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Finesse E+ and ME+ Operator Guide

1. 5 270 5R Note 1 The retraction distance is not included in the DISTANCE display To zero the distance press and hold down CLEAR until it goes to zero 4 3 5 COMBINED Display TRIM 10u COUNT 24 This display combines all of the above information in the one screen Press 5 270 5R NEXT to display it To clear both Count and Distance press CLEAR until both go to zero Count will clear before Distance WAX EMBEDDED SPECIMENS The desired medium either wax or resin is selected from the Material Select Menu in the User Setup Options This is described in detail in Appendix A paragraph A 3 5 Note that wax is the factory set medium 4 4 TOSETTHE KNIFE HOLDER FOR CUTTING SPECIMENS THIS INSTRUMENT USES SHARP KNIVES MAKE SURE YOU UNDERSTAND THE SAFE AND CORRECT METHODS OF USING AND HANDLING THEM It is important to adjust the Knife Holder to the correct angle before sectioning 48 A77510300GB Issue 3 4 431 TOSETTHE KNIFE HOLDER TO THE REQUIRED ANGLE Release the Knife Lock Lever and move the Blade Holder to the desired angle using the angle marking scale on the side of the Blade Holder for guidance a Set the angle to the zero mark on the scale on the side of the Knife Holder 4 4 2 RECOMMENDED KNIFE ANGLES FOR SOLID KNIVES AND PREMIER PLUS BLADES Mx35 Set the Knife Holder initially to an angle of 0 You can then adjust the Knife Angle more accurately to suit the Knife and speci
2. 13 When the Trim Wheel is turned away from you the Specimen Head moves away from the knife When it is turned towards you the Specimen Head moves towards the knife Section 5 5 explains the use of the Trim Wheel when trimming a specimen 2 5 4 ROCK The rock facility is a method of quickly and efficiently a advancing and trimming a specimen without turning the handwheel fully ROCK is used to enable and disable the rock feature of the Finesse microtome Turn the Handwheel until the specimen block is above the knife a The rock position is saved when ROCK is pressed b ME Only If a Cutting Sector is already defined the top of c the Cutting Sector will be used as the rock position When the rock feature has been selected the green LED lights d The amber LED oc lights when the Specimen Head is above the height at which the Specimen Head advances Press and hold ROCK again to turn off the rock feature The green LED will turn off Section 4 6 describes how the rock facility is used to trim a Specimen 2 5 5 MEMORY When the MEMORY button is pressed the current advance position of the Specimen Head is saved to allow you to automatically position the next specimen at the same place The green LED is lit to indicate that a position is saved Note Any previous memory position will be overwritten when a new position is saved 14 A77510300GB Issue 3 If the Specimen Head is at the m
3. 4 front of the instrument in the top right hand corner a 89 In an emergency push the Emergency Stop button until it locks into position This immediately stops all 5 motors To release the Emergency Stop button when the emergency is cleared turn the button in the direction of the arrows marked on it When the Emergency Stop button is released the display prompts the operator to press the NEXT button before the Finesse can be used again When NEXT is pressed the Specimen Head rewinds to the backstop position before reverting to normal operation 8 A77510300GB Issue 3 2 3 MANUAL CONTROLS 2 3 1 BRAKE ALWAYS APPLY THE BRAKE BEFORE ADJUSTING THE SPECIMEN CLAMP OR KNIFE HOLDER AND WHEN LEAVING THE UNIT UNATTENDED The Brake Lever b is located on the face of the Handwheel at the base of the handle a The brake prevents movement of the Specimen Head by locking the handwheel and can be engaged with the Handwheel in any position To apply the brake turn the Brake Lever b until it points to BRAKE on the label d There is a positive feel as the brake locates into position WARNING ME Only To select the brake on the Finesse ME the Brake Lever must be turned in an anti clockwise direction only WARNING ME Only Do not turn the Handwheel manually if the Brake Lever is set to MOTOR the Motor Drive system may be damaged T
4. 49 TO SET THE CUTTING SECTOR FOR MOTORISED CUTTING ME only WARNING ME Only Do not turn the Handwheel manually if the Brake Lever is set to MOTOR the Motor Drive system may be damaged Motorised cutting allows the operator to cut specimens evenly and smoothly at low speeds It is particularly useful when cutting large numbers of samples The Cutting Sector allows the operator to select a suitably slow speed for cutting a sample whilst automatically allowing a fast return stroke to produce good quality sections in the optimum time To set the Cutting Sector release the Handwheel brake and turn the Handwheel until the specimen is positioned above the knife a Press SECTOR once to capture the top of the a sector Turn the Handwheel until the specimen is just below the knife b Press SECTOR again to define the end position Note 1 The highest position is always saved as the start b position 2 Ifan end position for the Cutting Sector is not defined the bottom of the cutting stroke is automatically used S instead 53 A77510300GB Issue 3 3 If no sector is set the complete cutting stroke is taken as the Cutting Sector for the purposes of speed control The top LED lights c when the Specimen Head is above the Cutting Sector The lower LED lights d when the Specimen Head is below the Cutting Sector c Note secr n 7 Both LEDs are lit when the Specimen Head is within the Cutting Sector 7 d 2 Fl
5. The Specimen Head is at its limit of travel and cannot move any further The Handwheel position is unknown An attempt has been made to set the sector top position too close to the bottom of the cutting stroke No Handwheel motion has been detected after motorised cut has been requested Footswitch has been enabled Footswitch has not been enabled Footswitch or footswitch enable has been pressed before its operating cycle has been checked 1 1 Move the Specimen Head in the opposite direction Rotate the Handwheel for one complete turn without stopping Turn the Handwheel to a new position and try setting the sector top position again Check motor selection 2 Check Brake is not engaged No action required if Footswitch is required Press 77 if Footswitch not required Press 0 if Footswitch required Fully depress the Footswitch once and then carry on with normal operation The following screens appear for information on the Finesse ME only 65 SPECIFICATION AND ACCESSORIES 7 4 SPECIFICATION 7 1 1 Physical Width handle down 420 mm 16 ins Width handle down 480 mm 19 ins Depth 530 mm 21 ins Depth with connectors at rear 590 mm 23 ins Height 330 mm 13 ins Weight 40 kg 88 Ibs 7 1 2 Electrical Power Supply Voltages 100 120 V a c factory set 220 240 V a c Frequency 50 60Hz Power 150 VA Contact your Thermo dealer for details of a
6. adjust the T stud a by turning it anti clockwise to raise Blade the stud Guard To secure the Blade Holder to the Blade Holder Base turn the Knife Lock Lever in either direction until it locks Base Note Stage 1 The lock and release positions of the Knife Lock Lever Handle can be altered to suit the user s individual preferences Press the orange button and gently pull the Handle to release it Turn the handle to the desired position and release The Knife Lock Lever will work in the same way as before but the Handle position will be in the new position 2 If the Knife Lock Lever does not securely clamp the a Knife Holder adjust the T stud a by turning it clockwise to lower the stud TO SET THE REQUIRED ANGLE Release the Knife Lock Lever and move the Blade Holder to the desired angle using the angle marking scale on the side of the Blade Holder for guidance a Set the angle to the zero mark on the scale on the side of the Knife Holder A77510300GB Issue 3 35 It may be necessary to adjust the angle slightly for optimum results Each line marked on the scale corresponds to 1 To secure the Blade Holder in position turn the Knife Lock Lever until it locks TO SAVE THE BLADE HOLDER POSITION The angle setting of the Blade Holder can be saved If the Blade Holder is moved or refitted the previous angle can be easily achieved Note 1 The position can be saved on each individual Blade Ho
7. b Knife angle too steep or sharp c Specimen too hard for current conditions d Calcified areas in tissue e Knife blunt TABLE 2 PROBLEMS WHEN SECTIONING WAX EMBEDDED SPECIMENS SYMPTOM POSSIBLE CAUSE REMEDY 1 Ribbon of a The leading and trailing edges a Use a scalpel to trim the edges section is of the block are not parallel of the block until parallel curved b One area of the knife or blade b Sharpen the knife or use is blunt different part of blade c Surplus wax on one side of c Reduce excess wax block d Turn block through 90 and d Variation in consistency of cool with ice Mount sections specimen individually 2 Sections a Wax not hard enough for the a Use higher melting point wax or alternate conditions cool block with ice between thick b Block or knife is loose b Tighten Knife holder blade and thin clamp and orientation lock Clean Specimen Holder c Insufficient clearance angle c Slightly increase the angle d Sections of large hard tissue d Increase section thickness are too thin Use Fixed Head and solid knife to increase rigidity 3 Chatters a Block or knife loose in holder a Tighten block or knife Reduce angle to minimum with clearance Use heavy duty knife Reduce knife slant angle Soften tissue with softening fluids Re process re embed the specimen Decalcify the surface or dehydrate then decalcify Sharpen knife or change blade 4 Sections split a Nick in edge of knife
8. 3 2 1 TO UNPACK If the packaging has been damaged check the condition of the instrument Contact your dealer if there is any damage The bench where Finesse will be located should be rigid and level and made of a non flammable material It must be able to hold 40kg 88lbs and be large enough to provide good access to the Handwheel Remove the top layer of packaging from the Finesse Get help to lift the instrument from the box and to place it on the bench To lift or move the Finesse hold the Finesse securely at the lifting positions located under the front a and rear b of the instrument To remove the packing c from the Orientation Head support the weight of the Orientation Head and raise it slightly Remove the packing and carefully lower the Orientation Head to its lowest position 20 A77510300GB Issue 3 WARNING The Orientation Head is heavy Remove the Debris Tray Handwheel and accessories from the packaging and carefully place them on the bench WARNING The Handwheel is heavy Remove the two Orientation Head Adjustment Screws from the small bag provided and screw them into the orientation head d Make sure that you have received all the parts listed on the packing list supplied with the instrument Contact your Thermo dealer if necessary Note 1 Inform your Thermo dealer immediately if there are any breakages or shortages Quote the instrument Serial Number your Order Number Invoice Num
9. Sharpen knife or use different form a ribbon b Debris on knife edge c Knife angle too sharp or shallow d Uneven edges of block e Mould release or detergent ao or scored area of blade perpendicular b Specimen contains hard b If calcium deposits decalcify to the knife particles c Re embed using fresh filtered edge c Wax contains hard particles wax 5 Nojoining of a Wax too hard a Re embed in lower melting point sections to wax or breathe on block to soften WEAR MESH GLOVES and clean with rag moistened in wax Solvent Adjust to optimum angle Use scalpel to straighten leading or trailing edges until parallel Wipe around edge of block with absorbent paper 60 A77510300GB Issue 3 TABLE 2 continuation WAX PROBLEMS WHEN SECTIONING WAX EMBEDDED SPECIMENS 6 Sections attached to block on return stroke a Insufficient clearance angle b Debris on edge of knife Debris on edge of block a b Increase clearance angle WEAR MESH GLOVES and use cloth moistened in wax solvent and clean carefully Trim the edge of the block up instead of keeping flat on knife b Rake angle too small c Section thickness too large for type of wax Areas of a Incomplete impregnation of the a Return specimen to vacuum specimen in specimen impregnating bath for a few block not in hours or re process if the fault wax b Wax becoming detached from is exc
10. ii Do not modify the instrument if unauthorised modifications are carried out the instrument may be made unsafe and the warranty may be invalidated iii Potentially lethal voltages above 110V a c or 50V d c are present inside the instrument iv This instrument must be properly connected to a good earth Ground via the mains input supply v There are no user serviceable parts Do not remove any covers or panels 4 A77510300GB Issue 3 vi vii viii xi xii xiii xiv XV Finesse uses sharp knives Make sure that you understand the safe and correct methods of using and handling them BEFORE you use the instrument Always use the knife guards provided to cover the knife when the instrument is not cutting It is important that normal standards of safety and good laboratory practices are employed Always use common sense and the best known practice when operating the instrument The instrument weighs approximately 40 kilograms 88 Ibs get help to move or lift it If the instrument has been used with materials that are toxic or contaminated with pathogenic micro organisms follow the cleaning instructions given in Chapter 5 The Product Return Certificate found in Appendix B must be completed if the instrument is to be returned to Thermo The instrument should be regularly cleaned as described in Chapter 5 of this Operator Guide Use only factory approved accessories or replacement parts with Finesse Correc
