ZEN2012 Quick Start Manual for LSM710(日本語)
Contents
1. i Carl Zeiss LSM710 780 ZEN 1 1 1 1 1 1 1 1 2 1 1 3 Ar 1 1 4 NLO 1 1 1 Main Switch ON ON2. 3D 3D 1 Z Stack 2 3 4 Z AF Le FE Lm ini 1 Z Stack Find Focus Set Exposure Live Continuous snap Z Stack af Stack 21 Slices Time Series Bleaching Regions Start Experiment Fig 33 Application Show Al fit 2 Z Stack First Last
3. Locate Locate Acquisition LSM 5 ON OFF
4. 85 15 15 2014 2 ZEN2012 SP1 36
5. Fig 49 Player a Fm Carl Zeiss LSM710 780 ZEN 9 4 Graphics Sa Auto Color Fig 50 Graphics Angle More Coordinate Cutregion a 6
6. 2 LSM CZD HE Carl Zeiss LSM710 780 ZEN 1 LSM lIsm CZI czi Save Image Fig 27 New Image DQ File Save Save as Fig 28 peichern unter Speichem Desktop i JEigene Dateien demo data WW Arbeitsplatz 2 Jenakultur be Netzwerkumgebung JofBioTech_CallforPapers CiscoSecure Authentication Agent LSM 4 2 Icons Div Movies Movies 2 Axiovision 4 6 Icons Music lt Dateiname Image 2 TE TE Datetyp TSM 5 lem meee ha Abbrechen Fig 28 LSM lIlsm CZI czi
7. Closed Fig 8 Axio Observer Z1 Configuration ie Et UG DAPI FITC pdaminE OFF DIC Fig 9 Carl Zeiss LSM710 780 ZEN 4 Acquisition LSM 2 4 1 Experiment Manager 4 2 Smart Set up 4 1 Experiment Manager Experiment Manger Fig 11 Recent Experiment Manager
8. 0 Carl Zeiss LSM710 780 ZEN Microscope Objectives NE objec re opto Light Hone tives act var path Load position Control Ea TL Illumination a 10x Automatg PL i Display RL Illurnination OFF On Reflector Re Flector Ypto Light var path Objec Home tives Tlmmlll Load position LL ee Pos 6 Analy DIC Trans light _ Contrast Manager Display 63xJ0 75Ph2 0 DIC TL 2 04 TL Illumination On Off RL Illuminati
9. Imagel7 ism ec 50 ME x Fig 26 Not Saved Fig 25 ZEN Fig 26 ZEN 2 1 LSM CZI Carl Zeiss ZEN lite Windows OS http www zeiss co jp microscopy 2 Export
10. 1 0 THES Ff all amp 00 0 0 Reset All Mace BM aon Fig 24 Scan Area Pea Reset All a Eee er ae od BE 12 Live Live EH iO 3 ul ist A nk ne 0 Q ey LET s 47 LSM710 780 ZEN Carl Zeiss 6 6 1 2D
11. Ch1 Ch2 1nm Color Detector PMT Dyes iE TRARY ae ae Carl Zeiss LSM710 780 ZEN 2 Simultaneous Sequential 2 TT Simultaneous LAL
12. LED of Fig 1 1 1 4 NLO JELLY Ft Chameleon Standby ON Chameleon ON 1 2 a ZEN A Start System Login Tih 2013 ZEN Fig 2 Start System Image Processing Image Processing
13. ovl Se Carl Zeiss LSM710 780 ZEN 9 5 Series 3D 3D a 3D 3D Fig 51 3D Series X Y 1 Render series X Y RE 2 Total frames 3 360 Difference angle Panorama CHIPTIPE Series NN Fig 52 Apply Player 9 3
14. Boot status Fig 2 Carl Zeiss LSM710 780 ZEN ZEN 3 Fig 3 es Locate Acquisition Processing Maintain Fig 3 3 Fig 3 Fig 3 ZEN ZEN 30
15. Recent Alexa Fluor 568 Alexa Fluor 488 DAPI Alexa Fluor 594 Alexa Alexa Fluor 350 Alexa Fluor 405 Alexa Fluor 430 La by Alexa Fluor 488 Alexa Fluor 532 Alexa Fluor 546 Alexa Fluor 555 Alexa Fluor 568 Alexa Fluor 594 Alexa Fluor 610 Alexa Fluor 633 Alexa Fluor 647 Alexa Fluor 660 Alexa Fluor 680 Alexa Fluor 700 Alexa Fluor 750 Fig 13 Smart Setup Fastest Best Signal Smartest 3 Apply Fastest Simultaneous Best Signal Sequential Fastest Smartest Sequential
16. a Laser Laie Laser Lines neni Argon 458 488 514 Donde 405 0 Abi EKITTMHITI Foe 50 ri Wavelengin 63 nm Status He Fig 7 Blue Diode 405 405nm COMPONENTS ON Argon 458 488 514nm Page 2 DPSS 561nm HeNe 543nm 594nm 633nm ON NLO MaiTai Chameleon ON NLO Laser Properties 10 15 Carl Zeiss LSM710 780 ZEN 3
