QBC® Capillary Centrifuge Operator`s/Service Manual
Contents
1. Under centrifugation blood cells in the buffy coat WHITE CELLS layer separate according to their densities forming CXMPHOUYTES visibly discrete bands as shown in Figure 1 2 Platelets MONOS YTES constitute the topmost band lymphocytes and monocytes the center band while granulocytes the heaviest band form immediately above the packed RED CELLS red cells AROUND FLOAT GRANULOCYTES STOPPER Figure 1 2 Cell layers in Centrifuged QBC Hematology Tube 1 2 1 QBC Centrifugal Hematology Tests continued Coated reagents in the QBC blood tube enhance the visual separation between cell layers and provide differential staining so that tubes can be read by non automatic and automatic QBC analyzers 1 2 2 QBC Malaria Test The centrifugal test for detecting malaria parasites is based on a high precision glass capillary tube containing an equally precise plastic float When the blood filled tube is centrifuged at high speed for five minutes the float is buoyed by the packed red cells automatically positioning the float within the buffy coat layer between the packed red cells and the plasma During centrifugation blood cells in the buffy coat separate according to their densities forming visibly discrete bands Figure 1 3 Platelets constitute the topmost layer lymphocytes and monocytes the middle layer and granulocytes being the heaviest of the buffy coat cells concentrate immediately above the packed red cells Reagents coat2. ANA vm Em mo ae BEEN e e WEE wl m Diagnostics QBC Capillary Centrifuge Operator s Service Manual ii A Ze E gn 5 WES SEE wa Diagnostics Innovative Solutions for a Healthier World QBC Capillary Centrifuge Operator s Service Manual QBC QBC Diagnostics ParaLens and ParaLens Advance are trademarks of QBC Diagnostics Inc Copyright 2006 by QBC Diagnostics Inc and Vacutainer is a trademark of Becton Dickinson amp Company 4740 020 004 Rev G Table of Contents Section 1 INTRODUCTION 1 aire ua ecd Eddie rue kot iiaa danaa ntis beu brune dn 1 TEINIENDEB DSE teretes eebe eet 1 1 2 SUMMARY AND EXPLANATION A 1 1 2 1 QBC Centrifugal Hematology Tests cccccececcceeeeesenceeeeeseeceeeneeseneee snes n NEEE OSSR 1 t22 GOBC Malatia EE 2 1 9 PRINCIPLES OF THE PROGCEDUBRES 2 2 inira ee cete tercie Tada sadaa terc ected ceded 2 1 3 1 QBC Centrifugal Hematology Tests 2 UcAezsq uriEura e aaea 3 1 4 WARNINGS ANB PRECAUTIONS 2 22 15r Ec rp eerie aee ug eut iu coe o reri EXER ERE iaioa arana aidan aet 3 1 5 SYMBOL DEFINITIONS 5 ses ceva naruto ro es e hu LR teed dae Ric p Rr Ra dee Dea ra rai rss ere epu dan E yel 3 Section 2 SYSTEM DESCRIPTION cis vices css i iex oit phe na cix se cad ean ERE EE RR DR X RR EE Eae ERRAT aL an de KR Saanika St 4 21 COMPONENTS NEY 4 22
3. On Or Flashing Latch Solenoid Wait for Problem Rotor to Stop Unplug Let Yellow Light Bleed Out Turn On Lid May Now Pop Open 24 Lid Latched Check Yellow Light Flashing Steady Turn Off Let Yellow Light Bleed Out Turn On Lid Will Pop Open Close Firmly and Try Again Noisy Vibrates Remove and Reseat Rotor Cover Don t Overtighten Check Tube Balance Note Momentary vibration rumble is normal during acceleration and deceleration 9 5 TROUBLESHOOTING continued The combinations of On Off and Flashing status of the POWER and SPEED LED s shown below provide visual indicators of the current operating condition LOL UE Flashing Ready to spin lid latched Press ON button Power on no rotor motion lid not latched Load or unload tubes On Flashin Power on lid latched Rotor is usually accelerating 9 rotor under speed to design speed Power on lid latched e Power on lid latched 9 6 PARTS LIST The following spare parts and accessories for the QBC Capillary Centrifuge are available from your QBC System distributor or through the nearest QBC Diagnostics Inc office Part Description Catalog No Rotor Head 421289 Head Nut 421290 Rotor Cover 421291 Head Nut Wrench 421292 Rotor Removal Wire 4271 020 006 Line Cords Power Pack United States 421634 Europe 421551 United Kingdom 421554 Power Pack 421763 Operator s Manual 422928 25 WARRANTY QBC Cap
4. 3 TEMPERATURE REQUIREMENTS For QBC Hematology Tests Optimum results are obtained when the laboratory temperature is maintained between 20 to 32 C 68 to 90 F To avoid excessive heating of blood tubes during spinning locate the centrifuge away from direct sunlight For QBC Malaria Test Optimum results are obtained when test procedures are performed at temperatures between 16 to 37 C 60 to 99 F IMPORTANT For room temperature specifications Consult the package insert of the disposables in use in your laboratory 6 4 CENTRIFUGATION INSTRUCTIONS CAUTION DO NOT USE THIS CENTRIFUGE FOR SPINNING MICROHEMATOCRIT OR OTHER CAPILLARY TUBES THE ROTOR AND COVER ARE DESIGNED TO ACCOMMODATE ONLY QBC TUBES OTHER TYPES OF CAPILLARIES MAY BREAK CAUSING BLOOD SPILLS AND LOSS OF SPECIMEN 6 4 1 Affixing Identification Labels QBC Malaria Test Only Before Placing QBC Malaria Test tubes on the centrifuge rotor a correctly marked patient identification label should be affixed on each tube between the two white lines scribed near one end of the tube Figure 6 1 Figure 6 1 Placement of ID Label on QBC Malaria Test 6 4 2 Loading and Balancing Rotor Position QBC specimen tubes in the holder slots of the rotor with the stopper against the outer rim of the rotor Arrange the tubes in a balanced array i e 2 tube opposite 1 tube 4 opposite 3 etc When centrifuging an odd number of tubes balance the roto