11. unlock Move top stage to required positiion A77510300GB Issue 3 37 TO CLEAN THE DISPOSABLE BLADE HOLDER REMOVE THE BLADE BEFORE CLEANING THE BLADE HOLDER Pull the Clamp Lever towards you to loosen the clamp plate Drip xylene behind the clamp plate to loosen any wax build up a Remove any wax build up with a fine brush b Note sectioning 2 If thick thinning occurs reduce the clamping 3 If chattering of the blade occurs increase the clamping 3 4 7 GLASS KNIVES AND KNIFEHOLDERS INJURY ALWAYS HANDLE MICROTOME KNIVES WITH GREAT CARE MESH GLOVES PROVIDE SOME PROTECTION WHEN SHARP OBJECTS ARE HANDLED 1 MICROTOME KNIVES ARE EXTREMELY SHARP AND CAN CAUSE SERIOUS Ase ALL KNIVES IN THEIR TRANSPORT BOXES WHEN NOT REQUIRED FOR IMMEDIATE USE NEVER TOUCH THE CUTTING EDGE OF A GLASS KNIFE AS IT IS EASILY 1 ALWAYS WEAR SAFETY SPECTACLES WHEN USING GLASS KNIVES DAMAGED OR BLUNTED TO REMOVE DEBRIS USE A BLOWER 38 A77510300GB Issue 3 The Knife Holders for Ralph and triangular glass knives consist of two parts The base section is common to all of the glass Knife Holders and slots onto the fixed base in the same way as before see paragraph 3 4 4 TO FIT THE GLASS KNIFE HOLDER TOP SECTIONS The Knife Holder top sections are specific to the type of glass knife to be used Make sure that the Knife Holder is the correct type To fit the top section to the base section hold the top section uprig
12. Finesse e and place it in its packaging Att HANDWHEEL IS HEAVY TAKE CARE WHEN LIFTING IT Remove the two thumb screws from the Orientation Head g Place these in the small bag that contains the Handwheel plug screws and washer Support the weight of the Orientation Head and raise it to the mid point of its travel Place the packing piece h over the Orientation Head and position it as shown in the diagram Lower the Orientation Head so that it rests on the packing piece Note 1 The Orientation Head must be supported at the mid point of its travel Place the Knife Lock Lever Shaft small bag containing the Orientation Head thumbscrews and Handwheel plug screw and washer and Footswitch if applicable into the accessory box The Finesse is heavy so get help to lift it Hold the Finesse securely at the lifting positions located under the front and rear of the instrument i Lift the Finesse and place it on the bottom layer of packaging in the box Place the top layer of packaging on top of the instrument Place the Accessory Handwheel and Debris Tray boxes beside the Finesse in the packing case Fit the wooden crate over the top of the box see next page A77510300GB Issue 3 83 Thermo SCIENTIFIC RODUCT RETURN SAFETY DECLARATION Part1 DECONTAMINATION CERTIFICATE Any instrument or part of any instrument must b
13. Issue 3 3 4 5 SOLID KNIVES MICROTOME KNIVES ARE EXTREMELY SHARP AND CAN CAUSE SERIOUS INJURY ALWAYS HANDLE MICROTOME KNIVES WITH GREAT CARE MESH GLOVES PROVIDE SOME PROTECTION WHEN SHARP OBJECTS ARE HANDLED Ase ALL KNIVES IN THEIR TRANSPORT BOXES WHEN NOT REQUIRED FOR IMMEDIATE USE AN ie SOLID KNIVES LONGER THAN 180mm ARE USED AT THE EXTREMES THE KNIFE GUARDS WILL NOT PROTECT THE UNUSED CUTTING EDGE SOLID KNIFE HOLDER Turn the two elliptical Locking Knobs at the front of the Solid Knife Holder anti clockwise to release them Slide the knife into position under the Knife Clamp a and then tighten both Locking Knobs by turning them clockwise b Knife Guards are fitted as standard to cover the portions of the knife not in use and can be pushed together to cover the entire knife c Notes It is advisable to use a knife handle if available Knife lengths and profiles are shown in Appendix C Suitable knives are listed in the Thermo catalogue If the knife angle is changed the blade may move in relation to the section The Solid Knife holder is designed for use with Thermo knives However if a higher knife is to be used the Solid Knife holder can be adapted by moving the Knife Guard as follows A77510300GB Issue 3 33 Remove the screws a holding the Knife Guard to the rear of the Knife holder using the Allen key provided Position the Knife Guard in the higher position b and use the screws in
14. Setup facility described in Appendix A 2 The backlight is a very useful aid to align the knife to the specimen The Finesse will automatically enter the normal operation mode when the 5 button is pressed if it was left in the Standby mode Note If the Finesse has been switched off by using the O power switch follow the instructions in Section 3 4 1 The Finesse microtome is set at the factory for use with wax embedded specimens To change the desired medium to resin refer to the Material Select Menu options in User Setup described in Appendix A TRIM 10u The display will automatically show the TRIM screen when it is first switched on This is described fully in Section 4 3 The Finesse can be fully configured to the user s individual preferences The procedure is explained in the User Setup section in Appendix A 4 3 OPERATING DISPLAYS This section explains the different displays that will be encountered during normal use 4 3 1 TRIM Display Press TRIM SECTION on the Control Unit until TRIM is selected The top LED will light The Finesse automatically shows the TRIM display when it is has been set up This shows the thickness of material that will be removed during trimming 46 A77510300GB Issue 3 Note 1 Ifthe TRIM screen is not shown on the display press NEXT until it is The and buttons are used to increase and decrease the trim amount to the followin
15. The Footswitch remains active for a preset time If the Footswitch is not used within this time it will automatically be disabled The delay time can be altered as described in the Speed Options Menu section of the User Setup see Appendix A section A 3 5 1 8 A77510300GB Issue 3 3 The Footswitch works in the same way as the RUN button and depends on the cutting mode selected The normal position of the Footswitch is shown a To enable or disable the footswitch press the Footswitch Enable button on the Control Unit This is described in section 3 5 8 To cut press the Footswitch to Position 1 b The motor can be stopped at all times by fully depressing the Footswitch c or by pressing STOP on the Control Unit p E 1 b D SSS p A77510300GB Issue 3 19 INSTALLATION AND SETTING UP 3 1 INTRODUCTION The Finesse Microtome is a bench mounted instrument that must be unpacked and installed with care The maximum overall dimensions of the Finesse microtome are Width handle down 420 mm 16 ins Width handle up 480 mm 19 ins Depth 530 mm 21 ins with connectors at rear 590mm 23 ins Height 330 mm 13 ins FINESSE WEIGHS APPROXIMATELY 40kg 88lbs ALWAYS GET HELP TO SAFELY MOVE OR LIFT THE INSTRUMENT WITHOUT RISK OF INJURY 3 2 TO INSTALL THE INSTRUMENT
16. from the Knife and Knife Holder otherwise the success of sections may be impaired A77510300GB Issue 3 57 CLEANING AND MAINTENANCE 5 1 CLEANING A eemove THE KNIFE OR BLADE BEFORE ATTEMPTING ANY CLEANING OR MAINTENANCE OF THE MICROTOME Aw OFF THE POWER AND REMOVE THE MAINS PLUG FROM THE MAINS SUPPLY BEFORE CLEANING THE FINESSE Clean the instrument regularly as part of your normal housekeeping routine paying particular attention to the removal of wax and debris from the area around the Knife Holder Always remove the knife or blade apply the Brake and lock or remove the Knife Holder before cleaning or maintenance 5 1 1 To clean the Instrument To clean the outside of the Finesse wipe with a cloth damped in soapy water then polish with a dry cloth DO NOT USE EXCESSIVE LIQUID 5 1 2 70 clean the Knife Holder To clean the Knife Holder of residual wax remove it from its base by loosening the Knife Holder Lock then slide the Knife Holder towards you Use an appropriate wax removing solvent to clean away all traces of wax 5 1 3 70 clean the Disposable Blade Holder Pull the Clamp Lever towards you to loosen the clamp plate Drip xylene behind the clamp plate to loosen any wax build up a Remove any wax build up with a fine brush b 6 2 MAINTENANCE The Finesse Microtome requires annual lubrication by a qualified service engineer 58 A77510300GB Issue 3 TROUBLE SHOOTING 6 1 GENERAL Correct service and mai
17. stated but does not accept responsibility for any errors or omissions The development of Thermo products and services is continuous Make sure that any published information that you use for reference is up to date and relates to the status of the product If necessary check with Thermo or your local Thermo representative 2009 Thermo Fisher Scientific All rights reserved This manual may not in whole or in part be copied photocopied reproduced translated or converted to any electronic or machine readable form without prior written consent of Thermo 2 A77510300GB Issue 3 OPERATOR GUIDE LIST OF CONTENTS CONTENTS This Page WELCOME 4 Introduction Safety DESCRIPTION 7 Overview CONTROLS 8 Description Emergency Stop Manual Controls Front Panel Controls Control Unit Footswitch INSTALLATION AND SETTING UP 16 Introduction Installation Electrical Requirements Specimen Orientation Specimen Clamping Knife Holders Knives and Blades Sectioning procedure Wax and Resin Accessories OPERATION 36 Introduction Normal Operation Operating Displays Instructions for use for wax and resin embedded specimens CLEANING AND MAINTENANCE 48 Introduction Recommended Materials Routine Cleaning and Maintenance Instructions TROUBLE SHOOTING 59 Equipment Status Checks Problems when Sectioning Wax and Resin Warning Screens SPECIFICATION AND ACCESSORIES 62 WARRANTY STATEMENT 65 Warranty Statement Declaration of Conform
18. twice The first press will overwrite any existing memory position with a new position and the second press will clear the new memory position 4 8 TO CUT SECTIONS TRIM 9 ISECTIQN When trimming is complete select SECTION mode by pressing TRIM SECTION The lower LED will light Make sure that the specimen is positioned ready for cutting using the Trim Wheel and the Specimen Travel controls and v Sections can be cut manually using both models of the Finesse The ME can also cut sections using the motor 4 8 1 Manual cutting Press the or buttons to select the thickness required for the sections The values that can be selected are 0 5 3um in 0 5um steps 3 20um in 1um steps 20 40um in 5um steps 40 100um in 10um steps Note 1 In SECTION mode the retraction facility is selected automatically To turn it off press RETRACT Release the Brake and set it to MANUAL green position Turn the Handwheel Apply the Brake red position when sectioning is complete 52 A77510300GB Issue 3 4 8 2 Motorised cutting ME only To automatically cut sections select the mode required see paragraphs 3 5 10 to 3 5 13 by pressing MODE Release the Brake and set it to MOTOR blue position When RUN is pressed or the Footswitch operated the Finesse will section according to the mode it is in a single section in single cycle cutting or continuous sectioning in run mode or stop mode
19. 5 Note 1 Note that wax is the factory set medium Accessories such as a magnifier and cold light source are available to assist when resin embedded sections are to be cut Note It is recommended that the following points are followed when cutting resin embedded specimens 1 Usea Fixed Head 2 Select Retraction 3 Use motorised cutting as this provides the necessary control at low speeds to give smooth and even sections This option is only available on the Finesse ME 4 The motor will automatically select lower speeds 4 11 TO MOUNT A SPECIMEN The mounted specimen is held in a vice attached to the Fixed Head on the Finesse Section 3 4 contains full instructions of how to mount the specimen in the vice Note 1 Specimens must be securely clamped Clamp on the larger outer diameter of a JB4 or EBH holder to obtain better stability and rigidity A77510300GB Issue 3 55 4 12 TOTRIM A SPECIMEN The thickness required while trimming a specimen is set using the Front Panel and Control Unit see paragraphs 4 3 1 and 4 3 2 The v button can be used to advance a specimen to the knife Note When Resin is selected each click of the Trim Wheel is equal to the Trim distance set using the TRIM display paragraph 4 3 1 To trim using motorised cutting ME Only Set the Speed Control Wheel to a low speed Select the required cutting mode Release the brake engage the motor and press RUN Continue trimmin
20. Appendix A Section A 3 3 The facility can be turned off if not required The green LED on the RETRACT button on the front panel will be lit if the retraction option is selected o o RETRACT The operator can cancel the retraction selection by pressing RETRACT The green LED will switch off 1 0 A77510300GB Issue 3 2 4 FRONT PANEL CONTROLS The Main Control Panel is situated on the front of the instrument 2 4 1 DISPLAY The Finesse microtome has a backlit display to assist the operator More detail is given about each display in Section 5 3 2 4 2 AND and are used to step through the available options appropriate to the display The language display shows at initial power up When the F desired language appears on the display press NEXT to select it 2 4 3 NEXT NEXT is used to choose the next display in the sequence of displays and to select yey the desired option 24 4 CLEAR CLEAR clears the Count and Distance displays as applicable Hold down for 3 seconds If both Count and Distance are displayed press CLEAR for 3 seconds to clear the Count and then a further 3 seconds to clear the Distance A77510300GB Issue 3 11 2 4 5 RETRACT RETRACT selects and deselects the Retraction option for the Specimen Head LEDs on the button show the current status a b Green LED on b automatic retraction selected Amber LED on a specimen is full
