Cell-able Oncology
Contents
1. 5 6 4 6 Ver1 02 Transparent Inc www cell able com TEL 81 476 98 3471 E mail info transparent co jp TRP 7 1 ITH 0 5 2 0 10 6 0 5 2X 105 ml 96 384 10ml Plate type dispense volume well 384well plate 25ul 96well plate Regular area2. 3 270 1609 4 2 1 e mail info transparent co jp D 8 Ver1 02 Transparent Inc wwwr cellable com TEL 81 476 98 3471 E mail info transparent co jp
3. H 5 1 1 www cell able com TEL 81 476 98 3471 E mail info transparent co jp TRP 1 2 384 96 1 3 1 0 5 2 0X106 4 CO f VF aN A Cell able plate
4. 100ul 96well plate Half area 50ul COs 12 Eh DOREM 24 2 1 2 vy 7 Ver1 02 Transparent Inc www cell able com TEL 81 476 98 3471 E mail info transparent co jp TRP
5. of the pipette does not come into contact with the well bottom and then add the new culture medium gently Be sure to leave approx 20 30 of the old medium to prevent the spheroids from drying out Disposal Plates chips and other items used in the experiments must be autoclaved and then disposed of in accordance with your local government regulations 3 Ver 1 02 Transparent Inc www cell able com TEL 81 476 98 3471 E mail info transparent co jp TRP This product is intended for research purposes only and is not to be used for clinical diagnosis This Precautions for use of Cell able Oncology plate product is not for application to humans or laboratory animals Transparent Inc is not liable for any damages that may result from the use of Cell able Oncology plate Manufacturer Transparent Inc 4 2 1 Wakahagi inzai Chiba 270 1609 Japan E mail info transparent co jp COSMO BIO CO LTD Inspiration for Life Science 4 Ver 1 02 Transparent Inc www cell able com TEL 81 476 98 3471 E mail info transparent co jp D TRP Cell able Oncology Ver1 02 Transparent Inc Cell
6. COSMO BIO CO LTD TRP Inspiration for Life Science Cell able Oncology Culture plate for tumor cells in 3D Instructions for use Cell Note This kit is intended for research purposes only and is not to be used for clinical diagnosis This product is not for application to humans or laboratory animals Please read the instructions before using the kit 1 Ver 1 02 Transparent Inc www cell able com TEL 81 476 98 3471 E mail info transparent co jp TRP This product enables to culture tumor cells not only cell lines but patient derived primary tumor cells in Introduction spheroid state Depending on the condition of the Tumor cell used and various other factors the quality of cultured tumor cell spheroid and the experimental results may vary in certain cases When using this product please wear protective laboratory clothing disposable gloves and safety goggles 2 Components Cell able Oncology plate Instruction for Use this document 3 Requisite cells Approx 0 5 2x10 cells plate of Tumor cells 4 Requisite apparatus and devices CO incubator Clean bench Centrifuge Other devices such as a pipette micropipette chip and centrifuging tubes as needed Be sure to use sterilized devices 5 Requisite reagents culture grade Appropriate culture medium for the tumor cell used Follow the information of the Tumor cell you use 6 Storage condition and st
7. ability Storage condition Store at 2 8 C Stability indicated on a label of product 2 Ver 1 02 Transparent Inc www cell able com TEL 81 476 98 3471 E mail info transparent co jp 7 Procedure for culturing cells Follow the steps described below for culturing cells in the clean bench Seeding the tumor cells Y Prepare cell suspension with culture medium to 0 5 2 0x 10 cells ml of cell density Y Seed the suspended cells in the wells of the Cell able Oncology plate according to the following table taking care to disperse the cells equally The plate must be placed in the incubator immediately after seeding the cells Plate type dispense volume well 384well plate 25ul 96well plate Regular area 100ul 96well plate Half area 50ul v Initiate incubation in the CO incubator Leave the plate in the incubator for 24 hours after seeding the cells Be careful not to apply vibration or shock Example schedule for culture medium replacement Monday Tuesday Wednesday Thursday Friday Saturday Sunday Monday Replacement Replacement Replacement Replacement Precautions for culture medium replacement In early date after seeding poor spheroid agglutination may result in cells peeling away from the plate if subjected to a strong shock When replacing the culture medium remove the old medium carefully so that the tip
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