5. For technical assistance with QBC hematology tests contact 1 866 265 1486 toll free 1 814 692 7661 QBC Diagnostics Inc 168 Bradford Drive Port Matilda PA 16870 U S A 1 3 2 QBC Malaria Test The QBC Capillary Centrifuge is designed to spin up to 20 QBC Malaria Test tubes at the force time requirements needed to cause the infected cells to concentrate in a narrow area of the tube for easy identification The QBC Malaria Test is a 75 mm glass capillary tube internally coated with potassium oxalate acridine orange stain and anticoagulants Expansion of the separated cell layers is achieved with a plastic float which is inserted into the tube prior to centrifugation Rotor speed and timing of the centrifuge are computer controlled for consistent performance For malaria detection in the centrifuged sample the tube is examined under a fluorescence microscope or the ParaLens Advance in the region between the light red blood cells and granulocytes and lymphocytes monocytes where the parasites are most abundant For technical assistance with the QBC Malaria Test contact 1 866 265 1486 toll free 1 814 692 7661 QBC Diagnostics Inc 168 Bradford Drive Port Matilda PA 16870 U S A 1 4 WARNINGS AND PRECAUTIONS Always observe good laboratory procedures when handling patient specimens WARNING BLOOD SPECIMENS USED FOR THESE PROCEDURES MAY CONTAIN THE HEPATITIS B AND C VIRUS HUMAN IMMUNODEFICIENCY VIRUS HIV OR OTHER INFECTIO
6. HANDLING PRECAUTIONS Exercise good laboratory procedures when collecting and handling patient specimens WARNING BLOOD SPECIMENS USED FOR THESE PROCEDURES MAY CONTAIN THE HEPATITIS B AND C VIRUS HUMAN IMMUNODEFICIENCY VIRUS HIV OR OTHER INFECTIOUS DISEASES HANDLE BLOOD AS A POTENTIAL BIOHAZARD CAPABLE OF TRANSMITTING INFECTION ALWAYS WEAR GLOVES AND OTHER APPROPRIATE BLOOD BARRIER PROTECTION WHEN COLLECTING AND PREPARING BLOOD e Be sure to clean the skin area with an antiseptic agent dry the area with a sterile wipe Puncture only with a sterile lancet Always use sterile needles and collection tubes 5 3 SPECIMENS FOR QBC HEMATOLOGY TESTS Venous blood for QBC hematology test analysis can be collected in Vacutainer brand tubes containing EDTA anticoagulant QBC tubes for venous blood are filled by means of a semi automatic pipetter supplied with the QBC System QBC capillary blood tubes are pre coated with anticoagulants and are filled with blood directly from a finger puncture After filling with blood the tubes are sealed with a rubber stopper or plastic closure The float in QBC tubes expands the buffy coat cell layers during centrifugation as previously described Stability For stability specifications consult the package insert of the disposables in use in your laboratory 5 4 SPECIMENS FOR QBC MALARIA TEST Collect capillary blood from the finger or foot directly into the QBC Malaria Test tube or draw v
7. be used provided the temperature DOES NOT EXCEED 53 C 126 F IMPORTANT The motor shaft shaft pins and attaching hardware must also be clean and dry before reassembly If the rotor is cleaned without removing it from the centrifuge not a recommended procedure wipe it with a cloth moistened with water only DO NOT USE THE ABOVE DILUTE BLEACH SOLUTION Do NOT wipe the rotor with any other cleaning agent since it cannot be sufficiently rinsed off while the rotor is installed 21 9 3 RELEASING LID LATCH If the lid is latched and there is a loss of main power to the centrifuge i e an interruption of primary electrical power the lid will remain latched A jammed plunger in the latch solenoid can also cause the lid to remain latched If you cannot wait for the restoration of power the lid can be opened as follows a Disconnect the Power Pack plug from the centrifuge b Turn the centrifuge upside down Using a Flat head screwdriver Y ener nm locate in the center the emergency lid 1 open slot c Push gently on the end of the solenoid plunger until the latch is released Figure 9 1 9 2 Figure 9 1 eap Figure 9 2 d Turn the centrifuge upright Reconnect the plug of the Power Pack to the centrifuge e When the power problem is corrected check by closing and latching the lid then turn power on and off to verify that the lid unlatches properly 22 9 4 TROUBLESHOOTING A referenc
8. A XE XXX seis casei FN R A EUER KG CN ERAN Edu RA E ERA REG 19 Section 9 MAINTENANCE AND SERVICE AAA 20 9 4 GENERAL CLEANING need dee dei nd e Kee Des de 20 9 2 ROTOR MAINTENANCE EE 20 9 2 1 Neel ee EE 20 9 2 2 Rotor Cleaning Procedures eech ie ee cett eee tete li epus LEE la ek Y deers ev xa haa E 21 9 3 RELEASING LID LATOCH nnne ennnnree nnns ERa et rara aea aana ad kaenda ranah didain rE a sen 22 9 4 TROUBLESHOOTING sssi ioira ea aa aa araea a a beac a Ea SEE sags KE ere EA Eaa NAE O RRE EE SEEE O EERE aa ERE 23 EK 25 WARRANTY e O 26 Section 1 INTRODUCTION 1 1 INTENDED USE The QBC Capillary Centrifuge Figure 1 1 is an ultra compact instrument designed to spin down blood specimens for QBC centrifugal hematology tests or the QBC Malaria Test Figure 1 1 QBC Capillary Centrifuge Model 425740 1 2 SUMMARY AND EXPLANATION 1 2 1 QBC Centrifugal Hematology Tests QBC centrifugal hematology tests are based on a high precision glass capillary tube containing an equally precise plastic float When the tube is filled with blood sealed and centrifuged at high speed for five minutes the float is buoyed by the packed red cells automatically positioning the float within the buffy coat layer between the packed red cells and the plasma QBC tubes for hematology are produced in a standard design which contain an unassembled float PLATELET LAYER and tube closure