21. ED ANGLE To help set the required cutting angle the Knife Holder Base is marked with a black engraved line The engraved line should be aligned with the scale engraved on the side of the Knife Holder Release the Knife Lock Lever and move the Knife Holder to the desired angle using the angle marking scale on the side of the Knife Holder for guidance a For the Thermo MB35 blade with a 35 facet angle set the angle to the zero mark on the scale on the side of the Knife Holder It may be necessary to adjust the angle slightly for optimum results Each line marked on the scale corresponds to 1 To secure the Knife Holder in position turn the Knife Lock Lever downward Note The angle marking scales are provided on solid and disposable Knife Holders only TO SAVE THE KNIFE HOLDER POSITION The angle setting of the Knife Holder can be saved If the Knife Holder is moved or refitted the previous angle can be easily achieved Note 1 The position can be saved on each individual Knife Holder only To save the angle set the Knife Holder to the required position and turn the Knife Lock Lever downwards to secure it in the desired position Use the Allen key provided to turn the screw in the side of the Knife Holder a anti clockwise to release it Slide the screw until you can feel the locking plunger locate b Tighten the screw To change the angle release the screw and repeat the above procedure 32 A77510300GB
22. EY SEQUENCE LANGUAGE E N G ENG ENGLISH USA ENGLISH USA FRE FRENCH GER GERMAN ITA ITALIAN SWE SWEDISH SPA SPANISH FIN FINNISH POR PORTUGUESE y DK DANISH NEXT CLEAR TO MATERIAL SELECT MENU Paragraph A 3 2 72 A77510300GB Issue 3 A 3 2 MATERIAL SELECT MENU This option allows the operator to specify whether the specimens being sectioned are embedded in wax or resin WAX is initially displayed If this is the required medium press NEXT otherwise press to display RESIN Press NEXT to select resin otherwise press either or to return to the wax option or press CLEAR to return to the Language Select Menu paragraph A 3 1 Note 1 The pre set selection is wax When NEXT is pressed the display will advance to show the Retract Amount Menu that is described fully in paragraph A 3 3 Flow chart A 3 2 shows the sequence of the Material Select Menu Note 1 To exit User Setup at any time press and release After a short pause the Finesse will exit to the TRIM or SECTION screens Flow Chart A 3 2 MATERIAL SELECT MENU FROM LANGUAGE SELECT MENU Paragraph A 3 1 NEXT CLEAR MATERIAL WAX for resin NEXT RESIN CLEAR TO RETRACT AMOUNT MENU Paragraph A 3 3 A77510300GB Issue 3 73 A 3 3 RETRACT AMOUNT MENU This option
23. Finesse E AND ME E AND ME ENGLISH OPERATOR GUIDE A77510300 A77510300GB Issue 3 SYMBOLS The following symbols and conventions are used throughout this manual and on the instrument THAT INSTRUCTIONS MUST BE FOLLOWED FOR SAFE AND CORRECT OPERATION IF THIS SYMBOL APPEARS ON THE INSTRUMENT ALWAYS REFER TOTHIS OPERATOR GUIDE 1 THIS SYMBOL IS USED ON THE EQUIPMENT OR IN A DOCUMENT TO WARN WARNING A warning is given in the document if there is a danger of personal injury or damage to samples or equipment Note 1 Notes give more information about a job or instruction but do not form part of the instruction Thermo Shandon Limited is an ISO 9001 and TickIT Accredited Company Finesse is a trade mark of Thermo Shandon Limited Thermo Fisher Scientific is the trading name of Thermo Shandon Limited Finesse E and ME meet the following CE Mark requirements C In Vitro Diagnostic Directive 98 79 EC 3P Low Voltage Directive 73 23 EEC as amended by 93 68 EEC c US All information contained in this manual is proprietary and confidential and the exclusive property of Thermo Fisher Scientific This manual is protected by copyright and any reproduction is prohibited This manual is for use only by the individuals to which it has been made available by Thermo Fisher Scientific Thermo Fisher Scientific makes every endeavour to ensure that the information contained in its support documentation is correct and clearly
24. H FIN FINNISH When the Finesse has been switched on turn the Handwheel for POR PORTUGUESE one complete rotation prior to use Apply the brake red position DK DANISH A77510300GB Issue 3 25 TO SWITCH OFF When the instrument is regularly used the Standby button should be used see 3 4 7 If the instrument is to be left unattended for long periods of time or is to be moved the power to the instrument should be turned off Press the O OFF side of the power switch s JJ to switch off the Finesse 3 4 SPECIMEN ORIENTATION ORIENTATION HEADS The Orientation Head enables a specimen to be aligned correctly to the knife A Fine Adjust Orientation Head is already fitted to the Finesse when it is supplied A Fixed Head must be ordered separately if required FINE ADJUST ORIENTATION HEAD AN eemove THE KNIFE AND KNIFE HOLDER FROM THE FINESSE BEFORE AN ORIENTATION HEAD IS FITTED The Fine Adjust Orientation Head allows the position of the a E specimen to be easily and accurately adjusted by the controls 6 N To fit the Fine Adjust Orientation Head apply the brake and 5 orientate the mounting plate as shown a Align the inner holes in the Mounting Plate b with those in the Specimen Advance Tube c Use the Allen key and the four short screws and washers provided d to secure the Mounting Plate to the Specimen Advance Tube Orientate the Fine Adjust Specimen Head with the Adj
25. IS FITTED A77510300GB Issue 3 27 The Fixed Head is securely bolted to the Specimen Advance Tube to ensure that the specimen is held extremely rigidly Note It is recommended that because of the extra stability that it provides the Fixed Head is used in the following situations 1 When resin embedded specimens are to be sectioned 2 When very thin sections of large hard tissue are to be taken the Fixed Head should be used in combination with a solid knife To fit the Fixed Head apply the brake and secure the Fixed Head to the specimen advance tube using the four washers screws and Allen key provided Use the four domed plugs to blank off the screw holes a Ve Tighten firmly using only the tools provided To attach the vices and specimen clamps to the Fixed Head first make sure that the Locking Collar is as far back as it will go on the Fixed Head turn it clockwise to check b Screw the vice or clamp as far as it will go onto the threaded stud protruding from the centre of the Fixed Head c Adjust the vice until it is in the desired orientation Use the spanner provided to turn the Locking Collar anticlockwise to tighten it against the vice d To rotate the head use the spanner to loosen the Locking Collar before rotating the vice or clamp to the desired orientation Secure the Locking Collar 3 4 58 SPECIMEN CLAMPING Specimens are normally embedded in wax or resin and then held in a cassette clamp or vic
26. Issued by K Waldron Quality Manager Thermo Fisher Scientific Anatomical Pathology Clinical Diagnostics Vee Ls uN Optional accessories considered subject to the In Vitro Diagnostic Directive IVDD are specifically indentified on this Declaration of Conformity Further supplies of standard accessories are treated as spares Convenience aids offered as accessories are not Subject to the IVDD A77510300GB Issue 3 69 Date 8 January 2010 APPENDIX A USER SETUP OPTIONS A 1 INTRODUCTION The Finesse can be configured to suit the medium being sectioned e g resin or wax and also to suit the user s individual preferences The flow chart in Section A 2 show the sequence of selections in the User Setup mode A reference is made to a paragraph number in Section A 3 in which more information is given about each option A 1 1 To enter User Setup the Finesse microtome must be in normal running mode with any of the TRIM or SECTION screens displayed Press and keeping this button pressed press MEMORY Next release c5 then release MEMORY The Finesse is now in User Setup mode A 1 2 To exit User Setup at any time press and release After a short pause the Finesse will exit to the TRIM or SECTION screens 70 A77510300GB Issue 3 A 2 OVERALL FLOW CHART OF USER SETUP SELECTIONS The following top level flowchart gives a reference to the relevant paragraph in Section A 3 where a full descri
27. OL UNIT IS CONNECTED OTHERWISE DAMAGE WILL OCCUR ME only To connect the Footswitch hold the round footswitch connector with the clip at the top as shown b Insert it into the top connector on the back panel until the clip clicks into place 24 A77510300GB Issue 3 3 4 TO SET UP THE INSTRUMENT It is important that the instrument is kept clean and free from wax and resin debris Make sure that the instrument is correctly set up as instability of the specimen cassette Specimen Clamp blade or Knife Holder could affect the quality of the sections AN sons APPLY THE HANDWHEEL BRAKE BEFORE ADJUSTING THE SPECIMEN CLAMP OR VICE 3 4 44 TO SWITCH ON AND OFF TO SWITCH ON Press the I ON side of the O power switch to switch the instrument on f Note The Specimen Head automatically moves back to its back stop position when power is applied to the Finesse Make sure that the screen shows a similar display to those a Fr INESSE shown in a and b before it finally displays the language selection screen shown in c b MICROTOME ME Ver x x 775 XXXX X Press to step through the language selections available on the LANGUAGE Finesse microtome The abbreviations are listed opposite d C for ENG USA d ENG ENGLISH UM USA ENGLISH USA When the required language is displayed press NEXT to select it FRE FRENCH GER GERMAN ITA ITALIAN SWESWEDISH SPA SPANIS
28. OME BLADES WITH GREAT CARE MESH GLOVES PROVIDE SOME PROTECTION WHEN SHARP OBJECTS ARE HANDLED i MICROTOME BLADES ARE EXTREMELY SHARP AND CAN CAUSE SERIOUS As ALL BLADES IN THEIR TRANSPORT BOXES WHEN NOT REQUIRED FOR IMMEDIATE USE ALWAYS WEAR SAFETY SPECTACLES WHEN USING GLASS KNIVES For best results use a glass knife and a Fixed Head Make sure that the Knife Knife Holder Fixed Head and vice are rigid and secure on the microtome A JB4 or EBH style resin specimen holder can be clamped on the outer diameter of the holder in the resin clamp to give better stability and rigidity for sectioning However the shaft of the holder can also be clamped Use spacers if necessary Insert the resin clamp into the vice held on the Fixed Head a and tighten the thumbscrew to secure the mount A77510300GB Issue 3 41 Make sure that the specimen is rigid and secure before sectioning Note 1 For best results the specimen and microtome must be properly set up before you begin to section a specimen Failure to do so may result in poor sections 3 7 ACCESSORIES MAGNIFIER 230V Europe only The Magnifier is a combined light and magnifying glass that attaches to the Finesse A separate Mounting Boss to attach the Magnifier to the Finesse and a new top tray are supplied To attach the Mounting Boss remove the top tray from the Finesse Remove the four screws d Align the holes in the Mounting Plate with the holes in the top of the F
29. OR Specimen travel opto failure Handwheel position sensor failure Control unit or control unit cable failure Fault detected at the Footswitch Press and release the Emergency Stop button then press NEXT to continue Call your Thermo agent if problem recurs Check Handwheel has been correctly installed Press and release the Emergency Stop button then press NEXT to continue Call your Thermo agent if problem recurs Press and release the Emergency Stop button then press NEXT to continue Switch off and wait 20 seconds Ensure that the control unit is connected correctly and that there is no damage to the cable Switch the unit on again Call your Thermo agent if problem recurs Press and release the Emergency Stop button then press NEXT to continue Turn the power off and check the connections before switching back on again Call your Thermo agent if problem recurs The following screens briefly appear for information When the message switches off the instrument can be used as normal 64 Refer to Appendix A to alter the screen speed A77510300GB Issue 3 TABLE 4 continuation WARNING SCREENS SCREEN acmon END OF TRAVEL TURN HANDWHEEL ONE TURN SECTOR TOP TOO LOW CUT ERROR Is motor engaged FOOTSWITCH ACTIVE FOOTSWITCH NOT ACTIVE PLEASE TEST FOOTSWITCH A77510300GB Issue 3
30. OUNT MENU Paragraph A 3 3 CLEAR NEXT DISPLAY OPTIONS to set NEXT DISPLAY HEADER SHOW NEXT DISPLAY COUNT SHOW NEXT CLEAR DISPLAY DISTANCE SHOW NEXT CLEAR TO SPEED OPTIONS MENU Paragraph A 3 5 DISPLAY DUALDISPLAY SHOW NEXT DISPLAY SPEED OMIT NEXT CLEAR A77510300GB Issue 3 77 A 3 5 SPEED OPTIONS MENU This menu allows the operator to specify the time the Footswitch is active the response speed of the screen and buttons The initial screen displayed is the Speed Options Menu Press to enter the different sub menus To advance to the Auto Load Options Menu paragraph A 3 6 without changing any speed options press NEXT or to return to the Display Options Menu paragraph A 3 4 press CLEAR If was pressed the first screen is the Footswitch Active display It is used to set the time allowed for the Footswitch to remain enabled if it is not used after it is first selected The pre set time is 30 seconds which means that the footswitch will stay enabled for 30 seconds if it is not used After 30 seconds it will automatically be disabled Press or to cycle through the different values shown below 2 4 6 8 10 15 20 30 40 50 60 90 120 180 seconds When the desired value is displayed press NEXT to select it and move
