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Fisher Dry Bath Incubators Instructions

1. 105039087 2 Sensor PC Board 105028030 2 Temperature Sensor 105028031 2 Blower Whee 1402 020 2 She SS 102660011 24 102013005 1 CO Filter 9101285 1 Power Board Assembly 00374701 2 Control Board Assembly 20222 00374901 2 Glass DOOR 330260 2 Glass Door Assembly es 107002003 2 Float Switch Assembly 105013039 2 Power COP 353025 1 SS 102644010 10 Water Full OSG 2 2 0 108001004 1 HEPA Gas Fiol 542575 2 1 Phone Plug 1 4 CMS 1418 040 2 Relative Humidity Sensor DATOS 2 Humidity 102633001
2. 330260 2 Glass Door Assembly 107002003 2 Float Switch 105013039 2 Power 353025 1 A 102644010 10 Water Fill HOSE 108001004 1 Gas Filer tes 1 Phone Plug 1 4 CMS es 1418 040 2 Relative Humidity Sensor 541951 2 Tray 102633001 2 35 AUTION STATIC SENSITIVE DEVICE PART 4 ILLUSTRATIONS FIGURES AND SCHEMATICS Pister Scieatific co2 SENSOR SEC TEMP DISPLAY CALIBRATION 8 DISPLAY CALIBRATION 9 SAFETY SET POINT CALIBRATION 10 DISPLAY CALIBRATION 11 PRESS IN TO RELEASE CONTROLLER ASSEMBLY NOT USED ON THESE MODELS 11 687 5 11 687 10 11 887 51 11 887 101 Figure 2 Controller Board Removal 36 SOLENOID VALVE CONNECTOR 6 TRANSFORMER INPUT VOLTAGE SELECTOR TEMPERATURE TRANSFORMER CONNECTOR J 1 FLOAT SWITCH CONNECTOR 2 7 JUMPER 6 1 VOLT VOLTAGE REGULATOR U 2 1 V
3. 143304100 4 Chamber Heater Outer Top 247423 2 223720 2 297047 2 Limiting Thermostat es 239190 2 Power Transformer 6 105039087 2 Sensor PC Board 105028030 2 Temperature Sensor 2 2420242422 105028031 2 Blower Wheels bigs 1402 020 2 Shelf 102660011 24 CO Filter Extena es an 1020130065 1 31012840550 1 Power Board Assembly 00 04 0 00374701 2 Control Board Assembly pp 00374902 2 EJE A ETA 330260 2 Glass Door Assembly 04 107002003 2 Float Switch 105013039 2 Power Cord 353026 1 Shelf 1026
4. 00374901 1 Glass DOOF 330260 1 Glass Door Assembly 1070020038 1 Float Switch 105013039 1 Power Cords 353025 1 Shelf 102644010 5 Water Fill cece cceessereeresstactescerseecesttensersessneeterers 108001004 1 HEPA Gas Filter Kit 5425795 1 Phone Plug 1 4 8 1418 040 1 Relative Humidity Sensor Assembly 541951 1 Humidity 102633001 1 31 REPLACEMENT PARTS LISTS MODEL 10 110 120 VOLTS 50 60 Hz DESCRIPTION PART NO STD QTY Foot Levees 241077 4 Power Board 3 8 101408009 2 Fuse 5 5 X 20MM 28911422220 09 2 Blower Duct Air 102625010 2 101432011 1 D aa At m LT E AREE E E 247426 2 sc 247431 2 Cartridge
5. Filter External CO Filter Int var Power Board Assembly Control Panel Glass Door 0446 tere ees Glass Door Assembly Float Switch Power Cord Phone Plug 1 Relative Humi Humidity Tray 4464444 0 4 0 6 CMS dity Sensor Assembly 29 PART NO STD QTY 2410 4 101408009 1 2 102625010 1 101432011 1 24426 1 247431 1 143304100 2 247423 1 20220 1 297047 1 239190 1 105039087 1 105028030 1 105028031 1 1402 020 1 102660011 12 102013005 1 S 1012 Baiiia naas 1 00374701 1 00374901 1 230260 1 107002003 1 105013039 1 992025 1 102644010 5 108001004
6. 2 33 REPLACEMENT PARTS LISTS MODEL 101 110 120 VOLTS 50 60 Hz DESCRIPTION PART NO STD QTY 4 Power Board Fuse 3 8 101408009 2 Fuse 5 AMP 5 X 20 0 40 4 7000 0 0 289114 2 Blower Duct Air 102625010 2 101432011 1 247426 2 247431 2 Cartridge 143304100 4 Chamber Heater Outer 2474231 2 223120 2 Solenoid 297047 2 Limiting Thermostat ss 239190 2 Power 105039087 2 Sensor PC Board Assembly 102204101 2 Temperature Sensor 105028031 2 ie 1402 020
7. 2 Shelf Slides eeen tni 102660011 24 Filter sotnie assenteisti serisi 102013005 1 Filter Internal 310128 1 Power Board 00374701 2 Control Board Assembly 2 42 00374902 2 330260 2 Glass Door 107002003 2 Float Switch Assembly te 105013039 2 Power ieee 35302 0 1 102644010 10 Water Fill 108001004 1 HEPA Gas 54257625 1 Phone Plug 1 4 CMS 1418 040 2 Humidity Tray cance 102633001 2 34 REPLACEMENT PARTS LISTS MODEL 101H 110 120 VOLTS 50 60 Hz DESCRIPTION PART NO STD QTY Foot LEVGIGNS 24107 4 Power Board Fuse 3 8 101408009 2 Fuse 5 5 X 20MM 4 2 Blower Duct Air 102625010
8. 12 120 volt heaters 140 ohms 10 cold resistance 4 With Connector J 3 still removed from the power circuit board measure resistance from heating element case to one of its wires Select highest range on ohmmeter Do not touch test leads with hand Meter should read infinity Any reading less than infinity indicates cartridge heater is shorting to ground Replace faulty cartridge heater Refer to Section 3 8 for cartridge heater replacement To test the air heater s 5 Disconnect the wires at air heater terminals See Figure 6 Measure the resistance across air heater terminals Readings should be the same as for the cartridge heaters shown above 6 Next measure between heater sheath and heater terminals Meter should read infinity 7 heater does not meet the above specifications replace air heater Refer to Section 3 9 for air neater replacement 2 6 DOOR HEATER There is a door heater inside each door attached to the door liner See Figure 4 Each heater has two sections Each section is rated at approximately 40 watts This yields approximately 80 watts per door The door heater is controlled by the chamber heater circuit When the chamber heaters are on the door heater is on The door heater eliminates a majority of the moisture on the glass door The heaters are connected in a parallel connection for 120 volt operation To test the door heater 1 Remove Connector J 3 from the powe
9. contact with heater 3 Properly position base 2 Make sure that wires are routed so as not to come in checking alignment of thermostats as in Thermostat Replacement section Replacement Parts Item Thermostat Low Temp Thermostat High Temp Selector Switch Indicator Lamp Dry Block Handle Heater Base Assembly for 11 718 Heater Base Assembly for 11 718 2 Heater Base Assembly for 11 718 4 Heater Base Assembly for 11 718 6 Heater Base Assembly for 11 718 8 _ 4 Fasten base to top cover and replace knobs Part Number 83310 83311 83364 64057 83326 83305 83306 83307 83308 83309 61 REAR VIEW 3 1 SPLICE 2 ACCESSORIES Item Capacity Cat no Dry Block 24 tubes 1 5ml 11 718 9 Dry Block 30 tubes 6mm diameter 11 718 10 Dry Block 20 tubes 10mm diameter 11 718 12 Dry Block 20 tubes 18mm diameter 11 718 14 Dry Block 12 tubes 16mm diameter 11 718 16 Dry Block 8 tubes 20mm diameter 11 718 18 Dry Block 6 tubes 25mm diameter 11 718 20 Dry Block Combination 11 718 22 6 tubes 6mm diameter 5 tubes 13mm diameter 3 tubes 25mm diameter Part No 83315 Instructions for Dry Bath Incubators Rev A 12 91 Fisher Scientific 711 Forbes Ave Pittsburgh Pa 15219 1 800 388 8355 REAR VIEW SPLICE 3 SCHEMATIC FORREPAIR INFORMATION OR REPLACEMENT PARTS ASSISTANCE PLEASE CONTACT YOUR NEAREST CUSTOMER SUPPORT CENTER LISTED IN THE BACK OF THIS MANUAL SERVICE M
10. 2 101432011 1 247426 2 247431 2 HOGER 143304100 4 Chamber Heater Outer pp 247423 2 Fan 223720 000 4 2 Solenoid eE S 297047 2 239190 2 Power Transformer Ne 105039087 2 Sensor PC Board Assembly 102204101 2 Temperature Sensor 55 105028031 2 Blower Wheel 1402 020 2 Shelf 102660011 24 Filter 102013005 1 4 90 5 310128 1 Power Board Assembly 2 2 2 00374701 2 Control Board Assembly 00374901 2 Glass DOO
11. 28 Model 100 120 Volts 50 60 HZ Ne 29 Model 51 100 120 Volts 50 60 2 24040 0002 30 Model 51H 100 120 Volts 50 60 2 31 10 100 120 Volts 50 60 2 4 042222 2 2 2 32 Model 10H 100 120 Volts 50 60 HZ 33 101 100 120 Volts 50 60 34 Model 101H 100 120 Volts 50 60 HZ2 35 PART 4 ILLUSTRATIONS FIGURES AND SCHEMATICS FIG NO PAGE 1 CONTROL PANEL 36 2 CONTROLLER BOARD REMOVAL 36 3 POWER CIRCUIT BOARD 37 4 DOOR HEATER amp ATTACHING 37 5 TOP COVER REMOVED 38 6 REAR VIEW COVER REMOVED 4 38 7 FAN MOTOR PLATE 39 8 VR INTERFACE BOARD AND REATED COMPONENTS 39 9 SHELF SLIDE 40 10 BLOWER DUCT REMOVAL Ne 40 Tit SHELF SUDE ASi 41 42 TOP COVER REMOVAL RN 41 13 DUAL CHAMBER UNITS FILL SIPHON PORT COVER REMOVAL 42 44 LOWER DOOR SUPPORT 42 15 UPPER DOOR SUPPORT BRACKET RN 43 WIRING DIAGRAMS amp SCHEMATICS pe 44 U S CUSTOMER SUPPORT CENTERS R
12. Isolate the wire nut connections within the insulation Re position wires and connectors and then replace all covers 3 11 AUXILIARY CHAMBER HEATER Disconnect electrical service Remove the screws from the top cover Remove 2 screws from each end of the fill siphon port cover See Figure 12 Slide the top cover forward about 1 2 inch to release it from the front of the enclosure Lift top up and disconnect the sample port tubing Remove top from the unit Forthe lower chamber remove 2 screws from each end of the lower fill siphon port cover See Figure 13 Grasp each end of the cover and push down to release the front edge from the upper splash cover Lift cover out and remove sample port tubing Remove the wire nuts from the heater wires Carefully remove heater from top of chamber Note the location for replacement purposes location of holes in gasket See Figure 4 See Figure 5 19 Remove paper backing from new heater and apply adhesive side to chamber in same position with wires extending in same direction as faulty heater Be sure all air pockets are removed when applying new heater to top of chamber This will over vent hot spots Connect the wires as before Replace all covers 3 12 FLOAT SWITCHES Disconnect electrical service For the upper chamber remove the screws from the top cover Remove 2 screws from each end of the fill sipnon port cover See Figure 12 Slide the top
13. Uniformity FISHER DRY BATH INCUBATORS 11 718 11 718 2 11 718 4 11 718 6 11 718 8 Catalog Nos Fisher Dry Bath Incubators provide controlled heat for a wide variety of clinical and general chemistry applications They operate through a temperature range from slightly over ambient to 1390 The various models can accommodate 1 2 3 4 or 6 dry blocks Each unit is controlled by two adjustable thermostats one for operation up to about 60 C and the other for up to 130 A fully insulated pad type heating element is bonded directly to the entire underside of the dry block support plate to provide even heat distribution The support plate is made from aluminum for rapid heat transfer Each model is designed to be used with any of eight different accessory dry blocks Section for a listing of catalog numbers made from solid aluminum with a black anodized finish Each block is marked so that every well can be identified Dry blocks can handle a variety of tubes from 1 5 ml microcentrifuge tubes to 25 mm diameter tubes All of these accessory dry blocks are interchangeable See the Accessories Typical applications these dry baths include _incubation culture inactivations enzyme reactions melting and boiling points and many other procedures that require controlled heat Performance Characteristics Slightly Above Ambient to 130 Note Tolerances temperature ranges are
14. 5 Remove 3 or 4 screws securing the interface board to the back of unit See Figure 8 6 Remove the sensor cable from the CO sensor Remove the metal shield from the CO sensor and place on the new sensor This shield must be in place for proper shielding from electrical or magnetic interference 7 Replace the CO sensor assembly in the reverse of the above procedure NOTE IT IS IMPORTANT TO MECHANICALLY ISOLATE THE LOWER RIGHT MOUNTLNG SCREW OF THE INTERFACE BOARD FROM CHASSIS GROUND 3 5 TEMPERATURE SENSOR 1 Disconnect electrical service 2 Referto Section 3 1 on shelf support removal 3 Remove 4 nuts from the fan motor sensor plate and pull the plate in the chamber See Figure 7 4 Disconnect temperature sensor wires at Connector J 10 and remove sensor 5 Replace temperature sensor in reverse of the above procedure 3 6 RELATIVE SENSOR 1 Disconnect electrical service 2 Referto Section 3 1 shelf support removal 3 Remove 4 nuts from the fan motor sensor plate and pull the plate in the chamber See Figure 7 Disconnect temperature sensor wires at Connector J 10 and remove sensor Replace temperature sensor reverse of the above procedure 3 7 FAN MOTOR Disconnect electrical service Refer to Section 3 1 on shelf supports amp blower duct removal and remove these items Remove 4 nuts from the fan motor sensor plate and pull the plate into the chamber See
15. 9 DZ TRANSRORIMER 10 23 POWER PRINTED CIRCUIT 10 2 3 1 POWER BOARD 11 2 4 CONTROLLER 12 25 CHAMBER HEATER 12 28 o 13 27 AUXILIARY CHAMBER 13 ed 13 2 9 SOLENOID VALVE 14 216 SWITCH 14 2 11 TEMPERATURE SENSOR 15 2 42 HIE SENSOR 15 974 3 SENSOR 15 2 14 RELATIVE HUMIDITY SENSOR 15 PART 3 REMOVAL REPLACEMENT 17 3 1 SHELF SUPPORTS amp BLOWER DUCT 17 32 SOLENOID VALWES 17 92 TOCO SENSOR 17 34 SENSOR ASSEMBLY 17 35 TEMPERATURE SENSOR ee 18 36 RELATIVE HUMIDITY SENSOR 18 27 TRAN MOTOR 18 3 8 CARTRIDGE HEATERS 18 204 HEATER 19
