IO テスト FITC 標識抗体
Contents
1. IO CD20 Nalm 6 1 Raji 2 95 CD20 JURKAT 3 0 5 1 Nalm 6 pre B ALL 2 Raji Burkitt lymphoma 3 JURKAT T cell Leukemia PBS 4 CD20 Nalma 62. FS SS DU 2 29 EPICS XL E VS FITC Lumphocutes Leukocytes 48 60 80 Count 20 i108 1000 1 1 1 1 1 1 FITC FITC 1 1 HH CYTO TROL 6604248
3. 1 12mm x 75mm 2 Ficoll Paque 5 x 10 5x10 mm 100L 3 20 uxL lOTest IgG1 FITC 20 guL 4 2 8 30 5 PBS 2mL 2 8C 400 x g 5 6 5 7 0 5 1mL 0 59 2 PBS 8 24
4. 5 6 7 8 IO FITC CD20 IOB20a A07772 100 2mL 1 Reinherz EL et al ed 1986 Leukocyte a Typing I Vol 1 SDrinder Verlad pp 303 313 2 McMichael AJ ed 1987 Leukocyte a Typing LI Oxford University Press pp 144 147 210 214 234 238 239 241 480 484 579 581 889 895 3 Knapp W et al ed 1989 Leukocyte a Typing IV Oxford University Press pp 36 38 274 277 295 342 343 4 Schlossman SF et al ed 1995 Leukocyte a Typing V Oxford University Press pp 507 509 515 518 790 794 795 798 1503 1504 5 Beverly PCL and Callard RE 1981 Eur J Immunol 11 105 0001
5. PBS 2mL 2 3 Ficoll Paque 1 PBS lSFicoll Paque 4mL 4mL 1 30 S 2 8 400 xg 8 6 PBS 7 2 lt 8 400 x g 4 8 PBS 9 2 8 400 x g 3 10 PBS 5x103 mm3 5 x 106 mL 11 90
6. i 10 11 S S 6603369 1 500mL pH 7 2 0 2 IO FITC FITC Fluoresc cyanate 100L CD20 35 37kD CD20 pan B CD19 BB scein isothiocyanate 485 509nm 504 541nm
7. 3 5 1 Immunotech a Beckman Coulter Company 130 avenue de Lattre de Tassigny B P 177 13276 Marseille Cedex 9 France 105 0001 3 5 1
8. 4 LH700 5 PBS 5 10 x103 mm 1 12mm x 75mm 2 100L 3 20wL IOTest IgG1 FITC 20L 4 15 5 6 7 400 x g 3 8 7 8 9 0 5 1mL 0 19 0 5 PBS 10
9. 3 6 4 5 6 7 Ficoll Paque 8 9
10. Fc IOTest CD45 FITC CD14 PE A07738 I IM1201 Fc IOTest T B
11. A11895 I A11894 OptiLyse C A07799 I IM3514 IOTest 3 dn dh am 4 IOTest lgG2a FITC A12689 A12690 100 2mL 5 Ficoll Paque Phamacia 17 0840 03 2 1 100 L 5x 103 mm3 10x103 mm PBS 3x103 mm3 1 25 500 x g 5 2 3
12. CD20 CD20 FITC BSA 0 59 0 1 2 PBS VersaLyse PBS 1mL IOTest 3 A07800 IH IM3515 12 5 L 0 19 PBS VersaLyse PBS 1mL IOTest 3 A07800 I IM3515 67 5L 0 59 PBS 3 A09777 I IM3648 VersaLyse IM1400 OptiLyse B
13. Raji 3 10 3 5 5 1 0 19 lt 2 3 329 334 6 Bryan CF Newman JT et al 4340 4344 7 Clark P Normansell DE et al 1987 Blood 67 1600 1606 8 Clevers H Dunlap S and Terhost C 1988 Eur J Immunol 18 705 710 Denning SM et al 1988 J Immunol 141 2980 2985 Freedman AS and Nadler LM 1987 Semin Oncol 14 193 212 11 Foon KA and
14. 2006 3 2004 10 20200EZY00112000 A07772 B IO FITC CD20 IOB20a 1 2 3 4 IO FITC CD20 IOB20a FITC IgG 20 uxL 100 gmL 1 1 IO 2 EDTA
15. Todd RF 1986 Blood 68 1 31 12 Hannet l et al 1992 Immunology Today 13 215 218 13 Haziot A et al 1993 J Immunol 150 5556 5565 14 Keizer G et al 1985 Eur J Immunol 15 1142 15 Kishimoto TK et al 1990 Proc Natl Acad Sci USA 87 2244 2248 Koepke JA and Landay AL 1989 Clin Immunol li 52 19 27 Kung P Goldstein G et al 1979 Science Laffon A et al 1991 J Clin Invest 88 19 Lanier LL Le AM et al 1983 J Immunol 20 Lum LG 1987 Blood 69 369 380 21 Maissen B Rebai N et al 22 Nadler LM Anderson KC et a 1987 Trans Proc 19 10 16 17 18 244 250 23 Polk B Fox R et al 1987 New LMed 316 61 66 24 Rebai N Maissen B 1982 ER Immunol 13 106 111 25 Reinherz EL and S an SF 1980 Cell 19 821 827 26 Reynolds CW 987 Immunology Today 8 172 174 27 Rouas Frel 97 Proc Natl Acad Sci USA 94 28 29 Eur J Immunol 15 88 91 1985 J Immunol 135 973 979 r GC Aoyama H et al 1984 Cell Diff 14 223 230 hoven A et al 1991 Eur J Immunol 1981 11 18 7elde et al 1991 Nature 351 662 665 SM et al 1991 Immunology 72 150 157 34 nke RA et al 1983 New Engl J Med 309 1275 1281 ola H 1987 Immunology Today 8 308 315 4
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