31. RAKE BEFORE THE KNIFE HOLDER IS REMOVED TO FIT THE KNIFE HOLDER TO THE BASE To fit the Knife Holder move the Knife Lock Lever on the right hand side of the instrument d until the T stud locking pin on the Knife Holder Base c is at its highest point Locate the T shaped cutout section on the bottom of the Knife Holder onto the T stud c Slide the Knife Holder onto the base making sure that the T stud slides along the cutout Note 1 The Knife Holder should not be too loose when fitted onto the base If it feels loose remove the Knife Holder and adjust the T stud c by turning it clockwise to lower the stud n 2 If the Knife Holder will not slide on at all or is very tight adjust the T stud c by turning it anti clockwise to raise the stud To secure the Knife Holder to the Knife Holder Base turn the Knife Lock Lever in either direction until it locks Note 1 The lock and release positions of the Knife Lock Lever Handle can be altered to suit the user s individual preferences Press the orange button and gently pull the Handle to release it Turn the handle to the desired position and release The Knife Lock Lever will work in the same way as before but the Handle position will be in the new position 2 If the Knife Lock Lever does not securely clamp the Knife Holder adjust the T stud c by turning it clockwise to lower the stud A77510300GB Issue 3 31 TO SET THE REQUIR
32. al lines across it Glass knife is loose in the holder Glass knife is blunt Resin block is too soft Tighten the Knife Holder Change the knife Harden the block if possible 3 Section has vertical Scores across it Glass knife edge is damaged Change the knife 4 Thin sections not successful Glass knife is blunt or damaged Resin block is soft Sectioning speed was too fast Change the knife Warm gently in an oven 40 50 C for a few hours to aid polymerisation or place in chamber over silica to harden the block Use a very slow speed for sectioning 62 A77510300GB Issue 3 TABLE 4 WARNING SCREENS SCREEN WEANING ACTION The following screens appear when instrument is first switched on for information CAUTION Settings may be lost if a Low Battery warning screen appears LOW BATTERY PRESS NEXT TO CONTINUE LOW BATTERY PRESS NEXT FOR DEFAULT PLEASE WAIT RECALCULATE 0 PROGRESS EMERGENCY STOP PRESSED Internal battery is losing its charge Any information set by the operator when the instrument was last used will be lost if the instrument had not been set to STANDBY before switching off the mains supply Internal battery is losing its charge All information set by the operator will be lost and the instrument will revert to factory set default values Instrument is calculating specimen advance control data base
33. allows the operator to specify the amount that the specimen head should retract from the knife on the return stroke The amounts displayed depend on whether wax or resin was selected in the Material Select Menu The values available for retraction are the same for both materials but their pre set values differ The values available are 5 10 15 20 25 30 35 40 60 80 100 125um Pre set value Wax Resin Note 1 Each material can be given a different value for the retract amount The pre set selection for wax is 5um The pre set selection for resin is 40um 2 If CLEAR is pressed and held down until the display is clear the option will always revert to the factory set pre set value If WAX was selected the pre set value of 5um will be displayed If this is the required amount press NEXT otherwise press or to advance through the other options If RESIN was selected the pre set value of 40um will be displayed If this is the required amount press NEXT otherwise press or to advance through the other options When the desired value is displayed press NEXT to select it otherwise press either or to advance through the other options again or press CLEAR to return to the Material Select Menu paragraph A 3 2 When NEXT is pressed the display will advance to show the Display Options Menu that is described fully in paragraph A 3 4 Flow chart A 3 3 shows the sequence of the Ret
34. ashing LEDs indicate that a position has not been set o SECTOR ol To cut a specimen select MOTOR on the Brake Lever blue position When RUN is pressed the Specimen Head will cut through the Cutting Sector at the speed set on the Speed Control Wheel At low cutting speeds the return speed is automatically faster than the cutting speed to save time Note 1 When the speed is being adjusted using the Speed Control Wheel the display will show the speed It will then revert back to the previous display The Display Option Menu section of the User Setup in Appendix A gives details of how to set the speed to be displayed permanently To clear the Cutting Sector press and hold SECTOR until both LEDs on the button clear 4 10 HOUSEKEEPING Always make sure that any debris is regularly cleared away from the Knife to ensure quality sections RESIN EMBEDDED SPECIMENS Resin embedded specimens require the use of specialised techniques Make sure that you are familiar with the necessary techniques and standard practices The following section gives instructions about how to use the Thermo Finesse to trim and section resin embedded specimens 54 A77510300GB Issue 3 THIS INSTRUMENT USES SHARP KNIVES MAKE SURE YOU UNDERSTAND THE SAFE AND CORRECT METHODS OF USING AND HANDLING THEM Select RESIN as the desired medium from the Material Select Menu in the User Setup Options This is described in detail in Appendix A paragraph A 3
35. ber Delivery Note Number and the date 2 If you ever need to transport the instrument refer to Appendix B for for repacking instructions 3 2 TO FIT THE HANDWHEEL The Handwheel must be fitted onto the Finesse when the instrument is first unpacked Carefully remove the Handwheel from its packaging WARNING Do not damage the encoder strip a on the Handwheel 74 me HANDWHEEL IS HEAVY TAKE CARE WHEN LIFTING IT Remove the screw washer plastic plug Alignment Shaft and 6mm A F Allen key from the small bag supplied Screw the Alignment Shaft into the centre of the hub and use a screwdriver or small coin to tighten it A77510300GB Issue 3 21 Make sure that the Brake Lever on the Handwheel is in the central gp TT position green position b N Note 1 Make sure that the Handwheel handle and the Specimen Head are at the bottom whenever the Handwheel is fitted or removed Carefully insert the Handwheel onto the Alignment Shaft on the right hand side of the instrument c until it touches the drive hub Move the Handwheel slightly backwards and forwards until the drive pin slots into position inside the Handwheel To check that the Handwheel is in position gently rotate it backwards and forwards If it is in the correct position the Specimen Head will move up and down Remove the Alignment Shaft from the centre of the Handwheel Put the washer onto the screw and fit into the centre of the Handwheel Tig
36. bles and disables the Footswitch The green LED lights when the Footswitch is enabled A77510300GB Issue 3 1 5 Note 1 The Footswitch must be fully depressed at least once after switching on the mains supply before it can be enabled Q Jg 2 The Footswitch remains active for a preset time If the Footswitch is not used within this time it will automatically be disabled The delay time can be altered as described in the Speed Options Menu section of the User Setup see Appendix A section A 3 5 2 5 9 SECTOR ME only SECTOR defines the Cutting Sector Turn the Handwheel until the specimen is above the knife b Press SECTOR once to capture the top of the sector Turn the Handwheel until the specimen is just below the knife c Press SECTOR again to define the end position Note 1 The highest specimen position is always saved as the start position 2 If an end position for the Cutting Sector is not Na e defined the bottom of the cutting stroke is i automatically used instead 3 If no sector is set the complete cutting stroke is Lo taken as the Cutting Sector for the purposes of speed control c The top LED lights e when the Specimen Head is above N the Cutting Sector The lower LED lights f when the i Specimen Head is below the Cutting Sector Note 1 Both LEDs are lit when the Specimen Head is within the f Cutting Sector Ey 2 Flashing LEDs indicate that a position has not been set o 0 The S
37. d for work with human or animal Transmissible Spongiform Encephalopathies e g Creutzfeld Jacob disease Scrapie or BSE YES NO If yes please contact Thermo Service before taking any further action Sigried E E E ods cue PIDPO O PERS p PpQpOUDDI POSHION uniesevediadeveuxes Part 2 Guidelines for Returning Instruments Please use the checklist below to ensure that the instrument being returned is ready for collection All reagents wax removed from instrument including vapour traps if applicable S Accessories are secured 91111590 Instrument has had transit clamps fitted as per operator 0111016 3 Instrument is packed in original packaging YES NO RMA NUMBERS corroe cts ar thd mi Rp A PAG cab teaads 4 E aaa PERRO MORE RORES CARRIER ws c2s cease oriea cud edgdad a dened ye Pa TNE dd MERGE ERR FOR ATTENTION ORD oie eter datae d tees cce bii Deo eiue fen dedo ue AA tn deben ordo ds Thermo Fisher Scientific Tudor Road Manor Park Runcorn WA7 1TA United Kingdom Tel 44 0 1928 534050 Fax 44 0 1928 534049 www thermo com pathology A77510300GB Issue 3 85 APPENDIX C KNIFE AND BLADE PROFILES C 1 INTRODUCTION This Section will provides useful information about knives and blades C 2 SOLID KNIVES The lengths of some com
38. d on values already set Emergency Stop button has been pressed Have battery replaced by a Thermo qualified Service Engineer Press amp to set the Finesse to STANDBY before switching off the mains supply Check that Trim and Section sizes are still correct before restarting Have battery replaced by a Thermo qualified Service Engineer Wait until finished the figure on the bottom row of the warning screen will count from 0 to 100 When the emergency has been cleared turn the Emergency Stop button in the direction of the arrows to release it The next screen will be displayed The following screen appears after the Emergency Stop has been pressed PRESS NEXT TO CONTINUE The Emergency Stop button has been released Press NEXT to continue The microtome will fully retract the advance assembly If the following screens appear press and release the Emergency Stop and press NEXT to continue If the same message appears again call your Thermo agent ADVANCE SYSTEM ERROR 1A A77510300GB Issue 3 Specimen travel motor or opto failure 1 Press and release the Emergency Stop button then press NEXT to continue 2 Call your Thermo agent if problem recurs 63 TABLE 4 continuation WARNING SCREENS SCREEN ACTION ADVANCE OPTO ERROR 1B HANDWHEEL ENCODER ERROR 1C CONTROL UNIT ERROR FOOTSWITCH ERR
39. e attached to the Orientation Head to section them 28 A77510300GB Issue 3 Instructions about attaching the specimen clamps and vices to the different Orientation Heads are given in Section 3 4 2 ALWAYS REMOVE THE KNIFE OR FIT THE KNIFE GUARDS OVER THE KNIFE BEFORE LOADING OR UNLOADING A SPECIMEN QUICK RELEASE CASSETTE CLAMP The Finesse Microtome uses a quick release cassette clamp that takes standard sized plastic specimen cassettes To fit a cassette into the cassette clamp apply the brake and push the quick release lever backward to slacken the lower jaw a N Fit the cassette either vertically b or horizontally c under the top jaw Let the quick release lever come forward d so that as the lever is released the jaws close under spring pressure to clamp the cassette in position The cassette clamp is interchangeable with the range of vices and holders detailed in the Thermo catalogue VICES The vice is operated by a thumbscrew that moves the upper jaw vertically to clamp the specimen against the lower jaw A resin clamp f and a number of spacer blocks one example is shown e are available that allow resin embedded specimens and small specimens to be sectioned These are positioned on the lower jaw of the vice Note The resin clamp must be used with the 50 x 45mm vice A77510300GB Issue 3 The two resin clamps are designed for a JB4 or EBH type resin specimen holder The m
40. e clean before being returned and where necessary accompanied by a completed Decontamination Certificate Should the instrument or any part of it be received in an unclean condition or Thermo Fisher Scientific consider it to be a hazard the instrument or part will be returned unrepaired at the expense of the customer It is important that the certificate is forwarded by post or fax and a copy attached to the exterior of the container Containers will not be opened until the company is in possession of the required certificate This form MUST be completed by the customer and NOT a Thermo or distributor employee If an instrument or part is to be returned to THERMO please note the following 1 If the instrument or any part of it has been exposed to or been in contact with potential pathogenic or radioactive material it is essential that it is decontaminated 2 Set procedures are laid down in the European Health and Safety Directives for decontamination To avoid any misunderstanding we request that all instruments or parts returned to us must be accompanied by a certificate stating the following We certify that this Model n Serial No has not been exposed to pathogenic radioactive or other hazardous material and has been cleaned OR has been decontaminated and cleaned if exposed to the above according to approved procedures following exposure to Has the instrument been use
