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Contents
1. 4 pp Tel 06 6348 3888 9 00 128 00 13 00 1 7 00 tech_osaka tOyODOJ jp NEV PCR EHEC PCR EvaGreen PCH PCR 12. PCR 15 PCRH 50 3 1 90 95 PCR 90 95 50 PCR 10 1
3. Uracil DNA Glycosylase 10310 PCR 3 DNA DNA DNA 100 10 PCR Bio Rad MiniOpticon 100 10 Fi i EE 1 i 1 Temperature Celsius Termperature Celsius PCR Master Mix 10XPrimer Mix UNG Uracil DNA Glycosylase PCR
4. Code No FIK 203 WGI GII 5000 500 500 500 Gz y 1 el Gi G17G2 GU 1 1 1 Mt 1 mW 1 1 4 1 1 ir i Ci nasaaaseaa 1 i 1 I 5 A 1 1 1 T 1 1 WT 1 1 iu he 5 aE a 1 i 1 NG14G2 Bio Rad MiniOpticon G1 G1 1 FIK 20 1 75 000 EvaGreen dye 20X in water G G o0 FIK 202 75 000 EvaGreen dye 20X in water G1 G G1 G 1 FIK 203 80 000 EvaGreen dye 20X in water EvaGreen Biotium Inc Bio Rad MiniOpticon Bio Rad Laboratories Inc
5. 1 step RT PCR 1 step HT PCR EvaGreen ReverTra Ace rTaq DNA Polymerase 1 step RHT PCR step RT PCR 8 30 1 step RHT PCR RT PCH PCR 30
6. THUNDERBIRD qPCR Mix Code No QPS 101 QPS 201 FNA direct Realtime PCR Master Mix Code No QRHT 101 QRHT 201 1 step HT PCHR SuperPrep Cell Lysis amp RT Kit for PCR HEK293 5X 30 10 cells cDNA 40l HEK293 Total RNA 66 6ng ReverTra Ace qPCR RT Master Mix Code R 0 983 No FSQ 20 1 cDNA 40 cDNA THUNDERBIRD Y SYBR Y_ qPCR Mix Code No QPS 201 15 PCR Ct 15 RNA 10
7. 1 PCR G 1 75 5C 78 5C G I 78 5C 81 0C GI GII Ei EE G1 Code No FIK 201 G2 Code No FIK 202 G 1 GI RNA 1 step RT PCR RNA 50 Gl Gl LT jn hs ll i pl i f Tr ef 6250 1 ke we 3 et 1250 A ee i lt A et OO WW 9 EH 4 N a 1 L ed er e ab N i 2 Bio Rad MiniOpticon GI GIHI
8. PCR KOD FX Neo PCRH KOD FX Neo PCR KOD FX Neo PCR KOD FX Neo KOD FX Neo S00Ux1 200 P0C KFX 0 1 35 000 KOD FX Neo 1 0U ul XPCR Buffer for KUD FX Neo 200UX1 X5 1 000 0 KFX 201X5 140 000 mM dNTP 200UX1 X10 2 000 20 KFX 201X10 260 000 50I 901JKe7 8 2014 102
9. KOD SYBR qPCR Mix GC KOD SYBR qPCR Mix KOD SYBR qPCR Mix 1mlX1 40 QKD 201T 9 800 KOD SYBR qPCR Mix 50xROX reference dye 1 67mlx3 200 QKD 20 1 000 1 67mIlX3 5 1 000 QKD 20 1X 5 147 000 50xROX reference dye 50I KOD SYBR qPCR Mix SYBR Applied Biosystems Life Technologies Corporation illumina MiSeq TruSeq illumina Inc KOD FX Neo PCR PCR 1
10. SuperPrep Cell Lysis amp RT Kit for qPCR Code No SCQ 101 cDNA cDNA THUNDERBIRD Probe qPCR Mix Code No QPS 101 actin IL 8 TNF TaqMan 01JKe7 2014 102 3 1 8 actin Ct Ct
11. 10 cells DNA Hemover Lysis Solution gDNA Remover Lysis Solution gDNA Remover Lysis Solution RINase RNase RT cDNA 2 20 40lI 096 40l 8l cDNA qPCRH cDNA qPCR
12. RT PCR 1 step RT PCR 2 30 EvaGreen PCR 85C 1 RT PCR 50 1 step HT PCR GI GI GI GI 3 3
