MORA-EXTRACT
Contents
1. 2 SDS DEPC 2 10 C RNA RNA DNA2. 121C 30 S1 Advanced Microorganism Research 69505KEFO1I 9 2 10 C 10 9 103 0024 7 8 03 5645 5664 AM 501 1111 2 210 1 058 293 0610 FAX 058 234 2487 92
3. 150u L 1 70 90C 10 RNA 70 C DNA 70C 90 C Scientific Industries 2 Vortex2 DNA RNA 200u L SDS 70 C10 400u L 2 1
4. 3 70 90 C 10 1 RNA 70C DNA 70 90 4 Scientific Industries 2 Vortex2 DNA RNA 2 5 SDS 200u L 70 C 10 SDS
5. RNA RNA RNase DNA RNA 3 429 409 6 2 0 729
6. 6 Advanced Microorganism Research 692502 7 2 RNA 1mL 2mL 90 4 C 3 15 000 rpm 20 47C 4 200u L 1 200u L SDS 70 C 10 5 400 u L 2 1 Vortex 15000 rpm 3 RNA DNA RNA 1 RNA 200 u L 2mL 99 1000u L vortex 15000 rpm 3 2 70
7. 1 2mL 2mL 15000rpm 3 2 150u L 1 3 70 90C 10 RNA 70 DNA 70 90C 4 Scientific Industries 2 Vortex2 DNA RNA 5 200u L SDS 70 10 6 400u L 2 1 Vortex 15000 rpm 3 DNA
8. 5 70 90C 10 RNA 70 DNA 70C 90 6 Scientific Industries 2 vortex2 DNA RNA 7 200u L SDS 70 C10 8 400u L 2 1 Vortex 15000 rpm 3 DNA DNA RNA 1 DNA 200 pL 2mL 99 1000u L vortex 15000 rpm 3
9. 20 Advanced Microorganism Research 69505KEFO1I F DNA RNA 10 DNA RNA F 1 1 2mL 2nL 15000rpm 3 2 1 150u L 70C 10 3 Beads Scientific Industries 2 vortex2 DNA RNA 4 200u L SDS 70 10 5 400 un L 1 Vortex 15000 rpm 3 DNA F 2
10. 1 2 MSDS 8 Advanced Microorganism Research 69503KFO1I B 2 DNA RNA Sml 1mL 200 mL 1 Vortex 20 200 L 200 SDS 400L gt 70 90 C i 3 Vortex 15 000rpm 1 3 99 70 1000L a 1000gL 15 000rpm 15 000rpm Vortex 3 Vortex 3 DEPC T10B1 DNA RNA WW 9 Ad
11. 25 Advanced Microorganism Research 69505KEFO1I D DNA RNA 103 109 cfu g 4g Falcon 50 ml Tube 35ml buffer 125mM NaCI 50mM EDTA PH 8 0 1ml 20 skim milk Vortex Silica DNA PCR 5 10 Phenol 2 15 1 2ml 2mL 15000 rpm 5 2ml 150 u L 1 2 70 90 10
12. RNA 70C DNA 70C 90C 3 Scientific Industries 2 Vortex2 DNA RNA 4 200u L SDS 70 10 5 400 L 2 1 Vortex 15000 rpm 3 DNA 100u L 200u L step5 DNA RNA 1 DNA 200 u L 2mL 99 1000u L vortex
13. 6 400u L 15 000 rpm 3 2mL 3 4 7 99 1mL 15 000rpm 3 70 1mL 15 000rpm 3 5 8 200u L DEPC
14. 2 70 C 10 RNA 70C DNA 70C 90C 3 Scientific Industries 2 Vortex2 DNA RNA 4 200u L SDS 70 10 5 400u L 2 1 Vortex 15000 rpm 3 DNA DNA RNA 1 DNA 200 u L 2mL 99 1000u L vortex 15000 rpm 3 2 70
15. 22 Advanced Microorganism Research 69503KFO1I G DNA RNA 200 uuL ibm 1 1 200p L 100u L 1 2 70 90C 10 RNA 70C DNA 70 C 90C 3 Scientific Industries 2 vortex2 DNA RNA 4 200u L SDS 70 C10
