平成21年度戦略的基盤技術高度化支援事業
Contents
1. noo o noe oo TES dT EE g200 a caa T aa mar EE E Ei noo g200 OOOOD E E E a a E E madang Ej ttr L3 OOD GOOD p mur ES ed an e o E rj Er Eu EHE EE EJ p mmu p mur 63 03 02 63 o00 o go Eri noo g200 3 52 63 53 53 aa noo E oo noo g200 pnmum m 2 OOO oo noo Fi a g2000 3 2 GU ooo oo Er EE lt eae EE a x oooooo oo EJ ai p mmm go000 3 2 E E noo Eg m p p nmur Foon oo ENEE g200 g2000 ET E esl ie Ep ES ES ES EE noo g200 g2000 m SS EE IET oo Ei urn 1 3 Sees madah oo noo noo nooo Tang con oo oo r3 m aoe ES ES ES a E Sie EXE Ed Epp Ed ES EE noo 3 rn p mur Hd Aaa oo en 1 E 3 rn 1 a a 3 C3 5 p c3 go000 ma noo 3 rn g2000 m joo x20100 oo noo 3 rn g2000 i m ii oo noo 3 200 g2000 Grog a nd ma noo noo oo oo nooo Seva aS 1 SEXES 3 2 62 2 oo nooo 3 2500 1 3 6 ooo go000 oo P E 3 5 C c n 1 Gr ld oo nooo lr g20000 ooo ou2. DO Dodo oooO NS 12000000 od 0000000 pod dodo doo 000000 000 12000000 m m UUIUUUUDUUUUUUUUUUUUUUUUUUUUUDUUDUUDU m OU ui 000 OU OU ooogo OUI 11111111111111111111111114111411101 1114110110111111111111 111111 ut 00000000000 aa A oaa A oO 1111 EE BEER DET EEB BED ETHEL DDIAU 0 0 UUUUIUUUDIUUUUUUDUD Ooooooo OHO OOO OOO OOOO O L
3. 0000000000000000 90000 000000000000000000000U00 0000000000040 2004 g m 10000 000 0000 0000 000 800 00000040 00000040 00000040 1 00000040 00000 00000 ng 5 190 10 0 8 00 0 0000000 rn O O 00 00 000000000 EJSAD FH HL D D U DL D BEREAN RENAE AE EE E ET E gt X010000000000000000000000000000000 50000 O 0000000000000000040 OO 31011010 0 00 P PAL0 0 D podg0000000 0000000 0000000000000000 00000000000000000 XDUUUUUUDU X X0000001PX00 000000000000000001PX00000000000 IPPAOOOOOO 000000000000 2 00000 O 00000 O 111111 1111111111 Um ET ED DIET EF UAE EI EET 00000000 2170 16 FAX087 864 6303 0 0 11 11 OU 11 01 00 087 840 0338 761 0301 HEB HGB 0 DO00000 000000000 0 0000000000000000000000 00000 0 0 00000000000000000000000000000010 00000000000000000000000000000000 000 2000000000000000000000 00000
4. A bum n Frakti on M BSA BCEHR KER MANNFEI M 000000000000000000000000 BOO 0000000 0 10000 000000 000000000 RPM 1640 SI GVA Hypoxant hi nel V ko T Thym di nef V ko T Am nopt eri n V amp ko em HU 0 U O U PEG 1000 0 0000000000 1000 AD WANT COMPLETE FREUND D f co Silver Stain ll Kit V ko 2 EQ TM Advance bl ocki ng agent CE Healthcare pol yvi nyl i denef uori de nmuno bl ot PVDF nenbrane Bl O RAD Tween 20 V ko N trobl uetetrazol i um V ko 5 0 4 chl oro 3 ndol yl phosphate di sodi umsalt 1 5 Hydrate V ko G ne V ko Methanol V amp ko Tri 5 hydroxynet hyl aninonet hane 000000 po 0000000 G ycerol V ko 22 0000000 Tritonx100 900000 0 80 0000000000 V ko O 2 nercapto ethanol V ko Sodi umDodecyl Sul fate 9000000 Tri ci ne Appl i Chen Fetal Calf Serum Hyd one 6 L1 O nmunopl ate naxi sorb NUNT 0 100 OO U TH 000 Model 550 Bl O RADI D DU SAG 00000000 TATEO ATTO d ayAdans Bi oshaker BR 21UM TAI TEC 0000 SHAKING M XER IWK G 200 AND 000000000 H OOOO CMTSUNM CHROVATOGRAPHY PAPER 3MM CHR WIATMNN DO 000 DU DU DU DU DU DU DO L3 L3 E3 p EJ L3 L3 J
