Untitled - 大陽日酸 SI事業部
Contents
1. 1 1 Gehrmann ML Hatout Y and Fenselau C 2004 Evaluation of Metabolic Labeling for Comparative Proteomics in Breast Cancer CellsJournal of Proteome Research 3 1063 1068 2 McGregor E Dunn M J 2006 Proteomics of the heart unraveling disease Circ Res 98 309 21 3 Matt P Carrel T White M Lefkovits l Van Eyk J 2007 Proteomics in cardiovascular surgery The Journal of Thoracic and Cardiovascular Surgery 133 210 214 4 Gygi S P Rist Gerber S Turecek F Gelb M H and Aebersold R 1999 Quantitative analysis of complex protein mixtures using isotope coded affinity ta2. 9 HMS MS
3. MS 1 SI SS A89 0126 SISS 1 80 C CRZVIVUBIDRERIC EU ETOT POETE PESO CEU URS v TIENT TERTE mue SS 5 S5 685 LX1 SSS 725mLx1 mM GSSG 12mLX1 50mM GSH 12mLX TA DNAX1 pUC BAP 50uLX1 10ng mD ORAT 1mLX1 exin 1 1 1
4. atom atom 60 816 5 Glycine C N Ethyl Ester HCI 99 C 60 4704 Llysine 1 PC HCI 99 C 98 N 58 9322 L Lysine 2 CHCI 99 C 2929 4 Glycine N 98 N 64 3459 L Lysine C HCI 9596 CP 98 C 59261 7 Glycine PN d Em 608041 L Lysine C N EE 33 645 9 Glycine 2 2 d 98 D 59 290 0 LLysine 2 N 2HCI 98 CP 98 N 27 943 9 Glydne27C 99 60 896 3 L Lysine 2 N 98 N 299324 Gycne2 CN EE 60 897 1 L Lysine N HCI 98 N 60 902 1 L Lysine N HCI 98 N m x 61 6214 LLysine 3344 55 66 d HCI 98 D 4866 8 BOCGly OHA 99 61 6192 LLysine 445 5 d HCI 98 D 587725 BOCGly OH LCUN 99 642037 LLysine 6 C2HCI 99 98 N 58936 5 L Lysine 6 UC HCI 99 C 604992 BOCGy ORG 99 C 6066 5 Liysine 6 C SN HCI RN OR E 653632 FMOC Lys BOC OH PC N 97 CP E FE 7 EDT cE OT Ret EE 485780 2 60 516 1_ 1 2 a 7N 987N 489557 BOCGIyOH Z CUN 49 007 5 BOC Lys Z OH N 98 N 98 N 49 008 3 L Methionine 1 UC 99 C 605182 EMOCGIy OR T PC 99 C 58977 2 L Methionine 2 C 99 492698 FMOC GIy OHT CN 99 60 527 1 L Methionine C methyl C ENG 99 N 58 982 9 L Methionine 2 C N 99 FC 587
5. MS D C N 2 2 MS J MS
6. 2014 3 SI P2 O Water O PPP eeeeeeeeeeeeeeeeeeseeeseseeeseeeeeseeeeeeeeessesseeeeseeeeee P7 S 4 P9 P13 14 NNP Py Tag 1
7. r A29 0058 Quick 1 80 099 lt Quick wem DNA 1 CAT 800ng mU m F Quick 2 y 73e 1mLX1 50pL X20 Quick DNA 2 pUC CAT 50uLX1 10pg mD 1 2 CAT ESLTriple QMS KN Om s 1 os 1 Em 2 OQ A107014 UL d 1mL A42 0075 UL GN d 1mL Sg o A390072 7 ULN 1mL A91 0128 7 2
8. C N O O SOTEC ISOTEC 1 N D D p X Y 0 H D OH NH2 NR R2 TsO MsO R etc V z 0 R OH o NH2 D D p lp p Xp p Xp D Xp D D D D D D D N N N N N amp R R H Bn Me PhCH CH BOC etc 1 ORR
9. AB Py Tag 50 4 1 2 i 4 5 py0 4 a A 7 Py6 m z 6 a mamam uma ma B Py 12 mamam ama oy C W Py LGM K Py DGYRI Py Tad 3 BOKBIANZAZ i O 2 v Z b ei M E o D 5 31x uoissaJdx3 uia30Jd 07
