Freezing Medium for human ES/iPS Cells
Contents
1. ES iPS ES Medium ReproFF 5ng mL bFGF 15 ml 1 B1 15 mL ES Medium 10 mL 37C B2 B3 B1 B4 37 C ES Medium2. 15 15ml p 1000 P 1000 200 ux A2 ES iPS A3 ES Medium PBS 2 3 mL A4 1 mL 37C C0 5 A5 ES Medium 2 3 ml 1 5 mL A6 170Xg 1 000 rpm 4 5 A7
3. 1 1 A8 1 A9 p 1000 200 uxL 2 A10 A7 2 3 3 A10 15 A11 135C WWW reDrocell com 5 po 3 ReproCELL B ES Medium 1
4. Freezing Medium for human ES iPS Cells ReproFF ES iPS A 60mm 1 Freezing Medium for human ES iPS Cels
5. ReproCoat 1 t RCHEOT001 ReproCoat RCHEOTO002 003 bFGF RCHEOTO004 Lamimin 5 RCHEFCO001 Feeder Cells SL10 RCHEFC003 Feeder Cells MEF http www_reprocell com E mail info jp reprocel com WWW reDrocell com i
6. Primate ES Cell Medium 5ng mL bFGF RCHEOT002 003 ES Medium ReproFF 5ng mL bFGF Dissociation Solution for human ES iPS Cells ES iPS 35 mm 100 mm 1 1 PBS CaTMgT free PBS 15 ml p 1000 ml A1
7. po 3 ReproCELL Freezing Medium for human ES iPS Cells Cat RCHEFMO01 80 C 2 8C 2 3 5 135 PS Takahashi K et al Cel 131 861 72 2007 PH Ready to use DMSO0 Propylene glycol 2 Mercaptoethanol
8. 800 L 2 3 B5 B1 15 mL B6 170xg 1 000 rpm 4 C 5 B7 ES Medium 4 mL B8 B7 37C C0 ES Medium 3 5 1 2 1 200L 3 2 3
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