5. 4000ng M 0000000 0000600 10000 5200000000000 000000000000 2000000 UDIDUDDD 449Mm00000008000000000 2000000000000 UUDEIUIDDUUUUUUDDDUUUUUUDDIUUDDDPFAUDO DDUDIDUDUDUDU 000000000 400000000000 1041000000 200 0 3705 D D D 541000 00000000000 0000 Giess 000000000000000000000000U000U00000U O0 1P X000 P PA1000000000000000000000 IP PALQ 1P X000000000006 1PPA000000000 60000000000 000 6000000000 crudelP XJIP PL0 00000 JI H 000 90 300 0000 14 nh 0 00 lt 1 O PBS pH7 30 7 700 000 0 0 0 8 0000000000000000000000 po0 0000000 0Wen2000000000000 PB 70 300000000000 UDD BADDDUDIUUD 100000 000000000000000000000000 O 00 300 0 0 060 1P PAL00000000000000000000 000000000000000 10000000000 0 PBS T 500000144600000000000000000000000 198484 D 0000 000 SionaQO 0 00000000 10000000 00 50 00 0 Oing h OOOO 00000000 0 10000000 200000 000000000 O 1 11 000 45m0 000000000 15 L3 E3 E E e L3 E3 E EEE E m EE es na E EJ E
6. 00000000 0 P XD00VY50D000000D00 00000 0000000000 1 P XO IPP21900000000000 IP X0 1 0 0 0 0000000000000 000000000000 11111 1111111111 11111 111110 ET ELT 1 1 1 1 DD O BD DD DO DO DD DDD DD DADO DO O O DO ODO DUYU DUDUL DUDU IP PAN O 1 I PAL0000000000000000000000000000000 18 0 0 DODOD00 00000000 oo oo Ei jun ag ma a ooo oo o E 3 EI E ES El Os 000 Sas ae oo AE EE oo oo EY CN IO ee mn ooo lt gt oO oo EE EE E EJ KESE E E E E oo SaaS E E oo man ua 3 3 Ooo oo oo Pa ETE DH oOo a m EXE oOo oo EY ma m Ena ES EE Epp ma oo oy een O y rr fa Sie eto ce THO oo oo m s m E EJ To o a oo oOo r3 r3 A 3 7 L3 7 Ton oo ma L3 L3 r3 r3 r3 n a EE o EE E E ono r3 Sop 1 ESL C3 2 01 oo On cm am os Ou oo L3 EN EEE Ea eias OO 2 L3 r3 oo o E3 FJ E3 rr OA oo oo oo ET E of
7. gt Li mal us 1P PAL0000000000000 OO 4 700000 NODD O P PA100000000000000 I PALO000000000000000000000000 uggagua 000100 0 06000 IPPA49 000000000000 OOOOOOOOOMOGOIP lt OOOOOOOIIP PAGOOOOOOOOOAO 1 IP XI p IPPALQQ 0000000000000 nog 4Ell 34 2048 B2G 49 2 86 F12 PBS 0 049 0 05 0 055 0 048 0 047 0 049 0 053 DD 1P PAL O 05 0 444 0 715 0 559 0 768 0 537 0 466 1P PAl 0 047 0 463 0 756 0 578 0 667 0 526 4 1010 0 048 0 049 0 049 0 052 0 05 0 052 8 nnne 0 046 0 048 0 051 0 046 0 048 0 059 0 046 OOO000000 1111 1 11111111 11 11 1 11 11 1 1 11 11 1 1 1111111111111 HH E HET E EL EE EE 2000000000 D 2 g ng 19 9 29 1 8 01 n d 3 09 n d n d 43 6 37 4 O 990 n d not detected 0000000 EF ED ELE EL E 1 1111 EE EET ETE EEG 1 111 1 P PA10 OO 0 0000 NOOOOOOOOOOO BUUUD