10. HasO 299 9996 1998 1g 109 20g 50g LL 18 980atom 17 20atom 16 lt 20atom m mg L ICP mgl ICP 01mg ICP mg L ICP mgl ICP 2mg L ICP mg L ICP mgl img mg L mgl mg L mgl 1mg L mgl PH 55 80 pH lt 03ms m TOC lt 5mg L TOCH E mL lt 025EU mL LAL 03 IB 0 H N R2 RENHA HO Emme gt lt Rd A Enzyme Chymotypsin Asp N gs G Mass shit 2 Q dS og HO Enzyme d
11. 3 O T E2 m Z 2 0 gt O 2 4 Ju Mo o O9 sn e 2909 Ben c 5 a V 03 AR gt 95atom 0 QO O A101 0138 A99 0136 O 98atom9 0 NN wO 95atom9 0 fon Maleic pu S 4 Fluorobenzaldehyde to o lt OEC OL T 5 ignard Reaction pe iHd seriei 968 edd A102 0139 O 98atom9 O O Ethyl cinnamate 15O o Y j c 99 0 atom Oo Yield 524 O A100 0137 A106 0143 A104 0141 A105 0142 IUD O Oz 5 O jd s Jy He To o cu w 13
12. BAP 2 511 rre UA een A29 0059 SI 1 80 C 14285 lt SI gt FC E U r CAT 5mo mL SI 1mLX1 1 10mLX1 1 1 1 Quick
13. e lsotec Stable Isotopes 01 cei Biomolecular MS MS Biomolecular MS MS O D C N RR O
14. 200mM 100 L 0 5mL 1 200mM Py6 80uL 05mL X12k 200MM Py12 80L 0 5mL 1 A SmL X1 3 amp B 5mL7 2 7 x 1 Ph x10f8 X10 1 PyrTag 0 Py Tag 6 Py Tag 12 7A CLS 200mM 200mM 200mM 30uL 30uL RBE Py Tag Py0 Py6 Py12 1 6 12 Py Tag Py Tag Dn m 2 Py Tag 0 CH ii Py Tag 6 18147 Py Tag 12 lt HN 187 19 05 W 1 2 3 Py Tag 4 5
15. atom atom9 6 48 93 7 Algal Fatty Acids C 99 59 096 7 99 42 620 2 AlgalLipid Mixture ENS 61 609 5 Octanoic 8 8 8 d3 Acid 99 D 61 349 5 1 Amino octane d Octylamine 98 D 59 085 1 Octanoic 8 C Acid 99 C 58781 8 Decanoic Acid 1 2 C ENE 44821 4 Octanoic d Acid 98 D 48 865 8 Decanoic Acid 1 C Capric Acid 99 c 64 645 8 Oleic Acid 1 2 3 7 8 9 10 C 99 C 61 612 5 Decanoic 10 10 10 d3 Acid 99 D 490423 Oleic Acid 1 C 99 57 966 1 Decanoic 10 CAcid 99 60 577 8 Oleic Acid 1 C Potassium Salt 99 48 866 Decanoic d Acid 98 D 49 043 1 Oleic Acid C18 99 57 253 5 Glyceryl1 2 Dioctadecanoate 3 octanoate 1 C 99 C 64646 6 Oleic Acid 9 10 C EN 60 579 4 Glyceryl 1 3 Dioctadecanoate 99 61 613 3 Oleic 9 10 d Acid 98 D 60 560 3 Glyceryl Trihexadecanoate ENE 66 158 9_Oleic acid 1 C S amp P tested 997 42 5907 Glyceryl Tri hexadecanoate 99 66256 9 Oleic24 68 10 12 14 16 18 C acid 99 61 547 1 Glyceryl Tri hexadecanoate 16 16 16 d3 99 D 48 961 1 Palmitic Acid 1 2 3 4 C 99 61 696 6 Glyceryl Tri hexadecanoate d31 98 D 60 580 8 Palmitic Acid 1 2 3 4 C Potassium Salt 99 49 266 3_ 1 Trstearin 99 48 580 2 Palmitic Acid 1 2 C 99 61 611 7 Glyceryl Tri octadecanoate 18 18 18 d3 99 D 29 212 5 Palmitic Acid 1 C Hexadecanoic Acid EN 42 589 3 Glyceryl Tri octanoate 1 C 98 CP 99 C 48 964 6 Pal