41. ecimen positioning at the knife control unit that can be used on the microtome or on the bench removable debris tray tray for tool and specimen storage programmable rocking feature A77510300GB Issue 3 ME Only xv motorised cutting with speed indication xvi footswitch supplied for optional use The Finesse microtome can be used with the following knife holders in addition to a comprehensive range of accessories from our catalogue i solid knife holder ii disposable blade holder low and high profile variants iii triangular glass knife holder iv Ralph glass knife holders for 25 and 38mm knives Note Orientation Heads Knife Holders and Specimen Clamps should be ordered separately to individual preference Routine maintenance of the Finesse microtome requires only that the instrument is kept clean Note It is recommended that a glass knife and fixed head are used to section resin embedded specimens A77510300GB Issue 3 7 CONTROLS 2 1 DESCRIPTION This chapter describes the layout of the screen displays and the functions of all the controls on the instrument The Finesse has been designed for sectioning wax plastic and resin embedded specimens and general laboratory use Note ME Only 1 Motorised cutting is recommended when resin embedded sections are being cut 2 2 EMERGENCY STOP BUTTON Now USE THE EMERGENCY STOP BUTTON IN AN EMERGENCY The red Emergency Stop button a is situated on the
42. ed Options main screen and then press NEXT to go on to the Auto Load Options Menu paragraph A 3 6 Flow chart A 3 5 shows the sequence of the Speed Options Menu Note 1 If CLEAR is pressed and held down when the SPEED OPTIONS screen is displayed all three sub menus will revert to their factory set pre set values 2 If CLEAR is pressed and held down in any of the sub menus the individual option will always revert to the factory set pre set 3 To exit User Setup at any time press and release 5 After a short pause the Finesse will exit to the TRIM or SECTION screens Flow Chart A 3 5 SPEED OPTIONS MENU FROM DISPLAY OPTIONS MENU Paragraph A 3 4 NEXT CLEAR SPEED OPTIONS to set NEXT CLEAR FOOTSWITCH ACTIVE 30 seconds 2 seconds 120 180 CLEAR u im HIGH TO AUTO LOAD OPTIONS MENU MEDIUM Paragraph A 3 6 LOW SPEED BUTTONS HIGH MEDIUM H MEDIUM LOW A77510300GB Issue 3 79 A 3 6 AUTO LOAD OPTIONS MENU This menu allows the operator to specify the memory offset position to help with unloading the specimen and also whether to save or discard any rock positions that may have been set during operation The initial screen displayed is the Auto Load Options Menu Press to enter the different sub menus To advance to the Language Select Menu paragraph A 3 1 withou
43. emory position press MEMORY once to clear the memory position If the Specimen Head is not at the memory position press MEMORY twice The first press will overwrite any existing memory position with a new position and the second press will clear the new memory position 2 5 6 AUTO If the Specimen Head is at or in front of the position set in memory the Specimen Head will retract a set amount behind the memory position when AUTO is pressed The top amber LED will light a Note To alter the factory set offset distance of 5mm change the memory offset position of the Auto Load Option Menu in User Setup see Appendix A section A 3 6 If the Specimen Head is behind the memory position the Specimen Head will advance to the memory position when AUTO is pressed The bottom amber LED will light b Note If no position has been saved the Specimen Head will return to the back stop position but no LED will light when AUTO is pressed 2 5 7 TRIM SECTION and Operating Mode This button switches the display between TRIM and SECTION to show the Cc appropriate information When TRIM is selected the upper green LED will be SECTION lit c and the display will show the relevant TRIM display When SECTION is selected the lower green LED will be lit d and the display mi Wi will show the relevant SECTION display ce d 2 5 8 FOOTSWITCH ENABLE 0 ME only 1 ena
44. essive the Specimen Holder b Re attach with hot spatula Sections a Knife blunt a Sharpen knife or change blade excessively b Knife bevel too wide b Regrind or sharpen to produce compressed secondary narrow bevels c Wax not hard enough hot c Use higher melting point wax or specimen or ambient condi cool block with ice tions d Cut more slowly d Cutting too fast e Select by pressing RETRACT e Retraction not selected f Carefully drip xylene between f Wax debris has accumulated the clamp plate and backplate to behind the clamp plate of the remove the wax debris disposable Knife Holder Expansion a Poor specimen impregnation a Return specimen to a vacuum and disin impregnation bath for a few tegration of b Water temperature too high hours sections on b Cool water water surface 10 Sections coil a Knife blunt a Sharpen knife or change blade Reduce cutting angle by re sharpening or if clearance angle too small reduce the tilt of the knife Reduce the thickness of the section or use wax with a slightly higher melting point Raise temperature of the block by breathing on sections as they are cut A77510300GB Issue 3 61 RESIN TABLE 3 PROBLEMS WHEN SECTIONING RESIN EMBEDDED SPECIMENS SYMPTOM POSSIBLE CAUSE REMEDY 1 Knife howls Glass knife edge is damaged Change the knife as it passes Glass knife is loose in the Tighten the Knife Holder through the holder specimen 2 Section has horizont
45. g values 2 4 6 8 10 15 20 25 50 75 100 um Note 1 The thickness of the trim can be changed at any time during normal operation as long as TRIM is selected 4 3 2 SECTION Display Eton Press TRIM SECTION until SECTION is selected The bottom LED will light SECTION The screen will show the SECTION display which shows the thickness of 2 0u material that will be removed during sectioning The and buttons are used to increase and decrease the section amount to the following values 0 5 3um in 0 5um steps 3 20um in 1um steps 20 40um in 5um steps 40 100um in 10um steps Note 1 The thickness of the section can be changed at any time during normal operation as long as SECTION is selected 4 3 3 COUNT Display TRIM 10u The count increments with every cutting stroke To view this information 24 press NEXT until this screen is displayed To zero the count press and hold down CLEAR until it goes to zero A77510300GB Issue 3 47 4 3 4 DISTANCE Display SECTION 2 00 The Finesse keeps track of the total distance travelled To view this 5 270 5 information press NEXT again until the screen is displayed The value increments with every cutting stroke to show the overall distance travelled The R after the value signifies that the specimen head is retracted ection during the return stroke to the top of its travel 2 0u
46. g until the desired face of the specimen is presented to the knife Apply the handwheel brake when trimming is completed Note 1 The backlight is particularly useful when cutting resin sections 4 13 TO CUT SECTIONS Make sure that the Fixed Head Specimen Knife Holder and knife are all completely rigid before you start to section Select SECTION mode by pressing TRIM SECTION The lower LED will light p n To achieve accurate sections resin embedded specimens are best sectioned using motorised cutting as this allows the operator to cut specimens evenly and smoothly at low speeds Note When Resin is selected each click of the Trim Wheel is equal to the Section distance set using the SECTION display paragraph 4 3 2 56 A77510300GB Issue 3 To cut sections select the mode required see paragraphs 3 5 10 to 3 5 13 by pressing MODE Release the Brake and set it to MOTOR blue position When RUN is pressed the Finesse will section according to the mode it is in a single section in single cycle cutting or continuous sectioning in run mode or stop mode 4 14 TO SET THE CUTTING SECTOR FOR MOTORISED CUTTING ME only The Cutting Sector allows the operator to select a suitably slow speed for cutting the sample whilst allowing a fast return stroke Instructions about how to set a Cutting Sector are given in Section 4 9 4 15 HOUSEKEEPING Use a blower to make sure that any debris is regularly cleared away
47. he Brake Lever has three positions that are shown on the colour coded label as shown E version ME version BRAKE MANUAL BRAKE MOTOR MANUAL BRAKE Brake On Brake Off Brake On Motor On Brake Off Brake On Red Green Red Blue Green Red d c d e c d WARNING Care should be taken to make sure that the brake is correctly selected E ME A77510300GB Issue 3 9 HANDWHEEL The Handwheel is used to set the position of the Specimen Head and to manually cut sections The Handwheel is fitted with a fold down handle To fold the handle down pull it outward and fold it down in line with the brake lever a Lift the handle until it clicks into place to use it to section manually b Rotate the Handwheel clockwise to cut sections It is balanced for smooth and easy operation and performs one cutting stroke each time the handle moves through the 1 o clock to 5 o clock sector c The Specimen Head returns to the top of the cycle as the handle moves through 6 o clock to 12 o clock d Note 1 The Handwheel is biased to stop at the top of the cycle The Retract facility allows the specimen to be moved away from the knife edge during the return Stroke to prevent specimen compression The pre set retraction distances are 5um for wax and 40um for resin However these values can be changed by the operator to a value between 5 and 125ym in the Retract Amount Menu section of the User Setup Options described in
48. ht so that the locking knob is at the bottom of the holder and is facing you a Locate the cutout underneath the top section onto the metal slide on top of the base section b so that the locking knob is towards the front of the instrument Gently push the top section onto the slide and position it centrally on the slide To lock the top section in position tighten the locking knob of the top section c by turning it clockwise TO FIT A RALPH GLASS KNIFE The Ralph Knife Holder is suitable for use with 38mm or 25mm high glass knives There are two different Clamp Plates d the 38mm clamp plate is already fitted to the Knife Holder when it is supplied and the 25mm Clamp Plate is o supplied as a spare item e To fit the alternative Clamp Plate remove the two Clamp Screws located on the front the clamp f Remove the Clamp Plate from the Knife Holder and replace it with the other Clamp Plate Make sure the Clamp Plate is oriented as shown To fit a Ralph knife make sure the correct Clamp Plate is fitted onto the Knife Holder Turn the two Clamp Screws f anti clockwise to loosen them Do not remove the Clamp Screws A77510300GB Issue 3 39 Carefully insert the Ralph knife behind the clamp with the cutting edge away from the clamp towards the rear of the instrument e Turn the Clamp Screws clockwise to tighten them until the glass knife is firmly gripped by the clamp TO FIT A TRIANGULAR GLASS KNIFE Turn the
49. hten firmly using the Allen key provided Fit the plastic plug into the hole d Turn the Handwheel until the handle is at the top and apply the brake by moving the Brake Lever to the red position on the label 3 2 3 TO FIT THE KNIFE LOCK LEVER SHAFT The Knife Lock Lever Shaft e must also be fitted to the Finesse when the instrument is first unpacked Press down the Knife Lock Stud f on the Knife Holder Base 22 A77510300GB Issue 3 Fit the Knife Lock Lever Shaft into the hole in the right hand side of the cover so that the cam at the end of the shaft is at the bottom Push the shaft into the hole until approximately 20mm of the shaft is visible Release the Knife Lock Stud Rotate the Knife Lock Lever and make sure that the Knife Lock Stud moves up and down 3 2 4 TO FIT THE DEBRIS TRAY To fit the Debris Tray hold the tray anywhere around its edge and line it up with the front of the instrument under the Knife Holder Push it firmly into position until it clicks into place g To remove the Debris Tray firmly pull it towards YE you h Note 1 Always remove the Debris Tray to lift or move the Finesse WARNING Do not lift the instrument by the debris tray 3 3 ELECTRICAL REQUIREMENTS Make sure that the voltage of the mains supply corresponds Thee with the voltage rating on the rating plate on the back of the zz 2 01 instrument Nominal Ratings 220 240 V C 50 60 Hz 180VA MADE IN UK No
50. inesse so that the small alignment pin c is located towards the rear of the Finesse Secure the Mounting Boss with the screws supplied a Fit the washer b over the Mounting Boss and alignment pin Place the new top tray on top of the Finesse Note 1 The Magnifier requires a separate mains supply socket near to the Finesse 2 Make sure that the voltage of the mains supply corresponds with the voltage rating of the Magnifier WARNING Route the mains cable to the rear of the instrument Make sure it does not come into contact with any moving parts With the magnifying glass facing the front of the Finesse h place the Magnifier onto the Mounting Boss The small pin at the back of the Mounting Boss locates the Magnifier and allows it to be moved to the desired position or moved out of the way 42 A77510300GB Issue 3 The Magnifier can be positioned to suit the individual operator The Magnifier can be moved towards or away from the instrument e To alter the angle of the magnifier loosen the clamping knob f and tilt the Magnifier to the desired angle i WARNING Hold the magnifier in the required position until the clamping knob is tightened To switch on the light press the switch on top of the Magnifier 9 COLD PLATE The Cold Plate provides a convenient cold area to cool specimen blocks Remove the top cover of the Cold Plate a and fill the container with water to the top of the baffles b Replace the
