JP 2008-520193 A 2008.6.19 10 (57)【要約】 全
Contents
1. 10 20 30 40 50 166 2008 520193 A 2008 6 19 RAB M GPAT2. 10 20 30 40 50 59 2008 520193 2008 6 19
3. 10 20 30 40 50 2008 520193 A 2008 6 19 125 1 1 10000 1 1 10 20 000
4. BOSE B E ER ET LI HHHHHH 10 1 1111 11 E B dt dt Uu UH U dt A U dt IHHHHHHH 20 22 pKUNT2 116 116 RE m Ascl BsiWI poor Ura3 AJ306421 3225 3015 784bp 5 Sphl Pacl Ura3
5. N N N N N r1 r1 r1 L 10 20 30 40 50 185 2008 520193 A 2008 6 19
6. C CO U 000000 000000 00 000 00000060 U LL U U U HHnnnnn LL 18 pZKUT16 159
7. 1 1 D 1 D 10 20 30 40 50 2008 520193 A 2008 6 19 64 10 1 20 30 40 50
8. 10 20 30 245 159 LPAAT2 Y2067U JP 2008 520193 A 2008 6 19
9. A A D 0 A Ove RE 8 10 20 30 40 50 2008 520193 2008 6 19 43 10 20 30 40
10. 10 20 30 40 50 62 2008 520193 2008 6 19 00000000000000000000000000000000000 HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH IHHHHHHHHHHHHH HHHHHHHHHHHHHH 0000 IHHHHHHHHHHHHHH HHHHHHHH HHH
11. A U A A A A 1 00000000000 11 11 A
12. 10 20 30 40 50 2008 520193 A 2008 6 19 8 10 20 30 40 50
13. 0 0 2008 520193 A 2008 6 19
14. 40 3 O MAI a ES de 7 17 EE IE ES y ESTE pre O E3 AT MEME O o _ U o O o O o O ie 2008 520193 2
15. cm n ipe DUM C N mI N N N C H H 10 20 30 40 50 96 2008 520193 2008 6 19
16. 10 20 30 40 50 191 2008 520193 A 2008 6 19 20 2008 520193 A 2008 6 19 192 lt 8 lt a e lt cxre LAA zn 8 lt YAEAM AA tyag szzol acres yy vaa szc
17. 2008 520193 2008 6 19 69 10 20 30 40 50 1 EE ol
18. 10 20 30 40 50 2008 520193 A 2008 6 19 88 10 1 1 1 20 30 40 50
19. lt gt e s 10 20 30 40 50 155 2008 520193 2008 6 19 C n n n x C X Lm m SEG x C nm
20. 10 20 30 40 50 55 2008 520193 2008 6 19
21. 10 20 30 40 13 2008 520193 A 2008 6 19 IHHH pY89 5 ATCC PTA 6048 2004 6 4 Y2047 ATCC PTA 2096 ATCC Y2201 ATCC PTA _ Y3000 ATCC PTA 10 UU 5 U
22. 30 40 50 87 2008 520193 2008 6 19 C
23. pa B oogagu 00000 0 s u i Annnnn E n A 10 20 30 40 50 2008 520193 2008 6 19 34 10 n 1 20 1 30 40 50
24. UU HHHHHHHHHH HHHHHHHHHHHHHHHHHHHHHHHHHH ood 00000 HHHHHH 0000000 HO O C IHHHHHHHHHHHH LL U Od U 1 0000000000000 U 10 20 30 40 50 154 2008 520193 2008 6 19 gt
25. HHHHHH HHHHHHHHHHHHHHHHHHH 1 1 1 HHHHHHHHHHHHHHHHHH 1 HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH etas ET S A nnnnnnnAnnnn A A INN GTC es ENS n HHHHHHHHHHHHHHH um 20000000 10 20 30 40 50 2008 520193 A 2008 6 19 163 10 20 30
26. 2008 520193 A 2008 6 19 65 10 20 30 40 50 1
27. 2008 520193 A 2008 6 19 35 10 20 30 40 50 D D L3 oo um C C D C D D D O 1 C D SS D O
28. 2008 520193 2008 6 19 82 10 20 30 40 50 83 2008 520193 A 2008 6 19 1 E m
29. 000000000 81 883809 T D 00000000 54 pZUF6FYE1 203 203 RE EcoRI Clal 18 ARS18 7047 8445 M91600 Sall Pacl Ura3 AJ306421 1493 1 EcoRI BsiWI pZUF6 22 1534 4251 FBAIN A6S Pex20 Clal Pacl FBAIN YE1 Aco 8443 1 e FBAIN FBAIN 214 e YE1 YE1 97 CAG83378 Aco3 Aco3 AJ001301 HHHHHHHH HHHHHHHHHHHHHH 00000000 0
30. A A 10 20 30 40 50 46 2008 520193 A 2008 6 19 D 00000 x E 00
31. 10 20 30 40 53 2008 520193 A 2008 6 19 IHHHH 6 A12 A15 His Box 1 45 107 118 Mad12 46 116 127 Skd12 41 153 lt 164 Nc d12 33 149 gt 160 Fm d12 8251385 3900160 171 Mg d12 35 143 154 An d12 43 130 141 Fg d12 48 106 117 Kld12p 49 135 146 Cad12p 50 120 131 Dhd12p
32. 10 20 30 40 50 2008 520193 2008 6 19 73 1 0 10 20 30 40 50 n 1 0 ol
33. 30 40 50 2008 520193 2008 6 19 178 10 20 C 30 C C 40 50 C C C C C
34. 2008 520193 A 2008 6 19
35. 1 1 1 1 40 1 1 95 2008 520193 A 2008 6 19 12 TEF FBAIN YAT1 GPAT FBAIN 48 SD 43 333 24 HGM 30 694 120 HGM 17 200
36. 134 2008 520193 A 2008 6 19 30 pY72 176 176 RE 6763 7643 Lip1 Z50020 881bp 5 9422 10184 Lip1 Z50020 763bp 3 Swal Sbfl FBAIN FmD15 Lip2 16 2522 FBAIN FBAIN 214 FmD15 A 15 51 e Lip2 Lip2 AJ012632 Lip2 2531 2564 LoxP 382 2566 4184 Ura3 AJ306421 4198 5861 EURESACHATEF HPT XPR e TEF TEF AF054508 e H
37. 129 2008 520193 A 2008 6 19 HHHHHH IHHHHHHHHHHHHHHHHHHHHHHHHH HHHHHHH HHHHHHHHHHHHHHHHHHHHHHHHHH IHHHHHHHHHHHHHHHHHHHHHHHHH HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH IHHHHHHHHHHHHHH A AHHHHHHHHHHHHAHHH 0000000000000 27 pDMW314 173 173 RE Ura3
38. 171 2008 520193 A 2008 6 19 550 ELO3 Y2031 Y Y2031 16 0 16 1 18 0 18 1 1 3 pZUF6S xt IR 9 4 23 1 39 3 pZUF6S E3WT 3 8 7 18 3 4 1 37 1 20 0 pZUF6S E3WT 5 8 9 17 2 4 0 37 6 21 0 pZUF6S E3WT 6 36 3 20 5 12 1 26 5 73 5 pZUF6S E3WT 7 12 0 25 6 74 4 C16 C18 ip ik uu
39. 25 2008 520193 A 2008 6 19 10 20 30 40 50 2008 520193 A 2008 6 19 26 1 1 1 10
40. 1 0 160 RE 109 19 pKO2UM25E 160 Hindlll Ascl A12 28 728bp 1 728 5 Sphl EcoRI A12 28 556bp 3436 3992 3 BsiWl Hindlll GPAT EL1S XPR 10437 1 GPAT GPAT 216 e EL1S 1 24 AX464731 e XPR Xpr M17741 100bp 3 Bglll BsiWl FBAIN M A12 Pex20
41. lo conu 00000 1 1 ol ol 2008 520193 2008 6 19
42. 10 20 30 40 50 2008 520193 A 2008 6 19 146 1 1 1 10 C 20 C 000 0 30 40 lt
43. 2008 520193 2008 6 19 117 10 20 30 40 50
44. H H uHH C m EE zu C C C C 10 20 30 40 50 97 2008 520193 A 2008 6 19
45. HHHHHHHHHHHHHHHHHHHHHHHHHHH HHHHHHH HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH IHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH HHHHHHHHHHHHHH HHHHHHHHHHHH 000000 HHHHHHHHHHHHHHHHHHHHHHHHHH IHHHHHHHHHHHHHHHHHHHHHHHHHH IHHHHHHHHHHHHHHH HHHHHH H HHHHHHHHHHHHHHHHHHHHHHHHHHHH oo00000000000000000000A0 HHHHHHHHHHHHHHHHHHHHHHHHHH HHHHHHHHH HHHHHHHHHHHHHHHHHHHHH 21 pZP2FOEN4 165 165 RE Aco2 AJ001300 6152 6962 810bp 5 Aco2 AJ001300 9670 10325 655bp 3 EcoRI Pacl YAT MS Pex16 10640 2648 e _YAT YAT1 217 A4S A4 106 AAN75707 e Pex16 Pex16
46. A 2 3 2 ooann Ann 10 20 30 40 50 58 2008 520193 A 2008 6 19 A Lt m E
47. 50 105 2008 520193 A 2008 6 19 HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH IHHHHHH HHHHHHHHHHHHHHHHHHHHHHHHHH HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH HHHHH HHHHHHHHHHHHHHHHHHHHHHHHH A E B BD 010111 a B E SET ETUR ELE 000000000000000000000000000000000 HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH 16 pDMW271 157 157 RE Ascl BsiWl Leu2 AF260230 5520 6315 788bp 5
48. 20 1 1 1 1 1 1 1 1 A 1 1 1 145 2008 520193 2008 6 19 39 pDMW232 182 182 RE Ascl BsiWI Leu2 AF260230 788bp 5550 4755 5 Sphl Pacl Leu2 AF
49. 2008 520193 A 2008 6 19 78 10 20 30 l 40 50 2008 520193 A 2008 6 1
