JP 2011-217730 A 2011.11.4 10 (57)【要約】 【課題】分泌発現において
Contents
1. O IU UI UI lO 10000 O UU2. 0 I0000B OO 1800000 Mn 100000 E FECE Be SEN B 180000 OO 0 ooo000u4 180000 5 008 00 T J E H H B E x ee u Papa 8 S ES a n i _ C B B 7 _ E B SS B _ O B i SE _ B _ T E E H O H gg x H H B E 008 0 10 20 30 40 50 17 JP 2011 217730 A 2011 11 4
3. la o lOO UU i Ig Se B El E CE 3 de a m r1 a m 1 i aes un C H a _ o i ga Hi 10 20 30 40 50 18 JP 2011 217730 A 2011 11 4
4. JP 2011 217730 A 2011 11 4 6 10 20 30 40 50 7 JP 2011 217730 A 2011 11 4
5. H IO O Hu G l O Od Od Od O O Od Od Od Od O Od a a a T E 2 T Fa Ree E Tos eh C ES SE T S SE EE mM TT h TI AnnAny TT Pom a fl F1 Mia Ti TS Pl m TI 2 E 2 e E n ae Els Ge a E HEN ue PS RS 2 e ES Z 0 ERE a EA SE ES ecn ESCH D E See E Y E e aa a P SS 000000 EA e a 5 bere nnm E E i IO O O SEQ ID NO 1 Primer tetl to amplify the tet gene SEQ ID NO 2 Primer tet2 to amplify the tet gene SEQ ID NO 3 Synthetic DNA encoding the tet gene SEQ ID NO 4 Primer veg3 to amplify the veg promoter SEQ ID NO Priner veg lac4 to anplify the veg promoter and the lacl gene 10 20 30 40 SEQ I D NO 6 SEQ I D NO 7 SEQ I D NO 8 SEQ I D NO 9 SEQ ID NO 10 SEQ I D NO 11 SEQ I D NO 12 SEQ I D NO 13 SEQ I D NO 14 SEQ I D
6. o o o o o ci A m t 3 x EN 3 O L3 O CE o 3 O EE 3 O o 3 You L3 O o I O o O o p JP 2011 217730 A 2011 11 4 12 10 20 30 40 n 50 DU D o JU n
7. C JP 2011 217730 A 2011 11 4 13 10 C UU UU C UU IO UU C n C 20 30 40 UU UU UU JU 50 UU UU
8. C 14 JP 2011 217730 A 2011 11 4 H E C DC non r1 r1 non x H aoe 9 nn EN E EN EN l i s d e E 93 C E d DEE Be li B C C s a et E C C C x n a E 8 u E B B
9. UU o 40 UU 50 JP 2011 217730 A 2011 11 4 5 10 20 30 UU UU o UU UU UU UU UU 40 50 JO
10. UU JU 40 UI 50 JP 2011 217730 A 2011 11 4 11
11. 10 20 30 40 50 JP 2011 217730 A 2011 11 4 15 UU UU JU B JU 10 20 30 C 40 50 C C C C C C C m C C C C C C C 16 JP 2011 217730 A 2011 11 4
12. 2011 217730 A 2011 11 4 3 BU D BU I DU o 10 20 o o 30 40 UU 50 JP 2011 217730 A 2011 11 4 4 Ja UU UU UU UU UU 10 UU UU UU 20 30
13. A 2011 11 4 9 IUD JO a Ja 10 20 30 40 UU UU UU UU 50 UU JP 2011 217730 A 2011 11 4 10 10 20 30
14. G24
15. o 10 20 30 40 50 JP 2011 217730 A 2011 11 4 8 10 20 30 C 40 50 UU UU UU UU UU UU C C C JP 2011 217730
16. 000000000000000000000000000000000 000000000000000000000000000000 10 JP 2011 217730 A 2011 11 4 2 UU UU UU UU UU o 10 20 30 UU UU UU UU 40 UU UU ID 50 UD JU JP
17. JP 2011 217730 A 2011 11 4 19 BESSE JP 0242 Bd Fe PT A3 FRA 11 Sgt B BU B E 355882011 217730 P2011 217730A 43 23 11 4 2011 11 4 51 Int Cl FI Gei E 5 C12P 21 02 2006 01 C12P 21 02 ZNAC 4B024 C12N 15 09 2006 01 C12N 15 00 A 4BO64 C 8 OL 21 R 21 HERE E 2010 254382 P2010 254382 71 HERA 302019245 22 H B H 3 2225118 158 2010 11 15 ECKE Ee 31 AERA 18 8 2009 260998 P2009 260998 BERAEMAB T784815 32 21 11 16 2009 11 16 72 ZAE 33 JP SS L KiETHARH 8 4 61 25 31 8518 38288 42010 71233 P2010 71233 32 22 3 26 2010 3 26 CARME EH 33 RARER AAE OP 26 CAE 8 48 19 275 WEEK 2 8S EE ERT GC SE 8 48 19 217 I AREE 72 SBA RE GOBOGRAGEmGE IBASIBS 247 NA ARABIA TREES CBE 54 RAOR 27 37 808087515 570000 00000000000000000000000000000000000000 000000000000 D0000000000000000000000000000000000000000 D0000000000000000000000000000000000000000 D0000000000000000000000000000000000000000 00000000000000000000000000000000000000 00000
18. NO 15 SEQ I D NO 16 SEQ I D NO 17 19 JP 2011 217730 A 2011 11 4 Primer lac5 to anplify the lacl gene Primer ac6 to anplify the lacl gene Primer glac7 to anplify the glac c promoter Primer glac8 to anplify the glac c promoter Primer glac9 to anplify the gl ac c promoter Primer glac10 to amplify the glac c promoter Primer 3211 to amplify the glac c promoter Primer 3212 to amplify the glac c promoter Syntheti c DNA encodi ng the gl ac c promoter Primer veg13 for PCR 10 Pri mer vegl4 for PCR Syntheti c DNA encoding the AprE signal peptide nulti cl oni ng s ite and pCOLD terni nator SEQ I D NQ 18 SEQ I D NQ 19 SEQ I D NO 20 1 a e Synthetic DNA encodi ng the beta lactamase Syntheti c DNA encodi ng the modi f i ed human interferon a2b Syntheti c DNA encodi ng the modified li pA signal peptide 6 3 9 UD C F tS Gu lt EnvZB RIK1285 Th EY PARE move B lactamase 20 JP 2011 217730 A 2011 11 4 app 2011217730000001 21 JP 2011 217730 A 2011 11 4 D00000000000000 48024 420 CAD1 CAD9 CAI1 G0 DOL DW DO DW 2 FA02 GM1 Hl MA1 48064 AO O02 GOS Go AI CA9
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