9. ATOLOGY TESTS iion apais tate oati Eee paa iaa aena KES agorai 13 5 4 SPECIMENS FOR QBC MALARIA TEST rtt ea Euer ea ee E aaa REY RA REOR ER EAR RR RAE RR AS YRX RE REN eet 14 Table of Contents continued Section 6 CENTRIFUGATION PROCEDURES sees nnne nennen nnne nnne rne nnns 15 6 1 MATERIALS PROVIDED 1 2 rientra eene beu Da drp a eek Le del a EE Ra Ra Dua ex Ret LER e eu ERR NEESS BARRE RR Dees 15 6 2 MATERIALS REQUIRED BUT NOT PROVIDED esee nenne nnne nnne n trnn nennen ener a tnnt 15 6 2 1 OBC Centrifugal Hematology Tests iii i eet reet tr units na er XXn En cR FR ERAS ENEE ERR 15 ET ENK 15 6 3 TEMPERATURE REQUIREMENTS viii Lot Eoi ete deret o Lo o ood EHE eee et hind 15 6 4 CENTRIFUGATION INSTR GTIOES 2227222 raana geai poet ia red eterni Rede ee EE tees 16 6 41 Affixing Identification Labels niic toit ei rte etae Era et EEEREN E ERR deeg 16 6 4 2 Loading and Balancing ROOM gece eke r EEN ect cede ede ceca sete Cua tuse eto ena c Ee cta eae Y eX Phaser dux 16 0 4 3 e elt le Eeler 16 4 4 TESTING TDO re E O 17 Section 7 LIMITATIONS OF PROCEDURES sss nnne nenne tnnt risen rnn nennen nnne nennen 18 7 1 QBC CENTRIFUGAL HEMATOLOGY TEST 18 TQBO MALARIA MES EE 18 Section 8 CALIBRATION CHECKS ek 19 84 CHECKING ROTOR SBEED 2 texto Rt Eed fas laces sees EE caes RR REN SERE eR x ea ec 19 8 2 CHECKING TIMER ACCURAQGY it price reae eterna SNE saa RR REP E
10. CENTRIFUGE DESCRIPTION e M 4 Gil OW VIER HE 5 2 4 SPECIFICATIONS e ERE 5 2 4 1 QBC Capillary Centrifuge part no A2p 7401 5 2 4 2 OBC Power Pack part no 421763 sss nenne nnne nennen nenne seen n rnne senes 5 Section 3 INSTALLATION AND GET UP 6 one elem si cippEE 6 3 2 INSTALLATION AND GEDRWICE AA 6 3 3 CONNECTING POWER UNIT 5 5 2 xe ere heu REFER deca x pectin sias tes fhe e S EXRR SEE EENS S RENDER 7 3 4 PLACEMENT AND OPERATING ENVIRONMENT A 7 3 5 ROTOR ASSEMBUY EE 8 Section 4 CENTRIFUGE OPERATION AAA 9 4 GENERAL PROGEDURES eege nest EN eege isse a tds as teret EE eege 9 4 2 BEIWEEN SPIN TIME DELAY edel Bie 9 4 2 1 OBC Centrifugal Hematology TUDGS ecseeeceeeeseeeeeeeeeeeen eee ees saeeee eee saeeeeseeaaaeseesneeesesseseeeeeesneesenees 9 42 2 QBC Malaria Test EE 10 4 3 TUBE LOADING AND BALANCING eese nnne nnn nennen sette shirts nnne sen rnn snnt ns rites enn 10 4 4 EMERGENGCY UNLATCHING OF LID 2 453 eessen e usa ivy EY eg 11 4 5 SPEED POWER LED MONITORS suits cadillac iue ertet E deest ipe elise ie eae one Pe E Een ein 11 4 6 PRECAUTIONS AND HAZARDS nnne nnns enn nns entres entres es tnns entres sen rns sen ren seins rentre sen 12 Section 5 SPECIMEN COLLECTION 13 yd GENERAL esis 13 5 2 HANDLING PRECAUTIONS E 13 5 3 SPECIMENS FOR QBC HEM
11. FUGAL HEMATOLOGY TESTS QBC tests should be performed at temperatures between 20 and 32 C 68 and 90 F If tubes are run at temperatures below 20 C test results may be erroneously high if tested above 32 C interfaces may become blurred and difficult to detect visually or automatically depending on the model of QBC analyzer performing the test 7 2 QBC MALARIA TEST The QBC Malaria Test is a qualitative method for diagnosing positive or negative patients While numerous reports have demonstrated that the QBC Malaria Test is significantly more sensitive for detecting malaria than stained blood films the QBC Test may not detect very low levels of parasitemia Experienced microscopists can discriminate between various species of malarial parasites using the QBC Malaria Test Examination of a thin blood smear may be required to differentiate species in some cases See the QBC Malaria Test package insert for a list of literature references IMPORTANT For room temperature specifications consult the package insert of the disposables in use in your laboratory Section 8 CALIBRATION CHECKS According to Federal Regulations centrifuges such as the QBC Capillary Centrifuge require verification or calibration as follows 1 before initial use 2 after repair or adjustments and 3 annually after use The centrifuge timer however should be checked for accuracy at least every 3 months The QBC Capillary Centrifuge has been fully ca
12. ISTENT WITH THE MANUFACTURER S SPECIFICATIONS THE PROTECTION AGAINST HAZARDS THIS DEVICE PROVIDES MAY BE IMPAIRED Be sure to keep the operating area within the temperatures listed below For QBC Centrifugal Hematology tests operating temperature should be maintained at 20 to 32 C 68 to 90 F to assure proper cell layering in QBC blood tubes For QBC Malaria Test operating temperature should be maintained at 16 to 37 C 60 to 99 F to assure the concentration of parasitic organisms 3 5 ROTOR ASSEMBLY The QBC Capillary Centrifuge is shipped with the rotor assembly completely installed If the rotor is removed for example for cleaning see Section 9 2 it can be reinstalled as follows al Position the rotor so that the slots Motor on the underside of the rotor hub Drive Pin C are aligned with the drive pin in p the motor shaft Figure 3 4 D b Press the rotor down until the drive pin is seated in the hub slots and iiai 3 5 threads of the shaft protrude Pea a above the hub about 6 mm 4 CAUTION TO PREVENT DAMAGE TO ROTOR COVER AND CENTRIFUGE LID BE SURE ROTOR IS CORRECTLY SEATED ON DRIVE PIN c Thread the head nut onto the shaft finger tight by turning it clockwise Tighten with a light torque using the head nut wrench supplied with the centrifuge Figure 3 5 DO NOT OVERTIGHTEN Turn nut 1 2 tube positions to tighten f 2 4 z XX pA Figure 3 5 Tightening Rotor w