50. 157 2008 520193 A 2008 6 19 ed u u C C C C C z ID ID ID Bn gt 0 00000 10 20 30 40 50 158 2008 520193 2008 6 19
51. 10 20 30 40 50 2008 520193 2008 6 19 93 10 20 30 40 lt lt C 50 supp C 2008 520193 2008 6 19 94 10 20 30
52. 40 161 2008 520193 A 2008 6 19 IHHHH 46 M DGAT1 Y2067U 18 0 18 1 18 2 GLA DGLA ETA Y2067U pZUF MOD 1 1 1 31 6 92 12 03 23 11 5 72 1 05 3 80 13 20 Y2067U pZUF MOD 1 2 1 39 6 83 12 15 21 99 5 63 1 07 3 82 13 47 14 09 15 07
53. C E 521 E E 10 20 30 40 50 2008 520193 A 2008 6 19 139 A 1 1 IUDA 10 20 30 1
54. 43 pZKUGPE1S 187 187 RE BsiWI Pacl Ura3 AJ306421 721bp 318 1038 5 Sall Clal Ura3 AJ306421 724bp 3882 4606 3 Clal BsiWI GPAT E1S Pex20 4606 318 e GPAT GPAT 216 e ELIS 1 24 AX464731 Pex20 Pex20 Pex20 AF054613 C
55. 50 0 0 12 2008 520193 A 2008 6 19 POPP gt HHHHHHHHHHHHIH
56. 10 2008 520193 A 2008 6 19 84 10 20 30 40 e voe zzz ss e 88 4 4 e 6 se L6 e Le rp e per rep Z e eme Ee en 651 8SL 01 6 kee iz ze dl Sl REZ ROL PJ 1 COCR HOMEY E Lya DATA d 9 f LOVI M 50 000 85 2008 520193 A 2008 6 19 A D 10 20 30 40 50 2008 520193 A 2008 6 19 86 10 20
57. 1 1 lt l 2008 520193 A 2008 6 19 116 10 20 30 40 50
58. 10 11 1 20 30 1 40 1 1 U n 50 2008 520193 A 2008 6 19 39 10
59. 259 DGAT2 EU ITFAI 1 1 16 0 16 1 18 0 18 1 18 2 DGLA ARA ETA EPA dcw EU 19 10 2 86 12 6 3 to 3mL EU D2 1721416104 6 19 3mL 37 6 11 3 31 5 1 simL pc 2 12 9 1 43 7 3 5 4 1 24 14 0 4 37 5 0 1 34 18 12 3 21 29 5 1 oe EU D2 5 5 20 51mL PC NEUE 22 PE 2 8 8 1 615 21 10 20 30 40 50 2008 520193 2008 6 19 187 10 20
60. 2008 520193 2008 6 19 37 1 1 1 1 10 n 20 30 40 50 2008 520193 2008 6 19 38
61. 111 l ID 30 st W AHAS O st 2 lu 812 S m E E lt 8 quss 1 HH H m 2 2 juju eo H m 9 E lt H m DNA 50 1 1 U 1 1 1 1 1 00000 2008 520193 A 2008 6 19 100 10 20
62. 10 20 30 40 50 51 2008 520193 2008 6 19 10 20 30 40 50 52 2008 520193 A 2008 6 19
63. 20 30 40 50 40 2008 520193 A 2008 6 19 A 10
64. LI CI CI LL DO 10 20 30 40 50 151 2008 520193 2008 6 19 0 A 000000 0 A A A A A A A A A A oe A U
65. CPT1 149 2133 bp CPT1 150 1185 bp 152 153 C ISC1 1434 bp 477 AA 3 210 m GPD 971 bp 10 30 40 18 1 1 U FBA 3 211 GPDIN 1174 bp 212 GPM 878 bp 2008 520193 A 2008 6 19 213 1001 bp
66. 0000000000000 A 00000000080 UE 0000 0000000000000 00000 HH 000000 0 0 HH 0 IHHHHHHHHHHHHH HH L L m Y tl HHHHHHHHH 0000000000000 1 1 U 100 000 000000 HHHHHHH d 10000 HHHHHHHHHH HH 000000100 UU U zm BE s D 1 10 20 30 40 50 23 2008 520193 A 2008 6 19
67. 50 2008 520193 A 2008 6 19 77 10 20 30 40 50
68. 10 20 30 40 50 C C C C C C 169 2008 520193 A 2008 6 19 1000000000000 10 170 2008 520193 A 2008 6 19 549
69. 179 2008 520193 A 2008 6 19 H 10 20 56 Y CPT1 Y2067U 0 18 1 18 2 GLA DGLA ARA ETA Y2067U pZUF MOD 1 1 13 6 9 120 23 1 8 7 11 3 8 Y2067U pZUF MOD 1 2 14 68 12 1 220 56 11 3 8 Y2067U pYCPT1 ZP2I7 1 0 6 8 0 82 274 7 1 16 41 30 Y2067U pYCPT1 ZP2I7 2 06 8 1 8 2 272 70 16 4 0 Y2067U pYCPT1 ZP2I7 3 9 80 247 61 16 32 Y2067U pYCPT1 ZP2I7 4 0 6 7 1 86 255 69
70. DGAT1 DGAT2 DGAT2 3 GPAT M GPAT cDNA M GPAT 1050bp 2886bp M GPAT 3 cDNA 129 458 AA 130 2119 bp 131 514 132 1380 bp 133 459 AA 134 1068 bp 135 355 AA 136 996 bp 137 331 AA 138 139 2151 bp 716 zo 1212 bp 141 3935 bp 142 965 bp M GPAT 5 M GPAT 143 1908 bp 144 966 bp M GPAT 1 M GPAT 2 145 275 bp M GPAT 3 M GPAT 4 146 255 bp 147 83 bp 148 99 bp
71. 1 1 0 30 40 50 2008 520193 A 2008 6 19
72. 2008 520193 2008 6 19 118 10 20 30 40 50 C 2008 520193 A 2008 6 19 119 10 C 1 1 20
73. D L3 L3 L3 Lp CET l 10 20 30 40 50 110 2008 520193 A 2008 6 19 0000 C 0 10 20 30 111 2008 520193 2008 6 19 20 pDMW303 161 161 RE BsiWI Ascl 1 Z50020 1 819 819bp 5
74. i 30 T x 40 56 J P 2008 520193 2008 6 19 7 DHA 10 M A6 152 1374bp 42 3 144 04 101753 AF465281 M 94 957bp Cszo 85 04 101753
75. Muller TEF I 1 218 EF1 a 6 265 185 AF054508 968bp 3bp 3 E 210 2005 003310 875bp 3bp 212 Ee TEET 2005 049805 P OP 804bp 169bp 102bp 2005 049805 dm 64 165 5 ZNBE SE SENE 804bp 169bp REZ 2005 049805 973bp 201bp t 465p 49 194 11 183664 d 3 IJ 77 8 11 225354 9488 216 778 1b 217 11 185301
76. 66 2008 520193 2008 6 19 W W H AA 1 10 20 30 40 50 2008 520193 A 2008 6 19 HHHHHHHHHHHHHHHHHHHH HHHHHHHHHHHHHHH HHHHHHH HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH 9
77. 147 2008 520193 A 2008 6 19 40 pKO2UF2PE 184 184 RE Ascl BsiWl 3382 2645 A12 28 730bp 5 Sphl EcoRI 6090 6646 Swal BsiWIl 17 2645 A12 28 556bp 3 FBAINm F A12DS Pex20 FBAINm FBAINm 215 e F A12 A12 32 Pex20 Pex20 Pex20 AF054613 Swal Pmel 178525 EcoRI Pacl 6646 8163 GPAT EL1S OCT e GPAT GPAT 216 e EL1S 1 24 AX464731 OCT OCT OCT
78. E nnnnmn E n m E EE E 10 20 30 40 50 2008 520193 A 2008 6 19 A 10 20 30 40 50 61 2008 520193 A 2008 6 19
79. 10 20 30 40 50 2008 520193 A 2008 6 19 5 2 8 a 5 a a lt 53 lt r 3 Ez lt Oo lt ol a cii LI a a lt lt lt a a a a lt a ol a ol E
80. 2008 520193 2008 6 19 70 10 20 30 40 50 2008 520193 A 2008 6 19 71 10 20 n
81. 1 1 30 lt 2008 520193 A 2008 6 19 188 10 20 30 40 9 0 0 d za NW 3 9 d NW 3 2 0 9 aay iz 3 r 8 z nal 3 r OL 9 zi 92 AWW 9 2 8 9 LS G 2 Wey a L OL l c OOL i IAL r 9 9L ZL 8 5 1 ze 29 WOH AWA 8 2 2 4 0 06 Al ee HEY HAL 8 el 001 9 1 L Z 9c dnN v 2 0 z IS 0 La nw 108 8 0 1 o 5 zs vor IET Za nW Y L6 4 z 01 L 00 VOZ 21 y a se
82. 183 2008 520193 A 2008 6 19 HHHHHHHHHHHHHHHHHHH 20 30 40 50 184 2008 520193 A 2008 6 19 n E SERE nm CM CE EC
83. 30 40 50 2008 520193 2008 6 19 101
84. 30 40 29 2008 520193 A 2008 6 19 33 zh 14 0 Palmitate 16 0 9 16 1 18 0 9 9 12 6 9 12 18 3o 6
85. AA HHHHHHHHHHHHHHHHHH 0 00000000000000000000000000000000 DO0 00000000000000000000000000000001010 3 13 GUS XPR 1 2 FBAIN GPM 1bp FBAIN 1bp 171bp DMW224 219 843bp 62bp 165bp P GPM GPDIN GPM 1bp GPDIN 1bp 198bp D225 220 843bp 49bp 194bp ETE EES 10 20 30 40 50 2008 520193 A 2008 6 19 98
86. U U 5 gu 0000 20 U U U U U 1 30 A6 1 1374 bp 2 457 AA 3 1374 bp 2 457 AA A6 A6 B 4 1521 bp 5 458 AA 14 2008 520193 A 2008 6 19 A5 6 1341 bp 7 446 AA A5 8 1329 bp 9 442 AA 10 1329 bp 9 442 AA A5
87. 27 819 bp 26 272 AA Crazo A12 28 1936 bp 29 419 AA A12 30 1203 bp 31 400 AA A12 32 1434 bp 33 477 AA A12 34 1416 bp 35 471 AA Aspergillus flavus A12 36 466 AA A12 37 424 A12 38 1656 bp 39 551 AA A12 40 1446 bp 41 481 AA A12 42 1371 bp 43 456 AA A12 44 1403 bp 45 400 AA A12 46 416 AA A12 47 1948 b
