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1. LeuM1 FITC CD15 FITC FITC 4 1 CD15 Leu9 FITC CD7 FITC FITC y gt ey BIER 2 po yf ots dele HEL gt ERNER Y ne sure 42 LeuM3 FITC 43 LeuM3 FITC CD14 FITC CD142. Leu9 FITC CD7 FITC T MM LiuM3 LeuM3 FITC CD14 FTTC LcuM3 8 Er LeuM3 PE CD14 PE CALLA FITC 1 EDTA CD10 Anti CALLA FITC Common Acute Lymphoblastic Leukemia DY vier Ya CALLA A en a PBS x EI FITC CD45 FITC 2 1 1
3. 3 FL1 FITC 488nm 530 15nm FL2 PE 488nm 585 21nm 5 3 FL1 FITCH 488nm 30 15nm 4 3 1
4. LiuM3 PE CD14 LeuM3 PE CD14 PE CALLA FITC FITC CD10 Anti CALLA FITC CD10 e Na yas FITC CDAS HIC FITC iaa ds PERT RAG nn ELISA on CD45 2 Fa 3 LeuM1 FITC CD15 FITC
5. 3 5 50 6 CD15
6. BD Multi Check control BD 340911 340912 3409131 FITC PE Mouse lgG1 BDF 349041 349043 488nm FACS 15 16 1 EDTA 20 25C 2 HUSA IO EP abe ae Ficoll Hypaque Pharmacia BD Vacutainer CPT BD 2x10 zL Ethidinm Bromide Acridine Orange EB AO Viability 90 EB AO lt gt 1 1 1
7. B 05 20200EZY00061000 LeuM1 FITC 20 11 1 20200EZY00059000 Leu9 FITC 16300EZY00702000 LeuM3 20200EZY00060000 CALLA FITC 16300EZY00893000 FITC LeuM1 FITC THB BO Leu9 FITC Il LeuM3 LeuM3 FITC Il LeuM3 LeuM3 PE CALLA CALLA FITC FITC 1
8. 3 2 8C LenM1 FITC CD15 FITC 18 Leu9 FITC CD7 FITC 24 LiuM3 LeuM3 FITC CD14 FITC 247 H LiuM3 LeuM3 PE CD14 PE 24 CALLA FITC CD10 Anti CALLA FITC 24 FTTC CD45 FITC 24 LenM1 FITC CD15 FITC 100 Leu9 FITC CD7 FITC 100 LeuM3 LeuM3 FITC CD14 FITC 100 LeuM3 LeuM3 PE CD14 PE 100 CALLA FITC CD10 Anti CALLA FITC 100 FITC CD45 FITC 100 1 Skubitz K Balke J Ball E et al Report on the CD15 cluster workshop In Knapp W D
9. 19 PBS 0 5mL 3 Vortex 1 PBS PBS 0 5mL 2 6 2 7 er 132 416 114 CAI 1 CaliBRITE CaliBRITE 3 FSC vs SSC SER
10. Free Dial 0120 8555 90 FAX 024 593 5761 S 960 2132 1 Free Dial 0120 8555 90 PI 64 004 6
11. Ca Mg free BD CellWASH 11349524 2 2 2 1 2 wt vol calf serum 3 BD FACS Lysing Solution 349202 20 25C 20 25C 10 488nm 20 25C 20 2 C 1 FITC 530nm 4 1 PBS
12. 2 1 2 20 25C 20L 1 3 10047L 3 Vortex 1 4 20 25C 15 30 1 5 2mL 3 Vortex 20 25C 1 6 20 25C 10 12 12 300 xg 5 50zL ALETO PBS 2mL MMA 34 Vortex LU 300 xg 5 50gL 19
13. CD7 NK pre B BRE ULES lt KU LE TAS RABO Y SER OR LAU L EAE A W 8 CD14 B T NK 9 CD10 B 10 CD10 W8E7 Common Acute Lymphoblastic Leukemia CALLA 11 CD45 N R LeuM1 FTTC CD15 FITC 50 0 93 2 36 0 93 2 34 Leu9 FITC CD7 FITC 60 0 95 4 99 0 96 25 86 0 an LeuM3 FITC
14. 2 1 EDTA 2 3 6 4 5 3 3 5 x 10 9 4x 10 guL 0 1 PBS 4 Ficoll FHlypaque
15. PBS 0 5mL 3 Vortex 1 PBS PBS 0 5mL 24 2 2 1 2 20 25C 20L 50 L 2 80 30 45 1 7 1 8 1 9 1 10 2 2 2 3 2 4 2 5 PBS 2mL 3 Vortex 2 8 300 xg 5 50L
16. 20 25C 3 5 1 121C 20 1 000ppm 1 2 0 1
17. 60 098 045 0 98 6 57 ne LeuM3 PE co 096 175 099 295 CALLA FITC CD10 Anti CALLA FITC 58 0 98 0 34 0 99 32 92 FTTC CD45 FITC 192 0 99 026 1 00 3 78 1 HIV HBV HCV 2 FACS Lysing Solution 10 3 1 2 8
