T8-RD1
Contents
1. T T Harvard Medical School Dr S F Schlossman Coulter Immunology T B Be 3 T B DT2. B T lg T B E Smlg E T T Smlg B lg Fc lg Smg T B
3. 9 2596 amp 8 2 1L 8 26g 1 0g EDTA4Na 2Na 37mg pH 7 2 7 4 1 1 2 30 2 10 15 E 20 30 MslgG1 RD1 T8 RD1 FA 6602884 100 0 5mL gt Propidium lodide Calbiochem 53705 0 01mg mL 0 05mg mL al Acridine orange Baker A366 3 0 005mg mL
4. CD8 Fi class MHC T8 SFCI21Thy2D3 CD8 BALB cJ NS 1 AG4 lg VIA lgG1 H RUKL E T8 RD1 RD1 Phycoerythrin PE RD1 0 51 5 486 575nm 568 590nm 1 0 5mL 0 2 0 01M 0 15M 0 1 T T CD8 488nm
5. 5 200L MSlgG1 RD1 200L 6 2 8 C 30 7 2 8 C 400 450 x g 5 8 3 4 9 PBS 200L MA 2 8 C CT 400 450 x g 5 10 3 4 11 PBS 200 u L 3 6 EPICS Cytomics 1 2 Ficoll Paque
6. gt PBS PBS 2 8 C NBS 296 BSA 0 196 0 1 lt T8 RD1 gt 1 Sul OMAR 5 L 195L PBS PBS PBS 6603369 1 500mL pH 7 240 2 0 196 a n 1 2 1 6603152 300 PBS 24mL 1mL
7. 3 RD1 PE Log 1 CD8 CYTO TROL 6604248 Fc Fc MSlgG1 RD1 1 lt 296 296
8. 7 Fc 10 15 1mg mL 8 9 10 11 T8 RD1
9. MSlgG1 RD1 200uL 5 K HL 2 8 CE 30 6 PBS 1mL EMMA 2 8 C EC 400 450 xg 4 7 5 2 8 0 5 1mL PBS 9 EPICS Cytomics 3 Ficoll Paque 1 1 x 10 200L Ficoll Paque U E 96 200L 2 2870 T 400 450 x g 5 3 4
10. B Epstein Barr CD8 T4 T8 CD4 CD8 CD4 CD8 T4 T8 T T T4 T8 T4 T8 CD4 ARC AIDS related complex AIDS AIDS CD4 T4 T8 LEAS 0 CD8 T4 T8 CD4 CD8 MS SLE
11. 3 4 LH 700 5 10 x103 mm 1 100L 2 Ficoll Paque 1 4mL PBS 2 Ficol Paque 4mL 1 3 2 80 T 400 450xg 30 4 Ficoll Paque 5 2 lt 8 C PBS 2 8 C 400 450 xg 8 6 PBS 7 2 80 T 400 450 x g 4 8 PBS
12. 3 859 4 Ficoll Paque Ficoll Paque 5 5 Ficol Paque 6
13. RD1 1 6 FS 90 SS 1 EDTA 6 2 90
14. gt 1 1 2 100L 3 200L MSlgG1 RD1 200L 4 45 5 a b a QL 5 15 b PBS 2 3mL 400450 xg 5 PBS 25 1mL
15. 9 2 80 T 400 450 x g 3 10 PBS 1x10 mm 1 x 10 mL 11 a b 90 a 12 ERIS 1x 10 1x 10 mm 1mL 13 2 8 C PBS 2 8 C 400 450 xg 4 14 0 05mg mL Propidium lodide 3 MA CHEL 1 15 2 8C PBS 2 8C 400 450 xg 4 16 15 17 85 b
16. 6603274 2009 11 2007 2 20200EZY00131000 877429 T T8 RD1 i m wo gt RD1 Phycoerythrin PE 1 T8 RD1 1 0 5 04g r AF T8 CD8 76kD 32 34kD CD8 8096 ERA MO T 30 lt 359 CD8
17. T8 RD1 2 8C 3 20 25 4 T8 RD1 T8 RD 6603274 BE 100 0 5mL 1 McMichael AJ ed Leukocyie Typing Ill White Cell Differentiation Antigens 1987 Oxford Oxford University Press p 202 206 2 Reinherz EL and Schlossman SF 1980 The differentiation and function of human T lymphocyles Cell 19 821 827 3 Aiuta F Cerottini J C Coombs RRA Cooper M Dickler HB Froland S Fudenberg HH Greaves MF Grey HM Kunkel HG Natvig J Preud homme J L Rabellino E Ritts RE Rowe DS Seligmann M Siegal FP Stjernsward J Terry WD and Wybran J 1975 Identification enumeration and isolation of B and T lymphocyies from human peripheral blood International Union of Immunological Societies IUIS Report July 1974 Clin Immunol and Immunopathol 3 584
18. 1 0 196 x 2 3 4 5 6 1 2 8 2
19. 12 13 NK CD8 NK CD8 CD8 T CD8 NK T8 FITC CD3 T 14 15 16 Aik ABE