88. z 8 8 5 a 3 SEE E L3 F3 r3 3 oo 3 FAT E3 EIE EXCESS V ooog Soe oe pas DI I F3 r3 r3 Ol Ol E E E r3 9 E ooo oP oog DI I oa 9 9 9 Ol Ol O I 9 9 DI I lt lt p 9 2008 520193 2008 6 19 102 10 20 C 30 40 50
89. 10 20 30 40 50 7 2008 520193 A 2008 6 19 1111 111 1 11 1 1 1111 1111 1 1 l HHHHHHHHHHHH 1111 111 1 1 1 111 1 11 Uu 1 111 111 1 1 1 111 1111 111 1 11 1 iu 11111 1 1 1 1 1 1 11 1
90. 50 ol ol 4 44 2008 520193 A 2008 6 19 DHA co 6A6 A6 oo 3A6 A6 DHA A6D ELO ABD A17D 0202 ELO A4D A15D A6D ELO ASD 02022 ELO A4D PUFA o 3 o 6 PUFA oi 3 oo 6 GLA A6 A6 A15D A6D ELO A5D A17D ELO A4D
91. 10 20 30 40 50 2008 520193 2008 6 19 28 10 20
92. 2008 520193 2008 6 19 90 000 10 20 30 00 40 91 2008 520193 A 2008 6 19
93. 1 20 30 1 00 40 1 000 50 2008 520193 A 2008 6 19 27
94. l 30 1 171 RE 126 JP 2008 520193 A 2008 6 19 25 pDMW297 171 EcoRI Clal 9053 10448 Clal Pac 14 2590 ARS18 A17608 FBAIN D8SF Pex16 e FBAIN FBAIN 214 e D8SF A08 81 AF139720 e Pex16 Pex16 Pex16 U75433 Pacl Sall 2590 4082 Ura3 AJ306421 Sall BsiWl 4082 6257 FBAIN lgD9e Pex20 e FBAIN FBAIN
95. RE EcoRI Clal 18 ARS18 3114 4510 M91600 Sall Pacl Ura3 AJ306421 6022 4530 EcoRI BsiWI FBAIN A6S Pex20 6063 318 FBAIN FBAIN 214 65 A6 3 AF465281 20 Pex20 Pex20 AF054613 UU 52 pZUF6TYE2 201 201 RE EcoRI Clal 18 ARS18 7461 8857 M91600 Sall Pacl Ura3 AJ306421 1907 415 EcoRI BsiWI pZUF6 FBAIN 1948 4665 65 20 Clal Pacl TEF
96. 20 30 40 50 2008 520193 A 2008 6 19 11 nnnnm 10 20 30 UUU U U 40
97. 85 804 bp 84 267 C1 erg C1ersg ELO3 86 828 bp 87 275 AA 20 30 16 2008 520193 A 2008 6 19 ELO3 ELO3 cDNA 88 607 bp 89 1042 bp 05 3482 90 2223 bp ELO3 5 ELO3 cDNA 91 3557 bp ELO3 92 542 bp ELO3 93 4099 bp Ciengs 94 oi 5 bp 95 304 AA 96 353 AA EAL04510 C441fs 97 978 bp 98 325 AA FEN1 99 337
98. 10 20 30 40 50 2008 520193 A 2008 6 19 152 1 10 20 1 00000 153 2008 520193 A 2008 6 19 188 RE Hindlll Ascl 17728 A12 28 728bp 5 Sphl EcoRI 3436 3992 A12 28 556bp 3 BsiWl Hindlll
99. C C 121 2008 520193 A 2008 6 19 H 0 H u C C C C C C H i e 5 ae Ug dp B Ug
100. 51 e Xpr M17741 100bp 3 Fsel Sbfl PY72 FBAIN FmD15 Lip2 2385 4891 4900 4933 LoxP 382 4935 6533 Ura3 AJ306421 6567 8230 pY72 TEF HPT XPR 8231 8264 LoxP 382 8271 9079 Lip1 Z50020 809bp 5 11791 12553 Lip1 Z50020 763bp 3 HHHHHHHHHHHHHHHHHHHHHHH 1 U 1 U 2532 pY79 178 178 RE 4329 7315 W497L AHAS 7362 1 243 TEF Cre XPR e TEF TEF
101. HHHH 128 2008 520193 A 2008 6 19 R26 pZP2C16M899 172 172 RE BsiWl Ascl Aco2 AJ001300 810bp 6152 6962 5 Sphl EcoRI Aco2 AJ001300 655bp 9670 10325 3 EcoRV GPM FBA rELO2S Oct BsiWI Pmel GPM FBAIN GPM FBAIN 219 929 3195 ELO2S AB071986 IELO2 85 e OCT OCT OCT
102. 0000 1000006060 0000 111 1 1 DU DUHH U 11 11 1 11 U 1 0000000000000
103. 1 1 000 2008 520193 A 2008 6 19 76 1 1 10 20 30 40
104. 10 20 30 40 50 n 2008 520193 A 2008 6 19 99 1 10 20
105. 10 20 30 40 C 50 C 2008 520193 A 2008 6 19 31 10
106. 2008 520193 A 2008 6 19 21 N Big ES E mool 30 1 Il 40 1 1 50
107. ol 74 2008 520193 A 2008 6 19 10 20 30 40 50 2008 520193 2008 6 19 75 10 20 30 40 50 1 1
108. 115 116 LPAAT2 672 bp 223 AA 117 118 PDAT 2326 bp 648 AA CoA 119 120 ARE2 1632 bp 543 AA 10 20 30 40 17 CoA 1 LPCAT 2008 520193 A 2008 6 19 DGAT1 DGAT1 DGAT1 122 1578 bp 124 125 1578 bp 525 AA PH 1 DGAT1 DGAT1
109. 58 PDAT DGAT2 DGAT1 ATCC 90812 1 1 3 TAG dew FAs FA dc FA dcw FAs FA IFA dcw 100 723 136 se 617 116 83 SP pp 288 318 60 38 212 40 29 50 02 p 66 29 41 26 t3 21 15 IHHHHHHHHHHHHHHHHHHHH HH HHHHHHHHHHHHHHHHHHHHHHH IHHHHHHHHHHHHHHHHHHHH IHHHHHHHHHHHHHHHHHHHH HHH HHHH HHHHHHHHH HHHHHHHHHH HHHHHHHH HH HHHHHH HHHHH IHHHH HH 0000 HHHHHHHHHHHH HHHH HHHHHHHHHHHH I00000000000 IHHHHHHHHHHH HHHHHH HHHHHH HHHHHHHHH HHHHHHHHHHHH 10 20 30 40 50 186 2008 520193 A 2008 6 19
110. X69988 C 10 20 30 40 50 2008 520193 2008 6 19 148 000 10 1 000 20 l 30
111. 2008 520193 A 2008 6 19 9 10 20 30 40 50 1 1 0 1 2008 520193 A 2008 6 19 10 10
112. D L3 L3 L3 0O D L3 L3 D 1 LI 20 30 40 50 162 2008 520193 2008 6 19 RAT DGAT2 Y2067U 18 0 18 1 16 2 GLA IDGLA ETA EPA 2067U pZUF MOD 1 1 1 31 6 92 12 03 23 11 5 72 1 05 3 80 2067U pZUF MOD 1 2 1 39 6 83 12 15 21 99 5 83 1 07 3 82 2067U pMDGAT2 17 1 0 00 7 47 10 77 25 30 5 70 1 43 3 45 2067U pMDGAT2 17 42 1 45 7 79 9 96 25 16 6 06 1 25 3 99
113. M34929 0687bpO 10 13804 13117 3 BsiWISwal NT 1 058 7150 9573 e NT YAT1 217 e 55 A5 10 2002 081668 Lipi Lip1 Lip1 Z50020 Sall BsiWl GPAT MA A 5 Pex20 4537 7150 e GPAT GPAT 216 MAAS A 5 20 6 AF067654 e Pex20 Pex20 Pex20 AF054613 Swal Pmel FBAINm IgD9e OCT 2381 5348 e FBAINm FBAINm 215 lgD9e galbana A 9 71 e OCT OCT OCT X69988 Clal Pacl GPD D8SF Pex16 29 1713804 e GPD GPD
114. 40 1000000000 1 1 149 2008 520193 2008 6 19 187 RE BsiWI Pacl Ura3 AJ306421 721bp 318 1038 Sall Clal Ura3 AJ306421 724bp 3 3882 4606 Clal BsiWl GPAT 1 A5S Pex20 4606 318 e GPAT GPAT 216 e 1A5S A5 10 2002 081668
115. 10 20 30 40 50 2008 520193 A 2008 6 19 182 10 1 1 1 1 20 30 40 50 lt
116. 214 e IqgD9e A9 71 390174 e Pex20 Pex20 Pex20 AF054613 LIL D L3 L3 L3 gt gt gt 10 20 30 40 50 2008 520193 A 2008 6 19 127 1 1 1 1 1 10 20
117. CAD70918 C2ozs 102 803 x dos C2o gt A4 104 1548 105 515 106 1545 bp A4 108 945 bp 109 LPAAT1 314 AA 110 927 bp LPAAT2 112 LPAAT1 1549 bp 114 LPAAT2 1495 bp
118. 1 1 156 RE 104 2008 520193 A 2008 6 19 15 pKUNF12T6E 156 Ascl BsiWl 9420 8629 Ura3 AJ306421 784bp 5 Sphl Pacl 12128 1 Ura3 AJ306421 10 516bp 3 Swal BsiWIl 6380 8629 Bglll Swal 4221 6380 FBAIN EL1S Pex20 FBAIN FBAIN 214 EL1S 1 24 AX464731 Pex20 Pex20 Pex20
119. 10 20 30 40 50 4 2008 520193 A 2008 6 19 Uu A Uu IHHHHHHHH E 1000000000 UU P HHnnnnn HHnnnnn A HHnnnnn HHHHHH sri as
120. I0000000000000 I0000000000000 H IlHHHHHHHHHHHHH 10 20 30 40 50 24 2008 520193 A 2008 6 19 10 20 30 IHHHHHHHHHHHH 40
121. 20 30 40 50 A HH U 2008 520193 2008 6 19 32 10 20 1 30 40 50 HHHH 0
122. 2008 520193 A 2008 6 19 112 10 20 30 40 C 1 50 C C C 113 2008 520193 A 2008 6 19