16. ing HO Enzyme Enzyme Trypsin Glu G ysc Mass shit 4 B HO Enzyme Ho D ct Ko D wn h T c e 3 o Casein MS MS MS Mw 1758 9 50 Hz 0 series H lelel dll LLL mon lt yseries MS 17620 618 11 5095150 1760 0 la 2 i gt T 409 soo 7 Roos 2700 660 c900 7 oao 1189 ieo Tao 1400 isoo M00 200 teeo m z AXIMA OIT SHIMADZU BIOTECH RR lt I zu yz sg o TPy Tag TI O 22 ELM LEE 2 ror PROTEINS Py Tag for PROTEINS MS Z 02 A132 0178 Py Tag for PROTEINS 1 10 m
17. Water O 1 O O Water O O O C O O O MS O O O O gt 98atom9 6 99 99 gt UA o E o ct Ke D wn o ct
18. LysArg ULTC PN 1mL o5 A41 0074 UL SN d 1mL A92 0129 lysLeu UL TCTN 1mL 40 0073 7 UL CN 1mL A1080145 7 SeMet mL g oD c 1MS 0 o GO E 53 373557917227111 A SDS PAGE B C ma iD ka unl mel em tamae e mm S MET T 3 om 99 em w 17100 25 mi 20 T Ll Lg w Ca M v HT KRR nmm imasna ORERE SeMet M Kigawa T et al Cell free Protein Synthesis Methods and Protocols a Spirin A S amp Swartz J R eds 83 97 2007 I Matsuda T et al J Biomol NMR 37 3 225 229 2007 MI Yabuki Tet al J Struct Func Genomics 8 4 173 191 2007 lB Seki E et al Anal Biochem 377 156 161 2008 MI Yokoyama Jet al Anal Biochem 411 223 2292011 OZ ICGBRUD NIRE 2 CEURRERRE CT BUS UTRIUS pU 71 VI SSO
19. Xin vivo C N G HER 2 3 c wn c 3 lt c EIS e TAIYO NIPPON SANSO The Gas Professionals lsotec Stable Isotopes SI 7142 8558 1 3 26 88 8550 FAX 03 5788 8710 4 E mail lsotope TNS tn sanso cojp UR RLI ilelsotope in sanso cojp MJ 46 14 03 0 5K TL po
20. 648302 Trp OH N 98 98 48 5829 Tyrosin2 6 d phenyl 2 6 98 FMOC Trp OH N 98 CP TN Tyrosinl 2 6 d phenyl 2 6 d D 60921 8 FMOCTrp OH a FN 98 N 59 1092 BOC Tyr OH N 60483 6 L Tryptophan 1 C 99 c 65362 4 FMOC Tyr OH N 97 CP 98 N 60484 4 L Tryptophan 2 C Indore Ring C 99 C 49016 4 L Valine 1 C 99 B11 0013 L Tryptophan Cn N 97 60 491 7 L Valine 2 C 99 c 95 60 014 8 L Valine C SN 98 C 812 0014 L Tryptophan C Nyd FAQ 98 N 957 49017 2 LValine N 98 N 60 906 4 LTrptophan N a amino N 99 N 48 6027 L Valine d 98 D 805 0007 L Tryptophan N 95 N 60497 6 BOC Vak OH l C 99 B13 0020 L Tryptophan N d 48 601 9 BOC Val OH N 98 N 615862 Lryptophan 2 4 55 7 d Indole dj 97 D REGN TM EN 60 984 6 L Tyrosin 4 hydroxy O 40 0 642886 FMOCVaLOH CN 98 60 991 9 L Tyrosin 4 hydroxy O 95 0 hi 58 784 2 L Tyrosin 122 3 UC Eka 48 600 0FMOC Val OH N 98 N 48 982 4 L Tyrosin 1 C L 4 Hydroxyphenylalanine 9 1 608 7 FMOC Val OH d 98 D atom atom 5876133 D Fructose T 6 C 99 C 60 685 5 D Glucose 75 C 41 555 3 D Fructose 1 FC 99 iC 64 684 9 D Glucose C Endotoxin tested 99 C 41 55 3 D Fructose 1 C 99 c 55215 1 D Glucose C C d ERG 58762 1 D Fructose C 99 97 990 492140 D Fructose 2 C 99 60 823 8 D Glucose C C d An EA EN 3I08T G D Glucose T d 98 D 415545 D Gal