13. R CORD BEFORE CLEANING Use soap or a mild detergent and water to clean the lid housing and most parts of the QBC Capillary Centrifuge See below for special instructions on cleaning the rotor To prevent marring or scratching the finish avoid solvents or strong abrasives Dry all surfaces with soft paper towels or cloth Important When cleaning the inside of the centrifuge do not allow any fluids to run down into housing beneath the rotor Fluid in this area can damage the centrifuge Cleaning and decontamination may be necessary as a safeguard before the centrifuge or rotor is maintained repaired or transferred The user should attach a label or tag to the unit before it is moved or serviced certifying that cleaning and decontamination were performed If hazardous material is spilt on or inside the instrument it is the responsibility of the user to observe and follow appropriate decontamination procedures Handle all patient specimens as potential biohazards capable of transmitting infection When cleaning the instrument always wear appropriate personal protective equipment including laboratory gloves In addition to wearing gloves the use of disposable lab coats or gowns and protective glasses or goggles is recommended when working around the instrument 9 2 ROTOR MAINTENANCE 9 2 1 Periodic Inspection Remove and inspect the rotor for defects at least every 12 months and each time the rotor is removed for cleaning To
14. TIONS 2 4 1 QBC Capillary Centrifuge O Rotor Capacity 1 to 20 QBC tubes O Rotor Speed 12 000 rpm 80 rpm O Nominal Relative Centrifugal Force RCF 14 387 x g O Timer Electronic 300 s spin 15 20 s deceleration O Electro Mechanical Safety Interlock Lid must be closed and latched before motor can be energized Lid remains locked until rotor stops O Operating Temperatures For QBC Hematology 20 to 32 C 68 to 90 F For Parasite Detection 16 to 37 C 60 to 99 F O Functional Range Centrifuge Operation 2 to 40 C 36 to 104 F O Operating Relative Humidity 0 to 8096 for temperatures between 2 and 31 C derating linearly to 5096 between 31 and 40 C L1 Non Operating Storage Temperature Limits 26 to 66 C 15 to 150 F L1 Weight with rotor and cover installed 2 8 kg 6 2 Ibs L1 Dimensions with lid closed 23 5 cm W x 29 8 cm D x 12 7 cm H 9 25 in W x 11 75 in Dx 5 in H O Transient Overvoltage Category I L1 Indoor or under cover use only O Pollution Degree Il O Electrical 48 VDC 3 VDC 2 7 ADC Maximum Steady State 2 4 2 QBC Power Pack The power supply accompanying or sold with the QBC Capillary Centrifuge is recognized under UL 60950 1 CSA C22 2 No 60950 1 2006 95 EC Low Voltage Directive and 2004 108 EC EMC Directive L1 Input 100 240 VAC 47 63 Hz O Output 48 3 VDC 2 7 amp O DC Output Cord L1 AC Line Cord unassembled e Standard Cord 2 3 m 7 5 ft e Continental Europ
15. US DISEASES HANDLE BLOOD AS A POTENTIAL BIOHAZARD CAPABLE OF TRANSMITTING INFECTION ALWAYS WEAR GLOVES AND OTHER APPROPRIATE BLOOD BARRIER PROTECTION WHEN COLLECTING AND PREPARING BLOOD WARNING QBC TUBES ARE MADE OF GLASS EXERCISE CARE WHEN HANDLING AND PREPARING TUBES TO PREVENT BREAKAGE AND POSSIBLE INJURY Consult Section 4 6 of this manual for detailed safety precautions IMPORTANT The coating ingredients in QBC tubes for hematology differ from those in the QBC Malaria Test tubes Accordingly do not interchange QBC hematology tubes with QBC Malaria Test tubes since erroneous test results will be obtained 1 5 SYMBOL DEFINITIONS Direct Current non alternating e A Not for use in the presence of flammable gases Lo Attention Read the attached note Section 2 SYSTEM DESCRIPTION 2 1 COMPONENTS The QBC Capillary Centrifuge is shipped with an external plug in power pack that supplies the required direct current D C voltage to power the motor circuit board and other internal systems of the instrument 2 2 CENTRIFUGE DESCRIPTION The QBC Capillary Centrifuge is a compact low profile unit measuring less than 13 centimeters 5 1 in high with cover down and housed in a sturdy plastic case As shown in Figure 2 1 the centrifuge incorporates a 20 place rotor mounted to the shaft of a brushless D C motor In addition to shock mounting of the motor to minimize vibration the centrifuge rests on four suction fe
