Cycleave®PCR Campylobacter ( jejuni / coli )
Contents
1. http www takara bio co jp 18 CY225 Epoch Biosciences Inc2. FAM ROX Primary Curve OUT PCR ROX FAM OUT gt Probe Mix BS i E E FAM ROX Primary Curve ND NN PCR
3. C jejuni C coli C fetus 11 1 ATccsi209 1 TERROR 4 Aggregatibacter pa actinomycetemcomitans 1 t a a a EE 1 37 EE ac Vibro parahaemolticus zemra on Koeee OD ooegeOAG eo geoseorege sgg 1 Csom aman O samona eo app o eomonmas pp eo Staphylococcus aureus 1 81 o 0 http www takara bio co jp 17 CY225 VIN 1 Worada Samosornsuk et al 2007 Microbiol mmunol 51 9 909 917 2 Asakura M et al 2007 Microb Pathog 42 174 183 IX Cycleave PCR O 157 VT gene Screening Kit CY213 Cycleave PCR O 157 VT gene Screening Kit Ver 2 0 CY217A B Cycleave PCR O 157 VT1 VT2 Typing Kit CY222 Cycleave PCR Salmonella Detection Kit Ver 2 0 CY205 Cycleave PCR Vibrio tdh gene Detection Kit
4. FAM ROX DNA ae no DNA Z Posi 10 CY225 Applied Biosystems 7500 Fast Real Time PCR System Life Technologies 1 2 3 4 5 6 7 8 9 Hold Cycle 1 95 C 10 3 step PCR Cycle 45 95 C 5 55 C 10 Advanced Setup New Experiment Experiment Properties Quantification Standard Curve TaqMan Re agents Other Other Include Melt Curve DY Plate Setup Define Target Target Name Jejuni Reporter FAM Quencher none Plate Setup Define Target Target Name Coli Reporter FAM Quencher none
5. 2 PCR 3 PCR 4 V 1 2 gt 1 E Lik 2 37 24 48 5 O2 10 602 85 N2 3 pola a CCDA RIRE al RE EIS 30 WML cued fale
6. DNA C jejuni cdtC gene DNA FAM C jejuni cdtC gene ROX DNA C coli Primer Probe Mix C coli cdtC gene DNA C coli cg7C gene DNA FAM C co7 catC gene ROX DNA lt C jejuni C coli cdtC gene DZ acd DNA DNA
7. 42 C 48 5 02 10 CO gt 85 N2 4 TE 10 mM Tris HCl 0 1 mM EDTA pH8 0 789 0 Aeoo nm 0 1 5 95 10 6 15 000 rpm 10 1 7 1 200 rom 59 Lox 93 15 000 rpm 10 2 TE buffer 95 10 AETA 3 15 000 rpm 10 NucleoSpin Tissue DNA PCR TE buffer
8. 1 C jejuni C jejuni cdtC gene FAM DNA ROX C coli C coli cdtC gene FAM DNA ROX FAM ROX 1 C jejuni cdtC gene C coli 010 gene DNA C jejuni C coli catC gene 1 C jejuni C coli PCR C
9. PCR PCR Co http www takara bio co jp 5 CY225 V 2 C jejuni cdtC gene C coli cdtC gene 1 C jejuni C coli PCR C jejuni 1 C jejuni cdtCgene DNA C jejuni C coli 1 C coli cdtC gene JV DNA C coli 1
10. ver 1 0 cE Thermal Cycler Dice Real Time System Software Ver 4 0 lt PM M Plus Minus Assay gt Thermal Cycler Dice Real Time System 2 FAM ROX PCR 3 Sten PCR 2 Hold Cycle 1 95 C 1039 3 step PCR Cycle 45 95 C 35 55 C 1039 72 C 20744 ltl 1 E CC 95 0 00 10 3 Start Run http www takara bio co jp 7 CY225 4 6
11. PCR Thermal Cycler Dice Real Time System TP900 TP960 Thermal Cycler Dice Real Time System Lite TP700 TP760 Ver 1 00 Thermal Cycler Dice Real Time System Software Ver 4 0 Applied Biosystems 7500 Fast Real Time PCR System Life Technologies 95 1 000 ul 200 ul 20 ul 10 ul BRKT 4 4 IV 1 PCR 2 3 4
12. 1 o 20 ul 1 5 ul 2 1 2 x Cycleave Reaction Mixture 1X C jejuni C coli Primer Probe Mix 5 X conc or C jejuni C coli Positive Control Or dH2O 2 5 Ul total 25 Ul k 5 ul 3 2 3 C jejuni C coli Positive Control 5 ul
13. 3 0 2 ml PCR 1 http www takara bio co jp 6 CY225 V 3 PCR 3 PCR fd Thermal Cycler Dice Real Time System Applied Biosystems 7500 Fast Real Time PCR System Life Technologies Thermal Cycler Dice Real Time System 1 Ver 1 0 Experiment Type lt CycleavePCR P M eee BITSA of CycleavePCR Kh Hees lt gt g wot x CycleavePCR Kit
14. warns an en 5 6 ThA OK OK Posi Nega Nega Nega Nega Posi Nega OK xrlo a m olol ol gt Nega Posi Posi Nega Nega OK http www takara bio co jp Negative Control lt N Positive Control lt P K OK OUT Unknown K U gt Posi Nega ND PCR Error i la FAM Primary Curve OUT