21. Acid 98 D 48 585 3 L Alanine 2 PC FN 99 C 98 N 586722 LAlanine 1 C3 3 3 4 99 18998 0 Aspartic 233 d Acid 98 D 99 D 61 606 0_L Aspartic 2 3 3 d Acid N Acetyl Derivative 98 D 60468 2 L Alanine 2 3 C 99 C 48 997 2 L Aspartic Acid 1 C 99 48 987 5 L Alanine 99 c 58 628 5 L Aspartic Acid 1 C N 99 c 48 988 3 L Alanine 3C SN 98 c 98 N 98 N 60 783 5 L Aspartic Acid C PN 98 3C 49 0822 B Alanine C 5N 99 C 98 N 98 N 58 616 1 L Aspartic Acid 3 4 C 99 c 332127 L Alanine N 98 N 58 879 2 BOC Asp OH N 98 N 60 905 6_B Alanine N 98 58 618 8 BOC Asp OH 3 C 99 48 586 1 L Alanine 2 d 98 D 58 640 4 BOC Asp OH 4 C 99 48 992 1_L Alanine 3 3 3 d 99 D 68 363 9 FMOC Asp OtBu OH C N ENG 48 845 L Alanine 23 3 3 d 98 D 59 407 5 FMOC Asp OtBu OH N 98 N 48 676 0_BOC Ala OH 1 C 99 C 58 862 8 FMOC Asp OH 1 C 99 49 288 4 BOC Ala OH C C depleted 999 C 49 290 6 FMOC Asp OH N 98 N 58 674 9 BOC Ala OH C 99 c 60 263 FMOC Asp OH 4 C 99 48 583 7 BOC Ala OH C N 99 C 60 010 5 LCystine Ns 98 N 98 N 01 0001 L Cystein N HC T 95 5N 48 991 3 BOC Ala OH N 98 404 0022 1 Cystein C Nd DC DO 98 C 60 507 7 BOC Ala OH 2 C 99 c 98 N 0344 9 BOC Ala OH 2 7C N 99 C 98 D 98 N 02 0002 L Cystein C N HC H O 98PC 48 678 7 BOC Ala OH 3 3 3 d 99 D 98 N 492892 BOC Ala OH 3 9C 99 C 03 0021 L Cystein N d 0 0 CS m 98 66054 FMOCAlr OH GN FN 67 660 8 FMOC Cy
22. 010 5 L Phenylalanine N 98 60 806 8 L Leucine C N 95wt96 chiral purity FE 61 889 L Phenylalanine 3 3 d 98 D 58943 8 L Phenylalanine 2 d 98 D 59 627 2 Lleucine C N 99wt chiral purity FP 192957 Pe 99 C 07950 Lisudne Cd 95 C 48 596 9 BOC Phe OH 1 C 99 c 98 D 48 883 3 BOC Phe OH N 98 N 34 096 0 L Leucine N 98 N 60 520 4 BOC Phe OH 2 C 99 C 48 681 7 L Leucine 2 99 C 49 297 3 BOC Phe OH 3 C ENS 60 765 7 L Leucine 2 CN 99 C 58 955 1 BOC Phe OH phenyl d 98 D lt 98 PN 48 597 7 BOC Phe OH phenyl d 2 3 3 d 98 D 60482 8 LLeucine 3 C 99TC 651443 FMOC Phe OH C SN 98 60 817 3 LLeudine 3 TCFN 99 C 98 5N 98 N 60907 2 FMOC Phe OH N 98 N 61 607 9 L Leucine 3 d 99 D 49 296 5 FMOC Phe OH 2 C 99 c 61 597 8 L Leucine 4 d 99 D 61 599 4 FMOC Phe OH phenyl d 2 3 3 d 98 D 48 682 5 L Leucine 5 5 5 d 99 D 58 949 7 L Proline 1 C 99 c 49 294 9 L Leucine d 98 D 80 8114 L Proline C SN 98 C 61 598 6 L Leucine d isopropyl d 98 D 98 N 48 594 2 BOC Leu OH 1 C H O 99 C 60 899 8 L Proline N 9896 98 49293 0 BOC Leu OH N H O 98 65145 1 FMOC Pro OH C N 98 C 58923 3 BOC Leu OH 2 C N H O 99 c 98 N 98 N 58 951 9 FMOC Pro OH N 98 61 590 0 BOC Leu OH 5 5 5 d H O 99 D 60 914 5 FMOC Ser tBu OH N 98 N 48 593 4 FMOC Leu OH 1 C 99 C 58 960 8 L Serine 1 2 C 99 c 59353 2 FMOC Leu OH C SN 98 49 015 6 L Serine 1 5C 99 9 60471 2 LSerine 2 C ENE 48 595