16. d is spring loaded and is automatically released when the Power switch of the centrifuge is off provided the Power Pack is on and connected to the centrifuge When a spin cycle is completed the latch releases after a momentary delay and the lid pops up Figure 4 2 Place tubes on the rotor in a balanced array with the tube closures against the outer rim of the rotor To maintain balance insert the tubes consecutively starting at slot 1 When spinning an odd number of tubes e g three BE SURE TO BALANCE THE ROTOR BY PLACING AN UNFILLED QBC TUBE IN THE OPPOSITE SLOT e g 4 as shown in Figure 4 3 Use a closure and a float in the balance tube Figure 4 3 Typical Placement of Blood and Balance Tubes 4 3 TUBE LOADING AND BALANCING continued Before closing the lid INSTALL THE ROTOR COVER To secure the cover turn the cover knob clockwise onto the motor shaft Figure 4 4 Continue turning the knob until the cover is snug DO NOT OVERTIGHTEN CAUTION NEVER ATTEMPT TO CENTRIFUGE QBC TUBES UNLESS THE COVER IS INSTALLED OVER THE ROTOR SINCE TUBE BREAKAGE AND LOSS OF SAMPLE WILL OCCUR i gt Figure 4 4 Tightening Knob of Rotor Cover t 4 4 EMERGENCY UNLATCHING OF LID An electric interlock prevents the operator from manually opening the centrifuge lid once it is closed and latched In case the latched lid must be opened and the cover was installed over the rotor press the ON OFF button twice t
17. e Power Pack and the male plug to a grounded AC power receptacle Figure 3 2 Connecting Power Pack Output Figure 3 3 Power Pack and AC Line Cord Cable to Centrifuge Power Input Cable CAUTION To avoid electrical hazards and equipment damage connect the power cord only to grounded receptacles delivering the voltage and frequency specified on the data plate of the power supply with a switch or other means of circuit disconnection located outside the clearance envelope defined in Section 3 4 of this manual When only a 2 wire receptacle and or an unswitched line is available have the receptacle and or the supply line modified by a qualified service technician in accordance with applicable electrical codes to conform to this note 3 4 PLACEMENT AND OPERATING EQUIPMENT Place the centrifuge on a smooth level working surface in a location where it will not be exposed to direct sunlight Centrifugations in direct sunlight can elevate the rotor compartment temperature and may adversely affect the formation of readable cell layers in QBC blood tubes For stability be sure the unit rests on its suction feet Mark an envelope 300 mm 12 inches wider than the body of the centrifuge around the area in which the centrifuge is to be used Keep all personnel and hazardous materials outside this envelope when the centrifuge is operating 3 4 PLACEMENT AND OPERATING EQUIPMENT continued WARNING IF THIS EQUIPMENT IS USED IN A MANNER INCONS
18. e guide to common operating problems with the centrifuge is presented below PROBLEM CAUTION ACTION Centrifuge shows no sign of power but primary electrical supply is available Loose power connection Lid is not latched Centrifuge does not operate when the ON button is pressed Failure of micro switch in latch mechanism Centrifuge rotor is spinning Centrifuge lid will not open but primary electrical supply is available Latch release delay Plunger of latch solenoid is jammed Press power button OFF to stop rotation See Action steps Centrifuge vibrates excessively Note momentary vibration is normal during acceleration but should disappear upon reaching full speed Note Centrifuge is capable vibrations 23 of 30 tilt without causing excessive Check all power plugs Close and latch lid Request service An electrical interlock prevents the lid from opening until rotor stops spinning Wait until all motion ceases Wait 4 5 s after rotor stops See Section 9 3 and release solenoid plunger a Be sure tube load is properly balanced b Tighten rotor nut with wrench replace and tighten rotor cover Close top lid and re check for vibration C Check that surface does exceed 30 tilt limit 9 5 TROUBLESHOOTING continued Troubleshooting Guide for 425740 Centrifuge Power On Yellow Light Check all Connections Lid Won t Open Green Light
19. ean Cord 2 5 m 8 2 ft For temperature specifications consult the package insert of the disposables in use in your laboratory Section 3 INSTALLATION AND SET UP 3 1 PACKAGING AND PARTS The QBC Capillary Centrifuge and Power Pack are shipped in a single container together with the following parts test accessories and instructions Figure 3 1 Rotor Cover Assembly Head Nut Wrench e Two Power Pack Line Cords see below e Rotor Removal Wire Operating Instructions this Manual Note For use of the centrifuge in Continental Europe an alternate line cord is provided with the Power Pack Select the appropriate line cord and discard the unused cord 3 2 INSTALLATION AND SERVICE For first time users of the QBC System a representative of QBC Diagnostics Inc or your local QBC System distributor will normally install the required equipment the installer will also train operating personnel in proper use of the equipment If necessary contact your nearest QBC Diagnostics Inc office to arrange for installation service Figure 3 1 QBC Capillary Centrifuge Showing Parts and Accessories Note Centrifuge is supplied with rotor installed 3 3 CONNECTING POWER UNIT Be sure the Power Pack is not plugged in Connect the power pack output cable plug to the power input cable on the right side of the centrifuge Figure 3 2 Using the appropriate line cord Figure 3 3 connect the female plug to the receptacle in the side of th