51. ity INDEX 67 APPENDIX A USER SETUP OPTIONS 71 APPENDIX B TRANSPORTATION INSTRUCTIONS 74 APPENDIX C KNIFE AND BLADE PROFILES 78 A77510300GB Issue 3 WELCOME 1 INTRODUCTION Welcome to the Finesse microtome series a family of precision microtomes intended for use by appropriately trained Laboratory Technicians for the routine sectioning of wax or resin embedded tissue specimens that are subsequently prepared and diagnosed by a pathologist Designed and made with care the instrument is safe to use simple to operate and easy to maintain This Operator Guide gives instructions for its correct operation and use of both models of the Finesse E and ME Instructions and information that applies to the ME only are marked as follows ME only 2 SAFETY THIS PARAGRAPH DETAILS IMPORTANT SAFETY INFORMATION PLEASE READ THIS SECTION CAREFULLY Thermo products are designed for convenient and reliable operation and to accepted standards of safety Its use does not entail any hazard if operated in accordance with the instructions given in this manual However incorrect actions by a user may damage the equipment or cause a hazard to health It is important for you to obey the following safety precautions i All users must read and understand the Operator Guide and only operate the unit in accordance with the instructions If the instructions are not followed then the protection provided by the instrument may be impaired
52. lder only To save the angle set the Blade Holder to the required position and turn the Knife Lock Lever to secure it in the desired position Use the Allen key provided to turn the screw in the side of the Blade Holder c anti clockwise to release it Slide the screw until you can feel the locking plunger locate b Tighten the screw To change the angle release the screw and repeat the above procedure FITTING A DISPOSABLE BLADE MICROTOME BLADES ARE EXTREMELY SHARP AND CAN CAUSE SERIOUS INJURY ALWAYS HANDLE MICROTOME BLADES WITH GREAT CARE USE THE DISPENSER WHEN FITTING A NEW BLADE 36 A77510300GB Issue 3 N 0005 PLACE THE BLADE GUARD OVER THE BLADE WHENEVER THE INSTRUMENT IS NOT BEING USED FOR CUTTING Use the handle to swing the knife guard away from the blade clamp Pull the black blade clamp lever towards you to release it Push the end of the blade to be installed part way out of the dispenser and put its end under the plate of the blade clamp Slide the blade until it is located symmetrically in the top stage Push the blade clamp lever to lock the blade in position Take care when feeding _ the blade behind the c Pull the blade clamp blade clamp s lever towards you to unlock Blade Guard TO MOVE THE BLADE LATERALL To move the top stage release the blue blade position clamp lever Move the top stage to the required position and re tighten the blue lever Move blue blade position clamp towards you to
53. lternative mains supplies Maximum supply voltage fluctuations not to exceed 10 of nominal voltage 7 1 3 Fuses Mains plug fuse where applicable 5A 250V Mains input fuses x 2 Transformer fuses x 2 T1 6A 250V T3 15A 250V each secondary Note Fuses should only be replaced by technically competent personnel 7 1 4 Switch convention O 7 1 5 Environment General Temperature operation Power On Power Off Indoor use only 5 C to 40 C Temperature transit storage 25 C to 55 C 70 C for short exposure Humidity Altitude Pollution degree Over voltage category 7 1 6 Thermo part numbers Finesse ME 220 240V ac Finesse ME 110 120V ac Finesse E 220 240V ac Finesse E 110 120V ac 66 pec 80 max for temperatures lt 31 C 50 max for temperatures 31 C to 40 C Non condensing environment up to 2000m 6 500 feet 2 A77500015 A77500016 A77500021 A77500022 wv A77510300GB Issue 3 7 2 ACCESSORIES Orientation Heads Fine Adjust Fixed Head Knife Holders Solid Disposable low profile Disposable high profile Ralph 38mm 25mm Triangular Specimen Holders Vices 60 x 55mm 50 x 45mm 50 x 30mm 25 x 30mm Spacers 50 x 10mm m V 5 10mm diameter Resin Clamp EBH Resin Clamp JB4 Quick Release Clamp General Accessories Cold Plate Ice Tray Cold Point Light Source 230V UK Cold Point Light Source 230V Europe C
54. men being sectioned 45 TOTRIM A SPECIMEN The thickness required while trimming a specimen is set using the Front Panel and Control Unit see paragraphs 4 3 1 and 4 3 2 The vj button can be used to advance a specimen to the knife The Specimen Travel controls and v on the Control Unit can be used to move the Specimen Head towards and away from the knife L moves the Specimen Head away from the knife When held for more than 3 seconds the Specimen Head moves more quickly If it is held until the LED lights the Specimen Head rewinds to the back stop position The green LED will be lit when the Specimen Head is moving to the back stop position Cancel this movement by pressing either or V vj moves the Specimen Head towards the knife When the button is first pressed the Specimen Head slowly moves towards the knife Continue to press v for 3 seconds to move the Specimen Head more quickly A77510300GB Issue 3 49 Release the brake and set to either MANUAL green position or MOTOR blue position ME only Slowly turn the Handwheel clockwise or press RUN if the motor is engaged ME only Each time the handle on the Handwheel moves from the 11 o clock position to the 12 o clock position the Specimen Head will move towards the knife by the amount set MEMORY The Trim Wheel b can also be used to carefully approach the specimen and is able to provide the operator with fine iuro j
55. mon knives are shown at the side of this page Different profiles are shown below C profile Flat backed C profile Round backed C profile Diamond backed D profile 40 parallel sides 311 C 2 DISPOSABLE BLADES RON The profiles of low and high profile disposable blades are shown below 80mm 14mm 76 5mm 86 120mm E E E e e f A77510300GB Issue 3 INDEX Symbols zx Warning symbol 2 N Warning Symbol 2 A Annotated diagram of Finesse 6 APPENDIX A TRANSPORTATION INSTRUCTIONS Product Return Safety Declaration 84 B Brake Lever Settings 9 C CLEANING AND MAINTENANCE 57 Cleaning 57 To Clean the Disposable Blade Holder 57 To Clean the Instrument 57 To Clean the Knife Holder 57 Maintenance 57 CONTENTS 3 CONTROLS 8 Control Unit 12 AUTO 15 MEMORY 14 MODE 17 Continuous cutting Stop Mode 17 Run mode cutting 17 Single cycle cutting 17 RUN 18 SECTOR 16 STOP 18 TRIM SECTION 15 FOOTSWITCH ENABLE 1 15 Rock 14 Specimen Travel Controls 13 Speed Control Wheel 18 To Set Up the Control Unit 12 Trim Wheel 13 Description 8 77510250GB Issue 3 Emergency Stop Button 8 Footswitch 18 Front Panel Controls 11 and 11 CLEAR 11 NEXT 11 RETRACT 12 Backlight 12 Display 11 Standby c 12 Manual Controls 9 Brake 9 Handwheel 10 D Declaration of Conformity 68 Depth 20 DESCRIPTION 6 Overview 6 Dimensions 20 E Earthing gro
56. ntenance is essential for the long term serviceability of precision engineered products such as the Finesse microtomes We recommend that a Service Contract is used to ensure future reliability and consistency of performance This instrument is designed for maximum safety ease of use and reliability It should only be serviced by a Thermo qualified Service Engineer TABLE 1 INSTRUMENT FUNCTION SYMPTOM CAUSE REMEDY Display not lit on the a No Power Supply a Check the mains supply Front Panel b Mains fuse blown b Replace mains fuse Instrument fuse blown c Replace the instrument fuse Note Only a technically competent person should replace fuses The brake is engaged a Make sure the Brake Lever is set to MANUAL or ME only MOTOR ME only Slowly adjust 2 Handwheel will not a turn when turned manually or ME only when RUN is b ME only The Speed b pressed Control Wheel is set to the Speed Control Wheel to its lowest setting increase the speed 3 ME only a The Footswitch cable a Check the Footswitch Footswitch does not has not been properly connection at the rear panel operate correctly connected of the instrument b The Footswitch is disa b Press p to enable the bled Footswitch c Make sure that the Footswitch has not been pressed fully down to the second position A77510300GB Issue 3 59 WAX thick and thin areas parallel to the knife edge
57. old Point Light Source 110V PART NUMBER 77510166 77510164 77510171 A77510272 A77510275 77510168 77510170 77510167 0201 0214 0313 0215 0216 0220 A77510181 77510178 A78110200 77510175 77510174 77510261 77510252 77510262 Note that the Cold Point Light Source includes a twin arm gooseneck light guide Cold Point Light Source spare parts Bulb 8V 20W halogen Fuse 230V version T125mA Fuse 110V version T250mA Mains plug fuse where applicable 3A 250V Illuminated Magnifier 230V UK 1 75 x Illuminated Magnifier 230V Europe 1 75 x Illuminated Magnifier spare parts Fluorescent tube 9W Mains plug fuse where applicable 3A 250V Tool Kit 2 5 mm A F allen key 3 mm A F allen key 6 mm A F allen key Blades See Thermo Catalogue for details Cassettes See Thermo Catalogue for details Knives See Thermo Catalogue for details A77510300GB Issue 3 P13962 P13975 P13741 77510155 77510253 P13934 77510234 1 1 1 67 WARRANTY STATEMENT We are proud of our quality and reliability and of our after sales service We continuously strive to improve our service to our customers Please ask your distributor or representative about Service Contracts which can keep your purchase in peak condition for many years to come Warranty provisions necessarily vary to comply with differences in national and regional legislation and you can find details in your delivery documents or from you
58. on to the next sub menu otherwise press either or to choose again or press CLEAR to return to the Speed Options main menu When NEXT is pressed the display will advance to the SCREEN display used to set the response speed of the screen The pre set value is MEDIUM and the alternatives of HIGH and LOW can be displayed by pressing either or As before when the desired option is displayed press NEXT to select it otherwise press either or to choose again or press CLEAR to return to the Speed Options main Screen When NEXT is pressed the display will advance to the BUTTONS display used to set the response speed of the buttons It also sets the speed of advance of the Specimen Head when vj is pressed see Note 1 below Again the pre set value is MEDIUM and the alternatives of HIGH and LOW can be displayed by pressing either 4 or Select the desired value in the same way as before Note 1 When LOW speed is selected for the buttons there is a single slow advance speed When MEDIUM or HIGH speed is selected there is a two speed advance when vj is pressed the Specimen Head advances to the knife slowly and after a set time advances quickly 78 A77510300GB Issue 3 The second faster advance speed is reached sooner when HIGH speed is selected than when MEDIUM is selected When the desired selections have been made press CLEAR to return to the Spe
59. ount fits into the vice so that the vice can clamp onto either the shaft or the outer diameter of the holder Piel Do not overtighten the clamp onto plastic stubs Note 1 Clamping on the larger outer diameter of the holder will give better stability and rigidity 2 Make sure that the resin mount and specimens are securely clamped 3 4 4 KNIFE HOLDERS INJURY ALWAYS HANDLE MICROTOME BLADES WITH GREAT CARE MESH GLOVES PROVIDE SOME PROTECTION WHEN SHARP OBJECTS ARE HANDLED 1 MICROTOME BLADES ARE EXTREMELY SHARP AND CAN CAUSE SERIOUS AN sione ALL BLADES IN THEIR TRANSPORT BOXES WHEN NOT REQUIRED FOR IMMEDIATE USE INTRODUCTION The Knife Holders for the Finesse microtome are designed to hold a range of blades solid knives disposable blades both low and high profile and glass knives both triangular and Ralph Note 1 Always use a compatible knife Check with your Thermo agent if in doubt Common features of all types of knife and Knife Holder are described in this section The Knife Holder assembly is made of two parts a top and a base The base b is an integral part of the microtome body and supports the top section a 30 A77510300GB Issue 3 The top sections of the assembly can be interchanged with others in the range For clarity the removable top section of the Knife Holder assembly will subsequently be referred to as the Knife Holder AN sos REMOVE THE KNIFE AND APPLY THE HANDWHEEL B
60. ousekeeping 56 To Cut Sections 55 To Mount a Specimen 54 To Set the Cutting Sector for Motorised Cutting 56 To Trim a Specimen 55 WAX EMBEDDED SPECIMENS 47 Housekeeping 53 To Cut Sections 51 Manual Cutting 51 Motorised Cutting ME only 52 To Rock 49 To Set the Cutting Sector for motorised Cutting ME only 52 To Set the Knife Holder for Cutting Specimens 47 Recommended Knife Angles 48 To Set the Knife Holder to the Required Angle 48 To Trim a Specimen 48 To Use the Memory 50 P PRODUCT RETURN SAFETY DECLARATION 84 S SPECIFICATION AND ACCESSORIES 65 Accessories 66 General Accessories 66 Knife Holders 66 Orientation Heads 66 77510250GB Issue 3 Specimen Holders 66 Width 20 Specification 65 Wiring Convention 24 Electrical 65 Environment 65 Fuses 65 Physical 65 Switch Convention 65 Thermo part number 65 T TRANSPORTATION INSTRUCTIONS 81 Introduction 81 To Repackage the Finesse 81 TROUBLE SHOOTING 58 General 58 Table 1 Instrument Function 58 Table 2 Problems when Sectioning Wax Embedded Specimens 59 Table 3 Problems when Sectioning Resin Embedded Specimens 61 Table 4 Warning Screens 62 U USER SETUP OPTIONS 69 Flow Charts of User Setup Selections 71 Auto Load Options Menu 79 Display Options Menu 74 Language Select Menu 71 Material Select Menu 72 Retract Select Menu 73 Speed Options Menu 77 Introduction 69 To enter User Setup 69 To exit User Setup 69 Overall Flow Char
61. peed Knob g sets the speed within the Cutting Sector At low cutting speeds the return speed is automatically faster than the cutting speed to save time To clear the Cutting Sector press and hold SECTOR until both LEDs on the button clear 1 6 A77510300GB Issue 3 2 5 10 MODE VE only The MODE button is used to step through the available cutting modes Single Cycle Mode 1 Run Mode continuous cutting RUN and Stop Mode continuous cutting STOP 2 5 11 Single cycle cutting ME only Jie The green LED marked 7 lights when this option is selected g t The RUN button a on the Control Unit starts a cut cycle when it is pressed once The Specimen Head completes one cycle and automatically stops at the top of its travel If RUN is held down cutting is continuous until RUN is released The specimen head will then stop at the top of its travel THE SPECIMEN WILL CONTINUE TO MOVE UNTIL IT REACHES THE TOP OF ITS TRAVEL 2 5 12 Run mode cutting ME only The green LED marked RUN lights when this option is selected Cutting is continuous as long as RUN is pressed When RUN is released the motor stops immediately with the specimen head in any position 2 5 13 Continuous cutting Stop mode ME only The green LED marked STOP lights when this option is selected Continuous cutting is started when RUN is pressed once If STOP is