8. E3 E3 i OL 0 9 00 00 00 00 00 00 00 00 00 00 00 enu 00000 DOOOOOOOOOOOO 20 9 0 5004 J po0000000000000 1P XD AD WANT COMPLETE FREUNJ Li f col J 0 01P X0 00O 504000000000000020000 000200000000001PXx0000000000000000000 4 0000000000000000000 DO E4KSA000000000 00000040 00000040 00000040 00000040 00000040 000000 1P X00000000000 1P X 00000 Sianel I D J IP X 0 NNCIMUN PLATE MX SCRP 0000000000000000000000 4 100000 200uU vell D D D E 000000000000000 00 05 Tueen20 0 vel 1 3000000 0 SuL I IIu uu UDD I IIu Uu I ID Ll UD gt 00000000000000T salinre0 Od 3 00000000000 l9GGAM 00000 000 111 37070000 1 0 051 0 500 001M 000000000 0 1M00000 0000000000 gt 000000 2 4000000000045100000000000000 U BI O model 5500 00000000000000 E m o I L3 LJ g BALBE c D D BSA PBS O0 0 10000 0 00 00 00 00 00 00 L3 L3 EJ 23 Western Blotting 0 00000000000000 Tri ci ne 5 PACEI H Schagger 0000000000000 30 5400000000 0000000000 158000080000 00000000 137 5nMTris Hd 4 SES 10 2 ME 0
9. 000040 L3 E O 61 11 21 E OOOO E EE EE DODODODO O O O 00000004 000040 00000000000000000 3 0000 U 000 U o 3 o m jam oo E E Ci A El ES Oo Co oo m oo o o m oo 1 a E aa o ma oo o o oo oo oo oo 3 El El oo oo oo noo o oo oo L3 noo E EE ma EE noo noo oo oo Ei noo El DE oo Bae L3 o o noo noo OOD noo AEA A noo noo noo noo noo noo Ej ctp EX ES Eds noo noo c rn cn OOD noo L3 noo noo noo oO ooo r ooo noo oOo oo UU D U L3 L3 L3 L3 L3 A L3 L3 E Ooo E EE E 231157 E 00004 El E El ES ET Tz E Ei T O Ll 543 EJE 8 EE 0 16 TE Co Em EEN ET E OOO OOo L3 L3 00000000000000000000000 0 0 111111 1 0 O 0 0 D UU DO DO 001P X000000000000000000 00
10. FB 10 OOO000 RPM 16400 000000 50g J ID 699 0000000 8 00000000 HH0000000000000000000000 26000000 37 542010000000000000000400000 0000 111 00 0 1000000000 SOLLI 1 wll 1 1 1111 ELISA 000000000000 000000 wi 8000000000000 000000000 000000000000 50 0010 1111101110 1 000000000000000000000000000 206 00000000001P XxX000000000000 5 DU OU 24 ono 4000000000000 0000000000000 1 10 grig Sou O O O 000000000002000000000 2000000000 0000 0000000 0000000 0 8 2000000 815 000 000000000 podg000000000000000000000000000 00000 200000 000 0000 0000000 00415 0 7 14 21 28 35 42 49 56 63 70 77 84 91 0 DO Western Blotting I 0 0000000000000 IP XQ Tri cine SISPACE