13. 4 illumina illumina MiSeq A B Read 1 Read 1 a Dencity Cluster PF Phas Prephas Reads el ES Dencity ClusterPF Phas Prephas Reads mm2 M K mm2 M 1 38 1059 19 95 13 0 43 0 182 0 078 26 07 1 38 1157 33 93 89 0 73 0 161 0 092 28 32 Read 2 Read 2 Lane Ti Dencity Cluster PF Phas Prephas Reads Lanel Tl Dencity ClusterPF Phas Prephas Reads 0 M WW2 M 1 38 1059 19 95 13 0 43 0 186 0 073 26 07 1 38 1157 33 93 89 0 73 0 154 0 071 28 32 KOD SYBR qPCR Mix A B NGS Density Cluster PF 800 1300k mm 8096 KOD SYBR qPCR Mix NGS
14. PCR PCRH DNA PCR 1 PCR Tm 015
15. Chrome9 1kb MSH6 1 8kb BRCA2 2 25kb WT 1 2 5kb FANCE 3kb RAD51D 4kb KRAS 5kb BRCAT 7kb DDB2 10kb PCR DW 7 PCR PCR xBuffer for KOD FX Neo 25 Ll 98C min 98C min mM dNTPs 10 Al Primer mix primer 2 5 pmol ul 6 ll 98C 10sec 98C 10sec Ce 50 ng ul Human genomic DNA 1 ul 60C 30sec 30 cycles 68C 10min KOD FX Neo T al 68C Tmin total 50 ul 4C 4C Ke7 2014 102 7 PCR PCR 1 000bpp 10kbp 5kbp gt 500bpp 1kb Ladder KOD FX Neo M 100bp Ladder A Taq PCR 1 KOD FX Neo B Long Range PCR 0 2 A Tag PCR 0 B PCR 3 B PCR C PCR 4 B Multiplex PCR B Multiplex PCR PCR KOD FX Neo PCR KOD FX Neo 7kb 10kb
16. KOD FX Neo PCHRH PCRH KOD FX Neo PCR PCRH PCR 50ng ul Human genomic DNA 5 I PCH PCR PCR 1 000bp 10kbp 5kbp gt 500bpp B PCR B Multiplex PCR C PCR B Multiplex PCH M 1kb Ladder 5 KOD FX Neo M 100bp Ladder 6 A Taq PCR 100bpp 1 KOD FX Neo 7 B Long Range PCR 2 A Taq PCR 1kbp 8 B PCR 3 9 4 0 KOD FX Neo
17. 20 Eg RHNase HNA RNA DNase I cDNA DNA cDNA ReverTra Ace cDNA cDNA DNase1l DNase I Stop Solution RHNA
18. 2 4 LOG cell number 4 amp 2 86 PMA U937 PCR so actin Ct 20 IL 8 TNF PMA 20 U937 RNase U937
19. G I GI G I GI G I GI RNA 5 000 500 500 500 500 5 000 G1 Code No FIK 201 G2 Code No FIK 202 G1 G2 Code No FIK 203 G 1 GI G 1 G2S RNA G I GI G1 G2 G 1 GI G 1 GI 1 Code No FIK 20 1 202 Code No FIK 203
20. 4 20pM ly 20pM 20pM 03125py 03125pM 033125pM li i li 901JKe7 2014 102 RNA seq 4pM RNA seq A G60Dp A PCH Taq KOD SYBR qPCR Mix B NGS imi tr gmhmnm FT i dC mm i FEELmP_ mn s et 4 1 RNA seq DNA KOD SYBR qPCR Mix A PCHR PCH 200bp 860bp Taq PCR
21. PCR PCR PCR PCR KOD FX Neo KOD FX Neo PCRH PCR KOD FX Neo PCR
22. 4 Reading frame ORF 5 EXON OPEN ONLY CLOSE OPERON CODING INTRON CISTRON PROMOTER READING TELOMERE REPRESSOR HAPLOTYPE SATELLITE CENTROMERE UPLOAD 10 1 et 2 13 www toyobo co jp bio 2014 9 2 2014 11 3 _ httD www toyobo co jp seihin xr lifesc
23. PCRH DW 92 95C 10 min KOD SYBR qPCR Mix 104 1Opmol ul Primer 1 0 4 95C 10 sec 60C 30 sec x40cycles 10pmol ul Primer 2 0 44 50XRHOX reference dye 0 04 Melting DNA YA Data collectlon total 0 DNA 20pM 0 196Tween 0 Applied Biosystems 7500 Fast MuItilNA DNA RNA DNA 4pM dPCR PCH PCR PCH ROX 1 DNA A PCR Taq KOD SYBR qPCR Mix B NGS
24. MATREX LDM TMLDM 001 2 015 17 2015 3 9 TESTSKIN LSE high 1 TMLSE 013 2 015 17 2015 3 9 TESTSKIN LSE high 1 A L TMLSE 013A 2015 2 17 2015 3 9 TESTSKIN LSE high TMLSE 003 2015 17 2015 3 16 TESTSKIN LSE high A L TMLSE 003A 2015 17 2015 3 16 http www toyobo co jp seihin xr lifescience info index html UPLOAD Vol 10 1 http Www toyODO CO jD Seihin xr Iifescience info index html Label License Label License Statement NOTICE TO PURCHASER
