HPLC 用充填カラム Develosil ® XG シリーズ 取扱説明書
Contents
1. 6 pH pH pH1 5 8 pH8 7 0 45um 8 20MPa Ek 502. 9 0 10 Develosil HPLC 2 489 0004 15 Tel 0561 48 1853 Fax 0561 48 1434 Mail info develosil net Web http www develosil net Develosil XG series Packed Column
3. 4 0 125mm i d 0 15mm i d 0 20mm i d 0 25mm i d 0 8mm i d 1 0mm i d 5 0 45um
4. HPLC Develosil XG 1 Develosil HPLC Develosil HPLC 2 OFF 3
5. Instruction manual Introduction Thank you for purchasing our Develosil Packed Column We recommend you to read this manual carefully to ensure maximal efficiency and long life of the column Confirmation of columns Verify that there are no irregularities in the external appearance and packaging of the column Inspect the column to assure you received the correct column size and packing Handling of columns Do not drop or hit the columns because severe shocks can cause cracks or channels in the packing which can result in decreased column efficiency Installation of columns Before installing the column replace to the mobile phase which necessarily used the pump and the flow line Connect the column according to the flow direction indicated on the label To reduce dead volume be sure that the injector and detector connectors have a small diameter and are as short as possible Standard of Connector Diameter System type Connector Diameter Semi micro 0 125mm i d 0 15mm i d Conventional 0 20mm i d 0 25mm i d Semi Prep 0 8mm i d 1 0mm i d XThe above numerical value is standard In consideration of the rise of pressure be used of a proper connector Mobile phase Impurities in mobile phase cause ghost peaks and lead to noise as a result of loss of light intensity when using UV detectors If possible always use high purity solvents Check that the salt doesn t precipitate when mixed with org
6. anic solution before use Mobile phase containing insolubles should be filtered prior to use Degas all solvents prior to use to prevent malfunction of the pumps and to avoid detector noise caused by air bubbles Ifthe column operated at temperature near the boiling point of the mobile phase detector noise may be generated from the mobile phase bubbles pH Develosil XG series column are designed for use in the pH range of pH 1 5 8 Use under the alkali conditions exceeding pH 8 it is greatly undergone by the influence of the types the mobile phase composition and the temperature etc Sample Whenever possible samples should be dissolved in a solvent that has the same composition as the mobile phase Samples containing insolubles should be filtered prior to injection Analysis Keep pressure under 20MPa When use the preparative columns I D 20mm please confirm column pressure limit at test report Frequent changing of the mobile phase composition will accelerate deterioration of the packing It cannot be used of a methylene chloride by analysis and washing Storage of columns When dismounting the column be sure that the manometer reading is 0 If mobile phase containing buffer have been used the column should be first rinsed thoroughly with organic solvent water and then purged with acetonitrile water or methanol water before storage When storing columns first seal them with the plugs p
7. rovided and then place them in a stable temperature location Develosil Columns are manufactured inspected packaged and shipped under strict standards of quality control Should you find any defect in performance please contact us However that we can t guarantee the lifetime of columns nor can we accept any claim when their performance has deteriorated due to noncompliance with the above operating instructions NOMURA CHEMICAL CO LTD 15 Hinode cho Seto Aichi 489 0004 Japan TEL 81 561 48 1853 FAX 81 561 48 1434 E mail info develosil net
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