62. pressed the specimen will stop immediately with the specimen head in any position If RUN is pressed again while the instrument is cutting the specimen moves to the top of its travel and then stops A77510300GB Issue 3 17 Ave SPECIMEN CONTINUES TO MOVE UNTIL IT REACHES THE TOP OF ITS TRAVEL 2 5 14 SPEED CONTROL WHEEL ME only This is used to set the speed of motorised cutting The display shows the speed selected when an adjustment is made The display will then return to the previous screen Note Speeds will automatically differ between TRIM and SECTION modes and will be displayed as 1 99 To display the speed at all times see the Display Options Menu pec of the User Setup Options described in Appendix A Section ain A 3 4 EM C 2 5 15 RUN ME only RUN starts a cutting cycle according to the mode set and lights the green LED Refer to MODE section paragraphs 3 5 10 to 3 5 13 Note 1 The RUN button on the Control Panel will not allow cutting if the footswitch has been enabled A warning message will be shown on the display 2 The Brake Lever should be set to the MOTOR position blue for motorised cutting 2 5 16 STOP ME only STOP stops any active cutting cycle immediately 2 6 FOOTSWITCH ME only Note 1 The Footswitch must be fully depressed at least once after switching on the mains supply before it can be enabled 2
63. ption is given about each individual menu KEY PRESSES TO ENTER USER SETUP PRESS 8 PRESS MEMORY RELEASE 8 RELEASE MEMORY Paragraph A 3 1 LANGUAGE SELECT MENU NEXT CLEAR MATERIAL SELECT MENU NEXT Paragraph A 3 2 CLEAR RETRACT AMOUNT MENU Paragraph A 3 3 NEXT DISPLAY OPTIONS MENU Paragraph A 3 4 NEXT CLEAR SPEED OPTIONS MENU NEXT 3 Paragraph A 3 5 CLEAR AUTO LOAD OPTIONS MENU NEXT Paragraph A 3 6 A77510300GB Issue 3 71 A 3 FLOW CHARTS OF USER SETUP SELECTIONS The following flow charts show the detail of the User Setup selections Note 1 Underlined selections signify the pre set option or value A 3 1 LANGUAGE SELECT MENU The initial language screen is English UK If this is the language required by the operator press NEXT otherwise press until the desired language is shown on the display Press NEXT to select it When NEXT is pressed the display will advance to show the Material Select Option screen that is described fully in paragraph A 3 2 Flow chart A 3 1 shows the sequence of the Language Select menu Note 1 To exit User Setup at any time press and release After a short pause the Finesse will exit to the TRIM or SECTION screens Flow Chart A 3 1 LANGUAGE SELECT MENU ENTRY K
64. r adjustments Each click on the Trim Wheel corresponds to 8um TRM SECTION If the Trim wheel is used only advance the Specimen Head when the WARNING specimen is above the knife Note 1 In TRIM mode the retraction facility is not automatically selected To turn it on press RETRACT 2 Do not advance with the Trim Wheel if the specimen is retracted amber LED is lit Alternatively the rock technique can be used This is described fully in Section 4 6 Continue trimming until the desired face of the specimen is presented to the knife Apply the handwheel brake when trimming is completed 46 TO ROCK The rock facility is an alternative method that can be used to trim the specimen without turning the Handwheel completely When the Handwheel is turned for a small amount the Specimen Head is advanced by a set distance Only use the rock facility to advance the Specimen Head when the WARNING specimen is above the knife 50 A77510300GB Issue 3 Note 1 The ROCK and RETRACT facilities cannot be used at the same time 2 When WAX is selected as the chosen medium the status of ROCK and RETRACT can be different for TRIM and SECTION To enable the rock facility the rock position must be saved Turn the Handwheel to move the specimen to the start or top of the cutting sector a Press ROCK on the Control Uni
65. r dealer or representative Please note that your warranty may be invalidated if the instrument is modified in any way accessories and reagents are used that are not approved by Thermo or the instrument is not operated or maintained in accordance with the instructions in this Operator Guide 68 A77510300GB Issue 3 Declaration of Conformity This Declaration of Conformity is only valid when the instrument is used in accordance with this Operator Manual Manufacturer s Name Thermo Shandon Limited Trading as Thermo Fisher Scientific Manufacturer s Address Tudor Road Manor Park Runcorn Cheshire WA7 1TA ENGLAND Product Description Electronic Microtome Product Designation Finesse E Finesse ME Part numbers A77500015 A77500016 A77500021 A77500022 including accessories supplied as standard and the following accessories Vices 77510167 0201 0214 0215 0216 0220 0313 77510178 A77510181 A78110200 Knife Holders 77510168 77510170 77510171 77510173 A77510263 A77510265 A77510272 A77510275 Year of Marking CE 1998 This product conforms with the essential requirements of the following directives In Vitro Diagnostics Directive 98 79 EC Low Voltage Directive 73 23 EEC as amended by 93 68 EEC This product complies with the following International Standards EMC EN61326 2 6 EN61000 3 2 EN61000 3 3 Safety IEC 1010 1 CAN CSA C22 2 No 1010 1 92 UL Std No 3101
66. ract Amount Menu Note 1 To exit User Setup at any time press and release After a short pause the Finesse will exit to the TRIM or SECTION screens 74 A77510300GB Issue 3 Flow Chart A 3 3 RETRACT AMOUNT MENU FROM MATERIAL SELECT MENU Paragraph A 3 2 NEXT CLEAR AUTORETRACT AMOUNT 5p NEXT RESIN 5um 10um 15um 20um 25um 30um 35um 40um 60um 80um 100um Y 125um CLEAR TO DISPLAY OPTIONS MENU Paragraph A 3 4 A 3 4 DISPLAY OPTIONS MENU This option allows the operator to specify the information to be displayed during normal operation The initial screen displayed is the Display Options Menu Press to enter the different sub menus To advance to the Speed Options Menu paragraph A 3 5 without changing any display options press NEXT or to return to the Retract Amount Menu paragraph A 3 3 press CLEAR If was pressed the first option specifies whether the header should be shown It will be displayed during normal running unless changed Press or to switch between the options of SHOW or OMIT When the desired option is displayed press NEXT to select it otherwise press either or to choose again or press CLEAR to return to the Display Options main screen A77510300GB Issue 3 75 When NEXT is pressed the display will advance to the next option The different op
67. re set for wax LOSE pre set for resin A77510300GB Issue 3 81 APPENDIX B TRANSPORTATION INSTRUCTIONS 8 1 INTRODUCTION If you ever need to transport the Finesse follow these packaging instructions 8 2 TO REPACKAGE THE FINESSE Remove the Knife Holder a and Specimen Holder b from the Finesse Move the Orientation Head as far back as it will go c Note If the Finesse is to be serviced or returned to Thermo it must be decontaminated and the Product Return Safety Declaration Certificate see page B 5 signed Switch off the Finesse and disconnect the mains cable from the mains supply Remove the mains cable from the rear panel of the Finesse d and keep safely j f Disconnect the Footswitch if fitted f e DO NOT REMOVE THE CONTROL UNIT CONNECTOR e FROM THE REAR PANEL OF THE FINESSE d To remove the Debris Tray pull it firmly towards the front of the instrument a Place it in its packing box To remove the Knife Lock Lever shaft b push the Knife Lock Stud f down and pull the Knife Lock Lever Shaft out To remove the Handwheel turn the Handwheel until a the Handle is at the bottom c and apply the Brake Remove the plastic plug from the centre of the Handwheel Remove the screw and washer d using the Allen key provided and place these in a small bag Carefully withdraw 82 A77510300GB Issue 3 the Handwheel from the
68. t changing any options press NEXT or to return to the Speed Options Menu paragraph A 3 5 press CLEAR If was pressed the first screen displayed is the MEMORY OFFSET sub menu used to set the offset from the memory position to allow for unloading the specimens The pre set setting is 5mm which means that the specimen head will withdraw 5mm from the set memory position to allow the specimen to be quickly and safely unloaded Press or to switch between the different values shown below 0 1 2 8 4 5 10 15 20 25 30mm When the desired value is displayed press NEXT to select it and move on to the ROCK POSITION sub menu otherwise press either or to choose again or press CLEAR to return to the Auto Load Options main screen When NEXT is pressed the display will advance to the HOCK POSITION display The pre set options of this menu depend on whether WAX OR RESIN were previously selected If WAX was selected the pre set option is to KEEP the rock position If this is suitable press NEXT otherwise press either or to display the alternative option LOSE If RESIN was selected the pre set option is to LOSE the rock position Again if this is suitable press NEXT otherwise press either or to display the other option KEEP Note 1 The factory settings of the HOCK POSITION option are most useful when blocks of the same size are normally
69. t maintenance procedures are essential for consistent performance It is recommended that a Maintenance Contract is taken out with your supplier The instrument must be serviced annually by a Thermo trained engineer in accordance with the instructions contained in the Finesse Service Manual 77510162 Any problems and queries should be referred to your supplier A77510300GB Issue 3 5 DESCRIPTION 1 1 FINESSE OVERVIEW The Finesse is a precision engineered bench top rotary microtome with electronic advance manual or motorised cutting and a separate control unit that can be sited either on the microtome or on the bench beside it The Finesse has been designed for general laboratory use It can be used to section wax plastic and resin embedded specimens The different components of the Finesse are shown in the diagram 20 ro Q909 0 9 Special design features of the Finesse include vii viii xi xii xiii xiv Control Unit Knife Holder Debris Tray Orientation Head Front Panel Handwheel Emergency Stop Specimen Storage Tray section cutting down to 0 5um thickness fixed knife position with sectioning performance maintained over full specimen travel handwheel braking in any position optional specimen retraction high speed advance and rewind control fine advance and rewind control automatic start positioning for specimen trimming section counting distance travelled display backlight to aid accurate sp
70. t of User Setup Selections 70 Ww WARRANTY STATEMENT 67 Weight 20 WELCOME 4 Introduction 4 Safety 4 77510250GB Issue 3 89
71. t to save the start of the rocking sector b The green LED lights when the facility is selected e Move the Handwheel so that the specimen passes across the knife and then back up again c When the specimen is above the set rock position the amber LED on the button d lights The Specimen Head advances by the set distance c d Note 1 f a Cutting Sector has previously been set the Finesse will use the existing top position when ROCK is pressed e To cancel the rock facility press ROCK again 4 7 TO USE THE MEMORY To help reposition similar sized specimens press MEMORY on the Control Unit when the Specimen Head has advanced to the required position to start to trim The green LED is lit to indicate that a position is saved If AUTO is then pressed the Specimen Head rewinds a set distance back from the knife So that the specimen cassette can be removed The distance can be set in the Auto Load Options Menu section of User Setup described in Appendix A section A 3 6 A77510300GB Issue 3 51 When the next specimen has been loaded press AUTO again to return the Specimen Head to the memory position Note 1 If AUTO is pressed when a memory position has not been saved the Specimen Head rewinds back to the back stop position If the Specimen Head is at the memory position press MEMORY once to clear the memory position If the Specimen Head is not at the memory position press MEMORY
72. te 7 The symbol on the rating plate indicates that the instrument operates on an alternating current Supply a c s Make sure that the 0 power switch at the rear of the instrument is switched off O side of the switch pushed inward a A77510300GB Issue 3 23 Instruments are supplied with power cords with moulded plugs suitable for the UK France and Germany or North America If another plug is required it is necessary for a technically competent person to remove the moulded plug and fit a suitably rated fused plug using the wiring convention shown below Brown wire Live L or L2 terminal Blue wire Neutral N or L1 terminal Green yellow wire Earth terminal E ground or L Insert the appropriate mains cable into the mains connector on the rear panel of the instrument and clip the cable restraint over the mains connector e Connect the mains supply cable to the local power supply outlet Note Connectors c and d are for use by the c Service Engineer only THE FINESSE MICROTOME MUST BE PROTECTIVELY EARTHED MAKE SURE THAT THE INSTRUMENT IS PLUGGED INTO A PROPERLY EARTHED MAINS SUPPLY AN MUST BE POSSIBLE TO INTERRUPT THE MAINS SUPPLY AT SOURCE BY REMOVING THE PLUG FROM THE MAINS SUPPLY SOCKET IF THE CONTROL UNIT HAS BEEN DISCONNECTED FROM THE MAIN INSTRUMENT FOR ANY REASON SWITCH OFF THE FINESSE BEFORE THE CONTR