16. Disconnect the wires from the solenoid valve Remove the tubing from the solenoid valve by turning the fittings 1 4 turn counterclockwise Remove screw s from the solenoid valve bracket Refer to Figure 3 4 Remove the fittings from the old solenoid valve Use an ample amount of thread sealant on the threads before installing the fittings into the new solenoid valve Do not allow the thread sealant to enter the solenoid valve It might cause the valve to malfunction The fittings are also fragile Do not apply excessive torque Replace the solenoid valve in the reverse of the above procedure 3 3 TIC CO SENSOR Disconnect electrical service Refer to Section 3 shelf support removal Remove 4 nuts from fan motor sensor plate and pull the plate into chamber Refer to Figure 7 Disconnect the sensor from Connector J 11 at the power circuit board Remove 4 screws securing the CO sensor assembly to fan motor sensor plate Replace the CO sensor in the reverse of the above procedure 3 4 CO SENSOR ASSEMBLY Disconnect electrical service Remove the rear cover Disconnect the sensor from the interface board Disconnect the 4 pin connector from the interface board The CO sensor will normally be replaced as an assembly which includes the interface board See Figures 3 amp 8 4 Remove 4 screws securing the CO sensor assembly to fan motor sensor plate
17. button adjust R 10 until the display reads between 0 5 and 1 0 C above the desired operating temperature Push the retainer in to release the control module Slide the control module back into the door before attempting to close the door 1 3 3 RELATIVE HUMIDITY CALIBRATION Normally the Relative Humidity Display will not require calibration As a matter of fact sophisticated equipment is required to verify calibration Home ype humidity gauges are not considered accurate Their accuracy drops off dramatically in the upper humidity levels where the inclbator normally operates 1 Place an accurate humidity probel on the middie shelf of the incubator Close the door and allow the unit to stabilize for a minimum of one 1 hour 2 Compare the reading of your humidity instrument with the Relative Humidity Display If the display and the humidity instrument reaings disagree open the oute door and remove two 2 screws from the top of the control module See Figure 1 the contro module up about three 3 Inches until it locks in place See Figure 2 On the right side of the control module are four 4 potentiometers The bottom potentiometer R 11 is used to adjust the R H display Adjust R 11 until the display agrees with your hummidity instrument Push the retainer in to release the contro module Slide the control module back into the door and close the outer door 1 3 4 CO CALIBRATION Now that the temperature c
18. 1 542579 1 1418 040 1 541951 cin sis 1 102633001 1 REPLACEMENT PARTS LISTS MODEL 51 110 120 VOLTS 50 60 Hz DESCRIPTION PART NO STD QTY Foot 24 7 4 Power Board Fuse 3 8 AMp 101408009 1 Fuse 5 5 X 20MM Ne 289114 2 Blower Duct Air Plenum 2 2 000 102625010 1 EMI RFI Fiten 101432011 1 ah avon Mise ashe Haast 247426 1 Alt TO ALOR 247431 1 Cartridge Heater 143304100 2 Chamber Heater Outer 1 22372065000 1 1 Solenoid 0 040 0000 297047 1 Limiting Thermostat 2 2 222 22 1 4 0011 239190 1 Power Transformer 105039087 1 I R Sensor PC Board Assembly 102204101 1 Temperature Sensor 10502
19. 340 DOOR HEATERS 19 3 11 AUXILIARY CHAMBER 19 SERVICE MANUAL TABLE OF CONTENTS cont SECTION PAGE 3 12 20 212 INNER GLASS DOOR 20 20 3 14 1 SINGLE CHAMBER 15 20 3 14 2 DOUBLE CHAMBER UNITS LOWER 21 3 14 3 DOUBLE CHAMBER UNITS UPPER 22 3 15 WATER JACKET CHAMBER 020 22 2 23 3 15 1 SINGLE CHAMBER UNITS ccccssccsssesecscescesssscsressssessesecenseesecsases 23 3 15 2 DOUBLE CHAMBER UNITS LOWER 24 3 15 3 DOUBLE CHAMBER UNITS UPPER 25 3 10 FUSES even net cb cc am 26 3 17 CONTROLLER BOARD ASSEMBLY 22 04 00404400000 26 3 18 POWER CIRCUIT BOARD Nt 27 319 TRANSFORMER assed teva tana 27 3 20 222 27 3 21 REPLACEMENT PARTS LIST 28 Model 5 100 120 Volts
20. 5 Water 56 108001004 1 HEPA Gas Filter 5425675 1 Phone Plug 1 4 CMS 1418 040 1 Humidity 22222522 102633001 1 28 REPLACEMENT PARTS LIST MODEL 110 120 VOLTS 50 60 Hz DESCRIPTION Foot Levelers eee eee eee See eee er ee Tree rer ees Power Board Fuse 3 8 Fuse AMP 5 X 20MM 5 Blower Duct Air Plenum 2 2 2 2 2 EMI RFI Filter Door Heater Air Heater eee eee eee eee eee ee errr ere rere ce reer rrr rr errr eer reer sy Cartridge Chamber Hea Fan Motor ter Outer 040402222 2 Limiting Thermostat Power Transformer ee T C Sensor PC Board Assembly Temperature Sensor Blower Shelf Slides 060000 060 606 4988
21. 5 and 1 0 C above the desired operating temperature The desired operating temperature was determined when you selected the temperature with the set point switches This will allow the incubator to heat properly under normal conditions In the unlikely event of a primary control failure the back up control acts as a guard to prevent the incubator chamber from overheating more than this set value Proper calibration of the safety set point can not be overstressed From this you can also see that if the back up overtemperature control is set below the desired operating temperature the incubator will never heat So if you decide to change the desired operating temperature you must readjust the back up overtemperature control If the overtemperature control set pointis set equal to or less than the desired operating temperature the HI temperature LED wil flash and the audible alarm will sound 1 Press the SAFETY SET button If the displayed reading is between 0 5 and 1 0 C above the desired operating temperature then no further calibration of the back up overtemperature contro is needed 2 If calibration is required then open the outer door and pull the control module up about three 3 inches until it locks in place See Figure 2 On the right side of the control module are four 4 potentiometers The third potentiometer R 10 is used to adjust the back up control While pressing the SAFETY SET PT
22. Figure 7 Disconnect Connectors J 4 J 10 and J 11 from the Power board Remove the fan motor wires from Connector J 4 Terminals 7 amp 8 Remove blower wheel from fan motor shaft Remove 2 screws from fan motor and remove tan motor On models 51 51H 101 or 101H remove the metal shield This shield must be placed on the new motor for proper shielding of the CO sensor Replace fan motor in reverse of above procedure NOTE BE SURE MOTOR ROTATION IS CORRECT ROTATION SHOULD BE COUNTERCLOCKWISE WHEN VIEWED FROM REAR OF INCUBATOR 3 8 CARTRIDGE HEATERS Disconnect electrical service Remove rear cover Carefully remove heater wires from Connector J 3 at Power board Refer to Figure 6 Remove the nuts holding the heaters to the chamber 18 5 Slide heating element s out from chamber See Figure 6 6 Replace cartridge heaters in reverse order of above procedure CAUTION RECONNECT HEATING ELEMENT WIRES EXACTLY AS REMOVED TO ASSURE PROPER OPERATION IN CASE OF DIFFICULTY REFER TO THE ELECTRICAL WIRING DIAGRAM FOR THE PROPER HEATER WIRING ARRANGEMENT FOR YOUR ELECTRICAL SERVICE 3 9 AIR HEATER 1 Disconnect electrical service 2 Refer to Section 3 1 on shelf supports amp blower duct removal and remove these items 3 Remove 4 nuts from the fan motor sensor plate and pull the plate into the chamber See Figure 7 4 Disconnect Connectors J 4 J 10 and
23. I R CO sensor is not significantly affected by changes in operating relative humidity Relative humidity is measured by using a solid state type humidity sensor mounted on the motor plate The snsor monitors the relative humidity level within the chamber which in tum is diaplayed on the front panel It will monitor conditions from ambient to 99 0 relative humidity The tenths digit always fixed at Relative humidity is provided by pouring distilled water directly into a tray whicy can be placed on a helf or if desired by pouring the water directly onto the chamer floor Pouring th water onto the floor will hep maintain the highest relative humidity obtainable However this water should not be placed directly on the chamber floor when active chemicals to reduce fungus and mold growth are involved These units are also equipped with a temperature recorder jack and a provision for connection to a central monitoring system The temperature recorder jack allows the user to monitor each incubator operating temperature independently by connecting a voltage type chart recorder to this jack In this way complete temperature documentation can be provided along with the experiment or project The output to the recorder is 10 mv C This output does not monitor The 1 4 phone jack provided next to the recorder jack can be used to connect the incubator to a central monitoring system The jack can be wired either a
24. VOLTAGES BE CHECKED AND ADJUSTED AS REQUIRED REFER TO SECTION 2 3 REGARDING POWER BOARD CALIBRATION 3 19 TRANSFORMER 1 Disconnect electrical service 2 Remove rear cover 3 Disconnect Connector J 1 from the power circuit board See Figure 3 4 Remove the mounting screws from the transformer 5 Remove the connector from the old transformer carefully noting the location of each wire The connector will be used on the replacement transformer 6 Replace transformer in reverse order of above procedure 3 20 FILTER 1 Disconnect electrical service 2 Remove rear cover 3 Disconnect the wires from the EMI filter 4 Remove the mounting screws from the EMI filter and remove filter Remove the fuses from the old EMI filter They will be used again 5 Replace the EMI filter in reverse order of above procedure 27 3 21 REPLACEMENT PARTS LISTS MODEL 5 110 120 VOLTS 50 60 Hz DESCRIPTION PART NO STD QTY Foot 2 4 Power Board Fuse 3 8 101408009 1 Fuse 5 AMP 5 X 20MM 0 0 0240 02000000 289114 2 Blower Duct Air 1 102625010 1 EMI RFI FIET 101432011 1 Door 247426 1 Heate
25. also check for line voltage at connector J 4 Terminals 7 and 8 on the power circuit board If voltage is present disconnect motor and check continuity of coil If coil is open replace motor Refer to Section 3 7 for fan motor replacement 2 9 CO SOLENOID VALVE The CO solenoid vaive is of the normally closed configuration mounted at the lower left edge of the power circuit board Refer to Figure 6 in the Illustration Section of this manual It is important to maintain a minimum of 20 PSI 1 5 BAR pressure to maintain the proper flow of CO to the chamber 5 Volts DC applied to the coil activates opens the solenoid valve The signal is supplied through the power To test the CO valve 1 Set the CO set point to 20 0 The green LED for CO should be The valve should be energized at this time The valve makes an audible click although not very loud when energized Monitor the voltage at the solenoid coil 2 Sf the valve does not energize disconnect both wires at the coil and measure the resistance The resistance should be approximately 53 ohms 10 If not replace solenoid valve Refer to Section 3 2 for solenoid valve replacement 3 When the valve is closed there should not be any CO flowing The valve should be Bubble Tight Test this condition by placing the end of the CO injection tubing in a beaker of water There should be no bubbles Replace solenoid valv
26. chamber module by sliding chamber out of the front of the exte rior body These chambers are awkward to handle and heavy Another person to aid in removal is advised If returning chamber to factory for repairs or replacement remove the fan motor mounting bracket and float switch These items will be used again 11 Replace water jacket chamber module in reverse order of steps 6 through 10 above 12 There are adjustment points at the upper and lower door mounting brackets so that the outer door may be adjusted to close properly The outer door gasket should contact the incubator body evenly on all four sides If the door needs adjustment proceed as follows a With a wrench loosen the two nuts near the base of the lower door hinge See Figure 14 Do not remove This will allow adjustment of the lower section of the door Adjust and tighten as necessary Do not use excessive torque on these nuts b Loosen the two nuts near the base of the upper door hinge See Figure 15 This will allow adjustment of the upper section of the door Adjust and tighten as necessary Again do not use excessive torque on these nuts 13 Strip the ends of the door heater wires about 3 8 inch Connect one black wire from the door heater to one of the cut black wires with a wire nut Repeat this for the other set of black wires with another wire nut Connect the 2 white wires together with another wire nut Finally attach the 2 ground wires together wit