11. Tag E EE oo See ELE oo r3 rm 3 n3 Ooo Eri iE oo E rm E ES 3 5 E r3 n3 S EE EE ES oo a EXE ES ES oo E 3 rl A anu OOD oo 3 2 oo oO 3 7 L3 r3 r3 gang noo oo 2 E 3 ROO EKEK oo cs ee a E nasi a a Ei 3 oo Ee ooo 3 3 EE Ex E E3 se gt l EP ES ooo noo ooo E E EE r3 0 BEE See ooo Ex EE ara a ES Ei Ec og2000 EE OOD ooo 3 3 oO Om oo 3 7 c3 rr rn OOD ogo ooo 2 oO E3 E3 EE L3 7 c3 rr rn mas man ooo Ed 650 EE oc E r3 7 c3 rr rn oX E ooo E E3 rn rnm a EE ELE r3 7 c3 rr rn mas E ooo ET ES E oo C rr rn OOS p rm apa gt x E ET E E E man 3 m ms EJ KE ET amp sar i a E ES ooo Ou o0 gp ono EJ a EE oo oo op a Ea OFo sy 22500970 E ma ma 2592250 Ea El GEL gga NUT ET 5 oo oo mi ooo Exe E gD E ET Ei 3 7 c3 rr rn ogo or Os m coca oa EE EHE 3 a p rm me mE oooonotn OOD EEE 3 3 2 ooo or E ooo ooo eae oe ooo crm c H pm ES mad EEE EEE oo mpg c3 3 g LE ooo ooo 3 22 2 EEE pc gp iE EXE BL E See E EE Ej E E EXE SS ka fa
12. Ena omo oa A noo OOD OOD Yt au rm 3 O DO 00 00 000000000X00 8B0000000000000000 19 36 40000000000000000000 90 C3000 00000000000000000000000 200u g nh m L3 H 1 CI 0000000 20 0 0 0000 0 00000000 00 lnyng OO 000000 20 ppm OO 2 ppm OO 30000 g OO 00 300 g OO 8 5 gt gt o o o Ell E 3 E 21 nm E El edited 11 E pap TT nm 0 DO00000 00000000000 podgoaDbo0 0000 oo 00000000000 000000000000000000 00000000000000000000 00000000000000000000000000 000040 0000000000000001PX000000000 EUSAODOODODODOOOOOOOO 00000 1P X0000000001PX000000000000000000 000000000000Bcdl00000000000000000000000000 000 000040 00000 BABc00006000006m000000000000000000000000000 DD P3 X63 Ag8 UL P3U1 0000001P Xx00 900000000000000000000 Dul becco s Phosphate Bufferd Sal i ne Si gna Anti MusePol yval ent nmunogl obul ns IgG M AI kal i nePhoshat aseConj ugat e Anti body devel oped in Goat SI GW
13. rn LJ nud PB8e2L82250500000 e EME L3 rp L3 E pm L3 L3 C3 p 2 3 E3 g L3 L3 EJ pr nooo 5 3 3 L3 1 P3 r3 rn rm AE o Llr ET 3 4 L3 L3 84 L3 r3 cC E L3 L3 P3 rn rm A o Op 2 L3 L3 L3 r3 E3 E3 EJ Oooo L3 L3 p L3 L3 C3 E3 E3 EJ mem EY EET ESI ES L3 o o E3 EJ E3 E3 E3 C3 E3 r3 L3 5 HH E Eh EE TE EE i EE OOOO Em 1 2252350020 2250535020 2 MA ie a en EEDD ET EE E 3 5 ee EL E 16 e Ed dl Oooo d aana EJ dH pr c C HI Oooo oon Oooo arnon H L3 EJ EJ sooo EJ EJ E3 E EJ HJ EJ EJ sooo 03 3 EJ c C3 C3 EJ E adad C nad E Co 0 DO 00 OU 00 O EE 1 LJ 3
14. 2 4 LJ 4 D D pod0000000000000000000000000000 00000000000000000000 2805000000000 o EE E Et E El 000000 00000040 000000000040 00000000 0000 0000 000 uu L 000 Oooo ra E 0 4 E El Ou Da aha a Ou Da aha Ou Da aha Ou Da ada CI L3 r3 Oo goto a o g 1 L3 Da aha a Au Ta ada 5 3 Er a om 28450 oO L3 CIE E EN ea El ODO E es m Do 8000750 c3 e Tm nm im ua 2002090525000 ES aE ma un imn mn Aro a ma oO ES EE 50519000500 oo n H EE O oe oO Ez A sg y o000000 0000000000040 0000 00000 00000000 0000 000 UU 00000000400 0000 0000000 000 00000000000040 0 00000000 22002011 02000040 00040 0000 000000 110 100 0 00000004 NN ri c ANA 6 6 6 9 0 00000004 c SKS 000040