123. 28005 AAO5 BAO1 BAO5 DOL DAO5 GO1 GO2 GO6 MX2 28150 A02 AAO5 AMG AAO7 4 0337 2 TP6 48016 1806 45024 AAO1 DAI2 4B026 0005 45064 ADI2 EM5 LB10 AALO FA02 CAL9 07 All 24 206 MBl MO2 BASO CAOL HA08 DO1 310 45065 AAO1X AA57X AA72X AA72Y AAB7X ABOL CA43 CAM 40087 01 AN BI1 M52 M4 ZO 2 2 4006 01 AM DOS 9 MO ZA59 221 2 39 45559 BBO7 BUS ZA12 4 M13 5 ZA05 2008 520193 A 2008 6 19 DAO5 2459 421 ZAD 2 5
124. 18 0 18 1 18 2 GLA DGLA ARA ETA Y2067U pZUF MOD 1 1 1 1 4 7 109 194 63 09 3 9 13 8 Y2067U pZUF MOD 1 2 4 0 14 1 Y2067U pMLPAT 17 1 3 4 15 5 20670 17 2 2 9 16 0 Y2067U pMLPAT Int 1 19 49 139 211 48 1 1 2 7 16 6 Y2067U pMLPAT Int 2 1 7 4 2 12 1 21 3 5 2 12 2 9 173 poda z gt B H pakaq ys x C C C C C 10 20 30 40 50 160 2008 520193 2008 6 19 10000 H 20
125. AX464731 20 T 114 817bp aureum Cig LAY 108 ELO2 S A17 127 1077bp 117 107840478 2003 0196217 A1 126 789bp A9 123 30 A8 207of1263bp 192 11 166993 203 1323bp A5 193 A5 227of1335bp 207 NP_037534 170 1545bp A4 166 40 160 903bp 472 Cao 147 C ss 127 792bp 125 AB071986 50 57 2008 520193 A 2008 6 19
126. Pex20 BsiWI 318 Ura3 3 1038 Sall 5705 pZUF MOD 1 7323 bp 201 JP 2008 520193 A 2008 6 19 rELO2S Pex20 8sil 1 EcoRI 113328 ipi 5 56081 13306 Lipi 3 4 4 Sall 12679 D65 Sphl 3525 FBAIN pDMW302T16 BglII 10599 14864 bp Pmel 10555 Lipt 3N ef 4294 16 3 Sail 9437 lt EL1S 225 3 F D12 Sall 8881 SS ter Lip2 3 16450 Clo 8078 uray 6450 Sall 8069 FIG 17C XPR2 Nott 13151 1 126681 YD12N Ncol 112171 2 126 5891 11133 BsiW 11095 Amp Pex20 3 10778 MA D12 pKOZINESE Sphl 3436 N 512 Ncol 3705 FBAIN xL12 C 1 98907 EcoRI 3982 9 y a 82021 MELO Pe FIG 17D Ncol 1 FBA In BomHi 322 M DAGAT Ncol 1309 Nott 1582 Ura3 Pex20 3 ColE1 18B 2008 520193 A 2008 6 19 202 5882 ACTH lt 982 1980 da 66 821 a 61 652 opt mien le 194 996 vl BIE dq 806 Col EFN 99 696 21 521621 1 1 t 1 1 1 1 i i 1 i 1 i
127. WHIPS HOMNNL ZZ DAE SEI e a T CY ACU AL 27 093 50 2008 520193 A 2008 6 19 189 10 20 30 40 50 190 2008 520193 A 2008 6 19
128. 175 2008 520193 A 2008 6 19 DUDDD E nom D U U C C C C 10 20 30 40 50 176 2008 520193 A 2008 6 19
129. A5 11 1335 bp 12 444 AA 13 1335 bp 12 444 AA A5 A5 A6 Drd6 d5 V 14 1590 bp 15 444 AA AF309556 AS A6 16 1946 bp BC068224 A5 A6 17 1335 bp 18 444 AA Drd6 d5 M A17 19 1077 bp 20 358 AA 21 1077 bp 20 358 AA A17 C szo 22 957 bp 23 318 AA 24 957 bp 23 318 AA Cazo Cazo
130. 19707 4 04 YARROWIALIPOLYTICA 520000 TE oim Cea Sane OMA HHHHHHHHHHHHHHHHHHHHHHHe m 000000000000000000000000 HHHHHHHHHHHHHHHHHHHHHHH 0 NN HHHHHHHHHHHHHHHHHHHHHHHHH E HHHHHHHHHHHHHHHHHHHHHHHUH o _ m 1000000000000000000000000 CE I000000000000000000000000 A z m HHHHHHHHHHHHHHHHHHHHHHHHH n HHHHHHHHHHHHHHHHHHHHHHHHH sere ala HHHHHHHHHHHHHHHHHHHHHHHHH 000000000000000000000 2008 520193 A 2008 6 19 2 10 20 30 40 50 1 1
131. 2008 520193 A 2008 6 19 20 10 n 20 30 40 n 000 50
132. AF054508 Cre P1 Cre X03453 e XPR Xpr M17741 100bp 3 10 20 30 40 50 2008 520193 A 2008 6 19 136 10 20 30 40 C C C 137 2008 520193 2008 6 19 33
133. Mg d12 M M grisea 2 375 MG01985 1 2005 047485 A12 40 41 Nc d12 AABX01000374 20057047485 A12 F 42 43 Fg d12 1 233 20057047485 10 20 30 40 49 2008 520193 2008 6 19 A12 AB020033 Mad12 FILET A12 BAD08375 Skd12 A12 Kld12p LaBRI K Kll
134. Pex16 U75433 Pex16 2592 4684 GPAT DYE Lip1 GPAT GPAT 216 D9E galbana A9 71 1101 Lip1 Z50020 Lip1 4714 4747 LoxP 382 4761 6378 Ura3 AJ306421 6380 6413 LoxP 382 6470 7253 Ura3 AJ306421 784bp 5 9965 10480 Ura3 AJ306421 516bp
135. X69988 BsiWI EcoRI GPAT 1909 Pex20 929 14447 GPAT GPAT 216 lgD9e 1 galbana A9 71 Pex20 Pex20 Pex20 AF054613 EcoRI Swal TEF 1909 Lip1 14447 12912 TEF TEF AF054508 lgD9e 71 Lip1 Lip1 Lip1 Z50020 Swal Pacl FBAIN D8SF Pex16 12912 10325 FBAIN FBAIN 214 e D8SF A8 81 AF139720 Pex16 Pex16 Pex16 U75433 EcoRV BsiWI W497L AHAS Pmel 243 31957 6152
136. C 2008 520193 A 2008 6 19 36 10 20 30 DU 1 1 40 1 50
137. GPDIN EL1S Lip2 8078 10555 GPDIN GPDIN 211 e ELIS 1 24 AX464731 e Lip2 2 Lip2 AJ012632 EcoRI Clal Ura3 AJ306421 6450 8078 Pacl EcoRI TEF F A12 Pex16 4294 6450 e TEF TEF AFO54508 e F A12 A12 32 e Pex16 Pex16 Pex16 U75433 1 C U LL
138. FBAIN 214 973 bp FBAINm 215 924 bp 3 216 GPAT 1130 bp 217 YAT1 EF1 a TEF 436 bp GPM FBA 219 GPM GPD 220 GPDIN 221 EXP1 1000 bp 000000 D LT T HHHHHH Ug 2 pY5 30 6A 154 8 953bp pKUNF12T6E 8A 156 12 649bp pDMW271 8B 157 13 034bp pZP3L37 8C 158 12 690bp pZKUT16 8D 159 5 833bp
139. AF054613 TEF A6S Lip1 TEF TEF 20 AF054508 A6S A6 3 AF465281 Lip1 Lip1 Lip1 Z50020 Pmel Clal 4207 1459 Clal Pacl 1459 51 FBA F A12 Lip2 FBA FBA 213 F A12 A12 32 Lip2 Lip2 Lip2 39 AJ012632 TEF EL2S XPR TEF TEF AF054508 EL2S 27 6 677 145 XPR Xpr M17741 100bp 3 40
140. Y2031 YU TA ji Y2031 16 0 16 1 18 0 18 1 18 2 GLA pZUF6S 1 154 138 25 341 168 83 15 2 128 30 365 164 83 15 1 122 32 365 171 85 pZUF6S 4 363 166 84 pZUF6S 5 374 18 0 87 pZUF6S 6 376 187 86 pZUF6S 7 3188 36 0 17 8 9 1 25 14 9 126 29 35 9 17 3 8 8 15 0 121 32 36 3 173 86 pZUF6TYE2 1 424 168 97 pZUFGTYE 2 2 40 8 186 98 135 81 46 392 19 0 106 134 74 57 399 187 98 pZUF6TYE2 5 134 84 55 452 143 76 134 74 55 393 192 105 38 134 86 44 406 179 99 pZUF6TYE2 8 132 75 54 412 180 97 133 80 50 411 178 97 U 40 Ae A eat a ID00Ooooooooooooooo0o0o0oo0o0ooonnnono0o000 50
141. A 00000 ILE mg d d 00000 E 3 RT ELS A A A 00000 0 0 0 HO HB Bd 10 20 30 40 50 172 2008 520193 A 2008 6 19 C C C r1 10 20 30 173 2008 520193 A 2008 6 19 51 pZUF6S 197 197
142. Leu2 AF260230 2820 2109 M703bpO3 7 Swal BsiWI FBAIN 5 Pex20 8960 6315 e FBAIN FBAIN 214 MAAS A5 6 AF067654 e Pex20 Pex20 Pex20 AF054613 Swal Clal TEF MAA5 Lip1 8960 11055 9 TEF TEF AF054508 MAA5 6 Lip1 Lip 1 Lip1 Z50020 Pmel Clal Ura3 AJ306421 12690 11055 Clal Pacl TEF HA5S Pex16 172109 e TEF TEF AF054508 e HAS5S A5 13 NP_037534 e Pex16 Pex16 Pex16 U75433 HD Py D EE E 0
143. FBAIN 5 Pex20 Swal FBAIN FBAIN 214 8277 5632 MAAS A5 6 30 AF067654 Pex20 Pex20 Pex20 AF054613 Pmel GPM FBAIN 1 A5S Oct EcoRI Swal GPM FBAIN GPM FBAIN 219 10876 8278 1 A5S A5 10 2002 081668 OCT OCT OCT X69988 EcoRI Pmel Ura3 AJ306421 10876 12497 Pmel Clal YAT D17S Lip2 40 12497 14651 YAT YAT1 217 A17S S A 17 21 Lip2 2 Lip2 AJ012632 Clal Pacl GPD D17S Pex16 14651 1