18. PE 585nm BD CellFIX 340181 20 25C 20 25C 10 20 25C 1 5 Ficoll Hypaque Pharmacia BD Vacutainer CPT BD 6 BD FACSFlow 342003 CaliBRITE BD 349502 FACS 8 12x75mm BD Falcon 352052 352054 352058 9 10 Vortex 11 12 FA ANA 13 14
19. rken B Gilks W et al eds Leucocyte Typing IV White Cell Differentiation Antigens New York NY Oxford University Press 1989 800 805 Hanjan SN Kearney JF Cooper MD A monoclonal antibody MMA that identifies a differentiation antigen on human myelomonocytic cells Clin Immunol Immunopathol 1982 23 172 188 Hsu SM Jaffe ES Leu M1 and peanut agglutinin stain the neoplastic cells of Hodgkin s Disease Amer J Clin Path 1984 82 29 Pinkus GS Thomas P Said JW Leu M1 A marker for Reed Sternberg cells in Hodgkin s Disease an immunoperoxidase study of paraffin embedded tissues Am J Pathol 1985 119 244 Link M Warnke R Finlay J et al A single monoclonal antibody identifies T cell lineage of childhood lymphoid malignancies Blood 1983 62 722 728 Palker TJ Scearce RM Hensley LL Ho W Haynes BF Comparison of the CD7 3A1 group of T cell workshop antibodies In Reinherz EL Haynes BF Nadler LM Bernstein ID eds Leukocyte Typing II Human T Lymphocytes New York Springer Verlag 1986 303 313 Weiss LM Crabtree GS Rouse RV Warnke RA Morphologic and immunologic characterization of 50 peripheral T cell lymphomas Am J Pathol 1985 118 316 324 Weiss LM Wood GS Warnke RA Immunophenotypic differences between dermatopathic 3 20 21 22 23 24 25 26 27 28 29 30 31 lymphadenopathy and lymph node involvement in mycosis fungoides Am J Pathol 1985 120
20. 179 185 Picker LF Weiss LM Medeiros LJ Wood GS Warnke RA Immunophenotypic criteria for the diagnosis of non Hodgkin s lymphoma Am J Pathol 1987 128 181 201 Foon KA Todd RF Immunologic classification of leukemia and lymphoma Blood 1986 68 1 31 Wood GS Abel EA Hoppe RT Warnke RA Leu 8 and Leu 9 antigen phenotypes immunologic criteria for the distinction of mycosis fungoides from cutaneous inflammation J Am Acad Dermatol 1986 14 1006 1013 Goyert SM Ferrero E Biochemical analysis of myeloid antigen and cDNA expression of gp55 CD14 In McMichael AJ ed Leucocyte Typing III White Cell Differentiation Antigens New York NY Oxford University Press 1986 613 619 Bernstein ID Self S Joint report of the myeloid section of the Second International Workshop on Human Leukocyte Differentiation Antigens In Reinherz EL Haynes BF Nadler LM Bernstein ID eds Leukocyte Typing Il Human Myeloid and Hematopoietic Cells New York NY Springer Verlag 1986 3 1 25 Jayaram Y Hogg N Surface expression of CD14 molecules on human neutrophils In Knapp W D rken B Gilks W et al eds Leucocyte Typing IV White Cell Differentiation Antigens New York NY Oxford University Press 1989 796 797 Dimitriu Bona A Burmester GR Waters SJ Winchester RJ Human mononuclear phagocyte differentiation antigens I Patterns of antigenic expression on the surface of human monocytes and macrophages defined by monoclonal antibodies J Immunol