16. 2 70 1000u L 15000 rpm 3 DNA RNA 70 99 DEPC 3 200p L DEPC Option DNA RNA DNA 2u L NanoDrop 260nm 260nm 280nm 260nm 230nm 1 2 MSDS 24 Advanced Microorganism Research 69505KEFO1I H 2 DNA RNA a 15mL 100 LL B 4000x g B gt 100 L aml 1 15mL
17. 1 0 859 6 NaCl 90mL 10g 2 5 3 2mL 1 F 3 2mL 15 000 rpm 3 A 10g 90mL peptone 1000mL 1g peptone 5 2mL 1 1 Advanced Microorganism Research 69505KEFO1I F 4 90mL 1 34g 500mL 1N NaOH 175mL pH7 2 1000mL 10g 5 2
18. 3 DNA RNA DEPC RNase Free 10mM Tris 1mM EDTA pH8 0 7 6 TE buffer DNA pH8 0 RNA pH7 6 S0 Advanced Microorganism Research 69505KEFO1I 8 1
19. 15 2 2mL 2mL 15000 rpm 3 1 3 1 150u L 70 10 RNA 70C DNA 70C 90 C 4 Scientific ndustries 2 Vortex2 DNA RNA
20. 5 400 uh L 2 1 Vortex phenol Tris buffer pipett 6 15000 rpm 3 DNA DNA RNA 1 DNA 200 u L 2mL 99 1000u L vortex 15000 rpm 3 2 70 1000u L 15000 rpm 3 DNA RNA 70 99 DEPC 3 200p L DEPC Option DNA RNA DNA 2u L NanoDrop 260nm 260nm
21. DNA RNA 70 99 DEPC 3 200u L DEPC Option DNA RNA DNA 2u L NanoDrop 260nm 260nm 280nm 260nm 230nm 1 2 MSDS 13 Advanced Microorganism Research 69503KFO1I C 2 DNA RNA 200L 200uL 200uL 2 2207N2 I TF SDS 70 90C 10 a 1 Vortex 1 1 15 000rpm 3 99 15 000rpm NSP 15 000rpm Vortex 3 Vor
22. RNA 707C DNA 70C 90C 3 Scientific Industries 2 Vortex2 DNA RNA 4 200u L SDS 70 10 5 400u L 2 1 Vortex 15000 rpm 3 DNA DNA RNA 1 DNA 200 u L 2mL 99 1000u L vortex 15000 rpm 3 2 70 1000u L 15000 rpm 3
23. DNA RNA 18 E 2 DNA RNA 20 F DNA RNA 21 F 1 DNA RNA 21 F 2 DNA RNA 21 F 3 DNA RNA 21 F 4 DNA RNA 22 G DNA RNA 23 H DNA RNA 24 HH 1 DNA RNA 24 H 2 DNA RNA 25 I DNA RNA 26 7 DNA PCR 30 8 31 9 32 10 32 Advanced Microorganism Research 695053KEFO1I 1 50 1 10mLX1 2 0 7296 2
24. RNA 70C DNA 70C 90C 3 Scientific Industries 2 vortex2 DNA RNA 4 200u L SDS 70 10 5 400u L 2 1 1 Vortex 6 15000 rpm 3 300u L 2mL 2ml 2 9 7 1mL 99 15000 rpm 2 8 70 1mL 15000 rpm 2 9
25. 99 DEPC 7 200u L DEPC Option DNA RNA DNA 2u L NanoDrop 260nm 260nm 280nm 260nm 230nm 1 2 MSDS 10 Advanced Microorganism Research 69503KFO1I B 3 DNA RNA 200guL 1 2mL 200u L 1 2 70 C 10
26. 10 DNA 55 5 11 200u L T10E1 buffer DNA Optional DNA RNA DNA 2u L NanoDrop 260nm 260nm 280nm 260nm 230nm DNA Humic acid DNA 260 280 gt 1 8 PCR DNA RNA 1 2 MSDS 26 Advanced Microorganism Research 69503KFO1I pmL