20. T U B TdT HLA DR Smlg T B T B T Pan T CD7 CD2 CDS CD3 CD3 CD4 CD8 CD1a CD4 CD8 CD2 CD3 CD5 CD7 CD4 CD8 T B pan B
21. 30 2 250 kL 6 PBS 2mL MA BUE Y 7 400 450 x g 5 8 6 7 9 0 5 1mL PBS 10 EPICS Cytomics 2 Ficoll Paque 1 12mm x 75mm 2 Ficoll Paque 1 x 10 48 4H 1 x 10 mm 1mL 3 2 8 CE 400 450xg 4 4 200L
22. T4 T8 CD4 CD8 MS SLE CD4 T4 T8 SLE CD8 TA T8 H SLE CD4 CD8 SLE CD4 T4 T8 SLE SLE CD4 CD8 SLE T4
23. 6 Ficoll Paque Pharmacia 17 0840 03 EDTA 1 1 100 L 3 10 x 10 mm 10 x 10 mm 3x 109mm PBS 20 25 6 a gt 10 x 10 mm 10 20 x10 2 20 30 x 10 3 30 40 x 10 5 40 60 x 10 6 60 100 x 10 10 100 200 x 10 20 2 6 b 3 x 10 mm 1 25C 500xg 1 700rpm 5 2
24. CD19 B pre pre B CD20 pre B CD19 CD20 B B B Eb 5 B CD21 CD22 B B CD22 pre pre B CD22 T T CD4 T CD8 2 T CD8 CDS T8 CD8 T T8 CD8 T8 DIiGeorge SCID
25. 12 25 000 110 gL 25L 13 Propidium lodide 0 01mg mL 104L 14 30 Acridine orange 0 005mg mL 10L 3 15 16 100 85 gt PBS PBS PBS 2 8 C CRF NBS 2 BSA 0 1 0 1 PBS PBS 0 1 2 8 C
26. 1989 Monoclonal antibodies for diagnosis of immunodeficiencies Blut 59 200 206 Collier AC Meyers JD Corey L Murphy VL Roberts PL and Handsfield HH 1987 Cytomegalovirus infection in homosexual men Relationship to sexual practices antibody to human immunodeficiency virus and cell mediated immunity Am J Med 82 593 601 14 Taylor MGJ Fahey JL Detels R and Giorgi JV 1989 CD4 15 16 17 18 19 20 21 22 23 percentage CD4 number and CD4 CD8 ratio in HIV infection How to choose and how to use J AIDS 2 114 124 Morimoto C Hafler DA Weiner HL Letvin NL Hagan M Daley J and Schlossman SF 1987 Selective loss of the suppressor inducer T cell subset in progressive multiple scferosis Analysis with anti 2H4 monocfonal antibody N Engl J Med 316 67 72 Reinherz EL Weiner HL Hauser SL Cohen JA Distaso JA and Schlossman SF 1980 Loss of suppressor T cells in active multiple sclerosis Analysis with monoclonal antibodies N Engl J Med 303 125 129 Weiner JL Hafler DA Fallis RJ Johnson D Ault KA and Hauser SL 1984 Altered blood T cell subsets in patients with multiple sclerosis J Neuroimmunol 6 115 121 Smolen JS Chused TM Leiserson WM Reeves JP Alling DW and Steinberg AD 1982 Heterogeneity of immunoregulatory T cell subsets in systemic lupus erythematosus Am J Med 727 783 790 Smolen JS Morimoto C Stetnberg AD Wolf A Schlossman
27. SF Steinberg RT Penne E Reinherz EL Rerchlin M and Chused TM 1985 Systemic lupus erythematosus delineation of subpopulations by clinical serologic and T cell maTker analysis Am J Med Sci 289 139 147 Morimoto C Reinherz EL Schlossman SF Shur PH Mills JA and Steinberg SD 1980 Alterations in immunoregulatory T cell subsets in active systemic lupus erythematosus J Clin Invest 66 1171 1174 Raziuddin S Nur MA and Alwabel AA 1989 Selective loss of the CD4 inducers of suppressor T cell subsets 2H4 in active systemic lupus