23. 59 107 6 Acid 99 60 566 2 Valeric 5 C Acid 98 99 60 567 0 6 7 8 C Acid 99 c 4920 1 Valeric d Acid 98 D 59 193 9 Octanoic 7 8 C Acid 99 c atom9 Acetoacetyl PC coenzyme A lithium salt hydrate 95 CP 99 Acetyl 1 2 C coenzyme A lithium salt 95 CP 99 C 65 575 9 Malonyl C coenzyme A lithium salt 95 CP 99 C 70 388 5 Octanoy 24 68 C Coenzyme A lithium salt 95 CP 99 PC 67 576 8 Oleoyl C1 coenzyme A lithium salt 95 CP 99 C 65 820 0 Palmitoyl 1 C coenzyme A lithium salt 9596 99 C 65571 6 Palmitoyl C coenzyme A lithium salt 95 99 C 67 577 6 Stearoy C coenzyme A lithium salt 95 99 G 1 r 85 2 1 5 MS chromatogram O JEEP mass spectrogram e gt A 9 D yw dic 3 2
24. L Asparagine C H O 99 C 802 0004 L Glutamine N 95 EN 64 195 2 L Asparagine C N 95 CP FS 616303 LGlutamine 23 3 44 d 98 D 63 6592 L Asparagine C Nyd 98 TC 806 0008 L Glutamine Cs Nz 2d 570737 L Glutamine Ny dio MN L Asparagine C NH 5596 P ER 63 508 1 LGlutamine C N di ENE 58869 5 Asparagine 99 C IN 48 589 6 L Asparagine N H O amide N 98 N 08122 LGlutamine CN amine N 99 C 48 996 4 L Asparagine N H O amine N 98 N 98 N 64 196 0 L Asparagine N 9896 CP 98 N 60 915 3 FMOC Glu OtBu OH N 98 N 63 667 3 L Asparagine N d 9896 98 N 58 840 7 BOC Glu OBzl C N 9796 CP 98 C 98 D 58768 0 BOC Glu OH 1 C 99 C 57 074 5 L Asparagine N d D O 98 CP 28 0 49 000 8 FMOC Glu OHTN 98 N 18591 8 L Asparagine N HO 9896 CP 98 N 557995 a ER N E 66 875 3 FMOC Asn Trt OH UC N 99 C Parm 98 N 299340 Glycine 1 C N 997C 6874 5 FMOC Asn Trt OH N 98 N E 98 N 57 989 0 FMOC Asn OH N 98 N 608076 Glycine 1 7622 d ag 60 913 7 FMOC Asn OH a amine N 98 N TIC RN CUN E 57 9785 BOC Asn OH a N amine NJ 98 N 60677 4 Glydne C N G N depleted 2224 60 485 2 L Aspartic Acid C 99 28382 7 Glycine C 99 57 979 3 L Aspartic Acid 1 99 c 48 952 2 Glycine C SN 98 C 60 489 5 99 98 61 753 9 99 c 09
25. 0 FMOC Leu OH N 98 5N 604720 L Serine 3 C 99 C 61 594 3 FMOC Leu OH 5 5 5 d 99 D 48598 5 L Serine 2 CN 99 59 040 1 FMOC Leu OH di 98 D 98 N ES c 9 e E e 9 Q e c 11 7 E atomi atom 60488 7 L Serine C 99 c 60510 7 LTyrosin 2 C 99 09 0011 L Serine C N 98 48 985 9 L Tyrosin 3 C 99 c Ui Lise NUET EE 60 509 3 L Tyrosin 4 C phenyl 4 C 99 c gt 90503 4 L Threonine 1 FC 99 C 189794 L Tyrosin C phenyl CJ 9 B10 0012 L Threonine C N 97 C 49 286 8 L Tyrosin C 98 C 957N 6099 1 L Tyrosin C SN 98 C 60 777 0 L Threonine C N 98 7 98 N 04 0006 L Threonine N 98 N 33215 1 L Tyrosin N 98 N 67 286 6 BOC Thr Bzl OH C SN 99 c 48 984 0 L Tyrosin 3 3 d phenyl 3 3 d 98 D 69427 4 FMOC Thr tBu OH C N 99 c 48 981 6 L Tyrosin 3 5 d phenyl 3 5 d 98 D 67 697 7 FMOC Trp Boc OH N 97 N 48 980 8 L Tyrosin d phenyl d 98 D