17. Sequential s l Carl Zeiss LSM710 780 ZEN 5 a Channels Track 1 Airy Unit Fig 16
18. 488nm 1 5 A Carl Zeiss LSM710 780 ZEN Range Indicator Master Gain Digital Offset Range Indicator Dimensions Ch Lame Range Indicator Dimensions ce a Chi TPFMT ia ea Single Channel Range Indicator Fig 21 Dimensions Wf Merged Bs go veoyyot SLHRWAS UALAN Range Indicator 0 Master Gain Digital Offset
19. Acquisition DAPI FITC Smart Setup Show all Tools AF mf Go Find FocuX Set Exposure Live Continuous Z Stack Time Series sik Bleaching Regions Fig 17 Set Exposure Fig 18 Live fey cal BIT since Live Live Continuous Set Exposure Find Focus Master Gain
20. Master Gain 1 Averaging Live Continuous Leese 3 ae Carl Zeiss LSM710 780 ZEN Merge Dimensions Merge ON OFF Fig 19 Split Channels 4 Channels Tracks Channels y Track1 DAPI Cy3 Ch2 Select All Unselect
21. 2 2 3 5 4 4 3 3 HBO Field Stop Aperture Stop FL PSC ON DU PWN a BG c Carl Zeiss LSM710 780 ZEN 11 OFF OFF 11 1 DPSS 561nm HeNe 543nm 594nm 633nm NLO Setup Manager Laser Off Chame
22. Data Raw data Contents of image window Hist Full resolution image window Z single plane 1 series Movie 1 Compress x J0 Carl Zeiss LSM710 780 ZEN 6 2 File Open New File
23. 1 8 2 Split Dimension Merge ON OFF Fig 38 Split 8 3 Gallery 3D 4D Gallery Z Stack Time Series Fig 39 Gallery 24 Carl Zeiss LSM710 780 ZEN 8 4 Ortho Z i kh Ortho Full resolution Export Fig 40 Ortho 8 5 Cut Z gt wal Cut
24. 1 Mo ae 0 67 Lim nterval Slice Set First Z Set First 10 00 Fig 34 Z stack Set Last Z Stop OD Carl Zeiss LSM710 780 ZEN 3 Z Smallest Optimal Z w Show Al ie O First Last Smallest Optical Slice Fig 35 Optimal 4 Start Experiment Z
25. Boon lela Nl ttl i Z PE A nN aor Channel Range indicator Quick Color Setup Reuse Crop PaA La RRO HATES ED Fig 47 Dimensions 2ch Zoom Merge Single oe sauce cle Channel s ON OFF Crop PHYA Reuse 9 2 Display Desplay Auto Min Max Bestfit nan oo 9 3 Player Z
26. Fastest Carl Zeiss LSM710 780 ZEN Show all tools Light Path w Show all Tools New Fig 14 Show all tools Light Path Track 1 Track 4 Experiment Manager Ree gt CC ZEN uim MBS 488 561 1 7 Visible light MBS 405 None ne invisible light Fig 15 Light Path MBS Vis Invis
27. 29 Carl Zeiss LSM710 780 ZEN 10 Axio Observer Z1 Axio Observer Z71 6 6 TFT
28. ZEN Show all tools Show all tools Setup Manager Light Path Fig 4 Fig 4 Show all tools 4 Carl Zeiss LSM710 780 ZEN Show all Showal 7 Show all Fig 5 Show all
29. Search Ex 488 GFP 1 Ex 488 561 633 2 Acquisition Processing nment Wianagzer DAPI FITC gt Bimart Setup AF oO Ey ml Find Focus Set Exposure Live Continuous Stack mars Time Series Bleaching Fig 10 Experiment Manager Fig 11 Experiment Manager xu Carl Zeiss LSM710 780 ZEN 4 2 Smart Setup Smart Setup Namie DAPI FITC Configure your experiment Bee area Fig 12 Acquisition Search Dyes Recent
30. SYSTEM PC ON PC LSM User COMPONENTS ON SYSTEM PC PC COMPONENTS PC SYSTEM PC ON 1 1 2 ON ON Carl Zeiss LSM710 780 ZEN 1 1 3 Ar Ar Power Enable ON 1 5 idle run LED Power 9 10 LED
31. Cut Fig 41 Cut 8 6 3D Z 3 aD Z Stack 3 3D 9 5 Series Fig 42 3D 8 7 Histo Histogram Fig 43 Histogram 05 Carl Zeiss LSM710 780 ZEN 8 8 Co localization 2 Co localization He Fig 44 Co