27. constant ambient temperature and no drafts All samples should be placed in identical tubes filled to equal levels no higher than top of block All sample tubes should be placed in identical blocks MAINTENANCE These dry baths require no periodic maintenance other than routine cleaning with mild cleaning products Be sure units are unplugged prior to cleaning Never use abrasives or harsh chemicals to clean units Never immerse units in liquids or pour liquids onto the dry block support plate SERVICE It is recommended that dry baths be serviced only by those qualified in instrument maintenance Service is available at any of the Instrument Service District Offices located throughout the country If you need service or service information please contact the office nearest to you The representatives there can issue a return authorization number if necessary Emergency on site service calls are not covered under the warranty Unit Disassembly WARNING Unplug unit prior to disassembly 1 Loosen set screws and remove control knobs 2 Turn unit upside down and remove four screws 3 Remove base by first lifting at rear then moving forward to clear temperature control stems 4 Note the routing of wires and the location of insulation so that the unit can be properly re assembled 5 Carefully remove insulation Thermostat Replacement 1 Optional Remove screws that attach Heater Base Assembly to
28. is used on Model 5H or 10H Pin 1 is the sensing connection Pin 2 is analog ground Pin 3 is the reference connection When the T C type CO sensor is used the jumper located between components R 1 and C 3 must be configured for the T C position See Figure 3 Connector J 12 is an optional output for connection to a temperature recorder for the sole purpose of monitoring the chamber temperature output is 10 mv C The tip connection is positive The mating plug can be purchased through many electronic supply stores The plug is manufactured by Switchcraft Switchcraft P N 850 Connector J 13 is also an optional connection for monitoring the incubator through a central monitoring system Any alarm condition will activate relay K 2 thereby alerting the central monitor J 13 is 3 conductorjack This enables configuration either as NORMALLY CLOSED or NORMALLY OPEN as required by the monitoring system The case of J 13 is ground The tip connection is NORMALLY CLOSED Again the mating plug can be purchased through many electronic supply stores The plug is manufactured by Switchcraft Switchcraft P N 260 Connector J 14 is used for connection to the relative humidity sensor Pin 1 is the unregulated DC source Pin 2 is theoutput from te relative humidity sensor Pin 3 is analog ground 2 3 1 POWER BOARD CALIBRATION Normally the power circuit board is calibrated at the factory If however you find that th
29. mv C In theory it a thermometer reading in the center of the chamber reads 37 0 degrees The voltage at Pins 2 amp 3 should be about 370 millivolts You will probably find the output of the sensor slightly higher than this due the slightly elevated temperature of the fan motor plate if the above conditions are not met then replace the temperature sensor Refer to Section 3 5 for temperature sensor replacement 2 12 TIC CO SENSOR The CO sensor is a matched set of thermistor beads operating at an elevated temperature See Figures 3 amp 6 One side of the sensor reference section is hermetically sealed and senses only the chamber temperature The other side is exposed to both chamber temperature and atmosphere The exposed section with changes to CO2 concentration relative humidity and temperature Any sudden change In any of those parameters will cause the sensor to rapidly react to the change Increasing humidity will cause the CO display to fall Conversely if humidity decreases the CO disptay will rise Also an increase decrease in temperature wil cause the display to rise or fall When the glass door is opened the rapid change in relative humidity may cause the CO display to rise contrary to what would be logical The change is momentary and will stabilize quickly When CO is introducing into the chamber The CO has an insulating effect on the exposed section of the sensor Thi
30. replacement remove the fan motor mouning backe and float switch These items will be used again 1 Replace water jacket chamber module in reverse order of steps 6 through 10 above 12 are adjustment points at the upper and lower door mounting brackets so that the outer door may be adjusted to close properly The outer door gasket should contact th incubator body four sides If the door needs adjustment proceed as follows a With a wrench loosen the two nuts near the base of the lower door hinge See Figure 14 Do not remove This will allow adjustment of the lower section of th door Adjst and tighten as necessary Do not use excessive torque on these nuts b Loosen the two nuts near the base of the upper door hinge See Figure 15 This will alow adjustment of the upper section of the door Ajust and tighten as necessary Again do not use excessive torque on these nuts 13 Strip the ends of the door heater wires about 3 8 inch Connect one black wire from the door heater to one of the cut black wires with a wire nut Repeat this for the other set of black wires with another wire nut Connect the 2 white wires together with another wire nut Finally attach the 2 ground wires together with a wire nut Secure the wires from phsical damage 14 Complete by reversing steps 1 through 4 of the above procedure 3 16 FUSES 1 Disconnect electrical service 2 Pry the fuse holder out of the EM
31. the water jacket cartridge heaters and door heater Pins 1 amp 2 supply the left cartridge heater Pins 3 amp 4 supply the right cartridge heater Pins 5 6 7 amp 8 supply the door heater with Pin 8 being ground The connections are configured using the two voltage select jumpers located above Connector J 3 Connector J 4 supplies controlled output to the air heater through Pins 5 amp 6 J 4 also supplies power to the fan motor through Pins 4 7 amp 8 with Pin 4 being ground for the fan motor plate Pins 1 2 amp 3 are generally not used except for an optional electrical outlet located inside the chamber Connector J 5 15 the last AC connector Itis used for connection of the auxiliary chamber heater Pin 1 is common Connector J 6 is actually a pair of wires that are soldered in place which supplies 5 volts DC to the CO solenoid valve Pin 1 is the positive terminal Pin 2 is the negative terminal The solenoid valve itself however is not polarity dependent Connector J 7 connects the water jacket float level switch into the circuit The float switch is connected in series with an LED on the controller board through the power circuit board to give an indication of the water level in the water jacket Connector J 8 is used for interfacing the Infra Red type CO sensor circuitry used on Model 51H or 101H An interface board is connected here Pin 1 the unregulated DC source of the interface board
32. unit until the new cable appears 12 Follow the instructions supplied with the door replacement kit for proper use of the instal lation tools for the 20 pin connector Plug the connector back into J 9 on the Power board Attach the ground wire to the ground stud 13 the lower fill siphon port cover being sure to reconnect the sample tubing Re place the rear cover Replace the controller 14 Test the unit for normal control function 3 14 3 DUAL CHAMBER UNITS UPPER DOOR 1 Disconnect electrical service Remove contents from incubator chamber Refer to Section 3 17 on controller removal Remove rear cover Disconnect Connector J 9 from Power board Remove the controller cable ground wire from the ground stud Remove the screws from the top cover Remove 2 screws from each end of the fill siphon port cover See Figure 12 Slide the top cover forward about 1 2 inch to release it from the front of the enclosure Lift top up and disconnect the sample port tubing Remove top from unit Remove 2 screws from each end of the lower fill sipnon port cover See Figure 13 Grasp each end of the cover and push it down to release the front edge from the upper splash cover Lift cover out and remove sample port tubing Cut the 4 heater wires including ground about 3 inches below the lower door support for the upper chamber See Figure 13 These wires will be used again Cut the controller cable near the uppe
33. 20 289114 2 Blower Duct Air 2 2 2422222 102625010 1 101432011 1 Brolo i 247426 1 Air 24043 1 Cartrldge 143304100 2 Chamber Heater Outer 247423 1 E T 223720 1 Solenoid Valve ee 297047 1 Limiting Thermostat enssins 989490 1 Power Transformer 105039087 1 CO Sensor PC Board Assembly 102204101 1 Temperature Sensor 0 105028031 1 BIOWeFVVRGe 1402 020 1 e 102660011 12 Filter 52 102019009250 1 CO Filter internal 31012822222 1 Power Board 00374701 1 Control Panel Assembly
34. 44010 10 Water Fil HOSE 5 108001004 1 Gas 542875 2220 0000 1 Phone Plug 1 4 1418 040 2 Humidity Tray 102633001 2 32 REPLACEMENT PARTS LISTS MODEL 10H 110 120 VOLTS 50 60 Hz DESCRIPTION PART NO STD QTY 200 241077202680 4 Power Board Fuse 3 8 101408009 2 Fuse 5 AMP 5 X 20MM Ne 2891714 2 Blower Duct Air Plenum 22221 1 22 102625010 2 101432021205 1 247426 2 24743 2 Cartridge Heater 143304100 4 Chamber Heater Outer 24742304 2 alan tee 223720044408 2 Solenoid 2 2 224040044440 0 0 0 0 0 110 ENEA 297047 2 Limiting Thermostat 239190 2 Power
35. 8031 1 Blower Whee 1402 020 1 Shelf SUGGS 102660011 12 Filter External 102013005 1 CO Filter Internal 310128 1 Power Board Assembly 00374701 1 Control Board Assembly 2 22 2 2 00374902 1 21899 330260 1 Glass Door 2 11 107002003 1 Float Switch 2 2 2 1 105013039 1 Power 3530265 040 00 04 1 102644010 5 Water Fi HOSE 108001004 1 HEPA Gas 542575 1 Phone Plug 1 4 CMS 1418 040 1 102633001 1 30 REPLACEMENT PARTS LIST MODEL 51H 110 120 VOLTS 50 60 Hz DESCRIPTION PART NO STD QTY Foot Levelei 241077 4 Power Board Fuse 3 8 101408009 1 Fuse 5 5 X
36. ANUAL FOR FISHER AUTOMATIC CO WATER JACKETED INCUBATORS Models covered by this manual 116875 1168710 116875H 1168710H 1168751 11687101 1168751H 11687101H NOTICE THE MATERIAL IN THIS MANUAL IS FOR INFORMATION PURPOSES ONLY THE CONTENTS AND THE PRODUCT IT DESCRIBES ARE SUBJECT TO CHANGE WITHOUT NOTICE FISHER SCIENTIFIC MAKES NO REPRESENTATIONS OR WARRANTIES WITH RESPECT TO THIS MANUAL IN NO EVENT SHALL FISHER SCIENTIFIC BE LIABLE FOR ANY DAMAGES DIRECT OR INCIDENTAL ARISING OUT OF OR RELATED TO THE USE OF THIS MANUAL FISHER SCIENTIFIC CORPORATE HEADQUARTERS 711 FORBES AVENUE PITTSBURGH PA 15219 REV D DATED 9 11 95 MANUAL 512S AZ 4 SERVICE MANUAL TABLE OF CONTENTS SECTION PAGE INTRODUC TION 1 1 11 DESCRIPTION OF SYSTEM 2 4 1 1 1 2 TROUBLESHOOTING THE INCUBATOR SYSTEM 4 13 CALIBRATION as testi cate 6 1 3 1 TEMPERATURE DISPLAY 2 6 1 3 2 SAFETY SET POINT CALIBRATION 6 1 3 3 RELATIVE HUMIDITY CALIBRATION 7 18 4 CALIBRATION 7 1 3 5 THERMOCONDUCTIVE CO SENSORS 8 1 26 GAS SAMPLES 8 2 TEST PROCEDURES 9 2 1 POWER SWITCH EMI
37. Disconnect the CO2 sensor from the interface board 2 units Disconnect the ground wire from the fan motor plate Disconnect the CO2 injection and sample port tubing from the fan motor plate See Figs 3 6 amp 7 Remove the screws from the top cover Remove 2 screws from each end of the fill siphon port cover See Fig 14 Slide the top cover forward about 1 2 inch to release it from the front of the enclosure Lift top up and disconnect the sample port tubing Remove top from unit Remove the insulation from the top of the chamber Disconnect the auxiliary heater and float switch wires from the top of the chamber See Figure 5 Set the unit on its back Use 3 inch thick support blocks to rest the unit on Cut the 4 heater wires including ground about 3 inches below the lower door support See Figure 14 These wires will be used again Remove 4 nuts and bolts from the upper door support bracket See Figure 15 Lift bracket and door off unit Support the door so as not to damage the controller cable Set the door off to the left side of the unit to allow clearance for chamber removal Remove glass door Remove the bolts that secure the splash cover lower door support to the unit and remove See Figure 13 a ES a 23 9 Remove the nuts one left and one right which secure the chamber bottom to the unit s exterior body See Figure 24 10 Remove the water jacket