26. 74 5 FMOC Gly OH C 99 98 N 48953 0 FMOC Gly OH C N Eis 65 1400 L Methionine C d methyl CH d F 283756 EMOCGN OR N BN 299154 L Methionine Cd methyl Cd Ag A FO 608149 L Methionine T Cd methyd Ag 60345 7 FMOC Gly OH 2 C N 99 TC q Lon y EET 60810 6 L Methionine C SN EN 807 0009 L Histidine C N XX 60 924 2 L Methionine N 98 CP 98 N s 58 983 7 L Methionine PN N Acetyl Derivative 98 N 60 808 4 L Histidine UC 2 N a amine N 58 9802 L Methionine 2 d 98 D 5050005 Hinc N EM 30061 6 1 Methionine d methyl 98 D 60 922 6 L Histidine 2 N a amine N 589845 BOC Met OH 1 C 99 C 676969 FMOCHIs TR OR N 98 N 58 985 3 BOC Met OH C methyl C 99 60477 1 L lsoleucine 1 PC 99 C 60 511 5 FMOC Met OH 1 C 99 60 809 2 L Isoleucine C SN 98 C 65 364 0 FMOC Met OH C N 97 CP wE 98 N 60 809 2 L soleucine C N 9596 CP 97 7C 50979 6 FMOC Met OH TN 98TN 95 60 504 2 L Phenyl 1 C alanine 99 c 60 901 3 Llsoleucine 9896 CP 98 5N 60 487 9 L Phenyl Ccalanine 99 C 59 722 8 FMOC Ile OH C 99 C 61 587 0 L Phenyl d alanine 98 D 98 N 490148 L Phenyl d alanine 2 3 3 d 98 D 57 862 2 FMOC Ile OH N 98 N 4909 1 L Phenylalanine 1 C 99 C 60 490 9 L Leucine 1 2 C 99 C 490113 LPhenylalanine 2 C Eha 49 005 9 Lleucnel7C 39 C 49 012 1 Lhenylalanine 3 C 99 49 006 7 ELeucneTRCTN P 0 8017 L Phenylalanine C SN 98 C S 387N 60 523 9 L Leucine C 99 49
27. ERE C EENET RERE 08 ES e 9 e r atom atom 49287 6 L Alanine C 99 9 C 48 999 9 L Aspartic 4 C Acid 99 48 986 7 L Alanine 1 C 99 60 770 3 L Aspartic 2 C N Acid 99 c 48677 9 Alanine 2 99 C 98 N 483948 LAlanine 3 C 99 33213 5 L Aspartic FN Acid 98 N 60 8025 L Alanine T CN 99 60 910 2 L Aspartic N Acid B Benzylester Derivative 98 N 98 N 572518 L Aspartic N 2 3 3 d
28. P tested 99 66 067 1 Lauricacid 1 C S amp P tested ENS 65 414 0 Potassium palmitate 16 16 16 d3 EN 60 573 5 LinoleicAcid C18 59 49 041 5 Sodium Octanoate 1 C Octanoic Acid Sodium 99 C 60 581 6 Linoleic Acid C18 Potassium Salt 99 Salt 605743 linolenic Acid C18 99 C 57 700 6 Sodium Palmitate 244 6 8 10 12 14 16 C8 99 49 086 5 Myristic Acid 1 2 C 99 C 65 720 4 Sodium octanoate 2 4 6 8 C 99 c 29 087 3 Myristic Acid 1 C Tetradecanoic Acid ENS 59 160 2_Stearic Acid 1 2 C 997C 05689 Myristic Acid C14 99 C 299162 Stearic Acid 1 C Octadecanoic Acid ENa 1 416 5 Myristic 13 13 14 14 14 d Acid 98 D 60 558 1 Stearic Acid C18 99 C 589756 Myristicz14 14 14 d3 Acid 99 D 61 5846 Stearic 17 17 18 18 18 d Acid 98 D 389748 Myristic14 C Acid 98 C 49 0393 Stearic 18 18 18 d3 Acid 98 D 36 688 9 Myristic d Acid 98 D 60 565 4 Stearic 18 C Acid ENa 68 115 5 Myristic acid 1 C 5 amp P tested EN 49 315 5_Stearic 2 2 d Acid 98 D 493163 Octanoic cid 12 34 C 99 59 149 1 Stearic 2 C Acid 99 592056 OcanolcAcid 12C 99 Pe 44 824 9 Stearic d35 Acid 98 D 0 583 2 Octanoic Acid 1 C Extra 997 59 138 6 Tridecanoic 2 2 d Acid 98 D 605840 Octanoic Acid 1 C OBT Grade 99 C 60 561 1 Undecanoic Acid 1 C 99 c 29 645 7 Octanoic Acid 1 C Research Grade Caprylic 99 C 63712 2 Valeric Acid 34 5 C 99 C Acid 59 644 2 Valeric acid 1 C EN 605727 Octanoic Acid CE 9 60 582 4 Valeric 2 C Acid Pentanoic Acid ENE
