IO テスト PE 標識抗体 CD16 ION16
Contents
1. 1O o CD20 Nalm 6 1 Raii 2 95 CD20 JURKAT 3 0 5 1 Nm 6 pre B ALL 2 Raji Burkitt lymphoma 3 JURKAT T cell Leukemia PBS 4 CD3 HPB ALL CCRF CEM 3 10 3 5 52. 1 2 10 11 1 20uL 1 PBS PBS 6603369 1 500mL pH 7 2 0 2 BSA 0 5 0 1 2 PBS VersaLyse PBS 1mL IOTest 3 A07800 12 5uL 0 1 PBS VersaLyse
3. 29 EPICS XL vs PE 5 6 7 8 9 1 Leukocutes Lumphocutes 8B9g1 999 Count SS 6 gg 488 29g 1 1 18 108 19 9 lt 18 108 1 9 PE PE CYTO TROL 6604248 Fc lOTest CD45 FITC CD14 PE A07738
4. 4 5 Ficol Paque No 8 9
5. 1 25C 500xg 5 2 3 4 DxH800 5 PBS 5 10x103 mm 1 12mmex75mm 2 100uL 3 20uL IOTest Mouse lgG1 PE 20uL 4 15 5
6. IOTest T B NK 2 2
7. 6 PBS 2mL 7 400xg 3 8 6 7 9 0 mL 0 5 PBS F 10 24 3 Ficoll Paque 1 3 4mL PBS 2 Ficol Paque 4mL 1 3 2 8C 400xg 30 4 Ficol Paque PBS 2 8 C 400xg 8
8. PBS 1mL IOTest 3 A07800 67 5uL 0 5 PBS 3 A09777 VersaLyse IM1400 OptiLyse B A11895 OptiLyse C A07799 IOTest 3 4 IOTest Mouse IgG1 PE A09142 100 2mL 5 Ficoll Paque Phamacia 17 0840 03 2 1 100uL 5x103 mm3 10x103 mm3 PBS 3x103 mm3
9. A07766 Wa COULTER 2012 10 1 20200EZY00111000 I NK IO PE CD16 ION16 1 2 3 4 IO PE CD16 ION16 PE lgG CD16 50 65kD 48kD CD16 lgG Fc FcoRU NK 50 65kD 48kD
10. GPD CD16b 2 CD16 NK ADCC 3G8 GPI CD16 3G8 IgG1 PE Phycoerythrin 486 575nm 560 590nm CD16 CD16 PE 1 IO 2 EDTA 3 6
11. 7 0 5 1mL 0 5 PBS 8 24 FS 90 SS PE Log 29
12. PBS 2 8 C 400xg 4 PBS 2 8 C 400xg 3 0 PBS 5x103 mm3 5x109 mL 11 KS 90 1 12mmex75mm 2 Ficol Paque 5x10 5x10 mL 100uL 3 20uL IOTest Mouse lgG1 PE 20uL 4 2 8 C 30 5 PBS 2mL 2 8C 400xg 5 6 5
13. 2 8 C 12 IO PE CD16 ION16 A07766 100 2mL Whiteside T L Herberman R B The role of natural killer cells in immune surveillance of cancer 1995 Cur Opin Immunol 7 704 710 2 Hannet 1 Erkeller Yuksel F Lydyard P Deneys V DeBruyere M Developmental and maturational changes in human blood Ilymphocyte subpopulations 1992 Immunol Today 6 13 215 218 3 Robertson M J Ritz J Biological and clinical relevance of human natural killer cells 1990 Blood 12 76 2421 2438 4 Ritz J Trinchieri G Lanier L L NK cell antigens section report 1995 Leucocyte Typing V White Cell Differentiation Antigens 13276 Marseille Cedex 9 France SS Oh OM CO 135 0063 5 7 TOC TEL 0120 566 730 FAX 03 5530 2460 135 0063 5 7 TOC A07766_1210
14. 1 0 19
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