15. 00000000 OOD 000000 0000000000000000000040 MMDOD lt 0000000040 000000 00000000000000000040 A 0000 woo 4000 8 0000 2000 27000 000000000000000 0000000000000000000000U0U A 000000O000 5 000000000000 C D 000 000 e DOdo00d00000000 000000 pIsgo00 0800000 0000000 1300000 73000000 390000 25080000000 121 150 0 0 00000000000000 sm0000000000000000000 0 34000000 1111111111 11 1 EU ORBAN HEA AH BNE 00000000000000000 o0000000 00000 0000 00000004040 00000000 90x 15mh I WK 000040 UDDUUDDDDUUDDDUDUUD Model 680 Bl O RAD 000000000000000000000 version 5 2 1 00000 SLI 220 EYELA 0000000000 MR 162 SANG 0000000000 MM1 IWANG 8 00000000 Ur 305 5 SHARPO 00000000 0 TX 160 TCM e0000000000000 000000000 900000000000000000000000000000000 00000000 1400006m00000000000 30sex1000000000000 000400156006400000000000000000000000000000000 0000000 104100000000000000000000027000000 200 000000000000000000 200000000 co OOD a E noo oo agan 3 3 janma EEE 9r Bla EA maa janma noo aa SEE o520 Hoo jaman EE ma ELE 3 ooo ma gt Ed E ies OOOpoooono noo Oo ala
16. 0095 Brono phenol blue 1496G ycerol 0 O O 95 CO 5000000000 OO 222000080080 0800 0000000000 TicneSDPAF 00000 000000000000000000000 Silver Stainll Kit V kor 00000000 00000000000000000000000000 25nMTris 192nM G yci ne 2096ethanol 0000000000000000000000000000000 uu polyvinyli denef uori de nmuno bl ot PVDF nenbrane Bl O RAD 00000000 TATES 0 0000000000000 0000000000000000000000000000000 30 1 50 0 0 0000 2 E0_TMAdvance bl ocki ng agent CE Heal thcare O TBS Tri s Buffered Sal ne 150nMNad 2OnMTris pH7 5 B B UH 0000 O 05 Tween20 V ko 0 2 Tritonx 100 1110100110 TES TTI I Ui I Ii I 1 i Ui Uu I i I i I iu BSAPBS 28 0 2 0 0 O Anti MusePol yval ent nmonogl obul ns 1 gG A M Al kal i Phoshat ase ugat e Anti body devel oped in Gat SIGN 0 0000000000 1000000 000 3709 10 0 0 2 1 1 0 0 0 0 033 Ntrobl uetetrazol i um V ko 0 021 5 Brono 4 chi oro 3 i ndol yl phosphate di sodi umsalt 1 5 Hydrate Veko 000000 000000000000000 0 5 m000 370 200000000 6 000000 400000000000000000000000000000000 8
17. 00000 322m 000000000000 0 50h mh 1380000P X0 D B nO BO DO DD DO DDD DDD U O IP X Li nulus V i ght ng ng ng P X 290 60 NT P X 120 0 6 42 NI not tested P XI P PAL P X P 5496 67 6 6 196 lt 5 1 396 lt 5 gl ucan 1 7ng ng NT Li nul us 2 91ngy ng 1ngj ny nain 5kDa nain 5kDa minor 100 12kDa 25kDa 40kDa minor 30kDa 6Okda NII not tested 12 lt 0000000000000000000 000000000000000 5C6PAC OO 11100 0 0000000000 4n eap 1000 25kDeQ 0 0 000000MOOODOODOODOOOODODIPPAOD000000000000000000 1 0 0000000000000000000 0 0000000000000000000 ri U 1 SO PAEQ OO 1P PX0000000 oe MW43 000 RNase MW14 000 Vo LPS Y Y Y LPSe IP PA1 IP PX 0 5 10 15 20 25 30 Retention time min OOO IP PALD 1P X D 13 0000000000040 0 0000000 00000040 00000040 DU Ex E E 1 151 g DO DO DO EE misi 3 8 63 62 3 2 3 2 11 m a Pyr Pao uu See a Ol ag SER 5 e Po