32. Track 4 Channels cea Tracks Channels Acquisition qCeESsine it ss y Track1 DAPI PXpeniment Manage Cy3 DAPI FITC ni a Ch2 i Smart Setup Show all Tools Select All Unselect All JAF ee feet os rh DAPI th wo Find Focus Set Exposure Live Continuous Snap f Stack Time Seres Vv Vv Bleaching 405 458 488 514 561 594 633 Tile Scan Positions 1 9 Regions tart Expe ent Setup Manager Laser Light Path no Acquisitton Parameter lt gt Stape Incubator Integration Photon Counting A i Multidimensional Acquistton Information On Expenment I Auto Save Fig 16 Channels 12 Carl Zeiss LSM710 780 ZEN RO BBR Set Exposure o Master Gain Digital Offset Set Exposure 100
33. Fig 22 Range Indicator 1Airy Fig 16 Gain Master 800 1000 Fig 20 Channel Fig 20 3 45 Carl Zeiss LSM710 780 ZEN Acquisition Mode a Acquistion Mode Acquisition EC Plan Neofluar 10x 0 3 DAPI FITC Smart Setup Show all Tools Frame AF QO Goa Ga Find Focus Set Exposure Live Continuous Snap 512 Z Stack eke Time Series x Bleaching Regions Setup Manage 9 Laser Light Path Fig 23 Acquisition Mode Acquisition Parameter Acquisition Mode Frame Size 512x512
34. ON OFF f Light Path t a Online Acquisition Fig 6 ZEN Workspace Configuration 2 Carl Zeiss LSM710 780 ZEN 1 2 3 4 teaser emer 2 Setup Manager Laser ON ON OFF
35. Averaging AF o fest on oy Find Focus Set Exposure Live Continuous Snap wf Z Stack 31 Slices Time Series Bleaching Regions gt Start Experiment Fig 36 Application Z 8 2 Carl Zeiss LSM710 780 ZEN 8 2D 3D 4D X 8 1 2D 8 1 2D Fig 37 2D 2 Z C 2D 3D Time Series AMode Dimension Player
36. Optimal Optimal Speed Speed Pixel Dwell Scan Time 10 8 9 7 Bit Depth Bit Depth 16bit 65 536 12bit 4 096 8bit 256 16 Carl Zeiss LSM710 780 ZEN Averaging
37. LSM CZ Save CD LSM D 2 io Carl Zeiss LSM710 780 ZEN 2 Export TIFF JPEG File Export Select file name and save Save Fig 29 Export Format
38. 23 24 25 26 27 a De Carl Zeiss LSM710 780 ZEN 10 1 1 Locate 2 TFT HAL ON OFF ON 3 H 4 LD N Ww BP WN eRe IAEA CME ST SXF Smart Setup Experiment Manager
39. Averaging Fig 23 Line 4 Mode Line Frame Method Mean Sum Number Scan Area Scan Area Show all Fig 24 Scan Area
40. Light Path T PMT 10 Fig 15 Gain 3 Carl Zeiss LSM710 780 ZEN 10 2 200um DIC DIC 1 2 0 PC 2 DICI DI
41. Browser New File Browser File New File Browser File View Acquisition Maintain Macro Took Window Help Fig 30 File Menu Q File Browser HDD Fig 31 New File Browser Reuse New File Browser Reuse gt ac Pd WI Copy Fig 32 Re use l Carl Zeiss LSM710 780 ZEN 7 3D Z
42. CD Nc A a 36 1 2 3 n 85 15
43. All DAPI th w vV 405 458 488 514 561 594 633 Laser Lasers Gain Master Digital Offset Digital Gain Fig 20 Master Gain Digital Offset Digital Offset 0 Lasers
44. C DIC I 40 DIC I 40 1 LSM SM Carl Zeiss LSM710 780 ZEN 10 3 1 Ph1 lt 3 1 2 10 4 ON 1 3
45. Microscopy from Carl Zeiss LSM 710 LSM Systems LSM Software ZEN 2012 We make it visible Carl Zeiss LSM710 780 ZEN ZEN ZEN Ei APOE A ee en eee ee ee eee eee ee een eee ent ee ee eee ee one eee 1 1 2 2 6 3 7 4 8 5 12 6 18 2 3D eign eee ae ed eee 22 8 DC oo a a 24 9 27 10 tree 30
46. leon NLO Chameleon ON Standby Argon 458 488 514nm idle 0 5 Ar OFF x Ar 11 6 Main Switch OFF 11 6 OFF 11 2 ZEN File Exit Windows Shut Down 11 3 11 4 11 5 PC OFF SYSTEM PC COMPONENTS OFF Main Switch OFF
47. localization 8 9 Profile Profile Spline Fig 45 Profile 8 10 Information 1 Fig 46 Information Carl Zeiss LSM710 780 ZEN 9 Jf Sh Dw all Dimensions Play Ne 3D Show all 9 1 Dimensions Dimensions
48. on On Off al Carl Zeiss LSM710 780 ZEN 1 2 3 L 4 5 6 7 8 1 2 9 10 25mm 11 12 13 LM set 14 XY 15 16 L 17 18 On Off 2 3200 19 RATS On Off 20 TFT 21 22
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