20. ed in the QBC Malaria Test tube promote separation and metachromatic staining of these cells GRANULOCYTE LAYER LIGHT RED CELLS LYMPHOCYTE MONOCYTE LAYER ES PLATELET LAYER DARK RED White Label CELLS od PRIMARY MALARIA FLOAT N SECONDARY MALARIA DETECTION ZONE DETECTION ZONE 55 65 uL Blue Fill Lines Figure 1 3 Differentiated Cell Layers in Spun QBC Malaria Test Examination of the centrifuged blood under a fluorescence microscope or a light microscope with the QBC ParaLens Advance LED fluorescence microscope attachment readily permits the detection of malaria in the infected cells and plasma Direct malaria detection by the QBC Test method is therefore more rapid than conventional blood film microscopy 1 3 PRINCIPLES OF THE PROCEDURES 1 3 1 QBC Centrifugal Hematology Tests The QBC Capillary Centrifuge is designed to spin up to 20 QBC blood tubes at the force time requirements needed to produce optimally packed cell layers for cell counting and analyses QBC blood tubes are 75 mm glass tubes internally coated with potassium oxalate acridine orange anticoagulants and an agglutinating agent The tubes are sealed with a plastic closure or rubber stopper and contain a precision molded float within the blood filled tube Rotor speed and timing of the centrifuge are computer controlled for maximum consistency in the formation of packed cell layers in spun QBC tubes 1 3 1 QBC Centrifugal Hematology Tests continued
21. enous blood and collect into the tube Fill the tube with 55 to 65 uL of blood QBC Malaria Test tubes are pre coated with sodium heparin and dipotassium EDTA anticoagulants Stability Centrifuge the blood filled QBC Malaria Test tubes as soon as possible after filling See 6 4 4 for extended stability of the QBC Malaria Test prior to microscopic examination Section 6 CENTRIFUGATION PROCEDURES 6 1 MATERIALS PROVIDED The QBC Capillary Centrifuge is designed to spin up to 20 QBC type blood tubes over a fixed time of 5 min The centrifuge is supplied with a Power Pack and the test accessories listed in Section 3 1 6 2 MATERIALS REQUIRED BUT NOT PROVIDED 6 2 1 QBC Hematology Tests In addition to a QBC Reader or Analyzer the following disposables and test accessories are required for QBC hematology tests Accessory Part No QBC Venous Tubes Box of 100 424240 e QBC Venous Tubes Box of 1000 424237 QBC Capillary Tubes Box of 100 424241 e QBC Capillary Tubes Box of 1000 424238 1 8 mm Blade Lancets Box of 100 420000 e 2 3 mm Blade Lancets Box of 100 420001 Work Station 424226 e Sterile wipes 6 2 2 QBC Malaria Test The following disposables and test accessories are required for the QBC Malaria Test Accessory Part No e QBC Malaria Test Box of 100 253037 e QBC Malaria Test Box of 1000 253005 e QBC ParaLens Advance with 60x Objective 424331 e QBC Microscope 424294 e Work Station 424226 e Sterile wipes 6
22. equires that the assembly be cleaned only by the procedures and with the approved agents specified in 9 2 2 Before using any cleaning or decontamination methods other than those recommended by the manufacturer users should check with the manufacturer that the proposed method will not damage the equipment 9 2 2 Rotor Cleaning Procedures To clean always remove the rotor from the centrifuge so that the procedure can be performed properly a Cleaning Agents The rotor assembly can be damaged by certain chemicals and disinfecting agents Clean and disinfect the rotor only with a solution containing a 1 10 dilution of commercial sodium hypochlorite 596 CAUTION DO NOT USE ANY OTHER CLEANING AND DISINFECTING AGENTS SINCE THEY CAN CORRODE AND DAMAGE THE ROTOR BE SURE TO THOROUGHLY RINSE AND DRY THE ROTOR BEFORE RE INSTALLING A 1 10 dilution is prepared by adding one 1 part of household bleach to nine 9 parts of water Soak the rotor in the dilute bleach for at least ten 10 min to destroy any viral and bacterial contaminants BE SURE TO REMOVE THE ROTOR PROMPTLY AFTER 10 MIN OF SOAKING IN THE BLEACH SOLUTION SINCE EXTENDED EXPOSURE TO THE BLEACH CAN CORRODE THE ROTOR b Rinsing and Drying After soaking in the dilute bleach solution specified above completely immerse the rotor in clean water Rinse again under running water to remove all traces of bleach Thoroughly dry the top and bottom surfaces of the rotor Oven drying may
23. et for added stability A protective metal cover which threads onto the motor shaft keeps the spinning tubes snugly in place on the rotor The hinged top lid with safety latch provides a housing or shield around the rotor during operation Electrical power to the centrifuge is controlled by a momentary ON OFF power button located on the front deck Before the motor will start the top lid must be closed and latched Once a spin cycle has started a safety interlock prevents the lid from being opened until the rotor brakes to a stop The spin cycle is controlled by an electronic timer on the main circuit board Spin time is fixed at 5 min ke Lid Latch Rotor Cover ESS Lid Assembly Power SE Input Cable x ON OFF Head Nut Button Safety Interlock p Green Switch gege Speed LED Yellow Kee Power LED Figure 2 1 Features and Controls of QBC Capillary Centrifuge 2 2 CENTRIFUGE DESCRIPTION Continued A green light emitting diode LED for SPEED and a yellow LED for POWER adjacent to the ON OFF button provide visual indicators of power circuit status Condition of each LED On Off or Flashing based on the status of power lid latching rotor speed etc is described in Sections 4 5 and 9 5 2 3 POWER PACK The Power Pack for the QBC Capillary Centrifuge is a table top 130 watt switched mode power supply with a universal input range 100 240 VAC and a 48 VDC output 2 4 SPECIFICA