29. actose I T 60 550 6 D Glucose2 5 Cs C 0537 9 D Galactose C 98 C 310794 SELL 99 49 507 7 D Galactose 1 d 98 D EE 9 30606 1 GiycsroHOD 3 98 D 61549 8 D Glucose 3 d 98 D 454524 Glycerol 1 1 2 3 3 d 98 D 609468 D Glucose 45 454524 Glycerol 1 1 23 3 ds 98 D rm 492639 Glycerol 13 39 C 28 265 0 0 61 6 6 1 98 59 7163 D Glucose 6 6 d for clinical investigational use 98 D 48947 6 Glycerol C 99 c x 485484 GiyceraL21 oc 397 31 080 8 D Glucose 6 C 99TC 4449 8 Glycerol d 98 CP 98 D RAT EETI 44749 8 Glycerol d 9896 CP 98 D 605328 Diactose 1 C 39 48 873 9 D Glucosamine 1 C HCT 99 c 35837 39 6021 1 D Glucosamine 1 C N HCI 592994 98 C 60 928 5 D Glucosamine N HCT 98 D 60 5344 ENS 45318 8 D Glucose 12 99 60538 7 D Manaose 6 4531956 DLGlucose16C 98 61 620 6 D Sorbitol 1 16 6 d 98 D 29704 6 D Glucose 1 C 99 C 189782 D Sorbitol 1 UC D Glucito 99 49 216 7 D Glucose C C depleted Dextrose 993 C 60 551 4 D Sorbitol in 90 C 389374 D Glucose C 99 605522 Dsorbitel2 99 60 6847 D Glucose C 30 C 33 104 DXylose 1 C ENE ES c 9 e Vis Eae
30. gs Nature Biotechnology 17 994 999 5 Ong SEE Blagoev B Kratchmarova l Kristensen D B Steen H Pandey A and Mann M 2002 Stable Isotope Labeling by Amino Acids in Cell Culture SILAC as a Simple and Accurate Approach to Expression Proteomics Molecular and Cellular Proteomics 1 376 386 6 Mirgorodskaya OA Kozmin YP Titov MI K rner R S nksen CP Roepstorff P Quantitation of peptides and proteins by matrix assisted laser desorption ionization mass spectrometry using O labeled internal standards Rapid Commun Mass Spectrom 14 1226 1232 2000 7 Yao X Freas A Ramirez J Demirev P A amp Fenselau C 2001 Proteolytic180 labeling for comparative proteomics model studies with two serotypes of adenovirus Anal Chem 73 2836 2842 8 Fenselau C 2007 A review of quantitative methods for proteomic studies Journal of Chrornatography B 855 14 20 BK Overview of Stable Isotope Usage in Protein Labeling 9 B A on 10 e Light Heavy Grow Cells containing Grow Cells containing z proteins of interest proteins of interest Metabolic Labeling Extract proteins Extract proteins Digest proteins Digest proteins e Enzymatic Labeling x P Chemical Labeling Combine Absolute Quantitation Analyze Proteins MS or HPLC MS 02 I