73. the Clamp Screw located at the side of the Knife Holder to loosen it a Very carefully insert the triangular knife so that it locates against the endstop b Turn the Clamp Screws clockwise to tighten them c until the glass knife is firmly gripped by the clamp Note The knife Knife Holder and Knife Holder Base must all be extremely secure and rigid before starting to section Poor clamping may result in chatter or uneven sectioning 3 5 SECTIONING PROCEDURE WAX AAN sos APPLY THE BRAKE BEFORE ADJUSTING THE SPECIMEN CLAMP OR KNIFE HOLDER MICROTOME BLADES ARE EXTREMELY SHARP AND CAN CAUSE SERIOUS INJURY ALWAYS HANDLE MICROTOME BLADES WITH GREAT CARE MESH GLOVES PROVIDE SOME PROTECTION WHEN SHARP OBJECTS ARE HANDLED STORE ALL BLADES IN THEIR TRANSPORT BOXES WHEN NOT REQUIRED FOR IMMEDIATE USE 40 A77510300GB Issue 3 Use a scraper to remove excess wax from the sides ends and back of the cassette The cassette must be able to mount squarely on the Specimen Clamp Turn the handwheel until the handle is at the top at the 12 o clock position engage the brake red position and test to make sure the brake is on Check that the Specimen Clamp is clean and free of wax deposits Check that the cassette sits squarely and securely in the clamp before you begin to trim the specimen 3 6 SECTIONING PROCEDURE RESIN ALWAYS APPLY THE BRAKE BEFORE ADJUSTING THE SPECIMEN CLAMP OR KNIFE HOLDER INJURY ALWAYS HANDLE MICROT
74. the lower holes c to secure it 3 4 6 DISPOSABLE BLADES INJURY ALWAYS HANDLE MICROTOME BLADES WITH GREAT CARE MESH GLOVES PROVIDE SOME PROTECTION WHEN SHARP OBJECTS ARE HANDLED MICROTOME BLADES ARE EXTREMELY SHARP AND CAN CAUSE SERIOUS EXCESSIVE CLAMPING OF THE BLADE MAY DISTORT THE CLAMPING PARTS AND THE BLADE DISPOSABLE BLADE HOLDER Part numbers A77510272 low profile disposable blade holder complete A77510275 high profile disposable blade holder complete FITTING THE BLADE HOLDER The bottom stage enables the blade angle to be adjusted and the top stage allows lateral movement of the clamped blade The blade guard is attached to the top stage Aw LOCK THE HANDWHEEL TO PREVENT IT MOVING WHEN WORKING NEAR THE BLADE HOLDER To fit the Blade Holder move the Knife Lock Lever on the right hand side of the instrument b until the T stud locking pin on the Blade Holder Base a is at its highest point Locate the T shaped cutout section on the bottom of the Blade Holder onto the T stud a 34 A77510300GB Issue 3 Slide the Blade Holder onto the base making sure that the T stud slides along the cutout Note Top Stage 1 The Blade Holder should not be too loose when fitted onto blade support the base If it feels loose remove the Blade Holder and adjust the T stud a by turning it clockwise to lower the stud Bottom 2 If the Blade Holder will not slide on at all or is very tight Stage
75. tings e The brightness of the light increases from setting I through to setting III Adjust the arms to the desired position f To switch the light unit off turn the Brightness Knob to the O setting e 44 A77510300GB Issue 3 OPERATION 4 1 INTRODUCTION Nis INSTRUMENT USES SHARP KNIVES MAKE SURE YOU UNDERSTAND THE SAFE AND CORRECT METHODS OF USING AND HANDLING THEM MESH GLOVES PROVIDE SOME PROTECTION WHEN SHARP OBJECTS ARE HANDLED STORE BLADES IN THEIR TRANSPORT BOXES WHEN NOT REQUIRED FOR N N N ALWAYS APPLY THE BRAKE BEFORE ADJUSTING THE SPECIMEN CLAMP OR VICE OR HANDLING THE KNIFE AWAYS WEAR SAFETY SPECTACLES WHEN USING GLASS KNIVES All motors are automatically disabled if the red Emergency Stop button 5 a a has been pressed To disengage the Emergency Stop turn the ec red button clockwise until it unlocks and pops out Then follow the i instructions shown on the display 111 Oo EH It is recommended that the brake is applied and the instrument is kept in Standby mode when it is not in use To enter the Standby mode press the 6 button Note o 1 The red LED lights when the instrument is in STANDBY mode 5 To restart the instrument press the button again A77510300GB Issue 3 45 4 2 NORMAL OPERATION This section deals with the normal operation of the Finesse microtome Note 1 Many features and displays of the Finesse can be customised using the User
76. tions are HEADER COUNT DISTANCE DUAL DISPLAY SPEED TRIM TRIM or SECTION displayed 10u at the top of the screen eg Total number of sections taken the number of times the specimen head has advanced Total distance the specimen head has travelled R is displayed if the specimen head has retracted for the return stroke Both COUNT and DISTANCE displayed at the same time The speed of the cutting motor ME Only Use the and NEXT buttons to select the desired options in each of the sub menus in turn When the desired selections have been made press CLEAR to return to the Display Options main screen and then press NEXT to go on to the Speed Options Menu display paragraph A 3 5 Flow chart A 3 4 shows the sequence of the Display Options Menu Note 1 The Finesse will display all information except speed unless the options are changed 2 If CLEAR is pressed and held down when the initial Display Options screen is displayed all five sub menus will revert to their factory set values 3 If CLEAR is pressed and held down in any of the sub menus the individual option will always revert to the factory set option 4 To exit User Setup at any time press and release S After a short pause the Finesse will exit to the TRIM or SECTION screens 76 A77510300GB Issue 3 Flow Chart A 3 4 DISPLAY OPTIONS MENU FROM RETRACT AM
77. top cover and freeze until completely frozen To fit the Cold Plate onto the Finesse place it on the rear section of the top tray c To clean the Cold Plate remove the top plate heatsink and baffles d Wash in warm soapy water ICE TRAY The Ice Tray is an alternative to the Cold Plate Fill the container with water to the top of the baffles e Freeze until completely frozen Place the frozen Ice Tray onto the top tray of the Finesse f A77510300GB Issue 3 To clean the Ice Tray remove the top plate heatsink and baffles Wash in warm soapy water COLD POINT LIGHT SOURCE The Cold Point Light Source is a separate free standing c accessory that can be adjusted by the operator to give optimum illumination of the specimen using a light that will not affect the temperature of the specimens Note 1 The Light Source requires a separate mains supply socket near to the Finesse 2 Make sure that the voltage of the mains supply corresponds with the voltage rating of the Light Source Turn the Locking Knob towards the 0 symbol a Firmly insert the flexible light arms into the connector on the front of the base unit b with the small metal pin on the light arm connector at the top c Secure the flexible arms by turning the Locking Knob towards the 0 symbol d Switch on the mains supply to the base unit and switch on by turning the Brightness Knob to the I Il or Ill set
78. unding requirements 5 24 Emergency Stop Button 8 F Footswitch 15 18 24 H Height 20 INSTALLATION AND SETTING UP 20 Accessories 41 Cold Plate 42 Cold Point Light Source 43 Ice Tray 42 Magnifier Europe Only 41 Electrical Requirements 23 Introduction 20 Sectioning Procedure Resin 40 87 Sectioning Procedure Wax 39 To Install the Instrument 20 To Fit the Debris Tray 23 To Fit the Handwheel 21 To Fit the Knife Lock Lever Shaft 22 To Unpack 20 To Set Up the Instrument 25 Disposable Blades 34 Disposable Blade Holder 34 Disposable Blade Holder with Wide Clamp Plate 35 Glass Knives 37 Glass Knife Holders 37 To Fit a Ralph Glass Knife 38 To Fit a Triangular Glass Knife 39 To Fit the Glass Knife Holder Top Sections 37 Knife Holders 30 Introduction 30 To Fit the Knife Holder to the Base 31 To Save the Knife Holder Position 32 To Set the Required Angle 32 Solid Knives 33 Solid Knife Holder 33 Specimen Clamping 28 Quick Release Cassette Clamp 29 Vices 29 Specimen Orientation 26 Fine Adjust Orientation Head 26 Fixed Head 27 Orientation Heads 26 To Switch On and Off 25 To Switch Off 26 To Switch On 25 K KNIFE AND BLADE PROFILES 85 Disposable Blades 85 Introduction 85 Solid Knives 85 88 0 OPERATION 44 Introduction 44 Normal Operation 44 Operating Displays 45 COMBINED Display 47 COUNT Display 46 DISTANCE Display 47 SECTION Display 46 TRIM Display 45 RESIN EMBEDDED SPECIMENS 53 H
79. used If blocks of different sizes are regularly used it is helpful to change the pre set selections to avoid the possibility of the specimen inadvertently advancing into and damaging the knife 2 The pre set option for wax is to save the rock position The pre set selection for resin is to discard the rock position 3 If CLEAR is pressed and held down when the SPEED OPTIONS screen is displayed all three sub menus will revert to their factory set pre set values 80 A77510300GB Issue 3 4 To exit User Setup at any time press and release 5 After a short pause the Finesse will exit to the TRIM or SECTION screens Press NEXT to select the desired option otherwise press either or to choose again or press CLEAR to return to the Auto Load Options Menu paragraph A 3 6 When the desired selections have been made press CLEAR to return to the Auto Load Options main menu and then press NEXT to go on to the Language Select Menu paragraph A 3 1 or to exit User Setup Flow chart A 3 6 shows the sequence of the Auto Load Options Menu Flow Chart A 3 6 AUTO LOAD OPTIONS MENU FROM SPEED OPTIONS MENU Paragraph A 3 5 NEXT CLEAR AUTO LOAD OPTIONS to set NEXT CLEAR MEMORY OFFSET 5mm NEXT CLEAR Keep rock position NEXT TO LANGUAGE SELECT MENU Paragraph A 3 1 Note KEEP p
80. ustment Lock on the right as shown e Use the Allen key and the three remaining long screws and washers to secure the Orientation Head to the Mounting Plate f 26 A77510300GB Issue 3 WARNING Tighten firmly using only the tools provided Fit the 3 plastic plugs into the front three holes to cover the screwheads Jh To attach the vices and specimen clamps to the Fine Adjust Orientation Head clamp the Orientation Head by moving the Adjustment Lock until it is vertical in either direction g Screw the vice or clamp onto the threaded stud protruding from the centre of the Orientation Head h until it is secure AN 0 05 APPLY THE HANDWHEEL BRAKE BEFORE ADJUSTING THE SPECIMEN CLAMP OR VICE To adjust the orientation of the Specimen Clamp turn the Adjustment Lock on the right hand side of the Orientation Head to point towards the front of the instrument i so that the specimen holder has free movement The ends of the two Orientation Head Adjustment Levers can be rotated to position the Specimen Head accurately j The specimen clamp or vice can also be rotated for 360 To secure the Orientation Head when it is in the required orientation tighten the Adjustment Lock until it is vertical in either direction k Make sure that the Specimen Clamp is secure and does not move as this will affect the success of sectioning FIXED HEAD AN Revove THE KNIFE AND KNIFE HOLDER FROM THE FINESSE BEFORE A FIXED HEAD
81. wards you a To replace the Control Unit insert the bottom of the Control Unit into its holder and lower the back of the Control Unit The Control Unit has flip down feet that can be lowered or left up to suit individual preferences The operation of the individual buttons Trim Wheel b and Speed Control Wheel c are described in the following section 2 5 2 SPECIMEN TRAVEL CONTROLS c ME ae ISECTOR mope o AD a 8 g R 8 8 2 moves the Specimen Head away from the knife When held for more than 3 seconds the Specimen Head moves more quickly see Note 1 below If it is held until the LED lights the Specimen Head rewinds to the back stop position Cancel this movement by pressing either 4 or v v moves the Specimen Head towards the knife Note To change the response speed of the buttons and the speed of advance of v refer to the Buttons Display in the Speed Options Menu of the User Setup Appendix A section A 3 5 2 5 3 TRIM WHEEL The Trim Wheel provides fine adjustment of the Specimen Head position The distance of the Trim Wheel adjustment depends on whether wax pre set value of 8um or resin trim distance set is selected see the Material Select Menu option in the User Setup Options Appendix A section A 3 2 A77510300GB Issue 3 TRM SECTION
82. y retracted o RETRACT If the Retraction option has been selected an R on the distance travelled display shows that the Finesse has retracted the Specimen 2 4 6 BACKLIGHT izj switches the knife holder backlight on and off 1 2 4 7 STANDBY KEY 6 switches the instrument into standby mode The red LED is lit when Standby is selected All motorised cuts in progress will be stopped and all motors LEDs the by display and the knife holder backlight will be turned off if 5 is pressed The display shows the Finesse logo as the instrument saves all the current settings before entering Standby mode If is pressed again the instrument will restart using the previous settings AAN js APPLY THE BRAKE BEFORE SWITCHING THE INSTRUMENT INTO STANDBY MODE Note 1 Switch the instrument into Standby mode when not in use 2 CONTROL UNIT 2 5 1 To set up the Control Unit The Control Unit is connected to the instrument by electrical cable The Control Unit unclips from the body of the instrument for ease of use 12 A77510300GB Issue 3 It is not necessary to remove the Control WARNING Unit cable from the rear of the Finesse However if the Control Unit has been disconnected from the main instrument for any reason make sure the Finesse is switched off before the Control Unit is reconnected otherwise damage will occur To remove the Control Unit from its holder pull the back of the Control Unit upwards to

Finesse E+ and ME+ Operator Guide

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