144. 05 2 sszw zoxd 7ETEdZd el i i 243 910429 5371 vs VS 24 4 gs gt 85 49 VIS 6804 LE02A F 1 29 ZINA Nm f18606A 28 szeMWad premnad 668109102929 4 pysodzazd L 9 i rL vio se BBIZA VOCEA 9 08 9 ezAdivsAd 00051 bead BzAd BAd BzAd S8Ad 6 d ZAG 93 AE Y vv 91 St 2011 867 194 2008 520193 A 2008 6 19 FBAIN GUS FBA GUS GPD GUS GPM GUS TEF GUS 4256 FIG 6A FIG 6B 2000 9 1500 Hg 1000 i SD pu a 24 0 HG 88 P 24 HG 120 1 STEF mRNA Pmel 12690 Clal 1 89111 12672 Paci 2109 Leu 3 Sph 2820 PDMW271 13034 bp Pex20 3 BsiWI 6315 8B 195 2008 520193 2008 6 19 Uro3 516 Sph 12128 Lip2 3 pKUNF 12T6E 12649 bp 2 lt Fboj
145. A8 77 78 11 166993 A5 AF067654 6 7 6 075 183 A5 02 081668 A2 8 9 A5 NP_037534 11 12 A5 A6 AF309556 14 15 A5 A6 BC068224 16 A57A6 78 10 20 48 2008 520193 A 2008 6 19 A17 19 20 2003 0196217 A1 A4 AAN75707 104 105 Czozz AY591336 100 101 Cers 94 95 YE2 URUTAZ Cera TU
146. Pex20 Pex20 Pex20 AF054613 C C 0 10 20 30 40 186 150 42 pDMW302T16 186
147. a El ol 2 o lt a a a lt lt a ol ol 6 2008 520193 A 2008 6 19
148. pY86 179 179 RE 3399 4207 Lip1 Z50020 809bp 5 10 6919 7681 Lip1 Z50020 763bp 3 28 61 LoxP 382 63 1681 12 Ura3 AJ306421 1695 3358 TEF HPT XPR TEF TEF AF054508 e HPT Kaster K R Nucleic Acids Res 11 6895 6911 198344 e XPR Xpr M17741 20 100bp 3
149. 11 14 7 8 11 14 5 8 11 14 ALA 6 9 12 15 11 14 17 20 3w 3 EPA 5 8 11 14 17 20 5w 3 DPA 7 10 13 16 19 22 5w 3 DHA 4 7 10 13 16 19 22 6w 3 10 20 30 40 2008 520193 2008 6 19 30
150. AJ306421 784bp 10066 9275 5 Sphl Pacl Ura3 AJ306421 516bp 12774 1 3 Swal BsiWI FBAIN 0128 Pex20 6582 9275 e FBAIN FBAIN 214 e F A12 A 12 32 e Pex20 Pex20 Pex20 AF054613 Clal EcoRI pZP2C16M899 GPAT 6199 4123 IgD9E Pex20 EcoRI Swal pZP2C16M899 TEF 4123 2588 IgD9E Lip1 Swal Pacl pZP2C16M899 FBAIN 2588 1 385 Pex16 C 10 20 30 40 50 130 2008 520193 A 2008 6 19 um HHHHHHHHHHHHHHHHHHHHHHHH E HHHHHHHHH
151. IAR agit 62011 9 Not 1405 15825 i 5 Ura3 516 Lip2 3 DKUNF mkF 2 6191 1147 7145 bp BsiWI 1804 SphI 5303 qn 8 4 4 2 Uro5 764 5 8 x 5011 2385 Ascl 2595 FIG 10E amanet DE n nn MKSKRQAL PLTIDGTTYDVSAWVNFHPGGABIIENYOGRDATDAFMVMHSQBAFDKLKR MKSKROAL PLTIDGTTYDVSAWVNFHPGGAEIIENYQGRDATDAFMVMHSQEAFDKLKR MKSKROALSPLOLMEQTYDV VNFHPGGAEIIENYQGRDATDAFMVMHFQEAFDKLKR MKSKRQALSPLOLMEQTYDVSAWVNFHPGGAETIENYOGRDATDAFMVMHFQEAPDKLKR PO n MPKINPS SELPPQAAVNEAQEDFRKLREELIATGMFDAS PLWYSYKISTTLGLGVLGYFL MPKINPSSELPPQAAVNEAQEDFRKLREELIATGMFDASPLWYSYKISTTLGLGVLGYFI MPKINPSFELPPQAAVNEAQEDFRKLREELIATGMFDASPLWYSYKISTTLGLGVLGYFL Ad 44 74 MPKINPSFELPPQAAVNEAQEDFRKLREELIATGMFDASPLWYSYKISTTLGLGVLGYPL DE m e 78 FIGAVLLGMHY QQMGWLSHD I CHHOTFKNRNWNNLVGLVFGNGLOGFSVTUWK AUS MVOYOMYFIGAVLLGMHYQOMGWLSHDICHHQTFKNRNWNNLVGLVFGNGLOQGFSVTWWK 73 MVQYOMYFXGAVLLGMHYQOMGWLSHDICHHOTFKNRNWNNLVGLVFGNGLOGFSVTCWK RIS 74 MVOYOMYFIGAVLLGMHYQOMGWLSHDICHHQTFKNRNWNNLVGLVEGNGLOGFSVTCWK I I ROGO OR BOO DRHNAHBHSATNVQGHDPDIDNLPLLAWSEDDVTRASPISRKLIOFQOYYFLVICILLRET DRHNAHHSATNVOGHDPDIDNLPLLAWSEDDVTRASPISRKLIQFQQOYYFLVICILLRFI DRHNAHHSATNVQGHDPDIDNLPPLAWSEDDVTRASPISRKLIQFQQYYFLVICILLRFI 7 DRHNAHHSATNVOGHDPDIDNLPPLAWSEDDVT
152. 2008 520193 2008 6 19 RE BsiWI Ascl 1 Z50020 817bp 1 817 SA Sphl Pacl 1 Z50020 769bp 3525 4294 3 EcoRI BsiWI TEF rELO2S Pex20 13328 1 e TEF TEF AFO054508 e ELO2S IELO2 85 AB071986 Pex20 Pex20 Pex20 AF054613 Bglll EcoRI FBAIN D6S Lip1 10599 13306 FBAIN FBAIN 214 e 65 A6 3 AF465281 Lip1 Lip1 Lip1 Z50020 Clal Pmel
153. 37 142 153 Afd12p 62 105 116 Mad15 66 117 128 Skd15 58 119 130 415 52 101 112 Fm d15 56 95 106 Mg d15 54 88 99 An d15 60 101 112 Fg d15 64 117 128 Kld15p 65 130 141 Cad15p 67 132 143 Dhd15p 68 94 105 Afd15p HHHHHH utu 000 A 000000000 utu A 0000 A utu D A HHHHHHHHHHHHHHHHHHH ooo 0001000 A 10 20 30 40 50 54 2008 520193 A 2008 6 19 Ww 1
154. BsiWI 10957 Pmei 1811 Pex16 3 Pact 10345 TBA PDX3 5 7 Z D17DS 8911 3228 BsiWl 3618 Lip2 3 Clol 4233 pZP3L 37 12690 bp 6813 POX3 5 87 BsiWI 16043 HindllI 1 YLDI2 N ELISA oa Ascl 728 Swal 10475 P BsiWI 10437 Pex20 3 5 12663 bp Sphl 3436 FBAIN Swal 7942 SN 4 YL12 C 80111 7920 38 Pmel 7906 EcoRi 3992 EF FIG 8E Pex20 3 1501 819 A 5891 13300 Amp Lip 3 Sall 126821 Sphl 3527 15396Dp Lipt 3N FBAIN 5 Bglll 70602 1 A Pmel 10558 6i F D12 L1S Sall 18884 Lipa EcoRI 6453 Sall 8072 FIG 9A 1 1 Ncol 01 907 EcoRI 6947 22 Pex20 3 BsiWI 1224 59 Qu PocI 5436 5416 Sphl 5366 gt 4818 7502 5 ARS 1B 98 EcoRI 4020 Hindll 4008 FIG 90 EcoRI 10640 EcoRI 10618 FBAIN HindIII 572 Ncol 755 QtE2S Not 8689 DCT 5 1483 045 Ball 2216 Noti 2303 pZP2FOEN4 10660 bp Pex16 3 2648 POX2 3 Uro3 Sphl 3305 POX2 HindIII 6157 JP 2008 520193 A 2008 6 19 Bgl tt 7186 Ncol 6769 KpnI 6
155. AJ012632 EcoRI Clal Ura3 AJ306421 6453 8081 Pacl EcoRI TEF F A12 Pex16 4297 6453 e TEF TEF AF054508 e F A12 A12 32 e Pex16 Pex16 Pex16 U75433 LJ 1 1 L LJ 1 1
156. C 10 20 30 40 50 2008 520193 A 2008 6 19 156 10 C C 20 C C 30 40 DU C 50 C C
157. Hindlll 238 ATG A 1 HHHHHH HHHHH 00 0000 1000 HHHHHH C E cde a lt Eas C C C n Ru 10 20 30 40 50 92 2008 520193 A 2008 6 19 I C C C CH C H A H C 2 EE
158. EXP1 1000 1bp 6 221 SLO ATC BIRSU OFA IDEE on ENB FBAINm FBAIN FBAINm FBAIN ATG 52bp N 22 FBAIN FBAINm 10 20 30 40 50 2008 520193 A 2008 6 19 68 10 20 30 40 50 1 1
159. C 2008 520193 A 2008 6 19 89 10 20 30 40 50 1 H C n C H C
160. Y2107U1 ARA ETA EPA 28 04 17 4 Y2107U1 pMGPAT 17 1 3 2 14 8 11 7 298 5 6 2 0 0 3 184 04 16 9 Y2107U1 pMGPAT 17 3 14 3 10 8 27 5 72 17 4 Y2107U1 pMGPAT 17 4 15 7 11 5 29 1 6 3 0 4 17 3 i Hen a B B B 10111 m u B n n a B H HHHHHHHHHHHHHHHHHHHHHHHHHHHH HH HH HHHHHHHHHHHHHHHH HHHHHHHHHHH IHHHHHHHHHHHHHH HHHHHHHHHHH HHHHHHHHHHHHHHHHHHHHHHHHHHHH IHHHH UU OU 000000 A A 00000 004000 A nnnnnHHHHHHHHHHHHDH 000 0000000000 0 0000000000000 U 0 HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH IHHHHHHHHHHHHHHHHHHHHHHHHHHHHH HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
161. Pex16 U75433 EcoRV FBAIN OtE2S Oct EcoRI Swal e FBAIN FBAIN 214 10618 8345 OtE2S OtE2S 102 AY591336 e OCT OCT OCT X69988 Spel Spel Xbal Ura3 AJ306421 12582 11095 000000000000000000000000000000000 D00000000000000000000000000000000000000 IH CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH 10 20 30 40 50 114 2008 520193 2008 6 19
162. 159 RE BsiWI Pacl Ura3 AJ306421 1 721 721bp 5 Sall Clal Ura3 AJ306421 3565 4289 724bp 3 Clal BsiWI TEF rELO2S Pex20 4289 1 e TEF TEF AF054508 ELO2S IELO2 85 AB071986 e Pex20 Pex20 Pex20 AF054613 0 CH 10 20 30 40 50 2008 520193 A 2008 6 19 108 10 20
