EZfaast(イージーファースト) (P.56-60)
Contents
1. CU gt 19 MSDS Em 0 6mL CD a 384 1 5mL 6 1 2 2mL 2 3 MS MS P L i C 0 6mL SPE 1 1 2 D 3 3 EN 1 5mL SPE a _ 6 EZ faast SPE 384 EZ faast EZ faast LC MS AAA MS 4u 250X 0mm KHO 7337 308 700 LC MS2. SD1 2 3 2mL Vial ALO 7500 LC MS SD1 2 3 2mL Vial ALO 7501 Ez faast 0 6mL AS0 8534 Ez faast 1 5mL AS0 8535 Ez faast 092085 Ez faast Agilent 092003 GLC Shimadzu GLC Ltd
3. AAA MS 4u 250X3 0mm KHO 7338 308 700 LC MS AAA MS 4u 250x2 0mm KHO 7339 324 900 LC MS AAA MS 4u 250X3 0mm KHO 7340 4 900 GC FID ZB AAA 10mX0 25mml D KGO 7165 279 500 GC MS ZB AAA 10mX0 25mml D KGO 7166 295 700 GC FID ZB AAA 10mX0 25mml D KGO 7167 295 700 GC MS ZB AAA 10mx0 25mml D KGO 7168 311 900 P N EZ faast GC GC MS ZB AAA 10mX0 25mm I D CGO 7169 EZ faast LC MS AAA MS 4u 250X 0mm 00G 4408 BO EZ faast LC MS AAA MS 4u 250X3 0mm 00G 4402 Y0 GC SD1 2 3 mL Vial AGO 7184 GC SD1 2 3 2mL Vial AGO0 63 LC MS
4. x10 000 000 IA Carnosine 441 20 gt 284 00 1 25 500 Pn s 394 pmolmL 5000 1 00 2500 0 75 1 9 2 50 2 3 00 0 50 a 5 75 E a a Aminoadipic acid 02 40000 332 20 gt 244 00 30000 725 pmolmL 0 00 1 0 1 5 2 0 2 5 3 0 3 5 4 0 4 5 min 10000 0 GLC Shimadzu GLC Ltd EZfaast Phenomenex HPLC 01 EZ faast GC MS Model GCMS QP2010 Column ZB AAA 10M 0 25mml D GC Inj Temp 280C Column T
5. Split 1 15 3 Sw 8 5 o 8 8 a MS a E i pe 8 SG 6 TT Interface Temp 280 0 ES A OE 0 9 0 OE i i OC lon Source Temp 200 C 2 3 4 5 6 7 8 Scan range m z 45 450 TIC A0002 i g 8 cc E o Phe 90 4 nmol ml DD 8 8 Ww 8 ue 8 8 50 Se gE E 5 1 gt 3 sl amp a 8 3 S E i V IN 2 3 4 5 HP https solutions shimadzu co jp glc 0Q1 HPLC EZfaast Phenomenex HPLC Column EZ faast V 1 nmol mL GC FID 20 100L Mi 1596 lt 109 RSD
6. 1 1 27278 EZ faast Ez faast Phenomenex EZ faast GC FID LC MS MS STEP 1 STEP 2 SPE STEP 3 SPE NE STEP 4 STEP 5 2 5 STEP 6 STEP 7 _ STEP 8 0 EZ faast https solutions shimadzu co jp glc yo 01 HPLC EZfaast Phenomenex EE 74 74 2 4 2 Nexera LCMS 8030 Column Phenomenex Kinetex C18 2 1x150 mm 1 7 um Mobile Phase A 5 mmol L Ammonium Formate Water B 5 mm
7. 60 6 nn s0 T 3 E 40 1 0 aa i 9 2 cr a i 8 20 ol o 1119 1 UN amp 10 w O amp 0 V J VT 0 EZ faast x1 000 000 3 S eache00nmol ml by GC MS TIC 2 SS 8 8 8 o 8 8 ss gg Sa 8 1 E 8 So SF gt J 2 So 8 Nt 3 SsS 6 8 EE 7 5 8 5 8 7 aw QO 3 2 3 8 3 8S a cS 8 amp SE E 8 8 6 8 8 lo 5 5 e lt 8 amp Cm Sp gt A i 8 8 8 a UU MM UV min 1 0 1 5 2 0 2 5 3 0 3 5 40 4 5 5 0 6 6 0 0 GLC Shimadzu GLC Ltd 4 EE
8. 01 HPLC EZfaast Phenomenex HPLC Column 1 5 L C HPLC A EZ faast EZ faast 7 GC FID MS 7 LC MS MS pmol mL HPLC H P L mV 38 C 80 A 70 gt a 8 8
9. emp 110 C 0min 30 min gt 320 C 0min Carrier GaS He Pressure 15kPa SDIit Ratio 1 15 H P MS Interface Temp 280 C lon Source Temp 200C Scan Range m z 45 450 SCgn Interval 0 15sec x1 000 000 2 S each200nmol ml TIC 8 Eg 8 Eg a 6 i 3 3 8 8 A S oa 8 lt E SE eS E amp 5 SQ So 8 7 5 8 8 3 8 7 b 3 A 8 5 sg ll am Eg sO 2 8 5 8 5 0 6 lg amp E E el 8 RE 3 9 8 ep gt A QA 8 3 8 0 LJLJUU J LL 1 0 1 5 2 0 2 5 3 0 3 5 4 0 4 5 5 0 8 6 0 PKU 100 Instrument GC MSD Phe 1768 6 nmol ml colum ZB AAA 10m x 0 25mmlD E EZ faast BD GC 5 Injection volume 1ul 5 5 8 Injection Temp 250 50 Column Temp 110 C 3min 30 C min 8 r 320 amp ow EES Ps Carrier Gas He g 3 eg ES 8 8 5E
10. ol L Ammonium Formate Methanol Gradient Prog B conc 50 0 min gt 65 1 3 min gt 90 4 4 5 min gt 50 4 51 dS 7 min _ Flow rate 0 4 mL min AL Column Temp 50C Inj Vol 2 uh 1 Kinetex EZ faast 250mL 4um 20min dt 2v 36 STD x1 000 000 200 ESI Positive MRM 1 75 1 50 o 25 4 1 o EE 0 75 1 HPLC 1710 0 50 EE NN 0 0 00 2 T T T T T T T T T T T T T T T T T T T T T T T T T T T T T T 1 0 1 5 2 0 5 3 0 3 5 4 0 min Proly Hydroxyproline PER 52pmolmL
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