18. a aoga H Y ma Co a Hal a d 9 g ma ma oo ETE o EI Oa ou se ends i E CES Ed Ed js Oo oO oO Epl lt E ej E G EJ EJ e 5 3 2 gt s a jm Lm 8 or o q EA EJ L3 p HEX L3 IP XI NOO 0000000 0000040 0000 000 IP XO 1 IU Y30000000 Lina us 000 0000000000000000 30 IP PALDO 000 000 IPXO 0000 SD PAED PLSOODOODODOOOPPAODODODODO0MOOOOODODO 000000000000000000000000000U00000U 14 6 00000 lt LH d d uu lt ipidADDDUDUDUD OOOO 00000000 ono RAWC4 7 ATOCND TIB 71 ano 1P PAV Pantoea agg onerans OO IPPAUJUDUDDUDUDUDUNU 000000000000000 00000000000000000000000000 i P x0 000000000 000000000000000000000 00000000 0 00 N000000000000000000000000 0000000000000000000000000600 1 FGC00mO00000 50u grim OOOOO 604 RPM164000 00000000 8x1009 m0000 000000 1001 8x 10 0 1004 1 60000000000000060000000 0 600000000000P XO
19. n D 3LO0000 D D UD D b OO 7000rpni 00000 ATCNo TIB 71 0000000 000000 0000040 0 0 0000 HL 77081 NEWENGLAND BioLabs 0 00 00 000000000000000 Gt N 30642 44000000000000000000 0 0 00000000000000000000000000 00000 00000000000000000000000000000 1000000 O IP PAI Fantoea agg onerans I I Uu uu u au UU L 1 0 00 0 0 0 0 0 Prestai ned Protein Marker Ln d gu 00000 e Epl a lt a be fin ma e 10 Sooopugogougoo 52035 05 D Ur rey E NO oooO D 28 02652 22 580990 5200500500 um n HR ta E E EU ag ra 2090077 0200600 o gbaoaocud 3 35 6 qr aaa oa 2200500000500 a Oopgfoopgoooooooo oog200 5320000000 00000500 2 6203 58 s C3 Lg DL pcr Ou C2 C3 C3 8 C3 s Ea E e leia 1001 e ES Es 222 9 2 2 2 2 amp x FF oo BCA Protein assay kit PERCH 0000 BALI I I lt o0000000 OU
20. 00 000000000000000 mea T E ETELE ET ETE EQ EIE ro EE EI 14 El EST E E EE 0 EE EEE L3 L3 E3 E 1 11101 54900000 OO crude Q n 0 0 1P XD D OAO 00 6090000 0 DO 1 0 000000 poeo00 m0 1 0 1 HD 1P XO 11 5 t p 0 0 00 0 D IPXIDDDUDDUDDUD 40 O DOM 10 690 000000 1P X0 11000 0 000 00 1P Xx00000000 49000000 0 DOXD000 0000000 Li ral us OOOO IPP100000000 12000 00000000 4000000000000 OOO 6 285120430 D D 1P PALO O D 10000 290ng Li nul us 000 0 D L3 300000000000 001 X000070000000000000000000000000 20000000 Dg pg gon gg DD O E O DO DO DO DO DO E BD DO O DD DDD OD oooO 54 6 0 0 O 0006184000 00000 28