163. 210 e D8SF A8 81 AF139720 Pex16 Pex16 Pex16 U75433 Salll Pmel Leu2 AF260230 4897 2417 40 C 2 C 50 2008 520193 A 2008 6 19 133 10 20 30 40 A
164. 3359 3392 LoxP 382 Pacl Fsel FBAIN FmD12 Lip2 7690 7 FBAIN FBAIN 071 214 FmD12 A 12 32 e Lip2 Lip2 AJ012632 Lip2 30 a H w yaa VENE 8 CAU DP H H 40 i U U 000000000 0000000 000000000000000000000 138 2008 520193 A 2008 6 19 34 pY94 180 180 RE Pacl Swal FBAIN D8 Pex16 1 2587 FBAIN FBAIN 214 08 AB8 81 AF139720 Pex16
165. ELO3 Y2031 Y YRUF 772031 pZUF6S 1 12 382 198 69 pZUF6S 2 HEE 10 1 234 390 175 74 pZUF6S 43 39 0 202 7 0 pZUF6S 4 5 24 1 40 8 198 69 pZUF6S 5 22 4 pZUF6S 6 22 7 pZUF6S E3WT 3 1 17 3 pZUF6S E3WT 3 2 36 9 213 11 5 pZUF6S E3WT 3 3 8 9 183 33 8 354 0 0 pZUF6S E3WT 3 6 19 9 A 378 174 123 pZUF6S E3WT 5 1 86 17 6 376 211 11 1 pZUF6S E3WT 45 2 88 174 376 213 112 pZUF6S E3WT 45 3 91 171 376 215 114 pZUF6S E3WT 5 4 17 9 38 0 19 3 11 7 pZUF6S E3WT 5 5 x 16 1 37 0 21 6 11 7 20 65 5 6 8 7 21 5 pZUF6S E3WT 6 1 16 9 pZUF6S E3WT 6 2 16 2 pZUF6S E3WT 6 3 36 2 218 11 3 pZUF6S E3WT 6 4 8 3 166 36 9 219 122 pZUF6S E3WT 6 5 5 360 178 13 4 pZUF6S E3WT 6 6 87 195 354 181 132 pZUF6S E3WT 7 1 0 0 355 182 158 pZUF6S E3WT 7 2 80 177 40 377 209 117 pZUF6S E3WT 17 3 42 360 214 117 pZUF6S E3WT 7 4 0 0 281 43 370 169 13 6 pZUF6S E3WT 7 5 47 367 212 124 pZUF6S E3WT 7 6 80 180 48 363 208 121
166. 1 1 5 84 1949 58 Sile 684 SES 91 9861 0681 m ANG GI F lt 18C 1 44 GZZ Sr SEKU 7 46 dq ZIZD Or eea 1 vy 914 dq 1612 ore 8 2 1 or EcoRV 3110 T um Sm 8 gt amp 5 lt LL 58 5 S Tu eco lt e S z SS a lt ol rt 122 58 m 5 mE S 06 441290 26 YNGI 94 ZGGE 16 ERIEN 1 1 1 1 da cZZZ 06 8 6 YNG 6 dq 001 68 tusa dq 209 89 amp EON VV 6 2 dq 828 8 98 lod E 2013 1 1 i 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 dq 6607 6 SBN 098 616 0 1 8961 le Bam 10334 ColE1 Ncoi 9670 FBAIN EcoRI 8647 EcoR pZUF6S E3WT 11046 bp HindIII 3102 EcoRV 3110 EcoRI 3114 EcoRV 3590 Hind HI 3912 Sphl 44601 Spel 4725 B 218 56981 1512 EcoRi 1534 FBAIN pZUFGF YE1 1
167. 2008 520193 2008 6 19 19 JP A 11 2008 520193 P2008 520193A 43 2 6 19 2008 6 19 61 Int Cl C12N 1 19 2006 01 C12N 1 19 ZNA 2B005 12 1 15 2006 01 C12N 1 15 2B150 C12N 1 21 2006 01 Cl2N 1 21 45001 C12N 5 10 2006 01 C12N 5 00 A 4B018 C12P 7 64 2006 01 61 2 7 64 ABO24 BARR RK PRB RRS 200 R BRACES 21 2007 540127 P2007 540127 71 390023674 86 22 17 11 3 2005 11 3 85 19 6 29 2007 6 29 86 PCT US2005 040256 E 1 DU PONT DE NEMO 87 W02006 052871 URS AND COMPANY 87 18 5 18 2006 5 18 31 60 624 812 100 32 16 11 4 2004 11 3 7 33 US 74 110000741 74 100060782 72
168. 70 263 AA 9 A8 72 1275 bp 73 419 AA AAD45877 A8 74 422 AA Wallis Archives of Biochem Biophys 365 307 316 19994 00 34439 75 1270 bp 48 D8S 1 76 1269 bp x A48 D8S 3 A8 Eg5 77 1271 bp 78 421 AA A8 Eg12 79 1271 bp 80 421 AA 81 1272 bp 82 422 AA A8 D8SF C gis 83 2628 bp 84 267 AA
169. YE2 8857 415 e TEF TEF AF054508 YE2 YE2 74 CAG77901 Aco3 AJ001301 L1 L3 L3 ooo L1 L3 L3 5 1 0O E oaoa 174 2008 520193 A 2008 6 19 Es E 53 pZUF6S pZUF6TYE2
170. 1000 11 GUS XPR RE GPD YL211 YL212 968bp gpd Sall pYZGDG 225 ATGJ Ncol 226 210 GPDIN YL376 YL377 qgpd 973bp Pstl Ncol pDMW222 227 lt 201bp 228 49bp 194bp 146bp Pstl Sall 211 GPM YL203 YL204 875bp gpm Ncol pYZGMG 229 ATG 212 GPAT 1130bp lt gpat Sall pYGPAT ATG Ncol GUS 216 FBA fba Ncol pDMW212 1001bp 1bp Sall 213 FBAIN fba 804bp Ncol pDMW214 169bp Sall 235 62bp 165bp 236 102bp 214 YAT1 27203 F yat1 Hindlll pYAT GUS 27203 R 778bp 1bp Sall Ncol 237 217
171. AJ306421 5933 13 516bp 3 EcoRI BsiWI 12 20 6380 8629 TEF TEF AF054508 Y A12 A 12 28 20 Pex20 Pex20 AF054613 40 2008 520193 A 2008 6 19 115 10 20 30 1 40 n 50
172. pKO2UM25E 8E 160 12 663bp pDMW303 9A 161 15 996bp pZUF17 9B 162 8 165bp pZUF4 163 8 633bp pZUF4S 9C 164 8 633bp pZP2FOEN4 9E 165 10 660bp pKUNT2 10A 166 6 457bp 10 20 30 40 19 pDMW237 167 7 879bp pYS4PC 168 8 502bp pKUNFmkF2 10E 169 7 145bp pZF5T PPC 13B 170 5 553bp pDMW297 13D 171 10 448bp 2 2 16 899 14 172 15 543bp pDMW314 14B 173 13 295bp JP 2008 520193 A 2008 6 19 pDMW325 14C 174 15 559bp 10 176 10 189bp 177 12 558bp 178 8 982bp pY86 15D 179 10 424bp 94 16A 180 10 485bp pY91M 16B 181 8 423bp pDMW232 16C 182 10 945bp 183 8 194bp 184 10 838bp 20 185 6 912bp pDMW302T16 186 14 864bp pZKUGPE1S 187 6 540bp pZKUM 189 4 313bp PMLPATAn 191 8 414bp 192 7 323bp pMDGATt17 pMDGAT1 17 193 8 666bp SDE 17 194 8 084bp pZF5T PPC E3 196 5 031bp pZUF6S E3WT 21B 198 11 046bp PZK GPYE2 21D 200 6 498bp pZKUGPYE1 202 6 561bp 203 10 809bp pYCPT1 17 204 8 273bp pZP2I7 Ura 22C 205 7 822bp 40 1 2 217 206 7 930bp pTEF ISC1 7 207 8 179bp 208 8 196bp 209 5 105bp HHHHH HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH 0000000000000000 I10000 IHHHHHHHHHHAHHHHHAHHH IHHHHHHHHH HHH 50
173. 10 20 30 40 50 2008 520193 A 2008 6 19 167 10 20 30 40 50 2008 520193 A 2008 6 19 168
174. 110957 1 GPAT EL1S XPR e GPAT GPAT 216 EMIS 1 24 AX464731 e XPR Xpr M17741 100bp 3 Bglll BsiWI 8578 11095 Sall Pacl 6704 8202 FBAIN M A12 Pex20 e FBAIN FBAIN 214 M A12 A12 AF417245 30 Pex20 Pex20 Pex20 AF054613 Ura3 AJ306421 EcoRI Sall 3992 6704 FBAIN M A6B Pex20 e TEF 054508 A6 B AB070555 4 e Pex20 Pex20 Pex20 AFO054613
175. 3 Clal BsiWl TEF 175 Pex20 4233 6043 e TEF TEF AF054508 A17S S A17 21 2003 0196217 A1 e Pex20 Pex20 Pex20 AF054613 Clal Pmel FBAIN A17S Lip2 4233 1811 e FBAIN FBAIN 214 e AA17S 21 e Lip2 Lip2 Lip2 AJ012632 Pmel Swal Leu2 AF260230 1811 1 Pacl Swal FBAINm A17S Pex16 10345 1 e FBAINm FBAINm 215 e AA17S 21 e Pex16 Pex16 Pex16 U75433 0000 10 20 30 40 50 2008 520193 2008 6 19
176. 7920 10437 e FBAIN FBAIN 214 MA12 A12 AF417245 30 e Pex20 Pex20 Pex20 AF054613 Sall Pacl Ura3 AJ306421 6046 7544 EcoRI Sall TEF 1 A5S Pex20 3992 6046 9 TEF TEF AF054508 e 1A5S A5 10 2002 081668 e Pex20 Pex20 Pex20 AF054613 2008 520193 A 2008 6 19 HHHHHHHHHHHHHHHHHHH HHHHHHHHHHHHHHHHHHHH I0000000000000000000 I s IHHHHHHH HHHHHHHHHHHHHHHHHHHHHHH 0000000000000
177. Sphl Pacl 1 Z50020 35278 4297 769bp 3 Swal BsiWI GPAT HA5S Pex20 133001 GPAT GPAT 216 HA5S A5 13 NP_037534 Pex20 Pex20 Pex20 AFO054613 Bglll Swal FBAIN D6S Lip1 10602 13300 e FBAIN FBAIN 214 65 A6 3 AF465281 Lip1 Lip1 Lip1 Z50020 Clal Pmel GPDIN EL1S Lip2 8081 10558 e GPDIN GPDIN 211 e EL1S 1 24 lt AX464731 Lip2 2 Lip2