15. Plate Setup Define Target Target Name IC Reporter ROX Quencher none Define Samples NC PC 4 5 6 7 Plate Layout Passive Reference none Instrument Reaction Volume Per Well 25 Expert Mode Holding Stage Cycling Stage Number of Cycles 15 J Enable AutoDelta Starting Cycle F a 72 C 25 e Steh 1 Step 1 Step 2 Step 3 10 Start 11 Analysis HWA Amplification Plot Target Threshold Baseline Manual http www takara bio co jp 11 CY225 Amplification Plot 1 250 000 1 000 000 7 750 000 E 500 000 250 O00 3 4 6 amp 10 12 id i8 3 MO OO Cycle Lagend MA Ms Mic Mvp Mic
16. 1 gt CARO Wx oe DDO DODD ROX Omit C jejuni C coli C je juni C coli C jejuni Target Mark A C coli Target Mark B N IC NKN UNKN 104 NKN 14 B N KN KN IC U IC C jejuni C coli 1 2 5 1 http www takara bio coJp 8 CY225 FAM ROX 013 Auto C jejuni
17. PCR 4 KSI 6 PCR http www takara bio co jp 14 CY225 Vil VII 1 cfu colony forming unit C jejuni 81 176 C coli Col 243 C jejuni C coli PCR 1 cfu 1X 10 1 x 104cfu tube N 3 C jejuni xk 1 c
18. Tel 077 543 6116 Fax 077 543 1977 http www takara bio co jp DTI 42012090 9
19. DNA PCR DNA PCR DNA DNA dH20 WAK C jejuni Positive Control C jejuni catC gene C coli Positive Control C coli cdtC gene http www takara bio co jp 3 CY225 ll 20 C 1 4 5 6 20 Mh
20. CY220 Cycleave PCR Bacillus cereus CRS gene Detection Kit CY221 Cycleave PCR Listeria monocytogenes inlA gene Detection Kit CY223 Thermal Cycler Dice Real Time System TP900 TP960 Thermal Cycler Dice Real Time System Lite TP700 TP760 0 2 ml 8 strip tube individual Flat Caps NJ600 96 well Hi Plate for Real Time NJ400 Sealing Film for Real Time NJ500 48 well snap plate NJ700 Flat cap for snap plate NJ720 Plate Sealing Pads 9090 NucleoSpin Tissue 740952 10 50 250 X
21. Cjejuni C coli 2 PCR Hot Start PCR sia Ex Taq HS RNA DNA RNase H RNase H RNA
22. 2 C jejuni C coli Positive Control FP ROX DNA FAM C jejuni C coli 4 6 3 MARAKE AML ROX DNA Go FAM C jejuni C coli 1 DNA cdtC gene 2 DOC 3
23. Epoch Biosciences Inc US520020155484 WO0142505 WO02099141 US10 113 445 09 876 830 Epoch Biosciences Inc Epoch Biosciences Inc GE A B C _ MM NANOGEN Tel 077 543 6116 Fax 077 543 1977 http ww
24. 3 1 2 3 5 PCR PCR Auto PCR Manual http www takara bio co jp 4 CY225 V 1
25. C col C jejuni C coli 2 NC Positive Control NC FAM filter Manual ROX NC FAM NC ROX FET Ea RO PC FAM 7 4 JV 2 CRRA ROX 74 IV A lcSBUY BHA COSC ERTERT 4 PC FAM PC ROX 60 nevo 70 60 3 gt FAM http www takara bio co jp 9 CY225 6
26. Mone 35 9768 10 BE Sample 1 Jejuni UNKNOWN FAM None 28 308 11 By sample 1 Coll UNE NOW FAM None Undetermi 12 BY Sample 1 I UNKNOWN RO None 37 356 El Sample 2 13 CE Sample 2 LINE NOW ROx None 35 931 14 CE sample 2 Jeajuni UNE NMOVWE FAM None Undetermi 15 GT sample 2 Coli UNKNOWN FAM None Undetermi 16 Cr Sample 2 Ic UNKNOWN ROxX None 36 110 El Sample 3 17 DE Sample 3 Ic UNKNOWN ROx None 35 677 18 sample 3 Jejuni UNKNOWN FAM None Undetermi 19 D7 Sample 3 Coli UINKNOWN FAM None 30 258 20 Sample 3 I UNKNOWN ROx None 34 911 StepOnePlus Real Time PCR System Life Technologies ROX target ROX IC FAM ROX target http www takara bio co jp 13 CY225 Vi 1 ROX DNA aW BME J catC gene cdtC gene C jejuni C coli ase
27. jejuni C coli Primer Probe Mix E Ome Qh EES SSD ate einer http www takara bio co jp 2 CY225 L 25 ul C jejuni C coli 25 1 2 x 069 6 Reaction Mixture 2 X conc 625 ul 2 C jejuni Primer Probe Mix FAM ROX 1 5 Xx conc 125 ul 03 C coli Primer Probe Mix FAM ROX 1 5 X conc 125 ul O 4 dH20 1 ml 5 C jejuni Positive Control 2 50 ul 10 6 C coli Positive Control 2 50 ul 10 1 ROR WRAL TEEN 2 5 6 20 2 X Cycleave Reaction Mixture dNTP mixture PCR C jejuni Primer Probe Mix C jejuni cg7C gene