31. mitic Acid 1 C Potassium Salt Potassium 99 C Trioctanoin Palmitate Potassium Hexadecanoate 61712 1 Glyceryl Tri octanoate d 98 D 60 557 3 Palmitic Acid C16 99 646253 1 2 3 7 8 910 7 997 60575 1 Palmitic Acid C16 Potassium Salt 997 48951 4 Glyceryl Tri oleate 1 C Triolein 99 61 610 9 Palmitic 15 15 16 16 16 d Acid 98 D 64624 5 Glyceryl Tri oleate 9 10 C ENS 58 776 1 Palmitic 15 16 C Acid 99 C 60 563 8 Glyceryl C Trioleate 99 C 61 595 1 Palmitic 16 16 16 d3 Acid 99 D 49 267 1 Glyceryl 2 C Trihexadecanoate 99 60 564 6 Palmitic 16 C Acid 99 60 592 1 Guaifenesin C Glyceryl C 99 48 966 2 Palmitic 2 2 d Acid 98 D 1 550 1 Heptanedioic d Acid Pimelic Acid 98 D 48967 0 Palmitic 2 2 d Acid Potassium Salt 98 D 586382 Lauric Acid 1 12 C 99 c 60 5786 Palmitic 2 4 6 8 10 12 14 16 C8 Acid ENE 58 615 3 23 4 99 C 49 2752 Palmitic 2 C Acid 99 58 605 6 276 99 60 570 0 Palmitic 5 6 7 8 C Acid ENa 29216 8 Dodecanoic Acid 99 36 689 7 Palmitic d31 Acid 98 D 48 560 8 Lauric 12 12 12 d3 Acid 98 D 61 437 8 Palmitic d31 Acid Potassium Salt 98 D 48663 9 Lauric 12 PCAcid 99 6659 7 Palmitic acid 1 C S amp P tested 99 48 916 6 Lauric 2 2 d Acid 98 D 66 091 4 Palmitic acid 2 2 d S amp P tested 98 C 57 9688 Lauric 2 C Acid 99 c 66074 4 Palmitic acid d S amp P tested ENG 45 140 1 Lauric d Acid 98 D 66 148 1 Potassium palmitate 1 C S amp
32. s TrtOH PN 98 N 486752 FMOCAla OH 1 C 99 60 496 8 L Glutamic Acid 1 C 99 60 513 1_ 99 C 60 486 0 L Glutamic Acid C 99 48 990 5 FMOC Ala OH PN 98 N 60 785 1 L Glutamic Acid C N ES 61 6044 FMOC Ala OH 2 3 3 3 d 98 D Ed 98 605158 397 644560 LGlutamic Acid d Ag FMOCAROH335d 99 64387 4 LGlutamic Acid Nd 98 N 48 995 6 FMOC Ala OH 3 UC 99 C 98 D 42 619 9 Algal Amino Acid Mixture C 99 c 332143 L Glutamic N Acid 98 N 487910 Algal Amino Acid Mixture C N 98 C 61 628 1 LGlutamic2 3 3 4 4 d Acid 98 D i 99 N 60 5123 LGlutamic 2 C Acid 99 C 60764 9 Algal Amino Acid Mixture C N d ag 490016 L Glutamic 3 C Acid 99 608947 AlgalAmino Acid Mixture N 98 N SAR EAE c 596906 AIOalAmno Acid Mixture Nd 97 D 20251 s C Aud 90 C 98 N 58 770 2 BOC Gln OH N 98 N 633449 L Arginine C HC 9596 CP 98 C 60 522 0 L Glutamine 1 2 99 C 60 803 3 L Arginine 3C N HCI 97 L 95 CP 98 C 60 501 8 1 1 99 C 98 N 60 508 5 LGlutamine 2 99 60 908 0 L Arginine N guanidineimino PN 98 N 60 516 6 L Glutamine C 9 01 0003 L Arginine N 97 CP 95 UN 60 494 1 L Glutamine 3 C 99 C 65 312 8 L Arginine N 9896 L 98 96 N 60 469 0 L Glutamine 5 C amide C 99 65 365 9 FMOC Arg Pbf OH C N 97 CP 98 49 002 4 L Glutamine N amide N 98 N N 48 680 9 L Glutamine N amine N 98 N 579866
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