38. Figure 6 Rear View Cover Removed 38 100 WATT AIR HEATER SPACER FOR BLOWER DUCT Relative Humidity Sensor SENSOR UMITING THERMOSTAT INJECTION PORT TEMPERATURE SENSOR SAMPLE PORT EAER RUST EE BLOWER WHEEL STRAIGHT AND PARALLEL MOUNTING NUTS 4 WALL Figure 7 Fan Motor Piate MOUNTING SCREWS 3 SENSOR AND SHIELD IR TC INTERFACE BOARD VR CABLE AND CONNECTOR CABLE AND CONNECTOR FUSE CARTRIDGE HEATERS WARNING ISOLATE FROM CHASSIS GROUND Figure 8 Interface Board and Related Components 39 2 PULL ON SHELF SLIDE TO DISENGAGE 3 FRONT SHELF SUPPORTS CAN NOW BE FROM BLOWER DUCT REMOVED BY LIFTING UP ON SUPPORT FRONT SHELF SUPPORT SHELF SLIDE BLOWER DUCT 1 LIFT UP ON FRONT EDGE OF SHELF SUDE TO REMOVE BLOWER DUCT TURN BLOWER DUCT SO IT CAN BE REMOVED DIAGONALLY FROM CHAMBER AS SHOWN BEING CAREFUL NOT TO DAMAGE DOOR GASKET Figure 10 Blower Duct Removal 40 HORIZONTAL KEYWAY VERTICAL KEYWAY PLACE TOWARD BACK OF CHAMBER PLACE TOWARD FRONT OF CHAMBER Figure 11 Shelf Slide REMOVE PROTECTIVE CAP TOP COVER SLIDE COVER FORWARD TO RELEASE COVER FROM THIS EDGE FILL SIPHON PQRT COVER SAMPLE PORT Figure 12 Top Cover Removal 41 UPPER DOOR SUPPORT BRACKET FOR LOWER CHAMBER BEHIND COVER UPPER SPLASH COVER ALSO SERVES AS LOWER DOOR SUPPOR
39. I filter 3 Remove fuse s in question from fuse holder 4 Locate source of fuse failure before replacing fuse s 5 Replace with new fuse s as required 6 Press fuse holder back in place 3 17 CONTROLLER BOARD ASSEMBLY WARNING THE CONTROLLER CIRCUIT BOARD CAN BE EASILY DAMAGED BY STATIC ELECTRICITY OBSERVE ALL RULES CONCERNING THE PROPER HANDLING OF STATIC SENSITIVE DEVICES GROUND YOURSELF KNOWN EARTH GROUNDED METAL OBJECT IMMEDIATELY BEFORE HANDLING STATIC SENSITIVE EQUIPMENT 1 Disconnect electrical service 2 Open the door and remove 2 screws from the top of the control module Slide the controller up and at the same time press the locking tab in to release the panel Slide the controller out of the door See Figure 2 It is best to wrap lay the board s anti static bags when handling them out of the door Many components used are static sensitive and can be easily damaged 3 Before replacing the controller assembly check to see that jumper JP 1 is set to the OUT position JP 1 is located above U 2 Next jumper JP 2 on the controller must be configured properly for use with either T C type CO sensor Models 5 5H 10 or 10 or for use with an I R type CO sensor Models 51 51H 101 or 101H JP 2 is located above 26 U 2 Finally check that jumper JP 3 is set to the OUT position JP 3 is located to the left of U 2 See Figure 1 While holding the locking tab
40. J 11 from the Power board Remove the fan motor plate from the chamber 5 Disconnect heater wires at heater terminals See Figure 6 6 Remove two 2 nuts and washers from the 2 heater posts and remove heater from fan motor plate 7 Replace air heater in reverse of the above procedure Be sure heater is installed straight and parallel with chamber walls to avoid hot spots 3 10 DOOR HEATERS 1 Disconnect electrical service 2 Remove 6 screws which fasten door liner to door Raise inner edge of gasket to gain access to screws remove screws 4 completely from gasket this will aid in reassembly because screw tips will hold Remove door liner and at same time cut the 3 door heater wires about 8 inches above the lower door supports bracket These wires will be used again Carefully peel door heater from door liner to avoid wrinkling door liner Remove paper backing from new door heater and apply adhesive side to liner in same position with wires extending in same direction as faulty heater Be sure all air pockets are removed when applying heater to door liner This will prevent hot spots Cut the new heater wires to match the length of the original door heater wires Connect one black wire from the new door heater to one of the original black wires with a wire nut Repeat this for the other set of black wires with another wire nut Finally connect the 2 white wires together with a wire nut
41. N 45 PART 1 DESCRIPTION OF SYSTEM OPERATION TROUBLESHOOTING TEST PROCEDURES INTRODUCTION This manual covers the maintenance and servicing of the Model 5 10 51 and 101 series of Fisher automatic CO water jacketed incubators NOTE FOR THE REMAINDER OF THIS MANUAL AND TO SIMPLIFY UNIT DESIGNATIONS FISHER CATALOG NUMBERS WILL BE REFERRED TO BY THE FOLLOWING MODEL NUMBERS Catalog Model Number Number Description 116875 5 Single Chamber T C CO 116875H 5H Single Chamber T C CO RH 1168751 51 Single Chamber 1168751H 51H Single Chamber R CO RH 1168710 10 Double Chamber T C CO 1168710H 10 Double Chamber T C CO RH 11687101 101 Double Chamber 11687101H 101 Double Chamber This manual is divided into 4 basic parts Part One 1 deals with the description of system operation troubleshooting and calibration of the system Part Two 2 deals with test procedures Part Three 3 deals with removal replacement procedures and replacement parts lists Part Four 4 offers illustrations figures and schematics for troubleshooting the incubator It also offers a list of Fisher Scientific customer support centers and phone numbers 1 1 DESCRIPTION OF SYSTEM OPERATION The FISHER incubators described herein incorporate microprocessor based control systems in a modular chassis design All parameters for each chamber can be entered independently through the set point switch
42. NIT BE IN OPERATION FOR ABOUT 24 HOURS BEFORE ATTEMPTING CALIBRATION 1 3 1 TEMPERATURE DISPLAY CALIBRATION 1 An accurate thermometer should have been placed in the center of the incubator chamber previously Read the thermometer and compare it with the temperature display the display and the thermometer readings disagree open the outer door and remove two 2 screws from the top of the contro module See Figure 1 the control module up about three 3 inches until it locks in place see Figure 2 On the right side of the control module are four 4 potentiometers The top potentiometer R 8 15 used to adjust the temperature display Adjust R 8 until the display agrees with the thermometer inside the chamber Push the retainer in to release the control module Slide the control module back into the door Close the outer door 2 Allow the incubator to stabilize for a minimum of one 1 hour and check the temperature readings Recalibrate if necessary WARNING HANDLE MERCURY THERMOMETERS WITH THE UTMOST CARE MINUTE AMOUNTS OF MERCURY IN THE INCUBATOR FROM A BROKEN THERMOMETER MAY CAUSE DAMAGE TO THE CHAMBER BY ELECTROLYSIS AS WELL AS CREATING A TOXIC ENVIRONMENT WITHIN THE CHAMBER RENDERING THE INCUBATOR USELESS 1 3 2 SAFETY SET POINT CALIBRATION When the SAFETY SET button is pressed the TEMP C window displays the set point of the back up overtemperature control Normally this setting is between 0
43. NT WHILE UNIT IS PLUGGED INTO ELECTRICAL OUTLET REFER TO ENCLOSED SCHEMATICS AND ILLUSTRATIONS TO AID IN TESTS 2 1 POWER SWITCH EMI FILTER 1 Turn on power switch located on left side of unit next to the power cord 2 Check voltage across the EMI filter where the black and white wires are connected See Figure 6 3 If no power is indicated at the above connection disconnect the power cord and check the fuses inside the filter This is accomplished by prying the cover off the fuse holder section of the EMI filter The cover is located beneath the power switch The fuses can be visually checked or checked with an ohmmeter on the low resistance scale The resistance should be less than 0 2 ohm When replacing the fuse holder be sure the fuse holder orientation coincides with the electrical configuration of your unit This is accomplished by aligning the arrows on the fuse holder for the proper voltage WARNING CHANGING THE FUSE HOLDER VOLTAGE CONFIGURATION DOES NOT CHANGE THE OPERATING VOLTAGE OF THE UNIT DO NOT ATTEMPT TO USE THIS FOR THAT PURPOSE THE UNIT OPERATING VOLTAGE IS CONFIGURED AT THE FACTORY AT THE TIME OF ASSEMBLY 4 Connect the power cord to the EMI filter and turn on the power switch If voltage is still not present and the fuses are known to be good replace the EMI filter 5 If the fuses continue to open remove the black and white wires from the EMI filter Check the voltage at this c
44. OLTS 10 ADJUST 10 SENSOR 7 35 VOLTS 0 8 ADJUST T C 8 9 SENSOR NECTOR 2 11 RH SENSOR CONNECTOR 0 14 HEATER MOTOR CONNECTOR J 4 TEMPERATURE ECORDER J 12 MONITOR RELAY 2 CONTROLLER MONITORING CONNECTOR JACK 13 HEATER TRIAC 0 1 CONNECTOR 7 3 JUNCTION R 2JR AUXILLIARY CHAMBER EE HEATER CONNECTOR 4 5 POWER INPUT HEATER VOLTAGE J 8 CONNECTOR SELECTORS GROUND WIRE TO CHASSIS GROUND SOLENOID VALVE 9 2 Figure 3 Power Circuit Board UPPER DOOR SUPPORT WATER JACKET VENT PORT 108 OUTER DOOR GASKET DOOR LINER DOOR HEATER DOOR CONTROLLER CABLE DOOR HEATER WIRES DOOR GROUND INSULATION REMOVED FOR CLARITY LOWER DOOR SUPPORT Figure 4 Door Heater and Attaching Components 37 CO SAMPLE PORT TUBING AUXILIARY CHAMBER HEATER FLOAT SWITCH CONTROLLER CABLE UPPER DOOR SUPPORT BRACKET Figure 8 Top Cover Removed T C TYPE CO SENSOR CO INLET LIMITING THERMOSTAT AIR HEATER AIR HEATER TEMPERATURE SENSOR CONNECTOR J 10 TRANSFORMER TRARSFOANER TEMPERATURE SENSOR CONNECTOR 4 1 o EMI FILTER AUX HEATER CONNECTOR J 5 HEATER CONNECTOR J 3 A CO INLET INTERNAL CO FILTER Ake FAN MOTOR CO SOLENOID VALVE 9 CO SAMPLE PORT HEATER MOTOR CHAMBER CARTRIOGE HEATER CONNECTOR J 4 CO SENSOR CONNECTOR 11
45. Pin 2 is ground Pin 3 is the output from the CO sensor Pin 4 is analog ground Pin 5 is used for the shield When the R type CO sensor is used the jumper located between components R 1 and C 3 must be configured for the I R position 10 Connector J 9 is a 20 pin connector that connects the controller to the power circuit board The following table shows the pin designations CONNECTOR J 9 PIN DESIGNATIONS PIN NUMBER PIN DESIGNATION Tekin 5 VDC Digital Source Digital Ground 5 VDC Digital Source Digital Ground Solid State Relay Triac Solenoid Valve sures Safety Relay Alarm Relay Digital Ground Float Switch Virata Sensing Relative Humidity 1 Analog Ground Sensing T C Analog Ground 16 Reference T C AY Analog Ground Ae E Temperature Analog Ground Unregulated DC Connector J 10 is used for connection to the solid state temperature sensor Pin 1 is the 5 VDC source Pin 2 is the output from the temperature sensor Pin 3 is analog ground Connector 11 is used to connect thermo conductivity T C type CO sensor to the power circuit board The T C type CO sensor
46. T FOR UPPER CHAMBER PUSH COVER DOWN TO RELEASE THIS EOGE FROM UPPER SPLASH COVER FILL SIPHON PORT COVER Figure 13 Dual Chamber Units Fill Siphon Port Cover Removal OF DOOR SUPPORT DOOR SUPPORT ATTACHING BOLTS DOOR HEATER WIRES Figure 14 Lower Door Support NOTE DOOR ADJUSTMENT NUTS MAY BE LOCATED ON TOP 42 DOOR ADJUSTMENT NUTS DOOR ADJUSTMENT NUTS MAY BE LOCATED ON BOTTOM OF OOOR SUPPORT BRACKET CONTROLER CABLE DOOR SUPPORT BRACKET ATTACHMENT BOLTS UPPER DOOR SUPPORT BRACKET Figure 15 Upper Door Support Bracket 43 Oy OPTIONAL gt OPTIONAL WIRING DIAGRAM PIN 541950
47. This is a natural physical phenomenon common to all thermo Conductivity type CO sensing systems most users do not change thir operating relative humidity so this is not normally a concern The basic principle behind obtaining a diferent operating relative humidity is based on the amount of surface area of water provided by the humidity tray or dish There are other factors such as the integrity of the chamber seals etc Under stable temperature and relative humidity conditions FISHER s thermo conductivity type CO sensing systems rival Infra Red type sensing systems when comparing control accuracy Users with the desire or need to change their operating relative humidity frequently or for those who open and close the inner door many times on a daily basis which tends to lower the average relative humidity within the chamber will benefit the most from the Infra Red type CO2 sensing system models Models 51 51H 101 and 101H Infra Red CO2 sensing systems are not adversely affected by wide chanes in relative humidity 1 3 6 GAS SAMPLES Gas samples are taken with a suitable sampling instrument Bacharach Fyrite or Blood Gas Analyzer for example through the gas sample ports Part 2 TEST PROCEDURES NOTE VOLTAGE AND RESISTANCE MEASUREMENTS ARE TAKEN ACROSS THE PART UNDER TEST ALWAYS REMOVE POWER CORD FROM ELECTRICAL OUTLET WHEN REMOVING A WIRE FOR TESTS NEVER MAKE A RESISTANCE CONTINUITY MEASUREME