27. RNA DNA 2u L NanoDrop 260nm 260nm 280nm 260nm 230nm 1 2 MSDS 17 Advanced Microorganism Research 69503KFO1I E DNA RNA E 1 DNA RNA 200L 2mL 150 70 90 C 10 NM a EE N a Vortex 1 15 000rpm 3 2 999 709 lt 15 000rpm ra l5 000rpm Vortex 3 Vortex 3 9 RNase Free T10EB1
28. DNA RNA N 4 18 Advanced Microorganism 7osog7C 692502 7 1 200u L 2 1 200u L 3 70 90 10 RNA 70C DNA 70C 90C SDS 4 400u L 2 1 Vortex 15000 rpm 3 DNA DNA RNA 1 DNA 200 un L 2mL
29. 25ng DNA u g mL 260 nn A260 x x mm 10 DNA 50 DNA DNA 33 RNA 40 260 nm A260 280 nm A280 A280 1 8 PCR SDS
30. Option DNA RNA DNA 2u L NanoDrop 260nm 260nm 280nm 260nm 230nm 1 2 MSDS 12 Advanced Microorganism Research 69505KEFO1I C DNA RNA C 1 DNA RNA 1mL lmL hu 5000mm a 14mL Falcon A 1mnL 14mL Falcon tube 1mL 4500 xG 5 000rpm 15 1 150u L 2 70 90 C 10
31. Vortex 15000 rpm 3 DNA o Co oo DNA RNA 1 DNA 200 unL 2mL 99 1000u L vortex 15000 rpm 3 2 70 1000u L 15000 rpm 3 DNA RNA 70 99 DEPC 3 200u L DEPC Option DNA RNA DNA 2u L NanoDrop 260nm 260nm 280nm 260nm 230nm 1 2 MSDS
32. DNA A 1 DNA 200u L 5M NaCI 10u L 40u L 2 5 3 Washing Buffer 500u L 4 Washing Buffer 500u L 5 70 Washing 1000u L 6 100u L DEPC 7 5 28 Advanced Microorg
33. 99 DEPC 14 Advanced Microorganism Research 69505KEFO1I 3 200u L DEPC Option DNA RNA DNA 2u L NanoDrop 260nm 260nm 280nm 260nm 230nm 1 2 MSDS 15 Advanced Microorganism Research 69505KEFO1I D DNA RNA D 1 Culture bottle DNA RNA ee lmL lmL 15 000 rpm 15 3
34. DNA RNA 1 DNA 200u L 2mL 99 1000u L vortex 15000 rpm 3 2 70 1000u L 15000 rpm 3 DNA RNA 70 99 DEPC 3 200u L DEPC Option DNA RNA DNA 2u L NanoDrop 260nm 260nm 280nm 260nm 230nm 1 2 MSDS rg7
35. 1 70 C 10 Scientific Industries 2 Vortex2 DNA RNA 200u L SDS 70 C10 400u L 2 1 Vortex 15000 rpm 3 DNA o Co oo DNA RNA 1 DNA 200 unL 2mL 99 1000u L vortex 15000 rpm 3 2 70 1000u L 15000 rpm 3 3 200u L DEPC
36. A 30 1 1mL 2mL 1mL 30 4 C 90 2 12000g 15000 rpm 4 C 15 3 150 u L 1 70 90 10 RNA 70 DNA 70 90 4 Scientific Industries 2 Vortex2 DNA RNA 5 200u L SDS 70 10 6 400 un L 2 1 Vortex
37. phenol Tris buffer pipett 7 15000 rpm 3 DNA 200u L DNA RNA 1 DNA 200 L 2mL 99 1000u L vortex 15000 rpm 3 2 70 1000u L 15000 rpm 3 DNA RNA 70 99 DEPC 3 200p L DEPC Option DNA RNA DNA 2u L NanoDrop 260nm 260nm 280nm 260nm 230nm
38. 1000u L 15000 rpm 3 DNA RNA 70 99 DEPC 3 200u L DEPC Option DNA RNA DNA 2h L NanoDrop 260nm 260nm 280nm 260nm 230nm 1 2 MSDS 4 Advanced Microorganism Research 69505KEFO1I A 2 DNA RNA lg 2 2 3 9ml PBS 2mL 1g 9mLPBS 1 2 2mL
