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1. 4 No Ri I I MWS 1000A 246360 246370 1 2 0 5 10mL 0 5 5mL 1 10mL 3 10 70C 0 50 500W 0 1300rpm 4 5mm2. 1 RT LED No 246410 150 000 9g 0120 076 554 EYELA lt MWS 1000A
3. Hhttp wwwr eyela cojp products mws1000 reaction shtml Fmoc nw AcyI Carrier Protein 65 74 10 GM 1 15 H Val GIn Ala Ala lle ASsp Tyr lle Asn Gly OH H Val Trp Arg Leu Leu Ala Pro Pro Phe Ser Asn Arg Leu Leu Pro OH MWS 1000A 50C Resin Wang 100mg 50mg gt FmocAA 3eq HBTU 3eq FmocAA 5eg X2 DIC 5ed X2 MWS 1000A 50C Resin 2 chlorotrityl 80mg 30mgx4 HOBt 3eq DIEA 6ed HOBt 5eqg X2 DIEA 6ed X2 FmocAA 3eq H
4. USB 2450 30MHz 500W 10 30 C mm 565Wx430Dx536H 39kg 15A AC100V 50 60Hz 6 000 000 5 300 000 700 000 2 MWS 1000B No 246360 246380 UC 2 3 1 1 20mL 10 200C 0 50 500W 100 1300rpm 1 3kgf cm
5. USB 2450 30MHz 500W 10 30 C mm 565Wx430Dx536H 39kg 15A AC100V 50 60Hz 5 550 000 5 300 000 250 000 2 EVELA http www eyela co jp 2 MWS 1000A _ F 0 7 5 5 ling 1 line2 line3 line4 2 NM L_ 1
6. 1 3 a gt 1 USB WS 7000 xe a gg 100V Max 2A N0 183500 100 246390 5 246400 1 131400 5 16 500 10 10 000 5 24 000 2 1 11 000 2 0 5 5mL PP 1 10mL PP PFA 10um 234890 1 1 800 1 N 60 100 P ne
7. 20 15minX1 60minx2 10min 1 10minX1 Fmoc 10minx1 30minx1 5minX1 5minX1 6h 20h 2 5h 2 5h ca 150mL ca 300mL ca 150mL ca 150mL 2h 30min H Tyr ACPC R Abu ACPC S OH H Tyr ACPC S Abu ACPC R OH H Tyr ACPC S Abu ACPC S OH HO HO 1 1 LN N AA 22 U ww tl HaN NT NN mr NE NTT NL 0 0 0 O 1 10 80 MeOH HzO 0 1 TFA 35min 2 10 80 MeOH HzO 0 1 TFA 35min 3 10 80 MeOH HzO 0 1 TFA 35min a L 1 3 250 3 000 gool EE goof 0 200 800 600 150 600 500 oo 500 400 sl 0 50 300 200 200 100 0 100 50 0 100 100 0 5 10 15 20 25 30 35 40 45 50 0 5 10 15 20 25 30 35 40 45 50 0 5 10 15 20 25 30 35 40 45 50 min min min NN 2 gg 0120 076 554 EYELA
8. A 0 8 750 06 3000 500 0 4 2000 250 0 2 1000 0 0 0 1 1 1 1 1 1 1 1 1 1 2000 2500 3000 3500 4000 0 4 8 12 16 20 24 28 m z min EVELA http www eyela co jp 2750 0 5 m z ASS 3172 532 10 15 20 25 30 35 40 min 0 1 1500 2000 2500 3000 3500 4000 4500 0 5 m z 15 20 25 30 35 40 min 10 2 50mg 16 H Asm 1 Asm V OHAsm A Asm V S V N MezMet N G alloT OH MWS 1000A 50C Resin 2 chlorotrityl 20mg 450mg FmocAA 4eq HBTU 5ed HOBt 5eq DIEA 10eq FmocAA 4eq HATU 5eq HOAt 5eq DIEA 10eq 9 H QQQQQQQQQ OH MWS 1000A 50C Resin 2 chlorotrityl 200mg 200mg FmocAA 3eq HBTU Sed FmocAA 4eq HBTU 5ed HOBt 3eq DIEA 6eq HOBt 5eq DIEA 10eq NMP 10mi
9. BTU Sed FmocAA 4eq x2 DIC 4ed X2 0 06 0 04 0 02 0 00 DMF NMP HOBt 3eq DIEA 6eq HOBt 3 5eq X2 DIEA 6eq X2 10minX1 45minx2 DMF NMP DMF Fmoc 3minX1 3minx1 20minx1 10minX1 40 minX2 5h 48h Fmoc 3minx1 5minx1 15minx1 72 61mg 60 5h 4 ca 370mL ca 1 000mL 52 63mg 50 72mg ca 530mL N Fujitani H Shimizu T Matsubara T Ohta Y Komata N Miura T SatO S l Nishimura Carbohydr Res Special lssue Glycomimetics 342 1895 1903 2007 MWS 1000A MWS 1000A 5 ES x x 2 NR 8 3 20 8 E 0 a 500 0 2 4 6 8 10 12 14 16 18 20 22 24 26 min 400 600 800 1000 1200 1400 1600 m z Polygalacturonase C 30 H IEEMPAQDPNDPEDPDTAMQEAEAEEAAAG OH 44 H ADQPASNDIEEMPAQDPNDPEDPDTAMQEAEAEEAAAGNSTTSG OH after Durification_HPLC crude_HPLC crude_MASS after Durification_M 5 1 2 5000 1250 1000
10. For solid phase peptide synthesizing with microwaVe gt ve Magic Microwave Synthesizer MWS 1000A 1000B MWS 1000A 4 a Nn OO Excel Oo 0S Windows XP Vista 7
11. Microsoft Excel MWS 1000B MWS 1000A 1000B MWS 1000B 10 70 0 50 500W PP 0 5 5mL 0 5 10mL
12. nx1 8 3minx1 2 10minx1 8 3minx1 Fmoc 3minX1 3minx1 Fmoc 3minx1 3minx1 8h 18h 13h 22 91 8mg 15 5 21 2 3mg 3 7 6 3mg ca 300mL ca 600mL ca 500mL ca 1 000mL 8B ACPC 4 H Tyr ACPC S H Tyr ACPC S H Tyr ACPC R Abu ACPC S OH Abu ACPC R OH Abu ACPC S OH MWS 1000A 50 0 65mmol g 0 13mmol FmocAA 2eq HBTU 2ed HOBt 2eq DIPEA 4ed MWS 1000A 50C 0 65mmol g 0 13mmol FmocAA 2eq HBTU 2eq HOBt 2eq DIPEA 4ed MWS 1000A 50 0 65mmol g 0 13mmol FmocAA 2eq HBTU 2ed HOBt 2eq DIPEA 4ed 450mg FmocAA 2eq HBTU 2ed HOBt 2eq DIPEA 4ed Resin Fmoc NH SAL DMF DMF DMF
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