25. 90 95 10 1 3 1 1 MMKe7 2014 102 ge HL 7 EE EvaGreen Biotium Inc EvaGreen PCR 1
26. G 1 Code No 6250 me FIK 201 250 0 G2 Code No FIK 202 GI GII RNA RNA 50 1250 p 9 10 gt 1 mRNA DNA sequence CDS ea 3 1 mRNA
27. PCR 1kb PCR KOD FX Neo 1kb 10kb PCR PCR DMD BMD PCRH KOD FX Neo PCRH PCR PCR Alan H Beggs et al Human Genetics 1990 86 45 48 PmF 535bp Exon3 410bp Exon6 202bp Exon13 238bp Exon43 357bp Exon47 181bp Exon49 439Dp Exon50 271bp Exon60 139bp PCR PCR 1kb 10kb PCR KOD FX Neo PCR PCR PCR
28. SuperPrep Cell Lysis amp RT Kit for qPCR PCR cDNA SuperPrep Cell Lysis amp RT Kit for qPCR Code SCQ 101 PCRH Lysis Reagents RT Reagents 96 RNA RT PCR cDNA SuperPrep Cell Lysis Kit for qPCR Code SCQ 201 Lysis Reagents 1 step RT PCR Lysis R
29. Total RNA C1 20 A 15 10 15 20 25 30 C1 Supe P ep U337 RNase U937 1xX104 1x103 1x105 1x10cells 8 cDNA 40 THUNDERBIRD SYBR qPCR Mix Code No QPS 20 1 g actin PCR 0 A PCHR A Total RNA 6 ReverTra Ace qPCR RT Master Mix Code No FSQ 201 THUNDERBIRD SYBR qPCR Mix Code No QPS 201 HNA SS Supe Prepe A RNNA SuperPrep Cell Lysis amp RT Kit for qPCR Lysis Reagents lt RT
30. THUNDERBIRD Probe qPCR Mix Code No QPS 101 g actin TaqMan rpmx5min PBS 100 86 U9837 5X10 cells ml 109 FBS RPMI PBS 1640 d6 2ml 0 0 01 0 1 1 10 M Phorbol 12 myristate 13 acetate PMA 37C C0 6 SuperPrep Cell Lysis amp RT Kit for qPCR 36 100 3 5 1 10 cells 86 2 000 rpm 5 PBS 100 2 000rpm 5 PBS
31. KOD DNA polymerase KOD SYBR Green I PCR 3 5 KOD exo DNA polymerase KOD 809 GC kb PCR illumina 1 RNA seq A D total RNA TruSed Standard mRNA illumina DNA PhiX Control Kit v3 illumina 375bp 1 RNA sed Primer 1 AAT GAT ACG GCG ACC ACC GAG AT 23bp Primer 2 CAA GCA GAA GAC GGC ATA CGA 1DDp
32. 96 1x10 1x10 1Xx103 1 Xx10 1 x 105cells 100 ul 3 3 2 000rpm 5 3 PBS 100 2 000rpm 5 96 3 lt gt PBS DO SuperPrep Cell Lysis amp RT Kit for qPCR Code No SCQ 101 A cDNA cDNA
33. PMA SuperPrep Cell Lysis amp RT Kit for qPCR Code No SCQ 101 TaqMan Roche Molecular Systems Inc 1JKe7 4 2014 102 PCR KOD SYBR qPCR Mix NGS PCR 200Dbp PCHR KOD SYBR qPCR Mix
34. LIMITED LICENSE This product is provided under an Agreement between Biotium and TOYOBO The manufacture use sale offer for sale or Import of this product is subject to one or more patents or pending applications owned or licensed by Biotium Inc U S Patent Nos US7 803 943 B2 US7 776 567 B2 Japan Patent No 5249013 The purchase price of this product includes a limited non transferable immunity from suit under U S Patent Nos US7 803 943 B2 US7 776 567 B2 Japan Patent No 5249013 and corresponding patent claims outside of the United States to use only the