21. 00000000000000000000000000000000 He oo 000000 000 260nm DD DA 32007 000000000000 10 00 504 g m o0 Q man 000000 000000 Limulus assay 00000 555 1 1 11 11 100 000000000000000 Limml us D I U H U 1 P PA1 uU mulu 3 3 ES ES EE En mn oe EEE nooo Ed Ert ET Ej ES 00000000000000 6000 B 000000000000000000 GUDD 00000000000040 69 SDS PAGE 000000 O 1 00 0000040 Tri ci ne SD amp PAGE po00000000 00000 000000000000 5 000 0 0000040 DD 000000 Su glare 0 HPLC O 000 0000 20 3 00 I LE 208 OOO 0000 SPD 20AMT 000 00000000 CC 2060 000 TSK gel G3000SW o 7 5x 300 0000000 000 LC Sol ution OOO 00010n Mm0000024100000000000 100nM 000000000 pH 5 0 1 mn 210nm 25 C Ed EE Lh El Oo LI E E ue y d ES D 0 Lr CO mn 00000000 XN00000000000000000000000 0000000000000mM00 10 G070MO00000 00000 6 RPM 16400 0 0 0000000 8x 1050 m D H 1004 8x 10 0 1009 60000000000000M6M00000 60000000000 2000 3 d5800000000000 0000000 50 00000000006M0000Giess 0000000 0000000040 0000000040 Ed E ELI LL DO 11 5000000000 000
22. 005000 gt ooooo S EET 3 03 aa a mi CR SaaS EL E E E Eg e gt ooo e O 3 70000000 o A IDD 4 7x 105 0 5 0 0 8 4 6x 10 1 4 1 1 000 3 9x 10 2 5 2 2 PLEA D 9 2x 105 0 2 0 0 E 5 6x 10 2 8 2 1 F 2 9x 105 8 21 8 1 A DDD 5 1x 10 5 6 5 4 8 1 7x 105 5 9 5 4 00 000 9 7x 105 4 4 4 27 2 D ODO 1 2x 106 5 5 5 4 E ooo 1 8x 10 8 13 8 4 DD 1 4x 105 10 12 10 2 8 0000 2 9x 10 5 5 5 2 a HM 0000 3 3x 10 6 6 6 44 1 Dau 0000000 19x10 12 14 12 2 E 3 3x 10 11 12 11 3 A DDDD 1 8x 10 0 6 0 0 A 80000 2 5x 107 1 19 1 1 A DD 3 4x 10 1 11 1 1 ooo 8 000000 3 9x 105 2 19 2 5 2 000 9 2x 105 2 20 2 2 0 10900 15 18 1 1 1 0000 1330000000 27 000000 400000000 1000000000 10000000 400 UD 1200000008000 0000000000000 0000000000 000000 280000000000000 000 UDD 000 DD NO 00 302 000000 OOOO H TAH SCR 20B Broad Range Prennxed For mat Cat 000 320
23. 3 p3 E3 p3 E3 E3 E3 E3 C E L3 p3 EJ EJ p3 E3 EJ E3 E3 CE E L3 OOO oO E3 m E3 E3 E3 L3 L3 L3 C3 L3 r3 E3 C3 E3 EJ E3 E3 E3 L3 L3 E3 C3 C3 r3 E3 C3 E3 E3 E3 E3 L3 r3 L3 C3 L3 4 r3 C3 C3 E3 E3 E3 E3 E3 E3 E3 o0 ae 20 ma aag JEN E ENE gt HA gt CE E po rm 3 EE OO rm LE Eek GERD Gg EE 4 rE ELE E E E ET E E EJ E a L3 OEGE E ET MEDU Lla OOO 2450000 ooon A MEA A leer aie 3 3 872 OOOO 550000 ooo a 3 2 52 OOOO 55000050 0000 a um Oooo 3 3 3 Arp Oooo 3 3 3 3 34 ud Oooo an pj a cer EJ maan p E EJ EH EJ Oooo Llr E E OOOO Sa Ss EE aaa L3 L3 p BE Lr HL Hl OOOO o L3 p HL Lr HL 8412040550408 ES m Ed EXE E E opnan OOOO
24. Eel EE L3 L3 E3 EJ L3 L3 E3 EJ E a ag P Xx0 9nm9 0000 9804100000000000 mn 3720000000190 00 EE m nm O EU 00000 OU 000000000000 0000000 RP 10Xx 00000000 TSK gel Sugar AXG 4 6nmh 15 0000000 O O 70 C 0 5mol L 00000000000 8 7 O 0 0 4mL min ww 00 0 000000 000001 Ex 320nm E m 430nm 000 3 w v 4 OO 0 5mL min 50 C OU TSK gel Sugar SCX 6nmh DIx15cnl 00000000 60 C 0 0 16mol 1 1 H PH 7 6n O 0 0 3mL min 0000000000 lww 00 000000 000001 00 3 w v d 00 0 5mL min 0 50 C 00000 Ex 320nm Em 430nm 16 o 1101 00000001P X000000000 000 000 000800000000000000000 9 0000000 0 IP XL 0 RAW264 7 OO B0000 LipdAD D HDD U 1P X1000000000000000000000000000000000 RAW64 70 ingmp Limus 00000000 O RAW64 7 IP PA 0000 T P Co gt gt oa 5 x x aa A A MO O o ri ri r aun 100 1000 10000 17 OOO ng m 10 0 1