48. approximately 5 to 10 Maximum attainable temperature may exceed 140 C Temperature Range 0r 0 5C Variation at a given time of a sample temperature from the average of al sample temperatures within 22 dry block at 370 Temperature variation between blocks may exceed this spec Blocks Capacities 11 718 1 Block 11 718 2 2 Blocks 11 718 4 3 Blocks 11 718 6 4 Blocks 11 718 8 6 Blocks Power Requirements 11 718 115V 50 60 Hz 90 Watts 11 718 2 115 50 60 Hz 180 Watts 11 718 4 115V 50 60 Hz 270 Watts 11 718 6 1157 50 60 Hz 360 Watts 11 718 8 115 50 60 Hz 540 Watts Physical Data 11 718 X 8in X 3 5in 4 6 Ibs 11 7182 9in X 8in X 3 5in 4 7 Ibs 11 7184 QinX1linX3 5in 5 8 lbs 11 718 6 12 5in X 8 5in X 3 5in 6 1 lbs 11 718 8 12 5in X 1lin X 3 5in 7 4 Ibs Unpacking These Dry Bath Incubators are shipped in a single carton completely assembled Each is supplied with this manual a warranty card and a Dry Block Handle for handling or moving dry blocks The warranty card should be completed and returned as soon as possible In cases where shipping damage is observed keep the unit and carton intact including the packaging materials and file claim with the final carrier Note Because of the wide variety of applications and sample block designs dry blocks are not included with dry baths The user must select and order the blocks that best suit the part
49. ater from water jacket Refer to Section 3 17 on controller removal Remove rear cover Disconnect Connector J 9 from Power board Remove the controller cable ground wire from the ground stud See Figures 3 amp 6 Remove the screws from the top cover Remove 2 screws from each end of the fill siphon port cover See Figure 12 Slide the top cover forward about 1 2 inch to release it from the front of the enclosure Lift top up and disconnect the sample port tubing Remove top from unit Set the unit on its back Use 3 inch thick support blocks to rest the unit on Cut the 4 heater wires including ground about 3 inches below the lower door support These wires will be used again See Figure 14 Cut the controller cable near the upper door support bracket Cut this wire only if you are replacing the door Remove 4 nuts and bolts from the upper door support bracket See Figure 15 Lift bracket and door off unit Route new controller cable through upper door support bracket Route the door heater wires through the lower door support bracket 8 10 11 12 Place the door and upper door support bracket back on the unit Make sure door is square with unit Attach the upper door support bracket and tighten securely Cut the new door heater wires about 4 inches below the lower door support Strip the ends about 3 8 inch Connect one black wire from the new door heater to one of the ori
50. ator s manual Heating element s defective see Sections 2 5 26 amp 2 7 Defective Power circuit board see Setion 2 3 Fan motor defective see Section 2 8 Tempreature sensor defective see Section 2 11 Float switch defective see Section 2 10 R H calibration see Section 1 3 Defective R H sensor see Section 2 14 water supply depleted see operator s manual Defective door seals or fan motor plate gaskets repair and replace as necessary R H calibration see Section 1 3 1 3 CALIBRATION The calibration procedure is used to adjust the Temperature display Safety Set Point and CO parameters Read this section carefully before attempting to calibrate the unit Follow this procedure for the most accurate calibration CO must be calibrated last for best accuracy It is assumed that the incubator is to be operating at 37 0 C You can however operate at any desired temperature from 5 C above ambient to 50 0 C Prior to calibration the CO supply should be shut off Be sure the CO set point is set for 00 0 Place an accurate thermometer in the center of the chamber so it can be read with the glass door closed Now the door must be CLOSED for a minimum of one 1 hour before performing the calibration procedure IF THE INCUBATOR IS BEING OPERATED FOR THE FIRST TIME OR HAS BEEN OUT OF SERVICE FOR SOME TIME THEN THE INCUBATOR TEMPERATURE MUST HAVE A CHANCE TO STABILIZE WE RECOMMEND THE U
51. cover forward about 1 2 inch to release it from the front of the enclosure Lift top up and disconnect the sample port ubing Remove top from theunit Forthe lower chamber remove 2 screws from each end of the lower fill siphon port cover See Figure 13 Grasp each end of the cover and push it down to release the front edge from the upper splash cover Lift cover out and remove sample port tubing Remove wire nuts from the float switch wires With a thin flat blade screw driver or knofe carefully pry the float switch mounting bushing from the top of the chamber Once the bushing has come loose lift the float switch out from the top of the chamber See Figure 5 Remove paper backing from the washer on the new float switch Replace the float level switch in the reverse of the above procedure 3 13 INNER GLASS DOOR Remove the four 4 acorn nuts securing the two 2 glass door hinges to chamber face Remove the glass door Replace glass door in the reverse of the above procedure 20 3 14 OUTER DOOR CAUTION BEFORE ATTEMPTING TO REPLACE AN OUTER DOOR READ THIS SECTION THOROUGHLY BE SURE YOU HAVE THE PROPER TOOLS TO CRIMP A NEW CONNECTOR ON THE CONTROLLER CABLE THE CONNECTOR IS A 20 CONDUCTOR DEVICE THE CRIMPING TOOLS WILL BE MADE AVAILABLE WITH THE DOOR REPLACEMENT KIT 3 14 1 SINGLE CHAMBER UNITS Disconnect electrical service Remove contents from incubator chamber Remove the shelves Drain w
52. e calibration is not correct follow these steps for proper calibration 1 Disconnect electrical service from unit 2 Disconnect harness Connectors J 9 and J 10 from the power circuit board See Figure 3 3 TC IR Jumper must be in the TC position for calibration purposes On Models 51 51H 101 and 101H reset jumper to IR position after calibration and checking Step 7 are complete 4 Attach a voltmeter across Pins 1 amp 3 Connector J 10 5 Connect electrical service to unit Adjust R 10 for an output of 5 1 volts The accuracy of this calibration is critical 6 Next remove the test lead from Pin 1 and attach it to the junction of R 2 R 3 right side of R 2 R 3 See Figure 3 This junction is 11 located near capacitor C 5 Leave the other test lead attached to Pin 3 on Connector J 10 7 Adjust R 9 for an output of 7 35 volts Again the accuracy of this calibration is critical 8 Disconnect the power to the unit Reconnect harness connectors J 9 amp J 10 to the power circuit board Apply power to the unit and recheck the calibration Adjust as necessary 9 If the above conditions cannot be met replace the power circuit board Refer to Section 3 18 for power circuit board replacement 2 4 CONTROLLER CIRCUIT BOARD The controller assembly located in the door consists of two 2 boards the digital display board and the CPU board These boards contain the digital displays set
53. e if found faulty Refer to Section 3 2 for solenoid valve replacement 2 10 FLOAT SWITCH The float switch monitors the water level of the water jacket See Figure 5 When the water level drops amount the float switch contacts close and allows the LOW WATER indicator to illuminate To test the float switch for a low water condition 1 Siphon a sufficient amount of water through the water fill siphon port About three liters or less should be sufficient The LOW WATER indicator should illuminate If not siphon another four liters of water If the LOW WATER indicator still does not illuminate replace the float switch To test the float switch for a high water condition 2 Add three liters of water to the siphon fill port The indicator should tum off If not ad another four liters of water Ad this water slowly for if the unit is overfilled the excess water will run out the vent tube on the upper face of the chamber See Figure 4 14 3 If the LOW WATER indicator does not turn off the float switch should be repaced Refer to Section 3 12 for float switch replacement 2 11 TEMPERATURE SENSOR The temperature sensor is a solid state device operating on 5 0 volts DC The input to the sensor be measured at Connector J 10 Pins 1 amp 3 the power circuit board See Figures 3 amp 6 The output of the sensor is measured between Pins 2 amp 3 The output should be about 10
54. e lower chamber See Figure 13 Lift bracket and door off unit Support the door so as not to damage the con trolier cable Set the door off to the left side of the unit to allow clearance for chamber removal Remove glass door Remove the bolts that secure the splash cover lower door support to the unit and remove See Figure 13 24 9 Remove the nuts one left and one right which secure the chamber bottom to the units exterior body See Figure 14 10 Remove the water jacket chamber module 11 by sliding chamber out of the front of the exte rior body These chambers are awkward to handle and heavy Another person to aid in removal is advised if returning chamber to factory for repairs or replacement remove the fan motor mounting bracket and float switch These items will be used again Replace water jacket chamber module in reverse order of steps 6 through 10 above 12 There are adjustment points at the upper and lower door mounting brackets so that the outer door may be adjusted to close properly The outer door gasket should contact the incubator body evenly on all four sides If the door needs adjustment proceed as follows a With a wrench loosen the two nuts near the base of the lower door hinge See Figure 14 Do not remove This will allow adjustment of the lower section of the door Adjust and tighten as necessary Do not use excessive torque on these nuts b Loosen the two nut
55. e unit Make sure door is square with unit Attach the upper door support bracket and tighten securely 9 Cut the new door heater wires about 4 inches below the lower door support Strip the ends about 3 8 inch Connect one black wire from the new door heater to one of the original black wires with a wire nut Repeat this for the other set of black wires with another wire nut Connect the 2 white wires together with another wire nut Finally attach the 2 ground wires together with a wire nut Secure the wires from physical damage 10 Set the unit upright There are adjustment points at the upper and lower door mounting brackets so that the outer door may be adjusted to close properly The outer door gasket should contact the incubator body evenly on all four sides If the door needs adjustment proceed as follows a With a wrench loosen the two nuts near the base of the lower door hinge See Figure 14 Do not remove This will allow adjustment of the lower section of the door Adjust and tighten as necessary Do not use excessive torque on these nuts b Loosen the two nuts near the base of the upper door hinge See Figure 15 This will allow adjustment of the upper section of the door Adjust and tighten as necessary Again do not use excessive torque on these nuts 1 attach the end of the new controller cable to the end of the old controller cable and pull the old cable out from the back of the
56. es on each door mounted controller The incubator is completely automatic easy to maintain and service in your laboratory The following is a description of the system and how it operates The control systems are housed in each door with signal conditioning and processing sent to a power board at the rear of the Incubator Most troubleshooting procedures for this system can be carried out with the rear covers removed and the controller pulled to the Service Position All electrical wires are identified and referenced to terminal connections in accordance with the schematic diagrams All components and tubing connections can also be examined The shelves shelf support panels and blower ducts are easy to removed from the chambers for cleaning With these assemblies removed the chamber walls can be cleaned without interference There are no hard to clean areas Each water jacket chamber is manufactured individually and mounted into the incubator chassis allowing easy removal and replacement of a damaged chamber The chamber modules have been conveniently fitted with a combination fill siphon port so the water jackets can be filled or emptied with the same hose connection The water in the jacket maintains a constant temperature source surrounding the chamber enhancing the incubator s ability to provide a uniform temperature throughout the chamber Al environmental parameters in the chambers are monitored and reg