purchased amount of the product and components of the product solely for the buyer s own internal research whether the buyer is an academic or for profit entity in a manner consistent with the accompanying product literature No right to use sell or otherwise transfer a this product b its components or c materials made using this product or its components to a third party or otherwise use this Droduct or its components or materials made using this product or its components for any other Durpose or a Commercial Purpose IS hereby granted expressly by implication or by estoppel Commercial Purpose means any activity by a party for consideration and include but is not limited to 1 use of the product or itS components in manufacturing 2 use of the product or its components to provide a service Including without limitation reporting information or data resulting from use of the
35. 480 50 24 000 480 20 FIK 301 40 000 EvaGreen Biotium Inc Bio Rad MiniOpticon Bio Rad Laboratories Inc MKe7 12 2014 102 PCR amp cDNA SuperPrep Cell Lysis amp RT Kit for qPCR am 1 A431 H sapiens epidermoid carcinoma cell line 2 CsOne M musculus myoblast cell line 3 Caco H sapiens colon adenocarcinoma cell line 4 CHO K1T C griseus ovary cell line 5 COLO205 H sapiens colon adenocarcinoma cell line 6 DLD 1 H sapiens colon adenocarcinoma cell line 7 HCT 15 H sapiens colon adenocarcinoma cell line 8 HDF H sapiens primary foreskin fibroblasts primary cell 9 HEK293
36. H sapiens embryonic kidney cell line 10 HeLa S3 H sapiens cervix carcinoma cell line 11 HepG H sapiens hepatocellular carcinoma cell line 12 _ HUVEC H sapiens umbilical vein endothelial cells primary cell 13 Jurkat H sapiens T lymphocyte cell line T 14 K562 H sapiens myelogenous leukemia cell line 15 KUSA A1 M musculus bone marrow stromal stem cell line 16 L929 M musculus aneuploid fibrosarcoma cell line 17 MCF 7 H sapiens breast adenocarcinoma cell line 18 Neuro2a M musculus neuroblastoma cell line 19 NIH 3T3 M musculus embryo fibroblast cell line 20 PC1 8 R norvegicus adrenal pheochromocytoma cell line 1 rMSC R norvegicus bone marrow stromal stem cells primary cell 22 THP 1 H sapiens acute monocytic leukemia cell line 23 U937 H sapiens leukemic monocyte lymphoma cell line 54 HH Sprague dawley hepatocyte primary cell 86 1 1X10 7x10 cells
37. product or a itS components for a fee or other commercial consideration 3 use of the product or its components for therapeutic diagnostic or prophylactic DurposeS Or 4 resale of the product or its components whether or not such product Or its components are resold for use in research For products that are subject to multiple limited use label licenses the most restrictive terms apply This product is for research use only Diagnostic uses require a Separate license from Biotium Inc For information on purchasing a license to this product including for purposes other than research contact Director of Licensing Biotium Inc 3159 Corporate Place Hayward CA 94545 Tel 1 510 265 1027 Fax 1 510 265 1352 Emalil btinfo biotium com All names containing the designation are trademarks of Biotium 01JKe7 14 2014 102 1 step RHT PCR 2 EvaGreen Gll