25. I I 1000000000 0 V sternBlottingO 0 00 0 D 00 0000000000000 400 Wstern Botting 0 0000000000000 OU 25 l Brade L O Holst P Kosna Y X Zhang H Paulsen R Krausse and H Brade 1990 Characteri zati on of nurine nonocl onal and nurine rabbi t and hunan pol ycl onal anti bodi es against chl anydi al popol ysacchari de nfect nmun 58 265 2 Tani guchi Y T N shi zawa T Honda N Yoshioka H Inagaw C Kohchi and G Sona 2007 Devel opnent and potential use of a nonocl onal anti body to the popol ysacchari de of Pantoea aggl onerans P PAL Anticancer Res 27 3701 3 Schagger H 2006 Tri ci ne SD5 PACE Abt Protoc 1 16 4 Honda T H Inagava M Fukushi na A Mori yana and G Sona 2002 Devel opnent and characterization of a nonocl onal anti body wth cross reactivity towards uracil and thymne and its potential use in screening patients treated wth 5 fluorouracil for possible risks Gin Cy mActa 322 59 0 0 000000000000000 ELISAD UDD P XOO0000000 E sap II BD 1ng g 2000000000000000000000 L 00000 I SA Enzyne linked nmuno sol vent assay O00 26 1000 0 00
26. ng 3 maa Eb ES Ee a noo E el ET ooo Ta oo ej peus oy noo Oo nHHrm r3 oo EX c ti EE ea 3 L3 C3 p3 C21 Geo noo E B52o 6 3 53 53 ao ooo a ogo Ou oo Rt a a SSF Sas Sen mH madad ER EDS Eau r ORO aS AE Ei E Eis EJ Ede an EE o7 gaya r3 r3 p E Ro 3 5 r3 n rn oo nooo 3 ooo mnoooo rd EEE E 75530022 9 22 22 22 3 ej pg m nooo Sas ooof puma mmm aso nooo E es mang 580220 p2ooolc poo nooo ER 53 53 53 oO 94202 22900575420 nooo ET Ooo L3 r3 ooo o 205058700 AE a En ata 5 o gt _ nooo Om 55270082 EEE EU EE IFE nooo ED ENE ENE madang np 22295007050 9 rrnrmrnr EE madang Ee iia OOOOND ma ji ma madang gadah 2000 madan 205500000 madang FooooO0ooo ooooo Sao ah na E 2 2 200300000 EJ madang 3 1 1 ooooo gt 2 E3 Ed Ed EJ EJ m 13 L3 0 O AN 2 poa ooo eos za tS Eje oie oooooCooo nono goof goon ooonooCoaoo ui 3 ritirtrirrt amara OOD a Aroca aa 5
27. r go000 ma E ES E EI C3 c n 1 1 C2 C1 c3 5 c c n iii EE c5 C c n 1 aa DOD c c n imi oo Ai ra Pj EI mjang s a Esi ooo OOD Ed E E EI C3 7 C1 c3 n3 1 3 mangana oo EE EIE El t EM g20000 8 285005205 oo nooo rn g20000 59 1 11 07 1121 ES a 250052053 oo nooo 3 1 1 EKE EL Epi 8500505 oo od a 1 EE e ses 2 228 00000 oo 9 an EE EC SDE LJ vas aon Ed rs ET Myang oo nooo a Sava a aoe 3 oo cr uu oo E EXE Ed HEETE ES EE EE E E EA EE ET Ea E3 b E3 DS Boo oo E E Ss CES EE EZ EE EJ Ed E E ESL ET awan Ei ile EXE oo 3 3 E00 mne G9 8 Ty 6 oo 27
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