Photometer with Interface Contents Page Be C E ses pd Sa sy Stepney zag I alal 18 4 1 Front Panel Controls and Indicators See Fig 5 1 18 di 18 Ana 18 i ATTO 18 Sensitivity Fine and Coarse 18 illa 1

41091001 M410 & 410C with Interface Operator Manual Issue 7

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41091001 M410 &amp; 410C with Interface Operator Manual Issue 7

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41091001 M410 & 410C with Interface Operator Manual Issue 7