57. ginal black wires with a wire nut Repeat this for the other set of black wires with another wire nut Connect the 2 white wires together with another wire nut Finally attach the 2 ground wires together with a wire nut Secure the wires from physical damage Set the unit upright There are adjustment points at the upper and lower door mounting brackets so that the outer door may be adjusted to close properly The outer door gasket should contact the incubator body evenly on all four sides If the door needs adjustment proceed as follows a With a wrench loosen the two nuts near the base of the lower door hinge See Figure 14 Do not remove This will allow adjustment of the lower section of the door Adjust and tighten as necessary Do not use excessive torque on these nuts b Loosen the two nuts near the base of the upper door hinge See Figure 15 This will allow adjustment of the upper section of the door Adjust and tighten as necessary Again do not use excessive torque on these nuts Securely attach the end of the new controller cable to the end of the old controller cable and pull the old cable out from the back of the unit until the new cable appears Follow the instructions supplied with the door replacement kit for proper use of the installation tools for the 20 pin connector Plug the connector back into J 9 on the Power board Attach the ground wire to the ground stud 21 13 14 Replace the top co
58. h a wire nut secure the wires from physi cal damage 14 Complete by reversing steps 1 through 4 of the above procedure 3 15 2 DUAL CHAMBER UNITS LOWER CHAMBER Disconnect electrical service Remove contents from incubator chamber Remove the shelves Drain water from water Jacket Refer to Section 3 1 on shelf support blower duct removal and remove supports and duct Remove rear cover Remove the cartridge heater wires from Connector J 3 Disconnect Connectors J 4 J 10 J 11 T C CO2 sensor units from Power board Disconnect the CO2 sensor from the interface board 2 units Disconnect the ground wire from the fan motor plate Disconnect the CO2 injection and sample port tubing from the fan motor plate See Figs 3 6 amp 7 Remove 2 screws from each end of the lower port cover See Figure 13 Grasp each end of the cover and push it down to release the front edge from the upper splash cover door support Lift cover out and remove sample port tubing Remove the insulation from the top of the chamber Disconnect the auxiliary heater and float switch wires from the top of the chamber See Figure 5 Set the unit on its back Use 3 inch thick support blocks to rest the unit on Cut the 4 heater wires including ground about 3 inches below the lower door support See Figure 14 These wires will be used again Remove 4 nuts and bolts from the upper door support bracket for th
59. icular application OPERATION Each dry bath is equipped with two temperature controls thermostats a selector switch and a red lensed lamp which indicates heater operation The lamp comes on when the controlling thermostat as selected by the switch closes to supply power to the heater Once the dry bath reaches thermal equilibrium the cycling of the lamp and the heater should continue on a regular basis In case of a malfunction such as a heater failure or thermostat stuck in the closed position the lamp will remain on Should the thermostat fail with the contacts open the light will remain off 1 Place the unit a flat level surface in an area free of drafts and temperature changes Note Drafts and ambient temperature changes will adversely affect constancy and uniformity 2 Make sure that bath cavity is free of foreign matter then place empty dry block s into bath cavity 3 Connect unit to an appropriate grounded power source as specified on the unit s data plate 4 Insert an immersion type thermometer graduated to the desired temperature range into the thermometer well in the dry block Note For more precise measurements the thermometer can be placed into the sample tube or a similar tube adjacent to the sample tube 5 Set the three position switch at the appropriate setting Low setting for ambient to 60 High setting for 50C to 130 6 Adjust the appropriate temperature con
60. inch to release it from the front of the enclosure Lift top up and disconnect the sample port tubing Remove top from unit Remove the insulation from the top of the chamber Disconnect the auxiliary heater and float switch wires from the top of the chamber See Figure 5 Remove 2 screws from each end of the lower fill siphon port cover See Figure 13 Grasp each end of the cover and push it down to release the front edge from the upper splash cover door support Lift cover out and remove sample port tubing Cut the 4 heater wires including ground about 3 inches below the lower door support for the upper chamber See Figure 13 These wires will be used again Remove 4 nuts and bolts from the upper door support bracket See Figure 15 Lift bracket and door off unit Support the door so as not to damage the contrller cable Set the door off to the left side of the unit to allow clearance for chamer removal Remove glass door 25 8 Remove the bolts that secure the splash door support to the unit and remove See Figure 13 9 Remove the nuts one left and one right which secure the chamber bottom to the unit s exterior body See Figure 14 10 Remove the water Jacket chamber module by sliding the chamber out of the front of the exterior body These chambers are awkward to handie and heavy Another person to aid in removal is advised If returning chamber to factory for repairs or
61. ment of the lower section of the door Adjust and tighten as necessary Do not use excessive torque on these nuts b Loosen the two nuts near the base of the upper door hinge See Figure 15 This will allow adjustment of the upper section of the door Adjust and tighten as necessary Again do not use excessive torque on these nuts attach the end of the new controller cable to the end of the old controller cable and pull the old cable out from the back of the unit until the new cable appears 4 12 Follow the instructions supplied with the door replacement kit for proper use of the instal lation tools for the 20 pin connector Plug the connector back into J 9 on the Power board Attach the ground wire to the ground stud 13 Replace the top cover being sure to re connect the sample tube Replace the lower fill si phon port cover being sure to re connect the sample tubing Replace the rear cover Re place the controller 14 Test the unit for normal control function 3 15 WATER JACKET CHAMBER MODULE 3 15 1 SINGLE CHAMBER UNITS 1 Disconnect electrical service Remove contents from incubator chamber Remove the shelves Drain water from water jacket Refer to Section 3 1 on shelf support blower duct removal and remove supports and duct Remove rear cover Remove the cartridge heater wires from Connector J 3 Disconnect Connectors J 4 J 10 J 11 T C CO2 sensor units from Power board
62. nit 1 If the sensor fails to operate check the fuse of the I R interface board For other problems related to the I R sensor contact your local Fisher Scientific service representative Refer to Section 3 4 for I R CO sensor replacement 2 14 RELATIVE HUMIDITY SENSOR The relative humidity sensor is a solid state device working on the principle of capacitance change for a change in relative humidity The input voltage 15 is derived from the unregulated DC supply The output voltage is 0 1 0 VDC depending on the level of relative humidity being sensed Refer to Figures 3 and 7 The input voltage can be measured between Pins 1 and 3 on connector J 14 on the power board Refer to Figure 3 The output voltage can be measured betwen Pins 2 and 3 If the display reads 85 0 relative humidity your voltage reading should be approximately 850 mV at Pins 2 and 3 Your actual voltage reading may be slightly lower due to the offset to compensate for the slightly higher temperature seen at the placement of the relative humidity sensor This higher temperature tends to cause the average sensor reading to be slightly lower than the average relative humidity as measured within the working area of the chamber The output is directly proportional to the relative humidity being sensed Remember this value is the raw sensor output not the offset compensated reading as displayed on the front panel the above values are no
63. oint and CO These procedures are described in Section 1 3 Do not attempt to adjust potentiometers R 15 R 16 17 R 18 other problems are suspected with the board itis highly recommended you contact your local Fisher Scientific representative for service suggestions The controller board is designed for easy removal and replacement Refer to Section 3 17 for controller board assembly replacement 2 5 CHAMBER HEATER There are four 4 heaters for each chamber consisting of two 2 cartridge heaters located in the bottom of the water jacket directly heating the water one 1 auxiliary chamber heater located on the top of the water jacket refer to Section 2 7 and one 1 air heater mounted to the fan motor plate which heats the atmosphere within the chamber The air heater is protected with a limiting thermostat which is mounted to the fan motor plate heaters are rated at 100 watts each with the exception of the auxiliary chamber heater its rating being 50 watts This yields a total of 350 watts heat capacity To test the cartridge heaters 1 Disconnect electrical service from unit 2 To provide access to cartridge heaters remove the rear cover which exposes the power circuit board See Figure 6 3 Remove Connector J 3 from the power circuit board See Figure 3 With an ohmmeter measure at connector J 3 between Terminals 1 and 2 then Terminals 3 and 4 The following resistance values should be met
64. onnection again If voltage is now present there is a short circuit in the unit short circuit would cause the fuse s to continually open This is why no voltage was present when the black and white wires were connected to the EMI filter 6 isolate a short circuit reconnect the wires to the EMI filter after removing power to unit Remove Connectors J 3 J 4 and the wires from Connector J 5 from the power circuit board one at a time 7 the power switch to the ON position When the fuse s stop opening you have isolated the short circuit the fuse s stop opening after removing connector J 3 look for a defective water jacket heater or door heater If they stop after removing J 4 look for a defective fan motor or air heater If they stop after removing the wires from J 5 look for a defective auxiliary heater Leave voltmeter connected across filter to monitor voltage v 8 Ifthe short circuit is still not isolated remove Connector J 2 from the circuit board If the fuse s stop opening the short circuit is on the power circuit board 2 2 TRANSFORMER The transformer located above the power circuit board provides isolated power for the secondary components See Figures 3 and 6 The primary of the transformer is multi taped to allow an input of 120 Volts The secondary is a center tapped design providing an isolated UL Class 2 rating of 9 5 Volts to the power circuit board The 3 8 Ampe
65. ontrols have been calibrated properly you will be finishing the calibration procedure by adjusting the Again we will assume the doors have been closed a minimum of one 1 hour and that the incubator is operating at the desired temperature and relative humidity Remember a tray of cool water placed in the chamber to provide humidity could take up to eight 8 hours before it reached the same temperature as the chamber There should not be any CO in the chamber since the gas supply has not been turned on yet and the CO set point is set to 00 0 NOTE MODELS 5 5H 10 AND 10H ARE SENSITIVE TO CHANGES IN OPERATING RELATIVE HUMIDITY THESE UNITS MUST BE CALIBRATED AT THE DESIRED OPERATING RELATIVE HUMIDITY KEEPING THE DOOR CLOSED A MINIMUM OF 1 HOUR WILL ASSURE THIS CONDITION 1 Open the outer door and pull the control module up about three 3 inches until it locks in place See Figure 1 On the right side of the control module are four 4 potentiometers The second potentiometer R 9 is used to adjust the CO display Adjust R 9 until the display reads 00 0 This will ZERO the display Push the retainer In to release the control module Slide the control module back into the door and close the outer door 2 Turn the CO supply ON Adjust the CO line pressure between 15 and 25 PSI 1 0 1 7 BAR Enter the desired CO level with the CO set point switches Allow the unit to stabilize for a minimum of one 1 h