39. 15000 rpm 3 2 70 1000u L 15000 rpm 3 3 200u L DEPC Option DNA RNA DNA 2u L NanoDrop 260nm 260nm 280nm 260nm 230nm 1 2 MSDS 11 Advanced Microorganism Research 62502 7 B 4 DNA RNA a 2 HB 1mL 15 000 rpm 15 2mL A 30 kh 1mL 30 4 90 15000rpm 15 150u L
40. 50 3 SDS 10mLx1 4 20mLx 1 429 5 DEPC 10mLx 1 6 2mL 50 7 1 8 1 2 DNA RNA 3 gt 1 99 2 7096 999 DEPC 7 3 3 2nL 4 1000u L 200u L 5 RNase Free 10mM Tris 1mM EDTA pH8 0 pH7 6 TE buffer DNA RNA gt 1 70C 90C 90
41. 5 200u L SDS 70 10 6 400 L 2 1 Vortex 15000 rpm 3 DNA DNA RNA 1 DNA 200 uL 2mL 99 1000u L vortex 15000 rpm 3 2 70 1000u L 15000 rpm 3 DNA RNA 70 99 DEPC 3 200u L DEPC Option DNA RNA DNA 2u L NanoDrop 260nm 260nm 280nm 260nm 230nm
42. 1000u L 15000 rpm 3 DNA RNA 70 99 DEPC 3 200u L DEPC Option DNA RNA DNA 2u L NanoDrop 260nm 260nm 280nm 260nm 230nm 1 2 MSDS 7 Advanced Microorganism Research 69505KEFO1I B DNA RNA B 1 DNA RNA 6mL 2mL 2mL TC 15 Vortex 20 2mL 1 2mL 3
43. 1 2 MSDS 16 Advanced Microorganism Research 69505KEFO1I D 2 DNA RNA SW ml 9 SE 4 15 000 rpm 10 my 1mL 3 A 108 109 mL a 1 1mL 10 10 mL 1mL 12000g 15000 rpm 4 10 2 150 u L 1 3 Scientific Industries 2 Vortex2 DNA RNA 4 10000 r
44. 99 1000u L vortex 15000 rpm 3 2 70 1000u L 15000 rpm 3 DNA RNA 70 99 DEPC 3 200u L DEPC Option DNA RNA DNA 2u L NanoDrop 260nm 260nm 280nm 260nm 230nm 1 2 MSDS 19 rg7ceg Microorganism Research 62502 7 E 2 DNA RNA 1 2mL 2mL 15000 rpm 3
45. Millipore 2 100u L 150 3 4 150u L 1 5 70 90C 10 RNA 70C DNA 70C 90 Scientific Industries 2 vortex2 DNA RNA 200u L SDS 70 C10 400 u L 1 Vorte
46. 2 Scientific Industries 3 200 1000u L 20 200u L 2 20u L 4 Vortex 5 6 DNA RNA 7 1 Advanced Microorganism Research 695053KEFO1I 4 1 2mL 15 000 rpm 12000 xG 3 5 15 20 2 150u L
47. 4g 125mM NaCl 2mL 2mL 50mM EDTA pH8 0 lmL 20 5 10 15 000rpm A 150 uaL 15 000rp 5 70 90C 10 200L SDS 70 90C 60 10 aa a 23 ia Vortex 1 15 000rpm 3 3 99 709 i 15 000rpm REP 15 000rpm Vortex 3 Vortex 3 T10E1 DNA RNA 27 Advanced Microorganism Research 62502 7 DNA RNA DNA DNA Humic acid
48. TE buffer Advanced Microorganism Research 69503KFO1I 5 150u L B 150u L 2mL A 15 000 rpm 3 5 2mL 70 907C 70 90 C 10 2 10 15 000rpm lt Vortex 3 1 9996 1mL 709 1mL 15 000rpm 15 000rpm 3
49. 3 J DNA RNA my 3 Advanced Microorganism Research 69505KEFO1I 6 A DNA RNA A 1 DNA RNA 0 5 1g 2mL 4 5mL 9 PBS NR 0 5g 1g PBS 4 5 9mL 2mL 1 2mL 2mL 15000 rpm 3 150u L 1