38. 10 15 qPCR 10 qPCR cDNA 7 25 30 i NN 13 TESTSKIN MATREX
39. Reagents gt 100 P0C SCQ 101 6 000 Lysis Solution 5 x RT Master Mix StOD Solution 5 x RT Master Mix no RHT control gDNA Remover Nuclease free Water 100 X5 P0 SCQ 101X5 RNase InhiDitor 5 000 SuperPrep THUNDERBIRD Probe qPCR set 3 SCQ101 SuperPrep Cell Lysis amp RT Kit for qPCR SCQ 101 20 PS101 THUNDERBIRD Probe qPCR Mix QPS 101 Q SuperPrep THUNDERBIRD SYBR qPCR set SuperPrep Cell Lysis amp RT Kit for qPCR SCQ 101 0 SCQTOT THUNDERBIRD SYBR qPCR Mix QPS 201 QPS201 99 500 99 500 SuperPrep Cell Lysis Kit for qPCR S Lysis Solution gDNA Hemover SCQ 20 1 StOD Solution RHNase InhiDitor 40l 100 50 200 20 500 39 000 3 SuperPrep Cell Lysis amp RT Kit for qPCR 100 76 000 72 270 THUNDERBIRD Probe qPCR Mix Code No QPS 101 200 29 000 27 230 4 SuperPrep Cell Lysis amp RT Kit for qPCR 100 76 000 72 450 THUNDERBIRD SYBR qPCR Mix Code No QPS 201 200 29 000 27 230 5 SuperPre
40. eagents Lysis Solution Stop Solution Lysis Solution Stop Solution gDNA Remover gDNA Remover RNase Inhibitor step RHT PCR RNase Inhibitor 50 10l Lysis Reagents Supe P e p Gel Lysis Kit for SCG 201 Lysis Reagents RT Reagents RT Reagents PCR LL 5xRT Master Mix 5xRT Master Mix no RT control Nuclease free Water 1 step RT PCR SuperPrep Cell Lysis amp RT Kit for qPCR PBS Code No SCQ 101 PBS Lysis Solution gDNA Remover 5 DNA Stop Solution
41. ience tech reader quiz index html Ideas amp Chemistry 530 8230 2 TEL 06 6348 3786 FAX 06 6348 38393 E mail order lifescience toyobo jp 104 8345 17 10 TEL 03 6887 8819 FAX 03 6887 895 1 E mail order lifescience toyobo jp TEL 06 6348 3888 FAX 06 6348 3833 9 00 12 00 13 00 17 00 E mail tech_osaka toyODO jp URL http www toyobo co jp bio x A5077K 2014 9 22 000 1
42. ldeas amp Chemistry HOT ITEM PCR amp cDNA SuperPrep Cell Lysis amp RT Kit for qPCR TECHNICAL REVIEW SuperPrep Cell Lysis amp RT Kit for qPCR 96 SO eo Cell Lysis amp RT Kit for qPCR p 1 4 PCR KOD SYBR qPCR Mix KOD FX Neo PCR NEW RELEASE Q amp A PCR amp cDNA SuperPrep Cell Lysis amp RT Kit for qPCR INFORMATION _ TESTSKINT M MATREX Label License HOT PCR amp cDNA
43. p Cell Lysis amp RT Kit for qPCR Lysis Reagents SYBR Life Technologies Corporation TaqMan Roche Molecular Systems Inc 2 Ke7 2014 102 SuperPrep Cell Lysis amp RT Kit for qPCR 96 96 6 SuperPrep Cell Lysis amp RT Kit for qPCR U9837 1 U937 1096FBS RPMI 1640
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