66. our 3 Take a gas sample from the Gas Sample Port with CO measuring instrument The displayed reading and the actual reading from your CO measuring instrument should agree If not open the outer door and pull the control module up and re adjust potentiometer R 9 until the two readings agree 4 Push the retainer in to release the control module Replace the two 2 control module screws and close the door 1 3 5 THERMOCONDUCTIVE CO SENSORS A themoconductive TC CO Sensor consists of exposed and sealed sensing sections The sealed section of the sensor is isolated from ambient atmosphwere and is not affected by changes in CO concentration or relative humidity This section is used as a reference level The exposed section reacts with changes in CO concentration telative humidity and temperature Any sudden change in any of those parameters will cause the sensor to rapidly react to the change Increasing humidity will cause the CO display to fall Conversely if humidity decreases the CO display will rise Also an increase or decrease in temperaure will cause the display to rise or fall When the gass door is opened the rapid change in relative humidity may cause the CO display to rise contrary to what would be logical The change is momentary and will stabilize quickly If you change the operating relative humidity level for any reason on Models or 10H re calibration of the CO control system is required
67. point switches alarm indicating and regulating LED s The controller also houses the safety set point and silence switches The controller incorporates an Intel 8051 series micro controller with a 27C256 EPROM Since the controller uses mechanical switches for setting temperature and CO parameters a battery back up for memory retention is not required The edge connector fingers are designated the same as Connector J 9 on the power circuit board See Figures 1 amp 3 The even numbered fingers are located on the front side of the board the odd numbered fingers are on the back side Jumper 1 also located above U 2 selects the configuration for the type of CO sensing system that is used JP 2 would be set for the OUT position for T C units Models 51 51H 101 and 104 use the Infra Red CO sensor This jumper must be set properly for your type of CO sensor Jumper JP 3 located to the left of U 2 configures the relative humidity sensor to be in circuit on these models This jumper should always be set for the OUT position valid set point is not entered the controller will not allow the unit to heat inject CO or both Valid set points for temperature control are 0 1 to 50 0 Valid set points for CO control are 0 1 to 20 0 Naturally a set point of 0 0 for either temperature or CO turns off that function The only calibration required in the is adjusting the temperature display safety set p
68. r 247431 1 Cartridge Heater 143304100 2 Chamber Heater Outer Top 247423 1 223720 1 Solenoid 297047 1 Limiting 23919022 1 Power 105039087 1 Sensor Assembly 105028030 1 Temperature Sensor 105028031 1 Blower 1402 020 1 Shelf Slides 102660011 12 CO Filter 102013005 1 Filter 310128 22 2 1 Power Board Assembly 00374707 1 Control Board 8 00374902 1 Glass DOOP 930260 509 0 1 Glass Door Assembly Nb 107002003 1 Float Switch Assembly Nt 105013039 1 Power eatoni 858025 1 canines 102644010
69. r circuit board See Figure 3 Measure at Connector J 3 Terminals 5 and 6 then at Terminals 6 and 7 The following cold resistance values should be met 120 volt operation 320 ohms 10 0 cold resistance 2 If the above values are not met replace the door heater Refer to Section 3 10 for door heater replacement 2 7 AUXILIARY CHAMBER HEATER The auxiliary chamber heater provides 50 watts of heat to the top front edge of the chamber See Figure This reduces the possibility of condensation forming on the ceiling of the chamber when operating at elevated relative humidity This heater is provided with a limiting thermostat to limit the maximum temperature the heater can obtain The limiting thermostat is built into the heater To test the auxiliary heater 1 Disconnect electrical service from unit 2 Remove the 2 wires from Connector J 5 at the power circuit board See Figure 3 3 Measure the resistance across these 2 wires The following values should be met 120 volt operation 280 ohms 10 cold resistance 4 If youinitially obtain a reading of infinity open and the unit is warm wait a few moments in case the limiting thermostat has opened If after a few moments the above values are not met replace the auxiliary chamber heater Refer to Section 3 11 for instructions on auxiliary chamber heater replacement 2 8 FAN MOTOR The fan motor provides circulation of the chamber atmosphere Refer
70. r door support bracket for the upper chamber See Figure 15 Cut this wire only if you are replacing the door Remove 4 nuts and bolts from the upper door support bracket Lift bracket and door off unit Route new controller cable through upper door support bracket Route the door heater wires through the lower door support bracket Place the door and upper door support bracket back on the unit Make sure door is square with unit Attach the upper door support bracket and tighten securely Cut the new door heater wires about 4 inches below the lower door support Strip the ends about 3 8 inch Connect one black wire from the new door heater to one of the original black wires with a wire nut Repeat _ 22 this for the other set of black wires with another wire nut Connect the 2 white wires together with another wire nut Finally attach the 2 ground wires together with a wire nut Secure the wires from physical damage 10 There are adjustment points at the upper and lower door mounting brackets so that the outer door may be adjusted to close properly The outer door gasket should contact the incubator body evenly on four sides if the door needs adjustment proceed as follows a With a wrench loosen the two nuts near the base of the lower door hinge see Figure 14 Do not remove This will allow adjust
71. re fuse F 1 soldered to the power circuit board protects the transformer primary 1 If there isn t any power to the controller check the power circuit board fuse If it is open replace the fuse with the same type and rating See Figure 3 2 Ifthe fuse continues to open the transformer may either be shorted or a secondary component may be shorted To isolate the transformer from the secondary components remove the secondary wires from Connector 1 This will be two 2 red wires and 1 red yellow wire on the right end of Connector J 1 See Figure 3 3 If the fuse continues to open replace the transformer Refer to Section 3 19 for transformer replacement 2 3 POWER PRINTED CIRCUIT BOARD The power printed circuit board is located on the back of the unit behind the rear cover power circuit board interfaces with the controller board as as the CO sensor and solenoid valve temperature sensor and heaters All power enters the power circuit board which in tum powers or controls other components the unit Refer to Figure 3 Connector J 1 is the transformer connector Pins 1 through 3 are the secondary terminals Pin 2 being the center tap and Pin 6 for 120 Volts Pin 8 is the common terminal Connector J 2 is the primary power input to the circuit board Pin 1 being ground Pin 2 is the common and Pin 3 as the primary or Hot connection Connector J 3 supplies controlled output to
72. s Normally Closed or Normally Open There are Form C contacts used for the output of this jack Alarm conditions that will activate this jack are High Temperature Low or High CO concentrations and a safety set point adjusted below the desired operating set point 1 2 TROUBLESHOOTING THE INCUBATOR SYSTEM 1 much condensation on glass 1 Door heater faulty see Section 2 6 door 2 Low no voltage check connections and wiring to Power circuit board see Section 2 3 3 Fan motor defective see Section 2 8 2 2 not regulating 1 Check if CO2 supply tank is empty 2 Check CO2 tubing for leaks kinks bends or bad connections 3 Check 2 solenoid see Section 2 9 4 Defective Controller board see Section 2 4 5 7 35 volt supply not adjusted properly see Section 2 13 6 Fuse on I R interface board defective see Section 2 13 2 sensor defective see Section 2 12 2 13 Fan motor defective see Section 2 8 3 Displays not lighted 1 Power circuit board not adjusted properly or defective power circuit board see Section 2 3 2 Power circuit board fuse blown see Sections 2 2 amp 2 3 3 Transformer defective see Section 2 2 4 Displays show random data 1 Power circuit board not adjusted properly or defective power circuit board see Sectio 2 3 2 Defective controller board see Section 2 4 5 DC power 1 Check fuse F 1 on Power circuit board see Section 2 3 2 Check if there is AC power
73. s causes the exposed thermistor temperature to increase causing a voltage imbalance between the reference and sensing sections Which 1 compared by the analog circuitry 2 13 CO SENSOR The Infra Red I R type of CO sensor works on the principle of detecting the amount of I R energy absorbed by the An source is energized at pre determined intervals This source is passed through a special filter lens and then passed on an detector The amount of energy is measured by the analog circuitry See Figures 6 amp 8 When CO is introduced into the chamber some of this CO gas is passed through the optical path inside the CO sensor As the CO level increases the amount of energy absorbed Increases The CO sensor is not significantly affected by changes in operating relative humidity An interface board is used between the sensor and the power circuit board See Figure 8 Note that the lower right mounting hole of the interface board must be isolated from chassis ground There is a metal shield surrounding the CO sensor which protects the sensor from electrical or magnetic interference generated by the fan motor This shield must be in place for proper operation of the unit The small diameter tube leading out of the sensor must be kept plugged at all times for proper operation See Figure 8 Any CO that might enter through here will significantly upset the calibration of the u
74. s near the base of the upper door hinge See Figure 15 This will allow adjustment of the upper section of the door Adjust and tighten as necessary Again do not use excessive torque on these nuts 13 Strip the ends of the door heater wires about 3 8 inch Connect one black wire from the door heater to one of the cut black wires with a wire nut Repeat this for the other set of black wires with another wire nut Connect the 2 white wires together with another wire nut Finally attach the 2 ground wires together with a wire nut Secure the wires from phys ical damage 14 Complete by reversing steps 1 through 4 of the above procedure 2 6 7 3 15 3 DUAL CHAMBER UNITS UPPER CHAMBER Disconnect electrical service Remove contents from incubator chamber Remove the shelves Drain water from water jacket Refer to Section 3 1 on shelf support blower duct removal and remove supports and duct Remove rear cover Remove the cartridge heater wires from Connector J 3 Disconnect Connectors J 4 J 10 11 T C CO2 sensor units from Power board Disconnect the CO2 sensor from the interface board I R CO2 units Disconnect the ground wire from the fan motor plate Disconnect the CO2 injection and sample port tubing from the fan motor plate See Figs 3 6 amp 7 Remove the screws from the top cover Remove 2 screws from each end of the fill siphon port cover See Figure 12 Slide the top cover forward about 1 2
75. slide the controller into the door Be sure the guides on the control panel are engaged into the door properly Push the locking tab back toward the circuit board so that the controller can be engaged into the edge card connector The locking tab should now spring forward and be held captive by the stud on the rear of the control panel 5 Replace the controller screws 3 18 POWER CIRCUIT BOARD NOTE VOLTAGE SELECT JUMPERS FOR TRANSFORMER AND HEATERS MUST BE PROPERLY SELECTED BEFORE ENERGIZING POWER BOARD FAILURE TO DO SO MAY DAMAGE POWER BOARD AND OR CONTROLLER BOARD Disconnect electrical service 2 Remove rear cover 3 Disconnect Connectors J 1 J 2 J 3 J 4 J 8 J 9 J 10 amp J 11 from the power circuit board Remove the wires from Connectors 5 amp 7 Remove ground wire to chassis See Figures 3 amp 6 4 Remove the mounting screws from the power circuit board The power circuit board may now be removed from the unit 5 Select the proper transformer tap and voltage select taps on the Power board Select the appropriate CO sensor type used on your incubator by configuring the IR TC jumper Refer to Figure 3 6 Remove the CO solenoid and bracket assembly from the board It will be used on the replacement board 7 Replace power circuit board in reverse order of above procedure NOTE EVEN THOUGH REPLACEMENT POWER BOARDS ARE PRE CALIBRATED AT THE FACTORY IT 1S RECOMMENDED THE POWER BOARD