28. ding patents issued in other countries There is no implied license for commercial use with respect to this product A license must be obtained directly from Epoch Biosciences Inc with respect to any proposed commercial use of this product and commercial use includes but is not limited to A the sale lease license or other transfer of this product or any material derived or produced from it B the sale lease license or other grant of rights to use this product or any material derived or produced from it and C the sale lease license or other transfer of kits which include this product MK NANOGEN L15 Hot Start PCR Licensed under U S Patent No 5 338 671 and 5 587 287 and corresponding patents in other countries L42 Campylobacter PCR Detection This product is sold by Takara Bio Inc under license from Fuso Pharmaceutical Industries Ltd and Osaka Prefecture University This product is covered by the claims of U S Patent No 7 563 594 and its foreign counterparts M40 Thermostable RNase H This product is covered by the claims of U S Patent No 7 422 888 and its foreign counterpart patent claims M46 PCR Cycleave This product is covered by the claims of Japanese Patent No 4022522 M57 LA Technology This product is covered by the claims 6 16 of U S Patent No 5 436 149 and its foreign counterpart patent claims Takaka
29. e MF Mic WH Options Target Jejuni Threshold Auto 55454 224775 M Auto Show Threshold Raseline Start Well ll Tarnet Raseline gm 3 Jejuni Target FAM Amplification Flot 1 250 000 1 000 000 750 000 ARN 500 000 250 000 2 8 8 10 12 14 186 18 2310 22 24 38 28 34 8 38 40 44 Cycle Lagad MA Misa Mic E WF Mic EH Target lic Threshold Auto 131430 329036 M Auto Show Threshold Aaseline Start Aaseline JejuniIC ROX http www takara bio co jp 12 CY225 12 View Well Table gt View Plate Layout View Well Table select Wells With select Item showin Table Group By wen Sampien TargetNa Task Dyes er NC 1 AG NC I NTC ROx Mone 37 602 Standard Curve 2 AG NC Jejuni NTC FAM None Undetermi 3 Af Nc Call NTC FAN NMone WUndetermi m 4 Ar NC I NTC ROx Mone 37 889 ea Mutticomponent Plot PC 5 He fee IC LINE NOW ROx NMone 38 126 He HE Jejuni FAM None 26 620 H7 PG Coli UNKNOWN FAM None 25 218 H7 PC Ic UNKNOWN ROx NMone 38 889 Sample 1 g BE Sample 1 Ic UNENOWN ROx
30. gaa Y 5 Cycleave PCR Campylobacter jejuni coli Typing Kit v201209Da 3 10 90 Campylobacter jejuni Campylobacter coli 1 lt 2 1 PCR Cytolethal distending toxin cdat genes C cdtC gene
31. lu http www takara bio coJp 15 CY225 VII 2 C jejuni C coli C jejuni 186 C coli 65 C jejuni 186 185 99 5 C coli 65 65 100 C jejuni 1 cdt 5 1 NN esseo Oi tT Access 1 81176 1 2 172 cdt 5 4 6 6 186 185 0 ATCC33559 1 1 esao ERRORE OD 27 1 23 12 12 65 0 65 http www takara bio co jp 16 CY225 Il 3 C jejuni C coli C jejuni C coli 81
32. wr takara bio coJp http www takara bio co jp 19 CY225 NOTICE TO PURCHASER LIMITED LICENSE L4 Quencher This product is for measurement of amplification detection for research use only in life sciences research industrial and environmental testing including food industry but excluding bio terrorism and bio warfare non human ani mal diagnostic testing forensic testing and providing services to third parties who do not use the services for the purpose of a providing patient management or care and in which the results of such services are not included in patient records and b providing bio terrorism or bio warfare testing and specifically excluding without limita tion I any human clinical therapeutic or diagnostic uses and animal clinical and therapeutic uses including any use of the results of any testing performed with any product for patient management or care or the use of the results of the services for patient management or care and Il any research or services where the results of any test or assay are used for patient management care or otherwise in making therapeutic or treatment decisions for a patient A portion of this product is subject to proprietary rights of Epoch Biosciences Inc and are made and sold under license from Epoch Biosciences Inc under the patents and patent applications US20020155484 WO0142505 WO02099141 US10 113 445 US09 876 830 and correspon
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