178. i 10 20 30 40 50 122 2008 520193 A 2008 6 19 A i 8 20 40 123 2008 520193 A 2008 6 19 12 8 1 Eg12 12 8 2 Eg12 12 9 1 Eg12 O O N O Co Ajo 23 A 8 S EDA 16 0 46 1 18 0 48 1 20 2 20 5 20 2 7 18 11 14 75 1 5 HST SALE ES 75 2 RERO 5 5 6 1 Eg5 6 6 2 24 5 6 2 Eg5 6 7
179. 01 1 16 1 18 2006 01 1 16 JUD 3606 2006 01 1 18 2006 01 1 18 2006 01 1 18 log 2524 2006 01 1 18 2006 01 35 72 300 2006 01 25 28 1 00 2006 01 25 32 25 24 25 20 3 00 11 00 80000 AP BG GM KE LS SD SL SZ TZ UG AW AZ BY KG KZ M RJ TJ EP AT BE BG CH CY CZ ES FI FR R HJ LE IS LT LT LU LV MC N PL PT RO SE 9 SK TR OX BF B CF GG MG N GQ M NE SN TD AG AL AM AT AU AZ BB BG ER BWBY EZ CA CH CN 09 CR QJ CZ DM I EC EG ES FI DE H GYR HJ ID IL IN TS J P KE KG KM KN KR KZ LG LK L R LS LT LU LV LY M MG MK MY MVMK MZ NA NG NO NZ OM PG PH PL PT RO RU SG SD SE SG SK SL SM SY J TMTN TR TT TZ UA UG UZ VG W YU ZA ZM ZW 72 72 72 72 72 72 72 72 72
180. 2 19 12 8 1 Eg12 6 9 1 7 12 8 2 Eg12 5 8 1 8 12 9 1 Eg12 5 NE M 12 9 2 Eg12 5 8 HHHH l 3524 A 8 S ETrA ass 20 3 20 4 20 3 eniam 75 1 5 75 20 5 1 5 5 6 1 Eg5 ee 7 32 5 6 2 Eg5 6 m 12 9 2 Eg12 6 35 20 2 9 000 0001000 HHHHHHHHHHHHHHHHHHH HHHHHHHHHHHHHHHHHH A z UUU U U HHHHHHHHHHHHHHHHHHHHHHHHHHHHHH HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH 10 20 30 40 50 124 2008 520193 A 2008 6 19
181. 2008 6 19 80 1 1 000 IUUUUU 10 20 30 40 50 2008 520193 A 2008 6 19 81 10 20 30 40 50 n
182. 30 40 50 C 2008 520193 A 2008 6 19 120 10 20 30 40 50 C C
183. 343 85181 400 Ura5 784 am 08 8011 6060 EcoRI 5620 Ascl 1191 FBAINmK 2 Swal 4819 7013 bp 80111 4797 Pme 4783 Clol 4436 Pacl 4421 Ura3 516 Sphl 3899 Pex16 3 346 89111 4520 Nco 420214 pDMW287F 5413 bp Clol 3230 Pmel 3199 EcoRI 9053 8911 1291 Hindlll 9041 D8SF f1 ori pDMW297 10448 bp ColE1 Bsiwl 6257 5911 4082 EcoR 4101 20 3 5 4 EcoRI 4123 Not 5940 13D pDMW314 13295 Fbot Swol 6582 Pmel 6546 14B D17 Clal 14651 3 Paci 1426 BsiWI 14036 J 175 YAT Sphl 2137 Pmel 12497 Ura3 pDMW325 EcoRI 10876 15559 bp GPM FBA Fool 14C 199 2008 520193 A 2008 6 19 PAT Bsiwi 929 Clal 980 Cial 1052 c ae 1 BA 1 FBAIN pZP2C16M866 08 15543 5 Pex16 3 Pacl 10325 POX2 3 BsiWl 6152 POX2 Asc 6962 14A Sphl 9670 pZKSL5598 16325 bp FIG 14D Pacl 1 Lip1
184. 520193 A 2008 6 19 538 Q d12D Drd6 d5 M Drd6 d5 V 3 lt w 6 pY102 V LA pY102 V ALA pY102 M EDA pY102 M DGLA pY102 V DGLA pY102 M pY102 V POO L1 L3 L3 L3 L3 2008 520193 2008 6 19 144 000 10 W A
185. 000 D A 0000 AQ 0 0 AQ A uu A 00000 0 0 00000 1 1 l1 Dl U 10 20 30 40 50 47 2008 520193 A 2008 6 19 5 DHA ORF A6 AF465281 12 FILET 5 968 809 A6 AB070555 4 5 FIEF Cszo AX464731 22 23 ELO1 FRET 00 12720 Cszo 6 677 145 25 26 ELO2 A9 AF390174 69 70
186. 0000 0000 000000 000 0000000 000 10 20 30 40 177 2008 520193 A 2008 6 19 55 pZUF6S pZUF6FYE1 Y2031 16 0 16 1 18 1 18 2 GLA LPZUF6S 1 28 129 182 10 7 PZUFGSSQGHE 126 186 10 3 LpZUF6S 3 130 178 10 6 2 65 4 13 1 489 10 3 pZUF6S 5 10 9 pZUF6S 6 10 4 10 5 pZUF6FYE1 1 174 219 16 9 pZUF6FYE1 2 167 228 16 1 pZUFGFYE1 3 15 8 pZUF6FYE1 4 16 8 DPZUF6FYE1 5 17 2 17 7 21 9 21 9 17 9 16 5 i a C Ci
187. 00000000000000000000000 D000000000000000000000000000000000000000 20 000000000000000000000000000 DOOO0OO0OOO0O000000000000000000000000 zi C 30 ooog w 40 2008 520193 A 2008 6 19 141 9oN L3 96 0 10 16 CN m 238 9 Inf 80 2 712 L Lt LLC OG WI6AG S EET 40 OLZ OG ALGAG SL L1 2 LLZ OG WLGAG SLIT L L ax BHEX S117 LLA OG WIL6AG S 11 UU 96c 06 VIO 9068 1 81 960 8L L9L 0 9L 4 y COL E BR COO XES V V VEI LAY 2 TAE LC VIRES 6 FWNL6A 50 HHHHHH 2008 520193 2008 6 19 142 10 20 30 8 911 LLZ OG NL6AG SL 17 BHIX SLL7 LLZ OG NL6AG SL 1 LEE 40 50 143 2008
188. 008 6 19 164 5 GTAATACGACTCACTATAGGGCACGCGTGGTCGACGGCCCGGGCTGGT 3 3 H2N CCCGACCA 5 10 H H 1 1 20 lt lt lt 30 40 50 0 0 165 2008 520193 A 2008 6 19
189. 0809 bp 065 Sall 3648 20 3 8 111 1176 D17st Sat 1556 6329 BsiWI 1566 POX2 3 sphI 15674 pZP217 ura 1822 bp Pex20 3 85181 2156 POX2 88 AsiS1 2944 Ascl 2966 203 2008 520193 A 2008 6 19 pZKUGPYE2 6498 bp FIG 21D 00000 20 BsiWi 1 Not 6245 Ura3 3 Cial 5951 Poci 721 Ncol 5766 YE1 EcoRI 5284 Ncol 5264 pZKUGPYE1 6561 bp GPAT PstI 3912 Sall 3565 FIG 22 EcoRI 6528 PstI 6527 Pstl 6516 EcoRI 6506 EcoRI 5543 Hindli 3706 FIG 22D Ncoi 4633 PstI 4357 5 204 2008 520193 A 2008 6 19 10000 2008520193000001 xni 205 2008 520193 A 2008 6 19 511 01 10000 230 2006 01 0000 1 30 0 0000 15 09 2006 01 15 00 9007 2060 900 912 20601 9 152 30 2006 01 3 00 3 22 200601 202 416 2006
190. 1 1 lt 100 0 2008 520193 2008 6 19 103 10 C C 20 30 1 1 40 1
191. 1 8 0 00000000000000000000000000000000000000 1 1 11 11 1 111 11101 11 40 A A A 50 180 2008 520193 A 2008 6 19 C C 10
192. 11 1 HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH C HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH HHHHHHHHHHHHHHHHHHHHHHHHH HHHHHHHHHHH 10 20 30 40 50 106 2008 520193 A 2008 6 19 t IHHHHHHHHHHHHHHHH 0000000 HHHHHHHHHHHHHHHHHH IHHHHHHHHHHHHHHHH D00000000000000000 00000 10000000000000 10000000000000 0 00000 00000000000 HHHHHH X17 Z2A amp RFpZP3L37 BIBS 158 158 RE Ascl BsiW Pox3 AJ001301 6813 6043 763bp 5 Sphl Pacl Pox3 AJ001301 9521 10345 818bp
193. 20 30 40 50 181 2008 520193 2008 6 19 1 U 57 S ISC1 M4 16 1 18 0 18 1 GLA 13 5 87 218 89 09141 93 M4 pTEF ISC1 21 3 75 07 39 10 7 HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
194. 20 30 40 50 2008 520193 2008 6 19 41 10 20 30 40 50 ol ol 1 1 42 2008 520193 A 2008 6 19
195. 260230 703bp 8258 8967 3 Swal BsiWl FBAIN MAA5 Pex20 2114 4755 FBAIN FBAIN 214 e MAAS 5 6 AF067654 Pex20 Pex20 Pex20 AF054613 Swal Clal TEF MAA5 Lip1 2114 17 TEF TEF AF054508 MAA5 6 Lip1 Lip1 Lip1 Z50020 Pmel Clal Ura3 AJ306421 55507 4755 L L3 L3 E a Ed
