Cycleave®PCR Salmonella Detection Kit Ver. 2.0
Contents
1. 20 V 2 1 v4 4 1 1 20 ul 1 5 2 1 2 x Cycleave Reaction Mixture 12 5 ul Xx SIN Primer Probe Mix 5 X conc 5 ul x or SIN Positive Control or2. DNA PCR DNA PCR DNA DNA dH20 SIN Positive Control jnv4 20 PCR Thermal Cycler Dice Real Time System TP900 TP960 Thermal Cycler Dice Real Time System Lite
3. Run Auto Auto Manual lt gt 5 2 1 2 NC PC NC N gt FAM ROX FAM ROX IC ta m m1 A 4 fm be 3 1 ce TP il PC
4. Thermal Cycler Dice Real Time System 1 CycleavePCR Kit gt 2 CycleavePCR Kit Thermal Cycler Dice Real Time System Software lt PM Plus Minus Assay gt Thermal Cycler Dice i System 2 PCR MM Cycle 95 i 3 step PCR Cycle 45 95 5 55C 10 72 20 3
5. FAM Primer Probe Mix DNA 3 ND http www takara bio co jp 10 CY205 Applied Biosystems 7500 Fast Real Time PCR System Life Technologies 1 Advanced Setup New Experiment 2 Experiment Properties Quantification Standard Curve TaqMan Re agents Other Other Include Melt Curve 1 3 Plate Setup Define Target Target Name SIN Reporter FAM Quencher none 4 Plate Setup Define
6. mv4 FAM ROX 4 DNA PCR Ver 2 0 gt Lot AK701 Smart Cycler System Thermal Cycler Dice Real Time System Applied Biosystems 7500 Fast Real Time PCR System OD Tr NAS 1 http www takara bio co jp 9 CY205
7. Nega ND PCR Error 1 OUT 2 DNA OUT PCR ROX
8. P gt FAM ROX FAM ROX IC i jm 3 1 TT NT 3 gt http wwwr takara bio coJp 9 CY205 6 gt E Fh fakinT lp a tialalalsisir miho sl rl WW ma Mr 1 uk 5 LA UI AI 4 lt iT i CC fii i mm 1 ll 5 m Ln i F Ll ns ri ee 0 1 ix i Ia i i N gt lt lt P gt OK OUT gt Posi
9. 4 5IN Positive Control 5 http wwwr takara bio coJp CY205 VII PCR EEM 225 ml 225 ml 35 18 24 37 18 225 ml 37 18 PCR PCR VIII 1 Saiki R et g 1985 Science 37 170 172 2 1995 16 pp61 3 Galan J E et g 1992 Bacteriol 174 4338 4349 4 Ashok K er al 1994 Microb Pathog 19 85 95 IX Viable Sa monella Selection Kit for PCR 7711 Thermal Cycler Dice Real Time System TP900 TP960 Thermal Cycler Dice Real Time System Lite TP700 TP760 96 well Hi Plate for Real Time
10. Ph 4 3 1 2 3 5 PCR PCR Auto PC Manual V 40
11. 2 PCR 3 U M PCR 4 http wwwr takara bio coJp 5 CY205 V 1 2 1 10 ul 1 5 ml 2 90 ul 3 95 5 4 12 000 rpm 4 10 v4 1 5 u PCR 10 100 PCR
12. CY205 IdRaRa Cycleave PCR Salmonella Detection Kit Ver 2 0 v201406Da sss 3 9 4 I oo 4 NN 4 0 5 oo 5 EL NO YA A 6 V 2 iuiiiiiiassesneerneeeneeseeeeeeeeereeeeseeeneeeeeeeeeeeeeeeeeeeeeeea 6 V 3 PCR Maui 7 Thermal Cycler Dice Real Time System ui 7 Applied Biosystems 7500 Fast Real Time PCR System 11 gt 13 VII PCR ee 14 oo 14 PR 14 15 http wwwr takara bio coJp 2 CY205 2 500 Salmonella Enteritidisv jinvA PCR Hot Start PCR
13. TP700 TP760 Thermal Cycler Dice Real Time System Software Applied Biosystems 7500 Fast Real Time PCR System Life Technologies 95 1 8 0 2 ml Thermal Cycler Dice Real Time System 0 2 ml 8 strip tube individual Flat caps NJ600 http wwwr takara bio coJp 4 CY205 1 000 200 ul 20 10 ul 4 IV 1 PCR 2 3 Mi RG 3
14. 25 ul X 50 1 2XCycleave Reaction Mixture 2 X conc 625 2 SIN Primer Probe Mix FAM ROX 5 X conc 250 ul 3 dH20 1 ml 4 SINPositive Control 150 ul 30 2 x Cycleave Reaction Mixture Buffer dNTP mixture DNA PCR SIN Primer Probe Mix v4 v4 DNA FAM MM ROX DNA v4 DEETT DNA DNA
15. 0 7gg HS 2 RNA DNA RNase H RNase H RNA 1
16. 1 ii is ee Internal Control ROX 10 View Well Table rai ri ET EL PPT PFFT PFTT RF 1 LILI StepOnePlus Real Time PCR System Life Technologies ROX target ROX IC FAM ROX target http wwwr takara bio coJp 12 CY205 VI 1 ho pN invA invA nvA a inv4 lt 3 2 SIN Positive Con
17. SD dH20 25 Total 25 Wl SIN Positive Control 2 3 2 3 SIN Positive Control PCR 1 http wwwr takara bio coJp 6 CY205 V 3 PCR 3 PCR 3 RY Thermal Cycler Dice Real Time a 7 7500 Fast Real Time PCR System Life Technologies
18. NJ400 Sealing Film for Real Time NJ500 Plate Sealing Pads 9090 0 2 ml Hi 8 Tube NJ300 0 2 ml Hi 8 Flat Cap NJ302 0 2 ml 8 strip tube individual Flat Caps NJ600 http wwwr takara bio coJp 14 CY205 Cycleave Thermal Cycler Dice 7aKaRa Ex 7ag 4 http wwwr takara bio coJ
19. Target Target Name IC Reporter ROX Quencher none 5 Define Samples NC PC 6 3 4 5 Plate Layout Passive Reference none 7 Instrument a Vnlumm Pai mw mm up lods Hold HaidegSiaen 8 Org hm Cycle 1 Tr 95 10 lgmWTH CI I 3 step PCR Cycle 45 19N mm 95 5 55 C 10 0 Bt 72 25 a P 90 8 Start 9 Analysis Amplification Plot Target Threshold Baseline Manual SIN Target FAM http www takara bio co jp 11 CY205 Le ee Tr es i Mf L Ls mr EE I EE T rp 1 LS i 3 a Em 1 i
20. http wwwr takara bio coJp 7 CY205 4 Omit mi 9 es PHT Pr Es _ KO F nr TF EE WW ROX 2 5 1 i re EE re i FT Fe 1 Fm 1 i Fj i 1 1 g mi 4 mm OT TT TT TT FET TT TE TI LE LE L JI i 1 WW a T Py 1 ff FT a i a i a TI jn i pem i i re a 5 1 1 _ ra tk a a LL 2 FAM C ROX http www takara bio coJp 8 CY205
21. p 15 CY205 sm Iaf4R4 Tel 077 543 6116 Fax 077 543 1977 http www takara bio coJjp v201406Da
22. trol 0 4 aha DN Mi inv4 inv4 74 linv4 4 3 3 ho pN invA invA jnvA a nvA 9 0 Se 3 1 DNA 4 2 3 PCR
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