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Contents
1. 1 0 16S rDNA 7 1 1 bp eaeA 384 1 Reaction stx1 132 32 mixture 1 stx2 93 64 Reaction est 130 16 mixture 2 elt 62 8 aggR 575 2 Reaction invE 382 4 mixture 3 16S rDNA 120 Reaction mixture 1 00 P 3 4 1 10 100 100 bp P F 1 ATCC 35150 EHEC 2 ATCC 43887 EPEC 3 ATCC 35401 ETEC 4 ATCC 43893 EIEC 5 ATCC 25922 6 10 39 KANTO HC 1 x TBE Mupid exU 100 V 35 13 8 pl2. stx2f 4 ME 5 EIEC 36 6 20 C 7 PCR 8 103 0022 2 2 1 03 6214 1090 541 0048 2 5 1 812 0007 2 22 3 11
3. DNA 08203 96 100 DNA 01016 96 AptaTaq DNA Master 500 ul 24080 96 6 2mlx6 24079 96 6 2mlx6 G 72089 STEC 1L EHEC 72090 STEC 10 EHEC 71755 5 EHEC 12 KALS 2008 283895 2 Ruttler M E et al oce 30 2 301 308 2006 3 Hiroe A et al 4pp Environ Microbiol 78 9 3177 3184 2012 http www kanto co jD e mail reag info gms kanto co jp 06 6231 1672 092 414 9361
4. DNA DNA Cec 103 0022 03 6214 1090 541 0048 0 812 0007 2T 22 3 DNA DNA 08178 96 DNA DNA 1 DNA 100 2 10 pl 1 3
5. 3 72 C 6 94 3 4 15 000rpm 1 DNA PcR 3 C D E 1 3 PCR F DNA PCR Reaction mixture 1 DNA DNA 5 0 ul A AptaTaq DNA Master 4 0 pl B PCR 7 0 ul 1 4 0 pl 20 0 pl Reaction mixture 2 DNA DNA 5 0 ul A AptaTaq DNA Master 4 0 ul B PCR 7 0 ul D 2 40 ul 20 0 pl Reaction mixture 3 DNA DNA 5 0 ul A AptaTaq DNA Master 4 0 ul B PCR 70 ul EE 3 4 0 ul 20 0 pl PCR
6. bp 1 1 2 3 4 5 6 7 8 9 10 ege4 384 1 1 1 0 0 0 1 1 0 0 0 Reaction s 7 132 32 1 0 0 0 0 1 0 0 0 0 mixture 1 stx2 93 64 1 0 0 0 0 1 0 0 0 0 Reaction est 130 16 0 0 1 0 0 0 0 1 1 0 mixture 2 ekt 62 8 0 0 1 0 0 0 0 1 0 0 aggR 575 2 0 0 0 0 0 0 0 0 0 1 Reaction invE 382 4 0 0 0 1 0 0 0 0 0 0 mixture 3 16SrDNA 120 1 1 1 1 1 1 1 1 1 1 97 1 24 4 0 97 1 24 16 2 0 EHEC EPEC ETEC EEC EHEC EPEC ETEC ETEC EAEC STEC STEC 4 0 EHEC STEC EPEC ETEC EIEC EAEC 1 EPEC 2 EAEC 4 7 EIEC 10 8 11 ETEC e 16 lt 19 ETEC est 24 27 ETEC est eK 32 35 EHEC STEC s 7 64 67 EHEC STEC s 2 96 99 EHEC STEC stx7 s2 10 1 DNA 10 100 ng ul 2 DNA 1 DNA 3 sp 2 2
7. 500 g 1 5 ml UV 0 2 ml PCR 1 5 ml 20 ul 200 pl 5 PCR 6 EHEC STEC EPEC ETEC EIEC EAEC 7 DNA
8. 13 3 9 2 1 0 3 4 16S rDNA PCR 2 1 1 ATCO 35150 bp eaeA 384 1 x 1 1 Reaction stx1 132 32 x 1 32 mixture 1 stx2 93 64 x 1 64 Reaction est 130 16 x 0 0 mixture 2 elt 62 8 x 0 0 aggR 575 2 x 0 0 Reaction invE 382 4 x 0 0 mixture 3 16S rDNA 120 1 97 97 EHEC STEC sw ss Cec Diarrheagenic E Coli 3 1
9. 4C 94 C 60 15 a 30 3 1 1xTBE 3 2 PCR 4 ul G 6 x 3 6 ul 4 100 V 35 100 bp DNA Ladder 0 5 ug ml 30 UV http www kanto co jp e mail reag info gms kanto co jp 6 6231 1672 092 414 9361 1 T7408 1 26 1 PCR Cica Geneus Pathogenesis Gene Detection PCR Kit for D 8
10. T7408 1 9 26 1 PCR Cica Geneus Pathogenesis Gene Detection PCR Kit for Diarrheagenic E Coli 1 Diarrheagenic Escherichia coli 7 EHEC EPEC ETEC EIEC EAEC DAEC EAST1EC PCR 5 EHEC EPEC ETEC EIEC EAEC 2 PCR Cica Geneus Pathogenesis Gene Detecto
11. n PCR Kit for Diarheagenic cg 08087 96 100 20 3 100 A AptaTaq DNA Master 5 x Conc 1 200 pl x 1 B PCR 1 100 lx 2 C 1 400 ul x1 D 2 400 ul x1 E 3 400 ul x1 F 400 ul x1 G 6 1 200 pl x 1 IAptaTaq DNA Master Roche Diagnostics K K 4 08178 96 DNA 120 DNA 01089 23 KANTO HC 100g 46510 79 10xTBE 1L 14575 43 2mg ml 10ml 49881 00 100 bp DNA Ladder 100 711129 5 500 g 711131 5
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