erythematosus J Rheumatol 16 1315 1319 Sato K Miyasaka N Yamaoka K Okuda M Yata J and Nishioka K 1987 Quantitative detect of CD4 2H4 cefls in systemic lupus erythematosus and Sjogren s syndrome Arthritis Rheum 30 1407 1411 Ramos EL Turka LA Leggat JE Wood IG Milford EL and Carpenter CB 1989 Decrease in phenotypically defined T helper inducer celfs T4 4B4 and increase in T suppressor effector calls T8 2H4 in stable renal allograft recipients Transplantation 47 465 471 5 6 24 25 26 27 Pedrazzini AS Freedman AS Andersen J Heftin L Anderson K Takvorian R Canellos GP Whitman J Coral F Ritz J and Nadler LM 1989 Anti B cell monoclonal antibody purged autologous bone marrow transplantation for B cell non Hodgkin s lymphoma Phenotypic reconstitution and B cell function Blood 74 2203 2211 Reinherz EL Kung PC Goldst
28. 597 4 Reinherz EL Haynes BF Nadler LM and Bernstein ID 1986 Leukocyle Typing ll NewYork Springer Verfag Vol2 p 8 15 20 37 5 Reinherz EL Meuer SC and Schlossman SF 1983 The delineation of antigen receptors on human T lymphocyles Immunol Today 4 5 8 10 11 12 13 Reinherz EL Morimoto C Fiizgerald KA Hussey RE Daley JF and Schlossman SF 1982 Heterogeneity of human T4 inducer T cells defined by a monoclonal antibody that delineates two functional subpopulations J Immunol 128 463 468 de Martin RM and Parker JW 1989 Immunologic alterations in Human immunodeficiency virus infection A review J Clin Lab Anal 3 56 70 Morimoto C Letvin NL Distaso JA Aldrich WR and Schfossman SF 1985 The isofatfon and characterization of the human suppressor inducer T cell subset J Immunol 134 1508 1515 Morimoto C Letvin NL Distaso JA Brown HM and Schlossman SF 1986 The cellular basis for the induction of antigen specific T8 suppressor cells Eur J Immunol 16 198 204 Meuer SC Schlossman SF and Reinherz EL 1982 Clonal analysis of human cyiotoxic T lymphocyies T4 and T8 effector T cells recognize products of different major histocompatibility regions Proc Natl Acad Sci USA 79 4395 4399 Reinherz EL Cooper MD and Schfossman SF 1981 Abnormalities of T cell maturation and regulation in human beings with immunodeficiency disorders J Clin Invest 68 699 705 Schmidt RE
29. T8 T4 T8 T4 T8 CD4 CD8 T4 T8 CD4 4 6 T8 CD8 T8 809 T 30 lt 359 NK T8 B CD20 8510096 AMC BRAHMS 3 CV 5
30. ein G and Schlossman SF 1979 A monoclonal antibody with selective reactivity with functionally mature human thymocytes and all peripheral human T cells J Immunol 123 1312 1317 Reinherz EL Haynes BF Nadler LM and Bernstein ID eds Leukocyte Typing Il Human T Lymphocyies 1986 New York Springer Verlag p 8 9 Koepke JA and Landay AL Precision and accuracy of absolute lymphocyie counts Clin Immunol Immunopathol 1989 52 19 27 135 0063 5 7 TOC TEL 0120 566 730 FAX 03 5530 2460 135 0063 5 7 TOC Wa COULTER 6603274_0911 6 6
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