21. 1983 130 145 152 Herrmann F Komischke B Odenwald E Ludwig WD Use of monoclonal antibodies as a diagnostic tool in human leukemia I Acute myeloid leukemia and acute phase of chronic myeloid leukemia Blut 1983 47 157 163 Greaves MF Monoclonal antibodies as probes for leukemic heterogeneity and hematopoietic differentiation In Knapp W ed Leukemia Markers New York NY Academic Press 1981 19 Zola H CD10 Workshop Panel report In Schlossman SF Boumsell L Gilks W et al eds Leucocyte Typing V White Cell Differentiation Antigens New York NY Oxford University Press 1995 505 507 Letarte M Vera S Tran R et al Common acute lymphocytic leukemia antigen is identical to neutral endopeptidase J Exp Med 1988 168 1247 1253 Le Bien TW McCormack RT The common acute lymphoblastic leukemia antigen CD10 emancipation from a functional enigma Blood 1989 73 625 635 Nadler LM Korsmeyer SJ Anderson KC et al B cell origin of non T cell acute lymphoblastic leukemia a model for discrete stages of neoplastic and normal pre B cell differentiation J Clin Invest 1984 74 332 340 Hann IM Richards SM Eden OB Hill FGH Analysis of the immunophenotype of children treated on the Medical Research Council United Kingdom Acute Lymphoblastic Leukaemia Trial XI MRC UKALLXI Leukemia 1998 12 1249 1255 Loken MR Shah VO Dattilio KL Civin CI Flow cytometric analysis of human bone marrow II Normal B lymphocyte development Blood 19
22. 87 70 1316 1324 Dworzak MN Fritsch G Fleischer C et al Multiparameter phenotype mapping of normal and post chemotherapy B lymphopoiesis in pediatric bone marrow Leukemia 1997 11 1266 1273 Dworzak MN Fritsch G Fr schl G Printz D Gadner H Four color flow cytometric investigation of terminal deoxynucleotidyl transferase positive lymphoid precursors in pediatric bone marrow CD79a expression precedes CD19 in early B cell ontogeny Blood 1998 92 3203 3209 Schwinzer R Cluster report CD45 CD45R In Knapp W D rken B Gilks WR et al eds Leucocyte Typing IV White Cell Differentiation Antigens New York NY Oxford University Press 1989 628 634 Jackson A Basic phenotyping of lymphocytes selection and testing of reagents and interpretation of data Clin Immunol Newslett 1990 10 43 55 Terstappen LWMM Levin J Bone marrow cell differential counts obtained by multidimensional flow cytometry Blood Cells 1992 18 311 330 Loken MR Brosnan J Bach B Ault K Establishing optimal lymphocyte gates for immunophenotyping by flow cytometry Cytometry 1990 11 453 459 Hermiston ML Xu Z Weiss A CD45 a critical regulator of signaling thresholds in immune cells Annu Rev Immunol 2003 21 107 137 Pellat Deceunynck C Bataille R Normal and malignant human plasma cells proliferation differentiation and expansions in relation to CD45 expression Blood Cells Mol Dis 2004 32 293 301
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