50. 280nm 260nm 230nm 1 2 MSDS 9 Advanced Microorganism Research 69505KEFO1I H DNA RNA H 1 DNA RNA a Ss 2mL 8000rpm 20 my my my a 2 1 100mL 40mL tube 8000rpm 20 2 2mL 3 2mL 2mL 15000 rpm 3 DNA 4 150 up L 1
51. Advanced Microorganism Research 6 92602 7 7 2012 5 69503 DNA RNA MORA EXTRACT 1 1 2 1 3 1 4 2 5 3 6 4 A DNA RNA 4 A 1 DNA RNA 4 A 2 DNA RNA 5 A 3 RNA 6 B DNA RNA 8 B 1 DNA RNA 8 B 2 DNA RNA 9 B 3 DNA RNA 11 B 4 DNA RNA C DNA RNA 13 C 1 DNA RNA 13 C 2 DNA RNA 14 D DNA RNA 16 D 1 Culture Bottle DNA RNA 16 D 2 DNA RNA 17 Advanced Microorganism Research 69505KEFO1I E DNA RNA 18 E 1
52. anism Research 69505KEFO1I 2 5M NaCIl 40 5 uu PP DNA RNA 500uL Washing Buffer 500 L Washing Buffer 3 8 a Bp 2 a sep 1 1 1000L 70 a 1 NR 100uL DEPC my 29 DNA RNA Advanced Microorganism Research 69505KEFO1I 7 DNA PCR 1 DNA RNA DNA RNA 2 DNA DNA DNA PCR 1 DNA 100ng DNA
53. ceg Microorganism Research 69505KEFO1I A 3 RNA 0 5 a NINN lmL LmL 15 000 rpm my 2 a mm Pr 4 5mL PBS 2mL 30 200uL 200uL SDS 400 O 10 my my a a 1 15 000rpm Vortex 3 me 2mL 999 1mL 7096 1mL 15 000rpm 15 000rpm 3 3 J Zz DEPC TE buffer DNA RNA 1 0 5g 1g PBS 4 5 9mL 1mL RNA 2mL 2mL
54. mL 1 DNA RNA 1 DNA 200 u L 2mL 99 1000u L vortex 15000 rpm 3 2 70 1000u L 15000 rpm 3 DNA RNA 70 99 DEPC 3 200u L DEPC Option DNA RNA DNA 2u L NanoDrop 260nm 260nm 280nm 260nm 230nm 1 2 MSDS
55. pm 200u L SDS 70 C 10 5 400u L 2 1 Vortex phenol 6 15000 rpm 3 DNA DNA RNA 1 DNA RNA 200 u L 2mL 99 1000u L vortex 15000 rpm 3 2 70 1000u L 15000 rpm 3 DNA RNA 70 99 DEPC 3 200u L DEPC Option DNA
56. tex 3 gt gt DEPC TI10E1 DNA RNA 1 200u L 200u L 1 200u L SDS 2 70C 10 400u L 2 1 Vortex 3 15000 rpm 3 400u L DNA RNA DNA RNA 1 DNA RNA 400 u L 2mL 99 1000u L vortex 15000 rpm 3 2 70 1000u L 15000 rpm 3 DNA RNA 70
57. vanced Microorganism Research 69505KEFO1I 1 1mL 14mL tube 3 15 200u L 2 200u L 1 200u L SDS 200u L 70C 15 3 400u L 2 1 Vortex 4 15000 rpm 5 200u L DNA RNA DNA RNA 5 DNA 400 u L 2mL 99 1000u L vortex 15000 rpm 3 6 70 1000u L 15000 rpm 3 DNA RNA 70
58. x 15000 rpm 3 DNA gt CO DNA RNA 1 DNA 200 L 2mL 99 1000u L vortex 15000 rpm 3 2 70 1000u L 15000 rpm 3 DNA RNA 70 99 DEPC 3 200pn L DEPC Option DNA RNA DNA 2u L NanoDrop 260nm 260nm 280nm 260nm 230nm 1 2 MSDS
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