24. illary Centrifuge QBC Diagnostics Inc warrants the QBC Capillary Centrifuge to be free from defects in workmanship and materials for a period of one 1 year from date of installation provided the centrifuge is operated in accordance with the Operator s Manual During such period QBC Diagnostics Inc agrees to replace or repair any parts which in its judgment are found to be defective provided the centrifuge has not been subjected to misuse or abuse The warranty stated herein shall extend to the original consumer and not to any subsequent consumer of the centrifuge QBC Diagnostics Inc shall not be liable for any incidental or consequential damages QBC Diagnostics Inc makes no other warranties expressed or implied except as stated herein National Contact QBC Diagnostics 168 Bradford Dr Port Matilda PA 16870 U S A Voice 1 814 692 7661 Fax 1 814 692 7661 Toll Free Technical Services 1 866 265 1486 Customer Service 1 877 231 3115 www qbcdiagnostics com 26
25. ith Head Nut Wrench Section 4 CENTRIFUGE OPERATION 4 1 GENERAL PROCEDURES Before starting the QBC Capillary Centrifuge be sure the rotor cover is installed and the lid is closed and latched The centrifuge will not start unless the lid is latched e To start the 5 min spin press and release the ON OFF button on the front deck of the centrifuge Figure 4 1 The rotor will begin accelerating until it stabilizes at the design speed See Section 4 5 for sequencing of POWER and SPEED indicator lights CAUTION TO PREVENT PERSONAL INJURY OR DAMAGE NEVER LEAN ON PICK UP OR TILT THE CENTRIFUGE WHILE IT IS POWER OPERATING NEVER REMAIN WITHIN THE CLEARANCE ENVELOPE DEFINED IN SECTION 3 4 OF THIS MANUAL LONGER THAN NECESSARY FOR OPERATIONAL REASONS Figure 4 1 Detail of ON OFF Button and POWER SPEED LED s e Spin time is fixed at 5 min and is not adjustable After 5 min spin 15 20 s are required for the rotor to decelerate to a full stop at which time the lid latch unlocks To stop the centrifuge any time during a spin press and release the ON OFF button Note After completing any spin cycle full or interrupted the timer is automatically reset to zero 4 2 BETWEEN SPIN TIME DELAY Since the QBC test method depends upon proper cell layering in the hematology and QBC Malaria Test tubes blood sampled must be protected from excessive heat buildup in the rotor compartment which may adver
26. librated at the factory and is ready to deliver consistent accurate test results upon first use For subsequent calibration checks follow the procedures in 8 1 and 8 2 below 8 1 CHECKING ROTOR SPEED The rotor of the QBC Capillary Centrifuge is designed to spin at 12 000 80 rpm At least every 6 months or after any adjustments or repairs check the rotor speed with a non contact strobe tachometer to assure that speed is sufficient to produce visibly distinct and differentiated cell layers in the QBC tube A window in the top lid and a reference line on the knob of the rotor cover are provided for speed checks with a non contact tachometer 8 2 CHECKING TIMER ACCURACY The electronic timer of the Centrifuge is designed to be accurate to 300 15 s Check the timer for accuracy against a reliable stopwatch or quartz timer at least every 3 months as follows al Install the rotor cover b Close the latch and top lid C Press and release the ON button and simultaneously start the stopwatch d Stop the stopwatch when you audibly detect cutoff of power to the motor and the centrifuge begins to decelerate e The stopwatch reading should be 5 min 15 s If the 15 s tolerance is exceeded repeat the check to eliminate the possibility of procedural errors Request service if the accuracy check is unsatisfactory Section 9 MAINTENANCE AND SERVICE 9 1 GENERAL CLEANING CAUTION TO AVOID ELECTRICAL HAZARDS ALWAYS DISCONNECT THE POWE
27. o start and stop the Centrifuge The lid will then open In case the latched lid must be opened but the rotor cover was inadvertently left off DO NOT attempt to start and stop the centrifuge with the ON OFF button instead unplug the Power Pack and wait for the yellow LED to turn off Plug the Power Pack back in This will disengage the electric latch interlock and cause the lid to open Note For procedures on opening the lid in the event that main power to the centrifuge is lost through a fuse or main electrical failure see Section 9 3 4 5 SPEED POWER LED MONITORS The yellow POWER and green SPEED LED lights are used for monitoring the status of the centrifuge When both lights are on steadily the centrifuge is at design speed 12 000 80 rpm When the yellow light is on steadily but the green light is flashing the centrifuge is below but accelerating to design speed and when both lights are flashing the unit is above design speed If speed exceeds 14 800 rpm the centrifuge will automatically shut down A detailed table of LED Status Indicators is presented in Section 9 5 TROUBLESHOOTING 4 6 PRECAUTIONS AND HAZARDS The QBC Capillary Centrifuge is intended for in vitro diagnostic tests In order to obtain properly centrifuged specimens and to avoid damage or hazards the basic operating precautions below should be observed For smooth operation and long service life always place blood tubes on the rotor in a balanced ar