76. t met then replace the relative humidity sensor Refer to Section 3 6 for relative humidity sensor replacement PART 3 REMOVAL REPLACEMENT PROCEDURES CAUTION BEFORE PERFORMING THE FOLLOWING REMOVAL REPLACEMENT PROCEDURES DISCONNECT THE ELECTRICAL SERVICE FROM THE UNIT SHUT THE GAS SUPPLIES OFF BEFORE SERVICING SOLENOID VALVES AND RELATED FITTINGS 3 1 SHELF SUPPORTS amp BLOWER DUCT 4 Remove contents from incubator shelves and remove shelves 2 Turn power switch off 3 Remove the shelf slides from the shelf support brackets See Figure 9 4 Remove the front shelf supports by lifting up on the shelf support so that the keyhole will clear the button at the top and bottom of each support Pull the shelf support up off each button See Figure 9 5 Support the blower duct with one hand while removing the wingnut from the blower duct Tilt the top of the blower duct towards the front of the chamber 8 Remove the blower duct from the chamber The blower duct must be tumed to the left or right as it can not be removed by pulling it straight out See Figure 10 7 Install in the reverse order of above procedure Be sure to reinstall the plastic spacer onto the long stud near the fan before replacing the blower duct Failure to so will cause the fan to stop turning by over tightening of the wingnut 3 2 SOLENOID VALVES 1 Disconnect electrical service Remove the rear cover
77. the Top Cover Assembly 2 Observe wiring connections then remove two wires connected to appropriate thermostat 3 Remove fastener holding thermostat in place then remove thermostat 4 Connect wires to new thermostat and install 5 Re attach Heater Base Assembly if it has been removed 6 Position base onto unit and check alignment of thermostat shaft with center of hole in front of base Remove base and reposition thermostat as regumeg then tighten securely Heater Replacement 1 The heater is permanently bonded to the dry block support plate and can only be replaced as a pre assembled unit This assembly is called a Heater Base Assembly 2 Remove the fasteners which attach the thermostats to the dry biock support plate 3 Remove the fasteners that attach the dry block support plate to the dry bath top cover assembly 4 Locate and remove the splices at the end of the heater leads 5 Connect the new heater leads making sure that the connections are secure and properly insulated _ 6 Attach the new dry block support plate and thermostats see Thermostat Replacement section Selector Switch or Indicator Lamp Replacement 1 Note wiring connections of device to be replaced then remove wires 2 Squeeze tabs that hold device in place then push out through front of base 3 Install new device into appropriate hole in base 4 Reconnect wires Re Assembly 1 Position insulation over heater
78. the reference and sensing sections which is compared by the analog circuitry If the CO level sensed is below set point the control circuit will proportionally control the CO solenoid valve and meter small amounts of CO into the chamber as required Proportional control accounts for less deviation in the amount of CO fluctuation within the chamber Normally CO levels are affected by changes in relative humidity when using a thermal conductivity type CO sensor Care must taken to avoid wide changes in operating relative humidity levels in order to maintain good CO control accuracy Re calibration of the CO control will be necessary when changing to a different operating relative humidity The Infra Red type of CO sensor Model 51H or 101H works on the principle of detecting the amount of I R energy absorbed by the An source is energized at predetermined intervals This source is passed through a special filter lens and then passed on to an detector The amount of I R energy is measured by the analog circuitry When CO is introduced into the chamber some of this CO gas is passed through the optimal path inside the CO sensor As the CO level increases the amount of I R energy absorbed increases This amount of energy is measured and compared by the analog circuitry If the CO level is below set point the control circuit will proportionally contro the CO solenoid valve as before The
79. to Figures 6 amp 7 in the illustration section of this manual The motor is an induction type AC motor running at line voitage The rotation of the motor is counterclockwise as viewed from the rear of the incubator 13 The circulation of the atmosphere is vital for proper operation of the incubator Without proper circulation poor control of the environment may be experienced relating to problems with temperature and CO regulation The motor requires no servicing as it uses permanently lubricated ball bearings There is a metal plate which is used as an interference shield The metal plate is located between the fan motor and the I R CO sensor This shield is used to prevent electrical or magnetic interference from affecting the I R type CO sensor found on Models 51 51H 101 and 101H This plate and any attached grounding conductors is vital to proper operation of the unit Itis not used with the T C type CO sensor found on Models 5H and 10H 1 If a fan motor fails to operate try turning the motor shaft If it is binding check and make sure the blower wheel is not forced onto the motor shaft too far or that it is binding against the blower duct If the blower duct is removed for any reason be sure the spacer is installed the long stud before re installing See Figure 7 This will keep wing nut from being over tightened and causing the blower duct to bend and stop blower wheel 2 You should
80. to the AC input terminals of the power circuit board If not check EM filter fuses see Section 2 1 3 Power circuit board defective see Section 2 3 4 Transformer defective see Section 2 2 6 DC voltages below normal 1 Disconnect J 10 from power circuit board to see if Controller board is loading the power supply 2 Power circuit board calibration see Section 2 3 10 11 12 13 DC voltages below normal No power Temperature above set point and Hi temp indicator Temperature in chamber not the same as that displayed Temperature too low Low water indicator LED on and chamber known to be full Low water LED off and chamber known to be low on water Relative humidity display indication too high above 99 0 Relative humidity display indication appears too low Disconnect J 10 from power circuit board to see if Controller board is loading the power supply Power circuit board calibration see Section 2 3 Defective AC power switch EMI filter see Section 2 1 Fuse blown in EMi filter see Section 2 1 Defective power Defective temperature sensor see Section 2 11 Defective Controller board see Section 2 4 Temperature calibration see Section 1 3 Check temperature sensor connection Temerature sensor defective see Section 2 11 Fan motor defective see Section 2 8 Safety temperature contro set below temperature set point see oper
81. trol to a setting that approximates the desired temperature The following can be used as a general guideline On the Low temperature control a setting of 0 will be ambient 6 or 7 about 40 C and 10 about 60 C On the High temperature control a setting of 0 will heat samples to about 50 C 5 will heat to about 90 C and 10 about 130 C 7 As the dry bath is heating monitor the temperature reading on the thermometer and observe the indicator lamp If the thermometer reading exceeds the desired temperature reduce the temperature control setting When the lamp cycles on and off at regular intervals or flickers the unit has reached equilibrium If the dry bath is not at the desired temperature adjust the temperature control in the appropriate direction 8 Once the dry bath has stabilized at the desired temperature samples may be inserted into the blocks 9 Use the Dry Block Handle to remove blocks Be sure that the Dry Block Handle is securely engaged with the block before lifting it from the bath CAUTION Be sure dry bath is connected to only grounded power sources of the appropriate voltage Never heat levels Tightly capped sample tubes may burst when heated Use the Dry Block Handle to move the dry blocks Turn the Selector Switch to the Off position and unplug unit when not in use FOR BEST RESULTS Use Dry Bath Incubators in an area with a
82. uitry that controls the chamber heaters There are four 4 heaters for each chamber consisting of two cartridge heaters located in the bottom of the water jacket directly heating the water one auxiliary heater located on the exterior top of the chamber to eliminate ceiling condensation and one air heater mounted to the fan motor plate The air heater extends into the area enclosed by the fan blower duct at the rear of each chamber which heats the atmosphere within the chamber The circulating fan draws air in from the bottom of the chamber moves the air across the air heater and up the blower duct and then re enters the chamber at the top The CO percentage in each chamber is maintained independently by the electronic controls and either a thermo conductivity type CO sensor Model 5H or 10H or an Infra Red type CO sensor Model 51H or 101H The different types of CO sensors are explained below The thermo conductivity type CO sensor Model 5H or 10H consists of a matched set of thermistor beads operating at an elevated temperature One side of the sensor reference section is hermetically sealed and senses only the chamber temperature The other side is exposed to both chamber temperature and atmosphere When CO is introduced into the chamber the CO has an insulating effect on the exposed section of the sensor This causes the exposed thermistor temperature to increase causing a voltage imbalance between
83. ulated by microprocessor based electronic controls All sensors are located on the motor plate of each individual chamber as are the gas injection ports and air heaters The close proximity of the sensors to the control devices permits the system to maintain precise control over the chamber environment and efficient recovery of the environment after the door is opened Temperature control is achieved by means of a temperature sensor located on the motor plate which monitors the chamber temperature The primary control circuits provide proportional control to the heaters thereby eliminating temperature overshoot A safety temperature control also monitors the chamber temperature through the primary temperature sensor the unlikely event the primary temperature control circuit fails and tries to heat the chamber continuously the safety temperature contro will remove power from the chamber heaters thus eliminating away temperature condition When set properly the safety temperature control can easily limit the maximum chamber temperature to within 1 0 of the set point entered with the primary control The unit is equipped with heated exterior doors which remove a majority of the condensation from the inner glass doors This feature enables laboratory personnel to make a visual check of the incubator s contents without disturbing the incubator atmosphere The door heaters are controlled by the same circ
84. ver being sure to RECONNECT the sample tube Replace the rear cover Replace the controller Test the unit for normal control function 3 14 2 DUAL CHAMBER UNITS LOWER DOOR Disconnect electrical service Remove contents from incubator chambers Remove the shelves Drain water from water jackets Refer to Section 3 17 on controller removal Remove rear cover Disconnect Connector J 9 from Power board Remove the controller cable ground wire from the ground stud See Figures 3 amp 6 Remove 2 screws from each end of the lower fill siphon port cover See Figure 13 Grasp each end of the cover and push it down to release the front edge from the upper splash cover Lift cover out and remove sample port tubing Set the unit on it s back Use 3 inch thick support blocks to rest the unit on Cut the 4 heater wires including ground about 3 inches below the lower door support for the lower chamber See Figure 14 These wires will be used again Cut the controller near the upper door support bracket for the lower chamber Cut this wire only if you are replacing the door Remove 4 nuts and bolts from the upper door support bracket for the lower chamber See Figure 13 Lift bracket and door off unit Route new controller cable through upper door support bracket Route the door heater wires through the lower door support bracket Place the door and upper door support bracket back on th

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