196. 3N Foal pY12 210xp Hyg Fbo F15 10189 bp CoiE1 Ura3 Ascl 6713 EIE TN Clal 4194 LOXP TEF P HPT 4609 15A Swal 2417 Foal Bomii N N pY91 Dr D6M 8423 bp Bst11071 4040 Chal 4193 16B 200 2008 520193 A 2008 6 19 Loxp Sful 9627 Sall 1682 F D12 Clal 16913 TEF P Bam 2106 Lip2 3 HTP 7687 Lipt 3N Lip1 5 N Ascl 4210 BssHI 4210 ColE1 FIG 15D 5 1 516 rpacl ta 19952 Swa 2588 Lipt 3 pY94 Ioxp D9ED8 Uro3 10485 bp 1 9 09 16A Bgill 787 Lip1 Swol 2114 Foal pDMW232 094 P BglII 3443 MAD5 Pex20 3 nd mU 4755 16C Notl 1 7682 Ncol 189 Nco 7663 621 Not 1226 ECORI 1244 XPR2 we BsiWI 1517 pY3T F15 8134 bp ColE1 5729 Sphl 5659 FIG 160 Swal 1 FBAINm 561 93001 EcoRI 802 D12 act Pmel 8525 E amem 8163 10838 bp S Hindlll 2655 S YLD12 N Ura3 3382 Soll 6665 EcoR 6646 SphI 60901 FIG 17A
197. 40 2008 520193 A 2008 6 19 35 pY91M 181 181 RE 2866 4170 ARS18 A17608 4216 5703 Ura3 AJ306421 Sa Bsiwl FBAIN DrD6 Pex20 19 5705 8423 FBAIN FBAIN 214 e DrD6 A 5 A6 Drd6 d5 M 17 BC068224 Pex20 Pex20 AF054613 Pex20 000000000000000000000000000 0000
198. 424 EcoRI 5724 HindIII 2785 EcoRI 2797 Pex20 3 BsiW 1866 FIG 9C 13 Pmel 360 Ncoi 854 BsiWI 1061 012 Not 2117 20 3 BsiWl 2434 Uro5 784 Ascl 3225 5911 3015 FIG 10A 197 2008 520193 A 2008 6 19 TEF pex20 3 Clal 7968 BsiWI 318 EcoRI 7952 EcoRI 6989 Not 1264 LEU2 jos Pac 1483 pDMW240 EcoRI 6063 8380 Amp Sphl 57001 P ColE1 EcoRI 6041 Sall 6022 HindIII 5152 Uro 1 ori Hindlll 31021 a 45301 EcoRI 3114 Clal 4510 ARS 18 FIG 10C Sphl 4460 HindIII 39121 10B m NotI 1 13 59 A8 snes lt I Not 1519 555 1841 1 7507 2 gt GPD 23 LEU2 gis Ss 13 P 4 3222 gar E 8 1 E FESS Y PLAC 858 osa y 3 T l AS SG 5571 PuB1 LL AL BNP 5 8 pist 3o 2 N s lt LL Na p gt 5 2 are P 5 EcoRI 102404 FNcol 1
199. 426 GPD GPD 210 A17S 21 Pex16 Pex16 Pex16 U75433 50 2008 520193 A 2008 6 19 131 C 10 20 C 132 2008 520193 2008 6 19 IHHHH 29 pZKSL5598 175 175 RE Ascl BsiWl Lys5 M34929 794bp 10409 9573 5 Sphl Pacl ys5
200. 79 A15 ELIF AB182163 Mad15 PEF A15 XM_451551 Kld15p A15 EAL03493 Cad15p A15 BAD11952 66 Skd15 10 20 30 40 50 2008 520193 2008 6 19 A15 CAG88182 67 Dhd15p CBS767 A15 2 EAL85733 Afd15p SangerlInstitute University of Manchester Institute for Genomic Research TIGR A nidulans Center for G
201. 9 79 10 1 1 20 30 l 40 50 1 1 2008 520193 A
202. HHHHHHHHHHH HHHHHHHHHHHHHHHHHHHHHIH n n 000000 Hod HHHHHH 10 1 A 2528 20 pDMW325 174 174 RE Ascl BsiWl Leu2 AF260230 788bp 4837 5632 5 Sphl Pacl Leu2 AF260230 703bp 2137 1426 3 Pme BsiWI
203. HHHHHHHHHHHHH HHHHHHHHHHHHHH HHHHHHHHHHHHHHHHHHHH 8 DHA ORF 124 125 PILEF 11 024544 136 137 117024545 138 139 108 109 110 111 607689031 LPCAT TO06E8 1 121 320041076617 A2 D 1 L1 63 2008 520193 A 2008 6 19
204. LTqIT 86 87 ELO3 Ceis Sry AB071 986 83 84 rELO2 Ce 97 98 YE1 YRY FID A12 2004 104167 28 29 A12 A12 Fm d12 A12 An d12 A12 Aspergillus flavus AF417245 2005 047485 A 1 15 scaffold 1 AAG36933 2005 047485 AY280867 VERSION AY280867 1 gi 30721844 2005 047485 30 31 A12 Afd12p AFA 133c 344248 345586 AfA5C5 001c 2005 047485 A12
205. PT Kaster K R Nucleic Acids Res 11 6895 6911B 1983 Xpr M17741 100bp 3 5862 5895 LoxP 382 10 20 30 40 135 2008 520193 A 2008 6 19 31 pY80 177 177 RE Pacl Fsel WRESA CHSGPD FmD15 XPR 47 2375 e GPD GPD 210 e FmD15 A 15
206. RASPISRKLIQFQQYYFLVICILLRFI IEEE 7 WCEQSVLIVRSLKDRDNQFYRSOYKKEA IGLALHWILKTLFHLEFMPSILTSLLVFFVSE 180 NCFOSVLTVRSLKDRDNQFYRSQYKKEAIGLALHWTLKALFHLFFMPSILTSLLVFFVSE RUHI j 73 WCFQCVLTVRSLKDRDNQFYRSQYKKEAIGLALHWTLKALFHLFFMPSILTSLLVFFVSE 74 NCFOCVLTVRSLKDRDNOFYRSQYKKEAIGLALHWTLKALPHLFFMPSILTSLLVFFVSE 78 LVGGEGIAIVVEMNHYPLEKIGDSVWDGHGFSVGOIHETMNIRRGIITDWFFGGLNYOIE 180 LVGGFGIAIVVFMNHYPLEKIGDSVWDGHGFSVGOIHETMNIRRGIITDWFFGGLNYOIE 73 LVGGFGIAIVVEMNHYPLEKIGDPVUDGHGFSVGOIHETMNIRRGIYTDWEFGGLNYQIE 2 74 LVGGPGIALVVEMNHYPLEKIGDPVWDGHGFSVGQIHETMNIRRGI ITDWEFGGLNYQIE FEISS IO ISIE IGG RR RA RARE EREI ICO ITO OEE 178 HHLWPTLPRHNLTAVSYQVEQLCOKHNLPYRNPLPHEGLVILLRYLAVPARMAEKQPAGK 580 HHLWPTL PRANLTAVSYOVEQLCOKHNLPYRNPLPHEGLVILLRY LAVPARMAEKQPAGK 573 HHLWPTLPRANLTAVS YOVEQLCQKHNLPYRNPLPAEGLYILLRYLAVEARMAEKQPAGK AU 74 EBLEPTLPRHNDTAVSYOVEQLCORBNLPYRNPLPHEGLVILLRYLAVFARMAEKQPAGK 8 AL 180 AL fene 73 S74 12 2 5 5553bp FBA Clal 13230 Pmel 3199 f1 ori 13B 198 2008 520193 A 2008 6 19 Lip2 3 Clal
207. aOB 35614 36861 m GLV KLLA0B00473g ORF A12 EAK94955 Cad12p A12 CAG90237 Dhd12p CBS767 A15 2005 047479 Fm d15 A15 A An d15 1 122 scaffold 9 A15 Mg d15 2005 047479 M 2 1597 MG08474 1 2005 047479 A15 Nc d15 AABX01000577 20057047479 A15 Fg d15 F 1 320 BAA33772 1 2005 0474
208. av FERA 40 7 8 ra 2987 DBS ab gt gt 8 HAHA 47 a 0 OW 4 4 AL OA 26 VY Ban ow 4D PZR 1 250 1 ove 4t aa gre cnt ene IT D TIAE Lt 24 588 lt 2 803 ZUS V09 7 9 28 CHEM ein Pool 2 VOHRA lt lt 4 3 lt 1 2 WAGLLCL ELL GELY I d 6 9 38 us Ny 29 50 12208 8 1 LVd9 a CI LY read EAN Bir fe 4f 0afig us C Vy an pes 9 85 9r1nyzd Bazanzond i i you 615429 VAIS 5 9 H i ZNLOLZA QL902A 19024 Ya 01 Yad 0L vuv uo 3 3 6 928 lt 81946 2 aszwrizond 8 91 02 Z9E02 d 2 SA uU 721v Vda 8L i ae1zi AN Qd 68024 4 270 Sb AE LL VY LY 2202 CFT 09024 7 epoza CT 2902 9 i i 860ZA
209. enome Research CGR Cambridge MA M grisea CGR International Rice Blast Genome Consortium F graminearium CGR International Gibberella zeae Genome Consortium IGGR sa popup apaq o000000000 00 E E SEKE H HHHHHHHHHHHH 0000000 000000 es d NES SN oe A z Sya z N E ESS ysam a 5 SKE ae x A A A IHHHH HHHHHHH 000 000000 HHHHHHHAHHHHHHHH HHHHHH HH IHHHHHHHHHHHH
210. o 6A9 A8 oO 3A9 A8 A9 A8 A9 ELO A8D A5D A17D 62022 ELO A4D A15D A9 ELO A8D A5D 02022 ELO A4D A15D 9 ELO ABD A17D C2022 ELO A4D PUFA w 3 w 6 PUFA w 3 w 6 GLA D ELO 10 20 30 40 50 45 2008 520193 A 2008 6 19 Po gt t m L2 m
211. ol MC rina 4 lt 2 DE 9 50 3 erkek AV 19 9 020 8 WUE GAL 0 4 dide BORG YO2 Ne VoDr 1E3L fr azara svd A dAV d1V 32 V02 t lt tai x lev 0 2 z via vis 020 le o le 6 12 4 4 3 5 BOICOARRURT 4 WOO 5 9 9 0221 15 9 819 959 Bosh lt 623 a eczasov En GY 43 4 4 lt 4 70 em z 0zol Werk ev lopo 3 4 5 fog 5 3 0819 lt 5 gt Benccx YI AHAS W497L 5 YURMA Pl a SIPON glp uy SN xis pua xdg piy SLPYS LPYS pY57 Y AHAS W497L 3 9370 bp 0010 E x VE 2008 520193 A 2008 6 19 193 SAI LY 2 08 Od YE UE BE LNS us FE H 1 06 Od AETHER Zh 8 c
212. p 48 415 AA 15 2008 520193 A 2008 6 19 A12 49 436 AA CBS767A12 50 416 AA A15 51 1209 bp 52 402 AA A15 53 1206 bp 54 401 AA A15 55 1185 bp 56 394 AA A15 57 1290 bp 58 429 AA A15 59 1212 bp 60 403 AA A15 61 1353 bp 62 403 AA A15 63 1248 bp 64 415 AA A15 65 433 AA A15 66 419 AA CBS767A15 67 435 AA A15 68 396 AA A9
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