28. r with an empty QBC hematology tube or QBC Malaria Test tube Note Do not attempt to re use the balance tube for patient samples 6 4 3 Centrifugation Procedures a Secure the cover to the rotor then close and firmly latch the lid b Press and release the ON button to start the spin cycle c After the five min spin cycle is complete and the lid pops up open the lid and REMOVE THE TUBES IMPORTANT Failure to remove tubes promptly after centrifugation may affect cell layer boundaries and subsequent test results d If not read promptly the centrifuged tube should be stored temporarily stopper or closure end down in the QBC work station Note See Test Timing in 6 4 4 6 4 4 Testing Time Testing of QBC hematology tubes and QBC Malaria Test tubes promptly after centrifugation is recommended The delays below however will not affect test results e QBC Hematology Tubes To obtain accurate cell counts test hematology tubes in the QBC Analyzer 4 hours after centrifugation Store the tubes vertically stopper down in the tube rack away from heat and intense light Consult the applicable QBC System Operator s Manual for test instructions e QBC Malaria Test After centrifugation QBC Malaria Test tubes can be held for as long as 3 days unrefrigerated at temperatures up to 37 C 99 F before microscopic examination Store tubes vertically closure down in the tube rack Section 7 LIMITATIONS OF PROCEDURES 7 1 QBC CENTRI
29. ray Always install the rotor cover before closing the top lid Never attempt to override the lid safety interlock Never attempt to pick up or move the centrifuge while the rotor is spinning Universal Precautions should be followed in handling all items contaminated with blood or other body fluids Exercise care when using QBC tubes The tubes are made of glass and may break possibly causing injury and exposure If a tube breaks carefully remove broken glass with a hemostat or other device using puncture resistant utility gloves and dispose of properly Disinfect rotor after a tube break See Section 9 2 2 For continued safety periodically inspect the rotor for signs of wear or defects as described in Section 9 2 of this manual Blood products and other biological materials should always be disposed of in accordance with laws regulations and institutional guidelines applicable to your facility Recommendations for Prevention of HIV Transmission in Health Care Settings MMWR 1987 36 Supplement 25 Section 5 SPECIMEN COLLECTION 5 1 GENERAL The QBC Capillary Centrifuge is designed for the preparation of specimen tubes for QBC hematology and the QBC Malaria Test Detailed directions on blood collection preparation and stability for each type of test are contained in the package insert supplied with QBC hematology tubes and the QBC Malaria Test Handling precautions and collection procedures are summarized below 5 2
30. remove the rotor 8 Turn the head nut counterclockwise with the wrench to remove b Insert the removal wire under the rotor Lift both sides of the wire to pry up the rotor Figure 9 1 Figure 9 1 Prying Rotor From Motor Shaft with Removal Wire Carefully inspect the rotor for defects that could impair its continued safe use Check top and bottom surfaces for the following 20 Cracks especially hairline cracks in the region adjacent to the hub e Surface corrosion particularly in the region of the hub Deep scratches or severe dents If any of the above defects are found replace the rotor with a new assembly See Section 9 6 for catalog numbers Also inspect the motor shaft shaft pin and head nut for signs of wear corrosion or damage Replace any defective parts or request repair service Use the centrifuge only if all parts are in good condition 9 2 1 Periodic Inspection continued To replace the rotor refer to Figures 3 4 and 3 5 Position the rotor hub on the motor shaft so that the slots on the underside of the hub are in line with the shaft pin Press the rotor down until the shaft pin is seated in the hub slots and 3 5 threads on the shaft protrude above the hub about 6 mm 4 Secure the rotor to the motor shaft with the head nut Lightly tighten the nut with the wrench Also inspect the centrifuge housing and lid for cracks missing pieces or deformations Extended service life of the rotor r
31. sely affect cell layer formation Accordingly between successive spins be sure to wait the appropriate time specified below IMPORTANT During each between spin rest be sure the rotor cover is removed and the rotor empty 4 2 1 QBC Hematology Tubes e f room temperature is 24 C or less x 75 F wait 1 min between spins e f room temperature is 25 28 C 75 82 F wait 3 min between spins e f room temperature is 29 30 C 84 86 F refer to the table below Between Between Between Between 1st amp 2nd Spins 2nd amp 3rd Spins 3rd amp 4th Spins 4th amp 5th Spins stadia hes e If room temperature is 31 32 C 88 90 F wait 30 min between spins Note During QBC Hematology tests using standard QBC tubes room temperature should not exceed 32 C 90 F Important For room temperature specifications consult the package insert of the disposables in use in your laboratory 4 2 BETWEEN SPIN TIME DELAY continued 4 2 2 QBC Malaria Test e f room temperature is less than 33 C lt 91 F wait 1 min between spins e f room temperature is 33 35 C 91 95 F wait 3 min between spins e f room temperature is 36 37 C 97 99 F With 1 to 5 consecutive spins wait 6 min between spins With consecutive spins above 5 wait 9 min between spins Note During use of QBC Malaria Test room temperature should not exceed 37 C 99 F 4 3 TUBE LOADING AND BALANCING The top li
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